RLG00000011671

NAD kinase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010084
Physical Location & Seq
Forward (+)
11562521 .. 11567086
4566 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011671

Sequence Viewer

Length: 3003 bp
ATGTGCCAGGTGGCCATCGCCGTCGATATGAGCCGCTGTGCCGCTTCACCTCCGAATCTGTGCCCCCTCCAGTTCTCAGGTAGCCGCGCCAAGCTATTCGGATTGGGGTTGGACATTCAGAAGAAGGACCGGCTGAAGAGGCGGCTCAAGTTCGTCGTCAGTGCGGAGCTGTCCAAGCCATTCTCTGTGAGTTTCGGCTTGGACTCTCAGGGCTATCAGCTTCTTGATCCGTCGCAGTCGCGGAGGATTGGCCCTGTTCCTGGGGATATTGCAGAAATTGAAGCCTATTGTAGAATTTTTCGAAGTGCTGAACGACTACATACGGCATTGATGGAGACATTGTGCAATCCGGTGACCGGAGTATGTAGTGTTTATTATGACTTCCCATCGGAGGAAAAGCCGCTCTTGGAGGATAAGATTGTGTCTGTGATTGGTTGCATGATGTCACTGTTGAACAAAGCAAGGGAGGATGTGCTTTCTGGAAGATCAGCCCTTATGAGCTCCTTTCGAGGAGTTGTGGATGTCGGTGAGGTGGTGGAGGACATGCTTCCTCCACTTGCTGTTTTCAGGAGTGAGCTCAAGAGGTGTTGCGAGAGCTTGCACGTTGCGCTTGAGGACTGGTTGATGCCTGGGGATGACCAGAGCTTGGATGTGTGGAGGAAACTTCAGAGGCTGAAGAATGTGTGTTATGATTCCGGTTTTCCACGCCAGGAGGATTATCCGTGCAACACGCTTTTCGCTAATTGGACTCCTGTTTACTTATCGTCTTCTAAAGAGGATGTTGGCTCCGAAGATTCAGAGGTAGCGTTTTGGAGGGGTGGCCAGGTAACAGAAGAAGGTCTGGAGTGGTTACTGGAGAGAGGATTTAAAACTATTGTTGATCTTAGGGCAGAGATTGTAAAAGACAATATTTATCAGGCAGCCGTCGATGATGCTATTGCATCAGGAAAGGTGGAATTGATCAGAATTCCAGTTGAAGTTGGGACTGCACCCTCAAAGGAGCAGGTTGAGAAATTCGCAAGTTTGGTTTCAGATTGCAGCAAAAAGCCAATCTATCTTCATAGCAAGGAAGGTCTGTGGAGAACATCTGCCATGGTTTCCAGATGGAGGCAGCACTTGACTCGCCGTGCTGTTCAGTTTGTCTCCAAGCAGTCCGTTTCTCTTCATGGAGAAGTTGAAAAACCCTCAACTATGCAGAAAAGTTCCCTCCTTGAAAAGAAGAATGAGTCACTGGACACAATTCATGGTTCAAATGGGGTGCCAGAAAAAAATGATTCTGTAGACAGGAATGAAGCAAATCTAAATGGGACCTGCAATGGCCTTATATCTATCCAAGGTTTGACATCAGTTGAATCAGATGAAAGTGGGGAAAAACCTCTGGTGAACTTCTGCAGGGAAGTTGACCCCCTGAATGCTCAGGTTCCTCCTTGCAATATTTTCTCAAGAAAAGAAATGTCCAGGTTTCTTGAGAGAAAAAATATTGCACCACTCACTTATTTTAATTTTCAGTTAAATAGGTTGAGGGTATTACCAATTTCCAGATATATGAATACTAAGATAATGTGGAGAGGTGAGATTGTTGATACTGATTCAGTGCGGGAGGTTGTGGAGGCAGAAAATTCCAATGGGATACCTGATGCAGAACATCTGCTTCCAGAGTTCCAGATCTCTGCTTCTGGCAATGGGAATTATTTGACCGGTGTTGGTAATGGTTCTGTAACTGCAGTCGTAAATGGATTTGGTGAAGGGGAGAAAGGTTCCTTGTTAGCCACTAATTTATCTACTAACGCGAGCAACAATCATTCTGAGAGTGTCTTATCTAAAGCAGTGAAGGAGGAAAGGAAGAGTAATGGTAGAGCCCCTTTAGTTTCAAGTGATGATGAATTGGGATCAATTGAAGGAGATATGTGTGCTTCCGCAACTGGGGTTGTAAGGGTGCAGTCAAGAAAGAAAGCAGAGATGTTTTTAGTACGAACGGATGGATTCTCTTGTACCCGAGAAAAAGTGACAGAATCCTCCCTGGCCTTCACTCATCCAAGTACCCAGCAACAAATGCTTATGTGGAAATCTACTCCAAAGACAGTATTAGTGTTAAAGAAGCTTGGGCATGAGCTTATGGAACAAGCTAAAGAGGTTGTCTCCTTCTTGTATTACCAAGAGAAAATGAATGTTCTGGTGGAACCTGACGTGCATGATGTATTTGCTAGAATCCCAGGTTTTGGATTTGTTCAGACCTTTTACACCCAAGATACCGGTGATCTGCATGAGAGGGTTGATTTTGTGGCATGTCTGGGAGGGGATGGGGTAATACTCCATGCTTCAAATCTATTCAAAGGTGCTGTTCCCCCCATCGTATCATTTAATCTTGGATCTCTTGGATTTTTGACTTCCCATACTTTTGATGACTACAGGCAGGACTTAAGACAGGTTATCCATGGAAATAACACAAGTGATGGTGTTTATATAACTCTCAGAATGCGTCTCAGGTGTGAAATTTTTCGAAATGGTAAAGCGATGCCTGGAAAGGTATTTGATGTTTTGAATGAAATAGTTGTTGATCGAGGTTCGAATCCGTACCTTTCTAAGATTGAATGTTACGAACGTGACCAGCTTATAACCAAGGTCCAAGGTGACGGTGTCATTGTAGCCACACCTACAGGAAGTACTGCCTATTCTACAGCTGCAGGAGGTTCCATGGTGCATCCAAATGTTCCTTGCATGTTGTTTACGCCAATCTGCCCACACTCCCTCTCATTTAGACCAGTCATACTTCCAGATTCTGCAAGCCTTGAATTAAAGATTCCAGAGGATGCTCGAAGCAATGCCTGGGTTTCTTTTGATGGGAAGAGAAGGCAGCAGCTCTCAAGGGGAGACTCTGTTCGAATAGCTATGAGCCAACACCCACTTCCAACTGTCAACAAGCGTGACCAAACAGGGGACTGGTTTCGAAGCTTGATTCGTTGCCTAAATTGGAATGAGAGGCTAGATCAAAAGGCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

1001

Amino Acids

111.2

Weight (kDa)

6.08

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-NADK PF22741 241 - 384 2.5e-64 DSP-PTPase phosphatase fused to NAD+ Kinase
Tc-R-P PF22785 275 - 369 7.6e-11 Polymorphic toxin system, DSP-PTPase phosphatase
NAD_kinase PF01513 703 - 813 4.7e-15 ATP-NAD kinase N-terminal domain
NAD_kinase_C PF20143 847 - 973 6.5e-35 ATP-NAD kinase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2615
Acc36I ACCTGC 2 cut(s) 994, 1319
AccB1I GGYRCC 1 cut(s) 1258
AccB7I CCANNNNNTGG 2 cut(s) 646, 2219
AccBSI CCGCTC 1 cut(s) 403
AccI GTMKAC 1 cut(s) 1281
AccII CGCG 3 cut(s) 87, 241, 1790
AciI CCGC 9 cut(s) 34, 42, 85, 142, 164, 241, 401, 1597, 1917
AclWI GGATC 3 cut(s) 221, 1897, 2377
AcoI YGGCCR 2 cut(s) 12, 820
AcsI RAATTY 5 cut(s) 294, 966, 1013, 1618, 2493
AcuI CTGAAG 3 cut(s) 155, 650, 695
AfaI GTAC 5 cut(s) 1971, 1993, 2041, 2576, 2665
AfiI CCNNNNNNNGG 8 cut(s) 260, 356, 391, 646, 1107, 1923, 2219, 2935
AflII CTTAAG 1 cut(s) 2419
AgeI ACCGGT 2 cut(s) 1697, 2252
AjiI CACGTC 1 cut(s) 2188
AjuI GAANNNNNNNTTGG 2 cut(s) 2889, 2921
AloI GAACNNNNNNTCC 2 cut(s) 2862, 2894
Alw21I GWGCWC 2 cut(s) 503, 579
Alw26I GTCTC 5 cut(s) 329, 1147, 2143, 2486, 2865
AlwI GGATC 3 cut(s) 221, 1897, 2377
AlwNI CAGNNNCTG 2 cut(s) 673, 2780
Ama87I CYCGRG 1 cut(s) 1995
