Rorug05G0501300

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008617
Physical Location & Seq
Forward (+)
68249680 .. 68251586
1907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0501300.1

Sequence Viewer

Length: 1734 bp
ATGCCATTTCTACACCCTACTCCTCCTCTTGTTGCTTTGCTTCATTCTCGATCCTCAAAGCCAACCAAAGCTAGAGAAACACAGCTAGGAAAGCGACCCTCAAAGTCCAATGTGAGTAATGTTGAGAATGCCTTGATGGTGTTCGATGAAATGCTTCACTCGCGTCCTCTGCCGTCTGTTGTCCTCTTCAATCAAATCTTGACTCAACTTTCCAAATTGAAACACTATTCGGCCGTTATCTCTTTGAATACACAAATGGGTCTCATCGGAATCGCTTCTAATGCTTATACTCTAAATATTATCATTAATTGCTACTGCCATTTGAATCATTTGGGGTTTGGCATATCTGTCTTGGGTCAATTCTTTAAATTGGGTCTTCAACCAAGTGTCACCACCTTCAACACTCTAATCCACGGCTTTGTTCTCCAGAATAGAGTGGCTGAGGCAGCACGACTTTTCAGCAAAATGGTGGGGCGAGGTCATTGTCAGCCGGATGTGTTTACTTTCAACACACTAATAAAGGGCTTTTGCATGATTGGAAACAACAGTGCAGCTATGGAATTACTTGGGAAGATGGAAGAAAGAGGATGCGAGCCTAACATAGTTTCCTATAGCACCATCATCGACAGTCATTGTAAGGATACACTAGTTGATGAAGCATTGAACCTCTTCACAGAAATGATTGGTAGAGGTATTGCTCCCAACGTTGTTACTTACAACTCTTTGATTCAAGGAGTCTGCAATATCGGCCAGTGGAAAGAAGCTACAAGGTTGTTGAATGAAATGGTGAGTCAAAATATCTTTCCCGATGTAGTCACCTTCAATGTCTTGGTTGATGCACTTTGTAAGGAGGGGATGGTTGTGGAAGCCAAAAGTGTGGTTGAGATGATGGTTCAAAGACATATTGAGCCTGATATCATTACTTACAACTCACTTATGGACGGTTACTGTTTGCGAGGAGAAATGGACGAGGCGAAACAAGTCTTTGATCTAATGGTTAGGAAGGGCTCCATGGTTAATGTTCGGAGTTGTAACATATTGATAAACGGATACTGTAAGCACAAAAAGATTGATGAGGCCAATAAGGTTTTTCAGGAAATGTGTCGCATGGGACTTGTTCCTAATACCGTTACTTATAACACTCTTATCGATGGTTTTTACAAAGCAGGGAGAATACAAGAGGCAGAAAAGTTGTTCTCTGAGATGCAAGGTTGTGGCCAATTTCCAGATCGTCAAATTTATAATATTATACTTGATGGCCTGTGTAACAACCAACAACTTTCTACAGCACTAGAATTACTTAGAGAGATAGAAGGCAACAAGTTGGAACTAGATATTGTAGTTTACAATATTATGATTGAAGGTTTGTGCAAAGCTGGGAACATGGATTCTGCAATGGATGTCTTCTCTGGTTTGTCATTGAAAGGAGTACAGCCTAATGTGAGGACATACAATATAATGATTCATGGATTTTGTAAAGGGGGCTTATTAAGTGAAGCAGAAAAGTTGCTGAGAGAAATGGGAGAGAAAGGTTGCTCTCCAGATGGTTGGACCTATAACATCATTATCCGAGGGTTGTTAAATAACAACGAGGCATCAATGGCTATGAAACTTATTCAAAAAATGGTAGAGTTGGGTTTCTCTGCAGATGCATCAACTATGGAATTGATAGTTGGTTTACTGTCTATGGATATAGTAGATCCGGCTTTGTTGCTGTTGTTAAAAGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

577

Amino Acids

64.13

Weight (kDa)

6.1

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 58 - 107 6.8e-06 PPR repeat family
PPR_3 PF13812 123 - 174 1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 125 - 157 2e-08 PPR repeat
PPR_2 PF13041 128 - 177 1.2e-14 PPR repeat family
PPR_3 PF13812 152 - 209 1.3e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 162 - 193 5.8e-11 PPR repeat
PPR_2 PF13041 164 - 213 2.4e-16 PPR repeat family
PPR PF01535 167 - 197 2e-06 PPR repeat
PPR_1 PF12854 195 - 227 3.8e-12 PPR repeat
PPR_2 PF13041 199 - 248 4e-17 PPR repeat family
PPR_long PF17177 200 - 313 6.9e-08 Pentacotripeptide-repeat region of PRORP
PPR PF01535 202 - 232 2.1e-06 PPR repeat
PPR_3 PF13812 223 - 281 1.6e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 230 - 263 5.7e-12 PPR repeat
PPR_2 PF13041 234 - 283 3.3e-19 PPR repeat family
PPR PF01535 237 - 267 1.2e-06 PPR repeat
MRP-S27 PF10037 238 - 300 3.4e-06 Mitochondrial 28S ribosomal protein S27
PPR_1 PF12854 266 - 298 2.8e-10 PPR repeat
PPR_2 PF13041 269 - 317 1.4e-15 PPR repeat family
PPR PF01535 272 - 301 2.6e-06 PPR repeat
PPR_3 PF13812 292 - 336 4.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 301 - 333 4.6e-12 PPR repeat
PPR_2 PF13041 304 - 349 3.1e-12 PPR repeat family
PPR PF01535 307 - 336 3e-09 PPR repeat
PPR_3 PF13812 328 - 384 9.1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 336 - 367 5.7e-08 PPR repeat
PPR_2 PF13041 340 - 388 2.5e-18 PPR repeat family
PPR PF01535 343 - 372 9.4e-09 PPR repeat
TPR_24 PF23276 353 - 470 3e-10 Fungal tetratrico peptide repeats
PPR_3 PF13812 362 - 419 2.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 370 - 403 1.6e-13 PPR repeat
PPR_2 PF13041 374 - 423 2e-16 PPR repeat family
PPR PF01535 377 - 406 1.5e-09 PPR repeat
PPR_3 PF13812 433 - 489 4.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 442 - 469 1.1e-09 PPR repeat
PPR_2 PF13041 445 - 493 2.8e-18 PPR repeat family
PPR PF01535 447 - 476 3e-06 PPR repeat
PPR_1 PF12854 475 - 507 6e-15 PPR repeat
PPR_3 PF13812 475 - 524 9.8e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 483 - 527 9.5e-16 PPR repeat family
PPR PF01535 483 - 512 6.2e-10 PPR repeat
PPR_2 PF13041 518 - 554 3.5e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1137, 1242
AccII CGCG 1 cut(s) 163
AclI AACGTT 1 cut(s) 705
AclWI GGATC 2 cut(s) 45, 1694
AcoI YGGCCR 3 cut(s) 231, 748, 1216
AcsI RAATTY 1 cut(s) 1236
AfaI GTAC 1 cut(s) 1431
AhlI ACTAGT 1 cut(s) 646
AjuI GAANNNNNNNTTGG 2 cut(s) 1656, 1688
AluBI AGCT 5 cut(s) 71, 85, 554, 764, 1376
AluI AGCT 5 cut(s) 71, 85, 554, 764, 1376
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 2 cut(s) 45, 1694
AoxI GGCC 5 cut(s) 231, 748, 1077, 1216, 1258
ApeKI GCWGC 2 cut(s) 446, 551
ApoI RAATTY 1 cut(s) 1236
AseI ATTAAT 1 cut(s) 306
Asp700I GAANNNNTTC 3 cut(s) 153, 274, 668
AspS9I GGNCC 1 cut(s) 1551
AsuHPI GGTGA 3 cut(s) 382, 799, 808
AvaII GGWCC 1 cut(s) 1551
