MD08G1160800.v1.1

NAD kinase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010084
Physical Location & Seq
Forward (+)
18628483 .. 18634603
6121 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1160800.v1.1.491

Sequence Viewer

Length: 3033 bp
ATGTGCCAGCTGGCCATCGTCGTCGTCGACATGAATCGGTGCGCTTCATCGCCTCCCCCTCTCTGCGTCTTTACGCCGTGCCAGTTCTTCGGCAGCAGCACCACCGCCGTCAAGTTCTTCAGGTTCGAGTTCGAGTTCCAGAGGAAAGAGCGATTCAGGCGGCGGCTTAAGTTCGTTGTCAGTGCAGAGCTTTCGAAGCCATTCGCTCTCAGCTTCGGCTTGGACTCTCAGACCTTTCGGCCTCATGATCCTTCGCAATTACCTAGAATTGGTCCTATTCCTGGAGATATTGCAGAAATTGAGGCATACTGTAGAATCTTTAGATGTGCTGAACGGCTTCATAGTGCATTGATGGACACATTATGCAATCCAGTAACGGGTGAATGTAGTGTTTATTATGATTACCCATCAGAGGAAAAACCACTTTTGGAGGATAAAATTGTCTCTGTGATTGGGTGTATGGTATCCCTGTTGAACAAAGGAAGGGAGGATGTGCTTTCTGGTAGATCGTCCATTCGCCTCGCAGATTTAAGTGTAATGGAGGATGAGCTTCCTCCACTCGCCATTTTCAGGAGTGAGGTGAAAAGGTGTTGCGAGAGCTTGCATGTTGCTCTTGAAAACTGGTTGGTTCCTGGGGATGATCGAAGCTTGGATGTATGGAGGAAACTTCAGAGACTGAAGAATGTCTGTTATGATTCTGGTTTTCCCCGCAGGGAGGATTATCCTTGCCATACACTTTTTGCGAATTGGGCTCCTGTTTACTTATCCTCTTCTAAAGAGGACATTAGGTCCGTAGATTCAGAGATAGCTTTTTGGAGGGGTGGCCAGGTAACAGAAGAAGGTCTGAAGTGGTTACTAGAGAAAGGATATAAAACTATTGTTGACCTTAGGGCAGAGACTGTAAAAGACAAAGCTTATCATTCAGCCATAGATGATTCTATTGCATCCGGGAAAGTTGAATTGGTCAAAATTCCAGTTGAAGTTGGGACTGCACCTTCAATGGAGCAGGTTGAGAAATTTGCATCTTTGGTTTCAGATTGCAGCAAGAAGCCGATCTATCTTCATAGTAAGGAAGGAGCGTTGAGAACTTCTGCCATGGTTTCTAGATGGAGGCAATACTCGACGCGTTATAATGTGCAGTTTGTCTCCAAGCGGTCAAGAGCTATCAACGATGTGTTACTACGAAATACAAATGGGGCAGGCGAAGTGCTGGAGCCTTCCACTTCTAAGAAAAGGTCTATCCCGGGAAAGAACAAGCCACTGCAAGGGGAACTGGACAAAACTTATGGTTTAAATGGCGTATTTCAAAAGGATGTGTCTCCAGATAGGGATGAAACAAATCAGAGCTCAGATGGGACATATAACAGCCTTATGTCTGTCCAAGGCATGACATCAGTAGAACCAGATGAAAATGGGGAAGGAAATATGATGAACTTTTGCAGGGAAGTTGAACCCCTGAATGCCCAAGTTCCTCCTTGTAATGTTTTCTCTAGAAAAGAAATGTCCAGGTTTCTGGGGAGAAAAAGCATCTCACCTCACTCCTATTTTAATCATCAGTTAAAAAGGTTGGCTACATTACCAATTTCCAGAGGTATCAATATTAAGACAATGCAGAGAGGCGGTACCAATTCGGCACCTCAGCTTGTGGTGGTACAAAATTCATATGGGCCACCTTACAGAAAGGATCTATCTCCAGAAGTCCAGACCTCTACTTCTGGAAATGGGAAGTATTTGACTAGTGTTAGTTCTGGTTCTGTTCTTCCAGTTGTGAACGGATTTGGTGAAGTGAATGAAATTGCAAGTAATGTGTCTACTACTCCGAGTAGTAGTTATGATGAGAGTGTCTTACCTAAAGCAGTAAATGAGGATAGGAAGAGTAATGGTGGAGCCACCTTAGCTTCAGGAGACGATGACCTGGGGTCAATTGAAGGAAATATGTGTGCTTCTGCAACTGGTGTTGTAAGGGTGCAGTCAAGAAAGAAAGCTGAGATGTTTTTAGTTCGAACAGATGGATTCTCTTGTAGCCGAGAAAAGGTGACGGAATCCTCCTTGGCCTTCACTCATCCAAGTACCCAGCAGCAAATGCTTATGTGGAAATCCTCACCAAAGACAGTATTAGTGTTGAAGAAGCTAGGGCAAGAACTCATGCAACAAGCTAAAGAGGTTGTCTCTTTCTTGTATTACCAAGAGAAAATGAATGTTCTGGTGGAACCAGATGTGCATGATGTGTTTGCTAGAATCCCAGGGTTTGGATTTGTTCAGACCTTCTATAGTCAAGATACCAGTGATCTGCATGAGAGGGTTGATTTTGTGGCATGCCTGGGAGGAGATGGAGTTATACTCCATGCTTCAAATCTGTTTAAAGGTGCTGTTCCCCCAATTGTATCATTTAATCTTGGATCTCTTGGATTTTTGACTTCCCATACGTTTGATGACTATATGCAGGACTTAAGGCAAGTGATCCATGGAAATAACACAAGTGATGGTGTTTATATAACTCTGAGAATGCGGCTCAGGTGTGAGATTTTTCGAAAGGGTAAAGCAATGCCTGGGAAAGTATTTGATGTCTTGAATGAAATAGTTGTTGATCGAGGTTCTAATCCATACCTTTCTAAGATTGAATGTTATGAACAAGACCGGCTTATAACCAAGGTCCAAGGTGATGGGGTCATTATTGCTACACCTACAGGAAGTACTGCATACTCTACAGCTGCAGGAGGTTCCATGGTGCATCCAAATGTCCCGTGCATGTTGTTTACCCCAATCTGCCCACACTCCCTCTCATTTAGACCAGTTATACTTCCAGATTCTGCAAAGCTTGAATTGAAGATTCCAAGCGATGCTCGAAGCAATGCCTGGGTTTCTTTTGACGGAAAGAGAAGGCAGCAACTCTCAAGAGGAGACTCTGTCCGAATATCTATGAGCCAACACCCACTTCCAACTGTTAACAAACGTGACCAAACAGGTGATTGGTTTCGAAGCTTGATTCGTTGCCTAAACTGGAACGAAAGACTAGATCAAAAGGCCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

1011

Amino Acids

112.62

Weight (kDa)

8.34

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-NADK PF22741 242 - 380 1.6e-63 DSP-PTPase phosphatase fused to NAD+ Kinase
Tc-R-P PF22785 276 - 370 3.2e-10 Polymorphic toxin system, DSP-PTPase phosphatase
NAD_kinase PF01513 711 - 823 5.4e-15 ATP-NAD kinase N-terminal domain
NAD_kinase_C PF20143 857 - 983 1.4e-35 ATP-NAD kinase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1131, 2645
Acc36I ACCTGC 1 cut(s) 997
Acc65I GGTACC 1 cut(s) 1622
AccB1I GGYRCC 2 cut(s) 1622, 1633
AccB7I CCANNNNNTGG 1 cut(s) 2249
AccI GTMKAC 2 cut(s) 27, 1811
AccII CGCG 1 cut(s) 1126
AciI CCGC 7 cut(s) 105, 160, 163, 709, 1153, 1620, 2509
AclWI GGATC 4 cut(s) 242, 1692, 2407, 2455
AcoI YGGCCR 2 cut(s) 12, 823
AcsI RAATTY 3 cut(s) 969, 1016, 1657
AcuI CTGAAG 5 cut(s) 103, 653, 698, 866, 1884