AoxI GGCC 6 cut(s) 12, 250, 820, 1318, 2022, 2994
ApeKI GCWGC 6 cut(s) 920, 1038, 1111, 2681, 2854, 2857
ApoI RAATTY 5 cut(s) 294, 966, 1013, 1618, 2493
AsiGI ACCGGT 2 cut(s) 1697, 2252
Asp700I GAANNNNTTC 1 cut(s) 2496
AspLEI GCGC 2 cut(s) 89, 610
AspS9I GGNCC 5 cut(s) 127, 251, 1308, 2623, 2995
AsuHPI GGTGA 8 cut(s) 39, 364, 539, 1393, 1583, 1754, 2267, 2642
AsuII TTCGAA 5 cut(s) 301, 2500, 2567, 2881, 2947
AvaI CYCGRG 1 cut(s) 1995
AvaII GGWCC 3 cut(s) 127, 1308, 2623
BaeGI GKGCMC 1 cut(s) 65
BalI TGGCCA 2 cut(s) 14, 822
BanI GGYRCC 1 cut(s) 1258
BanII GRGCYC 3 cut(s) 503, 579, 1861
BbsI GAAGAC 1 cut(s) 759
Bbv12I GWGCWC 2 cut(s) 503, 579
BbvI GCAGC 6 cut(s) 932, 1050, 1123, 2668, 2866, 2869
BccI CCATC 9 cut(s) 23, 325, 394, 1098, 1973, 2294, 2357, 2447, 2834
BceAI ACGGC 4 cut(s) 5, 339, 908, 1110
BcgI CGANNNNNNTGC 2 cut(s) 1103, 1137
BciVI GTATCC 1 cut(s) 1623
BclI TGATCA 1 cut(s) 960
BcoDI GTCTC 5 cut(s) 329, 1147, 2143, 2486, 2865
BfaI CTAG 2 cut(s) 2205, 2984
BfmI CTRYAG 7 cut(s) 1278, 1390, 1722, 2407, 2655, 2676, 2682
BfrI CTTAAG 1 cut(s) 2419
BfuAI ACCTGC 2 cut(s) 994, 1319
BfuI GTATCC 1 cut(s) 1623
BglII AGATCT 1 cut(s) 1665
BmcAI AGTACT 1 cut(s) 2665
Bme18I GGWCC 3 cut(s) 127, 1308, 2623
BmeT110I CYCGRG 1 cut(s) 1995
BmgBI CACGTC 1 cut(s) 2188
BmgT120I GGNCC 5 cut(s) 127, 251, 1308, 2623, 2995
BmiI GGNNCC 7 cut(s) 787, 1260, 1309, 1422, 1759, 2181, 2692
BmrI ACTGGG 1 cut(s) 1932
BmsI GCATC 7 cut(s) 615, 922, 950, 1627, 2505, 2710, 2800
BmuI ACTGGG 1 cut(s) 1932
BpiI GAAGAC 1 cut(s) 759
BplI GAGNNNNNCTC 2 cut(s) 2123, 2155
BpmI CTGGAG 3 cut(s) 53, 863, 875
Bpu10I CCTNAGC 1 cut(s) 1416
Bpu14I TTCGAA 5 cut(s) 301, 2500, 2567, 2881, 2947
BpuEI CTTGAG 6 cut(s) 131, 563, 632, 1426, 1487, 2848
BsaWI WCCGGW 5 cut(s) 349, 356, 695, 1697, 2252
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 2862, 2892
Bsc4I CCNNNNNNNGG 8 cut(s) 260, 356, 391, 646, 1107, 1923, 2219, 2935
Bse118I RCCGGY 3 cut(s) 129, 1697, 2252
Bse1I ACTGG 8 cut(s) 70, 623, 858, 971, 1236, 1927, 2762, 2945
Bse3DI GCAATG 3 cut(s) 1321, 1687, 2827
BseLI CCNNNNNNNGG 8 cut(s) 260, 356, 391, 646, 1107, 1923, 2219, 2935
BseMI GCAATG 3 cut(s) 1321, 1687, 2827
BseMII CTCAG 6 cut(s) 90, 221, 1430, 1797, 2485, 2497
BseNI ACTGG 8 cut(s) 70, 623, 858, 971, 1236, 1927, 2762, 2945
