Rw6G032770

NAD kinase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010084
Physical Location & Seq
Reverse (-)
57016086 .. 57020854
4769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032770.1

Sequence Viewer

Length: 2724 bp
ATGGTGGCATGTTGCCTCATGTGCCAGGTGGCCATCGCCGTCGATATGAGCCGCTGTGCCGCTTCACCTCCCAATCTGTGCCCCCTCCAGTTCTCAGGTAGCCCCGCGAAGCTATTCGGATTCGGGTTCGACATTCAGAAGAAGGACCGGCTCAAGAGGCGGCTCAAGTTCGTCGTCAGTGCGGAGCTGTCCAAGCCATTCTCTCTCGGCTTCGGCTTGGACTCTCAGGGGTATCAGCTTGACGATCCGTCGCAGTCGCGGAGGATTGGCCCTGTTCCTGGGGATATTGCAGAAATTGAAGCCTATTGTAGAATTTTTCGAAGCGCTGAACGACTGCATACTGCATTGATGGAGACATTGTGCAATCCGGTGACCGGGGTGTGCAGTGTTTATTATGACTTCCCATCGGAGGAAAAGCCGCTCTTGGAGGATAAGATTGTGTCTGTGATTGGTTGCATGATGTCACTGTTGAACAAAGCAAGGGAGGATGTGCTTTCCGGAAGATCAGCCATTATGAGCTCCTTTCGAGGAGTTGTGGATGTCGCTCTTCCTCCACTTGCTGTTTTCAGGAGTGAGCTGAAGAGGTGTTGCGAGAGCTTGCACGTTGCGCTTGAGGACTGGTTGATGCCTGGGGATGACCAGAGCTTGGATGTGTGGAGGAAACTTCAGAGGCTCAAGAATGTGTGTTATGATTCCGGTTTTCCACGCCAGGAGGATTATCCGTGCAATACACTTTTTGCTAATTGGACTCCTGTTTACTTATCCTCTTCTAAAGAGGATGTTAGGTCCGAAGATTCAGAGGTAGCGTTTTGGAGGGGTGGCCAGGTAACAGAAGAAGGTCTGGACTGGTTACTGGAGAAGGGATTTAAAACTCTTGTTGATCTTAGGGCAGAGATTGTAAAAGACAATATTTATCAGGCAGCCCTAGATGATGCTATTGCATCAGGGAAGGTGGAATTGATCAGAATTCCAGTTGAAGTTGGGACTGCACCTTCAATGGAGCAGGTTGAGAAATTCGCAAGTTTGGTTTCAGATTGCAGCAAAAAGCCAATCTATCTTCATAGCAAGGAAGGTCTCTGGAGAACATCTGCCATGGTTTCCAGATGGAGGCAGCACTTGACTCGTCGTGCTGTTCAGTTTGTCTCCAACCAGTCAGTTTCTCTTAATGGAGAAGTTGAAAAACCCTCAACTATGCAGAAAAGTTCCCTCCTTGAAAAGAAGAATGAATCACTGGACACAATTCATGGTTCAAATGGGTTGCTACAAAAAAATGATTCTGTAGACAGGGATGAAGCAAATCTAAATGGGACCTGCAATGGCCTTATATCTATCCAAGGTTTGACATCAGTTGAATCAGATGAAAGTGGGGAGAAACCTTTGGTGAACTTCTGCAGGGAAGTTGACCCCCTGAATGCTCAGGTTCCTCCTTGCAATATTTTCTCAAGAAAAGAAATGTCCAGGTTTCTTGAGAGAAAAAATATTGCACCACTCACTTATTTTAATTATCAGTTAAATAGGTTGAAGGTATTACCAATTTCCAAATATATGAATACTAAGATAATGTGGAGAGGTGAGATTGTTGATACTGATTCAGTGCGGGAGGTTGTGGAGGCAGAAAATTCCAATGGGATACCTGATGCAGAACATCTGCTTCCAGAGTTCCAGATCTCTGCTTCTGGCAATGGGAAGTATTTGACCGGTATTGGTAATGGTTCTGTAACTGCAGTCGTAAATGGATATGGTGAAGGGGAGAAAGGTTCCTTGTTAGCCACTAATTTATCTACTAACGCGAGCAACAATCAATCTGAGAGTGTCTTATCTAAAGCAGTGAAGGAGCAAAGGAAGAGTAATGGTAGAGCCCCTTTAGTTTCAAGTGATGATGAATTGGGATCAATTGAAGGAGATATGTGTGCTTCTGCAACTGGGGTTGTAAGGGTGCAGTCAAGAAAGAAAGCAGAGATGTTTTTAGTACGAACAGATGGATTCTCTTGTACCCGAGAAAAAGTGACAGAATCCTCCCTGGCCTTCACTCATCCAAGTACTCAGCAACAAATGCTTATGTGGAAATCTACACCAAAGACAGTATTAGTGTTAAAGAAGCTTGGGCATGAGCTTATGGAACAAGCTAAAGAGGTTGTCTCCTTCTTGTATTACCAAGAGAAAATGAATGTTCTGGTGGAACCTGACGTGCATGATGTATTTGCTCGAATCCCAGGTTTTGGATTTGTTCAGACCTTCTACACCCAAGATACCGGTGATCTGCATGAGAGGGTTGATTTTGTGGCATGTCTGGGAGGGGATGGGGTAATACTCCATGCTTCAAATCTATTCAAAGGTGCTGTTCCCCCCATCGTATCATTTAATCTTGGATCTCTTGGATTTTTGACTTCCCATACTTTTGATGACTACAGGCAGGACTTAAGACAGGTTATCCATGGAAATAACACGAGTGATGGTGTTTATATAACTCTCAGAATGCGTCTCAGGTGTGAAATTTTTCGAAATGGTAAAGCGATGCCTGGAAAGGTATTTGATGTTTTGAATGAAATAGTTGTTGATCGAGGTTCGAATCCGTACCTTTCTAAGATTGAATGTTATGAACGTGACCAGCTTATAACCAAGGTCCAAGGTGATGGTGTCATTGTAGCCACACCTACAGGAAGTACTGCTTATTCTACAGCTGCAGGAGGTTCCATGGTCAGAAAATGCTTTTTAGTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

907

Amino Acids

100.56

Weight (kDa)

5.89

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-NADK PF22741 241 - 387 3.4e-64 DSP-PTPase phosphatase fused to NAD+ Kinase
Tc-R-P PF22785 275 - 369 5e-11 Polymorphic toxin system, DSP-PTPase phosphatase
NAD_kinase PF01513 703 - 813 4e-15 ATP-NAD kinase N-terminal domain
NAD_kinase_C PF20143 847 - 901 8.5e-13 ATP-NAD kinase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2615
Acc36I ACCTGC 2 cut(s) 994, 1319
AccB7I CCANNNNNTGG 2 cut(s) 646, 2219
AccBSI CCGCTC 1 cut(s) 421
AccI GTMKAC 1 cut(s) 1281
AccII CGCG 3 cut(s) 107, 259, 1790
AccIII TCCGGA 1 cut(s) 497
AciI CCGC 8 cut(s) 52, 60, 105, 160, 182, 259, 419, 1597
AclWI GGATC 3 cut(s) 239, 1897, 2377
AcoI YGGCCR 2 cut(s) 30, 820
AcsI RAATTY 5 cut(s) 312, 966, 1013, 1618, 2493
AcuI CTGAAG 2 cut(s) 599, 650
AfaI GTAC 5 cut(s) 1971, 1993, 2041, 2576, 2665
AfeI AGCGCT 1 cut(s) 325
AfiI CCNNNNNNNGG 6 cut(s) 278, 374, 409, 646, 1107, 2219
AflII CTTAAG 1 cut(s) 2419
AgeI ACCGGT 2 cut(s) 1697, 2252
AhdI GACNNNNNGTC 1 cut(s) 247
AjiI CACGTC 1 cut(s) 2188
AjnI CCWGG 9 cut(s) 24, 277, 628, 708, 822, 1457, 2019, 2212, 2518
Alw21I GWGCWC 1 cut(s) 521
Alw26I GTCTC 5 cut(s) 347, 1079, 1147, 2143, 2486
AlwI GGATC 3 cut(s) 239, 1897, 2377
Ama87I CYCGRG 1 cut(s) 1995
Aor13HI TCCGGA 1 cut(s) 497
Aor51HI AGCGCT 1 cut(s) 325
