Rorug06G0264100

NAD kinase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010084
Physical Location & Seq
Reverse (-)
41008308 .. 41012454
4147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0264100.1

Sequence Viewer

Length: 705 bp
ATGCTCTCCCTCGCGAAAAGACTGAAGGCGTTCCCTTGGAGCGTGGAGGCCGCCGGCAACCAACTCCGGCGATTACGAATGGAGGCCCGGTCTCCGAGGCGGCGGTCGAGGTCTCCGGCGCTGGCGATGAGGAAGGCGGAGGAGAAGTCGGAGTGGTGGATAGTCGACGGCGAAATGCACGAAATCGGCGACCACGTGCCGCCGCGTGAGCGGTTCGTGATTCCGAGAGATAACATCCCCAACAAGCGTCGGAAGCAGCTCAGGGAACAGTTCATGCGCCGCACTCGCCTCGTTCTCAAAGAAACGGAGCATGAGCCTTGGTGCAAAAAGTATATGGAGCTGTACCAGGAGCTTAGAGAGAATTGGGAAAGGCTGTACTGGGACGAAGGCTACTCCAAAAAGCTTGCCCAGGATCATGCGAATTACGAGTCAGCTGAAGATGATGATCAGGATTTCAATCCGTACCGGAGCAGGCAATCCCATTCCGAGCAATTTAAGGACCATGGATTTGGGAGTAGGCAAGGTGATACATGGGAAAAGGTTAACCAAATTAGGGATAAATTTGAGTATGACAGAGAAAGGAGGATGAAAGAAAAAGCATTTGCACCTATGCGTGGAGAAGTTTCTTCTAACGTCCATGATATGAACTCCAGAAGACAGCCATTTGACAGCCAGAGATACTTTTCCGACAGTGAAAGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

234

Amino Acids

28.59

Weight (kDa)

9.33

Isoelectric Point (pI)

