FvH4_2g27820

NAD kinase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010084
Physical Location & Seq
Reverse (-)
21974643 .. 21979477
4835 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27820.t1

Sequence Viewer

Length: 2970 bp
ATGGTGGCATGTTGCCCGATGTGCCAGGTGGCCATGGCCGTCGATATGAGCCGCTGTGCTGCTCCACCTCCCAATCTCTGCTCCCTCAAGCCTCCTCAGTTATCGGGTTGCCCCGCCAAGCTATTCGGATTCGGGTTCGGATTCGACATTCACCTAAAGCACCGCCTCAAGCGGCGCCTCAAGTTCGTCGTCGCTGCGGAGCTCTCCCAGCCTTTCTCCTCTCTCAGCTTCGGCTTCGACTCTCAGGCGTCGCAGCCTCATGATCCGTCGCCGAGCCTCGGCCCTGTGCCTGGAGACATCGCCGAAATCGAGGCCTACTGTAGAATCTTCAGAGCCGCGGAACGGCTACACACTGCATTGATGGAGGCGTTGTGCAATCCGGTGACCGGAGTATGCAGCGTTTATTACGACTTCCCGTCGGAGGAGAAGCCGCTGCTGGAGGATAAGATTGTGGCGGTGATTGGTTGCATGGCGTCGCTGTTGAACAAGGCGAGGGAGGATGTGCTCTCTGGAAGATCGTCGTTTCGAGTTGTGGATGTTGGTGTGGTGGTGGAGGATAAGCTTCCTCCACTGGCTGTGTTCAGGAGTGAGATGAAGAGGTGTTGCGAGAGCTTGCACGTTGCGCTGGAGGATTGGCTGATGCCTGGGGATGATCAGAGTGTGGATGTGTGGAGGAAGCTTCAGAGGTTGAAGAATGTGTGTTATGATTGTGGTTTTGCGCGGAATGAGGATGATCCGTGCAATAGTCTGTTTGCGAATTGGGGTCCTGTTTACTTCTCCTCTGATGATTCCGAGGTGGCTTTTTGGAGGGGTGGCCAGGTAACAGAAGAGGGCTTGAAGTTGTTACTGGACAAGGGATTTAAAACTATTGTTGATATTAGAGCAGAGAATGTGAAAGACAACATTTTTCAGGGAGCCTTAGATGATGCTATTGCATCAGGAAAGGTGGAGTTGATCAGGATTCCGGTTGAAGTTGGGACTGCTCCTTCAATGGAGCAGGTTGAGAAATTTGCGAGTTTGGTTTCAGATAGCACCAAAAGGCCGATTTATCTTCATAGTAAGGAAGGTCTGTTGAGAGCATCTGCCATGGTATCCAGATGGAGGCAGCACTTGACTCGGCGTGCTGTCTCCAAGCAGTCGGTTTCTCTAAATGGAGAAGTTGGAAAACCCTCAACTACGGAGAAAAATGCCCTCCTTGAAAAGACAATGCATGGTTCAAATGGTGTACTACAAAAAAATGATTCTGTAGAGAGCGATGAAGCAAATCTGAATGGGACCTGCAATGGCCTTATATCTATCCAAGGTATGAAATCAGTTGAATCAGATGAAAGTGAGGAAAAACCTTTGGTGAACTTTAGCAGGGAAGTTGACCCCCTAAATGCTCAGGTTCCTCCTTGTAATGTCTTCTCAAGAAAAGAAATGTCCAGGTTCCTTGCAAGAAAAAATATTGCGCCTCTCACTTATTTTAATTATCAGTTAAATAGGTTGGAGGTATTACCAATTTCCAGATATATGAATACTAAGATAATGTGGAGAGGTGAGATTGTTGGTATAGATCCAGTGCGGGAGGTTGTGGAGGCAGAAAATTCCAATGGGATACCTGATGCAAAACATCTGCTTCCAGAGTCCCAGATCTCTGCTTCTGGTAATGGGGTGTATTTGACCAGTGCTGGTAATGGTTCTGCAACTGCAGTCGTAAATGAATTTGGTGAAGGGGAGAACTGTTCCTTGTTAACCACTAATTCATCTACTAATGTGAGCAACACTCATTCTGAGAGTGTCTTATCTAAAGTAGTGAAGGAGGTTAGGAAGAGTAATGGTCAAGCCCCTTTAGTTTCAAGTGATGATGAATTGGGATCAATTGAAGGGGATATGTGTGCTTCTGCAACTGGTGTAGTAAGGGTGCAGTCAAGAAAGAAAGCAGAGATGTTTTTAGTACGAACAGATGGATTCTCTTGTACCCGAGAAAAAGTGACAGAATCCTCCCTGGCCTTCACTCATCCAAGTACTCAGCAACAAATGCTTATGTGGAAATCTACACCAAAGACAGTATTAGTGTTAAAAAAGCTTGGGCACGAGCTCATGGAACAAGCTAAAGAGGTTGTCTCCTTCTTGTATTACAAAGAGAAAATGAATGTTCTGGTGGAACCCGATGTGCATGATGTATTTGCTAGGATCCCAGGTTTTGGATTTGTTCAGACATTCTACACCCAAGATACTGGTGATCTTCATGAGAGGGTTGATTTTGTGGCTTGTCTGGGAGGGGATGGGGTAATACTCCATGCTTCAAATCTATTCAAAGGTGCTGTCCCCCCCATCGTGTCATTTAATCTCGGATCTCTTGGATTTTTGACTTCCCATACTTTTGATGACTACAGGCAGGACTTAAGGCAGGTCATCCATGGAAATAACACACGTGATGGTGTTTATATAACTCTCAGAATGCGTCTCAGGTGTGAAATATTTCGAAATGGTAATGCGATGCCTGGAAAGGTGTTTGATGTTTTGAATGAAATAGTTGTTGATCGAGGTTCGAATCCGTACCTTTCGAAGATCGAATGTTATGAACGCGACCAGCTTATAACCAAGGTCCAAGGTGATGGTGTCATTGTAGCCACACCTACAGGAAGTACTGCTTATTCTACAGCTGCAGGAGGTTCCATGGTGCATCCAAATGTTCCTTGCATGTTGTTTACGCCGATCTGCCCACACTCCCTCTCATTTAGACCAGTCATACTTCCAGATTCTGCAAGCCTTGAATTAAAGATTCCAAAGGATGCTCGAAGCAATGCCTGGGTTTCTTTTGACGGGAAGAGGAGGCAGCAACTCTCAAGGGGAGACTCTGTTCGAATAGCTATGAGCGAACACCCACTTCCAACTGTCAACAAGCGTGACCAAACAGGTGACTGGTTTCGAAGCTTGATTCGTTGCCTAAATTGGAATGAGAGGCTAGATCAAAAGGCCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

990

Amino Acids

109.28

Weight (kDa)

6.26

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-NADK PF22741 246 - 384 3.1e-56 DSP-PTPase phosphatase fused to NAD+ Kinase
Tc-R-P PF22785 273 - 367 7.9e-09 Polymorphic toxin system, DSP-PTPase phosphatase
NAD_kinase PF01513 692 - 802 5.5e-15 ATP-NAD kinase N-terminal domain
NAD_kinase_C PF20143 836 - 962 5.4e-35 ATP-NAD kinase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2582
Acc36I ACCTGC 3 cut(s) 988, 1286, 2383
AccB1I GGYRCC 1 cut(s) 174
AccB7I CCANNNNNTGG 1 cut(s) 2186
AccII CGCG 3 cut(s) 338, 721, 2571
AclWI GGATC 7 cut(s) 257, 728, 1550, 1864, 2170, 2183, 2344
AcoI YGGCCR 3 cut(s) 30, 36, 814
AcsI RAATTY 3 cut(s) 1007, 1585, 1703
AcuI CTGAAG 2 cut(s) 313, 665
AcvI CACGTG 1 cut(s) 2417
AcyI GRCGYC 3 cut(s) 175, 248, 473
AfaI GTAC 6 cut(s) 1227, 1938, 1960, 2008, 2543, 2632
AfiI CCNNNNNNNGG 7 cut(s) 278, 290, 342, 386, 421, 1101, 2186
AflII CTTAAG 1 cut(s) 2386
AflIII ACRYGT 1 cut(s) 2414
AhdI GACNNNNNGTC 1 cut(s) 415
AjnI CCWGG 9 cut(s) 24, 289, 643, 816, 1424, 1986, 2179, 2485, 2792
AloI GAACNNNNNNTCC 2 cut(s) 2829, 2861
Alw21I GWGCWC 3 cut(s) 204, 507, 2082
Alw26I GTCTC 5 cut(s) 288, 1132, 2110, 2453, 2832
AlwI GGATC 7 cut(s) 257, 728, 1550, 1864, 2170, 2183, 2344
