Rw6G001140

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008617
Physical Location & Seq
Forward (+)
1673739 .. 1679347
5609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G001140.1

Sequence Viewer

Length: 1014 bp
ATGCGCACCCTCTTCCTCACTCTGAGGCCACCACCACCATCTCCGCCGTGCCTCTCTCCCTTCTCCTCCTCCTCCTCCCACCCTTCTTTCCTCTTCAAACCCAACCACCACCGCTTCCACTTCCTCTCGCCATGCTCCTCTCTCAAGCAGACCAAGAACAAGACCAGCCGCCTCCAGAAGACCACCACTGCTCCGCCGCAGAGCCTCAAGTGGCTCTTCAATAATCCCAAGAGTGACGACGGCGGCGAGATTGAAAAAATCGGCGGCGGCGGCGGCGGCGGCGGTGGTGGTGATGATGAGGGCGAGGGTGGGTTGGCGCTTAAAGGTACTGTCTTGGCGGGGCTGTTGTTGGTTGGGCTTGTTGGTGGGTTTGGGGCGGCTGGGTATGTGTACAAGGATCAGGTTAATGCCTTCTTGAATCAGTTTACGACTCTCATTGAAGGGTATGGTCCAGCGGGATATGCTTTGTTTGTTGCAGTTTATGCAGGACTGGAAATTCTTGCGATTCCAGCTGTTCCATTGACCATGTCAGCTGGTCTTCTTTTTGGCACAGTTACTGGATCAATTCTAGTCTCTCTAAGCGGAACGGTGGCTGCTAGCGTTGCTTTTCTGATTGCTAGATATTTTGCTCGTGAACGTATTCTTAAAATGGTTGAGGGAAACAAAAAGTTTCTTGCAATTGATAAGGCTATTGGAGAAAATGGCTTCAGAGTTGTTACTCTCCTCCGTTTGAGCCCTTTGCTTCCTTTTTCTCTGGGGAACTATTTATATGGATTGACATCTGTAAAGTTTGTCCCATATGTGTTGGGAAGTTGGTTGGGGATGCTTCCAGGAACATGGGCTTATGTGAGTGCTGGTGCATTTGGGCGAGCCATAATTCAAGAGGAATCTGATGTTGGTTTGCCCGGAGGAAATGGTCAGCTGTTGACACTAGCAGCTGGACTGTTAGTCACAGCATTAGCTGCTGCTTATGTAACACGATTAGCAAAGGATGCTGTAAAAGATATCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

337

Amino Acids

35.33

Weight (kDa)