AfaI GTAC 4 cut(s) 1624, 1653, 2071, 2695
AfiI CCNNNNNNNGG 9 cut(s) 269, 281, 377, 412, 570, 715, 1265, 2032, 2249
AflII CTTAAG 2 cut(s) 167, 2449
AflIII ACRYGT 1 cut(s) 1124
AhdI GACNNNNNGTC 2 cut(s) 787, 1918
AhlI ACTAGT 1 cut(s) 1736
AjnI CCWGG 9 cut(s) 280, 631, 825, 1505, 1914, 2242, 2319, 2548, 2855
AjuI GAANNNNNNNTTGG 4 cut(s) 944, 976, 2919, 2951
Alw21I GWGCWC 1 cut(s) 1349
Alw26I GTCTC 8 cut(s) 448, 667, 890, 1150, 1323, 1899, 2173, 2895
AlwI GGATC 4 cut(s) 242, 1692, 2407, 2455
AlwNI CAGNNNCTG 3 cut(s) 676, 899, 2810
Ama87I CYCGRG 1 cut(s) 1243
AoxI GGCC 6 cut(s) 12, 239, 823, 1667, 2052, 3024
ApeKI GCWGC 6 cut(s) 93, 96, 1041, 2077, 2711, 2884
ApoI RAATTY 3 cut(s) 969, 1016, 1657
Asp700I GAANNNNTTC 2 cut(s) 200, 336
Asp718I GGTACC 1 cut(s) 1622
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 5 cut(s) 272, 789, 1667, 2653, 3025
AsuC2I CCSGG 3 cut(s) 949, 1244, 1245
AsuHPI GGTGA 8 cut(s) 392, 592, 1524, 1793, 2047, 2094, 2672, 2978
AsuII TTCGAA 4 cut(s) 194, 2002, 2530, 2977
AvaI CYCGRG 1 cut(s) 1243
AvaII GGWCC 3 cut(s) 272, 789, 2653
AxyI CCTNAGG 1 cut(s) 887
BalI TGGCCA 2 cut(s) 14, 825
BanI GGYRCC 2 cut(s) 1622, 1633
BanII GRGCYC 2 cut(s) 754, 1349
Bbv12I GWGCWC 1 cut(s) 1349
BbvCI CCTCAGC 1 cut(s) 1638
BbvI GCAGC 6 cut(s) 105, 108, 1053, 2089, 2698, 2896
BccI CCATC 9 cut(s) 23, 346, 415, 1101, 1346, 2003, 2324, 2477, 2657
BceAI ACGGC 3 cut(s) 61, 92, 350
BcgI CGANNNNNNTGC 2 cut(s) 174, 208
BciT130I CCWGG 9 cut(s) 282, 633, 827, 1507, 1916, 2244, 2321, 2550, 2857
BciVI GTATCC 1 cut(s) 475
BcnI CCSGG 3 cut(s) 949, 1244, 1245
BcoDI GTCTC 8 cut(s) 448, 667, 890, 1150, 1323, 1899, 2173, 2895
BcuI ACTAGT 1 cut(s) 1736
BfaI CTAG 8 cut(s) 264, 857, 1104, 1491, 1737, 2132, 2235, 3014
BfmI CTRYAG 5 cut(s) 310, 2269, 2685, 2706, 2712
BfrI CTTAAG 2 cut(s) 167, 2449
BfuAI ACCTGC 1 cut(s) 997
BfuI GTATCC 1 cut(s) 475
BisI GCNGC 9 cut(s) 94, 97, 161, 164, 1042, 2078, 2510, 2712, 2885
BlsI GCNGC 9 cut(s) 95, 98, 162, 165, 1043, 2079, 2511, 2713, 2886
BmcAI AGTACT 1 cut(s) 2695
Bme18I GGWCC 3 cut(s) 272, 789, 2653
BmeRI GACNNNNNGTC 2 cut(s) 787, 1918
