RchiOBHm_Chr6g0296671

NAD kinase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010084
Physical Location & Seq
Reverse (-)
58427888 .. 58432689
4802 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26629

Sequence Viewer

Length: 3015 bp
ATGGTGGCATGTTGCCTCATGTGCCAGGTGGCCATCGCCGTCGATATGAGCCGCTGTGCCGCTTCACCTCCCAATCTGTGCCCCCTCCAGTTCTCAGGTAGCCCCGCCAAGCTATTCGGATTCGGGTTCGACATTCAGAAGAAGGACCGGCTCAAGAGGCGGCTCAAGTTCGTCGTCAGTGCGGAGCTGTCCAAGCCATTCTCTCTCAGCTTCGGCTTGGACTCTCAGGGCTATCAGCTTCATGATCCGTCGCAGTCGCGGAGGATTGGCCCTGTTCCTGGGGATATTGCAGAAATTGAAGCCTATTGTAGAATTTTTCGAAGTGCTGAACGACTACATACTGCATTGATGGAGACATTGTGCAATCCGGTGACCGGGGTGTGCAGTGTTTATTATGACTTCCCATCGGAGGAAAAGCCGCTCTTGGAGGATAAGATTGTGTCTGTGATTGGTTGCATGGTGTCACTGTTGAACAAAGCAAGGGAGGATGTGCTTTCGGGAAGATCAGCCATTATGAGCTCCTTTCGAGGAGTTGTGGATGTCGCTGAGGTGGAGGAGGACATGCTTCCTCCACTTGCTGTTTTCAGGAGTGAGCTGAAGAGGTGTTGCGAGAGCTTGCACGTTGCGCTTGAGGACTGGTTGATGCCTGGGGATGAGCAGAGCTTGGATGTGTGGAGGAAACTTCAGAGGCTGAAGAATGTGTGTTATGATTCCGGTTTTCCACGCGAGGAGGATTATCCGTGCAATACGCTTTTTGCTAATTGGACTCCTGTTTACTTATCCTCTTCTAAAGAGGATGTTAGGTCCGAAGATTCAGAGGTAGCGTTTTGGAGGGGTGGCCAGGTAACAGAAGAAGGTCTGGAGTGGTTACTGGAGAAGGGATTTAAAACTCTTGTTGATCTTAGGGCAGAGATTGTAAAAGACAATATTTATCAGGCAGCCGTAGATGATGCTATTGCATCAGGGAAGGTGGAATTGATCAGAATTCCAGTTGAAGTTGGGACTGCACCTTCAATGGAGCAGGTTGAGAAATTCGCAAGTTTGGTTTCAGATTGCAGCAAAAAGCCAATCTATCTTCATAGCAAGGAAGGTCTCTGGAGAACATCTGCCATGCTTTCCAGATGGAGGCAGCACTTGACTCGCCGTGCTGTTCAGTTTGTCTCCAAGCAGTCGGTTTCTCTTAATGGAGAAGTTGGAAAACCCTCAACTATGCAGAAAAGTTCCCTCCTTGAAAAGAAGAATGAGTCACTGGACACAATTCATGGTTCAAATGGGGTGCTACAAAAAAATGATTCTGTAGACAGGGATGAAGCAAATCTAAATGGGACCTGCAATAGCCTTATATCTATCCAAGGTTTGAGATCAGTTGAATCAGATGAAAGTGAACCTTTGGTGAACTTCTGCAGGGAAGTTGACCCCCTGAATGCTCAGGTTCCTCCTTGCAATATTTTCTCAAGAAAAGAAATGTCCAGGTTTCTTGAGAGAAAAAATATTGCACCACTCACTTATTTTAATTATCAGTTAAATAGGTTGAAGGTATTACCAATTTCCAGATATATGAATACTAAGATAATGTGGAGAGGTGAGATTGTTGATACTGATTCAGTGCGGGAGGTTGTCGAGGCAGAAAATTCCAATGGGATACCTGATGCAGAACATCTGCTTCCAGAGTTCCAGATCTCTGCTTCTGGCAATGGGAAGTATTTGACCGGTGCTGGTAATGGTTCTGTAACTGCAGTCGTAAATGGATTTGGTGAAGGGGAGAAAGGTTCCTTGTTAGCCGCTAATTTATCTACTAACACGAGCAACAATCATTCTGAGAGTGTCTTATCTAAAGCAGTGAAGTTGGAAAGGAAGAGTAATGGTAGAGCCCCTTTAGTTTCAAGTGATGATGAATTGGGATCAATTGAAGGAGATATGTGTGCTTCTGCAACTGGGGTTGTAAGGGTGCAGTCAAGAAAGAAAGCAGAGATGTTTTTAGTACGAACGGATGGATTCTCTTGTACCCGAGAAAAAGTGACAGAATCCTCCCTGGCCTTTACTCATCCAAGTACTCAGCAACAAATGCTTATGTGGAAATCTACACCAAAGACAGTATTAGTGTTAAAGAAGCTTGGGCATGAGCTTATGGAACAAGCTAAAGAGGTTGTCTCCTTCTTGTATTACCAAGAGAAAATGAATGTTCTGGTGGAACCTGACGTGCATGATGTATTTGCTCGAATCCCAGGTTTTGGATTTGTTCAGACCTTCTACACCCAAGATACCGGTGATCTGCATGAGAGGGTTGATTTTGTGGCATGTCTGGGAGGGGATGGGGTAATACTTCATGCTTCAAATCTATTCAAAGGTGCTGTTCCCCCCATCGTATCATTTAATCTTGGATCTCTTGGATTTTTGACTTCCCATACTTTTGATGACTACAGGCAGGACTTAAGACAGGTTATCCATGGAAATAACACAAGTGATGGTGTTTATATAACTCTCAGAATGCGTCTCAGGTGTGAAATTTTTCGAAATGGTAAAGCGATGCCTGGAAAGGTATTTGATGTTTTGAATGAAATAGTTGTTGATCGAGGTTCGAATCCGTACCTTTCTAAGATTGAATGTTATGAACGTGACCAGCTTATAACCAAGGTCCAAGGTGATGGTGTCATTGTAGCCACACCTACAGGAAGTACTGCTTATTCTACAGCTGCAGGAGGTTCCATGGTGCATCCAAATGTTCCTTGCATGTTGTTTACGCCAATCTGCCCACACTCCCTCTCATTTAGACCAGTTATACTTCCAGATTCTGCAAGCCTTGAATTAAAGATTCCAGAGGATGCTCGAAGCAATGCCTGGGTTTCTTTTGATGGGAAGAGAAGGCAGCAGCTCTCAAGGGGAGACTCTGTTCGAATAGCTATGAGCCAACACCCACTTCCAACAGTCAACAAGCGTGACCAAACAGGGGACTGGTTTCGAAGCTTGATTCGTTGCCTAAATTGGAATGAGAGGCTAGATCAAAAGGCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

1004

Amino Acids

111.76

Weight (kDa)

6.08

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-NADK PF22741 247 - 392 1.6e-63 DSP-PTPase phosphatase fused to NAD+ Kinase
Tc-R-P PF22785 281 - 375 2.8e-11 Polymorphic toxin system, DSP-PTPase phosphatase
NAD_kinase PF01513 707 - 817 4.7e-15 ATP-NAD kinase N-terminal domain
NAD_kinase_C PF20143 851 - 977 6.5e-35 ATP-NAD kinase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2627
Acc36I ACCTGC 2 cut(s) 1012, 1337
AccB7I CCANNNNNTGG 1 cut(s) 2231
AccBSI CCGCTC 1 cut(s) 421
AccI GTMKAC 1 cut(s) 1299
AccII CGCG 2 cut(s) 259, 726
AciI CCGC 9 cut(s) 52, 60, 105, 160, 182, 259, 419, 1609, 1782
AclWI GGATC 3 cut(s) 239, 1909, 2389
AcoI YGGCCR 2 cut(s) 30, 838
AcsI RAATTY 5 cut(s) 312, 984, 1031, 1630, 2505
AcuI CTGAAG 3 cut(s) 617, 668, 713
AfaI GTAC 5 cut(s) 1983, 2005, 2053, 2588, 2677
AfiI CCNNNNNNNGG 6 cut(s) 278, 374, 409, 1125, 2231, 2947
AflII CTTAAG 1 cut(s) 2431
AgeI ACCGGT 2 cut(s) 1709, 2264
AjiI CACGTC 1 cut(s) 2200
AjnI CCWGG 9 cut(s) 24, 277, 646, 840, 1469, 2031, 2224, 2530, 2837
AjuI GAANNNNNNNTTGG 2 cut(s) 2901, 2933
AloI GAACNNNNNNTCC 2 cut(s) 2874, 2906
Alw21I GWGCWC 1 cut(s) 521
Alw26I GTCTC 6 cut(s) 347, 1097, 1165, 2155, 2498, 2877
AlwI GGATC 3 cut(s) 239, 1909, 2389
AlwNI CAGNNNCTG 2 cut(s) 691, 2792
Ama87I CYCGRG 1 cut(s) 2007
AoxI GGCC 5 cut(s) 30, 268, 838, 2034, 3006