AlwNI CAGNNNCTG 1 cut(s) 2747
Ama87I CYCGRG 1 cut(s) 1962
AoxI GGCC 9 cut(s) 30, 36, 280, 312, 814, 1040, 1285, 1989, 2961
ApeKI GCWGC 8 cut(s) 59, 194, 253, 396, 433, 1105, 2648, 2821
ApoI RAATTY 3 cut(s) 1007, 1585, 1703
ArsI GACNNNNNNTTYG 2 cut(s) 2292, 2324
Asp700I GAANNNNTTC 1 cut(s) 2463
AspLEI GCGC 4 cut(s) 177, 625, 721, 1453
AspS9I GGNCC 5 cut(s) 281, 764, 1275, 2590, 2962
AsuHPI GGTGA 9 cut(s) 143, 394, 469, 1360, 1550, 1721, 2234, 2609, 2915
AsuII TTCGAA 5 cut(s) 2467, 2534, 2549, 2848, 2914
AvaI CYCGRG 1 cut(s) 1962
AvaII GGWCC 3 cut(s) 764, 1275, 2590
BaeGI GKGCMC 1 cut(s) 2076
BalI TGGCCA 2 cut(s) 32, 816
BamHI GGATCC 1 cut(s) 2175
BanI GGYRCC 1 cut(s) 174
BanII GRGCYC 2 cut(s) 204, 2082
BauI CACGAG 1 cut(s) 2075
BbrPI CACGTG 1 cut(s) 2417
BbsI GAAGAC 1 cut(s) 1396
Bbv12I GWGCWC 3 cut(s) 204, 507, 2082
BbvI GCAGC 8 cut(s) 46, 181, 265, 408, 420, 1117, 2635, 2833
BccI CCATC 7 cut(s) 355, 1092, 1940, 2261, 2324, 2414, 2594
BceAI ACGGC 2 cut(s) 23, 359
BcgI CGANNNNNNTGC 2 cut(s) 1097, 1131
BciT130I CCWGG 9 cut(s) 26, 291, 645, 818, 1426, 1988, 2181, 2487, 2794
BciVI GTATCC 2 cut(s) 1102, 1590
BclI TGATCA 2 cut(s) 652, 954
BcoDI GTCTC 5 cut(s) 288, 1132, 2110, 2453, 2832
BfaI CTAG 2 cut(s) 2172, 2951
BfmI CTRYAG 7 cut(s) 319, 1245, 1689, 2374, 2622, 2643, 2649
BfoI RGCGCY 1 cut(s) 178
BfrI CTTAAG 1 cut(s) 2386
BfuAI ACCTGC 3 cut(s) 988, 1286, 2383
BfuI GTATCC 2 cut(s) 1102, 1590
BglII AGATCT 1 cut(s) 1632
BmcAI AGTACT 2 cut(s) 2008, 2632
Bme1390I CCNGG 9 cut(s) 26, 291, 645, 818, 1426, 1988, 2181, 2487, 2794
Bme18I GGWCC 3 cut(s) 764, 1275, 2590
BmeRI GACNNNNNGTC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 1962
BmgT120I GGNCC 5 cut(s) 281, 764, 1275, 2590, 2962
BmiI GGNNCC 9 cut(s) 176, 765, 916, 1276, 1389, 1430, 2148, 2177, 2659
BmrFI CCNGG 9 cut(s) 26, 291, 645, 818, 1426, 1988, 2181, 2487, 2794
BmsI GCATC 8 cut(s) 630, 916, 944, 1088, 1594, 2472, 2677, 2767
BpiI GAAGAC 1 cut(s) 1396
BplI GAGNNNNNCTC 4 cut(s) 1750, 1782, 2090, 2122
BpmI CTGGAG 3 cut(s) 312, 458, 647
Bpu10I CCTNAGC 1 cut(s) 1383
Bpu14I TTCGAA 5 cut(s) 2467, 2534, 2549, 2848, 2914
BpuEI CTTGAG 5 cut(s) 71, 152, 164, 1393, 2815
BsaAI YACGTR 1 cut(s) 2417
BsaHI GRCGYC 3 cut(s) 175, 248, 473
BsaWI WCCGGW 3 cut(s) 379, 386, 964
