Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0010084

Physical Location & Seq

Genomic Coordinates Reverse (-)

57104694 .. 57108081

Gene Structure 3388 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G032860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2469 bp

ATGGTGGCATGTTGCCTCATGTGCCAGGTGGCCATCGCCGTCGATATGAGCCGCTGTGCCGCTTCACCTCCCAATCTGTGCCCCCTCCAGTTCTCAGGTAGCCCCGCGAAGCTATTCGGATTCGGGTTCGACATTCAGAAGAAGGACCGGCTCAAGAGGCGGCTCAAGTTCGTCGTCAGTGCGGAGCTGTCCAAGCCATTCTCTCTCGGCTTCGGCTTGGACTCTCAGGGGTATCAGCTTGACGATCCGTCGCAGTCGCGGAGGATTGGCCCTGTTCCTGGGGATATTGCAGAAATTGAAGCCTATTGTAGAATTTTTCGAAGCGCTGAACGACTGCATACTGCATTGATGGAGACATTGTGCAATCCGGTGACCGGGGTGTGCAGTGTTTATTATGACTTCCCATCGGAGGAAAAGCCGCTCTTGGAGGATAAGATTGTGTCTGTGATTGGTTGCATGATGTCACTGTTGAACAAAGCAAGGGAGGATGTGCTTTCCGGAAGATCAGCCATTATGAGCTCCTTTCGAGGAGTTGTGGATGTCGCTCTTCCTCCACTTGCTGTTTTCAGGAGTGAGCTGAAGAGGTGTTGCGAGAGCTTGCACGTTGCGCTTGAGGACTGGTTGATGCCTGGGGATGACCAGAGCTTGGATGTGTGGAGGAAACTTCAGAGGCTCAAGAATGTGTGTTATGATTCCGGTTTTCCACGCCAGGAGGATTATCCGTGCAACACGCTTTTTGCTAATTGGACTCCTGTTTACTTATCCTCTTCTAAAGAGGATGATGTTAGGTCCCAAGATTCAGAGGTAGCGTTTTGGAGGGGTGGCCAGGTAACAGAAGAAGGTCTGGACTGGTTACTGGAGAAGGGATTTAAAACTCTTGTTGATCTTAGGGCAGAGATTGTAAAAGACAATATTTATCAGGCAGCCGTAGATGATGCTATTGCATCAGGGAAGGTGAAATTGATCAGAATTCCAGTTGAAGTTGGGACTGCACCTTCAATGGAGCAGGTTGAGAAATTCGCAAGTTTGGTTTCAGATTGCAGCAAAAACCCAATCTATCTTCATAGCAAGGAAGGTCTCTGGAGAACATCTGCCATGGTTTCCAGATGGAGGCAGCACTTGACTCGCCGTGCTGTTCAGGTTGTCTCCAAGCAGTCAGTTTCTCTTAATGGAGAGGTTGAAAAACCCTCAACTATGCAGAAAAGTTCCCTCCTTGAAAAGAAGAATGAATCACTGGACACAATTCATGGTTCAAATGGGTTGCTACAAAAAAATGATTCTGTAGACAGGGATGAAGCAAATCTAAATGGGACCTGCAATGGCCTTATATCTATCCAAGGTTTGACATCAGTTGAATCAGATGAAAGTGGGGAAAAACCTTTGGTGAACTTCTGCAGGGAAGTTGACCCCCTGAATGCTCAGGTTCCTCCTTGCAATATTTTCTCAAGAAAAGAAATGTCCAGGTTTCTTGAGAGAAAAAATATTGCACCACTCACTTATTTTAATTATCAGTTAAATAGGTTGAAGGTATTACCAATTTCCAAATATATGAATACTAAGATAATGTGGAGAGGTGAGATTGTTGATACTGATTCAGTGCGGGAGGTTGTGGAGGCAGAAAATTCCAATGGGATACCTGATGCAGAACATCTGCTTCCAGAGTTCCAGATCTCTGCTTCTGGCAATGGGAAGTATTTGACCGGTGTTGGTAATGGTTCTGTAACTGCAGTCGTAAATGGATATGGTGAAGGGGAGAAAGGGTCCTTGTTAGCCACTAATTTATCTACTAACGCGAGCAACAATCAATCTGAGAGTGTCTTATCTAAAGCAGTGAAGGAGGAAAGGAAGAGTAATGGTAGAGCCCATTTAGTTTCAAGTGATGATGAATTGGGATCAATTGAAGGAGATATGTGTGCTTCTGCAACTGGGGTTGTAAGGGTGCAGTCAAGAAAGAAAGCAGAGATGTTTTTAGTACGAACAGATGGATTCTCTTGTACCCGAGAAAAAGTGACAGAATCCTCCCTGGCCTTCACTCATCCAAGTACTCAGCAACAAATGCTTATGTGGAAATCTACACCAAAGACAGTATTAGTGTTAAAGAAGCTTGGGCATGAGCTTATGGAACAAGCTAAAGAGGTTGTCTCCTTCTTGTATTACCAAGAGAAAATGAATGTTCTGGTGGAACCTGACGTGCATGATGTATTTGCTCGAATCCCAGGTTTTGGATTTGTTCAGACCTTCTACACCCAAGATACCGGTGATCTGCATGAGAGGGTTGATTTTGTGGCATGTCTGGGAGGGGATGGGGTAATACTCCATGCTTCAAATCTATTCAAAGGTGCTGTTCCCCCCATCGTATCATTTAATCTTGGATCTCTTGGATTTTTGACTTCCCATACTTTTGATGACTACAGGCAGGACTTAAGACAGGTTATCCATGGAAATAACACGAGTGATGGTTTTATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00760 ko01100 map00760 map01100

