AT1G21640

NAD kinase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010084
Physical Location & Seq
Forward (+)
7588414 .. 7592890
4477 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G21640.1

Sequence Viewer

Length: 2958 bp
ATGTTCCTATGCTTTTGCCCTTGCCACGTACCTATCATGAGTCGCCTTTCTCCGGCCACCGGAATCTCTTCCCGCCTCCGCTTCTCCATTGGTTTGAGTTCCGATGGACGATTAATTCCCTTCGGATTCCGGTTTCGGAGAAACGATGTCCCGTTTAAACGCCGCTTGAGATTTGTGATCAGAGCGCAGCTCTCTGAAGCTTTTTCTCCCGATTTAGGTTTGGATTCTCAGGCTGTGAAATCCCGCGATACATCAAACTTGCCTTGGATTGGTCCAGTTCCAGGGGACATTGCTGAGGTTGAGGCGTATTGTAGAATTTTTAGATCAGCTGAGCGACTACATGGAGCGTTGATGGAGACACTATGCAACCCTGTGACTGGTGAATGTCGAGTACCGTATGATTTCTCACCGGAGGAAAAACCATTGTTGGAGGACAAAATAGTATCAGTGCTTGGTTGTATATTATCTCTTTTAAACAAAGGAAGGAAAGAAATTCTCTCTGGGAGGTCATCTTCTATGAATTCATTTAATTTGGATGATGTTGGGGTTGCAGAGGAGTCGCTTCCACCACTTGCTGTTTTCAGGGGTGAAATGAAACGGTGTTGTGAAAGCTTACACATTGCTCTTGAGAATTATCTGACGCCGGATGATGAGAGAAGTGGAATTGTTTGGAGGAAATTACAGAAGCTTAAAAATGTCTGCTACGACGCTGGTTTTCCACGCAGTGATAACTATCCTTGTCAAACACTTTTTGCGAATTGGGACCCTATTTACTCGTCAAATACGAAAGAGGATATTGATTCCTACGAGTCTGAGATTGCATTTTGGAGGGGAGGACAGGTAACCCAAGAAGGATTGAAGTGGTTGATAGAAAACGGATTTAAAACAATTGTTGACCTGAGAGCTGAAATCGTTAAGGATACATTCTACCAGACGGCACTTGATGATGCAATTTCCCTTGGGAAAATTACAGTGGTGCAAATTCCAATTGATGTCAGGATGGCTCCTAAAGCTGAGCAGGTCGAGCTGTTTGCTTCTATTGTATCAGATAGCAGCAAAAGACCGATATATGTTCACAGTAAAGAAGGTGTTTGGAGAACTTCTGCGATGGTTTCTAGGTGGAAGCAGTACATGACACGACCGATCACGAAAGAAATTCCAGTTTCAGAAGAGTCAAAGCGTCGGGAGGTTTCTGAAACTAAGCTTGGATCAAATGCTGTAGTATCTGGTAAGGGTGTACCTGATGAGCAGACTGATAAAGTCTCTGAAATCAATGAGGTTGATAGTAGATCTGCTTCAAGCCAGAGCAAGGAATCTGGAAGGTTTGAGGGAGATACATCTGCATCAGAATTTAATATGGTGAGCGATCCTCTTAAATCTCAAGTTCCACCAGGCAATATTTTTTCAAGAAAAGAAATGTCTAAATTCCTGAAGAGCAAAAGTATTGCTCCTGCTGGTTATCTTACTAATCCGTCCAAAATATTGGGAACAGTGCCAACTCCACAATTTTCATATACTGGTGTGACAAACGGAAATCAGATTGTTGATAAAGATTCGATAAGAAGACTTGCGGAGACAGGAAACTCCAATGGGACCCTTCTACCTACAAGTTCTCAAAGTTTAGATTTTGGCAACGGGAAGTTTTCAAATGGAAATGTGCATGCGTCTGATAACACCAACAAAAGTATATCGGACAACAGGGGAAATGGCTTCTCTGCAGCCCCTATTGCTGTGCCTCCTAGTGATAACTTAAGTCGCGCTGTAGGATCCCATTCGGTTCGAGAGTCTCAGACTCAGAGAAATAATAGTGGTTCCTCCTCGGATTCCAGTGATGATGAAGCTGGAGCTATTGAGGGAAATATGTGTGCTTCTGCCACTGGTGTAGTAAGGGTGCAGTCGAGAAAGAAAGCAGAGATGTTCTTAGTCCGAACTGATGGAGTGTCTTGTACAAGGGAAAAGGTGACAGAATCCTCTCTGGCCTTCACACATCCAAGTACTCAACAGCAGATGCTTCTTTGGAAAACTACCCCAAAAACTGTCTTACTGCTGAAGAAGCTCGGGCAAGAACTGATGGAGGAAGCTAAAGAGGCTGCCTCTTTCTTGTATCACCAAGAGAATATGAATGTTCTGGTTGAACCTGAGGTGCATGATGTATTTGCCAGGATTCCAGGGTTTGGCTTTGTCCAGACCTTCTACATTCAGGACACGAGCGATCTCCATGAAAGGGTTGATTTTGTGGCATGCTTAGGGGGGGATGGGGTGATATTACATGCATCAAACTTGTTCAAAGGAGCCGTCCCTCCCGTTGTTTCATTTAATCTGGGGTCTCTTGGATTTCTCACTTCACATCCATTTGAGGACTTCAGGCAAGACCTCAAACGAGTCATCCATGGGAATAACACGCTAGATGGGGTTTATATAACTCTTCGAATGCGTCTTCGTTGCGAAATCTATCGTAAAGGCAAAGCAATGCCTGGTAAAGTGTTTGATGTTCTGAACGAGATTGTTGTTGATCGAGGATCCAACCCATACCTTTCTAAGATCGAATGTTATGAGCACGACCGTCTTATCACGAAGGTACAAGGCGATGGAGTTATAGTAGCCACTCCTACAGGAAGTACTGCTTATTCTACAGCAGCAGGAGGTTCCATGGTGCATCCAAACGTTCCTTGCATGCTGTTCACTCCAATCTGCCCACATTCCCTGTCGTTCAGACCAGTTATACTTCCAGATTCTGCAAAACTCGAGTTAAAGATTCCAGATGATGCTCGAAGCAATGCATGGGTTTCGTTTGATGGAAAGAGAAGACAACAACTTTCAAGGGGAGATTCGGTGAGAATATACATGAGCCAACATCCACTCCCAACTGTCAACAAATCTGATCAAACCGGTGATTGGTTTAGAAGCTTAATCCGTTGCTTAAACTGGAACGAGCGTCTTGATCAAAAGGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

985

Amino Acids

109.19

Weight (kDa)

7.5

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-NADK PF22741 246 - 395 1.7e-62 DSP-PTPase phosphatase fused to NAD+ Kinase
Tc-R-P PF22785 280 - 374 1.4e-11 Polymorphic toxin system, DSP-PTPase phosphatase
NAD_kinase PF01513 686 - 798 3.8e-15 ATP-NAD kinase N-terminal domain
NAD_kinase_C PF20143 832 - 958 2.3e-35 ATP-NAD kinase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1009
AccB7I CCANNNNNTGG 1 cut(s) 2174
AccII CGCG 2 cut(s) 246, 1758
AciI CCGC 5 cut(s) 73, 79, 163, 244, 1571
AclI AACGTT 1 cut(s) 2664
AclWI GGATC 6 cut(s) 1216, 1361, 1761, 1774, 2514, 2527
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 7 cut(s) 315, 492, 520, 981, 1155, 1349, 1424
AcuI CTGAAG 4 cut(s) 216, 1451, 2069, 2347
AcyI GRCGYC 1 cut(s) 641
AdeI CACNNNGTG 1 cut(s) 725
AfaI GTAC 8 cut(s) 30, 393, 1130, 1239, 1948, 1996, 2580, 2620
AflII CTTAAG 1 cut(s) 1750
AgeI ACCGGT 1 cut(s) 2888
AgsI TTSAA 7 cut(s) 859, 1299, 1407, 1647, 2135, 2287, 2820