BsaXI ACNNNNNCTCC 4 cut(s) 356, 386, 2829, 2859
Bsc4I CCNNNNNNNGG 7 cut(s) 278, 290, 342, 386, 421, 1101, 2186
Bse1I ACTGG 8 cut(s) 576, 852, 1559, 1665, 1894, 2224, 2729, 2912
Bse3DI GCAATG 2 cut(s) 1288, 2794
BseBI CCWGG 9 cut(s) 26, 291, 645, 818, 1426, 1988, 2181, 2487, 2794
BseLI CCNNNNNNNGG 7 cut(s) 278, 290, 342, 386, 421, 1101, 2186
BseMI GCAATG 2 cut(s) 1288, 2794
BseMII CTCAG 8 cut(s) 110, 238, 257, 1397, 1764, 2024, 2452, 2464
BseNI ACTGG 8 cut(s) 576, 852, 1559, 1665, 1894, 2224, 2729, 2912
BseRI GAGGAG 5 cut(s) 84, 208, 437, 769, 2830
BseSI GKGCMC 1 cut(s) 2076
BseXI GCAGC 8 cut(s) 46, 181, 265, 408, 420, 1117, 2635, 2833
BseYI CCCAGC 1 cut(s) 207
BsgI GTGCAG 1 cut(s) 1925
Bsh1236I CGCG 3 cut(s) 338, 721, 2571
BshFI GGCC 9 cut(s) 32, 38, 282, 314, 816, 1042, 1287, 1991, 2963
BshNI GGYRCC 1 cut(s) 174
BsiHKAI GWGCWC 3 cut(s) 204, 507, 2082
BsiHKCI CYCGRG 1 cut(s) 1962
BsiSI CCGG 3 cut(s) 380, 387, 965
BslFI GGGAC 4 cut(s) 991, 1288, 1612, 2294
BslI CCNNNNNNNGG 7 cut(s) 278, 290, 342, 386, 421, 1101, 2186
BsmAI GTCTC 5 cut(s) 288, 1132, 2110, 2453, 2832
BsmBI CGTCTC 1 cut(s) 2453
BsmFI GGGAC 4 cut(s) 991, 1288, 1612, 2294
BsmI GAATGC 1 cut(s) 2448
BsnI GGCC 9 cut(s) 32, 38, 282, 314, 816, 1042, 1287, 1991, 2963
BsoBI CYCGRG 1 cut(s) 1962
Bsp119I TTCGAA 5 cut(s) 2467, 2534, 2549, 2848, 2914
Bsp1286I GDGCHC 4 cut(s) 204, 507, 2076, 2082
Bsp19I CCATGG 4 cut(s) 33, 1086, 2401, 2661
BspANI GGCC 9 cut(s) 32, 38, 282, 314, 816, 1042, 1287, 1991, 2963
BspCNI CTCAG 8 cut(s) 109, 237, 256, 1396, 1765, 2023, 2451, 2463
BspFNI CGCG 3 cut(s) 338, 721, 2571
BspHI TCATGA 2 cut(s) 259, 2230
BspLI GGNNCC 9 cut(s) 176, 765, 916, 1276, 1389, 1430, 2148, 2177, 2659
BspMAI CTGCAG 2 cut(s) 1693, 2653
BspMI ACCTGC 3 cut(s) 988, 1286, 2383
BspPI GGATC 7 cut(s) 257, 728, 1550, 1864, 2170, 2183, 2344
BspT104I TTCGAA 5 cut(s) 2467, 2534, 2549, 2848, 2914
BspT107I GGYRCC 1 cut(s) 174
BspTI CTTAAG 1 cut(s) 2386
BsrDI GCAATG 2 cut(s) 1288, 2794
BsrI ACTGG 8 cut(s) 576, 852, 1559, 1665, 1894, 2224, 2729, 2912
BssNI GRCGYC 3 cut(s) 175, 248, 473
BssSI CACGAG 1 cut(s) 2075
BssT1I CCWWGG 7 cut(s) 33, 1086, 1300, 2401, 2586, 2593, 2661
Bst2BI CACGAG 1 cut(s) 2075
Bst2UI CCWGG 9 cut(s) 26, 291, 645, 818, 1426, 1988, 2181, 2487, 2794