Pfam Domains

Protein Families

NAD_kinase

Protein Analysis

822

Amino Acids

91.18

Weight (kDa)

5.71

Isoelectric Point (pI)

39.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PTP-NADK	PF22741	241 - 390	6.6e-63	DSP-PTPase phosphatase fused to NAD+ Kinase
Tc-R-P	PF22785	276 - 370	1.2e-10	Polymorphic toxin system, DSP-PTPase phosphatase
NAD_kinase	PF01513	704 - 814	3.5e-15	ATP-NAD kinase N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010084)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21640 AT1G21640
fragaria_vesca	FvH4_2g27820 FvH4_2g27820
malus_domestica	MD08G1160800.v1.1
prunus_persica	Prupe.1G489900_v2.0.a1 Prupe.1G489900_v2.0.a1 Prupe.1G489900_v2.0.a1
pyrus_communis	pycom348g00070
rosa_chinensis	RchiOBHm_Chr6g0296671
rosa_laevigata	RLG00000011671
rosa_multiflora	Rmu_sc0001741.1_g000009
rosa_roxburghii	Rroxscaffold_7G00171270
rosa_rugosa	Rorug06G0264100 Rorug06G0264100
rosa_samantha	Rh6AG375900 Rh6BG384200 Rh6CG389300 Rh6DG376700
rosa_wichuraiana	Rw6G032770 Rw6G032860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	997, 1322
AccB7I	CCANNNNNTGG	2 cut(s)	646, 2222
AccBSI	CCGCTC	1 cut(s)	421
AccI	GTMKAC	1 cut(s)	1284
AccII	CGCG	3 cut(s)	107, 259, 1793
AccIII	TCCGGA	1 cut(s)	497
AciI	CCGC	8 cut(s)	52, 60, 105, 160, 182, 259, 419, 1600
AclWI	GGATC	3 cut(s)	239, 1900, 2380
AcoI	YGGCCR	2 cut(s)	30, 823
AcsI	RAATTY	4 cut(s)	312, 969, 1016, 1621
AcuI	CTGAAG	2 cut(s)	599, 650
AfaI	GTAC	3 cut(s)	1974, 1996, 2044
AfeI	AGCGCT	1 cut(s)	325
AfiI	CCNNNNNNNGG	6 cut(s)	278, 374, 409, 646, 1110, 2222
AflII	CTTAAG	1 cut(s)	2422
AgeI	ACCGGT	2 cut(s)	1700, 2255
AhdI	GACNNNNNGTC	1 cut(s)	247
AjiI	CACGTC	1 cut(s)	2191
AjnI	CCWGG	8 cut(s)	24, 277, 628, 708, 825, 1460, 2022, 2215
Alw21I	GWGCWC	1 cut(s)	521
Alw26I	GTCTC	4 cut(s)	347, 1082, 1150, 2146
AlwI	GGATC	3 cut(s)	239, 1900, 2380
Ama87I	CYCGRG	1 cut(s)	1998
Aor13HI	TCCGGA	1 cut(s)	497
Aor51HI	AGCGCT	1 cut(s)	325
AoxI	GGCC	5 cut(s)	30, 268, 823, 1321, 2025
ApeKI	GCWGC	3 cut(s)	923, 1041, 1114
ApoI	RAATTY	4 cut(s)	312, 969, 1016, 1621
AsiGI	ACCGGT	2 cut(s)	1700, 2255
Asp700I	GAANNNNTTC	1 cut(s)	113
