Rw6G001210

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008617
Physical Location & Seq
Forward (+)
1729655 .. 1734670
5016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G001210.1

Sequence Viewer

Length: 945 bp
ATGCGCACCCTCTTCCTCACTCTGAGGCCACCACCACCATCTCCGCCGTGCCTCTCTCCCTTCTCCTCCTCCTCCTCCCACCCTTCTTTCCTCTTCAAACCCAACCTCCACCGCTTCCACTTCCTCTCACCATGTTCCCTCAAGTGGCTCTTCAATAATCCCAAGAGTGACGGCGGCGGCGAGATTGAAAAAATCGGCGGCGGCGGCGGCGGCGGTGGTGGTGATGATGAGGGCGAGGGTGGGTTGGCGCTTAAAGGTACTGTCTTGGCGGGGCTGTTGTTGGTTGGGCTTGTTGGTGGGTTTGGGGCGGCTGGGTATGTGTACAAGGATCAGGTTAATGCCTTCTTGAATCAGTTTACGACTCTCATTGAAGGGTATGGTCCAGCGGGATATGCTTTGTTTGTTGCAGTTTATGCAGGACTGGAAATTCTTGCGATTCCAGCTGTTCCATTGACCATGTCAGCTGGTCTTCTTTTTGGCACAGTTACTGGATCAATTCTAGTCTCTCTAAGCGGAACGGTGGCTGCTAGCGTTGCTTTTCTGATTGCTAGATATTTTGCTCGTGAACGTATTCTTAAAATGGTTGAGGGAAACAAAAAGTTTCTTGCAATTGATAAGGCTATTGGAGAAAATGGCTTCAGAGTTGTTACTCTCCTCCGTTTGAGCCCTTTGCTTCCTTTTTCTCTGGGGAACTATTTATATGGATTGACATCTGTAAAGTTTGTCCCATATGTGTTGGGAAGTTGGTTGGGGATGCTTCCAGGAACATGGGCTTATGTGAGTGCTGGTGCATTTGGGCGAGCCATAATTCAAGAGGAATCTGATGTTGGTTTGCCCGGAGGAAATGGTCAGCTGTTGACACTAGCAGCTGGACTGTTAGTCACAGCATTAGCTGCTGCTTATGTAACACGATTAGCAAAGGATGCTGTAAAAGATATCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

314

Amino Acids

32.76

Weight (kDa)