AjnI CCWGG 5 cut(s) 280, 1390, 2159, 2167, 2473
AjuI GAANNNNNNNTTGG 2 cut(s) 1188, 1220
Alw21I GWGCWC 1 cut(s) 2559
Alw26I GTCTC 5 cut(s) 350, 1267, 1568, 1791, 2331
AlwI GGATC 6 cut(s) 1216, 1361, 1761, 1774, 2514, 2527
AlwNI CAGNNNCTG 1 cut(s) 2735
Ama87I CYCGRG 2 cut(s) 2057, 2744
AoxI GGCC 2 cut(s) 54, 1977
ApeKI GCWGC 5 cut(s) 187, 1053, 1718, 2090, 2636
ApoI RAATTY 7 cut(s) 315, 492, 520, 981, 1155, 1349, 1424
ArsI GACNNNNNNTTYG 2 cut(s) 2280, 2312
AseI ATTAAT 1 cut(s) 113
AsiGI ACCGGT 1 cut(s) 2888
Asp700I GAANNNNTTC 1 cut(s) 67
AspLEI GCGC 2 cut(s) 187, 1760
AspS9I GGNCC 3 cut(s) 272, 763, 1593
AsuHPI GGTGA 9 cut(s) 392, 399, 599, 1372, 1972, 2099, 2272, 2845, 2903
AsuII TTCGAA 1 cut(s) 2428
AvaI CYCGRG 2 cut(s) 2057, 2744
AvaII GGWCC 3 cut(s) 272, 763, 1593
AxyI CCTNAGG 1 cut(s) 2139
BamHI GGATCC 2 cut(s) 1766, 2519
BarI GAAGNNNNNNTAC 2 cut(s) 1279, 1311
BauI CACGAG 1 cut(s) 2206
BbsI GAAGAC 3 cut(s) 1570, 2429, 2812
Bbv12I GWGCWC 1 cut(s) 2559
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 5 cut(s) 199, 1065, 1730, 2077, 2648
BceAI ACGGC 2 cut(s) 951, 2279
BcgI CGANNNNNNTGC 6 cut(s) 540, 574, 1013, 1047, 2769, 2803
BciT130I CCWGG 5 cut(s) 282, 1392, 2161, 2169, 2475
BciVI GTATCC 1 cut(s) 913
BclI TGATCA 3 cut(s) 177, 2881, 2941
BcoDI GTCTC 5 cut(s) 350, 1267, 1568, 1791, 2331
BfaI CTAG 3 cut(s) 1116, 1740, 2405
BfmI CTRYAG 5 cut(s) 1218, 1716, 1761, 2610, 2631
BfrI CTTAAG 1 cut(s) 1750
BfuAI ACCTGC 1 cut(s) 1009
BfuI GTATCC 1 cut(s) 913
BglII AGATCT 1 cut(s) 1289
BisI GCNGC 6 cut(s) 163, 188, 1054, 1719, 2091, 2637
BlpI GCTNAGC 2 cut(s) 330, 1014
BlsI GCNGC 6 cut(s) 164, 189, 1055, 1720, 2092, 2638
BmcAI AGTACT 2 cut(s) 1996, 2620
Bme1390I CCNGG 5 cut(s) 282, 1392, 2161, 2169, 2475
Bme18I GGWCC 3 cut(s) 272, 763, 1593
BmeT110I CYCGRG 2 cut(s) 2057, 2744
BmgT120I GGNCC 3 cut(s) 272, 763, 1593
BmrFI CCNGG 5 cut(s) 282, 1392, 2161, 2169, 2475
BmsI GCATC 6 cut(s) 937, 1352, 1998, 2282, 2665, 2755
BpiI GAAGAC 3 cut(s) 1570, 2429, 2812
BplI GAGNNNNNCTC 6 cut(s) 174, 206, 1354, 1386, 2078, 2110
BpmI CTGGAG 1 cut(s) 1863
Bpu10I CCTNAGC 2 cut(s) 294, 2245
Bpu1102I GCTNAGC 2 cut(s) 330, 1014