AoxI GGCC 5 cut(s) 30, 268, 820, 1318, 2022
ApeKI GCWGC 4 cut(s) 920, 1038, 1111, 2681
ApoI RAATTY 5 cut(s) 312, 966, 1013, 1618, 2493
AsiGI ACCGGT 2 cut(s) 1697, 2252
Asp700I GAANNNNTTC 2 cut(s) 113, 2496
AspLEI GCGC 2 cut(s) 326, 610
AspS9I GGNCC 5 cut(s) 145, 269, 786, 1308, 2623
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 7 cut(s) 57, 382, 1393, 1583, 1754, 2267, 2642
AsuII TTCGAA 3 cut(s) 319, 2500, 2567
AvaI CYCGRG 1 cut(s) 1995
AvaII GGWCC 4 cut(s) 145, 786, 1308, 2623
BaeGI GKGCMC 1 cut(s) 83
BalI TGGCCA 2 cut(s) 32, 822
BanII GRGCYC 2 cut(s) 521, 1861
BauI CACGAG 1 cut(s) 2446
Bbv12I GWGCWC 1 cut(s) 521
BbvI GCAGC 4 cut(s) 932, 1050, 1123, 2668
BccI CCATC 9 cut(s) 41, 343, 412, 1098, 1973, 2294, 2357, 2447, 2627
BceAI ACGGC 1 cut(s) 23
BcgI CGANNNNNNTGC 2 cut(s) 1103, 1137
BciT130I CCWGG 9 cut(s) 26, 279, 630, 710, 824, 1459, 2021, 2214, 2520
BciVI GTATCC 1 cut(s) 1623
BclI TGATCA 1 cut(s) 960
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 5 cut(s) 347, 1079, 1147, 2143, 2486
BfaI CTAG 1 cut(s) 926
BfmI CTRYAG 7 cut(s) 1278, 1390, 1722, 2407, 2655, 2676, 2682
BfoI RGCGCY 1 cut(s) 327
BfrI CTTAAG 1 cut(s) 2419
BfuAI ACCTGC 2 cut(s) 994, 1319
BfuI GTATCC 1 cut(s) 1623
BglII AGATCT 1 cut(s) 1665
BisI GCNGC 8 cut(s) 52, 60, 161, 419, 921, 1039, 1112, 2682
BlsI GCNGC 8 cut(s) 53, 61, 162, 420, 922, 1040, 1113, 2683
BmcAI AGTACT 2 cut(s) 2041, 2665
Bme18I GGWCC 4 cut(s) 145, 786, 1308, 2623
BmeRI GACNNNNNGTC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 1995
BmgBI CACGTC 1 cut(s) 2188
BmgT120I GGNCC 5 cut(s) 145, 269, 786, 1308, 2623
BmiI GGNNCC 5 cut(s) 1309, 1422, 1759, 2181, 2692
BmrI ACTGGG 1 cut(s) 1932
BmsI GCATC 5 cut(s) 615, 922, 950, 1627, 2505
BmuI ACTGGG 1 cut(s) 1932
BplI GAGNNNNNCTC 2 cut(s) 2123, 2155
BpmI CTGGAG 3 cut(s) 71, 875, 1099
Bpu10I CCTNAGC 1 cut(s) 1416
Bpu14I TTCGAA 3 cut(s) 319, 2500, 2567
BpuEI CTTGAG 6 cut(s) 137, 149, 632, 659, 1426, 1487
BpuMI CCSGG 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 1079
BsaWI WCCGGW 5 cut(s) 367, 497, 695, 1697, 2252
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 6 cut(s) 278, 374, 409, 646, 1107, 2219
Bse118I RCCGGY 3 cut(s) 147, 1697, 2252