8.76

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 146 - 264 1.6e-23 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AclWI GGATC 2 cut(s) 336, 499
AcsI RAATTY 1 cut(s) 426
AcuI CTGAAG 1 cut(s) 622
AfaI GTAC 2 cut(s) 259, 323
AfiI CCNNNNNNNGG 1 cut(s) 144
AgsI TTSAA 6 cut(s) 97, 154, 188, 349, 371, 812
AhdI GACNNNNNGTC 1 cut(s) 878
AjnI CCWGG 1 cut(s) 760
AjuI GAANNNNNNNTTGG 2 cut(s) 810, 842
AluBI AGCT 5 cut(s) 443, 464, 853, 869, 893
AluI AGCT 5 cut(s) 443, 464, 853, 869, 893
Alw26I GTCTC 1 cut(s) 508
AlwI GGATC 2 cut(s) 336, 499
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 4 cut(s) 524, 866, 893, 896
ApoI RAATTY 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 570
AspLEI GCGC 2 cut(s) 6, 250
AspS9I GGNCC 1 cut(s) 380
AsuC2I CCSGG 1 cut(s) 837
AsuHPI GGTGA 2 cut(s) 120, 233
AsuNHI GCTAGC 1 cut(s) 527
AvaII GGWCC 1 cut(s) 380
BanII GRGCYC 1 cut(s) 668
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 461
BbvI GCAGC 4 cut(s) 511, 878, 880, 883
BccI CCATC 1 cut(s) 46
BceAI ACGGC 2 cut(s) 31, 187
BciT130I CCWGG 1 cut(s) 762
BcnI CCSGG 1 cut(s) 837
BcoDI GTCTC 1 cut(s) 508
BfaI CTAG 4 cut(s) 500, 528, 549, 863
BfoI RGCGCY 1 cut(s) 251
Bme1390I CCNGG 2 cut(s) 762, 837
Bme18I GGWCC 1 cut(s) 380
BmeRI GACNNNNNGTC 1 cut(s) 878
BmgT120I GGNCC 1 cut(s) 380
BmrFI CCNGG 2 cut(s) 762, 837
BmsI GCATC 2 cut(s) 744, 913
BmtI GCTAGC 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 461
BpuEI CTTGAG 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 837
Bsa29I ATCGAT 1 cut(s) 939
BsaBI GATNNNNATC 1 cut(s) 709
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse1I ACTGG 2 cut(s) 426, 493
Bse8I GATNNNNATC 1 cut(s) 709
BseBI CCWGG 1 cut(s) 762
BseCI ATCGAT 1 cut(s) 939
BseGI GGATG 2 cut(s) 759, 928
BseJI GATNNNNATC 1 cut(s) 709
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 2 cut(s) 426, 493
BseRI GAGGAG 5 cut(s) 55, 58, 61, 64, 644
BseXI GCAGC 4 cut(s) 511, 878, 880, 883
BseYI CCCAGC 1 cut(s) 311
BshFI GGCC 1 cut(s) 28
BshVI ATCGAT 1 cut(s) 939
BsiSI CCGG 1 cut(s) 837
BslFI GGGAC 1 cut(s) 710
BslI CCNNNNNNNGG 1 cut(s) 144
BsmAI GTCTC 1 cut(s) 508
BsmFI GGGAC 1 cut(s) 710
BsnI GGCC 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 668
Bsp1407I TGTACA 1 cut(s) 321
Bsp143I GATC 2 cut(s) 328, 491
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 1 cut(s) 15
BspDI ATCGAT 1 cut(s) 939
BspOI GCTAGC 1 cut(s) 531
BspPI GGATC 2 cut(s) 336, 499
BspQI GCTCTTC 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 321
BsrI ACTGG 2 cut(s) 426, 493
BssMI GATC 2 cut(s) 328, 491
BssSI CACGAG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 1 cut(s) 762
Bst4CI ACNGT 4 cut(s) 262, 484, 520, 876
Bst6I CTCTTC 3 cut(s) 17, 98, 155
BstAPI GCANNNNNTGC 3 cut(s) 413, 893, 923
BstAUI TGTACA 1 cut(s) 321
BstC8I GCNNGC 2 cut(s) 529, 801
BstDEI CTNAG 2 cut(s) 23, 509
BstF5I GGATG 2 cut(s) 759, 928
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 2 cut(s) 6, 250
BstKTI GATC 2 cut(s) 331, 494
BstMAI GTCTC 1 cut(s) 508
BstMBI GATC 2 cut(s) 328, 491
BstMWI GCNNNNNNNGC 9 cut(s) 204, 207, 210, 392, 413, 440, 533, 893, 923
BstNI CCWGG 1 cut(s) 762
BstSCI CCNGG 2 cut(s) 760, 835
BstV1I GCAGC 4 cut(s) 511, 878, 880, 883
BstV2I GAAGAC 1 cut(s) 461
BstXI CCANNNNNNTGG 1 cut(s) 768
Bsu15I ATCGAT 1 cut(s) 939
BsuRI GGCC 1 cut(s) 28
BsuTUI ATCGAT 1 cut(s) 939
BtsCI GGATG 2 cut(s) 759, 928
Cac8I GCNNGC 2 cut(s) 529, 801
CaiI CAGNNNCTG 1 cut(s) 488
CfoI GCGC 2 cut(s) 6, 250
Cfr13I GGNCC 1 cut(s) 380
ClaI ATCGAT 1 cut(s) 939
Csp6I GTAC 2 cut(s) 258, 322
CviAII CATG 3 cut(s) 132, 457, 768
CviQI GTAC 2 cut(s) 258, 322
DdeI CTNAG 2 cut(s) 23, 509
DpnI GATC 2 cut(s) 330, 493
DpnII GATC 2 cut(s) 328, 491
DriI GACNNNNNGTC 1 cut(s) 878
Eam1104I CTCTTC 3 cut(s) 17, 98, 155
Eam1105I GACNNNNNGTC 1 cut(s) 878
EarI CTCTTC 3 cut(s) 17, 98, 155
EciI GGCGGA 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 668
