MD10G1004900.v1.1

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008617
Physical Location & Seq
Forward (+)
792571 .. 807009
14439 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1004900.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGCGCACCCTCCTCACTCTTAGGCCACCACCGGTACCACCCTCTTCGTCGTCTCTTCTCCTTCCATGCCTCTCCGCGCTCTCTCCTCCTCCCTCCTCCTCCCACCTTTTCAGCTTCCGAACCAACAAGCGCTTCCACTTCCTCACCCCGTGCTCCTCTCTCAAGCAGTCCAAGAAGAAGTCCCTCCAGAAGACCCCTAATGCTCCTCCGCAGAGCCAGCGCTGGTTCTTCAACACCCCCAAGAGCGATGATGGCGGCGATATTGAAAAGACAGACGAGGATGAAGACGGTGGTTTCGACGGCGACAGTGCGCTTAAAGGCACCGTTTTGGCCGGTGTTTTCCTGGTTGGTATTGTAAGTGGGTTCGCTGGTGTTGGGTACGTCTACAAGGACCAGATCAACGCCTTCTTGAATCAGTTTTCCACTCTCATTGAAGGTTATGGTCCAGCTGGATATGCTTTGTTTGTAGCAGTTTATGCCGGACTGGAAATCATTGCGATTCCAGCTATTCCATTGACCATGTCAGCTGGTCTTCTCTTTGGCACAGTTACTGGTTCTATTCTTGTCTCTATAAGTGGCACGGTGGCTGCTAGCATTGCCTTTTTGATTGCTAGATATTTCGCTCGTGAGCGTATTCTGAAAATGGTTGAGGGAAACAAGAAGTTTCTTGCCATTGATAAGGCTATTGGAGAAAATGGATTTAGAGTTGTCACTCTCCTTCGCTTGAGCCCTTTGCTTCCTTTTTCGTTGGGGAACTATTTGTATGGATTGACATCTGTAAAGTTTGTACCATACGTGTTAGGAAGTTGGTTGGGGATGCTTCCAGGAACATGGGCTTACGTGAGTGCTGGTGCATTTGGGCGAGCTATAATTCAAGAAGAATCTGAGGTTGGTTTGCCAGGAGGAAATGGTCAGCTCTTGACACTAGGGCTTGGACTGTTAGCCACAGCATTGGCTGCTGCTTATGTAACACGACTGGCAAAGGATGCTGTAAAAGATATCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

336

Amino Acids

35.63

Weight (kDa)

