AT1G22850

SNARE associated Golgi protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008617
Physical Location & Seq
Reverse (-)
8080482 .. 8082882
2401 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G22850.1

Sequence Viewer

Length: 1035 bp
ATGCGCAGCCTCACTCTAGGTCTGACTCTCAGATCCTCCTCCTCCTCTTCTCTTCCATTCACCTGCAACTCCCGCTTCTGCTCTCCTCCTCCTCCTTCTTCGTCCTTTAGACTCTACAAACGATTCCATTTCCTTAAACCATGCTCTTCCCTAAAGCAAACCAAGAAGAAGAAGCAACAGTCTCTCCCATCTACCGCTCCGCCTCCTCAGAGTCTCCGGTGGTTCTTCAATTCTAAATCCACTAATGATGAAAACGACGAAGATGATGTTAAGTCTGAGAGCGATGATGATGGCGGATCGGAAGGTGATGCTGCTATTAAGGGTACTATCTTAGCCGGAGTTTTGTTGATTGGTACTGTTGGTGGATTCGCCGGCGTTGGATATGTCTACAGGGATCAGATCAATACTTTCCTTACTCAATTCTCCACTTACATCGAAGGTTATGGTACGGCTGGGTATGCGTTATTCATTGCCGTCTATGCTGGGCTTGAGATACTAGCCATTCCAGCTCTCCCATTGACGATGTCGGCTGGTCTTCTCTTCGGTCCACTCATTGGCACTATCATAGTTTCTATCAGTGGAACTATGGCTGCTAGTGTTGCTTTCTTAATTGCTAGATATTTTGCCCGAGAGCGCATCCTTAAGTTAGTTGAAGACAACAAGAAGTTCCTCGCCATTGATAAAGCGATTGGTGAAAATGGGTTTAGAGTTGTTACTCTCCTTCGGCTAAGCCCTCTACTTCCTTTCTCTCTTGGCAATTACCTGTACGGGTTGACGTCTGTGAAGTTTGTGCCTTATGTATTAGGAAGCTGGTTGGGTATGCTTCCAGGCTCTTGGGCTTATGTCAGCGCCGGGGCTTTTGGACGAGCAATCATTCAAGAAGAATCTAATGTTGGCTTGCCTGGAGGAAACGGCCAGCTTTTAACTCTCGGGGTGGGTCTGTTGGTTACTGCATTGGCTGGAACATATGTGACAAGCCTTGCAAAGGATGCTATAAAGGATATTGATGATGATGAGAAGAGAGATGCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

344

Amino Acids

36.9

Weight (kDa)

