RchiOBHm_Chr6g0251261

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008617
Physical Location & Seq
Forward (+)
6551430 .. 6557129
5700 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22526

Sequence Viewer

Length: 1005 bp
ATGCGCACCCTCTTCCTCACTCTGAGGCCACCACCACCTTCTCCGCCGTGCCTCTCTCCCTTCTCCTCCTCCTCCTCCCACCCTTCCTTCCTCTTCAAACCCAACCACCACCGCTTCCACTTCCTCTCGCCATGCTCCTCTCTCAAGCAGACCAAGAACAAGACCAGCCGCCTCCAGAAGACCACCACTGCTCCGCCGCAGAGCCTCAAGTGGCTCTTCAATAATCCCAAGATTGACGACGGCGGCGAGATTGAAAAGGGTAGCGGCGGCGGCGGTGGCGGTGATGATGAGGGCGAGGGTGGGTTGGCGCTTAAAGGTACTGTCTTGGCGGGGCTGTTGTTGGTTGGGCTTGTTGGTGGGTTTGGGGCGGCTGGGTATGTGTACAAGGATCAGGTTAATGCCTTCTTGAATCAGTTTACGACTCTCATTGAAGGGTATGGTCCAGCGGGATATGCTTTGTTTGTAGCAGTTTATGCAGGACTGGAAATTCTTGCGATTCCAGCTGTTCCATTGACCATGTCAGCTGGTCTTCTTTTTGGCACAGTTACCGGATCAATTCTAGTCTCTCTAAGCGGAACGGTGGCTGCTAGCGTTGCTTTTCTGATTGCTAGATATTTTGCTCGTGAGCGTATTCTTAAAATGGTTGAGGGAAACAAAAAGTTTCTTGCAATTGATAAGGCTATTGGAGAAAATGGCTTCAGAGTTGTTACTCTCCTCCGTTTGAGCCCTTTGCTTCCTTTTTCTCTGGGGAACTATTTATATGGATTGACATCTGTAAAGTTTGTCCCATATGTGTTGGGAAGTTGGTTGGGGATGCTTCCAGGAACATGGGCTTATGTGAGTGCTGGTGCATTTGGGCGAGCCATAATTCAAGAGGAATCTGATGTTGGTTTGCCCGGAGGAAATGGTCAGCTGTTGACACTAGCAGCTGGACTGTTAGTCACAGCATTGGCTGCTGCTTACGTAACACGATTAGCAAAGGATGCTGTAAAAGATATCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

334

Amino Acids

35.16

Weight (kDa)