BaeI ACNNNNGTAYC 2 cut(s) 633, 666
BalI TGGCCA 1 cut(s) 1218
BanII GRGCYC 1 cut(s) 1010
BbsI GAAGAC 2 cut(s) 368, 1396
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 2 cut(s) 458, 563
BccI CCATC 8 cut(s) 130, 568, 626, 850, 883, 1145, 1250, 1538
BceAI ACGGC 3 cut(s) 157, 218, 430
BcgI CGANNNNNNTGC 2 cut(s) 604, 638
BciVI GTATCC 2 cut(s) 634, 1043
BcoDI GTCTC 1 cut(s) 266
BcuI ACTAGT 1 cut(s) 646
BfaI CTAG 5 cut(s) 72, 86, 647, 1292, 1331
BfmI CTRYAG 3 cut(s) 610, 1284, 1644
BfuI GTATCC 2 cut(s) 634, 1043
BisI GCNGC 2 cut(s) 447, 552
BlsI GCNGC 2 cut(s) 448, 553
Bme18I GGWCC 1 cut(s) 1551
BmgT120I GGNCC 1 cut(s) 1551
BmiI GGNNCC 1 cut(s) 1009
BmsI GCATC 6 cut(s) 578, 826, 1194, 1604, 1639, 1661
BpiI GAAGAC 2 cut(s) 368, 1396
BpmI CTGGAG 2 cut(s) 410, 1524
Bpu10I CCTNAGC 1 cut(s) 441
Bsa29I ATCGAT 1 cut(s) 1149
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 3 cut(s) 412, 1011, 1570
Bse1I ACTGG 1 cut(s) 751
Bse3DI GCAATG 1 cut(s) 1401
BseCI ATCGAT 1 cut(s) 1149
BseDI CCNNGG 3 cut(s) 412, 1011, 1570
BseGI GGATG 4 cut(s) 499, 593, 861, 1405
BseMI GCAATG 1 cut(s) 1401
BseMII CTCAG 3 cut(s) 432, 1191, 1502
BseNI ACTGG 1 cut(s) 751
BseRI GAGGAG 3 cut(s) 12, 15, 972
BseX3I CGGCCG 1 cut(s) 231
BseXI GCAGC 2 cut(s) 458, 563
BseYI CCCAGC 1 cut(s) 1376
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 163
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 5 cut(s) 233, 750, 1079, 1218, 1260
BshVI ATCGAT 1 cut(s) 1149
BsiEI CGRYCG 1 cut(s) 234
BsiSI CCGG 2 cut(s) 491, 1703
BslFI GGGAC 1 cut(s) 1125
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 1125
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 5 cut(s) 233, 750, 1079, 1218, 1260
Bso31I GGTCTC 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 1010
Bsp143I GATC 4 cut(s) 50, 988, 1228, 1699
Bsp19I CCATGG 1 cut(s) 1011
BspANI GGCC 5 cut(s) 233, 750, 1079, 1218, 1260
BspCNI CTCAG 3 cut(s) 433, 1192, 1503
BspDI ATCGAT 1 cut(s) 1149
BspFNI CGCG 1 cut(s) 163
BspHI TCATGA 1 cut(s) 1730
BspLI GGNNCC 1 cut(s) 1009
BspMAI CTGCAG 1 cut(s) 1648
BspPI GGATC 2 cut(s) 45, 1694
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 1401
BsrI ACTGG 1 cut(s) 751
BssECI CCNNGG 3 cut(s) 412, 1011, 1570
BssMI GATC 4 cut(s) 50, 988, 1228, 1699
BssT1I CCWWGG 1 cut(s) 1011
Bst4CI ACNGT 7 cut(s) 548, 629, 944, 950, 1055, 1129, 1683
Bst6I CTCTTC 2 cut(s) 191, 674
BstC8I GCNNGC 1 cut(s) 593
BstDEI CTNAG 4 cut(s) 441, 1200, 1301, 1511
BstDSI CCRYGG 2 cut(s) 412, 1011
BstF5I GGATG 4 cut(s) 499, 593, 861, 1405
BstFNI CGCG 1 cut(s) 163
BstKTI GATC 4 cut(s) 53, 991, 1231, 1702
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 4 cut(s) 50, 988, 1228, 1699
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 7 cut(s) 91, 160, 169, 281, 446, 747, 1601
BstSFI CTRYAG 3 cut(s) 610, 1284, 1644
BstUI CGCG 1 cut(s) 163
BstV1I GCAGC 2 cut(s) 458, 563
