AT1G23550

Encodes a protein with similarity to RCD1 but without the WWE domain. The protein does have a PARP signature upstream of the C-terminal protein interaction domain. The PARP signature may bind NAD and attach the ADP-ribose-moiety from NAD to the target molecule. Its presence suggests a role for the protein in ADP ribosylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010041
Physical Location & Seq
Forward (+)
8350728 .. 8352791
2064 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23550.1

Sequence Viewer

Length: 972 bp
ATGGCGGCGCAGGTCGAAATTGAGGACCAAACGTCCGTCACCAACCTTGATAATGGCGAAATATTCGATTCAATCTCCGACGATGCTGATTCTTCTGTTTCTCACGCCGGATCTTCTTTCTCTTCCTCTTCACTGATCCTTCTCGGCGAAGGAAACCCAGAGCACGACGTTATCAAGACGTGTTTACTCTCGGGAATGGGTGTTGTTTCTAGCGACACGACGATCGTTACGATTAGTAAAAACTCTTCGGAACGAGGAATCACGACGAGAGCCAAGTTTTTGGCGTTTAGGATTTTCACTGACGCTGTTGCGAGGAAACACGGCGGAGATGCTAATGTGAAATATGGTTGGTACGCTGGTTCTAGAGATGAGATTCAAAGAATCATCTCTTACGGATTTAGCAATCGTGATGTTGGAAAATTTGAAAATGATGGTGGTTCTCATGGAATTGGAATCCATTTGGTTCCTTCCAAGTGTTCTCTACTTGCGGCTTCAGCTACAGAGCAAGATGAAGAAGGTTTACGATATCTTCTGTTATGCCGTGTAATCTTAGGGAAACCTGAGATAATCATCTCCGGCTCTAAACAATCGTATCCGAGTTCTGCTGAGTTTGATTCTGGTGTGGATGATTTACACAATCCTAGAAACTATGTGATTTGGAGTTGTAACATGAATTCGTGTATTCTTCCGAGTTATATCGTCAGTTTTAGGTCTCCTAGACTGAGAGTGAGTAGAGGTGGTTTTGCTTCGAGACCAAGCTCGCCGTGGGTTAGTTTTGCTTCCTTGATGTCAATGTTGTCTACATCAATGGATCCTTCAAGAATGAACTTGATCATCCGAACTTACGATGATTTTCGGAAACGGAAGATTAGGAGAGACCAATTGGTAAGGAAGATGAGAGAAGTGGCTGGAGATAATCTTTTGGCTGAGATAATCAAGAATCACAAAAACAGAAACAAGGTCACGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

323

Amino Acids

35.6

Weight (kDa)

8.22

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 251 - 316 2e-26 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23550 AT1G70440 AT1G70440
fragaria_vesca FvH4_4g34350
malus_domestica MD13G1092800.v1.1 MD16G1093700.v1.1
prunus_persica Prupe.1G252800_v2.0.a1
pyrus_communis pycom13g08070 pycom13g08080 pycom16g07970
rosa_chinensis RchiOBHm_Chr4g0443761
rosa_laevigata RLG00000005892
rosa_multiflora Rmu_co8242545.1_g000001 Rmu_sc0042927.1_g000001
rosa_roxburghii Rroxscaffold_5G00384330
rosa_rugosa Rorug04G0347100
rosa_samantha Rh4AG407300 Rh4BG418200 Rh4CG432500 Rh4DG413500
rosa_wichuraiana Rw4G034980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 800
AciI CCGC 3 cut(s) 5, 324, 488
AclWI GGATC 4 cut(s) 118, 130, 806, 819
AcsI RAATTY 2 cut(s) 419, 673
AcuI CTGAAG 1 cut(s) 477
AfaI GTAC 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 4 cut(s) 72, 377, 425, 819
AhdI GACNNNNNGTC 1 cut(s) 31
AjiI CACGTC 1 cut(s) 180
AluBI AGCT 2 cut(s) 497, 759
AluI AGCT 2 cut(s) 497, 759
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 3 cut(s) 717, 745, 870
AlwI GGATC 4 cut(s) 118, 130, 806, 819
Ama87I CYCGRG 1 cut(s) 190
ApoI RAATTY 2 cut(s) 419, 673
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 1 cut(s) 31
AvaI CYCGRG 1 cut(s) 190
AvaII GGWCC 1 cut(s) 25
BaeI ACNNNNGTAYC 4 cut(s) 343, 376, 575, 608
BamHI GGATCC 1 cut(s) 811
BarI GAAGNNNNNNTAC 4 cut(s) 229, 261, 504, 536
Bbv12I GWGCWC 1 cut(s) 165
BccI CCATC 1 cut(s) 425
BceAI ACGGC 3 cut(s) 337, 525, 748
BciVI GTATCC 1 cut(s) 603
BclI TGATCA 1 cut(s) 831
BcoDI GTCTC 3 cut(s) 717, 745, 870
BfaI CTAG 4 cut(s) 210, 363, 642, 717
BfmI CTRYAG 1 cut(s) 498
BfuI GTATCC 1 cut(s) 603
BisI GCNGC 2 cut(s) 6, 489
BlsI GCNGC 2 cut(s) 7, 490
Bme18I GGWCC 1 cut(s) 25
BmeRI GACNNNNNGTC 1 cut(s) 31
BmeT110I CYCGRG 1 cut(s) 190
BmgBI CACGTC 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 2 cut(s) 465, 813
BmsI GCATC 2 cut(s) 73, 319
BpmI CTGGAG 1 cut(s) 930
BsaBI GATNNNNATC 1 cut(s) 569
BsaI GGTCTC 3 cut(s) 717, 745, 870
BsaJI CCNNGG 1 cut(s) 764
Bse8I GATNNNNATC 1 cut(s) 569
BseDI CCNNGG 1 cut(s) 764
BseGI GGATG 2 cut(s) 631, 834
BseJI GATNNNNATC 1 cut(s) 569
BseMII CTCAG 4 cut(s) 552, 597, 713, 918
Bsh1285I CGRYCG 1 cut(s) 225
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 190
BsiSI CCGG 2 cut(s) 108, 576
BsmAI GTCTC 3 cut(s) 717, 745, 870
Bso31I GGTCTC 3 cut(s) 717, 745, 870
BsoBI CYCGRG 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 5 cut(s) 110, 135, 222, 811, 831
BspACI CCGC 3 cut(s) 5, 324, 488
BspCNI CTCAG 4 cut(s) 553, 598, 714, 919
BspLI GGNNCC 2 cut(s) 465, 813
BspPI GGATC 4 cut(s) 118, 130, 806, 819
BspTNI GGTCTC 3 cut(s) 717, 745, 870
BssECI CCNNGG 1 cut(s) 764
BssMI GATC 5 cut(s) 110, 135, 222, 811, 831
Bst6I CTCTTC 3 cut(s) 127, 133, 250
BstC8I GCNNGC 1 cut(s) 761
BstDEI CTNAG 5 cut(s) 550, 561, 606, 722, 927
BstDSI CCRYGG 1 cut(s) 764
BstF5I GGATG 2 cut(s) 631, 834
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 5 cut(s) 113, 138, 225, 814, 834
BstMAI GTCTC 3 cut(s) 717, 745, 870
BstMBI GATC 5 cut(s) 110, 135, 222, 811, 831
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 1 cut(s) 494
BstSFI CTRYAG 1 cut(s) 498
BstX2I RGATCY 2 cut(s) 110, 811
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 2 cut(s) 110, 811
BsuI GTATCC 1 cut(s) 603
BtgI CCRYGG 1 cut(s) 764
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 2 cut(s) 631, 834
BtsIMutI CAGTG 2 cut(s) 131, 297
Cac8I GCNNGC 1 cut(s) 761
CfoI GCGC 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 25
CseI GACGC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 352
CviAII CATG 2 cut(s) 443, 670
CviJI RGCY 7 cut(s) 272, 491, 497, 579, 759, 908, 926
CviKI_1 RGCY 7 cut(s) 272, 491, 497, 579, 759, 908, 926
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 5 cut(s) 550, 561, 606, 722, 927