BmeT110I CYCGRG 1 cut(s) 1243
BmgT120I GGNCC 5 cut(s) 272, 789, 1667, 2653, 3025
BmiI GGNNCC 8 cut(s) 630, 753, 1215, 1624, 1635, 1888, 2211, 2722
BmsI GCATC 5 cut(s) 953, 1031, 1536, 2740, 2830
BplI GAGNNNNNCTC 4 cut(s) 2153, 2185, 2325, 2357
BpmI CTGGAG 4 cut(s) 303, 1232, 1305, 1677
Bpu10I CCTNAGC 3 cut(s) 1638, 1894, 2513
Bpu14I TTCGAA 4 cut(s) 194, 2002, 2530, 2977
BpuEI CTTGAG 1 cut(s) 2878
BpuMI CCSGG 3 cut(s) 949, 1244, 1245
BsaXI ACNNNNNCTCC 4 cut(s) 2319, 2349, 2892, 2922
Bsc4I CCNNNNNNNGG 9 cut(s) 269, 281, 377, 412, 570, 715, 1265, 2032, 2249
Bse118I RCCGGY 1 cut(s) 2637
Bse21I CCTNAGG 1 cut(s) 887
Bse3DI GCAATG 2 cut(s) 2550, 2857
BseBI CCWGG 9 cut(s) 282, 633, 827, 1507, 1916, 2244, 2321, 2550, 2857
BseGI GGATG 9 cut(s) 496, 550, 643, 658, 944, 1318, 1336, 2062, 2731
BseLI CCNNNNNNNGG 9 cut(s) 269, 281, 377, 412, 570, 715, 1265, 2032, 2249
BseMI GCAATG 2 cut(s) 2550, 2857
BseMII CTCAG 7 cut(s) 223, 242, 1362, 1652, 1977, 2492, 2527
BseRI GAGGAG 2 cut(s) 2340, 2913
BseXI GCAGC 6 cut(s) 105, 108, 1053, 2089, 2698, 2896
BseYI CCCAGC 1 cut(s) 2073
BsgI GTGCAG 4 cut(s) 204, 975, 1157, 1988
Bsh1236I CGCG 1 cut(s) 1126
BshFI GGCC 6 cut(s) 14, 241, 825, 1669, 2054, 3026
BshNI GGYRCC 2 cut(s) 1622, 1633
BsiHKAI GWGCWC 1 cut(s) 1349
BsiHKCI CYCGRG 1 cut(s) 1243
BsiSI CCGG 3 cut(s) 948, 1244, 2638
BslFI GGGAC 3 cut(s) 1000, 1369, 2726
BslI CCNNNNNNNGG 9 cut(s) 269, 281, 377, 412, 570, 715, 1265, 2032, 2249
BsmAI GTCTC 8 cut(s) 448, 667, 890, 1150, 1323, 1899, 2173, 2895
BsmBI CGTCTC 1 cut(s) 1899
BsmFI GGGAC 3 cut(s) 1000, 1369, 2726
BsmI GAATGC 2 cut(s) 1465, 2511
BsnI GGCC 6 cut(s) 14, 241, 825, 1669, 2054, 3026
BsoBI CYCGRG 1 cut(s) 1243
Bsp119I TTCGAA 4 cut(s) 194, 2002, 2530, 2977
Bsp1286I GDGCHC 2 cut(s) 754, 1349
Bsp19I CCATGG 3 cut(s) 1095, 2464, 2724
BspACI CCGC 7 cut(s) 105, 160, 163, 709, 1153, 1620, 2509
BspANI GGCC 6 cut(s) 14, 241, 825, 1669, 2054, 3026
BspCNI CTCAG 7 cut(s) 222, 241, 1361, 1651, 1978, 2493, 2526
BspFNI CGCG 1 cut(s) 1126
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 8 cut(s) 630, 753, 1215, 1624, 1635, 1888, 2211, 2722