BseRI GAGGAG 1 cut(s) 525
BseSI GKGCMC 1 cut(s) 65
BseXI GCAGC 6 cut(s) 932, 1050, 1123, 2668, 2866, 2869
BseYI CCCAGC 1 cut(s) 2043
BsgI GTGCAG 2 cut(s) 972, 1958
Bsh1236I CGCG 3 cut(s) 87, 241, 1790
BshFI GGCC 6 cut(s) 14, 252, 822, 1320, 2024, 2996
BshNI GGYRCC 1 cut(s) 1258
BshTI ACCGGT 2 cut(s) 1697, 2252
BsiHKAI GWGCWC 2 cut(s) 503, 579
BsiHKCI CYCGRG 1 cut(s) 1995
BsiSI CCGG 6 cut(s) 130, 350, 357, 696, 1698, 2253
BslFI GGGAC 3 cut(s) 997, 1321, 2951
BslI CCNNNNNNNGG 8 cut(s) 260, 356, 391, 646, 1107, 1923, 2219, 2935
BsmAI GTCTC 5 cut(s) 329, 1147, 2143, 2486, 2865
BsmBI CGTCTC 1 cut(s) 2486
BsmFI GGGAC 3 cut(s) 997, 1321, 2951
BsmI GAATGC 2 cut(s) 1417, 2481
BsnI GGCC 6 cut(s) 14, 252, 822, 1320, 2024, 2996
BsoBI CYCGRG 1 cut(s) 1995
Bsp119I TTCGAA 5 cut(s) 301, 2500, 2567, 2881, 2947
Bsp1286I GDGCHC 4 cut(s) 65, 503, 579, 1861
Bsp19I CCATGG 3 cut(s) 1092, 2434, 2694
BspACI CCGC 9 cut(s) 34, 42, 85, 142, 164, 241, 401, 1597, 1917
BspANI GGCC 6 cut(s) 14, 252, 822, 1320, 2024, 2996
BspCNI CTCAG 6 cut(s) 89, 220, 1429, 1798, 2484, 2496
BspFNI CGCG 3 cut(s) 87, 241, 1790
BspLI GGNNCC 7 cut(s) 787, 1260, 1309, 1422, 1759, 2181, 2692
BspMAI CTGCAG 3 cut(s) 1394, 1726, 2686
BspMI ACCTGC 2 cut(s) 994, 1319
BspPI GGATC 3 cut(s) 221, 1897, 2377
BspT104I TTCGAA 5 cut(s) 301, 2500, 2567, 2881, 2947
BspT107I GGYRCC 1 cut(s) 1258
BspTI CTTAAG 1 cut(s) 2419
BsrBI CCGCTC 1 cut(s) 403
BsrDI GCAATG 3 cut(s) 1321, 1687, 2827
BsrFI RCCGGY 3 cut(s) 129, 1697, 2252
BsrI ACTGG 8 cut(s) 70, 623, 858, 971, 1236, 1927, 2762, 2945
BssAI RCCGGY 3 cut(s) 129, 1697, 2252
BssT1I CCWWGG 6 cut(s) 1092, 1333, 2434, 2619, 2626, 2694
Bst4CI ACNGT 4 cut(s) 450, 2083, 2636, 2914
Bst6I CTCTTC 4 cut(s) 131, 1167, 1838, 2840
BstAFI CTTAAG 1 cut(s) 2419
BstAPI GCANNNNNTGC 1 cut(s) 2053
BstBI TTCGAA 5 cut(s) 301, 2500, 2567, 2881, 2947
BstC8I GCNNGC 3 cut(s) 599, 1792, 2785
BstDEI CTNAG 9 cut(s) 76, 207, 884, 1416, 1554, 1806, 2471, 2483, 2583
BstDSI CCRYGG 3 cut(s) 1092, 2434, 2694
BstEII GGTNACC 1 cut(s) 352
BstFNI CGCG 3 cut(s) 87, 241, 1790
BstHHI GCGC 2 cut(s) 89, 610
BstMAI GTCTC 5 cut(s) 329, 1147, 2143, 2486, 2865
BstMWI GCNNNNNNNGC 4 cut(s) 139, 175, 607, 2053
BstNSI RCATGY 3 cut(s) 547, 2289, 2722
BstPI GGTNACC 1 cut(s) 352
BstSFI CTRYAG 7 cut(s) 1278, 1390, 1722, 2407, 2655, 2676, 2682