DpnI GATC 5 cut(s) 112, 137, 224, 813, 833
DpnII GATC 5 cut(s) 110, 135, 222, 811, 831
DriI GACNNNNNGTC 1 cut(s) 31
Eam1104I CTCTTC 3 cut(s) 127, 133, 250
Eam1105I GACNNNNNGTC 1 cut(s) 31
EarI CTCTTC 3 cut(s) 127, 133, 250
EciI GGCGGA 1 cut(s) 339
Eco31I GGTCTC 3 cut(s) 717, 745, 870
Eco32I GATATC 1 cut(s) 527
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 477
Eco88I CYCGRG 1 cut(s) 190
EcoRI GAATTC 1 cut(s) 673
EcoRV GATATC 1 cut(s) 527
FaeI CATG 2 cut(s) 446, 673
FaiI YATR 6 cut(s) 345, 444, 538, 651, 671, 696
FatI CATG 2 cut(s) 442, 669
FbaI TGATCA 1 cut(s) 831
FblI GTMKAC 1 cut(s) 800
Fnu4HI GCNGC 2 cut(s) 6, 489
FokI GGATG 2 cut(s) 638, 821
Fsp4HI GCNGC 2 cut(s) 6, 489
FspBI CTAG 4 cut(s) 210, 363, 642, 717
GlaI GCGC 1 cut(s) 9
GluI GCNGC 2 cut(s) 6, 489
GsuI CTGGAG 1 cut(s) 930
HapII CCGG 2 cut(s) 108, 576
HgaI GACGC 1 cut(s) 311
HhaI GCGC 1 cut(s) 10
Hin1II CATG 2 cut(s) 446, 673
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HinfI GANTC 8 cut(s) 68, 89, 258, 373, 381, 453, 614, 940
HpaII CCGG 2 cut(s) 108, 576
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 3 cut(s) 185, 521, 801
Hpy188I TCNGA 6 cut(s) 79, 250, 597, 690, 839, 858
Hpy188III TCNNGA 9 cut(s) 175, 192, 262, 363, 407, 750, 819, 937, 964
Hpy8I GTNNAC 3 cut(s) 185, 521, 801
Hpy99I CGWCG 4 cut(s) 83, 170, 223, 268
HpyAV CCTTC 5 cut(s) 143, 149, 477, 509, 825
HpyCH4IV ACGT 3 cut(s) 32, 168, 179
HpyF10VI GCNNNNNNNGC 1 cut(s) 494
HpyF3I CTNAG 5 cut(s) 550, 561, 606, 722, 927
HpySE526I ACGT 3 cut(s) 32, 168, 179
Hsp92II CATG 2 cut(s) 446, 673
HspAI GCGC 1 cut(s) 8
Ksp22I TGATCA 1 cut(s) 831
Kzo9I GATC 5 cut(s) 110, 135, 222, 811, 831
LpnPI CCDG 7 cut(s) 121, 171, 342, 573, 589, 603, 894
LweI GCATC 2 cut(s) 73, 319
MaeI CTAG 4 cut(s) 210, 363, 642, 717
MaeII ACGT 3 cut(s) 32, 168, 179
MaeIII GTNAC 4 cut(s) 37, 226, 665, 961
MalI GATC 5 cut(s) 112, 137, 224, 813, 833
MboI GATC 5 cut(s) 110, 135, 222, 811, 831
MfeI CAATTG 1 cut(s) 881
MflI RGATCY 2 cut(s) 110, 811
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 6 cut(s) 18, 419, 447, 673, 881, 967
MmeI TCCRAC 2 cut(s) 102, 394
MnlI CCTC 5 cut(s) 16, 136, 248, 306, 728
MseI TTAA 1 cut(s) 970
MspI CCGG 2 cut(s) 108, 576
MunI CAATTG 1 cut(s) 881
MwoI GCNNNNNNNGC 1 cut(s) 494
NdeII GATC 5 cut(s) 110, 135, 222, 811, 831
NlaIII CATG 2 cut(s) 446, 673
NlaIV GGNNCC 2 cut(s) 465, 813
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 2 cut(s) 37, 961
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PfeI GAWTC 8 cut(s) 68, 89, 258, 373, 381, 453, 614, 940
PkrI GCNGC 2 cut(s) 7, 490
Ple19I CGATCG 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 465, 813
PspPI GGNCC 1 cut(s) 25
PsuI RGATCY 2 cut(s) 110, 811
PvuI CGATCG 1 cut(s) 225
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
SaqAI TTAA 1 cut(s) 970
SatI GCNGC 2 cut(s) 6, 489
Sau3AI GATC 5 cut(s) 110, 135, 222, 811, 831
Sau96I GGNCC 1 cut(s) 25
SduI GDGCHC 1 cut(s) 165
SfaNI GCATC 2 cut(s) 73, 319
SfcI CTRYAG 1 cut(s) 498
SinI GGWCC 1 cut(s) 25
Sse9I AATT 6 cut(s) 18, 419, 447, 673, 881, 967
SsiI CCGC 3 cut(s) 5, 324, 488
SspI AATATT 1 cut(s) 63
SspMI CTAG 4 cut(s) 210, 363, 642, 717
TaiI ACGT 3 cut(s) 35, 171, 182
TaqI TCGA 3 cut(s) 15, 66, 749
TasI AATT 6 cut(s) 18, 419, 447, 673, 881, 967
TauI GCSGC 2 cut(s) 8, 491
TfiI GAWTC 8 cut(s) 68, 89, 258, 373, 381, 453, 614, 940
Tru1I TTAA 1 cut(s) 970
Tru9I TTAA 1 cut(s) 970
TscAI CASTG 2 cut(s) 138, 304
TseFI GTSAC 2 cut(s) 37, 961
Tsp45I GTSAC 2 cut(s) 37, 961
TspDTI ATGAA 3 cut(s) 525, 686, 839
TspGWI ACGGA 3 cut(s) 25, 408, 877
TspRI CASTG 2 cut(s) 138, 304
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 2 cut(s) 419, 673
XbaI TCTAGA 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 762
XmiI GTMKAC 1 cut(s) 800
XspI CTAG 4 cut(s) 210, 363, 642, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.