Bse1I ACTGG 8 cut(s) 88, 623, 851, 858, 971, 1150, 1236, 1927
Bse3DI GCAATG 2 cut(s) 1321, 1687
BseAI TCCGGA 1 cut(s) 497
BseBI CCWGG 9 cut(s) 26, 279, 630, 710, 824, 1459, 2021, 2214, 2520
BseGI GGATG 8 cut(s) 493, 544, 640, 655, 784, 1294, 2032, 2305
BseLI CCNNNNNNNGG 6 cut(s) 278, 374, 409, 646, 1107, 2219
BseMI GCAATG 2 cut(s) 1321, 1687
BseMII CTCAG 7 cut(s) 108, 239, 1430, 1797, 2057, 2485, 2497
BseNI ACTGG 8 cut(s) 88, 623, 851, 858, 971, 1150, 1236, 1927
BseRI GAGGAG 1 cut(s) 543
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 4 cut(s) 932, 1050, 1123, 2668
BsgI GTGCAG 3 cut(s) 403, 972, 1958
Bsh1236I CGCG 3 cut(s) 107, 259, 1790
BshFI GGCC 5 cut(s) 32, 270, 822, 1320, 2024
BshTI ACCGGT 2 cut(s) 1697, 2252
BsiHKAI GWGCWC 1 cut(s) 521
BsiHKCI CYCGRG 1 cut(s) 1995
BsiSI CCGG 7 cut(s) 148, 368, 375, 498, 696, 1698, 2253
BslFI GGGAC 2 cut(s) 997, 1321
BslI CCNNNNNNNGG 6 cut(s) 278, 374, 409, 646, 1107, 2219
BsmAI GTCTC 5 cut(s) 347, 1079, 1147, 2143, 2486
BsmBI CGTCTC 1 cut(s) 2486
BsmFI GGGAC 2 cut(s) 997, 1321
BsmI GAATGC 2 cut(s) 1417, 2481
BsnI GGCC 5 cut(s) 32, 270, 822, 1320, 2024
Bso31I GGTCTC 1 cut(s) 1079
BsoBI CYCGRG 1 cut(s) 1995
Bsp119I TTCGAA 3 cut(s) 319, 2500, 2567
Bsp1286I GDGCHC 3 cut(s) 83, 521, 1861
Bsp13I TCCGGA 1 cut(s) 497
Bsp143I GATC 9 cut(s) 244, 503, 880, 960, 1665, 1889, 2257, 2369, 2557
Bsp19I CCATGG 3 cut(s) 1092, 2434, 2694
BspACI CCGC 8 cut(s) 52, 60, 105, 160, 182, 259, 419, 1597
BspANI GGCC 5 cut(s) 32, 270, 822, 1320, 2024
BspCNI CTCAG 7 cut(s) 107, 238, 1429, 1798, 2056, 2484, 2496
BspEI TCCGGA 1 cut(s) 497
BspFNI CGCG 3 cut(s) 107, 259, 1790
BspLI GGNNCC 5 cut(s) 1309, 1422, 1759, 2181, 2692
BspMAI CTGCAG 3 cut(s) 1394, 1726, 2686
BspMI ACCTGC 2 cut(s) 994, 1319
BspPI GGATC 3 cut(s) 239, 1897, 2377
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 3 cut(s) 319, 2500, 2567
BspTI CTTAAG 1 cut(s) 2419
BspTNI GGTCTC 1 cut(s) 1079
BsrBI CCGCTC 1 cut(s) 421
BsrDI GCAATG 2 cut(s) 1321, 1687
BsrFI RCCGGY 3 cut(s) 147, 1697, 2252
BsrI ACTGG 8 cut(s) 88, 623, 851, 858, 971, 1150, 1236, 1927
BssAI RCCGGY 3 cut(s) 147, 1697, 2252
BssMI GATC 9 cut(s) 244, 503, 880, 960, 1665, 1889, 2257, 2369, 2557
BssSI CACGAG 1 cut(s) 2446