9.07

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 167 - 285 3.4e-23 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
Acc65I GGTACC 1 cut(s) 34
AccB1I GGYRCC 2 cut(s) 34, 320
AccI GTMKAC 1 cut(s) 384
AccII CGCG 1 cut(s) 77
AciI CCGC 3 cut(s) 75, 209, 255
AcoI YGGCCR 1 cut(s) 330
AdeI CACNNNGTG 1 cut(s) 150
AfaI GTAC 3 cut(s) 36, 380, 789
AfeI AGCGCT 2 cut(s) 131, 221
AflIII ACRYGT 1 cut(s) 795
AgeI ACCGGT 1 cut(s) 31
AgsI TTSAA 5 cut(s) 232, 266, 412, 434, 875
AjnI CCWGG 3 cut(s) 342, 823, 898
AjuI GAANNNNNNNTTGG 4 cut(s) 116, 148, 873, 905
AluBI AGCT 6 cut(s) 114, 449, 506, 527, 866, 916
AluI AGCT 6 cut(s) 114, 449, 506, 527, 866, 916
Alw21I GWGCWC 1 cut(s) 155
Alw26I GTCTC 2 cut(s) 57, 571
AlwNI CAGNNNCTG 1 cut(s) 551
Aor51HI AGCGCT 2 cut(s) 131, 221
AoxI GGCC 2 cut(s) 23, 330
ApeKI GCWGC 3 cut(s) 587, 956, 959
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AsiGI ACCGGT 1 cut(s) 31
Asp718I GGTACC 1 cut(s) 34
AspLEI GCGC 5 cut(s) 6, 79, 132, 222, 313
AspS9I GGNCC 2 cut(s) 391, 443
AsuHPI GGTGA 1 cut(s) 136
AsuNHI GCTAGC 1 cut(s) 590
AvaII GGWCC 2 cut(s) 391, 443
BanI GGYRCC 2 cut(s) 34, 320
BanII GRGCYC 1 cut(s) 731
BauI CACGAG 1 cut(s) 624
BbsI GAAGAC 3 cut(s) 197, 291, 524
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 3 cut(s) 574, 943, 946
BccI CCATC 1 cut(s) 245
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 3 cut(s) 344, 825, 900
BcoDI GTCTC 2 cut(s) 57, 571
BfaI CTAG 3 cut(s) 591, 612, 926
BfoI RGCGCY 2 cut(s) 133, 223
BisI GCNGC 4 cut(s) 256, 588, 957, 960
BlsI GCNGC 4 cut(s) 257, 589, 958, 961
Bme1390I CCNGG 3 cut(s) 344, 825, 900
Bme18I GGWCC 2 cut(s) 391, 443
BmgT120I GGNCC 2 cut(s) 391, 443
BmiI GGNNCC 2 cut(s) 36, 322
BmrFI CCNGG 3 cut(s) 344, 825, 900
BmsI GCATC 2 cut(s) 807, 976
BmtI GCTAGC 1 cut(s) 594
BpiI GAAGAC 3 cut(s) 197, 291, 524
BpmI CTGGAG 1 cut(s) 170
BpuEI CTTGAG 2 cut(s) 146, 745
Bsa29I ATCGAT 1 cut(s) 1002
BsaAI YACGTR 2 cut(s) 796, 841
BsaBI GATNNNNATC 1 cut(s) 772
BsaWI WCCGGW 1 cut(s) 31
Bse118I RCCGGY 2 cut(s) 31, 332
Bse1I ACTGG 3 cut(s) 489, 556, 981
Bse3DI GCAATG 2 cut(s) 492, 594
Bse8I GATNNNNATC 1 cut(s) 772
BseBI CCWGG 3 cut(s) 344, 825, 900
BseCI ATCGAT 1 cut(s) 1002
BseGI GGATG 3 cut(s) 286, 822, 991
BseJI GATNNNNATC 1 cut(s) 772
BseMI GCAATG 2 cut(s) 492, 594
BseMII CTCAG 1 cut(s) 876
BseNI ACTGG 3 cut(s) 489, 556, 981
BseRI GAGGAG 6 cut(s) 75, 78, 85, 88, 145, 195
BseXI GCAGC 3 cut(s) 574, 943, 946
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 2 cut(s) 25, 332
BshNI GGYRCC 2 cut(s) 34, 320
BshTI ACCGGT 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 1002
BsiHKAI GWGCWC 1 cut(s) 155
BsiSI CCGG 3 cut(s) 32, 333, 480
BslFI GGGAC 1 cut(s) 166
BsmAI GTCTC 2 cut(s) 57, 571
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 2 cut(s) 25, 332
Bsp1286I GDGCHC 2 cut(s) 155, 731
Bsp143I GATC 1 cut(s) 396
BspACI CCGC 3 cut(s) 75, 209, 255
BspANI GGCC 2 cut(s) 25, 332
BspCNI CTCAG 1 cut(s) 877
