Rh6BG384200

NAD kinase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010084
Physical Location & Seq
Reverse (-)
61287817 .. 61292312
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG384200.1

Sequence Viewer

Length: 3003 bp
ATGGTGGTATGTTGCCTCATGTGCCAGGTGGCCATCGCCGTCGATATGAGCCGCTGTGCCGCTTCACCTCCCAATCTGTGCCCCCTCCAGTTCTCAGGTAGCCCCGCCAAGCTATTCGGATTCGGGTTCGACATTCAGAAGAAGAGGCGGCTCAAGTTCGTCGTCAGTGCGGAGCTGTCCAAGCCATTCTCTCTCAGTTTCGGCTTGGACTCTCAGGGCTATCAGCTTCATGATCCGTCGCAGTCGCGGAGGATTGGCCCTGTTCCTGGGGATATTGCAGAGATTGAAGCCTATTGTAGAATTTTTCGAAGTGCTGAACGACTGCATACTGTATTGATGGAGACATTGTGCAATCCGGTGACCGGGGTGTGCAGTGTTTATTATGACTTCCCATCGGAGGAAAAGCCGCTCTTGGAGGATAAGATTGTGTCTGTGATTGGTTGCATGATGTCACTGTTGAACAAAGCAAGGGAGGATGTGCTTTCGGGAAGATCAGCCATTATGAGCTCCTTTCGAGGAGTTGTGGATGTCGGTGTGGTGGAGGAAGACATGCTTCCTCCGCTTGCTGTTTTCAGGAGTGAGCTGAAGAGGTGCTGCGAGAGCTTGCACGTTGCGCTTGAGGACTGGTTGATGCCTGGGGATGAGCAGAGCTTGGATGTGTGGAGGAAACTTCAGAGGCTGAAGAATGTGTGTTATGATTCCGGTTTTCCACGCGAGGAGGATTATCCGTGCAATACGCTTTTTGCTAATTGGACTCCTGTTTACTTATCCTCTTCTAAAGAGGATGTTAGGTCCGAAGATTCGGAGGTAGCGTTTTGGAGGGGTGGCCAGGTAACAGAAGAAGGTCTGGAGTGGTTGCTGGAGAAAGGATTTAAAACTATTGTTGATCTTAGGGCAGAAATTGTAAAAGACAATATATATCAGGCAGCCGTGGATGATGCTATTGCATCAGGGAAGGTGGAATTGATCAGAATTCCAGTTGAAGTTGGGACTGCACCTTCAATGGAGCAGGTTGAGAAATTAGCAAGTTTGGTTTCAGATTGCAGCAAAAAGCCAATCTATCTTCATAGCAAGGAAGGTCTCTGGAGAACATCTGCCATGGTTTCCAGATGGAGGCAGCACTTGACTCGCCGTGCTGTTCAGTTTGTCTCCAAGCAGTCGGTTTCTCTTAATGGAGAAGTTGAAAAACCCTCAACTATGCAGAAAAGTTCCCTCCTTGAAAAGAAGAATGAATCACTGGACACAATTCATGGTTCAAATGGGGTGCTACAAAAAAATGATTCTGTAGACAGGGATGAAGCAAATCTAAATGGGACCTGCAATAGCCTTATATCTATCCAAGGTTTGAGATCAGTTGAATCAGATGAAAGTGAACCTTTGGTGAACTTCTGCAGGGAAGTTGACCCCCTAAATGCTCAGGTTCCTCCTTGCAATATTTTCTCAAGAAAAGAAATGTCCAGGTTTCTTGAGAGAAAAAATATTGCACCACTCACTTATTTTAATTATCAGTTAAATAGGTTGAAGGCATTACCAATTTCCAGGTATATGAATACTAAGATAATGTGGAGAGGTGAGATTGTTGATACTGATCCAGTGCGGGAGGTTGTGGAGGCAGAAAATTCCAATGAGATACCTGATGCAAAACATCTGCTTCCAGAGTTCCAGATCTCTGCTTCTGGCAATGGGAAGTATTTGACCGGTGTTGGTAATGGTTCTGTAACTGCAGTTGTAAATGGATTTGGTGAAGGGGAGAAAGGTTCCTTGTTAGCCGCTAATTTGTCTACTAACGCGAGCAACAATCATTCTGAGAGTGTCTTATCTAAAGCAGTGAAGGAGGAAAGGAAGAGTAATGGTAGAGCCCCTTTAGTTTCAAGTGATGATGAATTGGGATCAATTGAAGGAGATATGTGTGCTTCTGCAACTGGGGTTGTAAGGGTGCAGTCAAGAAAGAAAGCAGAGATGTTTTTAGTACGAACAGATGGATTCTCTTGTACCCGAGAAAAAGTGACAGAATCCTCCCTGGCCTTCACTCATCCAAGTACTCAGCAACAAATGCTTATGTGGAAATCTACACCAAAGACAGTATTAGTGTTAAAGAAGCTTGGGCATGAGCTTATGGAACAAGCTAAAGAGGTTGTCTCCTTCTTGTATTACCAAGAGAAAATGAATGTTCTGGTGGAACCTGACTTGCATGATGTATTTGCTAGAATCCCAGGTTTTGGATTTGTTCAGACCTTCTACACCCAAGATACCGGTGATCTGCATGAGAGGGTTGATTTTGTGGCATGTCTGGGAGGGGATGGGGTAATACTCCATGCTTCAAATCTATTCAAAGGTGCTGTTCCCCCCATCGTATCATTTAATCTTGGATCTCTTGGATTTTTGACTTCCCATACTTTTGATGACTACAGGCAGGACTTAAGACAGGTTATCCATGGAAATAACACAAGTGATGGTGTTTATATAACTCTCAGAATGCGTCTCAGGTGTGAAATTTTTCGAAATGGTAAAGCGATGCCTGGAAAGGTATTTGATGTTTTGAATGAAATAGTTGTTGATCGAGGTTCGAATCCGTACCTTTCTAAGATTGAATGTTATGAACGTGACCAGCTTATAACCAAGGTCCAAGGTGATGGTGTCATTGTAGCCACACCTACAGGAAGTACTGCTTATTCTACAGCTGCAGGAGGTTCCATGGTGCATCCAAATGTTCCTTGCATGTTATTTACACCAATCTGCCCACACTCCCTCTCATTTAGACCAGTTATACTTCCAGATTCTGCAAGCCTTGAATTAAAGATTCCAGAGGATGCTCGAAGCAATGCCTGGGTTTCTTTTGATGGGAAGAGAAGGCAGCAGCTCTCAAGGGGAGACTCTGTTCGAATAGCTATGAGCCAACACCCACTTCCAACAGTCAACAAGCGTGACCAAACAGGGGACTGGTTTCGAAGCTTGATTCGTTGCCTAAATTGGAATGAGAGGCTAGATCAAAAGGCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

1000

Amino Acids

111.4

Weight (kDa)

6.01

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-NADK PF22741 243 - 389 2.4e-64 DSP-PTPase phosphatase fused to NAD+ Kinase
Tc-R-P PF22785 277 - 371 1.1e-10 Polymorphic toxin system, DSP-PTPase phosphatase
NAD_kinase PF01513 703 - 813 5.4e-15 ATP-NAD kinase N-terminal domain
NAD_kinase_C PF20143 847 - 973 6.5e-35 ATP-NAD kinase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2615
Acc36I ACCTGC 2 cut(s) 1000, 1325
AccB7I CCANNNNNTGG 1 cut(s) 2219
AccBSI CCGCTC 1 cut(s) 409
AccI GTMKAC 2 cut(s) 1287, 1781
AccII CGCG 3 cut(s) 247, 714, 1790
AclWI GGATC 4 cut(s) 227, 1583, 1897, 2377
AcoI YGGCCR 2 cut(s) 30, 826
AcsI RAATTY 4 cut(s) 300, 972, 1618, 2493
AcuI CTGAAG 3 cut(s) 605, 656, 701
AfaI GTAC 5 cut(s) 1971, 1993, 2041, 2576, 2665
AfiI CCNNNNNNNGG 6 cut(s) 266, 362, 397, 1113, 2219, 2935
AflII CTTAAG 1 cut(s) 2419
AgeI ACCGGT 2 cut(s) 1697, 2252
AjuI GAANNNNNNNTTGG 2 cut(s) 2889, 2921
AloI GAACNNNNNNTCC 2 cut(s) 2862, 2894
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 6 cut(s) 335, 1085, 1153, 2143, 2486, 2865
AlwI GGATC 4 cut(s) 227, 1583, 1897, 2377