BssT1I CCWWGG 6 cut(s) 1092, 1333, 2434, 2619, 2626, 2694
Bst2BI CACGAG 1 cut(s) 2446
Bst2UI CCWGG 9 cut(s) 26, 279, 630, 710, 824, 1459, 2021, 2214, 2520
Bst4CI ACNGT 2 cut(s) 468, 2083
Bst6I CTCTTC 4 cut(s) 552, 575, 772, 1838
BstAFI CTTAAG 1 cut(s) 2419
BstAPI GCANNNNNTGC 1 cut(s) 2053
BstBI TTCGAA 3 cut(s) 319, 2500, 2567
BstC8I GCNNGC 2 cut(s) 599, 1792
BstDSI CCRYGG 3 cut(s) 1092, 2434, 2694
BstEII GGTNACC 1 cut(s) 370
BstF5I GGATG 8 cut(s) 493, 544, 640, 655, 784, 1294, 2032, 2305
BstFNI CGCG 3 cut(s) 107, 259, 1790
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 2 cut(s) 326, 610
BstKTI GATC 9 cut(s) 247, 506, 883, 963, 1668, 1892, 2260, 2372, 2560
BstMAI GTCTC 5 cut(s) 347, 1079, 1147, 2143, 2486
BstMBI GATC 9 cut(s) 244, 503, 880, 960, 1665, 1889, 2257, 2369, 2557
BstMWI GCNNNNNNNGC 5 cut(s) 21, 157, 193, 607, 2053
BstNI CCWGG 9 cut(s) 26, 279, 630, 710, 824, 1459, 2021, 2214, 2520
BstNSI RCATGY 2 cut(s) 12, 2289
BstPI GGTNACC 1 cut(s) 370
BstSFI CTRYAG 7 cut(s) 1278, 1390, 1722, 2407, 2655, 2676, 2682
BstSLI GKGCMC 1 cut(s) 83
BstUI CGCG 3 cut(s) 107, 259, 1790
BstV1I GCAGC 4 cut(s) 932, 1050, 1123, 2668
BstX2I RGATCY 2 cut(s) 1665, 2369
BstXI CCANNNNNNTGG 1 cut(s) 2633
BstYI RGATCY 2 cut(s) 1665, 2369
BsuI GTATCC 1 cut(s) 1623
BsuRI GGCC 5 cut(s) 32, 270, 822, 1320, 2024
BtgI CCRYGG 3 cut(s) 1092, 2434, 2694
BtgZI GCGATG 2 cut(s) 19, 2528
BtrI CACGTC 1 cut(s) 2188
BtsCI GGATG 8 cut(s) 493, 544, 640, 655, 784, 1294, 2032, 2305
BtsI GCAGTG 2 cut(s) 391, 1833
BtsIMutI CAGTG 6 cut(s) 184, 391, 464, 1229, 1599, 1833
BveI ACCTGC 2 cut(s) 994, 1319
Cac8I GCNNGC 2 cut(s) 599, 1792
CfoI GCGC 2 cut(s) 326, 610
Cfr10I RCCGGY 3 cut(s) 147, 1697, 2252
Cfr13I GGNCC 5 cut(s) 145, 269, 786, 1308, 2623
CseI GACGC 1 cut(s) 2468
Csp6I GTAC 5 cut(s) 1970, 1992, 2040, 2575, 2664
CspAI ACCGGT 2 cut(s) 1697, 2252
CviQI GTAC 5 cut(s) 1970, 1992, 2040, 2575, 2664
DpnI GATC 9 cut(s) 246, 505, 882, 962, 1667, 1891, 2259, 2371, 2559
DpnII GATC 9 cut(s) 244, 503, 880, 960, 1665, 1889, 2257, 2369, 2557
DraI TTTAAA 1 cut(s) 868
DriI GACNNNNNGTC 1 cut(s) 247
EaeI YGGCCR 2 cut(s) 30, 820
Eam1104I CTCTTC 4 cut(s) 552, 575, 772, 1838