68.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 211
AccI GTMKAC 1 cut(s) 165
AccII CGCG 2 cut(s) 14, 205
AciI CCGC 8 cut(s) 51, 100, 103, 137, 200, 203, 211, 280
AclWI GGATC 1 cut(s) 420
AcsI RAATTY 1 cut(s) 560
AcuI CTGAAG 2 cut(s) 44, 456
AcvI CACGTG 1 cut(s) 196
AfaI GTAC 3 cut(s) 344, 377, 464
AfiI CCNNNNNNNGG 2 cut(s) 553, 614
AgsI TTSAA 1 cut(s) 457
AjnI CCWGG 2 cut(s) 345, 408
AluBI AGCT 5 cut(s) 259, 340, 352, 403, 434
AluI AGCT 5 cut(s) 259, 340, 352, 403, 434
Alw26I GTCTC 2 cut(s) 96, 117
AlwI GGATC 1 cut(s) 420
AoxI GGCC 2 cut(s) 48, 84
ApeKI GCWGC 1 cut(s) 256
ApoI RAATTY 1 cut(s) 560
ArsI GACNNNNNNTTYG 4 cut(s) 413, 445, 491, 523
Asp700I GAANNNNTTC 1 cut(s) 29
AspLEI GCGC 2 cut(s) 121, 279
AspS9I GGNCC 2 cut(s) 85, 499
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 1 cut(s) 536
AvaII GGWCC 1 cut(s) 499
BbrPI CACGTG 1 cut(s) 196
BbsI GAAGAC 1 cut(s) 661
BbvI GCAGC 1 cut(s) 268
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciT130I CCWGG 2 cut(s) 347, 410
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 2 cut(s) 96, 117
BfoI RGCGCY 1 cut(s) 122
BisI GCNGC 6 cut(s) 51, 101, 200, 203, 257, 280
BlsI GCNGC 6 cut(s) 52, 102, 201, 204, 258, 281
Bme1390I CCNGG 3 cut(s) 88, 347, 410
Bme18I GGWCC 1 cut(s) 499
BmgT120I GGNCC 2 cut(s) 85, 499
BmrFI CCNGG 3 cut(s) 88, 347, 410
BmrI ACTGGG 1 cut(s) 388
BmuI ACTGGG 1 cut(s) 388
BpiI GAAGAC 1 cut(s) 661
BpmI CTGGAG 1 cut(s) 634
Bpu10I CCTNAGC 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 88
BsaAI YACGTR 1 cut(s) 196
BsaBI GATNNNNATC 2 cut(s) 444, 456
BsaI GGTCTC 2 cut(s) 96, 117
BsaJI CCNNGG 5 cut(s) 35, 95, 317, 408, 502
BsaWI WCCGGW 1 cut(s) 465
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 131, 161
Bsc4I CCNNNNNNNGG 2 cut(s) 553, 614
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 383
Bse8I GATNNNNATC 2 cut(s) 444, 456
BseBI CCWGG 2 cut(s) 347, 410
BseDI CCNNGG 5 cut(s) 35, 95, 317, 408, 502
BseGI GGATG 2 cut(s) 234, 591
BseJI GATNNNNATC 2 cut(s) 444, 456
BseLI CCNNNNNNNGG 2 cut(s) 553, 614
BseMII CTCAG 1 cut(s) 274
BseNI ACTGG 1 cut(s) 383
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 268
Bsh1236I CGCG 2 cut(s) 14, 205
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 2 cut(s) 50, 86
BsiEI CGRYCG 1 cut(s) 107
BsiSI CCGG 5 cut(s) 54, 67, 88, 116, 466
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 2 cut(s) 553, 614
BsmAI GTCTC 2 cut(s) 96, 117
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 2 cut(s) 50, 86
Bso31I GGTCTC 2 cut(s) 96, 117
Bsp143I GATC 2 cut(s) 412, 445
Bsp19I CCATGG 1 cut(s) 502
Bsp68I TCGCGA 1 cut(s) 14
BspACI CCGC 8 cut(s) 51, 100, 103, 137, 200, 203, 211, 280
BspANI GGCC 2 cut(s) 50, 86
BspCNI CTCAG 1 cut(s) 273
BspFNI CGCG 2 cut(s) 14, 205
BspPI GGATC 1 cut(s) 420
BspTNI GGTCTC 2 cut(s) 96, 117
BsrBI CCGCTC 1 cut(s) 211
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 1 cut(s) 383
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 5 cut(s) 35, 95, 317, 408, 502
BssMI GATC 2 cut(s) 412, 445
BssT1I CCWWGG 3 cut(s) 35, 317, 502
Bst2UI CCWGG 2 cut(s) 347, 410
Bst4CI ACNGT 2 cut(s) 270, 692
BstBAI YACGTR 1 cut(s) 196
BstC8I GCNNGC 4 cut(s) 55, 123, 405, 473
BstDEI CTNAG 2 cut(s) 260, 353
BstDSI CCRYGG 1 cut(s) 502
BstF5I GGATG 2 cut(s) 234, 591
BstFNI CGCG 2 cut(s) 14, 205
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 2 cut(s) 121, 279
BstKTI GATC 2 cut(s) 415, 448
BstMAI GTCTC 2 cut(s) 96, 117
BstMBI GATC 2 cut(s) 412, 445
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 3 cut(s) 208, 253, 285
BstNI CCWGG 2 cut(s) 347, 410
BstSCI CCNGG 3 cut(s) 86, 345, 408
BstUI CGCG 2 cut(s) 14, 205
BstV1I GCAGC 1 cut(s) 268
BstV2I GAAGAC 1 cut(s) 661
BstXI CCANNNNNNTGG 1 cut(s) 509
BsuRI GGCC 2 cut(s) 50, 86
BtgI CCRYGG 1 cut(s) 502
BtgZI GCGATG 1 cut(s) 140
BtsCI GGATG 2 cut(s) 234, 591
BtsIMutI CAGTG 1 cut(s) 697
BtuMI TCGCGA 1 cut(s) 14
Cac8I GCNNGC 4 cut(s) 55, 123, 405, 473
CfoI GCGC 2 cut(s) 121, 279
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 85, 499
CseI GACGC 1 cut(s) 236
Csp6I GTAC 3 cut(s) 343, 376, 463
CviAII CATG 6 cut(s) 274, 311, 416, 503, 531, 638
CviQI GTAC 3 cut(s) 343, 376, 463
DdeI CTNAG 2 cut(s) 260, 353
DpnI GATC 2 cut(s) 414, 447
DpnII GATC 2 cut(s) 412, 445
EciI GGCGGA 1 cut(s) 152