ApeKI GCWGC 6 cut(s) 938, 1056, 1129, 2693, 2866, 2869
ApoI RAATTY 5 cut(s) 312, 984, 1031, 1630, 2505
AsiGI ACCGGT 2 cut(s) 1709, 2264
Asp700I GAANNNNTTC 1 cut(s) 2508
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 6 cut(s) 145, 269, 804, 1326, 2635, 3007
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 7 cut(s) 57, 382, 1405, 1595, 1766, 2279, 2654
AsuII TTCGAA 5 cut(s) 319, 2512, 2579, 2893, 2959
AvaI CYCGRG 1 cut(s) 2007
AvaII GGWCC 4 cut(s) 145, 804, 1326, 2635
BaeGI GKGCMC 1 cut(s) 83
BalI TGGCCA 2 cut(s) 32, 840
BanII GRGCYC 2 cut(s) 521, 1873
BauI CACGAG 1 cut(s) 1801
Bbv12I GWGCWC 1 cut(s) 521
BbvCI CCTCAGC 1 cut(s) 546
BbvI GCAGC 6 cut(s) 950, 1068, 1141, 2680, 2878, 2881
BceAI ACGGC 3 cut(s) 23, 926, 1128
BcgI CGANNNNNNTGC 2 cut(s) 1121, 1155
BciT130I CCWGG 9 cut(s) 26, 279, 648, 842, 1471, 2033, 2226, 2532, 2839
BciVI GTATCC 1 cut(s) 1635
BclI TGATCA 1 cut(s) 978
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 6 cut(s) 347, 1097, 1165, 2155, 2498, 2877
BfaI CTAG 1 cut(s) 2996
BfmI CTRYAG 7 cut(s) 1296, 1402, 1734, 2419, 2667, 2688, 2694
BfrI CTTAAG 1 cut(s) 2431
BfuAI ACCTGC 2 cut(s) 1012, 1337
BfuI GTATCC 1 cut(s) 1635
BglII AGATCT 1 cut(s) 1677
BmcAI AGTACT 2 cut(s) 2053, 2677
Bme18I GGWCC 4 cut(s) 145, 804, 1326, 2635
BmeT110I CYCGRG 1 cut(s) 2007
BmgBI CACGTC 1 cut(s) 2200
BmgT120I GGNCC 6 cut(s) 145, 269, 804, 1326, 2635, 3007
BmiI GGNNCC 5 cut(s) 1327, 1434, 1771, 2193, 2704
BmrI ACTGGG 1 cut(s) 1944
BmsI GCATC 7 cut(s) 633, 940, 968, 1639, 2517, 2722, 2812
BmuI ACTGGG 1 cut(s) 1944
BplI GAGNNNNNCTC 2 cut(s) 2135, 2167
BpmI CTGGAG 4 cut(s) 71, 881, 893, 1117
Bpu10I CCTNAGC 2 cut(s) 546, 1428
Bpu14I TTCGAA 5 cut(s) 319, 2512, 2579, 2893, 2959
BpuEI CTTGAG 6 cut(s) 137, 149, 650, 1438, 1499, 2860
BpuMI CCSGG 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 1097
BsaWI WCCGGW 4 cut(s) 367, 713, 1709, 2264
BsaXI ACNNNNNCTCC 4 cut(s) 344, 374, 2874, 2904
Bsc4I CCNNNNNNNGG 6 cut(s) 278, 374, 409, 1125, 2231, 2947
Bse118I RCCGGY 3 cut(s) 147, 1709, 2264
Bse1I ACTGG 8 cut(s) 88, 641, 876, 989, 1254, 1939, 2774, 2957
Bse3DI GCAATG 2 cut(s) 1699, 2839
BseBI CCWGG 9 cut(s) 26, 279, 648, 842, 1471, 2033, 2226, 2532, 2839
BseLI CCNNNNNNNGG 6 cut(s) 278, 374, 409, 1125, 2231, 2947
BseMI GCAATG 2 cut(s) 1699, 2839
BseMII CTCAG 9 cut(s) 108, 220, 239, 537, 1442, 1809, 2069, 2497, 2509