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 4 cut(s) 552, 575, 772, 1838
Ecl136II GAGCTC 1 cut(s) 519
Eco130I CCWWGG 6 cut(s) 1092, 1333, 2434, 2619, 2626, 2694
Eco24I GRGCYC 2 cut(s) 521, 1861
Eco31I GGTCTC 1 cut(s) 1079
Eco47I GGWCC 4 cut(s) 145, 786, 1308, 2623
Eco47III AGCGCT 1 cut(s) 325
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 2 cut(s) 599, 650
Eco88I CYCGRG 1 cut(s) 1995
Eco91I GGTNACC 1 cut(s) 370
EcoICRI GAGCTC 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 1308
EcoO65I GGTNACC 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 966
EcoRII CCWGG 9 cut(s) 24, 277, 628, 708, 822, 1457, 2019, 2212, 2518
EcoT14I CCWWGG 6 cut(s) 1092, 1333, 2434, 2619, 2626, 2694
EcoT38I GRGCYC 2 cut(s) 521, 1861
ErhI CCWWGG 6 cut(s) 1092, 1333, 2434, 2619, 2626, 2694
Esp3I CGTCTC 1 cut(s) 2486
FalI AAGNNNNNCTT 4 cut(s) 407, 439, 2653, 2685
FaqI GGGAC 2 cut(s) 997, 1321
FauI CCCGC 2 cut(s) 112, 1590
FbaI TGATCA 1 cut(s) 960
FblI GTMKAC 1 cut(s) 1281
Fnu4HI GCNGC 8 cut(s) 52, 60, 161, 419, 921, 1039, 1112, 2682
FokI GGATG 8 cut(s) 500, 551, 647, 662, 791, 1301, 2019, 2312
FriOI GRGCYC 2 cut(s) 521, 1861
Fsp4HI GCNGC 8 cut(s) 52, 60, 161, 419, 921, 1039, 1112, 2682
FspBI CTAG 1 cut(s) 926
GlaI GCGC 2 cut(s) 325, 609
GluI GCNGC 8 cut(s) 52, 60, 161, 419, 921, 1039, 1112, 2682
GsuI CTGGAG 3 cut(s) 71, 875, 1099
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 5 cut(s) 32, 270, 822, 1320, 2024
HapII CCGG 7 cut(s) 148, 368, 375, 498, 696, 1698, 2253
HgaI GACGC 1 cut(s) 2468
HhaI GCGC 2 cut(s) 326, 610
Hin6I GCGC 2 cut(s) 324, 608
HinP1I GCGC 2 cut(s) 324, 608
HincII GTYRAC 1 cut(s) 1402
HindII GTYRAC 1 cut(s) 1402
HindIII AAGCTT 1 cut(s) 2099
HpaII CCGG 7 cut(s) 148, 368, 375, 498, 696, 1698, 2253
HphI GGTGA 7 cut(s) 57, 382, 1393, 1583, 1754, 2267, 2642
Hpy166II GTNNAC 4 cut(s) 757, 1282, 1384, 1402
Hpy8I GTNNAC 4 cut(s) 757, 1282, 1384, 1402
Hpy99I CGWCG 4 cut(s) 44, 176, 253, 1128
HpyCH4III ACNGT 2 cut(s) 468, 2083
HpyCH4IV ACGT 3 cut(s) 603, 2187, 2602
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 157, 193, 607, 2053
HpySE526I ACGT 3 cut(s) 603, 2187, 2602
HspAI GCGC 2 cut(s) 324, 608
Kpn2I TCCGGA 1 cut(s) 497
Ksp22I TGATCA 1 cut(s) 960