9.3

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 169 - 287 1.8e-23 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AclWI GGATC 2 cut(s) 405, 568
AcsI RAATTY 1 cut(s) 495
AcuI CTGAAG 1 cut(s) 691
AfaI GTAC 2 cut(s) 328, 392
AgsI TTSAA 6 cut(s) 97, 220, 254, 418, 440, 881
AhdI GACNNNNNGTC 1 cut(s) 947
AjnI CCWGG 1 cut(s) 829
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AluBI AGCT 5 cut(s) 512, 533, 922, 938, 962
AluI AGCT 5 cut(s) 512, 533, 922, 938, 962
Alw26I GTCTC 1 cut(s) 577
AlwI GGATC 2 cut(s) 405, 568
AlwNI CAGNNNCTG 1 cut(s) 557
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 4 cut(s) 593, 935, 962, 965
ApoI RAATTY 1 cut(s) 495
Asp700I GAANNNNTTC 1 cut(s) 639
AspLEI GCGC 2 cut(s) 6, 319
AspS9I GGNCC 1 cut(s) 449
AsuC2I CCSGG 1 cut(s) 906
AsuHPI GGTGA 1 cut(s) 302
AsuNHI GCTAGC 1 cut(s) 596
AvaII GGWCC 1 cut(s) 449
BanII GRGCYC 1 cut(s) 737
BauI CACGAG 1 cut(s) 630
BbsI GAAGAC 2 cut(s) 185, 530
BbvI GCAGC 4 cut(s) 580, 947, 949, 952
BccI CCATC 1 cut(s) 46
BceAI ACGGC 2 cut(s) 31, 256
BciT130I CCWGG 1 cut(s) 831
BcnI CCSGG 1 cut(s) 906
BcoDI GTCTC 1 cut(s) 577
BfaI CTAG 4 cut(s) 569, 597, 618, 932
BfoI RGCGCY 1 cut(s) 320
Bme1390I CCNGG 2 cut(s) 831, 906
Bme18I GGWCC 1 cut(s) 449
BmeRI GACNNNNNGTC 1 cut(s) 947
BmgT120I GGNCC 1 cut(s) 449
BmrFI CCNGG 2 cut(s) 831, 906
BmsI GCATC 2 cut(s) 813, 982
BmtI GCTAGC 1 cut(s) 600
BpiI GAAGAC 2 cut(s) 185, 530
BpmI CTGGAG 1 cut(s) 158
BpuEI CTTGAG 2 cut(s) 128, 191
BpuMI CCSGG 1 cut(s) 906
Bsa29I ATCGAT 1 cut(s) 1008
BsaBI GATNNNNATC 1 cut(s) 778
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bse1I ACTGG 2 cut(s) 495, 562
Bse8I GATNNNNATC 1 cut(s) 778
BseBI CCWGG 1 cut(s) 831
BseCI ATCGAT 1 cut(s) 1008
BseGI GGATG 2 cut(s) 828, 997
BseJI GATNNNNATC 1 cut(s) 778
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 2 cut(s) 495, 562
BseRI GAGGAG 6 cut(s) 55, 58, 61, 64, 127, 713
BseXI GCAGC 4 cut(s) 580, 947, 949, 952
BseYI CCCAGC 1 cut(s) 380
BshFI GGCC 1 cut(s) 28
BshVI ATCGAT 1 cut(s) 1008
BsiSI CCGG 1 cut(s) 906
BslFI GGGAC 1 cut(s) 779
BsmAI GTCTC 1 cut(s) 577
BsmFI GGGAC 1 cut(s) 779
BsnI GGCC 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 737
Bsp1407I TGTACA 1 cut(s) 390
Bsp143I GATC 2 cut(s) 397, 560
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 1 cut(s) 15
BspDI ATCGAT 1 cut(s) 1008
BspOI GCTAGC 1 cut(s) 600
BspPI GGATC 2 cut(s) 405, 568
BspQI GCTCTTC 1 cut(s) 221
BsrGI TGTACA 1 cut(s) 390
BsrI ACTGG 2 cut(s) 495, 562
BssMI GATC 2 cut(s) 397, 560
BssSI CACGAG 1 cut(s) 630
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 1 cut(s) 831
Bst4CI ACNGT 4 cut(s) 331, 553, 589, 945
Bst6I CTCTTC 3 cut(s) 17, 98, 221
BstAPI GCANNNNNTGC 3 cut(s) 482, 962, 992
BstAUI TGTACA 1 cut(s) 390
BstC8I GCNNGC 2 cut(s) 598, 870
BstDEI CTNAG 2 cut(s) 23, 578
BstF5I GGATG 2 cut(s) 828, 997
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 2 cut(s) 6, 319
BstKTI GATC 2 cut(s) 400, 563
BstMAI GTCTC 1 cut(s) 577
BstMBI GATC 2 cut(s) 397, 560
BstNI CCWGG 1 cut(s) 831
BstSCI CCNGG 2 cut(s) 829, 904
BstV1I GCAGC 4 cut(s) 580, 947, 949, 952
BstV2I GAAGAC 2 cut(s) 185, 530
BstXI CCANNNNNNTGG 1 cut(s) 837
Bsu15I ATCGAT 1 cut(s) 1008
BsuRI GGCC 1 cut(s) 28
BsuTUI ATCGAT 1 cut(s) 1008
BtsCI GGATG 2 cut(s) 828, 997
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 2 cut(s) 598, 870
CaiI CAGNNNCTG 1 cut(s) 557
CfoI GCGC 2 cut(s) 6, 319
Cfr13I GGNCC 1 cut(s) 449
ClaI ATCGAT 1 cut(s) 1008
Csp6I GTAC 2 cut(s) 327, 391
CviAII CATG 3 cut(s) 132, 526, 837
CviQI GTAC 2 cut(s) 327, 391
DdeI CTNAG 2 cut(s) 23, 578
DpnI GATC 2 cut(s) 399, 562
DpnII GATC 2 cut(s) 397, 560
DriI GACNNNNNGTC 1 cut(s) 947
Eam1104I CTCTTC 3 cut(s) 17, 98, 221
Eam1105I GACNNNNNGTC 1 cut(s) 947
EarI CTCTTC 3 cut(s) 17, 98, 221
EciI GGCGGA 2 cut(s) 33, 183
Eco24I GRGCYC 1 cut(s) 737
Eco32I GATATC 1 cut(s) 1006
Eco47I GGWCC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 691