BspMAI CTGCAG 1 cut(s) 2716
BspMI ACCTGC 1 cut(s) 997
BspPI GGATC 4 cut(s) 242, 1692, 2407, 2455
BspT104I TTCGAA 4 cut(s) 194, 2002, 2530, 2977
BspT107I GGYRCC 2 cut(s) 1622, 1633
BspTI CTTAAG 2 cut(s) 167, 2449
BsrDI GCAATG 2 cut(s) 2550, 2857
BsrFI RCCGGY 1 cut(s) 2637
BssAI RCCGGY 1 cut(s) 2637
BssT1I CCWWGG 7 cut(s) 1095, 1381, 2049, 2464, 2649, 2656, 2724
Bst2UI CCWGG 9 cut(s) 282, 633, 827, 1507, 1916, 2244, 2321, 2550, 2857
Bst4CI ACNGT 4 cut(s) 311, 901, 2113, 2944
Bst6I CTCTTC 2 cut(s) 775, 1868
BstAFI CTTAAG 2 cut(s) 167, 2449
BstAPI GCANNNNNTGC 1 cut(s) 2083
BstBI TTCGAA 4 cut(s) 194, 2002, 2530, 2977
BstC8I GCNNGC 5 cut(s) 8, 12, 602, 1201, 2317
BstDSI CCRYGG 3 cut(s) 1095, 2464, 2724
BstF5I GGATG 9 cut(s) 496, 550, 643, 658, 944, 1318, 1336, 2062, 2731
BstFNI CGCG 1 cut(s) 1126
BstHHI GCGC 1 cut(s) 44
BstMAI GTCTC 8 cut(s) 448, 667, 890, 1150, 1323, 1899, 2173, 2895
BstMWI GCNNNNNNNGC 5 cut(s) 157, 196, 749, 1895, 2083
BstNI CCWGG 9 cut(s) 282, 633, 827, 1507, 1916, 2244, 2321, 2550, 2857
BstNSI RCATGY 3 cut(s) 608, 2319, 2752
BstSFI CTRYAG 5 cut(s) 310, 2269, 2685, 2706, 2712
BstUI CGCG 1 cut(s) 1126
BstV1I GCAGC 6 cut(s) 105, 108, 1053, 2089, 2698, 2896
BstX2I RGATCY 2 cut(s) 1684, 2399
BstXI CCANNNNNNTGG 2 cut(s) 1513, 2663
BstYI RGATCY 2 cut(s) 1684, 2399
Bsu36I CCTNAGG 1 cut(s) 887
BsuI GTATCC 1 cut(s) 475
BsuRI GGCC 6 cut(s) 14, 241, 825, 1669, 2054, 3026
BtgI CCRYGG 3 cut(s) 1095, 2464, 2724
BtgZI GCGATG 2 cut(s) 33, 2853
BtsCI GGATG 9 cut(s) 496, 550, 643, 658, 944, 1318, 1336, 2062, 2731
BtsI GCAGTG 1 cut(s) 1259
BtsIMutI CAGTG 3 cut(s) 187, 1259, 2290
BveI ACCTGC 1 cut(s) 997
Cac8I GCNNGC 5 cut(s) 8, 12, 602, 1201, 2317
CaiI CAGNNNCTG 3 cut(s) 676, 899, 2810
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 44
Cfr10I RCCGGY 1 cut(s) 2637
Cfr13I GGNCC 5 cut(s) 272, 789, 1667, 2653, 3025
Cfr9I CCCGGG 1 cut(s) 1243
CseI GACGC 2 cut(s) 55, 1132
Csp6I GTAC 4 cut(s) 1623, 1652, 2070, 2694
CviQI GTAC 4 cut(s) 1623, 1652, 2070, 2694
DraI TTTAAA 2 cut(s) 1293, 2362
DriI GACNNNNNGTC 2 cut(s) 787, 1918
EaeI YGGCCR 2 cut(s) 12, 823
