RLG00000015469

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008617
Physical Location & Seq
Reverse (-)
66531826 .. 66536785
4960 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015469

Sequence Viewer

Length: 1011 bp
ATGCGCACCCTCTTCCTCACTCTGAGGCCACCACCACCATCTCCGCCGTGCCTCTCTCCCTTCTCCTCCTCCTCCTCCTCCCACCCTTCTTTCCTCTTCAAACCCAACCACCACCGCTTCCACTTCCTCTCGCCATGCTCCTCTCTCAAGCAGACCAAGAACAAGACCAGCCGCCTCCAGAAGACCGCCACTGCTCCGCCGCAGAGCCTCAAGTGGCTCTTCAATAATCCCAAGAGTGACGACGGCGGCGAGATTGAAAAAATCGGCGGCGGCGCCGGCGGCGGTGGTGATGATGAGGGCGAGGGTGGGTTGGCGCTTAAAGGTACTGTCTTGGCGGGGCTGCTCTTGGTTGGGCTTGTTGGTGGGGTTGGGGCGGCTGGGTATGTGTACAAGGATCAGGTTAATGCCTTCTTGAATCAGTTCACGACTCTCATTGAAGGGTATGGTCCAGCGGGATATGCTTTGTTTGTAGCAGTGTATGCAGGACTGGAAATTCTTGCGATTCCAGCTGTTCCATTGACCATGTCAGCTGGTCTTCTTTTTGGCACAGTTACTGGATCAATTCTAGTCTCTCTAAGCGGAACGGTGGCTGCTAGCGTTGCTTTTCTGATTGCTAGATATTTTGCTCGTGAGCGTATTCTTAAAATGGTTGAGGGAAACAAAAAGTTTCTTGCAATTGATAAGGCTATTGGAGAAAATGGCTTCAGAGTTGTTACTCTCCTCCGTTTGAGCCCTTTGCTTCCTTTTTCTCTGGGGAACTATTTATATGGATTGACATCTGTAAAGTTTGTCCCATATGTGTTGGGAAGTTGGTTGGGGATGCTTCCAGGAACATGGGCTTATGTGAGTGCTGGTGCATTTGGGCGAGCCATAATTCAAGAGGAATCTGATGTTGGTTTGCCCGGAGGAAATGGTCAGCTGTTGACACTAGCAGCTGGACTGTTAGTCACAGCATTAGCTGCTGCTTATGTAACACGATTGGCAAAGGATGCTGTAAAAGATATCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

337

Amino Acids

35.24

Weight (kDa)

