MD05G1004400.v1.1

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008617
Physical Location & Seq
Reverse (-)
1310984 .. 1330137
19154 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1004400.v1.1.491

Sequence Viewer

Length: 999 bp
ATGCGCACCCTCCTCACTCTGAGGCCACCACCACCGTCGTCGTCGCTCCTCCTCCCATGCCTCTCCGCCCTCTCTCCTCCTCCCTCCTCTTCCTTCCTCTTCAGCTCCCGACCTAACAGGCGCTCCCACTTCCTCACCTCGTGCTCTTCTCTCAAGCAGTCCAAGAAGAAGTCCCTCCAGAACACCCCCAATGCTCCTCCGCAGAGCCAGCGCTGGTTCTTCAACACCCCCAAGAACGACGACGGCGGTGAAATTGAAAAGACCGGTGAGGCTGACAACGGCGCCTTCGACGGCGACACTGCGTTTAAAGGCACTGTTTTGGCCGGTGTTGTCCTGGTCGGTATTGTTGGTGGGTTCGCCGGTGTTGGGTATGTGTACAAGGATCAAATCAACGCCTTCTTGAATCAGTTTTCCACTTTCATTGAAGGTTATGGTCCAGCTGGATATGCTTTGTTTGTTGCAGTTTATGCCGGACTGGAAATCCTTGCGATTCCAGCAGTTCCATTGACCATGTCAGCTGGTCTTCTCTTTGGCACAGTTGTTGGTTCCATTCTCGTCTCTATAAGTGGCACGGTTGCTGCTAGTGTTGCCTTTTTGATTGCTAGATATTTTGCTCGTGAGCGTATTGTTAAAATGGTTGAGGGAAACAAAAAGTTTCTTGCCATTGATAAGGCTATTGGAGAAAACGGATTTAGAGTTGTCACTCTCCTTCGCTTGAGCCCTTTGCTTCCCTTTTCGTTGGGGAACTATTTATATGGATTGACATCTGTAAAGTTTGTACCATACGTGTTGGGAAGTTGGTTGGGGATGCTTCCAGGAACATGGGCTTACGTAAGTGCTGGTGCATTTGGGCGAGCTATAATTCAAGAAGAATCTGAAGTTGGTTTGCCTGGAGGAAATGGTCAGCTGTTGACACTAGGGCTTGGACTGTTAGCCACAGCATTGGCTGCTGCTTATGTAACACGACTAGCAAAGGATGCTGTAAAAGATATCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

333

Amino Acids

35.14

Weight (kDa)