Eam1104I CTCTTC 2 cut(s) 775, 1868
Eam1105I GACNNNNNGTC 2 cut(s) 787, 1918
EarI CTCTTC 2 cut(s) 775, 1868
Ecl136II GAGCTC 1 cut(s) 1347
Eco130I CCWWGG 7 cut(s) 1095, 1381, 2049, 2464, 2649, 2656, 2724
Eco24I GRGCYC 2 cut(s) 754, 1349
Eco47I GGWCC 3 cut(s) 272, 789, 2653
Eco53kI GAGCTC 1 cut(s) 1347
Eco57I CTGAAG 5 cut(s) 103, 653, 698, 866, 1884
Eco81I CCTNAGG 1 cut(s) 887
Eco88I CYCGRG 1 cut(s) 1243
EcoICRI GAGCTC 1 cut(s) 1347
EcoO109I RGGNCCY 1 cut(s) 3025
EcoRII CCWGG 9 cut(s) 280, 631, 825, 1505, 1914, 2242, 2319, 2548, 2855
EcoT14I CCWWGG 7 cut(s) 1095, 1381, 2049, 2464, 2649, 2656, 2724
EcoT38I GRGCYC 2 cut(s) 754, 1349
ErhI CCWWGG 7 cut(s) 1095, 1381, 2049, 2464, 2649, 2656, 2724
Esp3I CGTCTC 1 cut(s) 1899
FalI AAGNNNNNCTT 2 cut(s) 2625, 2657
FaqI GGGAC 3 cut(s) 1000, 1369, 2726
FauI CCCGC 1 cut(s) 716
FauNDI CATATG 1 cut(s) 1663
FblI GTMKAC 2 cut(s) 27, 1811
Fnu4HI GCNGC 9 cut(s) 94, 97, 161, 164, 1042, 2078, 2510, 2712, 2885
FokI GGATG 9 cut(s) 503, 557, 650, 665, 931, 1325, 1343, 2049, 2718
FriOI GRGCYC 2 cut(s) 754, 1349
Fsp4HI GCNGC 9 cut(s) 94, 97, 161, 164, 1042, 2078, 2510, 2712, 2885
FspBI CTAG 8 cut(s) 264, 857, 1104, 1491, 1737, 2132, 2235, 3014
GlaI GCGC 1 cut(s) 43
GluI GCNGC 9 cut(s) 94, 97, 161, 164, 1042, 2078, 2510, 2712, 2885
GsaI CCCAGC 1 cut(s) 2077
GsuI CTGGAG 4 cut(s) 303, 1232, 1305, 1677
HaeIII GGCC 6 cut(s) 14, 241, 825, 1669, 2054, 3026
HapII CCGG 3 cut(s) 948, 1244, 2638
HgaI GACGC 2 cut(s) 55, 1132
HhaI GCGC 1 cut(s) 44
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 3 cut(s) 28, 883, 2947
HindII GTYRAC 3 cut(s) 28, 883, 2947
HindIII AAGCTT 4 cut(s) 646, 912, 2816, 2980
HpaI GTTAAC 1 cut(s) 2947
HpaII CCGG 3 cut(s) 948, 1244, 2638
HphI GGTGA 8 cut(s) 392, 592, 1524, 1793, 2047, 2094, 2672, 2978
Hpy166II GTNNAC 7 cut(s) 28, 760, 883, 1771, 1812, 2757, 2947
Hpy8I GTNNAC 7 cut(s) 28, 760, 883, 1771, 1812, 2757, 2947
Hpy99I CGWCG 4 cut(s) 23, 26, 29, 1126
HpyCH4III ACNGT 4 cut(s) 311, 901, 2113, 2944
HpyCH4IV ACGT 2 cut(s) 2426, 2953
HpyF10VI GCNNNNNNNGC 5 cut(s) 157, 196, 749, 1895, 2083