9.3

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 168 - 286 1.8e-23 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 272
AclWI GGATC 2 cut(s) 402, 565
AcsI RAATTY 1 cut(s) 492
AcuI CTGAAG 1 cut(s) 688
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 2 cut(s) 325, 389
AgsI TTSAA 6 cut(s) 100, 223, 257, 415, 437, 878
AhdI GACNNNNNGTC 1 cut(s) 944
AjnI CCWGG 1 cut(s) 826
AjuI GAANNNNNNNTTGG 2 cut(s) 876, 908
AluBI AGCT 5 cut(s) 509, 530, 919, 935, 959
AluI AGCT 5 cut(s) 509, 530, 919, 935, 959
Alw26I GTCTC 1 cut(s) 574
AlwI GGATC 2 cut(s) 402, 565
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 5 cut(s) 340, 590, 932, 959, 962
ApoI RAATTY 1 cut(s) 492
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 3 cut(s) 6, 275, 316
AspS9I GGNCC 1 cut(s) 446
AsuC2I CCSGG 1 cut(s) 903
AsuHPI GGTGA 1 cut(s) 299
AsuNHI GCTAGC 1 cut(s) 593
AvaII GGWCC 1 cut(s) 446
BanI GGYRCC 1 cut(s) 272
BanII GRGCYC 1 cut(s) 734
BauI CACGAG 1 cut(s) 627
BbsI GAAGAC 2 cut(s) 188, 527
BbvI GCAGC 5 cut(s) 327, 577, 944, 946, 949
BccI CCATC 1 cut(s) 46
BceAI ACGGC 2 cut(s) 31, 259
BciT130I CCWGG 1 cut(s) 828
BcnI CCSGG 1 cut(s) 903
BcoDI GTCTC 1 cut(s) 574
BfaI CTAG 4 cut(s) 566, 594, 615, 929
BfoI RGCGCY 2 cut(s) 276, 317
Bme1390I CCNGG 2 cut(s) 828, 903
Bme18I GGWCC 1 cut(s) 446
BmeRI GACNNNNNGTC 1 cut(s) 944
BmgT120I GGNCC 1 cut(s) 446
BmiI GGNNCC 1 cut(s) 274
BmrFI CCNGG 2 cut(s) 828, 903
BmsI GCATC 2 cut(s) 810, 979
BmtI GCTAGC 1 cut(s) 597
BpiI GAAGAC 2 cut(s) 188, 527
BpmI CTGGAG 1 cut(s) 161
BpuEI CTTGAG 2 cut(s) 131, 194
BpuMI CCSGG 1 cut(s) 903
Bsa29I ATCGAT 1 cut(s) 1005
BsaBI GATNNNNATC 1 cut(s) 775
BsaHI GRCGYC 1 cut(s) 273
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 492, 559
Bse8I GATNNNNATC 1 cut(s) 775
BseBI CCWGG 1 cut(s) 828
BseCI ATCGAT 1 cut(s) 1005
BseGI GGATG 2 cut(s) 825, 994
BseJI GATNNNNATC 1 cut(s) 775
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 2 cut(s) 492, 559
BseRI GAGGAG 7 cut(s) 55, 58, 61, 64, 67, 130, 710
BseXI GCAGC 5 cut(s) 327, 577, 944, 946, 949
BseYI CCCAGC 1 cut(s) 377
BshFI GGCC 1 cut(s) 28
BshNI GGYRCC 1 cut(s) 272
BshVI ATCGAT 1 cut(s) 1005
BsiSI CCGG 2 cut(s) 276, 903
BslFI GGGAC 1 cut(s) 776
BsmAI GTCTC 1 cut(s) 574
BsmFI GGGAC 1 cut(s) 776
BsnI GGCC 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 734
Bsp1407I TGTACA 1 cut(s) 387
Bsp143I GATC 2 cut(s) 394, 557
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 1 cut(s) 15
BspDI ATCGAT 1 cut(s) 1005
BspLI GGNNCC 1 cut(s) 274
BspOI GCTAGC 1 cut(s) 597
BspPI GGATC 2 cut(s) 402, 565
BspQI GCTCTTC 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 272
BsrFI RCCGGY 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 2 cut(s) 492, 559
BssAI RCCGGY 1 cut(s) 275
BssMI GATC 2 cut(s) 394, 557
BssNI GRCGYC 1 cut(s) 273
BssSI CACGAG 1 cut(s) 627
Bst2BI CACGAG 1 cut(s) 627
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 4 cut(s) 328, 550, 586, 942
Bst6I CTCTTC 3 cut(s) 17, 101, 224
BstACI GRCGYC 1 cut(s) 273