Bpu14I TTCGAA 1 cut(s) 2428
BpuEI CTTGAG 3 cut(s) 187, 647, 1365
BsaAI YACGTR 1 cut(s) 28
BsaBI GATNNNNATC 1 cut(s) 732
BsaHI GRCGYC 1 cut(s) 641
BsaI GGTCTC 1 cut(s) 2331
BsaJI CCNNGG 7 cut(s) 263, 281, 958, 1818, 2168, 2389, 2649
BsaWI WCCGGW 4 cut(s) 59, 129, 409, 2888
BsaXI ACNNNNNCTCC 8 cut(s) 825, 855, 2583, 2613, 2817, 2844, 2847, 2874
Bse118I RCCGGY 1 cut(s) 2888
Bse1I ACTGG 8 cut(s) 275, 382, 1160, 1522, 1827, 1882, 2717, 2930
Bse21I CCTNAGG 1 cut(s) 2139
Bse3DI GCAATG 4 cut(s) 288, 618, 2475, 2782
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 5 cut(s) 282, 1392, 2161, 2169, 2475
BseDI CCNNGG 7 cut(s) 263, 281, 958, 1818, 2168, 2389, 2649
BseGI GGATG 9 cut(s) 541, 652, 1005, 1987, 2260, 2347, 2385, 2656, 2854
BseJI GATNNNNATC 1 cut(s) 732
BseMI GCAATG 4 cut(s) 288, 618, 2475, 2782
BseMII CTCAG 9 cut(s) 242, 285, 321, 804, 890, 1005, 1802, 1808, 2130
BseNI ACTGG 8 cut(s) 275, 382, 1160, 1522, 1827, 1882, 2717, 2930
BseRI GAGGAG 2 cut(s) 569, 1807
BseXI GCAGC 5 cut(s) 199, 1065, 1730, 2077, 2648
BsgI GTGCAG 1 cut(s) 1913
Bsh1236I CGCG 2 cut(s) 246, 1758
Bsh1285I CGRYCG 2 cut(s) 1142, 2563
BshFI GGCC 2 cut(s) 56, 1979
BshTI ACCGGT 1 cut(s) 2888
BsiEI CGRYCG 2 cut(s) 1142, 2563
BsiHKAI GWGCWC 1 cut(s) 2559
BsiHKCI CYCGRG 2 cut(s) 2057, 2744
BsiSI CCGG 6 cut(s) 53, 60, 130, 410, 644, 2889
BslFI GGGAC 5 cut(s) 134, 299, 776, 1606, 2282
BsmAI GTCTC 5 cut(s) 350, 1267, 1568, 1791, 2331
BsmFI GGGAC 5 cut(s) 134, 299, 776, 1606, 2282
BsmI GAATGC 1 cut(s) 2436
BsnI GGCC 2 cut(s) 56, 1979
Bso31I GGTCTC 1 cut(s) 2331
BsoBI CYCGRG 2 cut(s) 2057, 2744
Bsp119I TTCGAA 1 cut(s) 2428
Bsp1286I GDGCHC 1 cut(s) 2559
Bsp1407I TGTACA 1 cut(s) 1946
Bsp1720I GCTNAGC 2 cut(s) 330, 1014
Bsp19I CCATGG 2 cut(s) 2389, 2649
BspACI CCGC 5 cut(s) 73, 79, 163, 244, 1571
BspANI GGCC 2 cut(s) 56, 1979
BspCNI CTCAG 9 cut(s) 241, 286, 322, 805, 891, 1006, 1801, 1807, 2131
BspFNI CGCG 2 cut(s) 246, 1758
BspHI TCATGA 1 cut(s) 36
BspMAI CTGCAG 1 cut(s) 1720
BspMI ACCTGC 1 cut(s) 1009
BspPI GGATC 6 cut(s) 1216, 1361, 1761, 1774, 2514, 2527
BspQI GCTCTTC 1 cut(s) 1427
BspT104I TTCGAA 1 cut(s) 2428
BspTI CTTAAG 1 cut(s) 1750