9.33

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 164 - 282 2.1e-23 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 281
AciI CCGC 3 cut(s) 66, 200, 246
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 1 cut(s) 321
AcuI CTGAAG 2 cut(s) 85, 897
AcyI GRCGYC 1 cut(s) 282
AdeI CACNNNGTG 1 cut(s) 141
AfaI GTAC 2 cut(s) 377, 780
AfeI AGCGCT 1 cut(s) 212
AfiI CCNNNNNNNGG 1 cut(s) 366
AflIII ACRYGT 1 cut(s) 786
AgeI ACCGGT 1 cut(s) 263
AgsI TTSAA 5 cut(s) 223, 257, 403, 425, 866
AjnI CCWGG 3 cut(s) 333, 814, 889
AjuI GAANNNNNNNTTGG 2 cut(s) 864, 896
AluBI AGCT 5 cut(s) 105, 440, 518, 857, 907
AluI AGCT 5 cut(s) 105, 440, 518, 857, 907
Alw21I GWGCWC 1 cut(s) 146
Alw26I GTCTC 1 cut(s) 562
AlwI GGATC 1 cut(s) 390
Aor51HI AGCGCT 1 cut(s) 212
AoxI GGCC 2 cut(s) 23, 321
ApeKI GCWGC 3 cut(s) 578, 947, 950
AsiGI ACCGGT 1 cut(s) 263
AspLEI GCGC 4 cut(s) 6, 123, 213, 284
AspS9I GGNCC 1 cut(s) 434
AsuHPI GGTGA 3 cut(s) 127, 260, 278
AvaII GGWCC 1 cut(s) 434
BanI GGYRCC 1 cut(s) 281
BanII GRGCYC 1 cut(s) 722
BauI CACGAG 2 cut(s) 139, 615
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 3 cut(s) 565, 934, 937
BceAI ACGGC 3 cut(s) 259, 295, 307
BciT130I CCWGG 3 cut(s) 335, 816, 891
BcoDI GTCTC 1 cut(s) 562
BfaI CTAG 4 cut(s) 582, 603, 917, 968
BfoI RGCGCY 3 cut(s) 124, 214, 285
BisI GCNGC 3 cut(s) 579, 948, 951
BlsI GCNGC 3 cut(s) 580, 949, 952
Bme1390I CCNGG 3 cut(s) 335, 816, 891
Bme18I GGWCC 1 cut(s) 434
BmgT120I GGNCC 1 cut(s) 434
BmiI GGNNCC 2 cut(s) 283, 547
BmrFI CCNGG 3 cut(s) 335, 816, 891
BmsI GCATC 2 cut(s) 798, 967
BpiI GAAGAC 1 cut(s) 515
BpmI CTGGAG 2 cut(s) 161, 912
BpuEI CTTGAG 2 cut(s) 137, 736
Bsa29I ATCGAT 1 cut(s) 993
BsaAI YACGTR 2 cut(s) 787, 832
BsaBI GATNNNNATC 1 cut(s) 763
BsaHI GRCGYC 1 cut(s) 282
BsaWI WCCGGW 1 cut(s) 263
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse118I RCCGGY 3 cut(s) 263, 323, 359
Bse1I ACTGG 1 cut(s) 480
Bse8I GATNNNNATC 1 cut(s) 763
BseBI CCWGG 3 cut(s) 335, 816, 891
BseCI ATCGAT 1 cut(s) 993
BseGI GGATG 2 cut(s) 813, 982
BseJI GATNNNNATC 1 cut(s) 763
BseLI CCNNNNNNNGG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 11
BseNI ACTGG 1 cut(s) 480
BseRI GAGGAG 6 cut(s) 38, 41, 66, 69, 76, 186
BseXI GCAGC 3 cut(s) 565, 934, 937
BshFI GGCC 2 cut(s) 25, 323
BshNI GGYRCC 1 cut(s) 281
BshTI ACCGGT 1 cut(s) 263
BshVI ATCGAT 1 cut(s) 993
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 4 cut(s) 264, 324, 360, 471
BslFI GGGAC 1 cut(s) 157
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 1 cut(s) 562
BsmBI CGTCTC 1 cut(s) 562
BsmFI GGGAC 1 cut(s) 157
BsnI GGCC 2 cut(s) 25, 323
Bsp1286I GDGCHC 2 cut(s) 146, 722
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 1 cut(s) 382
BspACI CCGC 3 cut(s) 66, 200, 246
BspANI GGCC 2 cut(s) 25, 323
BspCNI CTCAG 1 cut(s) 12
BspDI ATCGAT 1 cut(s) 993
BspLI GGNNCC 2 cut(s) 283, 547
BspPI GGATC 1 cut(s) 390
BspQI GCTCTTC 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 281
BsrFI RCCGGY 3 cut(s) 263, 323, 359
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 1 cut(s) 480