AlwNI CAGNNNCTG 2 cut(s) 679, 2780
Ama87I CYCGRG 1 cut(s) 1995
AoxI GGCC 5 cut(s) 30, 256, 826, 2022, 2994
ApeKI GCWGC 7 cut(s) 594, 926, 1044, 1117, 2681, 2854, 2857
ApoI RAATTY 4 cut(s) 300, 972, 1618, 2493
AsiGI ACCGGT 2 cut(s) 1697, 2252
Asp700I GAANNNNTTC 1 cut(s) 2496
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 5 cut(s) 257, 792, 1314, 2623, 2995
AsuC2I CCSGG 1 cut(s) 364
AsuHPI GGTGA 7 cut(s) 57, 370, 1393, 1583, 1754, 2267, 2642
AsuII TTCGAA 5 cut(s) 307, 2500, 2567, 2881, 2947
AvaI CYCGRG 1 cut(s) 1995
AvaII GGWCC 3 cut(s) 792, 1314, 2623
BaeGI GKGCMC 1 cut(s) 83
BalI TGGCCA 2 cut(s) 32, 828
BanII GRGCYC 2 cut(s) 509, 1861
BbsI GAAGAC 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 509
BbvI GCAGC 7 cut(s) 581, 938, 1056, 1129, 2668, 2866, 2869
BceAI ACGGC 3 cut(s) 23, 914, 1116
BcgI CGANNNNNNTGC 2 cut(s) 1109, 1143
BclI TGATCA 1 cut(s) 966
BcnI CCSGG 1 cut(s) 364
BcoDI GTCTC 6 cut(s) 335, 1085, 1153, 2143, 2486, 2865
BfaI CTAG 2 cut(s) 2205, 2984
BfmI CTRYAG 7 cut(s) 1284, 1390, 1722, 2407, 2655, 2676, 2682
BfrI CTTAAG 1 cut(s) 2419
BfuAI ACCTGC 2 cut(s) 1000, 1325
BglII AGATCT 1 cut(s) 1665
BmcAI AGTACT 2 cut(s) 2041, 2665
Bme18I GGWCC 3 cut(s) 792, 1314, 2623
BmeT110I CYCGRG 1 cut(s) 1995
BmgT120I GGNCC 5 cut(s) 257, 792, 1314, 2623, 2995
BmiI GGNNCC 5 cut(s) 1315, 1422, 1759, 2181, 2692
BmrI ACTGGG 1 cut(s) 1932
BmsI GCATC 7 cut(s) 621, 928, 956, 1627, 2505, 2710, 2800
BmuI ACTGGG 1 cut(s) 1932
BpiI GAAGAC 1 cut(s) 552
BplI GAGNNNNNCTC 2 cut(s) 2123, 2155
BpmI CTGGAG 4 cut(s) 71, 869, 881, 1105
Bpu10I CCTNAGC 1 cut(s) 1416
Bpu14I TTCGAA 5 cut(s) 307, 2500, 2567, 2881, 2947
BpuEI CTTGAG 5 cut(s) 137, 638, 1426, 1487, 2848
BpuMI CCSGG 1 cut(s) 364
BsaBI GATNNNNATC 1 cut(s) 1587
BsaI GGTCTC 1 cut(s) 1085
BsaWI WCCGGW 4 cut(s) 355, 701, 1697, 2252
BsaXI ACNNNNNCTCC 6 cut(s) 332, 362, 797, 827, 2862, 2892
Bsc4I CCNNNNNNNGG 6 cut(s) 266, 362, 397, 1113, 2219, 2935
Bse118I RCCGGY 2 cut(s) 1697, 2252
Bse1I ACTGG 8 cut(s) 88, 629, 977, 1242, 1592, 1927, 2762, 2945
Bse3DI GCAATG 2 cut(s) 1687, 2827
Bse8I GATNNNNATC 1 cut(s) 1587
BseJI GATNNNNATC 1 cut(s) 1587
BseLI CCNNNNNNNGG 6 cut(s) 266, 362, 397, 1113, 2219, 2935
BseMI GCAATG 2 cut(s) 1687, 2827