BseNI ACTGG 8 cut(s) 88, 641, 876, 989, 1254, 1939, 2774, 2957
BseRI GAGGAG 3 cut(s) 543, 569, 743
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 6 cut(s) 950, 1068, 1141, 2680, 2878, 2881
BsgI GTGCAG 3 cut(s) 403, 990, 1970
Bsh1236I CGCG 2 cut(s) 259, 726
BshFI GGCC 5 cut(s) 32, 270, 840, 2036, 3008
BshTI ACCGGT 2 cut(s) 1709, 2264
BsiHKAI GWGCWC 1 cut(s) 521
BsiHKCI CYCGRG 1 cut(s) 2007
BsiSI CCGG 6 cut(s) 148, 368, 375, 714, 1710, 2265
BslFI GGGAC 3 cut(s) 1015, 1339, 2963
BslI CCNNNNNNNGG 6 cut(s) 278, 374, 409, 1125, 2231, 2947
BsmAI GTCTC 6 cut(s) 347, 1097, 1165, 2155, 2498, 2877
BsmBI CGTCTC 1 cut(s) 2498
BsmFI GGGAC 3 cut(s) 1015, 1339, 2963
BsmI GAATGC 2 cut(s) 1429, 2493
BsnI GGCC 5 cut(s) 32, 270, 840, 2036, 3008
Bso31I GGTCTC 1 cut(s) 1097
BsoBI CYCGRG 1 cut(s) 2007
Bsp119I TTCGAA 5 cut(s) 319, 2512, 2579, 2893, 2959
Bsp1286I GDGCHC 3 cut(s) 83, 521, 1873
Bsp19I CCATGG 2 cut(s) 2446, 2706
BspACI CCGC 9 cut(s) 52, 60, 105, 160, 182, 259, 419, 1609, 1782
BspANI GGCC 5 cut(s) 32, 270, 840, 2036, 3008
BspCNI CTCAG 9 cut(s) 107, 219, 238, 538, 1441, 1810, 2068, 2496, 2508
BspFNI CGCG 2 cut(s) 259, 726
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 5 cut(s) 1327, 1434, 1771, 2193, 2704
BspMAI CTGCAG 3 cut(s) 1406, 1738, 2698
BspMI ACCTGC 2 cut(s) 1012, 1337
BspPI GGATC 3 cut(s) 239, 1909, 2389
BspT104I TTCGAA 5 cut(s) 319, 2512, 2579, 2893, 2959
BspTI CTTAAG 1 cut(s) 2431
BspTNI GGTCTC 1 cut(s) 1097
BsrBI CCGCTC 1 cut(s) 421
BsrDI GCAATG 2 cut(s) 1699, 2839
BsrFI RCCGGY 3 cut(s) 147, 1709, 2264
BsrI ACTGG 8 cut(s) 88, 641, 876, 989, 1254, 1939, 2774, 2957
BssAI RCCGGY 3 cut(s) 147, 1709, 2264
BssSI CACGAG 1 cut(s) 1801
BssT1I CCWWGG 5 cut(s) 1351, 2446, 2631, 2638, 2706
Bst2BI CACGAG 1 cut(s) 1801
Bst2UI CCWGG 9 cut(s) 26, 279, 648, 842, 1471, 2033, 2226, 2532, 2839
Bst4CI ACNGT 3 cut(s) 468, 2095, 2926
Bst6I CTCTTC 4 cut(s) 593, 790, 1850, 2852
BstAFI CTTAAG 1 cut(s) 2431
BstAPI GCANNNNNTGC 1 cut(s) 2065
BstBI TTCGAA 5 cut(s) 319, 2512, 2579, 2893, 2959
BstC8I GCNNGC 2 cut(s) 617, 2797
BstDSI CCRYGG 2 cut(s) 2446, 2706
BstEII GGTNACC 1 cut(s) 370
BstFNI CGCG 2 cut(s) 259, 726
BstHHI GCGC 1 cut(s) 628
BstMAI GTCTC 6 cut(s) 347, 1097, 1165, 2155, 2498, 2877
BstMWI GCNNNNNNNGC 5 cut(s) 21, 157, 193, 625, 2065