HpySE526I ACGT 2 cut(s) 2426, 2953
HspAI GCGC 1 cut(s) 42
KpnI GGTACC 1 cut(s) 1626
KspAI GTTAAC 1 cut(s) 2947
LmnI GCTCC 5 cut(s) 757, 1003, 1076, 1213, 1886
Lsp1109I GCAGC 6 cut(s) 105, 108, 1053, 2089, 2698, 2896
LweI GCATC 5 cut(s) 953, 1031, 1536, 2740, 2830
MaeI CTAG 8 cut(s) 264, 857, 1104, 1491, 1737, 2132, 2235, 3014
MaeII ACGT 2 cut(s) 2426, 2953
MaeIII GTNAC 6 cut(s) 373, 829, 852, 1176, 2035, 2954
MfeI CAATTG 2 cut(s) 1923, 2379
MflI RGATCY 2 cut(s) 1684, 2399
MhlI GDGCHC 2 cut(s) 754, 1349
MlsI TGGCCA 2 cut(s) 14, 825
MluI ACGCGT 1 cut(s) 1124
MluNI TGGCCA 2 cut(s) 14, 825
MlyI GAGTC 2 cut(s) 218, 2897
MmeI TCCRAC 1 cut(s) 2963
Mox20I TGGCCA 2 cut(s) 14, 825
MroXI GAANNNNTTC 2 cut(s) 200, 336
MscI TGGCCA 2 cut(s) 14, 825
MslI CAYNNNNRTG 1 cut(s) 2736
Msp20I TGGCCA 2 cut(s) 14, 825
MspA1I CMGCKG 2 cut(s) 10, 2711
MspCI CTTAAG 2 cut(s) 167, 2449
MspI CCGG 3 cut(s) 948, 1244, 2638
MunI CAATTG 2 cut(s) 1923, 2379
Mva1269I GAATGC 2 cut(s) 1465, 2511
MvaI CCWGG 9 cut(s) 282, 633, 827, 1507, 1916, 2244, 2321, 2550, 2857
MvnI CGCG 1 cut(s) 1126
MwoI GCNNNNNNNGC 5 cut(s) 157, 196, 749, 1895, 2083
NciI CCSGG 3 cut(s) 949, 1244, 1245
NcoI CCATGG 3 cut(s) 1095, 2464, 2724
NdeI CATATG 1 cut(s) 1663
NlaIV GGNNCC 8 cut(s) 630, 753, 1215, 1624, 1635, 1888, 2211, 2722
NmeAIII GCCGAG 1 cut(s) 2051
NmuCI GTSAC 2 cut(s) 2035, 2954
NspI RCATGY 3 cut(s) 608, 2319, 2752
NspV TTCGAA 4 cut(s) 194, 2002, 2530, 2977
PaeI GCATGC 1 cut(s) 2319
PagI TCATGA 1 cut(s) 244
PasI CCCWGGG 1 cut(s) 2243
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PctI GAATGC 2 cut(s) 1465, 2511
PdmI GAANNNNTTC 2 cut(s) 200, 336
PflFI GACNNNGTC 1 cut(s) 2906
PflMI CCANNNNNTGG 1 cut(s) 2249
PfoI TCCNGGA 2 cut(s) 280, 947
PkrI GCNGC 9 cut(s) 95, 98, 162, 165, 1043, 2079, 2511, 2713, 2886
PleI GAGTC 2 cut(s) 218, 2897
PpsI GAGTC 2 cut(s) 218, 2897
PsiI TTATAA 2 cut(s) 1131, 2645
Psp124BI GAGCTC 1 cut(s) 1349
Psp6I CCWGG 9 cut(s) 280, 631, 825, 1505, 1914, 2242, 2319, 2548, 2855
PspFI CCCAGC 1 cut(s) 2073
PspGI CCWGG 9 cut(s) 280, 631, 825, 1505, 1914, 2242, 2319, 2548, 2855