8.79

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 168 - 286 8.6e-24 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 71
AatII GACGTC 1 cut(s) 779
Acc16I TGCGCA 1 cut(s) 5
Acc36I ACCTGC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 554
AccBSI CCGCTC 1 cut(s) 197
AccI GTMKAC 1 cut(s) 387
AciI CCGC 4 cut(s) 73, 195, 200, 294
AclWI GGATC 3 cut(s) 27, 304, 402
AcoI YGGCCR 1 cut(s) 913
AcyI GRCGYC 1 cut(s) 776
AfaI GTAC 4 cut(s) 325, 355, 448, 767
AfiI CCNNNNNNNGG 2 cut(s) 554, 985
AflII CTTAAG 1 cut(s) 641
AgsI TTSAA 3 cut(s) 229, 653, 878
AjnI CCWGG 2 cut(s) 826, 901
AjuI GAANNNNNNNTTGG 2 cut(s) 876, 908
AluBI AGCT 3 cut(s) 509, 810, 919
AluI AGCT 3 cut(s) 509, 810, 919
Alw26I GTCTC 2 cut(s) 186, 218
AlwI GGATC 3 cut(s) 27, 304, 402
Ama87I CYCGRG 2 cut(s) 627, 929
AoxI GGCC 1 cut(s) 913
ApeKI GCWGC 3 cut(s) 6, 311, 590
AspLEI GCGC 3 cut(s) 6, 636, 851
AspS9I GGNCC 1 cut(s) 545
AsuC2I CCSGG 1 cut(s) 853
AsuHPI GGTGA 3 cut(s) 52, 317, 704
AvaI CYCGRG 2 cut(s) 627, 929
AvaII GGWCC 1 cut(s) 545
BbsI GAAGAC 2 cut(s) 527, 660
BbvI GCAGC 3 cut(s) 18, 298, 577
BccI CCATC 2 cut(s) 196, 284
BceAI ACGGC 3 cut(s) 458, 465, 928
BciT130I CCWGG 2 cut(s) 828, 903
BcnI CCSGG 1 cut(s) 853
BcoDI GTCTC 2 cut(s) 186, 218
BfaI CTAG 4 cut(s) 17, 497, 594, 615
BfmI CTRYAG 1 cut(s) 388
BfoI RGCGCY 1 cut(s) 852
BfrI CTTAAG 1 cut(s) 641
BfuAI ACCTGC 1 cut(s) 71
BisI GCNGC 3 cut(s) 7, 312, 591
BlpI GCTNAGC 1 cut(s) 728
BlsI GCNGC 3 cut(s) 8, 313, 592
Bme1390I CCNGG 3 cut(s) 828, 853, 903
Bme18I GGWCC 1 cut(s) 545
BmeT110I CYCGRG 2 cut(s) 627, 929
BmgT120I GGNCC 1 cut(s) 545
BmrFI CCNGG 3 cut(s) 828, 853, 903
BmsI GCATC 4 cut(s) 298, 645, 979, 1015
BpiI GAAGAC 2 cut(s) 527, 660
BpmI CTGGAG 1 cut(s) 924
Bpu1102I GCTNAGC 1 cut(s) 728
BpuEI CTTGAG 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 853
BsaHI GRCGYC 1 cut(s) 776
BsaJI CCNNGG 1 cut(s) 852
BsaWI WCCGGW 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 2 cut(s) 554, 985
Bse118I RCCGGY 1 cut(s) 371
Bse3DI GCAATG 1 cut(s) 468
BseBI CCWGG 2 cut(s) 828, 903
BseDI CCNNGG 1 cut(s) 852
BseGI GGATG 2 cut(s) 636, 994
BseLI CCNNNNNNNGG 2 cut(s) 554, 985
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 3 cut(s) 43, 221, 267
BseRI GAGGAG 7 cut(s) 28, 31, 34, 75, 78, 81, 195
BseXI GCAGC 3 cut(s) 18, 298, 577
BseYI CCCAGC 2 cut(s) 452, 482
BshFI GGCC 1 cut(s) 915
BsiHKCI CYCGRG 2 cut(s) 627, 929
BsiSI CCGG 4 cut(s) 217, 336, 372, 852
BslI CCNNNNNNNGG 2 cut(s) 554, 985
BsmAI GTCTC 2 cut(s) 186, 218
BsnI GGCC 1 cut(s) 915
BsoBI CYCGRG 2 cut(s) 627, 929
Bsp143I GATC 4 cut(s) 32, 296, 394, 399
Bsp1720I GCTNAGC 1 cut(s) 728
BspACI CCGC 4 cut(s) 73, 195, 200, 294
BspANI GGCC 1 cut(s) 915
BspCNI CTCAG 3 cut(s) 42, 220, 268
BspMI ACCTGC 1 cut(s) 71
BspPI GGATC 3 cut(s) 27, 304, 402
BspQI GCTCTTC 1 cut(s) 151
BspTI CTTAAG 1 cut(s) 641
BsrBI CCGCTC 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 468
BsrFI RCCGGY 1 cut(s) 371
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 1 cut(s) 852