BseMII CTCAG 8 cut(s) 108, 208, 227, 1430, 1797, 2057, 2485, 2497
BseNI ACTGG 8 cut(s) 88, 629, 977, 1242, 1592, 1927, 2762, 2945
BseRI GAGGAG 2 cut(s) 531, 731
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 7 cut(s) 581, 938, 1056, 1129, 2668, 2866, 2869
BsgI GTGCAG 3 cut(s) 391, 978, 1958
Bsh1236I CGCG 3 cut(s) 247, 714, 1790
BshFI GGCC 5 cut(s) 32, 258, 828, 2024, 2996
BshTI ACCGGT 2 cut(s) 1697, 2252
BsiHKAI GWGCWC 1 cut(s) 509
BsiHKCI CYCGRG 1 cut(s) 1995
BsiSI CCGG 5 cut(s) 356, 363, 702, 1698, 2253
BslFI GGGAC 3 cut(s) 1003, 1327, 2951
BslI CCNNNNNNNGG 6 cut(s) 266, 362, 397, 1113, 2219, 2935
BsmAI GTCTC 6 cut(s) 335, 1085, 1153, 2143, 2486, 2865
BsmBI CGTCTC 1 cut(s) 2486
BsmFI GGGAC 3 cut(s) 1003, 1327, 2951
BsmI GAATGC 1 cut(s) 2481
BsnI GGCC 5 cut(s) 32, 258, 828, 2024, 2996
Bso31I GGTCTC 1 cut(s) 1085
BsoBI CYCGRG 1 cut(s) 1995
Bsp119I TTCGAA 5 cut(s) 307, 2500, 2567, 2881, 2947
Bsp1286I GDGCHC 3 cut(s) 83, 509, 1861
Bsp19I CCATGG 3 cut(s) 1098, 2434, 2694
BspANI GGCC 5 cut(s) 32, 258, 828, 2024, 2996
BspCNI CTCAG 8 cut(s) 107, 207, 226, 1429, 1798, 2056, 2484, 2496
BspFNI CGCG 3 cut(s) 247, 714, 1790
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 5 cut(s) 1315, 1422, 1759, 2181, 2692
BspMAI CTGCAG 3 cut(s) 1394, 1726, 2686
BspMI ACCTGC 2 cut(s) 1000, 1325
BspPI GGATC 4 cut(s) 227, 1583, 1897, 2377
BspT104I TTCGAA 5 cut(s) 307, 2500, 2567, 2881, 2947
BspTI CTTAAG 1 cut(s) 2419
BspTNI GGTCTC 1 cut(s) 1085
BsrBI CCGCTC 1 cut(s) 409
BsrDI GCAATG 2 cut(s) 1687, 2827
BsrFI RCCGGY 2 cut(s) 1697, 2252
BsrI ACTGG 8 cut(s) 88, 629, 977, 1242, 1592, 1927, 2762, 2945
BssAI RCCGGY 2 cut(s) 1697, 2252
BssT1I CCWWGG 6 cut(s) 1098, 1339, 2434, 2619, 2626, 2694
Bst4CI ACNGT 4 cut(s) 331, 456, 2083, 2914
Bst6I CTCTTC 5 cut(s) 137, 581, 778, 1838, 2840
BstAFI CTTAAG 1 cut(s) 2419
BstAPI GCANNNNNTGC 1 cut(s) 2053
BstBI TTCGAA 5 cut(s) 307, 2500, 2567, 2881, 2947
BstC8I GCNNGC 4 cut(s) 564, 605, 1792, 2785
BstDSI CCRYGG 4 cut(s) 930, 1098, 2434, 2694
BstEII GGTNACC 1 cut(s) 358
BstFNI CGCG 3 cut(s) 247, 714, 1790
BstHHI GCGC 1 cut(s) 616
BstMAI GTCTC 6 cut(s) 335, 1085, 1153, 2143, 2486, 2865
BstMWI GCNNNNNNNGC 6 cut(s) 21, 181, 559, 600, 613, 2053
