MD06G1004600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008559
Physical Location & Seq
Forward (+)
505500 .. 508832
3333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1004600.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGACTTAGAACCAAAAGACGATCCTCGTAAAGCTTCATTGAAACCATCATTTGCGGACCAGCATTTTCTAGTCAACTTCATAATGGGAAATTACTTGGGTCCTGATGTCAATATTGATAACCTGAAATACTCAGCATTTCAAAGGATAGTGGAAAGTTCGCCTCCATATATGTCAAGTGATTTGGGGCCATCATATGTCAGTGTGTCTCTTTTGGAGAGTTTATATTACTATCTCCTGAGAAATGCTCACCCTAGTCTCATTTTGAGACCAGATATGTTGCATAAGTACTTTAAGGGCAACCTAACCTTACCAAGTTCAGGGGTTACAAAAGAAAGTTGGCGCCAGTTCACAGATCTTTTTCCTTTGGCTCTTCATGAACAGATATGGTACCCAGAGAGCTTCAGAGTTGTTAAGGGGATTGTTTTGATTGATGATCCTGTTACATCATTTATGGAAGAAGATGTTCTTGAAAAGTTCAAATCTTTATCTGGTATAGATGGCCTGAAAATTGATATAGGTCAAACTATACGATATCAACATGAGTACCTGAATGGTGGAGAAAGTAAGTCTAATGGCTTGAATGATGATGGCAAGGAAACAAAGACAGAAAATATGTCTAATGGAAATGGTAATTCATCAGAAAGGTTTCTGAAAAAGTATAAGAGGAGGTGCGTCTCTCATTCTCCAGCAAAGCCAGCATCTCCCCAAGTTGTTTCTGAATCAAAGCATTATAACAAAGATGGAGCTTCATGGATGAGCAATCCAGATGGCCCTGCCCTGATGCCCCTTATCTCTCTTCCTAATATGGAAGAACCTACTTCTGATTCTTCTATTGTTTTGTCTGGGACTGCCAGGAAAGGGGTAGTTGGACCACCAGTTGGTTTAGTGGACATTGGTGTTAGCAAGGTTGCATATTACTTCCGGGTTGCTCTACCAGGGGTCAGGAAGGATTTTTGTCAGTTTAGTTGTGAGATTGAATCTAATGGGAAGGTTCATCTCCAAGGCATCACAAGTGGTGGAAATCCCATAAGGAAACGTTCGCGCGTATTTCAGATGAAGTTGCAGCAATTATGCCCAGCTGGACCATTCACTCTCTCTTTCAGCCTCCCTGGACCTGTTGATCCAAGGCTCTTCGCACCACACTTCGGATCTGATGGCATTTTTGAAGGAGTTATCGTAAAAGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009893 GO:0010604 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0060255 GO:0065007 GO:0080090 GO:1901535 GO:1901537
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

398

Amino Acids

44.11

Weight (kDa)

6.77

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 735
Acc65I GGTACC 1 cut(s) 390
AccB1I GGYRCC 2 cut(s) 342, 390
AccB7I CCANNNNNTGG 1 cut(s) 881
AccII CGCG 2 cut(s) 1045, 1047
AciI CCGC 1 cut(s) 56
AclI AACGTT 1 cut(s) 1039
AclWI GGATC 4 cut(s) 17, 431, 1118, 1159
AcuI CTGAAG 1 cut(s) 388
AcyI GRCGYC 1 cut(s) 343
AfaI GTAC 3 cut(s) 290, 392, 548
AfiI CCNNNNNNNGG 4 cut(s) 320, 861, 881, 1148
AgsI TTSAA 7 cut(s) 43, 143, 473, 481, 583, 980, 1169