BstSLI GKGCMC 1 cut(s) 65
BstUI CGCG 3 cut(s) 87, 241, 1790
BstV1I GCAGC 6 cut(s) 932, 1050, 1123, 2668, 2866, 2869
BstV2I GAAGAC 1 cut(s) 759
BstX2I RGATCY 2 cut(s) 1665, 2369
BstYI RGATCY 2 cut(s) 1665, 2369
BsuI GTATCC 1 cut(s) 1623
BsuRI GGCC 6 cut(s) 14, 252, 822, 1320, 2024, 2996
BtgI CCRYGG 3 cut(s) 1092, 2434, 2694
BtgZI GCGATG 1 cut(s) 2528
BtrI CACGTC 1 cut(s) 2188
BtsI GCAGTG 1 cut(s) 1833
BtsIMutI CAGTG 5 cut(s) 166, 446, 1229, 1599, 1833
BveI ACCTGC 2 cut(s) 994, 1319
Cac8I GCNNGC 3 cut(s) 599, 1792, 2785
CaiI CAGNNNCTG 2 cut(s) 673, 2780
CfoI GCGC 2 cut(s) 89, 610
Cfr10I RCCGGY 3 cut(s) 129, 1697, 2252
Cfr13I GGNCC 5 cut(s) 127, 251, 1308, 2623, 2995
CseI GACGC 1 cut(s) 2468
Csp6I GTAC 5 cut(s) 1970, 1992, 2040, 2575, 2664
CspAI ACCGGT 2 cut(s) 1697, 2252
CviQI GTAC 5 cut(s) 1970, 1992, 2040, 2575, 2664
DdeI CTNAG 9 cut(s) 76, 207, 884, 1416, 1554, 1806, 2471, 2483, 2583
DraI TTTAAA 1 cut(s) 868
EaeI YGGCCR 2 cut(s) 12, 820
Eam1104I CTCTTC 4 cut(s) 131, 1167, 1838, 2840
EarI CTCTTC 4 cut(s) 131, 1167, 1838, 2840
Ecl136II GAGCTC 2 cut(s) 501, 577
Eco130I CCWWGG 6 cut(s) 1092, 1333, 2434, 2619, 2626, 2694
Eco24I GRGCYC 3 cut(s) 503, 579, 1861
Eco47I GGWCC 3 cut(s) 127, 1308, 2623
Eco53kI GAGCTC 2 cut(s) 501, 577
Eco57I CTGAAG 3 cut(s) 155, 650, 695
Eco88I CYCGRG 1 cut(s) 1995
Eco91I GGTNACC 1 cut(s) 352
EcoICRI GAGCTC 2 cut(s) 501, 577
EcoO109I RGGNCCY 2 cut(s) 1308, 2995
EcoO65I GGTNACC 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 966
EcoT14I CCWWGG 6 cut(s) 1092, 1333, 2434, 2619, 2626, 2694
EcoT38I GRGCYC 3 cut(s) 503, 579, 1861
ErhI CCWWGG 6 cut(s) 1092, 1333, 2434, 2619, 2626, 2694
Esp3I CGTCTC 1 cut(s) 2486
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FaqI GGGAC 3 cut(s) 997, 1321, 2951
FauI CCCGC 1 cut(s) 1590
FbaI TGATCA 1 cut(s) 960
FblI GTMKAC 1 cut(s) 1281
FriOI GRGCYC 3 cut(s) 503, 579, 1861
FspBI CTAG 2 cut(s) 2205, 2984
GlaI GCGC 2 cut(s) 88, 609
GsaI CCCAGC 1 cut(s) 2047
GsuI CTGGAG 3 cut(s) 53, 863, 875
HaeIII GGCC 6 cut(s) 14, 252, 822, 1320, 2024, 2996
HapII CCGG 6 cut(s) 130, 350, 357, 696, 1698, 2253
HgaI GACGC 1 cut(s) 2468
HhaI GCGC 2 cut(s) 89, 610
Hin6I GCGC 2 cut(s) 87, 608
HinP1I GCGC 2 cut(s) 87, 608
HincII GTYRAC 2 cut(s) 1402, 2917
HindII GTYRAC 2 cut(s) 1402, 2917