BssMI GATC 4 cut(s) 32, 296, 394, 399
BssNI GRCGYC 1 cut(s) 776
Bst2UI CCWGG 2 cut(s) 828, 903
Bst4CI ACNGT 2 cut(s) 180, 358
Bst6I CTCTTC 5 cut(s) 52, 57, 151, 545, 1013
BstACI GRCGYC 1 cut(s) 776
BstAFI CTTAAG 1 cut(s) 641
BstAPI GCANNNNNTGC 1 cut(s) 989
BstC8I GCNNGC 3 cut(s) 373, 899, 917
BstDEI CTNAG 5 cut(s) 29, 207, 276, 331, 728
BstENI CCTNNNNNAGG 1 cut(s) 983
BstF5I GGATG 2 cut(s) 636, 994
BstH2I RGCGCY 1 cut(s) 852
BstHHI GCGC 3 cut(s) 6, 636, 851
BstKTI GATC 4 cut(s) 35, 299, 397, 402
BstMAI GTCTC 2 cut(s) 186, 218
BstMBI GATC 4 cut(s) 32, 296, 394, 399
BstMWI GCNNNNNNNGC 6 cut(s) 72, 458, 479, 506, 599, 989
BstNI CCWGG 2 cut(s) 828, 903
BstSCI CCNGG 3 cut(s) 826, 851, 901
BstSFI CTRYAG 1 cut(s) 388
BstV1I GCAGC 3 cut(s) 18, 298, 577
BstV2I GAAGAC 2 cut(s) 527, 660
BstX2I RGATCY 1 cut(s) 32
BstXI CCANNNNNNTGG 1 cut(s) 834
BstYI RGATCY 1 cut(s) 32
BsuRI GGCC 1 cut(s) 915
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 2 cut(s) 636, 994
BtsIMutI CAGTG 1 cut(s) 583
BveI ACCTGC 1 cut(s) 71
Cac8I GCNNGC 3 cut(s) 373, 899, 917
CfoI GCGC 3 cut(s) 6, 636, 851
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 1 cut(s) 545
Csp6I GTAC 4 cut(s) 324, 354, 447, 766
CviAII CATG 1 cut(s) 141
CviQI GTAC 4 cut(s) 324, 354, 447, 766
DdeI CTNAG 5 cut(s) 29, 207, 276, 331, 728
DpnI GATC 4 cut(s) 34, 298, 396, 401
DpnII GATC 4 cut(s) 32, 296, 394, 399
EaeI YGGCCR 1 cut(s) 913
Eam1104I CTCTTC 5 cut(s) 52, 57, 151, 545, 1013
EarI CTCTTC 5 cut(s) 52, 57, 151, 545, 1013
EciI GGCGGA 2 cut(s) 189, 309
Eco47I GGWCC 1 cut(s) 545
Eco88I CYCGRG 2 cut(s) 627, 929
EcoNI CCTNNNNNAGG 1 cut(s) 983
EcoRII CCWGG 2 cut(s) 826, 901
FaeI CATG 1 cut(s) 144
FatI CATG 1 cut(s) 140
FauI CCCGC 1 cut(s) 80
FauNDI CATATG 1 cut(s) 967
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 3 cut(s) 7, 312, 591
FokI GGATG 2 cut(s) 623, 1001
Fsp4HI GCNGC 3 cut(s) 7, 312, 591
FspBI CTAG 4 cut(s) 17, 497, 594, 615
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 635, 850
GluI GCNGC 3 cut(s) 7, 312, 591
GsaI CCCAGC 2 cut(s) 456, 486
GsuI CTGGAG 1 cut(s) 924
HaeII RGCGCY 1 cut(s) 852
HaeIII GGCC 1 cut(s) 915
HapII CCGG 4 cut(s) 217, 336, 372, 852
HhaI GCGC 3 cut(s) 6, 636, 851
Hin1I GRCGYC 1 cut(s) 776
Hin1II CATG 1 cut(s) 144
Hin6I GCGC 3 cut(s) 4, 634, 849
HinP1I GCGC 3 cut(s) 4, 634, 849
HincII GTYRAC 1 cut(s) 774
HindII GTYRAC 1 cut(s) 774
HinfI GANTC 6 cut(s) 25, 111, 123, 211, 366, 884
HpaII CCGG 4 cut(s) 217, 336, 372, 852
HphI GGTGA 3 cut(s) 52, 317, 704
Hpy166II GTNNAC 3 cut(s) 388, 548, 774
Hpy188I TCNGA 6 cut(s) 24, 32, 210, 277, 301, 399
Hpy188III TCNNGA 1 cut(s) 878
Hpy8I GTNNAC 3 cut(s) 388, 548, 774
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 4 cut(s) 105, 296, 431, 731
HpyCH4III ACNGT 2 cut(s) 180, 358
HpyCH4IV ACGT 1 cut(s) 776
HpyCH4V TGCA 4 cut(s) 66, 953, 983, 1028
HpyF10VI GCNNNNNNNGC 6 cut(s) 72, 458, 479, 506, 599, 989
HpyF3I CTNAG 5 cut(s) 29, 207, 276, 331, 728
HpySE526I ACGT 1 cut(s) 776
Hsp92I GRCGYC 1 cut(s) 776
Hsp92II CATG 1 cut(s) 144