BstNI CCWGG 9 cut(s) 26, 279, 648, 842, 1471, 2033, 2226, 2532, 2839
BstNSI RCATGY 4 cut(s) 12, 565, 2301, 2734
BstPI GGTNACC 1 cut(s) 370
BstSFI CTRYAG 7 cut(s) 1296, 1402, 1734, 2419, 2667, 2688, 2694
BstSLI GKGCMC 1 cut(s) 83
BstUI CGCG 2 cut(s) 259, 726
BstV1I GCAGC 6 cut(s) 950, 1068, 1141, 2680, 2878, 2881
BstX2I RGATCY 2 cut(s) 1677, 2381
BstXI CCANNNNNNTGG 1 cut(s) 2645
BstYI RGATCY 2 cut(s) 1677, 2381
BsuI GTATCC 1 cut(s) 1635
BsuRI GGCC 5 cut(s) 32, 270, 840, 2036, 3008
BtgI CCRYGG 2 cut(s) 2446, 2706
BtgZI GCGATG 2 cut(s) 19, 2540
BtrI CACGTC 1 cut(s) 2200
BtsI GCAGTG 2 cut(s) 391, 1845
BtsIMutI CAGTG 6 cut(s) 184, 391, 464, 1247, 1611, 1845
BveI ACCTGC 2 cut(s) 1012, 1337
Cac8I GCNNGC 2 cut(s) 617, 2797
CaiI CAGNNNCTG 2 cut(s) 691, 2792
CciI TCATGA 1 cut(s) 241
CfoI GCGC 1 cut(s) 628
Cfr10I RCCGGY 3 cut(s) 147, 1709, 2264
Cfr13I GGNCC 6 cut(s) 145, 269, 804, 1326, 2635, 3007
CseI GACGC 1 cut(s) 2480
Csp6I GTAC 5 cut(s) 1982, 2004, 2052, 2587, 2676
CspAI ACCGGT 2 cut(s) 1709, 2264
CviQI GTAC 5 cut(s) 1982, 2004, 2052, 2587, 2676
DraI TTTAAA 1 cut(s) 886
EaeI YGGCCR 2 cut(s) 30, 838
Eam1104I CTCTTC 4 cut(s) 593, 790, 1850, 2852
EarI CTCTTC 4 cut(s) 593, 790, 1850, 2852
Ecl136II GAGCTC 1 cut(s) 519
Eco130I CCWWGG 5 cut(s) 1351, 2446, 2631, 2638, 2706
Eco24I GRGCYC 2 cut(s) 521, 1873
Eco31I GGTCTC 1 cut(s) 1097
Eco47I GGWCC 4 cut(s) 145, 804, 1326, 2635
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 3 cut(s) 617, 668, 713
Eco88I CYCGRG 1 cut(s) 2007
Eco91I GGTNACC 1 cut(s) 370
EcoICRI GAGCTC 1 cut(s) 519
EcoO109I RGGNCCY 2 cut(s) 1326, 3007
EcoO65I GGTNACC 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 984
EcoRII CCWGG 9 cut(s) 24, 277, 646, 840, 1469, 2031, 2224, 2530, 2837
EcoT14I CCWWGG 5 cut(s) 1351, 2446, 2631, 2638, 2706
EcoT38I GRGCYC 2 cut(s) 521, 1873
ErhI CCWWGG 5 cut(s) 1351, 2446, 2631, 2638, 2706
Esp3I CGTCTC 1 cut(s) 2498
FalI AAGNNNNNCTT 6 cut(s) 407, 439, 1372, 1404, 2665, 2697
FaqI GGGAC 3 cut(s) 1015, 1339, 2963
FauI CCCGC 2 cut(s) 112, 1602
FbaI TGATCA 1 cut(s) 978
FblI GTMKAC 1 cut(s) 1299
FriOI GRGCYC 2 cut(s) 521, 1873
FspBI CTAG 1 cut(s) 2996
GlaI GCGC 1 cut(s) 627
GsuI CTGGAG 4 cut(s) 71, 881, 893, 1117
HaeIII GGCC 5 cut(s) 32, 270, 840, 2036, 3008
HapII CCGG 6 cut(s) 148, 368, 375, 714, 1710, 2265
HgaI GACGC 1 cut(s) 2480
HhaI GCGC 1 cut(s) 628