BstNSI RCATGY 3 cut(s) 553, 2289, 2722
BstPI GGTNACC 1 cut(s) 358
BstSFI CTRYAG 7 cut(s) 1284, 1390, 1722, 2407, 2655, 2676, 2682
BstSLI GKGCMC 1 cut(s) 83
BstUI CGCG 3 cut(s) 247, 714, 1790
BstV1I GCAGC 7 cut(s) 581, 938, 1056, 1129, 2668, 2866, 2869
BstV2I GAAGAC 1 cut(s) 552
BstX2I RGATCY 2 cut(s) 1665, 2369
BstXI CCANNNNNNTGG 1 cut(s) 2633
BstYI RGATCY 2 cut(s) 1665, 2369
BsuRI GGCC 5 cut(s) 32, 258, 828, 2024, 2996
BtgI CCRYGG 4 cut(s) 930, 1098, 2434, 2694
BtgZI GCGATG 2 cut(s) 19, 2528
BtsI GCAGTG 2 cut(s) 379, 1833
BtsIMutI CAGTG 6 cut(s) 172, 379, 452, 1235, 1599, 1833
BveI ACCTGC 2 cut(s) 1000, 1325
Cac8I GCNNGC 4 cut(s) 564, 605, 1792, 2785
CaiI CAGNNNCTG 2 cut(s) 679, 2780
CciI TCATGA 1 cut(s) 229
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 2 cut(s) 1697, 2252
Cfr13I GGNCC 5 cut(s) 257, 792, 1314, 2623, 2995
CseI GACGC 1 cut(s) 2468
Csp6I GTAC 5 cut(s) 1970, 1992, 2040, 2575, 2664
CspAI ACCGGT 2 cut(s) 1697, 2252
CviQI GTAC 5 cut(s) 1970, 1992, 2040, 2575, 2664
DraI TTTAAA 1 cut(s) 874
EaeI YGGCCR 2 cut(s) 30, 826
Eam1104I CTCTTC 5 cut(s) 137, 581, 778, 1838, 2840
EarI CTCTTC 5 cut(s) 137, 581, 778, 1838, 2840
Ecl136II GAGCTC 1 cut(s) 507
Eco130I CCWWGG 6 cut(s) 1098, 1339, 2434, 2619, 2626, 2694
Eco24I GRGCYC 2 cut(s) 509, 1861
Eco31I GGTCTC 1 cut(s) 1085
Eco47I GGWCC 3 cut(s) 792, 1314, 2623
Eco53kI GAGCTC 1 cut(s) 507
Eco57I CTGAAG 3 cut(s) 605, 656, 701
Eco88I CYCGRG 1 cut(s) 1995
Eco91I GGTNACC 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 507
EcoO109I RGGNCCY 2 cut(s) 1314, 2995
EcoO65I GGTNACC 1 cut(s) 358
EcoRI GAATTC 1 cut(s) 972
EcoT14I CCWWGG 6 cut(s) 1098, 1339, 2434, 2619, 2626, 2694
EcoT38I GRGCYC 2 cut(s) 509, 1861
ErhI CCWWGG 6 cut(s) 1098, 1339, 2434, 2619, 2626, 2694
Esp3I CGTCTC 1 cut(s) 2486
FalI AAGNNNNNCTT 8 cut(s) 395, 427, 537, 569, 1360, 1392, 2653, 2685
FaqI GGGAC 3 cut(s) 1003, 1327, 2951
FauI CCCGC 2 cut(s) 112, 1590
FbaI TGATCA 1 cut(s) 966
FblI GTMKAC 2 cut(s) 1287, 1781
FriOI GRGCYC 2 cut(s) 509, 1861
FspBI CTAG 2 cut(s) 2205, 2984
GlaI GCGC 1 cut(s) 615
GsuI CTGGAG 4 cut(s) 71, 869, 881, 1105
HaeIII GGCC 5 cut(s) 32, 258, 828, 2024, 2996
HapII CCGG 5 cut(s) 356, 363, 702, 1698, 2253
HgaI GACGC 1 cut(s) 2468