AspLEI	GCGC	2 cut(s)	326, 610
AspS9I	GGNCC	5 cut(s)	145, 269, 789, 1311, 1761
AsuC2I	CCSGG	1 cut(s)	376
AsuHPI	GGTGA	7 cut(s)	57, 382, 967, 1396, 1586, 1757, 2270
AsuII	TTCGAA	1 cut(s)	319
AvaI	CYCGRG	1 cut(s)	1998
AvaII	GGWCC	4 cut(s)	145, 789, 1311, 1761
BaeGI	GKGCMC	1 cut(s)	83
BalI	TGGCCA	2 cut(s)	32, 825
BanII	GRGCYC	2 cut(s)	521, 1864
BauI	CACGAG	1 cut(s)	2449
Bbv12I	GWGCWC	1 cut(s)	521
BbvI	GCAGC	3 cut(s)	935, 1053, 1126
BccI	CCATC	8 cut(s)	41, 343, 412, 1101, 1976, 2297, 2360, 2450
BceAI	ACGGC	3 cut(s)	23, 911, 1113
BcgI	CGANNNNNNTGC	2 cut(s)	1106, 1140
BciT130I	CCWGG	8 cut(s)	26, 279, 630, 710, 827, 1462, 2024, 2217
BciVI	GTATCC	1 cut(s)	1626
BclI	TGATCA	1 cut(s)	963
BcnI	CCSGG	1 cut(s)	376
BcoDI	GTCTC	4 cut(s)	347, 1082, 1150, 2146
BfmI	CTRYAG	4 cut(s)	1281, 1393, 1725, 2410
BfoI	RGCGCY	1 cut(s)	327
BfrI	CTTAAG	1 cut(s)	2422
BfuAI	ACCTGC	2 cut(s)	997, 1322
BfuI	GTATCC	1 cut(s)	1626
BglII	AGATCT	1 cut(s)	1668
BisI	GCNGC	7 cut(s)	52, 60, 161, 419, 924, 1042, 1115
BlsI	GCNGC	7 cut(s)	53, 61, 162, 420, 925, 1043, 1116
BmcAI	AGTACT	1 cut(s)	2044
Bme1390I	CCNGG	9 cut(s)	26, 279, 376, 630, 710, 827, 1462, 2024, 2217
Bme18I	GGWCC	4 cut(s)	145, 789, 1311, 1761
BmeRI	GACNNNNNGTC	1 cut(s)	247
BmeT110I	CYCGRG	1 cut(s)	1998
BmgBI	CACGTC	1 cut(s)	2191
BmgT120I	GGNCC	5 cut(s)	145, 269, 789, 1311, 1761
BmiI	GGNNCC	5 cut(s)	791, 1312, 1425, 1762, 2184
BmrFI	CCNGG	9 cut(s)	26, 279, 376, 630, 710, 827, 1462, 2024, 2217
BmrI	ACTGGG	1 cut(s)	1935
BmsI	GCATC	4 cut(s)	615, 925, 953, 1630
BmuI	ACTGGG	1 cut(s)	1935
BplI	GAGNNNNNCTC	2 cut(s)	2126, 2158
BpmI	CTGGAG	3 cut(s)	71, 878, 1102
Bpu10I	CCTNAGC	1 cut(s)	1419
Bpu14I	TTCGAA	1 cut(s)	319
BpuEI	CTTGAG	6 cut(s)	137, 149, 632, 659, 1429, 1490
BpuMI	CCSGG	1 cut(s)	376
BsaI	GGTCTC	1 cut(s)	1082
BsaJI	CCNNGG	8 cut(s)	278, 375, 629, 1095, 1336, 2022, 2215, 2437
BsaWI	WCCGGW	5 cut(s)	367, 497, 695, 1700, 2255
BsaXI	ACNNNNNCTCC	4 cut(s)	344, 374, 1745, 1775
Bsc4I	CCNNNNNNNGG	6 cut(s)	278, 374, 409, 646, 1110, 2222
Bse118I	RCCGGY	3 cut(s)	147, 1700, 2255
Bse1I	ACTGG	7 cut(s)	88, 623, 854, 861, 974, 1239, 1930
