FvH4_2g00910

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008617
Physical Location & Seq
Forward (+)
967533 .. 973182
5650 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00910.t1

Sequence Viewer

Length: 984 bp
ATGCGCACCCTCTTCCTCACTCTGAGGCCACCTCCATCTCCGCCGTCCCTCTCTCCCTTCTCCTCCCCCAAACCCAACCACCACCGCCTCCACTTCCTCTCCCCATGCTCCTCTCTCAAGCAGACCAACACCAAGACCACCCGCCTCCAGAAGACCACCGCCGCTCCGCCGCAGAGCCTCAAGTGGCTCTTCAGCAACCCCAAGAGCGACGACGGCGCCGAGATTGAGAAAAGCGGCCGAGGCGGAGTTGGCGGCGGCGGTGATGATGAGGGCGAGGGTGAGTTCGCGCTTAAAGGTACAATCTTGGCGGGGCTGTTGTTGGTTGGGTTGGTTGGGGGGTTTGGTGCGGCTGGGTATGTGTATAAGGATCAGGTTAATGCCTTCTTGAATCAGTTTACGACTCTCATTGAAGGTTATGGTCCAGCAGGATATGCATTGTTTATAGCAGTTTATGCAGGACTGGAAATCCTAGCGATTCCAGCCGTTCCATTGACCATGTCAGCTGGTCTTCTTTTTGGCACAGTTGTTGGCTCAATTTTAGTCTCTATAAGCGGAACGGTGGCCGCTAGCGTTGCTTTTCTGATAGCTAGATATTTTGCTCGTGAACGTATTCTTAAAATGGTTGAGGGAAACAAGAAGTTTCTTGCAATCGACAAGGCTATCGGGGAAAATGGCTTCAGAGTTGTCACTCTCCTCCGTTTGAGCCCTTTGCTTCCTTTTTCTCTGGGGAACTATTTATATGGACTGACATCTGTAAAGTTTGTCCCTTATGTCTTGGGAAGTTGGTTGGGGATGCTTCCAGGAACATGGGCTTATGTGAGTGCAGGTGCATTTGGGCGAGCCATAATTCAAGAAGAATCTGATGTTGGATTGCCAGGAGGAAATGGTCAGCTGTTGACGCTAGCCGCTGGACTATTAGTCACAGCATTAGCTGCTGCTTATGTAACACGATTAGCAAAGGATGCTGTAAAAGATATTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

328

Amino Acids

34.42

Weight (kDa)