Bst4CI ACNGT 4 cut(s) 320, 1724, 2050, 2881
Bst6I CTCTTC 4 cut(s) 590, 822, 1805, 2807
BstACI GRCGYC 3 cut(s) 175, 248, 473
BstAFI CTTAAG 1 cut(s) 2386
BstAPI GCANNNNNTGC 1 cut(s) 2020
BstBAI YACGTR 1 cut(s) 2417
BstBI TTCGAA 5 cut(s) 2467, 2534, 2549, 2848, 2914
BstC8I GCNNGC 3 cut(s) 614, 1122, 2752
BstDSI CCRYGG 5 cut(s) 33, 336, 1086, 2401, 2661
BstEII GGTNACC 1 cut(s) 382
BstFNI CGCG 3 cut(s) 338, 721, 2571
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 4 cut(s) 177, 625, 721, 1453
BstMAI GTCTC 5 cut(s) 288, 1132, 2110, 2453, 2832
BstMWI GCNNNNNNNGC 4 cut(s) 21, 208, 622, 2020
BstNI CCWGG 9 cut(s) 26, 291, 645, 818, 1426, 1988, 2181, 2487, 2794
BstNSI RCATGY 2 cut(s) 12, 2689
BstPI GGTNACC 1 cut(s) 382
BstSCI CCNGG 9 cut(s) 24, 289, 643, 816, 1424, 1986, 2179, 2485, 2792
BstSFI CTRYAG 7 cut(s) 319, 1245, 1689, 2374, 2622, 2643, 2649
BstSLI GKGCMC 1 cut(s) 2076
BstUI CGCG 3 cut(s) 338, 721, 2571
BstV1I GCAGC 8 cut(s) 46, 181, 265, 408, 420, 1117, 2635, 2833
BstV2I GAAGAC 1 cut(s) 1396
BstX2I RGATCY 4 cut(s) 1555, 1632, 2175, 2336
BstXI CCANNNNNNTGG 2 cut(s) 2219, 2600
BstYI RGATCY 4 cut(s) 1555, 1632, 2175, 2336
BsuI GTATCC 2 cut(s) 1102, 1590
BsuRI GGCC 9 cut(s) 32, 38, 282, 314, 816, 1042, 1287, 1991, 2963
BtgI CCRYGG 5 cut(s) 33, 336, 1086, 2401, 2661
BtgZI GCGATG 3 cut(s) 283, 1269, 2495
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 4 cut(s) 351, 569, 1566, 1672
BveI ACCTGC 3 cut(s) 988, 1286, 2383
Cac8I GCNNGC 3 cut(s) 614, 1122, 2752
CaiI CAGNNNCTG 1 cut(s) 2747
CciI TCATGA 2 cut(s) 259, 2230
CfoI GCGC 4 cut(s) 177, 625, 721, 1453
Cfr13I GGNCC 5 cut(s) 281, 764, 1275, 2590, 2962
Cfr42I CCGCGG 1 cut(s) 339
CseI GACGC 3 cut(s) 237, 462, 2435
Csp6I GTAC 6 cut(s) 1226, 1937, 1959, 2007, 2542, 2631
CviQI GTAC 6 cut(s) 1226, 1937, 1959, 2007, 2542, 2631
DinI GGCGCC 1 cut(s) 176
DraI TTTAAA 1 cut(s) 862
DriI GACNNNNNGTC 1 cut(s) 415
EaeI YGGCCR 3 cut(s) 30, 36, 814
Eam1104I CTCTTC 4 cut(s) 590, 822, 1805, 2807
Eam1105I GACNNNNNGTC 1 cut(s) 415
EarI CTCTTC 4 cut(s) 590, 822, 1805, 2807
Ecl136II GAGCTC 2 cut(s) 202, 2080
Eco130I CCWWGG 7 cut(s) 33, 1086, 1300, 2401, 2586, 2593, 2661
Eco147I AGGCCT 1 cut(s) 314
Eco24I GRGCYC 2 cut(s) 204, 2082
Eco47I GGWCC 3 cut(s) 764, 1275, 2590