BstV2I GAAGAC 2 cut(s) 368, 1396
BstX2I RGATCY 1 cut(s) 1699
BstXI CCANNNNNNTGG 2 cut(s) 877, 1548
BstYI RGATCY 1 cut(s) 1699
BstZI CGGCCG 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 1149
BsuI GTATCC 2 cut(s) 634, 1043
BsuRI GGCC 5 cut(s) 233, 750, 1079, 1218, 1260
BsuTUI ATCGAT 1 cut(s) 1149
BtgI CCRYGG 2 cut(s) 412, 1011
BtsCI GGATG 4 cut(s) 499, 593, 861, 1405
BtsIMutI CAGTG 2 cut(s) 553, 758
Cac8I GCNNGC 1 cut(s) 593
CciI TCATGA 1 cut(s) 1730
Cfr13I GGNCC 1 cut(s) 1551
ClaI ATCGAT 1 cut(s) 1149
CseI GACGC 1 cut(s) 152
Csp6I GTAC 1 cut(s) 1430
CviAII CATG 6 cut(s) 532, 1012, 1108, 1384, 1466, 1731
CviQI GTAC 1 cut(s) 1430
DdeI CTNAG 4 cut(s) 441, 1200, 1301, 1511
DpnI GATC 4 cut(s) 52, 990, 1230, 1701
DpnII GATC 4 cut(s) 50, 988, 1228, 1699
DraI TTTAAA 1 cut(s) 367
EaeI YGGCCR 3 cut(s) 231, 748, 1216
EagI CGGCCG 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 191, 674
EarI CTCTTC 2 cut(s) 191, 674
EclXI CGGCCG 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 1011
Eco24I GRGCYC 1 cut(s) 1010
Eco31I GGTCTC 1 cut(s) 266
Eco32I GATATC 1 cut(s) 916
Eco47I GGWCC 1 cut(s) 1551
Eco52I CGGCCG 1 cut(s) 231
EcoRV GATATC 1 cut(s) 916
EcoT14I CCWWGG 1 cut(s) 1011
EcoT22I ATGCAT 1 cut(s) 1654
EcoT38I GRGCYC 1 cut(s) 1010
ErhI CCWWGG 1 cut(s) 1011
FaeI CATG 6 cut(s) 535, 1015, 1111, 1387, 1469, 1734
FaqI GGGAC 1 cut(s) 1125
FatI CATG 6 cut(s) 531, 1011, 1107, 1383, 1465, 1730
Fnu4HI GCNGC 2 cut(s) 447, 552
FokI GGATG 4 cut(s) 506, 600, 868, 1412
FriOI GRGCYC 1 cut(s) 1010
Fsp4HI GCNGC 2 cut(s) 447, 552
FspBI CTAG 5 cut(s) 72, 86, 647, 1292, 1331
GluI GCNGC 2 cut(s) 447, 552
GsaI CCCAGC 1 cut(s) 1380
GsuI CTGGAG 2 cut(s) 410, 1524
HaeIII GGCC 5 cut(s) 233, 750, 1079, 1218, 1260
HapII CCGG 2 cut(s) 491, 1703
HgaI GACGC 1 cut(s) 152
Hin1II CATG 6 cut(s) 535, 1015, 1111, 1387, 1469, 1734
HinfI GANTC 9 cut(s) 202, 270, 325, 727, 735, 790, 1388, 1462, 1727
HpaII CCGG 2 cut(s) 491, 1703
HphI GGTGA 3 cut(s) 382, 799, 808
Hpy166II GTNNAC 3 cut(s) 501, 1345, 1679
Hpy188I TCNGA 4 cut(s) 269, 1026, 1201, 1571
Hpy188III TCNNGA 8 cut(s) 48, 199, 427, 806, 1094, 1226, 1541, 1731
Hpy8I GTNNAC 3 cut(s) 501, 1345, 1679
HpyAV CCTTC 5 cut(s) 406, 829, 997, 1307, 1355
HpyCH4III ACNGT 7 cut(s) 548, 629, 944, 950, 1055, 1129, 1683
HpyCH4IV ACGT 1 cut(s) 705
HpyCH4V TGCA 9 cut(s) 531, 551, 741, 839, 1207, 1371, 1394, 1646, 1652
HpyF10VI GCNNNNNNNGC 7 cut(s) 91, 160, 169, 281, 446, 747, 1601
HpyF3I CTNAG 4 cut(s) 441, 1200, 1301, 1511
HpySE526I ACGT 1 cut(s) 705
Hsp92II CATG 6 cut(s) 535, 1015, 1111, 1387, 1469, 1734
Kzo9I GATC 4 cut(s) 50, 988, 1228, 1699
LmnI GCTCC 2 cut(s) 703, 1013
Lsp1109I GCAGC 2 cut(s) 458, 563
LweI GCATC 6 cut(s) 578, 826, 1194, 1604, 1639, 1661
MaeI CTAG 5 cut(s) 72, 86, 647, 1292, 1331
MaeII ACGT 1 cut(s) 705
MaeIII GTNAC 7 cut(s) 388, 709, 814, 944, 1031, 1129, 1265
MalI GATC 4 cut(s) 52, 990, 1230, 1701