AjnI CCWGG 3 cut(s) 854, 937, 1111
AluBI AGCT 4 cut(s) 35, 402, 749, 1082
AluI AGCT 4 cut(s) 35, 402, 749, 1082
Alw26I GTCTC 4 cut(s) 213, 262, 263, 682
AlwI GGATC 4 cut(s) 17, 431, 1118, 1159
AoxI GGCC 3 cut(s) 188, 502, 772
ApeKI GCWGC 1 cut(s) 1066
Asp700I GAANNNNTTC 2 cut(s) 465, 648
Asp718I GGTACC 1 cut(s) 390
AspLEI GCGC 2 cut(s) 345, 1047
AspS9I GGNCC 7 cut(s) 58, 101, 188, 773, 872, 1085, 1115
AsuC2I CCSGG 1 cut(s) 926
AsuHPI GGTGA 1 cut(s) 242
AvaII GGWCC 5 cut(s) 58, 101, 872, 1085, 1115
BaeI ACNNNNGTAYC 2 cut(s) 530, 563
BanI GGYRCC 2 cut(s) 342, 390
BbvI GCAGC 1 cut(s) 1078
BccI CCATC 7 cut(s) 55, 199, 494, 584, 737, 764, 1151
BciT130I CCWGG 3 cut(s) 856, 939, 1113
BcnI CCSGG 1 cut(s) 926
BcoDI GTCTC 4 cut(s) 213, 262, 263, 682
BfaI CTAG 2 cut(s) 71, 255
BfoI RGCGCY 1 cut(s) 346
BglII AGATCT 1 cut(s) 355
BisI GCNGC 1 cut(s) 1067
BlsI GCNGC 1 cut(s) 1068
BmcAI AGTACT 1 cut(s) 290
Bme1390I CCNGG 4 cut(s) 856, 926, 939, 1113
Bme18I GGWCC 5 cut(s) 58, 101, 872, 1085, 1115
BmgT120I GGNCC 7 cut(s) 58, 101, 188, 773, 872, 1085, 1115
BmiI GGNNCC 4 cut(s) 102, 189, 344, 392
BmrFI CCNGG 4 cut(s) 856, 926, 939, 1113
BmsI GCATC 3 cut(s) 710, 774, 1017
BplI GAGNNNNNCTC 2 cut(s) 232, 264
BpmI CTGGAG 1 cut(s) 672
BpuMI CCSGG 1 cut(s) 926
BsaHI GRCGYC 1 cut(s) 343
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 4 cut(s) 938, 1003, 1111, 1127
BsaXI ACNNNNNCTCC 2 cut(s) 1164, 1194
Bsc4I CCNNNNNNNGG 4 cut(s) 320, 861, 881, 1148
Bse1I ACTGG 2 cut(s) 346, 878
BseBI CCWGG 3 cut(s) 856, 939, 1113
BseDI CCNNGG 4 cut(s) 938, 1003, 1111, 1127
BseGI GGATG 1 cut(s) 762
BseLI CCNNNNNNNGG 4 cut(s) 320, 861, 881, 1148
BseMII CTCAG 2 cut(s) 147, 230
BseNI ACTGG 2 cut(s) 346, 878
BseRI GAGGAG 1 cut(s) 682
BseXI GCAGC 1 cut(s) 1078
BseYI CCCAGC 1 cut(s) 1078
Bsh1236I CGCG 2 cut(s) 1045, 1047
BshFI GGCC 3 cut(s) 190, 504, 774
BshNI GGYRCC 2 cut(s) 342, 390
BsiSI CCGG 1 cut(s) 925
BslFI GGGAC 1 cut(s) 862
BslI CCNNNNNNNGG 4 cut(s) 320, 861, 881, 1148
BsmAI GTCTC 4 cut(s) 213, 262, 263, 682
BsmBI CGTCTC 1 cut(s) 682
BsmFI GGGAC 1 cut(s) 862
BsnI GGCC 3 cut(s) 190, 504, 774