BspDI ATCGAT 1 cut(s) 1002
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 2 cut(s) 36, 322
BspOI GCTAGC 1 cut(s) 594
BspT107I GGYRCC 2 cut(s) 34, 320
BsrDI GCAATG 2 cut(s) 492, 594
BsrFI RCCGGY 2 cut(s) 31, 332
BsrI ACTGG 3 cut(s) 489, 556, 981
BssAI RCCGGY 2 cut(s) 31, 332
BssMI GATC 1 cut(s) 396
BssSI CACGAG 1 cut(s) 624
Bst2BI CACGAG 1 cut(s) 624
Bst2UI CCWGG 3 cut(s) 344, 825, 900
Bst4CI ACNGT 6 cut(s) 290, 308, 325, 547, 583, 939
Bst6I CTCTTC 2 cut(s) 49, 60
BstAPI GCANNNNNTGC 3 cut(s) 476, 956, 986
BstBAI YACGTR 2 cut(s) 796, 841
BstC8I GCNNGC 3 cut(s) 218, 592, 864
BstDEI CTNAG 2 cut(s) 20, 885
BstF5I GGATG 3 cut(s) 286, 822, 991
BstFNI CGCG 1 cut(s) 77
BstH2I RGCGCY 2 cut(s) 133, 223
BstHHI GCGC 5 cut(s) 6, 79, 132, 222, 313
BstKTI GATC 1 cut(s) 399
BstMAI GTCTC 2 cut(s) 57, 571
BstMBI GATC 1 cut(s) 396
BstMWI GCNNNNNNNGC 8 cut(s) 217, 252, 455, 476, 503, 596, 956, 986
BstNI CCWGG 3 cut(s) 344, 825, 900
BstSCI CCNGG 3 cut(s) 342, 823, 898
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 3 cut(s) 574, 943, 946
BstV2I GAAGAC 3 cut(s) 197, 291, 524
BstXI CCANNNNNNTGG 2 cut(s) 831, 952
Bsu15I ATCGAT 1 cut(s) 1002
BsuRI GGCC 2 cut(s) 25, 332
BsuTUI ATCGAT 1 cut(s) 1002
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 3 cut(s) 286, 822, 991
BtsIMutI CAGTG 1 cut(s) 313
Cac8I GCNNGC 3 cut(s) 218, 592, 864
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 5 cut(s) 6, 79, 132, 222, 313
Cfr10I RCCGGY 2 cut(s) 31, 332
Cfr13I GGNCC 2 cut(s) 391, 443
ClaI ATCGAT 1 cut(s) 1002
Csp6I GTAC 3 cut(s) 35, 379, 788
CspAI ACCGGT 1 cut(s) 31
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 3 cut(s) 66, 520, 831
CviQI GTAC 3 cut(s) 35, 379, 788
DdeI CTNAG 2 cut(s) 20, 885
DpnI GATC 1 cut(s) 398
DpnII GATC 1 cut(s) 396
DraIII CACNNNGTG 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 330
Eam1104I CTCTTC 2 cut(s) 49, 60
EarI CTCTTC 2 cut(s) 49, 60
Eco24I GRGCYC 1 cut(s) 731
Eco32I GATATC 1 cut(s) 1000
Eco47I GGWCC 2 cut(s) 391, 443
Eco47III AGCGCT 2 cut(s) 131, 221
EcoRII CCWGG 3 cut(s) 342, 823, 898
EcoRV GATATC 1 cut(s) 1000
EcoT38I GRGCYC 1 cut(s) 731
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 3 cut(s) 69, 523, 834
FaqI GGGAC 1 cut(s) 166
FatI CATG 3 cut(s) 65, 519, 830
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 4 cut(s) 256, 588, 957, 960
FokI GGATG 3 cut(s) 293, 829, 998
FriOI GRGCYC 1 cut(s) 731
Fsp4HI GCNGC 4 cut(s) 256, 588, 957, 960
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 3 cut(s) 591, 612, 926
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 5 cut(s) 5, 78, 131, 221, 312
GluI GCNGC 4 cut(s) 256, 588, 957, 960
GsuI CTGGAG 1 cut(s) 170
HaeII RGCGCY 2 cut(s) 133, 223
HaeIII GGCC 2 cut(s) 25, 332
HapII CCGG 3 cut(s) 32, 333, 480
HhaI GCGC 5 cut(s) 6, 79, 132, 222, 313
Hin1II CATG 3 cut(s) 69, 523, 834
Hin6I GCGC 5 cut(s) 4, 77, 130, 220, 311
HinP1I GCGC 5 cut(s) 4, 77, 130, 220, 311
HinfI GANTC 3 cut(s) 412, 499, 881