Eco53kI GAGCTC 2 cut(s) 202, 2080
Eco57I CTGAAG 2 cut(s) 313, 665
Eco72I CACGTG 1 cut(s) 2417
Eco88I CYCGRG 1 cut(s) 1962
Eco91I GGTNACC 1 cut(s) 382
EcoICRI GAGCTC 2 cut(s) 202, 2080
EcoO109I RGGNCCY 3 cut(s) 764, 1275, 2962
EcoO65I GGTNACC 1 cut(s) 382
EcoRII CCWGG 9 cut(s) 24, 289, 643, 816, 1424, 1986, 2179, 2485, 2792
EcoT14I CCWWGG 7 cut(s) 33, 1086, 1300, 2401, 2586, 2593, 2661
EcoT22I ATGCAT 1 cut(s) 1212
EcoT38I GRGCYC 2 cut(s) 204, 2082
EgeI GGCGCC 1 cut(s) 176
EheI GGCGCC 1 cut(s) 176
ErhI CCWWGG 7 cut(s) 33, 1086, 1300, 2401, 2586, 2593, 2661
Esp3I CGTCTC 1 cut(s) 2453
FalI AAGNNNNNCTT 2 cut(s) 2620, 2652
FaqI GGGAC 4 cut(s) 991, 1288, 1612, 2294
FauI CCCGC 2 cut(s) 121, 1557
FbaI TGATCA 2 cut(s) 652, 954
FriOI GRGCYC 2 cut(s) 204, 2082
FspBI CTAG 2 cut(s) 2172, 2951
GlaI GCGC 4 cut(s) 176, 624, 720, 1452
GsaI CCCAGC 1 cut(s) 211
GsuI CTGGAG 3 cut(s) 312, 458, 647
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 9 cut(s) 32, 38, 282, 314, 816, 1042, 1287, 1991, 2963
HapII CCGG 3 cut(s) 380, 387, 965
HgaI GACGC 3 cut(s) 237, 462, 2435
HhaI GCGC 4 cut(s) 177, 625, 721, 1453
Hin1I GRCGYC 3 cut(s) 175, 248, 473
Hin6I GCGC 4 cut(s) 175, 623, 719, 1451
HinP1I GCGC 4 cut(s) 175, 623, 719, 1451
HincII GTYRAC 3 cut(s) 1369, 1734, 2884
HindII GTYRAC 3 cut(s) 1369, 1734, 2884
HindIII AAGCTT 4 cut(s) 560, 677, 2066, 2917
HpaI GTTAAC 1 cut(s) 1734
HpaII CCGG 3 cut(s) 380, 387, 965
HphI GGTGA 9 cut(s) 143, 394, 469, 1360, 1550, 1721, 2234, 2609, 2915
Hpy166II GTNNAC 7 cut(s) 772, 1226, 1351, 1369, 1734, 2694, 2884
Hpy8I GTNNAC 7 cut(s) 772, 1226, 1351, 1369, 1734, 2694, 2884
Hpy99I CGWCG 8 cut(s) 44, 191, 194, 253, 271, 421, 478, 523
HpyAV CCTTC 7 cut(s) 996, 1058, 1706, 1792, 1859, 2002, 2119
HpyCH4III ACNGT 4 cut(s) 320, 1724, 2050, 2881
HpyCH4IV ACGT 2 cut(s) 618, 2416
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 208, 622, 2020
HpySE526I ACGT 2 cut(s) 618, 2416
Hsp92I GRCGYC 3 cut(s) 175, 248, 473
HspAI GCGC 4 cut(s) 175, 623, 719, 1451
KasI GGCGCC 1 cut(s) 174
Ksp22I TGATCA 2 cut(s) 652, 954
KspAI GTTAAC 1 cut(s) 1734
KspI CCGCGG 1 cut(s) 339
LmnI GCTCC 6 cut(s) 67, 86, 199, 914, 988, 994
Lsp1109I GCAGC 8 cut(s) 46, 181, 265, 408, 420, 1117, 2635, 2833
LweI GCATC 8 cut(s) 630, 916, 944, 1088, 1594, 2472, 2677, 2767