Bso31I GGTCTC 1 cut(s) 262
Bsp143I GATC 5 cut(s) 22, 355, 436, 1123, 1151
BspACI CCGC 1 cut(s) 56
BspANI GGCC 3 cut(s) 190, 504, 774
BspCNI CTCAG 2 cut(s) 146, 231
BspFNI CGCG 2 cut(s) 1045, 1047
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 4 cut(s) 102, 189, 344, 392
BspPI GGATC 4 cut(s) 17, 431, 1118, 1159
BspQI GCTCTTC 2 cut(s) 378, 1139
BspT107I GGYRCC 2 cut(s) 342, 390
BspTNI GGTCTC 1 cut(s) 262
BsrI ACTGG 2 cut(s) 346, 878
BssECI CCNNGG 4 cut(s) 938, 1003, 1111, 1127
BssMI GATC 5 cut(s) 22, 355, 436, 1123, 1151
BssNI GRCGYC 1 cut(s) 343
BssT1I CCWWGG 2 cut(s) 1003, 1127
Bst2UI CCWGG 3 cut(s) 856, 939, 1113
Bst6I CTCTTC 3 cut(s) 378, 804, 1139
BstACI GRCGYC 1 cut(s) 343
BstC8I GCNNGC 1 cut(s) 699
BstDEI CTNAG 3 cut(s) 7, 133, 239
BstF5I GGATG 1 cut(s) 762
BstFNI CGCG 2 cut(s) 1045, 1047
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 2 cut(s) 345, 1047
BstKTI GATC 5 cut(s) 25, 358, 439, 1126, 1154
BstMAI GTCTC 4 cut(s) 213, 262, 263, 682
BstMBI GATC 5 cut(s) 22, 355, 436, 1123, 1151
BstMWI GCNNNNNNNGC 1 cut(s) 698
BstNI CCWGG 3 cut(s) 856, 939, 1113
BstSCI CCNGG 4 cut(s) 854, 924, 937, 1111
BstUI CGCG 2 cut(s) 1045, 1047
BstV1I GCAGC 1 cut(s) 1078
BstX2I RGATCY 2 cut(s) 355, 1151
BstYI RGATCY 2 cut(s) 355, 1151
BsuRI GGCC 3 cut(s) 190, 504, 774
BtsCI GGATG 1 cut(s) 762
BtsIMutI CAGTG 1 cut(s) 208
Cac8I GCNNGC 1 cut(s) 699
CciI TCATGA 1 cut(s) 376
CfoI GCGC 2 cut(s) 345, 1047
Cfr13I GGNCC 7 cut(s) 58, 101, 188, 773, 872, 1085, 1115
CseI GACGC 1 cut(s) 664
Csp6I GTAC 3 cut(s) 289, 391, 547
CviAII CATG 3 cut(s) 377, 542, 753
CviQI GTAC 3 cut(s) 289, 391, 547
DdeI CTNAG 3 cut(s) 7, 133, 239
DinI GGCGCC 1 cut(s) 344
DpnI GATC 5 cut(s) 24, 357, 438, 1125, 1153
DpnII GATC 5 cut(s) 22, 355, 436, 1123, 1151
Eam1104I CTCTTC 3 cut(s) 378, 804, 1139
EarI CTCTTC 3 cut(s) 378, 804, 1139
Eco130I CCWWGG 2 cut(s) 1003, 1127
Eco31I GGTCTC 1 cut(s) 262
Eco32I GATATC 1 cut(s) 536
Eco47I GGWCC 5 cut(s) 58, 101, 872, 1085, 1115
Eco57I CTGAAG 1 cut(s) 388
EcoO109I RGGNCCY 1 cut(s) 101
EcoRII CCWGG 3 cut(s) 854, 937, 1111
EcoRV GATATC 1 cut(s) 536
EcoT14I CCWWGG 2 cut(s) 1003, 1127
EgeI GGCGCC 1 cut(s) 344