Eco130I CCWWGG 3 cut(s) 35, 317, 502
Eco31I GGTCTC 2 cut(s) 96, 117
Eco47I GGWCC 1 cut(s) 499
Eco57I CTGAAG 2 cut(s) 44, 456
Eco72I CACGTG 1 cut(s) 196
EcoRII CCWGG 2 cut(s) 345, 408
EcoT14I CCWWGG 3 cut(s) 35, 317, 502
ErhI CCWWGG 3 cut(s) 35, 317, 502
FaeI CATG 6 cut(s) 277, 314, 419, 506, 534, 641
FaqI GGGAC 1 cut(s) 395
FatI CATG 6 cut(s) 273, 310, 415, 502, 530, 637
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 6 cut(s) 51, 101, 200, 203, 257, 280
FokI GGATG 2 cut(s) 221, 598
Fsp4HI GCNGC 6 cut(s) 51, 101, 200, 203, 257, 280
GlaI GCGC 2 cut(s) 120, 278
GluI GCNGC 6 cut(s) 51, 101, 200, 203, 257, 280
GsuI CTGGAG 1 cut(s) 634
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 2 cut(s) 50, 86
HapII CCGG 5 cut(s) 54, 67, 88, 116, 466
HgaI GACGC 1 cut(s) 236
HhaI GCGC 2 cut(s) 121, 279
Hin1II CATG 6 cut(s) 277, 314, 419, 506, 534, 641
Hin6I GCGC 2 cut(s) 119, 277
HinP1I GCGC 2 cut(s) 119, 277
HincII GTYRAC 2 cut(s) 166, 544
HindII GTYRAC 2 cut(s) 166, 544
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 2 cut(s) 220, 428
HpaI GTTAAC 1 cut(s) 544
HpaII CCGG 5 cut(s) 54, 67, 88, 116, 466
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 2 cut(s) 166, 544
Hpy188I TCNGA 6 cut(s) 96, 151, 225, 252, 487, 688
Hpy188III TCNNGA 4 cut(s) 13, 217, 449, 651
Hpy8I GTNNAC 2 cut(s) 166, 544
Hpy99I CGWCG 2 cut(s) 170, 252
HpyAV CCTTC 3 cut(s) 19, 127, 380
HpyCH4III ACNGT 2 cut(s) 270, 692
HpyCH4IV ACGT 2 cut(s) 195, 633
HpyCH4V TGCA 3 cut(s) 178, 324, 605
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 253, 285
HpyF3I CTNAG 2 cut(s) 260, 353
HpySE526I ACGT 2 cut(s) 195, 633
Hsp92II CATG 6 cut(s) 277, 314, 419, 506, 534, 641
HspAI GCGC 2 cut(s) 119, 277
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 445
KspAI GTTAAC 1 cut(s) 544
Kzo9I GATC 2 cut(s) 412, 445
LmnI GCTCC 5 cut(s) 39, 307, 337, 349, 468
Lsp1109I GCAGC 1 cut(s) 268
MaeII ACGT 2 cut(s) 195, 633
MalI GATC 2 cut(s) 414, 447
MbiI CCGCTC 1 cut(s) 211
MboI GATC 2 cut(s) 412, 445
MboII GAAGA 3 cut(s) 449, 618, 666
MluCI AATT 5 cut(s) 361, 421, 491, 549, 560
MlyI GAGTC 1 cut(s) 437
MmeI TCCRAC 2 cut(s) 129, 230
MnlI CCTC 9 cut(s) 20, 40, 76, 90, 102, 123, 133, 299, 576
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 29
MseI TTAA 2 cut(s) 495, 543
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 5 cut(s) 54, 67, 88, 116, 466
MspR9I CCNGG 3 cut(s) 88, 347, 410
MvaI CCWGG 2 cut(s) 347, 410
MvnI CGCG 2 cut(s) 14, 205
MwoI GCNNNNNNNGC 3 cut(s) 208, 253, 285
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 88
NcoI CCATGG 1 cut(s) 502
NdeII GATC 2 cut(s) 412, 445
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 6 cut(s) 277, 314, 419, 506, 534, 641
NruI TCGCGA 1 cut(s) 14
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 192
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 29
PfeI GAWTC 1 cut(s) 220
PkrI GCNGC 6 cut(s) 52, 102, 201, 204, 258, 281
PleI GAGTC 1 cut(s) 436
PmaCI CACGTG 1 cut(s) 196
PmlI CACGTG 1 cut(s) 196
PpsI GAGTC 1 cut(s) 436
Ppu21I YACGTR 1 cut(s) 196
Psp6I CCWGG 2 cut(s) 345, 408
PspCI CACGTG 1 cut(s) 196
PspGI CCWGG 2 cut(s) 345, 408
PspPI GGNCC 2 cut(s) 85, 499
PvuII CAGCTG 1 cut(s) 434
RruI TCGCGA 1 cut(s) 14
RsaI GTAC 3 cut(s) 344, 377, 464
RsaNI GTAC 3 cut(s) 343, 376, 463
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 2 cut(s) 495, 543
SatI GCNGC 6 cut(s) 51, 101, 200, 203, 257, 280
Sau3AI GATC 2 cut(s) 412, 445
Sau96I GGNCC 2 cut(s) 85, 499
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 3 cut(s) 88, 347, 410
SinI GGWCC 1 cut(s) 499
Sse9I AATT 5 cut(s) 361, 421, 491, 549, 560
SsiI CCGC 8 cut(s) 51, 100, 103, 137, 200, 203, 211, 280
StyD4I CCNGG 3 cut(s) 86, 345, 408
StyI CCWWGG 3 cut(s) 35, 317, 502
TaaI ACNGT 2 cut(s) 270, 692
TaiI ACGT 2 cut(s) 198, 636
TaqI TCGA 2 cut(s) 107, 165
TasI AATT 5 cut(s) 361, 421, 491, 549, 560
TatI WGTACW 1 cut(s) 375
TauI GCSGC 5 cut(s) 53, 103, 202, 205, 282
TfiI GAWTC 1 cut(s) 220
Tru1I TTAA 2 cut(s) 495, 543
Tru9I TTAA 2 cut(s) 495, 543
TscAI CASTG 1 cut(s) 697
TseI GCWGC 1 cut(s) 256
TspDTI ATGAA 3 cut(s) 262, 602, 659
TspGWI ACGGA 2 cut(s) 320, 450
TspRI CASTG 1 cut(s) 697
VpaK11BI GGWCC 1 cut(s) 499
XapI RAATTY 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.