9.3

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 166 - 284 1.8e-23 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AclWI GGATC 2 cut(s) 396, 559
AcsI RAATTY 1 cut(s) 486
AcuI CTGAAG 1 cut(s) 682
AfaI GTAC 2 cut(s) 319, 383
AgsI TTSAA 6 cut(s) 97, 220, 254, 409, 431, 872
AhdI GACNNNNNGTC 1 cut(s) 938
AjnI CCWGG 1 cut(s) 820
AjuI GAANNNNNNNTTGG 2 cut(s) 870, 902
AluBI AGCT 4 cut(s) 503, 524, 913, 929
AluI AGCT 4 cut(s) 503, 524, 913, 929
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 2 cut(s) 396, 559
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 4 cut(s) 584, 926, 953, 956
ApoI RAATTY 1 cut(s) 486
AspLEI GCGC 2 cut(s) 6, 310
AspS9I GGNCC 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 897
AsuHPI GGTGA 1 cut(s) 293
AsuNHI GCTAGC 1 cut(s) 587
AvaII GGWCC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 728
BauI CACGAG 1 cut(s) 621
BbsI GAAGAC 2 cut(s) 185, 521
BbvI GCAGC 4 cut(s) 571, 938, 940, 943
BceAI ACGGC 2 cut(s) 31, 256
BciT130I CCWGG 1 cut(s) 822
BcnI CCSGG 1 cut(s) 897
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 4 cut(s) 560, 588, 609, 923
BfoI RGCGCY 1 cut(s) 311
Bme1390I CCNGG 2 cut(s) 822, 897
Bme18I GGWCC 1 cut(s) 440
BmeRI GACNNNNNGTC 1 cut(s) 938
BmgT120I GGNCC 1 cut(s) 440
BmrFI CCNGG 2 cut(s) 822, 897
BmsI GCATC 2 cut(s) 804, 973
BmtI GCTAGC 1 cut(s) 591
BpiI GAAGAC 2 cut(s) 185, 521
BpmI CTGGAG 1 cut(s) 158
BpuEI CTTGAG 2 cut(s) 128, 191
BpuMI CCSGG 1 cut(s) 897
Bsa29I ATCGAT 1 cut(s) 999
BsaAI YACGTR 1 cut(s) 964
BsaBI GATNNNNATC 1 cut(s) 769
BsaWI WCCGGW 1 cut(s) 548
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bse1I ACTGG 1 cut(s) 486
Bse8I GATNNNNATC 1 cut(s) 769
BseBI CCWGG 1 cut(s) 822
BseCI ATCGAT 1 cut(s) 999
BseGI GGATG 2 cut(s) 819, 988
BseJI GATNNNNATC 1 cut(s) 769
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 486
BseRI GAGGAG 6 cut(s) 55, 58, 61, 64, 127, 704
BseXI GCAGC 4 cut(s) 571, 938, 940, 943
BseYI CCCAGC 1 cut(s) 371
BshFI GGCC 1 cut(s) 28
BshVI ATCGAT 1 cut(s) 999
BsiSI CCGG 2 cut(s) 549, 897
BslFI GGGAC 1 cut(s) 770
BsmAI GTCTC 1 cut(s) 568
BsmFI GGGAC 1 cut(s) 770
BsnI GGCC 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 728
Bsp1407I TGTACA 1 cut(s) 381
Bsp143I GATC 2 cut(s) 388, 551
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 1 cut(s) 15
BspDI ATCGAT 1 cut(s) 999
BspOI GCTAGC 1 cut(s) 591
BspPI GGATC 2 cut(s) 396, 559
BspQI GCTCTTC 1 cut(s) 221
BsrGI TGTACA 1 cut(s) 381
BsrI ACTGG 1 cut(s) 486
BssMI GATC 2 cut(s) 388, 551
BssSI CACGAG 1 cut(s) 621
Bst2BI CACGAG 1 cut(s) 621
Bst2UI CCWGG 1 cut(s) 822
Bst4CI ACNGT 4 cut(s) 322, 544, 580, 936
Bst6I CTCTTC 3 cut(s) 17, 98, 221
BstAPI GCANNNNNTGC 3 cut(s) 473, 953, 983
BstAUI TGTACA 1 cut(s) 381
BstBAI YACGTR 1 cut(s) 964
BstC8I GCNNGC 2 cut(s) 589, 861
BstDEI CTNAG 2 cut(s) 23, 569
BstF5I GGATG 2 cut(s) 819, 988
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 2 cut(s) 6, 310
BstKTI GATC 2 cut(s) 391, 554
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 2 cut(s) 388, 551
BstMWI GCNNNNNNNGC 8 cut(s) 270, 276, 452, 473, 500, 593, 953, 983
BstNI CCWGG 1 cut(s) 822
BstSCI CCNGG 2 cut(s) 820, 895
BstSNI TACGTA 1 cut(s) 964
BstV1I GCAGC 4 cut(s) 571, 938, 940, 943
BstV2I GAAGAC 2 cut(s) 185, 521
BstXI CCANNNNNNTGG 1 cut(s) 828
Bsu15I ATCGAT 1 cut(s) 999
BsuRI GGCC 1 cut(s) 28
BsuTUI ATCGAT 1 cut(s) 999
BtsCI GGATG 2 cut(s) 819, 988
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 2 cut(s) 589, 861
CfoI GCGC 2 cut(s) 6, 310
Cfr13I GGNCC 1 cut(s) 440
ClaI ATCGAT 1 cut(s) 999
Csp6I GTAC 2 cut(s) 318, 382
CviAII CATG 3 cut(s) 132, 517, 828
CviQI GTAC 2 cut(s) 318, 382
DdeI CTNAG 2 cut(s) 23, 569
DpnI GATC 2 cut(s) 390, 553
DpnII GATC 2 cut(s) 388, 551
DriI GACNNNNNGTC 1 cut(s) 938
Eam1104I CTCTTC 3 cut(s) 17, 98, 221
Eam1105I GACNNNNNGTC 1 cut(s) 938
EarI CTCTTC 3 cut(s) 17, 98, 221
EciI GGCGGA 2 cut(s) 33, 183
Eco105I TACGTA 1 cut(s) 964
Eco24I GRGCYC 1 cut(s) 728
Eco32I GATATC 1 cut(s) 997
Eco47I GGWCC 1 cut(s) 440
Eco57I CTGAAG 1 cut(s) 682