EcoRII CCWGG 1 cut(s) 829
EcoRV GATATC 1 cut(s) 1006
EcoT38I GRGCYC 1 cut(s) 737
FaeI CATG 3 cut(s) 135, 529, 840
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 779
FatI CATG 3 cut(s) 131, 525, 836
FauI CCCGC 2 cut(s) 331, 448
FauNDI CATATG 1 cut(s) 799
FokI GGATG 2 cut(s) 835, 1004
FriOI GRGCYC 1 cut(s) 737
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 4 cut(s) 569, 597, 618, 932
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 2 cut(s) 5, 318
GsaI CCCAGC 1 cut(s) 384
GsuI CTGGAG 1 cut(s) 158
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 1 cut(s) 28
HapII CCGG 1 cut(s) 906
HhaI GCGC 2 cut(s) 6, 319
Hin1II CATG 3 cut(s) 135, 529, 840
Hin6I GCGC 2 cut(s) 4, 317
HinP1I GCGC 2 cut(s) 4, 317
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HinfI GANTC 4 cut(s) 418, 430, 505, 887
HpaII CCGG 1 cut(s) 906
HphI GGTGA 1 cut(s) 302
Hpy166II GTNNAC 4 cut(s) 391, 426, 635, 927
Hpy188I TCNGA 4 cut(s) 24, 612, 710, 892
Hpy188III TCNNGA 4 cut(s) 175, 415, 632, 881
Hpy8I GTNNAC 4 cut(s) 391, 426, 635, 927
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 4 cut(s) 70, 93, 421, 434
HpyCH4III ACNGT 4 cut(s) 331, 553, 589, 945
HpyCH4IV ACGT 1 cut(s) 637
HpyCH4V TGCA 4 cut(s) 476, 485, 677, 860
HpyF3I CTNAG 2 cut(s) 23, 578
HpySE526I ACGT 1 cut(s) 637
Hsp92II CATG 3 cut(s) 135, 529, 840
HspAI GCGC 2 cut(s) 4, 317
Kzo9I GATC 2 cut(s) 397, 560
LguI GCTCTTC 1 cut(s) 221
LmnI GCTCC 2 cut(s) 140, 196
Lsp1109I GCAGC 4 cut(s) 580, 947, 949, 952
LweI GCATC 2 cut(s) 813, 982
MaeI CTAG 4 cut(s) 569, 597, 618, 932
MaeII ACGT 1 cut(s) 637
MaeIII GTNAC 5 cut(s) 233, 553, 715, 949, 973
MalI GATC 2 cut(s) 399, 562
MboI GATC 2 cut(s) 397, 560
MboII GAAGA 5 cut(s) 4, 85, 190, 208, 530
MfeI CAATTG 1 cut(s) 678
MhlI GDGCHC 1 cut(s) 737
MluCI AATT 4 cut(s) 495, 564, 678, 876
MlyI GAGTC 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 639
MseI TTAA 3 cut(s) 321, 405, 645
MspA1I CMGCKG 5 cut(s) 455, 512, 533, 922, 938
MspI CCGG 1 cut(s) 906
MspR9I CCNGG 2 cut(s) 831, 906
MunI CAATTG 1 cut(s) 678
MvaI CCWGG 1 cut(s) 831
NciI CCSGG 1 cut(s) 906
NdeI CATATG 1 cut(s) 799
NdeII GATC 2 cut(s) 397, 560
NheI GCTAGC 1 cut(s) 596
NlaIII CATG 3 cut(s) 135, 529, 840
NmuCI GTSAC 2 cut(s) 233, 949
NsbI TGCGCA 1 cut(s) 5
PciSI GCTCTTC 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PdmI GAANNNNTTC 1 cut(s) 639
PfeI GAWTC 3 cut(s) 418, 505, 887
PflFI GACNNNGTC 1 cut(s) 526
PfoI TCCNGGA 1 cut(s) 829
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
Psp6I CCWGG 1 cut(s) 829
PspFI CCCAGC 1 cut(s) 380
PspGI CCWGG 1 cut(s) 829
PspPI GGNCC 1 cut(s) 449
PstNI CAGNNNCTG 1 cut(s) 557
PsyI GACNNNGTC 1 cut(s) 526
PvuII CAGCTG 4 cut(s) 512, 533, 922, 938
RsaI GTAC 2 cut(s) 328, 392
RsaNI GTAC 2 cut(s) 327, 391
SapI GCTCTTC 1 cut(s) 221
SaqAI TTAA 3 cut(s) 321, 405, 645
Sau3AI GATC 2 cut(s) 397, 560
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 2 cut(s) 831, 906
SduI GDGCHC 1 cut(s) 737
SetI ASST 8 cut(s) 328, 405, 514, 535, 640, 924, 940, 964
SfaNI GCATC 2 cut(s) 813, 982
SinI GGWCC 1 cut(s) 449
SmlI CTYRAG 2 cut(s) 143, 206
SmoI CTYRAG 2 cut(s) 143, 206
Sse9I AATT 4 cut(s) 495, 564, 678, 876
SspMI CTAG 4 cut(s) 569, 597, 618, 932
StyD4I CCNGG 2 cut(s) 829, 904
TaaI ACNGT 4 cut(s) 331, 553, 589, 945
TaiI ACGT 1 cut(s) 640
TaqI TCGA 1 cut(s) 1008
TasI AATT 4 cut(s) 495, 564, 678, 876
TatI WGTACW 1 cut(s) 390
TfiI GAWTC 3 cut(s) 418, 505, 887
Tru1I TTAA 3 cut(s) 321, 405, 645
Tru9I TTAA 3 cut(s) 321, 405, 645
TscAI CASTG 1 cut(s) 193
TseFI GTSAC 2 cut(s) 233, 949
TseI GCWGC 4 cut(s) 593, 935, 962, 965
Tsp45I GTSAC 2 cut(s) 233, 949
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 1 cut(s) 193
Tth111I GACNNNGTC 1 cut(s) 526
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 1 cut(s) 495
XmnI GAANNNNTTC 1 cut(s) 639
XspI CTAG 4 cut(s) 569, 597, 618, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.