HspAI GCGC 3 cut(s) 4, 634, 849
KroI GCCGGC 1 cut(s) 371
KroNI GCCGGC 1 cut(s) 373
Kzo9I GATC 4 cut(s) 32, 296, 394, 399
LguI GCTCTTC 1 cut(s) 151
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 3 cut(s) 18, 298, 577
LweI GCATC 4 cut(s) 298, 645, 979, 1015
MaeI CTAG 4 cut(s) 17, 497, 594, 615
MaeII ACGT 1 cut(s) 776
MaeIII GTNAC 3 cut(s) 712, 946, 970
MalI GATC 4 cut(s) 34, 298, 396, 401
MbiI CCGCTC 1 cut(s) 197
MboI GATC 4 cut(s) 32, 296, 394, 399
MflI RGATCY 1 cut(s) 32
MluCI AATT 4 cut(s) 229, 419, 609, 757
MlyI GAGTC 3 cut(s) 19, 105, 220
MmeI TCCRAC 1 cut(s) 358
MreI CGCCGGCG 1 cut(s) 371
MroNI GCCGGC 1 cut(s) 371
MseI TTAA 6 cut(s) 135, 270, 318, 608, 642, 923
MspCI CTTAAG 1 cut(s) 641
MspI CCGG 4 cut(s) 217, 336, 372, 852
MspR9I CCNGG 3 cut(s) 828, 853, 903
MvaI CCWGG 2 cut(s) 828, 903
MwoI GCNNNNNNNGC 6 cut(s) 72, 458, 479, 506, 599, 989
NaeI GCCGGC 1 cut(s) 373
NciI CCSGG 1 cut(s) 853
NdeI CATATG 1 cut(s) 967
NdeII GATC 4 cut(s) 32, 296, 394, 399
NgoMIV GCCGGC 1 cut(s) 371
NlaIII CATG 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 970
NsbI TGCGCA 1 cut(s) 5
PaqCI CACCTGC 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 151
PdiI GCCGGC 1 cut(s) 373
PfeI GAWTC 3 cut(s) 123, 366, 884
PflFI GACNNNGTC 1 cut(s) 523
PflMI CCANNNNNTGG 1 cut(s) 554
PkrI GCNGC 3 cut(s) 8, 313, 592
PleI GAGTC 3 cut(s) 19, 105, 219
PpsI GAGTC 3 cut(s) 19, 105, 219
Psp6I CCWGG 2 cut(s) 826, 901
PspFI CCCAGC 2 cut(s) 452, 482
PspGI CCWGG 2 cut(s) 826, 901
PspPI GGNCC 1 cut(s) 545
PsuI RGATCY 1 cut(s) 32
PsyI GACNNNGTC 1 cut(s) 523
RsaI GTAC 4 cut(s) 325, 355, 448, 767
RsaNI GTAC 4 cut(s) 324, 354, 447, 766
SapI GCTCTTC 1 cut(s) 151
SaqAI TTAA 6 cut(s) 135, 270, 318, 608, 642, 923
SatI GCNGC 3 cut(s) 7, 312, 591
Sau3AI GATC 4 cut(s) 32, 296, 394, 399
Sau96I GGNCC 1 cut(s) 545
SchI GAGTC 3 cut(s) 19, 105, 220
ScrFI CCNGG 3 cut(s) 828, 853, 903
SetI ASST 9 cut(s) 22, 65, 307, 442, 511, 765, 779, 812, 921
SfaNI GCATC 4 cut(s) 298, 645, 979, 1015
SfcI CTRYAG 1 cut(s) 388
SgrAI CRCCGGYG 1 cut(s) 371
SinI GGWCC 1 cut(s) 545
SmlI CTYRAG 2 cut(s) 488, 641
SmoI CTYRAG 2 cut(s) 488, 641
Sse9I AATT 4 cut(s) 229, 419, 609, 757
SsiI CCGC 4 cut(s) 73, 195, 200, 294
SspMI CTAG 4 cut(s) 17, 497, 594, 615
StyD4I CCNGG 3 cut(s) 826, 851, 901
TaaI ACNGT 2 cut(s) 180, 358
TaiI ACGT 1 cut(s) 779
TaqI TCGA 1 cut(s) 435
TaqII GACCGA 1 cut(s) 533
TasI AATT 4 cut(s) 229, 419, 609, 757
TfiI GAWTC 3 cut(s) 123, 366, 884
Tru1I TTAA 6 cut(s) 135, 270, 318, 608, 642, 923
Tru9I TTAA 6 cut(s) 135, 270, 318, 608, 642, 923
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 1 cut(s) 970
TseI GCWGC 3 cut(s) 6, 311, 590
Tsp45I GTSAC 1 cut(s) 970
TspDTI ATGAA 2 cut(s) 264, 457
TspRI CASTG 1 cut(s) 583
Tth111I GACNNNGTC 1 cut(s) 523
Van91I CCANNNNNTGG 1 cut(s) 554
Vha464I CTTAAG 1 cut(s) 641
VpaK11BI GGWCC 1 cut(s) 545
XagI CCTNNNNNAGG 1 cut(s) 983
XmiI GTMKAC 1 cut(s) 387
XspI CTAG 4 cut(s) 17, 497, 594, 615
ZraI GACGTC 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.