BssAI RCCGGY 3 cut(s) 263, 323, 359
BssMI GATC 1 cut(s) 382
BssNI GRCGYC 1 cut(s) 282
BssSI CACGAG 2 cut(s) 139, 615
Bst2BI CACGAG 2 cut(s) 139, 615
Bst2UI CCWGG 3 cut(s) 335, 816, 891
Bst4CI ACNGT 5 cut(s) 36, 316, 538, 574, 930
Bst6I CTCTTC 3 cut(s) 94, 104, 151
BstACI GRCGYC 1 cut(s) 282
BstAPI GCANNNNNTGC 3 cut(s) 467, 947, 977
BstAUI TGTACA 1 cut(s) 375
BstBAI YACGTR 2 cut(s) 787, 832
BstC8I GCNNGC 2 cut(s) 209, 855
BstDEI CTNAG 1 cut(s) 20
BstF5I GGATG 2 cut(s) 813, 982
BstH2I RGCGCY 3 cut(s) 124, 214, 285
BstHHI GCGC 4 cut(s) 6, 123, 213, 284
BstKTI GATC 1 cut(s) 385
BstMAI GTCTC 1 cut(s) 562
BstMBI GATC 1 cut(s) 382
BstMWI GCNNNNNNNGC 7 cut(s) 208, 446, 467, 494, 587, 947, 977
BstNI CCWGG 3 cut(s) 335, 816, 891
BstSCI CCNGG 3 cut(s) 333, 814, 889
BstSNI TACGTA 1 cut(s) 832
BstV1I GCAGC 3 cut(s) 565, 934, 937
BstV2I GAAGAC 1 cut(s) 515
BstXI CCANNNNNNTGG 2 cut(s) 822, 943
Bsu15I ATCGAT 1 cut(s) 993
BsuRI GGCC 2 cut(s) 25, 323
BsuTUI ATCGAT 1 cut(s) 993
BtsCI GGATG 2 cut(s) 813, 982
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 2 cut(s) 297, 312
Cac8I GCNNGC 2 cut(s) 209, 855
CfoI GCGC 4 cut(s) 6, 123, 213, 284
Cfr10I RCCGGY 3 cut(s) 263, 323, 359
Cfr13I GGNCC 1 cut(s) 434
ClaI ATCGAT 1 cut(s) 993
Csp6I GTAC 2 cut(s) 376, 779
CspAI ACCGGT 1 cut(s) 263
CspCI CAANNNNNGTGG 2 cut(s) 403, 438
CviAII CATG 3 cut(s) 57, 511, 822
CviQI GTAC 2 cut(s) 376, 779
DdeI CTNAG 1 cut(s) 20
DinI GGCGCC 1 cut(s) 283
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
DraI TTTAAA 1 cut(s) 307
DraIII CACNNNGTG 1 cut(s) 141
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 3 cut(s) 94, 104, 151
EarI CTCTTC 3 cut(s) 94, 104, 151
EciI GGCGGA 1 cut(s) 55
Eco105I TACGTA 1 cut(s) 832
Eco24I GRGCYC 1 cut(s) 722
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 1 cut(s) 434
Eco47III AGCGCT 1 cut(s) 212
Eco57I CTGAAG 2 cut(s) 85, 897
EcoRII CCWGG 3 cut(s) 333, 814, 889
EcoRV GATATC 1 cut(s) 991
EcoT38I GRGCYC 1 cut(s) 722
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 562
FaeI CATG 3 cut(s) 60, 514, 825
FaqI GGGAC 1 cut(s) 157
FatI CATG 3 cut(s) 56, 510, 821
Fnu4HI GCNGC 3 cut(s) 579, 948, 951
FokI GGATG 2 cut(s) 820, 989
FriOI GRGCYC 1 cut(s) 722
Fsp4HI GCNGC 3 cut(s) 579, 948, 951
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 4 cut(s) 582, 603, 917, 968
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 4 cut(s) 5, 122, 212, 283
GluI GCNGC 3 cut(s) 579, 948, 951
GsuI CTGGAG 2 cut(s) 161, 912
HaeII RGCGCY 3 cut(s) 124, 214, 285
HaeIII GGCC 2 cut(s) 25, 323
HapII CCGG 4 cut(s) 264, 324, 360, 471
HhaI GCGC 4 cut(s) 6, 123, 213, 284
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 3 cut(s) 60, 514, 825
Hin6I GCGC 4 cut(s) 4, 121, 211, 282
HinP1I GCGC 4 cut(s) 4, 121, 211, 282
HincII GTYRAC 1 cut(s) 912
HindII GTYRAC 1 cut(s) 912
HinfI GANTC 3 cut(s) 403, 490, 872
HpaII CCGG 4 cut(s) 264, 324, 360, 471
HphI GGTGA 3 cut(s) 127, 260, 278
Hpy166II GTNNAC 2 cut(s) 376, 912
Hpy188I TCNGA 2 cut(s) 21, 877
Hpy188III TCNNGA 5 cut(s) 108, 178, 400, 617, 866
Hpy8I GTNNAC 2 cut(s) 376, 912