BspTNI GGTCTC 1 cut(s) 2331
BsrDI GCAATG 4 cut(s) 288, 618, 2475, 2782
BsrFI RCCGGY 1 cut(s) 2888
BsrGI TGTACA 1 cut(s) 1946
BsrI ACTGG 8 cut(s) 275, 382, 1160, 1522, 1827, 1882, 2717, 2930
BssAI RCCGGY 1 cut(s) 2888
BssECI CCNNGG 7 cut(s) 263, 281, 958, 1818, 2168, 2389, 2649
BssNI GRCGYC 1 cut(s) 641
BssSI CACGAG 1 cut(s) 2206
BssT1I CCWWGG 4 cut(s) 263, 958, 2389, 2649
Bst2BI CACGAG 1 cut(s) 2206
Bst2UI CCWGG 5 cut(s) 282, 1392, 2161, 2169, 2475
Bst4CI ACNGT 8 cut(s) 396, 600, 973, 1079, 1492, 2038, 2564, 2869
Bst6I CTCTTC 4 cut(s) 73, 1164, 1427, 2430
BstACI GRCGYC 1 cut(s) 641
BstAFI CTTAAG 1 cut(s) 1750
BstAUI TGTACA 1 cut(s) 1946
BstBAI YACGTR 1 cut(s) 28
BstBI TTCGAA 1 cut(s) 2428
BstC8I GCNNGC 3 cut(s) 1662, 2242, 2675
BstDSI CCRYGG 2 cut(s) 2389, 2649
BstEII GGTNACC 1 cut(s) 841
BstF5I GGATG 9 cut(s) 541, 652, 1005, 1987, 2260, 2347, 2385, 2656, 2854
BstFNI CGCG 2 cut(s) 246, 1758
BstHHI GCGC 2 cut(s) 187, 1760
BstMAI GTCTC 5 cut(s) 350, 1267, 1568, 1791, 2331
BstMCI CGRYCG 2 cut(s) 1142, 2563
BstMWI GCNNNNNNNGC 5 cut(s) 1010, 1024, 1727, 2053, 2087
BstNI CCWGG 5 cut(s) 282, 1392, 2161, 2169, 2475
BstNSI RCATGY 4 cut(s) 1664, 2244, 2273, 2677
BstPI GGTNACC 1 cut(s) 841
BstSCI CCNGG 5 cut(s) 280, 1390, 2159, 2167, 2473
BstSFI CTRYAG 5 cut(s) 1218, 1716, 1761, 2610, 2631
BstUI CGCG 2 cut(s) 246, 1758
BstV1I GCAGC 5 cut(s) 199, 1065, 1730, 2077, 2648
BstV2I GAAGAC 3 cut(s) 1570, 2429, 2812
BstX2I RGATCY 3 cut(s) 1289, 1766, 2519
BstXI CCANNNNNNTGG 1 cut(s) 1483
BstYI RGATCY 3 cut(s) 1289, 1766, 2519
Bsu36I CCTNAGG 1 cut(s) 2139
BsuI GTATCC 1 cut(s) 913
BsuRI GGCC 2 cut(s) 56, 1979
BtgI CCRYGG 2 cut(s) 2389, 2649
BtgZI GCGATG 2 cut(s) 1121, 2601
BtsCI GGATG 9 cut(s) 541, 652, 1005, 1987, 2260, 2347, 2385, 2656, 2854
BtsI GCAGTG 1 cut(s) 730
BtsIMutI CAGTG 6 cut(s) 453, 730, 978, 1497, 1834, 1875
BveI ACCTGC 1 cut(s) 1009
Cac8I GCNNGC 3 cut(s) 1662, 2242, 2675
CaiI CAGNNNCTG 1 cut(s) 2735
CciI TCATGA 1 cut(s) 36
CfoI GCGC 2 cut(s) 187, 1760
Cfr10I RCCGGY 1 cut(s) 2888
Cfr13I GGNCC 3 cut(s) 272, 763, 1593
CseI GACGC 6 cut(s) 649, 716, 1169, 1653, 2423, 2924