HhaI GCGC 1 cut(s) 616
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 2 cut(s) 1402, 2917
HindII GTYRAC 2 cut(s) 1402, 2917
HindIII AAGCTT 2 cut(s) 2099, 2950
HpaII CCGG 5 cut(s) 356, 363, 702, 1698, 2253
HphI GGTGA 7 cut(s) 57, 370, 1393, 1583, 1754, 2267, 2642
Hpy166II GTNNAC 7 cut(s) 763, 1288, 1373, 1384, 1402, 1782, 2917
Hpy8I GTNNAC 7 cut(s) 763, 1288, 1373, 1384, 1402, 1782, 2917
Hpy99I CGWCG 3 cut(s) 44, 164, 241
HpyCH4III ACNGT 4 cut(s) 331, 456, 2083, 2914
HpyCH4IV ACGT 2 cut(s) 609, 2602
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 181, 559, 600, 613, 2053
HpySE526I ACGT 2 cut(s) 609, 2602
HspAI GCGC 1 cut(s) 614
Ksp22I TGATCA 1 cut(s) 966
LmnI GCTCC 3 cut(s) 172, 512, 1006
Lsp1109I GCAGC 7 cut(s) 581, 938, 1056, 1129, 2668, 2866, 2869
LweI GCATC 7 cut(s) 621, 928, 956, 1627, 2505, 2710, 2800
MaeI CTAG 2 cut(s) 2205, 2984
MaeII ACGT 2 cut(s) 609, 2602
MaeIII GTNAC 7 cut(s) 358, 450, 832, 1717, 2005, 2603, 2924
MbiI CCGCTC 1 cut(s) 409
MfeI CAATTG 1 cut(s) 1893
MflI RGATCY 2 cut(s) 1665, 2369
MhlI GDGCHC 3 cut(s) 83, 509, 1861
MlsI TGGCCA 2 cut(s) 32, 828
MluNI TGGCCA 2 cut(s) 32, 828
MlyI GAGTC 4 cut(s) 203, 748, 1120, 2867
MmeI TCCRAC 1 cut(s) 2933
Mox20I TGGCCA 2 cut(s) 32, 828
MroXI GAANNNNTTC 1 cut(s) 2496
MscI TGGCCA 2 cut(s) 32, 828
MseI TTAA 8 cut(s) 873, 1170, 1500, 1511, 2093, 2361, 2420, 2795
MslI CAYNNNNRTG 1 cut(s) 2706
Msp20I TGGCCA 2 cut(s) 32, 828
MspA1I CMGCKG 2 cut(s) 54, 2681
MspCI CTTAAG 1 cut(s) 2419
MspI CCGG 5 cut(s) 356, 363, 702, 1698, 2253
MunI CAATTG 1 cut(s) 1893
Mva1269I GAATGC 1 cut(s) 2481
MvnI CGCG 3 cut(s) 247, 714, 1790
MwoI GCNNNNNNNGC 6 cut(s) 21, 181, 559, 600, 613, 2053
NciI CCSGG 1 cut(s) 364
NcoI CCATGG 3 cut(s) 1098, 2434, 2694
NlaIV GGNNCC 5 cut(s) 1315, 1422, 1759, 2181, 2692
NmuCI GTSAC 5 cut(s) 358, 450, 2005, 2603, 2924
NspI RCATGY 3 cut(s) 553, 2289, 2722
NspV TTCGAA 5 cut(s) 307, 2500, 2567, 2881, 2947
PagI TCATGA 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 809
PctI GAATGC 1 cut(s) 2481
PdmI GAANNNNTTC 1 cut(s) 2496
PflMI CCANNNNNTGG 1 cut(s) 2219
PinAI ACCGGT 2 cut(s) 1697, 2252
PleI GAGTC 4 cut(s) 203, 748, 1120, 2867
PpsI GAGTC 4 cut(s) 203, 748, 1120, 2867