Bse3DI	GCAATG	2 cut(s)	1324, 1690
BseAI	TCCGGA	1 cut(s)	497
BseBI	CCWGG	8 cut(s)	26, 279, 630, 710, 827, 1462, 2024, 2217
BseDI	CCNNGG	8 cut(s)	278, 375, 629, 1095, 1336, 2022, 2215, 2437
BseGI	GGATG	8 cut(s)	493, 544, 640, 655, 784, 1297, 2035, 2308
BseLI	CCNNNNNNNGG	6 cut(s)	278, 374, 409, 646, 1110, 2222
BseMI	GCAATG	2 cut(s)	1324, 1690
BseMII	CTCAG	5 cut(s)	108, 239, 1433, 1800, 2060
BseNI	ACTGG	7 cut(s)	88, 623, 854, 861, 974, 1239, 1930
BseRI	GAGGAG	1 cut(s)	543
BseSI	GKGCMC	1 cut(s)	83
BseXI	GCAGC	3 cut(s)	935, 1053, 1126
BsgI	GTGCAG	3 cut(s)	403, 975, 1961
Bsh1236I	CGCG	3 cut(s)	107, 259, 1793
BshFI	GGCC	5 cut(s)	32, 270, 825, 1323, 2027
BshTI	ACCGGT	2 cut(s)	1700, 2255
BsiHKAI	GWGCWC	1 cut(s)	521
BsiHKCI	CYCGRG	1 cut(s)	1998
BsiSI	CCGG	7 cut(s)	148, 368, 375, 498, 696, 1701, 2256
BslFI	GGGAC	3 cut(s)	775, 1000, 1324
BslI	CCNNNNNNNGG	6 cut(s)	278, 374, 409, 646, 1110, 2222
BsmAI	GTCTC	4 cut(s)	347, 1082, 1150, 2146
BsmFI	GGGAC	3 cut(s)	775, 1000, 1324
BsmI	GAATGC	1 cut(s)	1420
BsnI	GGCC	5 cut(s)	32, 270, 825, 1323, 2027
Bso31I	GGTCTC	1 cut(s)	1082
BsoBI	CYCGRG	1 cut(s)	1998
Bsp119I	TTCGAA	1 cut(s)	319
Bsp1286I	GDGCHC	3 cut(s)	83, 521, 1864
Bsp13I	TCCGGA	1 cut(s)	497
Bsp143I	GATC	8 cut(s)	244, 503, 883, 963, 1668, 1892, 2260, 2372
Bsp19I	CCATGG	2 cut(s)	1095, 2437
BspACI	CCGC	8 cut(s)	52, 60, 105, 160, 182, 259, 419, 1600
BspANI	GGCC	5 cut(s)	32, 270, 825, 1323, 2027
BspCNI	CTCAG	5 cut(s)	107, 238, 1432, 1801, 2059
BspEI	TCCGGA	1 cut(s)	497
BspFNI	CGCG	3 cut(s)	107, 259, 1793
BspLI	GGNNCC	5 cut(s)	791, 1312, 1425, 1762, 2184
BspMAI	CTGCAG	2 cut(s)	1397, 1729
BspMI	ACCTGC	2 cut(s)	997, 1322
BspPI	GGATC	3 cut(s)	239, 1900, 2380
BspQI	GCTCTTC	1 cut(s)	552
BspT104I	TTCGAA	1 cut(s)	319
BspTI	CTTAAG	1 cut(s)	2422
BspTNI	GGTCTC	1 cut(s)	1082
BsrBI	CCGCTC	1 cut(s)	421
BsrDI	GCAATG	2 cut(s)	1324, 1690
BsrFI	RCCGGY	3 cut(s)	147, 1700, 2255
BsrI	ACTGG	7 cut(s)	88, 623, 854, 861, 974, 1239, 1930
BssAI	RCCGGY	3 cut(s)	147, 1700, 2255
BssECI	CCNNGG	8 cut(s)	278, 375, 629, 1095, 1336, 2022, 2215, 2437
BssMI	GATC	8 cut(s)	244, 503, 883, 963, 1668, 1892, 2260, 2372
BssSI	CACGAG	1 cut(s)	2449