MaeI CTAG 2 cut(s) 2172, 2951
MaeII ACGT 2 cut(s) 618, 2416
MaeIII GTNAC 6 cut(s) 382, 820, 843, 1972, 2891, 2903
MfeI CAATTG 1 cut(s) 1860
MflI RGATCY 4 cut(s) 1555, 1632, 2175, 2336
MhlI GDGCHC 4 cut(s) 204, 507, 2076, 2082
MlsI TGGCCA 2 cut(s) 32, 816
MluNI TGGCCA 2 cut(s) 32, 816
Mly113I GGCGCC 1 cut(s) 175
MlyI GAGTC 4 cut(s) 233, 1108, 1634, 2834
MmeI TCCRAC 4 cut(s) 399, 1141, 1467, 2900
Mox20I TGGCCA 2 cut(s) 32, 816
Mph1103I ATGCAT 1 cut(s) 1212
MroXI GAANNNNTTC 1 cut(s) 2463
MscI TGGCCA 2 cut(s) 32, 816
MseI TTAA 8 cut(s) 861, 1467, 1478, 1733, 2060, 2328, 2387, 2762
MslI CAYNNNNRTG 1 cut(s) 2673
Msp20I TGGCCA 2 cut(s) 32, 816
MspA1I CMGCKG 4 cut(s) 54, 338, 433, 2648
MspCI CTTAAG 1 cut(s) 2386
MspI CCGG 3 cut(s) 380, 387, 965
MspR9I CCNGG 9 cut(s) 26, 291, 645, 818, 1426, 1988, 2181, 2487, 2794
MunI CAATTG 1 cut(s) 1860
Mva1269I GAATGC 1 cut(s) 2448
MvaI CCWGG 9 cut(s) 26, 291, 645, 818, 1426, 1988, 2181, 2487, 2794
MvnI CGCG 3 cut(s) 338, 721, 2571
MwoI GCNNNNNNNGC 4 cut(s) 21, 208, 622, 2020
NarI GGCGCC 1 cut(s) 175
NcoI CCATGG 4 cut(s) 33, 1086, 2401, 2661
NlaIV GGNNCC 9 cut(s) 176, 765, 916, 1276, 1389, 1430, 2148, 2177, 2659
NmeAIII GCCGAG 3 cut(s) 258, 297, 1096
NmuCI GTSAC 4 cut(s) 382, 1972, 2891, 2903
NsiI ATGCAT 1 cut(s) 1212
NspI RCATGY 2 cut(s) 12, 2689
NspV TTCGAA 5 cut(s) 2467, 2534, 2549, 2848, 2914
PagI TCATGA 2 cut(s) 259, 2230
PceI AGGCCT 1 cut(s) 314
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PctI GAATGC 1 cut(s) 2448
PdmI GAANNNNTTC 1 cut(s) 2463
PflMI CCANNNNNTGG 1 cut(s) 2186
PleI GAGTC 4 cut(s) 233, 1108, 1633, 2834
PluTI GGCGCC 1 cut(s) 178
PmaCI CACGTG 1 cut(s) 2417
PmlI CACGTG 1 cut(s) 2417
PpsI GAGTC 4 cut(s) 233, 1108, 1633, 2834
Ppu21I YACGTR 1 cut(s) 2417
PpuMI RGGWCCY 2 cut(s) 764, 1275
PsiI TTATAA 1 cut(s) 2582
Psp124BI GAGCTC 2 cut(s) 204, 2082
Psp5II RGGWCCY 2 cut(s) 764, 1275
Psp6I CCWGG 9 cut(s) 24, 289, 643, 816, 1424, 1986, 2179, 2485, 2792
PspCI CACGTG 1 cut(s) 2417
PspEI GGTNACC 1 cut(s) 382
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 9 cut(s) 24, 289, 643, 816, 1424, 1986, 2179, 2485, 2792
PspN4I GGNNCC 9 cut(s) 176, 765, 916, 1276, 1389, 1430, 2148, 2177, 2659
PspPI GGNCC 5 cut(s) 281, 764, 1275, 2590, 2962
PspPPI RGGWCCY 2 cut(s) 764, 1275