Hpy99I CGWCG 6 cut(s) 40, 43, 46, 242, 245, 293
HpyAV CCTTC 5 cut(s) 103, 295, 406, 419, 719
HpyCH4III ACNGT 5 cut(s) 36, 316, 538, 574, 930
HpyCH4IV ACGT 2 cut(s) 786, 831
HpyCH4V TGCA 2 cut(s) 461, 845
HpyF10VI GCNNNNNNNGC 7 cut(s) 208, 446, 467, 494, 587, 947, 977
HpyF3I CTNAG 1 cut(s) 20
HpySE526I ACGT 2 cut(s) 786, 831
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 3 cut(s) 60, 514, 825
HspAI GCGC 4 cut(s) 4, 121, 211, 282
KasI GGCGCC 1 cut(s) 281
Kzo9I GATC 1 cut(s) 382
LguI GCTCTTC 1 cut(s) 151
LmnI GCTCC 4 cut(s) 51, 110, 128, 199
Lsp1109I GCAGC 3 cut(s) 565, 934, 937
LweI GCATC 2 cut(s) 798, 967
MaeI CTAG 4 cut(s) 582, 603, 917, 968
MaeII ACGT 2 cut(s) 786, 831
MaeIII GTNAC 2 cut(s) 700, 958
MalI GATC 1 cut(s) 384
MboI GATC 1 cut(s) 382
MboII GAAGA 7 cut(s) 81, 91, 138, 178, 211, 515, 881
MhlI GDGCHC 2 cut(s) 146, 722
MluCI AATT 2 cut(s) 252, 861
Mly113I GGCGCC 1 cut(s) 282
MseI TTAA 2 cut(s) 306, 630
MspA1I CMGCKG 3 cut(s) 440, 518, 907
MspI CCGG 4 cut(s) 264, 324, 360, 471
MspR9I CCNGG 3 cut(s) 335, 816, 891
MvaI CCWGG 3 cut(s) 335, 816, 891
MwoI GCNNNNNNNGC 7 cut(s) 208, 446, 467, 494, 587, 947, 977
NarI GGCGCC 1 cut(s) 282
NdeII GATC 1 cut(s) 382
NlaIII CATG 3 cut(s) 60, 514, 825
NlaIV GGNNCC 2 cut(s) 283, 547
NmuCI GTSAC 1 cut(s) 700
NsbI TGCGCA 1 cut(s) 5
PciSI GCTCTTC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PfeI GAWTC 3 cut(s) 403, 490, 872
PflFI GACNNNGTC 1 cut(s) 511
PfoI TCCNGGA 1 cut(s) 814
PinAI ACCGGT 1 cut(s) 263
PkrI GCNGC 3 cut(s) 580, 949, 952
PluTI GGCGCC 1 cut(s) 285
Ppu21I YACGTR 2 cut(s) 787, 832
Psp6I CCWGG 3 cut(s) 333, 814, 889
PspGI CCWGG 3 cut(s) 333, 814, 889
PspN4I GGNNCC 2 cut(s) 283, 547
PspPI GGNCC 1 cut(s) 434
PsyI GACNNNGTC 1 cut(s) 511
PvuII CAGCTG 3 cut(s) 440, 518, 907
RsaI GTAC 2 cut(s) 377, 780
RsaNI GTAC 2 cut(s) 376, 779
SapI GCTCTTC 1 cut(s) 151
SaqAI TTAA 2 cut(s) 306, 630
SatI GCNGC 3 cut(s) 579, 948, 951
Sau3AI GATC 1 cut(s) 382
Sau96I GGNCC 1 cut(s) 434
ScrFI CCNGG 3 cut(s) 335, 816, 891
SduI GDGCHC 2 cut(s) 146, 722
SfaNI GCATC 2 cut(s) 798, 967
SfoI GGCGCC 1 cut(s) 283
SgrAI CRCCGGYG 1 cut(s) 359
SinI GGWCC 1 cut(s) 434
SmlI CTYRAG 2 cut(s) 152, 715
SmoI CTYRAG 2 cut(s) 152, 715
SnaBI TACGTA 1 cut(s) 832
Sse9I AATT 2 cut(s) 252, 861
SsiI CCGC 3 cut(s) 66, 200, 246
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 4 cut(s) 582, 603, 917, 968
StyD4I CCNGG 3 cut(s) 333, 814, 889
TaaI ACNGT 5 cut(s) 36, 316, 538, 574, 930
TaiI ACGT 2 cut(s) 789, 834
TaqI TCGA 2 cut(s) 288, 993
TasI AATT 2 cut(s) 252, 861
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 3 cut(s) 403, 490, 872
Tru1I TTAA 2 cut(s) 306, 630
Tru9I TTAA 2 cut(s) 306, 630
TscAI CASTG 2 cut(s) 304, 319
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 3 cut(s) 578, 947, 950
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 1 cut(s) 409
TspGWI ACGGA 1 cut(s) 702
TspRI CASTG 2 cut(s) 304, 319
Tth111I GACNNNGTC 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 434
XspI CTAG 4 cut(s) 582, 603, 917, 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.