BssT1I	CCWWGG	3 cut(s)	1095, 1336, 2437
Bst2BI	CACGAG	1 cut(s)	2449
Bst2UI	CCWGG	8 cut(s)	26, 279, 630, 710, 827, 1462, 2024, 2217
Bst4CI	ACNGT	2 cut(s)	468, 2086
Bst6I	CTCTTC	4 cut(s)	552, 575, 772, 1841
BstAFI	CTTAAG	1 cut(s)	2422
BstAPI	GCANNNNNTGC	1 cut(s)	2056
BstBI	TTCGAA	1 cut(s)	319
BstC8I	GCNNGC	2 cut(s)	599, 1795
BstDEI	CTNAG	7 cut(s)	94, 225, 887, 1419, 1557, 1809, 2046
BstDSI	CCRYGG	2 cut(s)	1095, 2437
BstEII	GGTNACC	1 cut(s)	370
BstF5I	GGATG	8 cut(s)	493, 544, 640, 655, 784, 1297, 2035, 2308
BstFNI	CGCG	3 cut(s)	107, 259, 1793
BstH2I	RGCGCY	1 cut(s)	327
BstHHI	GCGC	2 cut(s)	326, 610
BstKTI	GATC	8 cut(s)	247, 506, 886, 966, 1671, 1895, 2263, 2375
BstMAI	GTCTC	4 cut(s)	347, 1082, 1150, 2146
BstMBI	GATC	8 cut(s)	244, 503, 883, 963, 1668, 1892, 2260, 2372
BstMWI	GCNNNNNNNGC	5 cut(s)	21, 157, 193, 607, 2056
BstNI	CCWGG	8 cut(s)	26, 279, 630, 710, 827, 1462, 2024, 2217
BstNSI	RCATGY	2 cut(s)	12, 2292
BstPI	GGTNACC	1 cut(s)	370
BstSCI	CCNGG	9 cut(s)	24, 277, 374, 628, 708, 825, 1460, 2022, 2215
BstSFI	CTRYAG	4 cut(s)	1281, 1393, 1725, 2410
BstSLI	GKGCMC	1 cut(s)	83
BstUI	CGCG	3 cut(s)	107, 259, 1793
BstV1I	GCAGC	3 cut(s)	935, 1053, 1126
BstX2I	RGATCY	2 cut(s)	1668, 2372
BstYI	RGATCY	2 cut(s)	1668, 2372
BsuI	GTATCC	1 cut(s)	1626
BsuRI	GGCC	5 cut(s)	32, 270, 825, 1323, 2027
BtgI	CCRYGG	2 cut(s)	1095, 2437
BtgZI	GCGATG	1 cut(s)	19
BtrI	CACGTC	1 cut(s)	2191
BtsCI	GGATG	8 cut(s)	493, 544, 640, 655, 784, 1297, 2035, 2308
BtsI	GCAGTG	2 cut(s)	391, 1836
BtsIMutI	CAGTG	6 cut(s)	184, 391, 464, 1232, 1602, 1836
BveI	ACCTGC	2 cut(s)	997, 1322
Cac8I	GCNNGC	2 cut(s)	599, 1795
CfoI	GCGC	2 cut(s)	326, 610
Cfr10I	RCCGGY	3 cut(s)	147, 1700, 2255
Cfr13I	GGNCC	5 cut(s)	145, 269, 789, 1311, 1761
Csp6I	GTAC	3 cut(s)	1973, 1995, 2043
CspAI	ACCGGT	2 cut(s)	1700, 2255
CviQI	GTAC	3 cut(s)	1973, 1995, 2043
DdeI	CTNAG	7 cut(s)	94, 225, 887, 1419, 1557, 1809, 2046
DpnI	GATC	8 cut(s)	246, 505, 885, 965, 1670, 1894, 2262, 2374
DpnII	GATC	8 cut(s)	244, 503, 883, 963, 1668, 1892, 2260, 2372
DraI	TTTAAA	1 cut(s)	871
DriI	GACNNNNNGTC	1 cut(s)	247
EaeI	YGGCCR	2 cut(s)	30, 823