MboI GATC 4 cut(s) 50, 988, 1228, 1699
MboII GAAGA 6 cut(s) 178, 368, 583, 590, 661, 1396
MflI RGATCY 1 cut(s) 1699
MhlI GDGCHC 1 cut(s) 1010
MlsI TGGCCA 1 cut(s) 1218
MluCI AATT 9 cut(s) 215, 307, 359, 368, 560, 1220, 1236, 1295, 1664
MluNI TGGCCA 1 cut(s) 1218
MlyI GAGTC 3 cut(s) 196, 744, 799
MmeI TCCRAC 2 cut(s) 1305, 1529
Mox20I TGGCCA 1 cut(s) 1218
Mph1103I ATGCAT 1 cut(s) 1654
MroXI GAANNNNTTC 3 cut(s) 153, 274, 668
MscI TGGCCA 1 cut(s) 1218
MseI TTAA 6 cut(s) 306, 366, 1017, 1490, 1580, 1721
MslI CAYNNNNRTG 1 cut(s) 677
Msp20I TGGCCA 1 cut(s) 1218
MspI CCGG 2 cut(s) 491, 1703
Mva1269I GAATGC 1 cut(s) 133
MvnI CGCG 1 cut(s) 163
MwoI GCNNNNNNNGC 7 cut(s) 91, 160, 169, 281, 446, 747, 1601
NcoI CCATGG 1 cut(s) 1011
NdeII GATC 4 cut(s) 50, 988, 1228, 1699
NlaIII CATG 6 cut(s) 535, 1015, 1111, 1387, 1469, 1734
NlaIV GGNNCC 1 cut(s) 1009
NmuCI GTSAC 2 cut(s) 388, 814
NsiI ATGCAT 1 cut(s) 1654
PagI TCATGA 1 cut(s) 1730
PctI GAATGC 1 cut(s) 133
PdmI GAANNNNTTC 3 cut(s) 153, 274, 668
PfeI GAWTC 6 cut(s) 270, 325, 727, 1388, 1462, 1727
PkrI GCNGC 2 cut(s) 448, 553
PleI GAGTC 3 cut(s) 196, 743, 798
PpsI GAGTC 3 cut(s) 196, 743, 798
PshBI ATTAAT 1 cut(s) 306
PsiI TTATAA 2 cut(s) 1137, 1242
Psp1406I AACGTT 1 cut(s) 705
PspFI CCCAGC 1 cut(s) 1376
PspN4I GGNNCC 1 cut(s) 1009
PspPI GGNCC 1 cut(s) 1551
PstI CTGCAG 1 cut(s) 1648
PsuI RGATCY 1 cut(s) 1699
RsaI GTAC 1 cut(s) 1431
RsaNI GTAC 1 cut(s) 1430
RseI CAYNNNNRTG 1 cut(s) 677
SaqAI TTAA 6 cut(s) 306, 366, 1017, 1490, 1580, 1721
SatI GCNGC 2 cut(s) 447, 552
Sau3AI GATC 4 cut(s) 50, 988, 1228, 1699
Sau96I GGNCC 1 cut(s) 1551
SchI GAGTC 3 cut(s) 196, 744, 799
SduI GDGCHC 1 cut(s) 1010
SfaNI GCATC 6 cut(s) 578, 826, 1194, 1604, 1639, 1661
SfcI CTRYAG 3 cut(s) 610, 1284, 1644
SinI GGWCC 1 cut(s) 1551
SmiMI CAYNNNNRTG 1 cut(s) 677
SpeI ACTAGT 1 cut(s) 646
Sse9I AATT 9 cut(s) 215, 307, 359, 368, 560, 1220, 1236, 1295, 1664
SspI AATATT 3 cut(s) 298, 1246, 1351
SspMI CTAG 5 cut(s) 72, 86, 647, 1292, 1331
StyI CCWWGG 1 cut(s) 1011
TaaI ACNGT 7 cut(s) 548, 629, 944, 950, 1055, 1129, 1683
TaiI ACGT 1 cut(s) 708
TaqI TCGA 4 cut(s) 49, 144, 624, 1149
TasI AATT 9 cut(s) 215, 307, 359, 368, 560, 1220, 1236, 1295, 1664
TatI WGTACW 1 cut(s) 1429
TfiI GAWTC 6 cut(s) 270, 325, 727, 1388, 1462, 1727
Tru1I TTAA 6 cut(s) 306, 366, 1017, 1490, 1580, 1721
Tru9I TTAA 6 cut(s) 306, 366, 1017, 1490, 1580, 1721
TscAI CASTG 2 cut(s) 553, 758
TseFI GTSAC 2 cut(s) 388, 814
TseI GCWGC 2 cut(s) 446, 551
Tsp45I GTSAC 2 cut(s) 388, 814
TspDTI ATGAA 7 cut(s) 32, 162, 669, 795, 1454, 1622, 1719
TspGWI ACGGA 1 cut(s) 1062
TspRI CASTG 2 cut(s) 553, 758
VpaK11BI GGWCC 1 cut(s) 1551
VspI ATTAAT 1 cut(s) 306
XapI RAATTY 1 cut(s) 1236
XmnI GAANNNNTTC 3 cut(s) 153, 274, 668
XspI CTAG 5 cut(s) 72, 86, 647, 1292, 1331
Zsp2I ATGCAT 1 cut(s) 1654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.