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HincII GTYRAC 2 cut(s) 1414, 2929
HindII GTYRAC 2 cut(s) 1414, 2929
HindIII AAGCTT 2 cut(s) 2111, 2962
HpaII CCGG 6 cut(s) 148, 368, 375, 714, 1710, 2265
HphI GGTGA 7 cut(s) 57, 382, 1405, 1595, 1766, 2279, 2654
Hpy166II GTNNAC 7 cut(s) 775, 1300, 1385, 1396, 1414, 2739, 2929
Hpy8I GTNNAC 7 cut(s) 775, 1300, 1385, 1396, 1414, 2739, 2929
Hpy99I CGWCG 3 cut(s) 44, 176, 253
HpyCH4III ACNGT 3 cut(s) 468, 2095, 2926
HpyCH4IV ACGT 3 cut(s) 621, 2199, 2614
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 157, 193, 625, 2065
HpySE526I ACGT 3 cut(s) 621, 2199, 2614
HspAI GCGC 1 cut(s) 626
Ksp22I TGATCA 1 cut(s) 978
LmnI GCTCC 3 cut(s) 184, 524, 1018
Lsp1109I GCAGC 6 cut(s) 950, 1068, 1141, 2680, 2878, 2881
LweI GCATC 7 cut(s) 633, 940, 968, 1639, 2517, 2722, 2812
MaeI CTAG 1 cut(s) 2996
MaeII ACGT 3 cut(s) 621, 2199, 2614
MaeIII GTNAC 9 cut(s) 370, 462, 844, 867, 1245, 1729, 2017, 2615, 2936
MbiI CCGCTC 1 cut(s) 421
MfeI CAATTG 1 cut(s) 1905
MflI RGATCY 2 cut(s) 1677, 2381
MhlI GDGCHC 3 cut(s) 83, 521, 1873
MlsI TGGCCA 2 cut(s) 32, 840
MluNI TGGCCA 2 cut(s) 32, 840
MlyI GAGTC 5 cut(s) 215, 760, 1132, 1253, 2879
MmeI TCCRAC 3 cut(s) 1174, 1827, 2945
Mox20I TGGCCA 2 cut(s) 32, 840
MroXI GAANNNNTTC 1 cut(s) 2508
MscI TGGCCA 2 cut(s) 32, 840
MseI TTAA 8 cut(s) 885, 1182, 1512, 1523, 2105, 2373, 2432, 2807
MslI CAYNNNNRTG 1 cut(s) 2718
Msp20I TGGCCA 2 cut(s) 32, 840
MspA1I CMGCKG 2 cut(s) 54, 2693
MspCI CTTAAG 1 cut(s) 2431
MspI CCGG 6 cut(s) 148, 368, 375, 714, 1710, 2265
MunI CAATTG 1 cut(s) 1905
Mva1269I GAATGC 2 cut(s) 1429, 2493
MvaI CCWGG 9 cut(s) 26, 279, 648, 842, 1471, 2033, 2226, 2532, 2839
MvnI CGCG 2 cut(s) 259, 726
MwoI GCNNNNNNNGC 5 cut(s) 21, 157, 193, 625, 2065
NciI CCSGG 1 cut(s) 376
NcoI CCATGG 2 cut(s) 2446, 2706
NlaIV GGNNCC 5 cut(s) 1327, 1434, 1771, 2193, 2704
NmuCI GTSAC 6 cut(s) 370, 462, 1245, 2017, 2615, 2936
NspI RCATGY 4 cut(s) 12, 565, 2301, 2734
NspV TTCGAA 5 cut(s) 319, 2512, 2579, 2893, 2959
PagI TCATGA 1 cut(s) 241
PctI GAATGC 2 cut(s) 1429, 2493
PdmI GAANNNNTTC 1 cut(s) 2508
PflMI CCANNNNNTGG 1 cut(s) 2231
PinAI ACCGGT 2 cut(s) 1709, 2264
PleI GAGTC 5 cut(s) 215, 760, 1132, 1252, 2879
PpsI GAGTC 5 cut(s) 215, 760, 1132, 1252, 2879
PpuMI RGGWCCY 1 cut(s) 1326
PsiI TTATAA 1 cut(s) 2627
Psp124BI GAGCTC 1 cut(s) 521
Psp5II RGGWCCY 1 cut(s) 1326