Eco32I GATATC 1 cut(s) 937
Eco47I GGWCC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 760
EcoRV GATATC 1 cut(s) 937
EcoT38I GRGCYC 1 cut(s) 668
FaeI CATG 3 cut(s) 135, 460, 771
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 710
FatI CATG 3 cut(s) 131, 456, 767
FauI CCCGC 2 cut(s) 262, 379
FauNDI CATATG 1 cut(s) 730
FokI GGATG 2 cut(s) 766, 935
FriOI GRGCYC 1 cut(s) 668
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 4 cut(s) 500, 528, 549, 863
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 2 cut(s) 5, 249
GsaI CCCAGC 1 cut(s) 315
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 1 cut(s) 28
HapII CCGG 1 cut(s) 837
HhaI GCGC 2 cut(s) 6, 250
Hin1II CATG 3 cut(s) 135, 460, 771
Hin6I GCGC 2 cut(s) 4, 248
HinP1I GCGC 2 cut(s) 4, 248
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HinfI GANTC 4 cut(s) 349, 361, 436, 818
HpaII CCGG 1 cut(s) 837
HphI GGTGA 2 cut(s) 120, 233
Hpy166II GTNNAC 4 cut(s) 322, 357, 566, 858
Hpy188I TCNGA 4 cut(s) 24, 543, 641, 823
Hpy188III TCNNGA 3 cut(s) 346, 563, 812
Hpy8I GTNNAC 4 cut(s) 322, 357, 566, 858
HpyAV CCTTC 4 cut(s) 70, 93, 352, 365
HpyCH4III ACNGT 4 cut(s) 262, 484, 520, 876
HpyCH4IV ACGT 1 cut(s) 568
HpyCH4V TGCA 4 cut(s) 407, 416, 608, 791
HpyF10VI GCNNNNNNNGC 9 cut(s) 204, 207, 210, 392, 413, 440, 533, 893, 923
HpyF3I CTNAG 2 cut(s) 23, 509
HpySE526I ACGT 1 cut(s) 568
Hsp92II CATG 3 cut(s) 135, 460, 771
HspAI GCGC 2 cut(s) 4, 248
Kzo9I GATC 2 cut(s) 328, 491
LguI GCTCTTC 1 cut(s) 155
Lsp1109I GCAGC 4 cut(s) 511, 878, 880, 883
LweI GCATC 2 cut(s) 744, 913
MaeI CTAG 4 cut(s) 500, 528, 549, 863
MaeII ACGT 1 cut(s) 568
MaeIII GTNAC 5 cut(s) 167, 484, 646, 880, 904
MalI GATC 2 cut(s) 330, 493
MboI GATC 2 cut(s) 328, 491
MboII GAAGA 4 cut(s) 4, 85, 142, 461
MfeI CAATTG 1 cut(s) 609
MhlI GDGCHC 1 cut(s) 668
MluCI AATT 4 cut(s) 426, 495, 609, 807
MlyI GAGTC 1 cut(s) 355
MroXI GAANNNNTTC 1 cut(s) 570
MseI TTAA 3 cut(s) 252, 336, 576
MspA1I CMGCKG 5 cut(s) 386, 443, 464, 853, 869
MspI CCGG 1 cut(s) 837
MspR9I CCNGG 2 cut(s) 762, 837
MunI CAATTG 1 cut(s) 609
MvaI CCWGG 1 cut(s) 762
MwoI GCNNNNNNNGC 9 cut(s) 204, 207, 210, 392, 413, 440, 533, 893, 923
NciI CCSGG 1 cut(s) 837
NdeI CATATG 1 cut(s) 730
NdeII GATC 2 cut(s) 328, 491
NheI GCTAGC 1 cut(s) 527
NlaIII CATG 3 cut(s) 135, 460, 771
NmuCI GTSAC 2 cut(s) 167, 880
NsbI TGCGCA 1 cut(s) 5
PciSI GCTCTTC 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 3 cut(s) 349, 436, 818
PflFI GACNNNGTC 1 cut(s) 457
PfoI TCCNGGA 1 cut(s) 760
PleI GAGTC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 760
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 1 cut(s) 760
PspPI GGNCC 1 cut(s) 380
PstNI CAGNNNCTG 1 cut(s) 488
PsyI GACNNNGTC 1 cut(s) 457
PvuII CAGCTG 4 cut(s) 443, 464, 853, 869
RsaI GTAC 2 cut(s) 259, 323
RsaNI GTAC 2 cut(s) 258, 322
SapI GCTCTTC 1 cut(s) 155
SaqAI TTAA 3 cut(s) 252, 336, 576
Sau3AI GATC 2 cut(s) 328, 491
Sau96I GGNCC 1 cut(s) 380
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 2 cut(s) 762, 837
SduI GDGCHC 1 cut(s) 668
SetI ASST 9 cut(s) 108, 259, 336, 445, 466, 571, 855, 871, 895
SfaNI GCATC 2 cut(s) 744, 913
SinI GGWCC 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 4 cut(s) 426, 495, 609, 807
SspMI CTAG 4 cut(s) 500, 528, 549, 863
StyD4I CCNGG 2 cut(s) 760, 835
TaaI ACNGT 4 cut(s) 262, 484, 520, 876
TaiI ACGT 1 cut(s) 571
TaqI TCGA 1 cut(s) 939
TasI AATT 4 cut(s) 426, 495, 609, 807
TatI WGTACW 1 cut(s) 321
TauI GCSGC 8 cut(s) 177, 180, 201, 204, 207, 210, 213, 311
TfiI GAWTC 3 cut(s) 349, 436, 818
Tru1I TTAA 3 cut(s) 252, 336, 576
Tru9I TTAA 3 cut(s) 252, 336, 576
TseFI GTSAC 2 cut(s) 167, 880
TseI GCWGC 4 cut(s) 524, 866, 893, 896
Tsp45I GTSAC 2 cut(s) 167, 880
TspGWI ACGGA 1 cut(s) 647
Tth111I GACNNNGTC 1 cut(s) 457
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 4 cut(s) 500, 528, 549, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.