Kzo9I GATC 9 cut(s) 244, 503, 880, 960, 1665, 1889, 2257, 2369, 2557
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 4 cut(s) 184, 524, 1000, 1834
Lsp1109I GCAGC 4 cut(s) 932, 1050, 1123, 2668
LweI GCATC 5 cut(s) 615, 922, 950, 1627, 2505
MaeI CTAG 1 cut(s) 926
MaeII ACGT 3 cut(s) 603, 2187, 2602
MaeIII GTNAC 7 cut(s) 370, 462, 826, 849, 1717, 2005, 2603
MalI GATC 9 cut(s) 246, 505, 882, 962, 1667, 1891, 2259, 2371, 2559
MbiI CCGCTC 1 cut(s) 421
MboI GATC 9 cut(s) 244, 503, 880, 960, 1665, 1889, 2257, 2369, 2557
MfeI CAATTG 1 cut(s) 1893
MflI RGATCY 2 cut(s) 1665, 2369
MhlI GDGCHC 3 cut(s) 83, 521, 1861
MlsI TGGCCA 2 cut(s) 32, 822
MluNI TGGCCA 2 cut(s) 32, 822
MlyI GAGTC 3 cut(s) 215, 742, 1114
MmeI TCCRAC 1 cut(s) 1170
Mox20I TGGCCA 2 cut(s) 32, 822
MroI TCCGGA 1 cut(s) 497
MroXI GAANNNNTTC 2 cut(s) 113, 2496
MscI TGGCCA 2 cut(s) 32, 822
MseI TTAA 7 cut(s) 867, 1164, 1500, 1511, 2093, 2361, 2420
Msp20I TGGCCA 2 cut(s) 32, 822
MspA1I CMGCKG 2 cut(s) 54, 2681
MspCI CTTAAG 1 cut(s) 2419
MspI CCGG 7 cut(s) 148, 368, 375, 498, 696, 1698, 2253
MunI CAATTG 1 cut(s) 1893
Mva1269I GAATGC 2 cut(s) 1417, 2481
MvaI CCWGG 9 cut(s) 26, 279, 630, 710, 824, 1459, 2021, 2214, 2520
MvnI CGCG 3 cut(s) 107, 259, 1790
MwoI GCNNNNNNNGC 5 cut(s) 21, 157, 193, 607, 2053
NciI CCSGG 1 cut(s) 376
NcoI CCATGG 3 cut(s) 1092, 2434, 2694
NdeII GATC 9 cut(s) 244, 503, 880, 960, 1665, 1889, 2257, 2369, 2557
NlaIV GGNNCC 5 cut(s) 1309, 1422, 1759, 2181, 2692
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 4 cut(s) 370, 462, 2005, 2603
NspI RCATGY 2 cut(s) 12, 2289
NspV TTCGAA 3 cut(s) 319, 2500, 2567
PciSI GCTCTTC 1 cut(s) 552
PctI GAATGC 2 cut(s) 1417, 2481
PdmI GAANNNNTTC 2 cut(s) 113, 2496
PflMI CCANNNNNTGG 2 cut(s) 646, 2219
PinAI ACCGGT 2 cut(s) 1697, 2252
PkrI GCNGC 8 cut(s) 53, 61, 162, 420, 922, 1040, 1113, 2683
PleI GAGTC 3 cut(s) 215, 742, 1114
PpsI GAGTC 3 cut(s) 215, 742, 1114
PpuMI RGGWCCY 1 cut(s) 1308
PsiI TTATAA 1 cut(s) 2615
Psp124BI GAGCTC 1 cut(s) 521
Psp5II RGGWCCY 1 cut(s) 1308
Psp6I CCWGG 9 cut(s) 24, 277, 628, 708, 822, 1457, 2019, 2212, 2518
PspEI GGTNACC 1 cut(s) 370
PspGI CCWGG 9 cut(s) 24, 277, 628, 708, 822, 1457, 2019, 2212, 2518