Eam1104I	CTCTTC	4 cut(s)	552, 575, 772, 1841
Eam1105I	GACNNNNNGTC	1 cut(s)	247
EarI	CTCTTC	4 cut(s)	552, 575, 772, 1841
Ecl136II	GAGCTC	1 cut(s)	519
Eco130I	CCWWGG	3 cut(s)	1095, 1336, 2437
Eco24I	GRGCYC	2 cut(s)	521, 1864
Eco31I	GGTCTC	1 cut(s)	1082
Eco47I	GGWCC	4 cut(s)	145, 789, 1311, 1761
Eco47III	AGCGCT	1 cut(s)	325
Eco53kI	GAGCTC	1 cut(s)	519
Eco57I	CTGAAG	2 cut(s)	599, 650
Eco88I	CYCGRG	1 cut(s)	1998
Eco91I	GGTNACC	1 cut(s)	370
EcoICRI	GAGCTC	1 cut(s)	519
EcoO109I	RGGNCCY	3 cut(s)	789, 1311, 1761
EcoO65I	GGTNACC	1 cut(s)	370
EcoRI	GAATTC	1 cut(s)	969
EcoRII	CCWGG	8 cut(s)	24, 277, 628, 708, 825, 1460, 2022, 2215
EcoT14I	CCWWGG	3 cut(s)	1095, 1336, 2437
EcoT38I	GRGCYC	2 cut(s)	521, 1864
ErhI	CCWWGG	3 cut(s)	1095, 1336, 2437
FalI	AAGNNNNNCTT	2 cut(s)	407, 439
FaqI	GGGAC	3 cut(s)	775, 1000, 1324
FauI	CCCGC	2 cut(s)	112, 1593
FbaI	TGATCA	1 cut(s)	963
FblI	GTMKAC	1 cut(s)	1284
Fnu4HI	GCNGC	7 cut(s)	52, 60, 161, 419, 924, 1042, 1115
FokI	GGATG	8 cut(s)	500, 551, 647, 662, 791, 1304, 2022, 2315
FriOI	GRGCYC	2 cut(s)	521, 1864
Fsp4HI	GCNGC	7 cut(s)	52, 60, 161, 419, 924, 1042, 1115
GlaI	GCGC	2 cut(s)	325, 609
GluI	GCNGC	7 cut(s)	52, 60, 161, 419, 924, 1042, 1115
GsuI	CTGGAG	3 cut(s)	71, 878, 1102
HaeII	RGCGCY	1 cut(s)	327
HaeIII	GGCC	5 cut(s)	32, 270, 825, 1323, 2027
HapII	CCGG	7 cut(s)	148, 368, 375, 498, 696, 1701, 2256
HhaI	GCGC	2 cut(s)	326, 610
Hin6I	GCGC	2 cut(s)	324, 608
HinP1I	GCGC	2 cut(s)	324, 608
HincII	GTYRAC	1 cut(s)	1405
HindII	GTYRAC	1 cut(s)	1405
HindIII	AAGCTT	1 cut(s)	2102
HpaII	CCGG	7 cut(s)	148, 368, 375, 498, 696, 1701, 2256
HphI	GGTGA	7 cut(s)	57, 382, 967, 1396, 1586, 1757, 2270
Hpy166II	GTNNAC	4 cut(s)	757, 1285, 1387, 1405
Hpy8I	GTNNAC	4 cut(s)	757, 1285, 1387, 1405
Hpy99I	CGWCG	3 cut(s)	44, 176, 253
HpyCH4III	ACNGT	2 cut(s)	468, 2086
HpyCH4IV	ACGT	2 cut(s)	603, 2190
HpyF10VI	GCNNNNNNNGC	5 cut(s)	21, 157, 193, 607, 2056
HpyF3I	CTNAG	7 cut(s)	94, 225, 887, 1419, 1557, 1809, 2046
HpySE526I	ACGT	2 cut(s)	603, 2190
HspAI	GCGC	2 cut(s)	324, 608
Kpn2I	TCCGGA	1 cut(s)	497
Ksp22I	TGATCA	1 cut(s)	963
Kzo9I	GATC	8 cut(s)	244, 503, 883, 963, 1668, 1892, 2260, 2372