9.28

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 159 - 277 1.9e-23 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 815
Acc16I TGCGCA 1 cut(s) 5
Acc36I ACCTGC 1 cut(s) 815
AccB1I GGYRCC 1 cut(s) 215
AccBSI CCGCTC 1 cut(s) 164
AccII CGCG 1 cut(s) 287
AclWI GGATC 1 cut(s) 375
AcoI YGGCCR 2 cut(s) 235, 561
AcuI CTGAAG 2 cut(s) 175, 661
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 1 cut(s) 298
AgsI TTSAA 3 cut(s) 388, 410, 851
AhdI GACNNNNNGTC 1 cut(s) 917
AjnI CCWGG 2 cut(s) 799, 874
AjuI GAANNNNNNNTTGG 2 cut(s) 849, 881
AluBI AGCT 4 cut(s) 503, 587, 892, 932
AluI AGCT 4 cut(s) 503, 587, 892, 932
Alw26I GTCTC 1 cut(s) 547
AlwI GGATC 1 cut(s) 375
AoxI GGCC 3 cut(s) 26, 235, 561
ApeKI GCWGC 2 cut(s) 932, 935
Asp700I GAANNNNTTC 1 cut(s) 609
AspLEI GCGC 3 cut(s) 6, 218, 289
AspS9I GGNCC 1 cut(s) 419
AsuHPI GGTGA 2 cut(s) 272, 290
AsuNHI GCTAGC 2 cut(s) 566, 901
AvaII GGWCC 1 cut(s) 419
BanI GGYRCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 707
BauI CACGAG 1 cut(s) 600
BbsI GAAGAC 2 cut(s) 158, 500
BbvI GCAGC 2 cut(s) 919, 922
BccI CCATC 1 cut(s) 43
BceAI ACGGC 3 cut(s) 28, 229, 467
BciT130I CCWGG 2 cut(s) 801, 876
BcoDI GTCTC 1 cut(s) 547
BfaI CTAG 4 cut(s) 470, 567, 588, 902
BfoI RGCGCY 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 815
Bme1390I CCNGG 2 cut(s) 801, 876
Bme18I GGWCC 1 cut(s) 419
BmeRI GACNNNNNGTC 1 cut(s) 917
BmgT120I GGNCC 1 cut(s) 419
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 2 cut(s) 801, 876
BmsI GCATC 2 cut(s) 783, 952
BmtI GCTAGC 2 cut(s) 570, 905
BpiI GAAGAC 2 cut(s) 158, 500
BplI GAGNNNNNCTC 2 cut(s) 16, 48
BpmI CTGGAG 1 cut(s) 131
BpuEI CTTGAG 2 cut(s) 101, 164
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 1 cut(s) 238
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 148, 178
Bse1I ACTGG 1 cut(s) 465
BseBI CCWGG 2 cut(s) 801, 876
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 2 cut(s) 798, 967
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 465
BseRI GAGGAG 3 cut(s) 52, 100, 683
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 2 cut(s) 919, 922
BseYI CCCAGC 1 cut(s) 350
BsgI GTGCAG 1 cut(s) 843
Bsh1236I CGCG 1 cut(s) 287
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 3 cut(s) 28, 237, 563
BshNI GGYRCC 1 cut(s) 215
BsiEI CGRYCG 1 cut(s) 238
BslFI GGGAC 2 cut(s) 31, 749
BsmAI GTCTC 1 cut(s) 547
BsmFI GGGAC 2 cut(s) 31, 749
BsnI GGCC 3 cut(s) 28, 237, 563
Bsp1286I GDGCHC 1 cut(s) 707
Bsp143I GATC 1 cut(s) 367
BspANI GGCC 3 cut(s) 28, 237, 563
BspCNI CTCAG 1 cut(s) 15
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 1 cut(s) 217
BspMI ACCTGC 1 cut(s) 815
BspOI GCTAGC 2 cut(s) 570, 905
BspPI GGATC 1 cut(s) 375
BspQI GCTCTTC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 164
BsrI ACTGG 1 cut(s) 465
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 1 cut(s) 367
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 600
Bst2BI CACGAG 1 cut(s) 600
Bst2UI CCWGG 2 cut(s) 801, 876
Bst4CI ACNGT 2 cut(s) 523, 559
Bst6I CTCTTC 2 cut(s) 17, 194
BstACI GRCGYC 1 cut(s) 216
BstAPI GCANNNNNTGC 4 cut(s) 431, 452, 932, 962
BstC8I GCNNGC 3 cut(s) 568, 840, 903
BstDEI CTNAG 1 cut(s) 23
BstF5I GGATG 2 cut(s) 798, 967
BstFNI CGCG 1 cut(s) 287
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 3 cut(s) 6, 218, 289
BstKTI GATC 1 cut(s) 370
BstMAI GTCTC 1 cut(s) 547
BstMBI GATC 1 cut(s) 367
BstMCI CGRYCG 1 cut(s) 238
BstNI CCWGG 2 cut(s) 801, 876
BstSCI CCNGG 2 cut(s) 799, 874
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 2 cut(s) 919, 922
BstV2I GAAGAC 2 cut(s) 158, 500
BstXI CCANNNNNNTGG 1 cut(s) 807
BstZI CGGCCG 1 cut(s) 235
BsuRI GGCC 3 cut(s) 28, 237, 563
BtsCI GGATG 2 cut(s) 798, 967
BveI ACCTGC 1 cut(s) 815
Cac8I GCNNGC 3 cut(s) 568, 840, 903
CfoI GCGC 3 cut(s) 6, 218, 289
Cfr13I GGNCC 1 cut(s) 419
CseI GACGC 1 cut(s) 907
Csp6I GTAC 1 cut(s) 297
CviAII CATG 3 cut(s) 105, 496, 807
CviQI GTAC 1 cut(s) 297
DdeI CTNAG 1 cut(s) 23
DinI GGCGCC 1 cut(s) 217
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
DriI GACNNNNNGTC 1 cut(s) 917
EaeI YGGCCR 2 cut(s) 235, 561
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 2 cut(s) 17, 194
Eam1105I GACNNNNNGTC 1 cut(s) 917
EarI CTCTTC 2 cut(s) 17, 194
EciI GGCGGA 3 cut(s) 30, 156, 258