Csp6I GTAC 8 cut(s) 29, 392, 1129, 1238, 1947, 1995, 2579, 2619
CspAI ACCGGT 1 cut(s) 2888
CviQI GTAC 8 cut(s) 29, 392, 1129, 1238, 1947, 1995, 2579, 2619
DraI TTTAAA 3 cut(s) 157, 474, 883
DraIII CACNNNGTG 1 cut(s) 725
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 4 cut(s) 73, 1164, 1427, 2430
EarI CTCTTC 4 cut(s) 73, 1164, 1427, 2430
Eco130I CCWWGG 4 cut(s) 263, 958, 2389, 2649
Eco31I GGTCTC 1 cut(s) 2331
Eco47I GGWCC 3 cut(s) 272, 763, 1593
Eco57I CTGAAG 4 cut(s) 216, 1451, 2069, 2347
Eco81I CCTNAGG 1 cut(s) 2139
Eco88I CYCGRG 2 cut(s) 2057, 2744
Eco91I GGTNACC 1 cut(s) 841
EcoO109I RGGNCCY 2 cut(s) 763, 1593
EcoO65I GGTNACC 1 cut(s) 841
EcoRI GAATTC 1 cut(s) 520
EcoRII CCWGG 5 cut(s) 280, 1390, 2159, 2167, 2473
EcoT14I CCWWGG 4 cut(s) 263, 958, 2389, 2649
EcoT22I ATGCAT 2 cut(s) 2275, 2782
ErhI CCWWGG 4 cut(s) 263, 958, 2389, 2649
FalI AAGNNNNNCTT 2 cut(s) 2608, 2640
FaqI GGGAC 5 cut(s) 134, 299, 776, 1606, 2282
FauI CCCGC 2 cut(s) 80, 251
FbaI TGATCA 3 cut(s) 177, 2881, 2941
Fnu4HI GCNGC 6 cut(s) 163, 188, 1054, 1719, 2091, 2637
FokI GGATG 9 cut(s) 548, 659, 1012, 1974, 2267, 2334, 2372, 2643, 2841
Fsp4HI GCNGC 6 cut(s) 163, 188, 1054, 1719, 2091, 2637
FspBI CTAG 3 cut(s) 1116, 1740, 2405
GlaI GCGC 2 cut(s) 186, 1759
GluI GCNGC 6 cut(s) 163, 188, 1054, 1719, 2091, 2637
GsuI CTGGAG 1 cut(s) 1863
HaeIII GGCC 2 cut(s) 56, 1979
HapII CCGG 6 cut(s) 53, 60, 130, 410, 644, 2889
HgaI GACGC 6 cut(s) 649, 716, 1169, 1653, 2423, 2924
HhaI GCGC 2 cut(s) 187, 1760
Hin1I GRCGYC 1 cut(s) 641
Hin6I GCGC 2 cut(s) 185, 1758
HinP1I GCGC 2 cut(s) 185, 1758
HincII GTYRAC 2 cut(s) 895, 2872
HindII GTYRAC 2 cut(s) 895, 2872
HindIII AAGCTT 5 cut(s) 198, 610, 686, 1202, 2905
HpaII CCGG 6 cut(s) 53, 60, 130, 410, 644, 2889
HphI GGTGA 9 cut(s) 392, 399, 599, 1372, 1972, 2099, 2272, 2845, 2903
Hpy166II GTNNAC 5 cut(s) 895, 1075, 1238, 2682, 2872
Hpy8I GTNNAC 5 cut(s) 895, 1075, 1238, 2682, 2872
Hpy99I CGWCG 2 cut(s) 710, 1185
HpyAV CCTTC 9 cut(s) 130, 477, 845, 1079, 1314, 1607, 1990, 2200, 2569
HpyCH4III ACNGT 8 cut(s) 396, 600, 973, 1079, 1492, 2038, 2564, 2869
HpyCH4IV ACGT 2 cut(s) 27, 2664