LguI	GCTCTTC	1 cut(s)	552
LmnI	GCTCC	3 cut(s)	184, 524, 1003
Lsp1109I	GCAGC	3 cut(s)	935, 1053, 1126
LweI	GCATC	4 cut(s)	615, 925, 953, 1630
MaeII	ACGT	2 cut(s)	603, 2190
MaeIII	GTNAC	6 cut(s)	370, 462, 829, 852, 1720, 2008
MalI	GATC	8 cut(s)	246, 505, 885, 965, 1670, 1894, 2262, 2374
MbiI	CCGCTC	1 cut(s)	421
MboI	GATC	8 cut(s)	244, 503, 883, 963, 1668, 1892, 2260, 2372
MboII	GAAGA	9 cut(s)	151, 513, 539, 592, 759, 848, 1052, 1234, 1858
MfeI	CAATTG	1 cut(s)	1896
MflI	RGATCY	2 cut(s)	1668, 2372
MhlI	GDGCHC	3 cut(s)	83, 521, 1864
MlsI	TGGCCA	2 cut(s)	32, 825
MluNI	TGGCCA	2 cut(s)	32, 825
MlyI	GAGTC	3 cut(s)	215, 742, 1117
Mox20I	TGGCCA	2 cut(s)	32, 825
MroI	TCCGGA	1 cut(s)	497
MroXI	GAANNNNTTC	1 cut(s)	113
MscI	TGGCCA	2 cut(s)	32, 825
MseI	TTAA	7 cut(s)	870, 1167, 1503, 1514, 2096, 2364, 2423
Msp20I	TGGCCA	2 cut(s)	32, 825
MspA1I	CMGCKG	1 cut(s)	54
MspCI	CTTAAG	1 cut(s)	2422
MspI	CCGG	7 cut(s)	148, 368, 375, 498, 696, 1701, 2256
MspR9I	CCNGG	9 cut(s)	26, 279, 376, 630, 710, 827, 1462, 2024, 2217
MunI	CAATTG	1 cut(s)	1896
Mva1269I	GAATGC	1 cut(s)	1420
MvaI	CCWGG	8 cut(s)	26, 279, 630, 710, 827, 1462, 2024, 2217
MvnI	CGCG	3 cut(s)	107, 259, 1793
MwoI	GCNNNNNNNGC	5 cut(s)	21, 157, 193, 607, 2056
NciI	CCSGG	1 cut(s)	376
NcoI	CCATGG	2 cut(s)	1095, 2437
NdeII	GATC	8 cut(s)	244, 503, 883, 963, 1668, 1892, 2260, 2372
NlaIV	GGNNCC	5 cut(s)	791, 1312, 1425, 1762, 2184
NmeAIII	GCCGAG	1 cut(s)	186
NmuCI	GTSAC	3 cut(s)	370, 462, 2008
NspI	RCATGY	2 cut(s)	12, 2292
NspV	TTCGAA	1 cut(s)	319
PciSI	GCTCTTC	1 cut(s)	552
PctI	GAATGC	1 cut(s)	1420
PdmI	GAANNNNTTC	1 cut(s)	113
PflMI	CCANNNNNTGG	2 cut(s)	646, 2222
PinAI	ACCGGT	2 cut(s)	1700, 2255
PkrI	GCNGC	7 cut(s)	53, 61, 162, 420, 925, 1043, 1116
PleI	GAGTC	3 cut(s)	215, 742, 1117
PpsI	GAGTC	3 cut(s)	215, 742, 1117
PpuMI	RGGWCCY	3 cut(s)	789, 1311, 1761
Psp124BI	GAGCTC	1 cut(s)	521
Psp5II	RGGWCCY	3 cut(s)	789, 1311, 1761
Psp6I	CCWGG	8 cut(s)	24, 277, 628, 708, 825, 1460, 2022, 2215
PspEI	GGTNACC	1 cut(s)	370
PspGI	CCWGG	8 cut(s)	24, 277, 628, 708, 825, 1460, 2022, 2215
PspN4I	GGNNCC	5 cut(s)	791, 1312, 1425, 1762, 2184