BstAPI GCANNNNNTGC 3 cut(s) 479, 959, 989
BstAUI TGTACA 1 cut(s) 387
BstC8I GCNNGC 3 cut(s) 277, 595, 867
BstDEI CTNAG 2 cut(s) 23, 575
BstF5I GGATG 2 cut(s) 825, 994
BstH2I RGCGCY 2 cut(s) 276, 317
BstHHI GCGC 3 cut(s) 6, 275, 316
BstKTI GATC 2 cut(s) 397, 560
BstMAI GTCTC 1 cut(s) 574
BstMBI GATC 2 cut(s) 394, 557
BstMWI GCNNNNNNNGC 8 cut(s) 276, 279, 458, 479, 506, 599, 959, 989
BstNI CCWGG 1 cut(s) 828
BstSCI CCNGG 2 cut(s) 826, 901
BstV1I GCAGC 5 cut(s) 327, 577, 944, 946, 949
BstV2I GAAGAC 2 cut(s) 188, 527
BstXI CCANNNNNNTGG 1 cut(s) 834
Bsu15I ATCGAT 1 cut(s) 1005
BsuRI GGCC 1 cut(s) 28
BsuTUI ATCGAT 1 cut(s) 1005
BtsCI GGATG 2 cut(s) 825, 994
BtsI GCAGTG 2 cut(s) 189, 480
BtsIMutI CAGTG 2 cut(s) 189, 480
Cac8I GCNNGC 3 cut(s) 277, 595, 867
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 3 cut(s) 6, 275, 316
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 446
ClaI ATCGAT 1 cut(s) 1005
Csp6I GTAC 2 cut(s) 324, 388
CviAII CATG 3 cut(s) 135, 523, 834
CviQI GTAC 2 cut(s) 324, 388
DdeI CTNAG 2 cut(s) 23, 575
DinI GGCGCC 1 cut(s) 274
DpnI GATC 2 cut(s) 396, 559
DpnII GATC 2 cut(s) 394, 557
DriI GACNNNNNGTC 1 cut(s) 944
Eam1104I CTCTTC 3 cut(s) 17, 101, 224
Eam1105I GACNNNNNGTC 1 cut(s) 944
EarI CTCTTC 3 cut(s) 17, 101, 224
EciI GGCGGA 2 cut(s) 33, 186
Eco24I GRGCYC 1 cut(s) 734
Eco32I GATATC 1 cut(s) 1003
Eco47I GGWCC 1 cut(s) 446
Eco57I CTGAAG 1 cut(s) 688
EcoRII CCWGG 1 cut(s) 826
EcoRV GATATC 1 cut(s) 1003
EcoT38I GRGCYC 1 cut(s) 734
EgeI GGCGCC 1 cut(s) 274
EheI GGCGCC 1 cut(s) 274
FaeI CATG 3 cut(s) 138, 526, 837
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 1 cut(s) 776
FatI CATG 3 cut(s) 134, 522, 833
FauI CCCGC 2 cut(s) 328, 445
FauNDI CATATG 1 cut(s) 796
FokI GGATG 2 cut(s) 832, 1001
FriOI GRGCYC 1 cut(s) 734
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 4 cut(s) 566, 594, 615, 929
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 274, 315
GsaI CCCAGC 1 cut(s) 381
GsuI CTGGAG 1 cut(s) 161
HaeII RGCGCY 2 cut(s) 276, 317
HaeIII GGCC 1 cut(s) 28
HapII CCGG 2 cut(s) 276, 903
HhaI GCGC 3 cut(s) 6, 275, 316
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 3 cut(s) 138, 526, 837
Hin6I GCGC 3 cut(s) 4, 273, 314
HinP1I GCGC 3 cut(s) 4, 273, 314
HincII GTYRAC 1 cut(s) 924
HindII GTYRAC 1 cut(s) 924
HinfI GANTC 4 cut(s) 415, 427, 502, 884
HpaII CCGG 2 cut(s) 276, 903
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 3 cut(s) 388, 423, 924
Hpy188I TCNGA 4 cut(s) 24, 609, 707, 889
Hpy188III TCNNGA 5 cut(s) 178, 412, 424, 629, 878
Hpy8I GTNNAC 3 cut(s) 388, 423, 924
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 4 cut(s) 70, 96, 418, 431
HpyCH4III ACNGT 4 cut(s) 328, 550, 586, 942
HpyCH4V TGCA 3 cut(s) 482, 674, 857
HpyF10VI GCNNNNNNNGC 8 cut(s) 276, 279, 458, 479, 506, 599, 959, 989
HpyF3I CTNAG 2 cut(s) 23, 575
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 3 cut(s) 138, 526, 837
HspAI GCGC 3 cut(s) 4, 273, 314
KasI GGCGCC 1 cut(s) 272
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