PspN4I GGNNCC 5 cut(s) 1309, 1422, 1759, 2181, 2692
PspPI GGNCC 5 cut(s) 145, 269, 786, 1308, 2623
PspPPI RGGWCCY 1 cut(s) 1308
PstI CTGCAG 3 cut(s) 1394, 1726, 2686
PsuI RGATCY 2 cut(s) 1665, 2369
PvuII CAGCTG 1 cut(s) 2681
RsaI GTAC 5 cut(s) 1971, 1993, 2041, 2576, 2665
RsaNI GTAC 5 cut(s) 1970, 1992, 2040, 2575, 2664
SacI GAGCTC 1 cut(s) 521
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 7 cut(s) 867, 1164, 1500, 1511, 2093, 2361, 2420
SatI GCNGC 8 cut(s) 52, 60, 161, 419, 921, 1039, 1112, 2682
Sau3AI GATC 9 cut(s) 244, 503, 880, 960, 1665, 1889, 2257, 2369, 2557
Sau96I GGNCC 5 cut(s) 145, 269, 786, 1308, 2623
ScaI AGTACT 2 cut(s) 2041, 2665
SchI GAGTC 3 cut(s) 215, 742, 1114
SduI GDGCHC 3 cut(s) 83, 521, 1861
SfaNI GCATC 5 cut(s) 615, 922, 950, 1627, 2505
SfcI CTRYAG 7 cut(s) 1278, 1390, 1722, 2407, 2655, 2676, 2682
SfuI TTCGAA 3 cut(s) 319, 2500, 2567
SinI GGWCC 4 cut(s) 145, 786, 1308, 2623
SmlI CTYRAG 7 cut(s) 152, 164, 611, 674, 1441, 1466, 2419
SmoI CTYRAG 7 cut(s) 152, 164, 611, 674, 1441, 1466, 2419
SsiI CCGC 8 cut(s) 52, 60, 105, 160, 182, 259, 419, 1597
SspI AATATT 3 cut(s) 910, 1435, 1480
SspMI CTAG 1 cut(s) 926
SstI GAGCTC 1 cut(s) 521
StyI CCWWGG 6 cut(s) 1092, 1333, 2434, 2619, 2626, 2694
TaaI ACNGT 2 cut(s) 468, 2083
TaiI ACGT 3 cut(s) 606, 2190, 2605
TaqI TCGA 8 cut(s) 42, 129, 319, 526, 2206, 2500, 2560, 2567
TatI WGTACW 2 cut(s) 2039, 2663
TauI GCSGC 4 cut(s) 54, 62, 163, 421
Tru1I TTAA 7 cut(s) 867, 1164, 1500, 1511, 2093, 2361, 2420
Tru9I TTAA 7 cut(s) 867, 1164, 1500, 1511, 2093, 2361, 2420
TscAI CASTG 6 cut(s) 184, 391, 471, 1236, 1599, 1833
TseFI GTSAC 4 cut(s) 370, 462, 2005, 2603
TseI GCWGC 4 cut(s) 920, 1038, 1111, 2681
Tsp45I GTSAC 4 cut(s) 370, 462, 2005, 2603
TspGWI ACGGA 3 cut(s) 237, 711, 2562
TspRI CASTG 6 cut(s) 184, 391, 471, 1236, 1599, 1833
Van91I CCANNNNNTGG 2 cut(s) 646, 2219
Vha464I CTTAAG 1 cut(s) 2419
VpaK11BI GGWCC 4 cut(s) 145, 786, 1308, 2623
XapI RAATTY 5 cut(s) 312, 966, 1013, 1618, 2493
XceI RCATGY 2 cut(s) 12, 2289
XmiI GTMKAC 1 cut(s) 1281
XmnI GAANNNNTTC 2 cut(s) 113, 2496
XspI CTAG 1 cut(s) 926
ZrmI AGTACT 2 cut(s) 2041, 2665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.