HindIII AAGCTT 2 cut(s) 2099, 2950
HpaII CCGG 6 cut(s) 130, 350, 357, 696, 1698, 2253
HphI GGTGA 8 cut(s) 39, 364, 539, 1393, 1583, 1754, 2267, 2642
Hpy166II GTNNAC 6 cut(s) 757, 1282, 1384, 1402, 2727, 2917
Hpy8I GTNNAC 6 cut(s) 757, 1282, 1384, 1402, 2727, 2917
Hpy99I CGWCG 4 cut(s) 26, 158, 235, 929
HpyAV CCTTC 9 cut(s) 118, 830, 1064, 1739, 1825, 1892, 2035, 2152, 2844
HpyCH4III ACNGT 4 cut(s) 450, 2083, 2636, 2914
HpyCH4IV ACGT 3 cut(s) 603, 2187, 2602
HpyF10VI GCNNNNNNNGC 4 cut(s) 139, 175, 607, 2053
HpyF3I CTNAG 9 cut(s) 76, 207, 884, 1416, 1554, 1806, 2471, 2483, 2583
HpySE526I ACGT 3 cut(s) 603, 2187, 2602
HspAI GCGC 2 cut(s) 87, 608
Ksp22I TGATCA 1 cut(s) 960
LmnI GCTCC 4 cut(s) 166, 506, 791, 1000
Lsp1109I GCAGC 6 cut(s) 932, 1050, 1123, 2668, 2866, 2869
LweI GCATC 7 cut(s) 615, 922, 950, 1627, 2505, 2710, 2800
MaeI CTAG 2 cut(s) 2205, 2984
MaeII ACGT 3 cut(s) 603, 2187, 2602
MbiI CCGCTC 1 cut(s) 403
MfeI CAATTG 1 cut(s) 1893
MflI RGATCY 2 cut(s) 1665, 2369
MhlI GDGCHC 4 cut(s) 65, 503, 579, 1861
MlsI TGGCCA 2 cut(s) 14, 822
MluNI TGGCCA 2 cut(s) 14, 822
MlyI GAGTC 5 cut(s) 197, 742, 1114, 1235, 2867
MmeI TCCRAC 2 cut(s) 90, 2933
Mox20I TGGCCA 2 cut(s) 14, 822
MroXI GAANNNNTTC 1 cut(s) 2496
MscI TGGCCA 2 cut(s) 14, 822
MseI TTAA 7 cut(s) 867, 1500, 1511, 2093, 2361, 2420, 2795
MslI CAYNNNNRTG 1 cut(s) 2706
Msp20I TGGCCA 2 cut(s) 14, 822
MspA1I CMGCKG 2 cut(s) 36, 2681
MspCI CTTAAG 1 cut(s) 2419
MspI CCGG 6 cut(s) 130, 350, 357, 696, 1698, 2253
MunI CAATTG 1 cut(s) 1893
Mva1269I GAATGC 2 cut(s) 1417, 2481
MvnI CGCG 3 cut(s) 87, 241, 1790
MwoI GCNNNNNNNGC 4 cut(s) 139, 175, 607, 2053
NcoI CCATGG 3 cut(s) 1092, 2434, 2694
NlaIV GGNNCC 7 cut(s) 787, 1260, 1309, 1422, 1759, 2181, 2692
NmuCI GTSAC 7 cut(s) 352, 444, 1227, 2005, 2603, 2630, 2924
NspI RCATGY 3 cut(s) 547, 2289, 2722
NspV TTCGAA 5 cut(s) 301, 2500, 2567, 2881, 2947
PctI GAATGC 2 cut(s) 1417, 2481
PdmI GAANNNNTTC 1 cut(s) 2496
PflFI GACNNNGTC 1 cut(s) 2636
PflMI CCANNNNNTGG 2 cut(s) 646, 2219
PinAI ACCGGT 2 cut(s) 1697, 2252
PleI GAGTC 5 cut(s) 197, 742, 1114, 1234, 2867
PpsI GAGTC 5 cut(s) 197, 742, 1114, 1234, 2867
PpuMI RGGWCCY 1 cut(s) 1308
PsiI TTATAA 1 cut(s) 2615
Psp124BI GAGCTC 2 cut(s) 503, 579
Psp5II RGGWCCY 1 cut(s) 1308
PspEI GGTNACC 1 cut(s) 352
PspFI CCCAGC 1 cut(s) 2043