EheI GGCGCC 1 cut(s) 344
ErhI CCWWGG 2 cut(s) 1003, 1127
Esp3I CGTCTC 1 cut(s) 682
FaeI CATG 3 cut(s) 380, 545, 756
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FaqI GGGAC 1 cut(s) 862
FatI CATG 3 cut(s) 376, 541, 752
FauNDI CATATG 1 cut(s) 196
Fnu4HI GCNGC 1 cut(s) 1067
FokI GGATG 1 cut(s) 769
Fsp4HI GCNGC 1 cut(s) 1067
FspBI CTAG 2 cut(s) 71, 255
GlaI GCGC 2 cut(s) 344, 1046
GluI GCNGC 1 cut(s) 1067
GsaI CCCAGC 1 cut(s) 1082
GsuI CTGGAG 1 cut(s) 672
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 3 cut(s) 190, 504, 774
HapII CCGG 1 cut(s) 925
HgaI GACGC 1 cut(s) 664
HhaI GCGC 2 cut(s) 345, 1047
Hin1I GRCGYC 1 cut(s) 343
Hin1II CATG 3 cut(s) 380, 545, 756
Hin6I GCGC 2 cut(s) 343, 1045
HinP1I GCGC 2 cut(s) 343, 1045
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HindIII AAGCTT 1 cut(s) 33
HinfI GANTC 3 cut(s) 722, 827, 980
HpaII CCGG 1 cut(s) 925
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 3 cut(s) 76, 351, 892
Hpy188I TCNGA 8 cut(s) 407, 643, 654, 721, 826, 1056, 1151, 1156
Hpy188III TCNNGA 6 cut(s) 104, 238, 377, 470, 767, 946
Hpy8I GTNNAC 3 cut(s) 76, 351, 892
HpyAV CCTTC 3 cut(s) 943, 985, 1163
HpyCH4IV ACGT 1 cut(s) 1039
HpyCH4V TGCA 3 cut(s) 283, 914, 1066
HpyF10VI GCNNNNNNNGC 1 cut(s) 698
HpyF3I CTNAG 3 cut(s) 7, 133, 239
HpySE526I ACGT 1 cut(s) 1039
Hsp92I GRCGYC 1 cut(s) 343
Hsp92II CATG 3 cut(s) 380, 545, 756
HspAI GCGC 2 cut(s) 343, 1045
KasI GGCGCC 1 cut(s) 342
KpnI GGTACC 1 cut(s) 394
Kzo9I GATC 5 cut(s) 22, 355, 436, 1123, 1151
LguI GCTCTTC 2 cut(s) 378, 1139
LmnI GCTCC 1 cut(s) 746
Lsp1109I GCAGC 1 cut(s) 1078
LweI GCATC 3 cut(s) 710, 774, 1017
MaeI CTAG 2 cut(s) 71, 255
MaeII ACGT 1 cut(s) 1039
MaeIII GTNAC 2 cut(s) 325, 442
MalI GATC 5 cut(s) 24, 357, 438, 1125, 1153
MboI GATC 5 cut(s) 22, 355, 436, 1123, 1151
MboII GAAGA 7 cut(s) 365, 470, 473, 791, 822, 824, 1126
MflI RGATCY 2 cut(s) 355, 1151
MluCI AATT 4 cut(s) 91, 510, 634, 1070
Mly113I GGCGCC 1 cut(s) 343
MmeI TCCRAC 1 cut(s) 850
MnlI CCTC 5 cut(s) 36, 174, 660, 663, 1118
MroXI GAANNNNTTC 2 cut(s) 465, 648
MseI TTAA 2 cut(s) 294, 414
MspA1I CMGCKG 1 cut(s) 1082
MspI CCGG 1 cut(s) 925