PspN4I GGNNCC 8 cut(s) 630, 753, 1215, 1624, 1635, 1888, 2211, 2722
PspPI GGNCC 5 cut(s) 272, 789, 1667, 2653, 3025
PstI CTGCAG 1 cut(s) 2716
PstNI CAGNNNCTG 3 cut(s) 676, 899, 2810
PsuI RGATCY 2 cut(s) 1684, 2399
PsyI GACNNNGTC 1 cut(s) 2906
PvuII CAGCTG 2 cut(s) 10, 2711
RsaI GTAC 4 cut(s) 1624, 1653, 2071, 2695
RsaNI GTAC 4 cut(s) 1623, 1652, 2070, 2694
RseI CAYNNNNRTG 1 cut(s) 2736
SacI GAGCTC 1 cut(s) 1349
SalI GTCGAC 1 cut(s) 26
SatI GCNGC 9 cut(s) 94, 97, 161, 164, 1042, 2078, 2510, 2712, 2885
Sau96I GGNCC 5 cut(s) 272, 789, 1667, 2653, 3025
ScaI AGTACT 1 cut(s) 2695
SchI GAGTC 2 cut(s) 218, 2897
SduI GDGCHC 2 cut(s) 754, 1349
SfaNI GCATC 5 cut(s) 953, 1031, 1536, 2740, 2830
SfcI CTRYAG 5 cut(s) 310, 2269, 2685, 2706, 2712
SfuI TTCGAA 4 cut(s) 194, 2002, 2530, 2977
SinI GGWCC 3 cut(s) 272, 789, 2653
SmaI CCCGGG 1 cut(s) 1245
SmiMI CAYNNNNRTG 1 cut(s) 2736
SmlI CTYRAG 3 cut(s) 167, 2449, 2893
SmoI CTYRAG 3 cut(s) 167, 2449, 2893
SpeI ACTAGT 1 cut(s) 1736
SphI GCATGC 1 cut(s) 2319
SsiI CCGC 7 cut(s) 105, 160, 163, 709, 1153, 1620, 2509
SspI AATATT 1 cut(s) 1600
SspMI CTAG 8 cut(s) 264, 857, 1104, 1491, 1737, 2132, 2235, 3014
SstI GAGCTC 1 cut(s) 1349
StyI CCWWGG 7 cut(s) 1095, 1381, 2049, 2464, 2649, 2656, 2724
TaaI ACNGT 4 cut(s) 311, 901, 2113, 2944
TaiI ACGT 2 cut(s) 2429, 2956
TatI WGTACW 1 cut(s) 2693
TauI GCSGC 3 cut(s) 163, 166, 2512
TscAI CASTG 3 cut(s) 187, 1266, 2290
TseFI GTSAC 2 cut(s) 2035, 2954
TseI GCWGC 6 cut(s) 93, 96, 1041, 2077, 2711, 2884
Tsp45I GTSAC 2 cut(s) 2035, 2954
TspGWI ACGGA 4 cut(s) 781, 1788, 2054, 2886
TspMI CCCGGG 1 cut(s) 1243
TspRI CASTG 3 cut(s) 187, 1266, 2290
Tth111I GACNNNGTC 1 cut(s) 2906
Van91I CCANNNNNTGG 1 cut(s) 2249
Vha464I CTTAAG 2 cut(s) 167, 2449
VpaK11BI GGWCC 3 cut(s) 272, 789, 2653
XapI RAATTY 3 cut(s) 969, 1016, 1657
XbaI TCTAGA 2 cut(s) 1103, 1490
XceI RCATGY 3 cut(s) 608, 2319, 2752
XmaI CCCGGG 1 cut(s) 1243
XmiI GTMKAC 2 cut(s) 27, 1811
XmnI GAANNNNTTC 2 cut(s) 200, 336
XspI CTAG 8 cut(s) 264, 857, 1104, 1491, 1737, 2132, 2235, 3014
ZrmI AGTACT 1 cut(s) 2695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.