PpuMI RGGWCCY 1 cut(s) 1314
PsiI TTATAA 1 cut(s) 2615
Psp124BI GAGCTC 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 1314
PspEI GGTNACC 1 cut(s) 358
PspN4I GGNNCC 5 cut(s) 1315, 1422, 1759, 2181, 2692
PspPI GGNCC 5 cut(s) 257, 792, 1314, 2623, 2995
PspPPI RGGWCCY 1 cut(s) 1314
PstI CTGCAG 3 cut(s) 1394, 1726, 2686
PstNI CAGNNNCTG 2 cut(s) 679, 2780
PsuI RGATCY 2 cut(s) 1665, 2369
PvuII CAGCTG 1 cut(s) 2681
RsaI GTAC 5 cut(s) 1971, 1993, 2041, 2576, 2665
RsaNI GTAC 5 cut(s) 1970, 1992, 2040, 2575, 2664
RseI CAYNNNNRTG 1 cut(s) 2706
SacI GAGCTC 1 cut(s) 509
SaqAI TTAA 8 cut(s) 873, 1170, 1500, 1511, 2093, 2361, 2420, 2795
Sau96I GGNCC 5 cut(s) 257, 792, 1314, 2623, 2995
ScaI AGTACT 2 cut(s) 2041, 2665
SchI GAGTC 4 cut(s) 203, 748, 1120, 2867
SduI GDGCHC 3 cut(s) 83, 509, 1861
SfaNI GCATC 7 cut(s) 621, 928, 956, 1627, 2505, 2710, 2800
SfcI CTRYAG 7 cut(s) 1284, 1390, 1722, 2407, 2655, 2676, 2682
SfuI TTCGAA 5 cut(s) 307, 2500, 2567, 2881, 2947
SinI GGWCC 3 cut(s) 792, 1314, 2623
SmiMI CAYNNNNRTG 1 cut(s) 2706
SmlI CTYRAG 6 cut(s) 152, 617, 1441, 1466, 2419, 2863
SmoI CTYRAG 6 cut(s) 152, 617, 1441, 1466, 2419, 2863
SspI AATATT 2 cut(s) 1435, 1480
SspMI CTAG 2 cut(s) 2205, 2984
SstI GAGCTC 1 cut(s) 509
StyI CCWWGG 6 cut(s) 1098, 1339, 2434, 2619, 2626, 2694
TaaI ACNGT 4 cut(s) 331, 456, 2083, 2914
TaiI ACGT 2 cut(s) 612, 2605
TatI WGTACW 2 cut(s) 2039, 2663
TauI GCSGC 5 cut(s) 54, 62, 151, 409, 1772
Tru1I TTAA 8 cut(s) 873, 1170, 1500, 1511, 2093, 2361, 2420, 2795
Tru9I TTAA 8 cut(s) 873, 1170, 1500, 1511, 2093, 2361, 2420, 2795
TscAI CASTG 6 cut(s) 172, 379, 459, 1242, 1599, 1833
TseFI GTSAC 5 cut(s) 358, 450, 2005, 2603, 2924
TseI GCWGC 7 cut(s) 594, 926, 1044, 1117, 2681, 2854, 2857
Tsp45I GTSAC 5 cut(s) 358, 450, 2005, 2603, 2924
TspGWI ACGGA 3 cut(s) 225, 717, 2562
TspRI CASTG 6 cut(s) 172, 379, 459, 1242, 1599, 1833
Van91I CCANNNNNTGG 1 cut(s) 2219
Vha464I CTTAAG 1 cut(s) 2419
VpaK11BI GGWCC 3 cut(s) 792, 1314, 2623
XapI RAATTY 4 cut(s) 300, 972, 1618, 2493
XceI RCATGY 3 cut(s) 553, 2289, 2722
XmiI GTMKAC 2 cut(s) 1287, 1781
XmnI GAANNNNTTC 1 cut(s) 2496
XspI CTAG 2 cut(s) 2205, 2984
ZrmI AGTACT 2 cut(s) 2041, 2665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.