HpyF10VI GCNNNNNNNGC 5 cut(s) 1010, 1024, 1727, 2053, 2087
HpySE526I ACGT 2 cut(s) 27, 2664
Hsp92I GRCGYC 1 cut(s) 641
HspAI GCGC 2 cut(s) 185, 1758
KflI GGGWCCC 2 cut(s) 763, 1593
Ksp22I TGATCA 3 cut(s) 177, 2881, 2941
LguI GCTCTTC 1 cut(s) 1427
LmnI GCTCC 5 cut(s) 344, 1009, 1453, 1844, 2291
Lsp1109I GCAGC 5 cut(s) 199, 1065, 1730, 2077, 2648
LweI GCATC 6 cut(s) 937, 1352, 1998, 2282, 2665, 2755
MaeI CTAG 3 cut(s) 1116, 1740, 2405
MaeII ACGT 2 cut(s) 27, 2664
MaeIII GTNAC 4 cut(s) 373, 841, 1522, 1960
MboII GAAGA 9 cut(s) 60, 504, 1181, 1444, 1575, 2062, 2417, 2429, 2817
MfeI CAATTG 2 cut(s) 888, 987
MflI RGATCY 3 cut(s) 1289, 1766, 2519
MhlI GDGCHC 1 cut(s) 2559
MlyI GAGTC 7 cut(s) 49, 566, 818, 1181, 1786, 1793, 2391
MmeI TCCRAC 2 cut(s) 408, 2547
Mph1103I ATGCAT 2 cut(s) 2275, 2782
MroXI GAANNNNTTC 1 cut(s) 67
MspA1I CMGCKG 1 cut(s) 329
MspCI CTTAAG 1 cut(s) 1750
MspI CCGG 6 cut(s) 53, 60, 130, 410, 644, 2889
MspR9I CCNGG 5 cut(s) 282, 1392, 2161, 2169, 2475
MssI GTTTAAAC 1 cut(s) 157
MunI CAATTG 2 cut(s) 888, 987
Mva1269I GAATGC 1 cut(s) 2436
MvaI CCWGG 5 cut(s) 282, 1392, 2161, 2169, 2475
MvnI CGCG 2 cut(s) 246, 1758
MwoI GCNNNNNNNGC 5 cut(s) 1010, 1024, 1727, 2053, 2087
NcoI CCATGG 2 cut(s) 2389, 2649
NmuCI GTSAC 3 cut(s) 373, 1522, 1960
NsiI ATGCAT 2 cut(s) 2275, 2782
NspI RCATGY 4 cut(s) 1664, 2244, 2273, 2677
NspV TTCGAA 1 cut(s) 2428
PaeI GCATGC 3 cut(s) 1664, 2244, 2677
PaeR7I CTCGAG 1 cut(s) 2744
PagI TCATGA 1 cut(s) 36
PciSI GCTCTTC 1 cut(s) 1427
PcsI WCGNNNNNNNCGW 1 cut(s) 782
PctI GAATGC 1 cut(s) 2436
PdmI GAANNNNTTC 1 cut(s) 67
PflMI CCANNNNNTGG 1 cut(s) 2174
PinAI ACCGGT 1 cut(s) 2888
PkrI GCNGC 6 cut(s) 164, 189, 1055, 1720, 2092, 2638
PleI GAGTC 7 cut(s) 48, 565, 817, 1180, 1786, 1792, 2390
PmeI GTTTAAAC 1 cut(s) 157
PpsI GAGTC 7 cut(s) 48, 565, 817, 1180, 1786, 1792, 2390
Ppu21I YACGTR 1 cut(s) 28
PpuMI RGGWCCY 2 cut(s) 763, 1593
PshBI ATTAAT 1 cut(s) 113
Psp1406I AACGTT 1 cut(s) 2664
Psp5II RGGWCCY 2 cut(s) 763, 1593
Psp6I CCWGG 5 cut(s) 280, 1390, 2159, 2167, 2473
PspEI GGTNACC 1 cut(s) 841
PspGI CCWGG 5 cut(s) 280, 1390, 2159, 2167, 2473
PspPI GGNCC 3 cut(s) 272, 763, 1593