HpaII CCGG 3 cut(s) 32, 333, 480
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 3 cut(s) 119, 639, 886
Hpy188III TCNNGA 5 cut(s) 187, 409, 626, 875, 919
Hpy8I GTNNAC 1 cut(s) 385
Hpy99I CGWCG 2 cut(s) 52, 302
HpyAV CCTTC 4 cut(s) 71, 415, 428, 728
HpyCH4III ACNGT 6 cut(s) 290, 308, 325, 547, 583, 939
HpyCH4IV ACGT 3 cut(s) 381, 795, 840
HpyCH4V TGCA 1 cut(s) 854
HpyF10VI GCNNNNNNNGC 8 cut(s) 217, 252, 455, 476, 503, 596, 956, 986
HpyF3I CTNAG 2 cut(s) 20, 885
HpySE526I ACGT 3 cut(s) 381, 795, 840
Hsp92II CATG 3 cut(s) 69, 523, 834
HspAI GCGC 5 cut(s) 4, 77, 130, 220, 311
KpnI GGTACC 1 cut(s) 38
Kzo9I GATC 1 cut(s) 396
LmnI GCTCC 2 cut(s) 158, 208
Lsp1109I GCAGC 3 cut(s) 574, 943, 946
LweI GCATC 2 cut(s) 807, 976
MaeI CTAG 3 cut(s) 591, 612, 926
MaeII ACGT 3 cut(s) 381, 795, 840
MaeIII GTNAC 3 cut(s) 547, 709, 967
MalI GATC 1 cut(s) 398
MboI GATC 1 cut(s) 396
MboII GAAGA 8 cut(s) 36, 47, 187, 202, 220, 296, 524, 890
MhlI GDGCHC 2 cut(s) 155, 731
MluCI AATT 1 cut(s) 870
MseI TTAA 1 cut(s) 315
MspA1I CMGCKG 2 cut(s) 449, 527
MspI CCGG 3 cut(s) 32, 333, 480
MspR9I CCNGG 3 cut(s) 344, 825, 900
MvaI CCWGG 3 cut(s) 344, 825, 900
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 8 cut(s) 217, 252, 455, 476, 503, 596, 956, 986
NdeII GATC 1 cut(s) 396
NheI GCTAGC 1 cut(s) 590
NlaIII CATG 3 cut(s) 69, 523, 834
NlaIV GGNNCC 2 cut(s) 36, 322
NmuCI GTSAC 1 cut(s) 709
NsbI TGCGCA 1 cut(s) 5
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PfeI GAWTC 3 cut(s) 412, 499, 881
PflFI GACNNNGTC 1 cut(s) 520
PfoI TCCNGGA 1 cut(s) 823
PinAI ACCGGT 1 cut(s) 31
PkrI GCNGC 4 cut(s) 257, 589, 958, 961
Ppu21I YACGTR 2 cut(s) 796, 841
Psp6I CCWGG 3 cut(s) 342, 823, 898
PspGI CCWGG 3 cut(s) 342, 823, 898
PspN4I GGNNCC 2 cut(s) 36, 322
PspPI GGNCC 2 cut(s) 391, 443
PstNI CAGNNNCTG 1 cut(s) 551
PsyI GACNNNGTC 1 cut(s) 520
PvuII CAGCTG 2 cut(s) 449, 527
RsaI GTAC 3 cut(s) 36, 380, 789
RsaNI GTAC 3 cut(s) 35, 379, 788
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 4 cut(s) 256, 588, 957, 960
Sau3AI GATC 1 cut(s) 396
Sau96I GGNCC 2 cut(s) 391, 443
ScrFI CCNGG 3 cut(s) 344, 825, 900
SduI GDGCHC 2 cut(s) 155, 731
SfaNI GCATC 2 cut(s) 807, 976
SinI GGWCC 2 cut(s) 391, 443
SmlI CTYRAG 2 cut(s) 161, 724
SmoI CTYRAG 2 cut(s) 161, 724
Sse9I AATT 1 cut(s) 870
SsiI CCGC 3 cut(s) 75, 209, 255
SspMI CTAG 3 cut(s) 591, 612, 926
StyD4I CCNGG 3 cut(s) 342, 823, 898
TaaI ACNGT 6 cut(s) 290, 308, 325, 547, 583, 939
TaiI ACGT 3 cut(s) 384, 798, 843
TaqI TCGA 2 cut(s) 297, 1002
TasI AATT 1 cut(s) 870
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 3 cut(s) 412, 499, 881
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 3 cut(s) 587, 956, 959
Tsp45I GTSAC 1 cut(s) 709
TspDTI ATGAA 1 cut(s) 297
TspRI CASTG 1 cut(s) 313
Tth111I GACNNNGTC 1 cut(s) 520
VpaK11BI GGWCC 2 cut(s) 391, 443
XmiI GTMKAC 1 cut(s) 384
XspI CTAG 3 cut(s) 591, 612, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.