Kzo9I GATC 2 cut(s) 394, 557
LguI GCTCTTC 1 cut(s) 224
LmnI GCTCC 2 cut(s) 143, 199
Lsp1109I GCAGC 5 cut(s) 327, 577, 944, 946, 949
LweI GCATC 2 cut(s) 810, 979
MaeI CTAG 4 cut(s) 566, 594, 615, 929
MaeIII GTNAC 5 cut(s) 236, 550, 712, 946, 970
MalI GATC 2 cut(s) 396, 559
MboI GATC 2 cut(s) 394, 557
MboII GAAGA 5 cut(s) 4, 88, 193, 211, 527
MfeI CAATTG 1 cut(s) 675
MhlI GDGCHC 1 cut(s) 734
MluCI AATT 4 cut(s) 492, 561, 675, 873
Mly113I GGCGCC 1 cut(s) 273
MlyI GAGTC 1 cut(s) 421
MreI CGCCGGCG 1 cut(s) 275
MroNI GCCGGC 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 419
MseI TTAA 3 cut(s) 318, 402, 642
MspA1I CMGCKG 5 cut(s) 452, 509, 530, 919, 935
MspI CCGG 2 cut(s) 276, 903
MspR9I CCNGG 2 cut(s) 828, 903
MunI CAATTG 1 cut(s) 675
MvaI CCWGG 1 cut(s) 828
MwoI GCNNNNNNNGC 8 cut(s) 276, 279, 458, 479, 506, 599, 959, 989
NaeI GCCGGC 1 cut(s) 277
NarI GGCGCC 1 cut(s) 273
NciI CCSGG 1 cut(s) 903
NdeI CATATG 1 cut(s) 796
NdeII GATC 2 cut(s) 394, 557
NgoMIV GCCGGC 1 cut(s) 275
NheI GCTAGC 1 cut(s) 593
NlaIII CATG 3 cut(s) 138, 526, 837
NlaIV GGNNCC 1 cut(s) 274
NmuCI GTSAC 2 cut(s) 236, 946
NsbI TGCGCA 1 cut(s) 5
PciSI GCTCTTC 1 cut(s) 224
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PdiI GCCGGC 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 3 cut(s) 415, 502, 884
PflFI GACNNNGTC 1 cut(s) 523
PfoI TCCNGGA 1 cut(s) 826
PleI GAGTC 1 cut(s) 421
PluTI GGCGCC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 826
PspFI CCCAGC 1 cut(s) 377
PspGI CCWGG 1 cut(s) 826
PspN4I GGNNCC 1 cut(s) 274
PspPI GGNCC 1 cut(s) 446
PstNI CAGNNNCTG 1 cut(s) 554
PsyI GACNNNGTC 1 cut(s) 523
PvuII CAGCTG 4 cut(s) 509, 530, 919, 935
RsaI GTAC 2 cut(s) 325, 389
RsaNI GTAC 2 cut(s) 324, 388
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 3 cut(s) 318, 402, 642
Sau3AI GATC 2 cut(s) 394, 557
Sau96I GGNCC 1 cut(s) 446
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 2 cut(s) 828, 903
SduI GDGCHC 1 cut(s) 734
SetI ASST 7 cut(s) 325, 402, 511, 532, 921, 937, 961
SfaNI GCATC 2 cut(s) 810, 979
SfoI GGCGCC 1 cut(s) 274
SgrAI CRCCGGYG 1 cut(s) 275
SinI GGWCC 1 cut(s) 446
SmlI CTYRAG 2 cut(s) 146, 209
SmoI CTYRAG 2 cut(s) 146, 209
Sse9I AATT 4 cut(s) 492, 561, 675, 873
SspDI GGCGCC 1 cut(s) 272
SspMI CTAG 4 cut(s) 566, 594, 615, 929
StyD4I CCNGG 2 cut(s) 826, 901
TaaI ACNGT 4 cut(s) 328, 550, 586, 942
TaqI TCGA 1 cut(s) 1005
TasI AATT 4 cut(s) 492, 561, 675, 873
TatI WGTACW 1 cut(s) 387
TauI GCSGC 7 cut(s) 174, 202, 249, 270, 273, 282, 377
TfiI GAWTC 3 cut(s) 415, 502, 884
Tru1I TTAA 3 cut(s) 318, 402, 642
Tru9I TTAA 3 cut(s) 318, 402, 642
TscAI CASTG 2 cut(s) 196, 480
TseFI GTSAC 2 cut(s) 236, 946
TseI GCWGC 5 cut(s) 340, 590, 932, 959, 962
Tsp45I GTSAC 2 cut(s) 236, 946
TspGWI ACGGA 1 cut(s) 713
TspRI CASTG 2 cut(s) 196, 480
Tth111I GACNNNGTC 1 cut(s) 523
VpaK11BI GGWCC 1 cut(s) 446
XapI RAATTY 1 cut(s) 492
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 4 cut(s) 566, 594, 615, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.