PspN4I GGNNCC 7 cut(s) 787, 1260, 1309, 1422, 1759, 2181, 2692
PspPI GGNCC 5 cut(s) 127, 251, 1308, 2623, 2995
PspPPI RGGWCCY 1 cut(s) 1308
PstI CTGCAG 3 cut(s) 1394, 1726, 2686
PstNI CAGNNNCTG 2 cut(s) 673, 2780
PsuI RGATCY 2 cut(s) 1665, 2369
PsyI GACNNNGTC 1 cut(s) 2636
PvuII CAGCTG 1 cut(s) 2681
RsaI GTAC 5 cut(s) 1971, 1993, 2041, 2576, 2665
RsaNI GTAC 5 cut(s) 1970, 1992, 2040, 2575, 2664
RseI CAYNNNNRTG 1 cut(s) 2706
SacI GAGCTC 2 cut(s) 503, 579
SaqAI TTAA 7 cut(s) 867, 1500, 1511, 2093, 2361, 2420, 2795
Sau96I GGNCC 5 cut(s) 127, 251, 1308, 2623, 2995
ScaI AGTACT 1 cut(s) 2665
SchI GAGTC 5 cut(s) 197, 742, 1114, 1235, 2867
SduI GDGCHC 4 cut(s) 65, 503, 579, 1861
SfaNI GCATC 7 cut(s) 615, 922, 950, 1627, 2505, 2710, 2800
SfcI CTRYAG 7 cut(s) 1278, 1390, 1722, 2407, 2655, 2676, 2682
SfuI TTCGAA 5 cut(s) 301, 2500, 2567, 2881, 2947
SinI GGWCC 3 cut(s) 127, 1308, 2623
SmiMI CAYNNNNRTG 1 cut(s) 2706
SmlI CTYRAG 7 cut(s) 146, 578, 611, 1441, 1466, 2419, 2863
SmoI CTYRAG 7 cut(s) 146, 578, 611, 1441, 1466, 2419, 2863
SsiI CCGC 9 cut(s) 34, 42, 85, 142, 164, 241, 401, 1597, 1917
SspI AATATT 3 cut(s) 910, 1435, 1480
SspMI CTAG 2 cut(s) 2205, 2984
SstI GAGCTC 2 cut(s) 503, 579
StyI CCWWGG 6 cut(s) 1092, 1333, 2434, 2619, 2626, 2694
TaaI ACNGT 4 cut(s) 450, 2083, 2636, 2914
TaiI ACGT 3 cut(s) 606, 2190, 2605
TatI WGTACW 1 cut(s) 2663
TauI GCSGC 5 cut(s) 36, 44, 87, 145, 403
Tru1I TTAA 7 cut(s) 867, 1500, 1511, 2093, 2361, 2420, 2795
Tru9I TTAA 7 cut(s) 867, 1500, 1511, 2093, 2361, 2420, 2795
TscAI CASTG 5 cut(s) 166, 453, 1236, 1599, 1833
TseFI GTSAC 7 cut(s) 352, 444, 1227, 2005, 2603, 2630, 2924
TseI GCWGC 6 cut(s) 920, 1038, 1111, 2681, 2854, 2857
Tsp45I GTSAC 7 cut(s) 352, 444, 1227, 2005, 2603, 2630, 2924
TspDTI ATGAA 9 cut(s) 1049, 1154, 1232, 1305, 1374, 1562, 1896, 2180, 2559
TspGWI ACGGA 5 cut(s) 219, 711, 1144, 1991, 2562
TspRI CASTG 5 cut(s) 166, 453, 1236, 1599, 1833
Tth111I GACNNNGTC 1 cut(s) 2636
Van91I CCANNNNNTGG 2 cut(s) 646, 2219
Vha464I CTTAAG 1 cut(s) 2419
VpaK11BI GGWCC 3 cut(s) 127, 1308, 2623
XapI RAATTY 5 cut(s) 294, 966, 1013, 1618, 2493
XceI RCATGY 3 cut(s) 547, 2289, 2722
XmiI GTMKAC 1 cut(s) 1281
XmnI GAANNNNTTC 1 cut(s) 2496
XspI CTAG 2 cut(s) 2205, 2984
ZrmI AGTACT 1 cut(s) 2665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.