PspPI	GGNCC	5 cut(s)	145, 269, 789, 1311, 1761
PspPPI	RGGWCCY	3 cut(s)	789, 1311, 1761
PstI	CTGCAG	2 cut(s)	1397, 1729
PsuI	RGATCY	2 cut(s)	1668, 2372
RsaI	GTAC	3 cut(s)	1974, 1996, 2044
RsaNI	GTAC	3 cut(s)	1973, 1995, 2043
SacI	GAGCTC	1 cut(s)	521
SapI	GCTCTTC	1 cut(s)	552
SaqAI	TTAA	7 cut(s)	870, 1167, 1503, 1514, 2096, 2364, 2423
SatI	GCNGC	7 cut(s)	52, 60, 161, 419, 924, 1042, 1115
Sau3AI	GATC	8 cut(s)	244, 503, 883, 963, 1668, 1892, 2260, 2372
Sau96I	GGNCC	5 cut(s)	145, 269, 789, 1311, 1761
ScaI	AGTACT	1 cut(s)	2044
SchI	GAGTC	3 cut(s)	215, 742, 1117
ScrFI	CCNGG	9 cut(s)	26, 279, 376, 630, 710, 827, 1462, 2024, 2217
SduI	GDGCHC	3 cut(s)	83, 521, 1864
SfaNI	GCATC	4 cut(s)	615, 925, 953, 1630
SfcI	CTRYAG	4 cut(s)	1281, 1393, 1725, 2410
SfuI	TTCGAA	1 cut(s)	319
SinI	GGWCC	4 cut(s)	145, 789, 1311, 1761
SmlI	CTYRAG	7 cut(s)	152, 164, 611, 674, 1444, 1469, 2422
SmoI	CTYRAG	7 cut(s)	152, 164, 611, 674, 1444, 1469, 2422
SsiI	CCGC	8 cut(s)	52, 60, 105, 160, 182, 259, 419, 1600
SspI	AATATT	3 cut(s)	913, 1438, 1483
SstI	GAGCTC	1 cut(s)	521
StyD4I	CCNGG	9 cut(s)	24, 277, 374, 628, 708, 825, 1460, 2022, 2215
StyI	CCWWGG	3 cut(s)	1095, 1336, 2437
TaaI	ACNGT	2 cut(s)	468, 2086
TaiI	ACGT	2 cut(s)	606, 2193
TaqI	TCGA	5 cut(s)	42, 129, 319, 526, 2209
TatI	WGTACW	1 cut(s)	2042
TauI	GCSGC	4 cut(s)	54, 62, 163, 421
Tru1I	TTAA	7 cut(s)	870, 1167, 1503, 1514, 2096, 2364, 2423
Tru9I	TTAA	7 cut(s)	870, 1167, 1503, 1514, 2096, 2364, 2423
TscAI	CASTG	6 cut(s)	184, 391, 471, 1239, 1602, 1836
TseFI	GTSAC	3 cut(s)	370, 462, 2008
TseI	GCWGC	3 cut(s)	923, 1041, 1114
Tsp45I	GTSAC	3 cut(s)	370, 462, 2008
TspDTI	ATGAA	8 cut(s)	1052, 1235, 1242, 1308, 1377, 1565, 1899, 2183
TspGWI	ACGGA	2 cut(s)	237, 711
TspRI	CASTG	6 cut(s)	184, 391, 471, 1239, 1602, 1836
Van91I	CCANNNNNTGG	2 cut(s)	646, 2222
Vha464I	CTTAAG	1 cut(s)	2422
VpaK11BI	GGWCC	4 cut(s)	145, 789, 1311, 1761
XapI	RAATTY	4 cut(s)	312, 969, 1016, 1621
XceI	RCATGY	2 cut(s)	12, 2292
XmiI	GTMKAC	1 cut(s)	1284
XmnI	GAANNNNTTC	1 cut(s)	113
ZrmI	AGTACT	1 cut(s)	2044

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.