EcoRII CCWGG 1 cut(s) 820
EcoRV GATATC 1 cut(s) 997
EcoT38I GRGCYC 1 cut(s) 728
FaeI CATG 3 cut(s) 135, 520, 831
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 770
FatI CATG 3 cut(s) 131, 516, 827
FauI CCCGC 2 cut(s) 322, 439
FauNDI CATATG 1 cut(s) 790
FokI GGATG 2 cut(s) 826, 995
FriOI GRGCYC 1 cut(s) 728
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 4 cut(s) 560, 588, 609, 923
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 2 cut(s) 5, 309
GsaI CCCAGC 1 cut(s) 375
GsuI CTGGAG 1 cut(s) 158
HaeII RGCGCY 1 cut(s) 311
HaeIII GGCC 1 cut(s) 28
HapII CCGG 2 cut(s) 549, 897
HhaI GCGC 2 cut(s) 6, 310
Hin1II CATG 3 cut(s) 135, 520, 831
Hin6I GCGC 2 cut(s) 4, 308
HinP1I GCGC 2 cut(s) 4, 308
HincII GTYRAC 1 cut(s) 918
HindII GTYRAC 1 cut(s) 918
HinfI GANTC 4 cut(s) 409, 421, 496, 878
HpaII CCGG 2 cut(s) 549, 897
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 3 cut(s) 382, 417, 918
Hpy188I TCNGA 4 cut(s) 24, 603, 701, 883
Hpy188III TCNNGA 4 cut(s) 175, 406, 623, 872
Hpy8I GTNNAC 3 cut(s) 382, 417, 918
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 6 cut(s) 48, 70, 93, 97, 412, 425
HpyCH4III ACNGT 4 cut(s) 322, 544, 580, 936
HpyCH4IV ACGT 1 cut(s) 963
HpyCH4V TGCA 3 cut(s) 476, 668, 851
HpyF10VI GCNNNNNNNGC 8 cut(s) 270, 276, 452, 473, 500, 593, 953, 983
HpyF3I CTNAG 2 cut(s) 23, 569
HpySE526I ACGT 1 cut(s) 963
Hsp92II CATG 3 cut(s) 135, 520, 831
HspAI GCGC 2 cut(s) 4, 308
Kzo9I GATC 2 cut(s) 388, 551
LguI GCTCTTC 1 cut(s) 221
LmnI GCTCC 2 cut(s) 140, 196
Lsp1109I GCAGC 4 cut(s) 571, 938, 940, 943
LweI GCATC 2 cut(s) 804, 973
MaeI CTAG 4 cut(s) 560, 588, 609, 923
MaeII ACGT 1 cut(s) 963
MaeIII GTNAC 4 cut(s) 544, 706, 940, 964
MalI GATC 2 cut(s) 390, 553
MboI GATC 2 cut(s) 388, 551
MboII GAAGA 5 cut(s) 4, 85, 190, 208, 521
MfeI CAATTG 1 cut(s) 669
MhlI GDGCHC 1 cut(s) 728
MluCI AATT 4 cut(s) 486, 555, 669, 867
MlyI GAGTC 1 cut(s) 415
MseI TTAA 3 cut(s) 312, 396, 636
MspA1I CMGCKG 5 cut(s) 446, 503, 524, 913, 929
MspI CCGG 2 cut(s) 549, 897
MspR9I CCNGG 2 cut(s) 822, 897
MunI CAATTG 1 cut(s) 669
MvaI CCWGG 1 cut(s) 822
MwoI GCNNNNNNNGC 8 cut(s) 270, 276, 452, 473, 500, 593, 953, 983
NciI CCSGG 1 cut(s) 897
NdeI CATATG 1 cut(s) 790
NdeII GATC 2 cut(s) 388, 551
NheI GCTAGC 1 cut(s) 587
NlaIII CATG 3 cut(s) 135, 520, 831
NmuCI GTSAC 1 cut(s) 940
NsbI TGCGCA 1 cut(s) 5
PciSI GCTCTTC 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PfeI GAWTC 3 cut(s) 409, 496, 878
PflFI GACNNNGTC 1 cut(s) 517
PfoI TCCNGGA 1 cut(s) 820
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
Ppu21I YACGTR 1 cut(s) 964
Psp6I CCWGG 1 cut(s) 820
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 1 cut(s) 820
PspPI GGNCC 1 cut(s) 440
PsyI GACNNNGTC 1 cut(s) 517
PvuII CAGCTG 4 cut(s) 503, 524, 913, 929
RsaI GTAC 2 cut(s) 319, 383
RsaNI GTAC 2 cut(s) 318, 382
SapI GCTCTTC 1 cut(s) 221
SaqAI TTAA 3 cut(s) 312, 396, 636
Sau3AI GATC 2 cut(s) 388, 551
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 2 cut(s) 822, 897
SduI GDGCHC 1 cut(s) 728
SetI ASST 8 cut(s) 40, 319, 396, 505, 526, 915, 931, 966
SfaNI GCATC 2 cut(s) 804, 973
SinI GGWCC 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 143, 206
SmoI CTYRAG 2 cut(s) 143, 206
SnaBI TACGTA 1 cut(s) 964
Sse9I AATT 4 cut(s) 486, 555, 669, 867
SspMI CTAG 4 cut(s) 560, 588, 609, 923
StyD4I CCNGG 2 cut(s) 820, 895
TaaI ACNGT 4 cut(s) 322, 544, 580, 936
TaiI ACGT 1 cut(s) 966
TaqI TCGA 1 cut(s) 999
TasI AATT 4 cut(s) 486, 555, 669, 867
TatI WGTACW 1 cut(s) 381
TauI GCSGC 7 cut(s) 171, 199, 246, 267, 270, 273, 371
TfiI GAWTC 3 cut(s) 409, 496, 878
Tru1I TTAA 3 cut(s) 312, 396, 636
Tru9I TTAA 3 cut(s) 312, 396, 636
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 1 cut(s) 940
TseI GCWGC 4 cut(s) 584, 926, 953, 956
Tsp45I GTSAC 1 cut(s) 940
TspGWI ACGGA 1 cut(s) 707
TspRI CASTG 1 cut(s) 193
Tth111I GACNNNGTC 1 cut(s) 517
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 486
XspI CTAG 4 cut(s) 560, 588, 609, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.