PspPPI RGGWCCY 2 cut(s) 763, 1593
PspXI VCTCGAGB 1 cut(s) 2744
PstI CTGCAG 1 cut(s) 1720
PstNI CAGNNNCTG 1 cut(s) 2735
PsuI RGATCY 3 cut(s) 1289, 1766, 2519
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 8 cut(s) 30, 393, 1130, 1239, 1948, 1996, 2580, 2620
RsaNI GTAC 8 cut(s) 29, 392, 1129, 1238, 1947, 1995, 2579, 2619
SapI GCTCTTC 1 cut(s) 1427
SatI GCNGC 6 cut(s) 163, 188, 1054, 1719, 2091, 2637
Sau96I GGNCC 3 cut(s) 272, 763, 1593
ScaI AGTACT 2 cut(s) 1996, 2620
SchI GAGTC 7 cut(s) 49, 566, 818, 1181, 1786, 1793, 2391
ScrFI CCNGG 5 cut(s) 282, 1392, 2161, 2169, 2475
SduI GDGCHC 1 cut(s) 2559
SfaNI GCATC 6 cut(s) 937, 1352, 1998, 2282, 2665, 2755
SfcI CTRYAG 5 cut(s) 1218, 1716, 1761, 2610, 2631
Sfr274I CTCGAG 1 cut(s) 2744
SfuI TTCGAA 1 cut(s) 2428
SinI GGWCC 3 cut(s) 272, 763, 1593
SlaI CTCGAG 1 cut(s) 2744
SmlI CTYRAG 5 cut(s) 166, 626, 1380, 1750, 2744
SmoI CTYRAG 5 cut(s) 166, 626, 1380, 1750, 2744
SphI GCATGC 3 cut(s) 1664, 2244, 2677
SsiI CCGC 5 cut(s) 73, 79, 163, 244, 1571
SspI AATATT 2 cut(s) 1399, 1482
SspMI CTAG 3 cut(s) 1116, 1740, 2405
StyD4I CCNGG 5 cut(s) 280, 1390, 2159, 2167, 2473
StyI CCWWGG 4 cut(s) 263, 958, 2389, 2649
TaaI ACNGT 8 cut(s) 396, 600, 973, 1079, 1492, 2038, 2564, 2869
TaiI ACGT 2 cut(s) 30, 2667
TaqII GACCGA 2 cut(s) 1078, 1156
TatI WGTACW 4 cut(s) 1128, 1946, 1994, 2618
TauI GCSGC 1 cut(s) 165
TscAI CASTG 6 cut(s) 453, 730, 978, 1497, 1834, 1882
TseFI GTSAC 3 cut(s) 373, 1522, 1960
TseI GCWGC 5 cut(s) 187, 1053, 1718, 2090, 2636
Tsp45I GTSAC 3 cut(s) 373, 1522, 1960
TspDTI ATGAA 8 cut(s) 513, 533, 608, 1500, 1851, 2135, 2235, 2301
TspGWI ACGGA 4 cut(s) 891, 1461, 1545, 2903
TspRI CASTG 6 cut(s) 453, 730, 978, 1497, 1834, 1882
Van91I CCANNNNNTGG 1 cut(s) 2174
Vha464I CTTAAG 1 cut(s) 1750
VpaK11BI GGWCC 3 cut(s) 272, 763, 1593
VspI ATTAAT 1 cut(s) 113
XapI RAATTY 7 cut(s) 315, 492, 520, 981, 1155, 1349, 1424
XceI RCATGY 4 cut(s) 1664, 2244, 2273, 2677
XhoI CTCGAG 1 cut(s) 2744
XmnI GAANNNNTTC 1 cut(s) 67
XspI CTAG 3 cut(s) 1116, 1740, 2405
ZrmI AGTACT 2 cut(s) 1996, 2620
Zsp2I ATGCAT 2 cut(s) 2275, 2782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.