PstI CTGCAG 2 cut(s) 1693, 2653
PstNI CAGNNNCTG 1 cut(s) 2747
PsuI RGATCY 4 cut(s) 1555, 1632, 2175, 2336
PvuII CAGCTG 1 cut(s) 2648
RsaI GTAC 6 cut(s) 1227, 1938, 1960, 2008, 2543, 2632
RsaNI GTAC 6 cut(s) 1226, 1937, 1959, 2007, 2542, 2631
RseI CAYNNNNRTG 1 cut(s) 2673
SacI GAGCTC 2 cut(s) 204, 2082
SacII CCGCGG 1 cut(s) 339
SaqAI TTAA 8 cut(s) 861, 1467, 1478, 1733, 2060, 2328, 2387, 2762
Sau96I GGNCC 5 cut(s) 281, 764, 1275, 2590, 2962
ScaI AGTACT 2 cut(s) 2008, 2632
SchI GAGTC 4 cut(s) 233, 1108, 1634, 2834
ScrFI CCNGG 9 cut(s) 26, 291, 645, 818, 1426, 1988, 2181, 2487, 2794
SduI GDGCHC 4 cut(s) 204, 507, 2076, 2082
SfaNI GCATC 8 cut(s) 630, 916, 944, 1088, 1594, 2472, 2677, 2767
SfcI CTRYAG 7 cut(s) 319, 1245, 1689, 2374, 2622, 2643, 2649
SfoI GGCGCC 1 cut(s) 176
Sfr303I CCGCGG 1 cut(s) 339
SfuI TTCGAA 5 cut(s) 2467, 2534, 2549, 2848, 2914
SgrBI CCGCGG 1 cut(s) 339
SinI GGWCC 3 cut(s) 764, 1275, 2590
SmiMI CAYNNNNRTG 1 cut(s) 2673
SmlI CTYRAG 6 cut(s) 86, 167, 179, 1408, 2386, 2830
SmoI CTYRAG 6 cut(s) 86, 167, 179, 1408, 2386, 2830
SseBI AGGCCT 1 cut(s) 314
SspDI GGCGCC 1 cut(s) 174
SspI AATATT 2 cut(s) 1447, 2463
SspMI CTAG 2 cut(s) 2172, 2951
SstI GAGCTC 2 cut(s) 204, 2082
StuI AGGCCT 1 cut(s) 314
StyD4I CCNGG 9 cut(s) 24, 289, 643, 816, 1424, 1986, 2179, 2485, 2792
StyI CCWWGG 7 cut(s) 33, 1086, 1300, 2401, 2586, 2593, 2661
TaaI ACNGT 4 cut(s) 320, 1724, 2050, 2881
TaiI ACGT 2 cut(s) 621, 2419
TatI WGTACW 3 cut(s) 1225, 2006, 2630
TauI GCSGC 4 cut(s) 54, 175, 338, 433
Tru1I TTAA 8 cut(s) 861, 1467, 1478, 1733, 2060, 2328, 2387, 2762
Tru9I TTAA 8 cut(s) 861, 1467, 1478, 1733, 2060, 2328, 2387, 2762
TscAI CASTG 4 cut(s) 358, 576, 1566, 1672
TseFI GTSAC 4 cut(s) 382, 1972, 2891, 2903
TseI GCWGC 8 cut(s) 59, 194, 253, 396, 433, 1105, 2648, 2821
Tsp45I GTSAC 4 cut(s) 382, 1972, 2891, 2903
TspGWI ACGGA 4 cut(s) 255, 726, 1193, 2529
TspRI CASTG 4 cut(s) 358, 576, 1566, 1672
Van91I CCANNNNNTGG 1 cut(s) 2186
Vha464I CTTAAG 1 cut(s) 2386
VpaK11BI GGWCC 3 cut(s) 764, 1275, 2590
XapI RAATTY 3 cut(s) 1007, 1585, 1703
XceI RCATGY 2 cut(s) 12, 2689
XmnI GAANNNNTTC 1 cut(s) 2463
XspI CTAG 2 cut(s) 2172, 2951
ZrmI AGTACT 2 cut(s) 2008, 2632
Zsp2I ATGCAT 1 cut(s) 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.