MspR9I CCNGG 4 cut(s) 856, 926, 939, 1113
MvaI CCWGG 3 cut(s) 856, 939, 1113
MvnI CGCG 2 cut(s) 1045, 1047
MwoI GCNNNNNNNGC 1 cut(s) 698
NarI GGCGCC 1 cut(s) 343
NciI CCSGG 1 cut(s) 926
NdeI CATATG 1 cut(s) 196
NdeII GATC 5 cut(s) 22, 355, 436, 1123, 1151
NlaIII CATG 3 cut(s) 380, 545, 756
NlaIV GGNNCC 4 cut(s) 102, 189, 344, 392
PagI TCATGA 1 cut(s) 376
PciSI GCTCTTC 2 cut(s) 378, 1139
PcsI WCGNNNNNNNCGW 1 cut(s) 1185
PdmI GAANNNNTTC 2 cut(s) 465, 648
PfeI GAWTC 3 cut(s) 722, 827, 980
PflMI CCANNNNNTGG 1 cut(s) 881
PkrI GCNGC 1 cut(s) 1068
PluTI GGCGCC 1 cut(s) 346
PpuMI RGGWCCY 1 cut(s) 101
PsiI TTATAA 1 cut(s) 735
Psp1406I AACGTT 1 cut(s) 1039
Psp5II RGGWCCY 1 cut(s) 101
Psp6I CCWGG 3 cut(s) 854, 937, 1111
PspFI CCCAGC 1 cut(s) 1078
PspGI CCWGG 3 cut(s) 854, 937, 1111
PspN4I GGNNCC 4 cut(s) 102, 189, 344, 392
PspPI GGNCC 7 cut(s) 58, 101, 188, 773, 872, 1085, 1115
PspPPI RGGWCCY 1 cut(s) 101
PsuI RGATCY 2 cut(s) 355, 1151
PvuII CAGCTG 1 cut(s) 1082
RsaI GTAC 3 cut(s) 290, 392, 548
RsaNI GTAC 3 cut(s) 289, 391, 547
SapI GCTCTTC 2 cut(s) 378, 1139
SaqAI TTAA 2 cut(s) 294, 414
SatI GCNGC 1 cut(s) 1067
Sau3AI GATC 5 cut(s) 22, 355, 436, 1123, 1151
Sau96I GGNCC 7 cut(s) 58, 101, 188, 773, 872, 1085, 1115
ScaI AGTACT 1 cut(s) 290
ScrFI CCNGG 4 cut(s) 856, 926, 939, 1113
SfaNI GCATC 3 cut(s) 710, 774, 1017
SfoI GGCGCC 1 cut(s) 344
SinI GGWCC 5 cut(s) 58, 101, 872, 1085, 1115
Sse9I AATT 4 cut(s) 91, 510, 634, 1070
SsiI CCGC 1 cut(s) 56
SspDI GGCGCC 1 cut(s) 342
SspI AATATT 1 cut(s) 115
SspMI CTAG 2 cut(s) 71, 255
StyD4I CCNGG 4 cut(s) 854, 924, 937, 1111
StyI CCWWGG 2 cut(s) 1003, 1127
TaiI ACGT 1 cut(s) 1042
TasI AATT 4 cut(s) 91, 510, 634, 1070
TatI WGTACW 1 cut(s) 288
TfiI GAWTC 3 cut(s) 722, 827, 980
Tru1I TTAA 2 cut(s) 294, 414
Tru9I TTAA 2 cut(s) 294, 414
TscAI CASTG 1 cut(s) 208
TseI GCWGC 1 cut(s) 1066
TspDTI ATGAA 8 cut(s) 27, 70, 365, 393, 627, 741, 986, 1073
TspRI CASTG 1 cut(s) 208
Van91I CCANNNNNTGG 1 cut(s) 881
VpaK11BI GGWCC 5 cut(s) 58, 101, 872, 1085, 1115
XmnI GAANNNNTTC 2 cut(s) 465, 648
XspI CTAG 2 cut(s) 71, 255
ZrmI AGTACT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.