EclXI CGGCCG 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 707
Eco47I GGWCC 1 cut(s) 419
Eco52I CGGCCG 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 175, 661
EcoRII CCWGG 2 cut(s) 799, 874
EcoT22I ATGCAT 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 707
EgeI GGCGCC 1 cut(s) 217
EheI GGCGCC 1 cut(s) 217
FaeI CATG 3 cut(s) 108, 499, 810
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 2 cut(s) 31, 749
FatI CATG 3 cut(s) 104, 495, 806
FauI CCCGC 2 cut(s) 149, 301
FokI GGATG 2 cut(s) 805, 974
FriOI GRGCYC 1 cut(s) 707
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 4 cut(s) 470, 567, 588, 902
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 217, 288
GsaI CCCAGC 1 cut(s) 354
GsuI CTGGAG 1 cut(s) 131
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 3 cut(s) 28, 237, 563
HgaI GACGC 1 cut(s) 907
HhaI GCGC 3 cut(s) 6, 218, 289
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 3 cut(s) 108, 499, 810
Hin6I GCGC 3 cut(s) 4, 216, 287
HinP1I GCGC 3 cut(s) 4, 216, 287
HincII GTYRAC 1 cut(s) 897
HindII GTYRAC 1 cut(s) 897
HinfI GANTC 4 cut(s) 388, 400, 475, 857
HphI GGTGA 2 cut(s) 272, 290
Hpy166II GTNNAC 3 cut(s) 396, 605, 897
Hpy188I TCNGA 4 cut(s) 24, 582, 680, 862
Hpy188III TCNNGA 4 cut(s) 148, 385, 602, 851
Hpy8I GTNNAC 3 cut(s) 396, 605, 897
Hpy99I CGWCG 2 cut(s) 212, 215
HpyAV CCTTC 3 cut(s) 67, 391, 404
HpyCH4III ACNGT 2 cut(s) 523, 559
HpyCH4IV ACGT 1 cut(s) 607
HpyCH4V TGCA 5 cut(s) 434, 455, 647, 824, 830
HpyF3I CTNAG 1 cut(s) 23
HpySE526I ACGT 1 cut(s) 607
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 3 cut(s) 108, 499, 810
HspAI GCGC 3 cut(s) 4, 216, 287
KasI GGCGCC 1 cut(s) 215
Kzo9I GATC 1 cut(s) 367
LguI GCTCTTC 1 cut(s) 194
LmnI GCTCC 2 cut(s) 113, 169
Lsp1109I GCAGC 2 cut(s) 919, 922
LweI GCATC 2 cut(s) 783, 952
MaeI CTAG 4 cut(s) 470, 567, 588, 902
MaeII ACGT 1 cut(s) 607
MaeIII GTNAC 3 cut(s) 685, 919, 943
MalI GATC 1 cut(s) 369
MbiI CCGCTC 1 cut(s) 164
MboI GATC 1 cut(s) 367
MboII GAAGA 5 cut(s) 4, 163, 181, 500, 866
MhlI GDGCHC 1 cut(s) 707
MluCI AATT 2 cut(s) 534, 846
Mly113I GGCGCC 1 cut(s) 216
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 847
Mph1103I ATGCAT 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 3 cut(s) 291, 375, 615
MspA1I CMGCKG 3 cut(s) 503, 892, 908
MspR9I CCNGG 2 cut(s) 801, 876
MvaI CCWGG 2 cut(s) 801, 876
MvnI CGCG 1 cut(s) 287
NarI GGCGCC 1 cut(s) 216
NdeII GATC 1 cut(s) 367
NheI GCTAGC 2 cut(s) 566, 901
NlaIII CATG 3 cut(s) 108, 499, 810
NlaIV GGNNCC 1 cut(s) 217
NmeAIII GCCGAG 2 cut(s) 244, 263
NmuCI GTSAC 2 cut(s) 685, 919
NsbI TGCGCA 1 cut(s) 5
NsiI ATGCAT 1 cut(s) 436
PaqCI CACCTGC 1 cut(s) 815
PciSI GCTCTTC 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 3 cut(s) 388, 475, 857
PflFI GACNNNGTC 1 cut(s) 496
PfoI TCCNGGA 1 cut(s) 799
PleI GAGTC 1 cut(s) 394
PluTI GGCGCC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 394
Psp6I CCWGG 2 cut(s) 799, 874
PspFI CCCAGC 1 cut(s) 350
PspGI CCWGG 2 cut(s) 799, 874
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 1 cut(s) 419
PsyI GACNNNGTC 1 cut(s) 496
PvuII CAGCTG 2 cut(s) 503, 892
RsaI GTAC 1 cut(s) 298
RsaNI GTAC 1 cut(s) 297
SapI GCTCTTC 1 cut(s) 194
SaqAI TTAA 3 cut(s) 291, 375, 615
Sau3AI GATC 1 cut(s) 367
Sau96I GGNCC 1 cut(s) 419
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 2 cut(s) 801, 876
SduI GDGCHC 1 cut(s) 707
SfaNI GCATC 2 cut(s) 783, 952
SfoI GGCGCC 1 cut(s) 217
SinI GGWCC 1 cut(s) 419
SmlI CTYRAG 2 cut(s) 116, 179
SmoI CTYRAG 2 cut(s) 116, 179
Sse9I AATT 2 cut(s) 534, 846
SspDI GGCGCC 1 cut(s) 215
SspMI CTAG 4 cut(s) 470, 567, 588, 902
StyD4I CCNGG 2 cut(s) 799, 874
TaaI ACNGT 2 cut(s) 523, 559
TaiI ACGT 1 cut(s) 610
TaqI TCGA 1 cut(s) 651
TasI AATT 2 cut(s) 534, 846
TauI GCSGC 8 cut(s) 164, 172, 237, 255, 258, 350, 566, 908
TfiI GAWTC 3 cut(s) 388, 475, 857
Tru1I TTAA 3 cut(s) 291, 375, 615
Tru9I TTAA 3 cut(s) 291, 375, 615
TseFI GTSAC 2 cut(s) 685, 919
TseI GCWGC 2 cut(s) 932, 935
Tsp45I GTSAC 2 cut(s) 685, 919
TspGWI ACGGA 1 cut(s) 686
Tth111I GACNNNGTC 1 cut(s) 496
VpaK11BI GGWCC 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 609
XspI CTAG 4 cut(s) 470, 567, 588, 902
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.