Psp6I CCWGG 9 cut(s) 24, 277, 646, 840, 1469, 2031, 2224, 2530, 2837
PspEI GGTNACC 1 cut(s) 370
PspGI CCWGG 9 cut(s) 24, 277, 646, 840, 1469, 2031, 2224, 2530, 2837
PspN4I GGNNCC 5 cut(s) 1327, 1434, 1771, 2193, 2704
PspPI GGNCC 6 cut(s) 145, 269, 804, 1326, 2635, 3007
PspPPI RGGWCCY 1 cut(s) 1326
PstI CTGCAG 3 cut(s) 1406, 1738, 2698
PstNI CAGNNNCTG 2 cut(s) 691, 2792
PsuI RGATCY 2 cut(s) 1677, 2381
PvuII CAGCTG 1 cut(s) 2693
RsaI GTAC 5 cut(s) 1983, 2005, 2053, 2588, 2677
RsaNI GTAC 5 cut(s) 1982, 2004, 2052, 2587, 2676
RseI CAYNNNNRTG 1 cut(s) 2718
SacI GAGCTC 1 cut(s) 521
SaqAI TTAA 8 cut(s) 885, 1182, 1512, 1523, 2105, 2373, 2432, 2807
Sau96I GGNCC 6 cut(s) 145, 269, 804, 1326, 2635, 3007
ScaI AGTACT 2 cut(s) 2053, 2677
SchI GAGTC 5 cut(s) 215, 760, 1132, 1253, 2879
SduI GDGCHC 3 cut(s) 83, 521, 1873
SfaNI GCATC 7 cut(s) 633, 940, 968, 1639, 2517, 2722, 2812
SfcI CTRYAG 7 cut(s) 1296, 1402, 1734, 2419, 2667, 2688, 2694
SfuI TTCGAA 5 cut(s) 319, 2512, 2579, 2893, 2959
SinI GGWCC 4 cut(s) 145, 804, 1326, 2635
SmiMI CAYNNNNRTG 1 cut(s) 2718
SmlI CTYRAG 7 cut(s) 152, 164, 629, 1453, 1478, 2431, 2875
SmoI CTYRAG 7 cut(s) 152, 164, 629, 1453, 1478, 2431, 2875
SsiI CCGC 9 cut(s) 52, 60, 105, 160, 182, 259, 419, 1609, 1782
SspI AATATT 3 cut(s) 928, 1447, 1492
SspMI CTAG 1 cut(s) 2996
SstI GAGCTC 1 cut(s) 521
StyI CCWWGG 5 cut(s) 1351, 2446, 2631, 2638, 2706
TaaI ACNGT 3 cut(s) 468, 2095, 2926
TaiI ACGT 3 cut(s) 624, 2202, 2617
TatI WGTACW 2 cut(s) 2051, 2675
TauI GCSGC 5 cut(s) 54, 62, 163, 421, 1784
Tru1I TTAA 8 cut(s) 885, 1182, 1512, 1523, 2105, 2373, 2432, 2807
Tru9I TTAA 8 cut(s) 885, 1182, 1512, 1523, 2105, 2373, 2432, 2807
TscAI CASTG 6 cut(s) 184, 391, 471, 1254, 1611, 1845
TseFI GTSAC 6 cut(s) 370, 462, 1245, 2017, 2615, 2936
TseI GCWGC 6 cut(s) 938, 1056, 1129, 2693, 2866, 2869
Tsp45I GTSAC 6 cut(s) 370, 462, 1245, 2017, 2615, 2936
TspGWI ACGGA 4 cut(s) 237, 729, 2003, 2574
TspRI CASTG 6 cut(s) 184, 391, 471, 1254, 1611, 1845
Van91I CCANNNNNTGG 1 cut(s) 2231
Vha464I CTTAAG 1 cut(s) 2431
VpaK11BI GGWCC 4 cut(s) 145, 804, 1326, 2635
XapI RAATTY 5 cut(s) 312, 984, 1031, 1630, 2505
XceI RCATGY 4 cut(s) 12, 565, 2301, 2734
XmiI GTMKAC 1 cut(s) 1299
XmnI GAANNNNTTC 1 cut(s) 2508
XspI CTAG 1 cut(s) 2996
ZrmI AGTACT 2 cut(s) 2053, 2677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.