AT1G70440

Encodes a protein with similarity to RCD1 but without the WWE domain. The protein does have a PARP signature upstream of the C-terminal protein interaction domain. The PARP signature may bind NAD and attach the ADP-ribose-moiety from NAD to the target molecule. Its presence suggests a role for the protein in ADP ribosylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010041
Physical Location & Seq
Reverse (-)
26548952 .. 26550636
1685 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70440.1

Sequence Viewer

Length: 918 bp
ATGGCGGCACAAGTCGAGATCGAAGATCAAGAATCCGTCACGAATTTAGACAACGGCGAAATCATCAACCCCATCTCCGACAACGCACCTAACTTCTCCGGCGATGCAACGATTCTTCTCCGTGAAGCCACCTTCGAACACAACCTCATCAAAAACTGTTTCCTCTCCGGAATGGGCTCATTCGCCACCGAAACAACAATCGTCACCGTACGAAAAATCTTAACGCAGAGATTAATCACAACGAAAGCTAAATTCGCTGTGTTCAAGCTTTTCACAGAAGCTATGAAGAGGAAGAACAACGGTTACGCGAATATTAGGTACGGTTGGTACTCTGGTTCTAAAGAAGAGATCGATCGTGTTATTACGTATGGGTTTAGTAATAGAGAGATCAAGAAAGTCGAAAACGACGTCGGATCTCACGGTGTTGGAATTCATCTTGTTCATCATCGTTACTCTCTCGCTGCGGCTTTAGTTGGTGAAGGTGATGAAGAAGGGATCAAGAATATTCTCTTATGCCGTGTGATTCTTGGTAAACCGGAACAAATTGTGACCGGTTCTAAACAATCTTACCCAAGTTCAAACCAATTTGATTCTGGTGTTGATAATCTTGAGAATCCGAGAAAATATGTGATTTGGAGTTGTAACATGAATTCTTACATTCTTCCAACTTATATCGTCAGTTTCAAATCTCATCTTCTCAGAGGTCTAATTGGAAGAGCAAGGTCACCATGTGTTAGCTTCTCTGTTCTTATGTCAATTCTTTCAAAGTCACTTGACGCTGCAAGAATGAACTTGATTTTGACTAGTTATGATGATTTTAGGAAACGGAAACTGAGGAGAGAGCAGCTAGTTAGGAAGATTAGGGAAGTGGTTGGAGACAATCTTCTTTTCAAGATACTCAAGAATCAGAGGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

305

Amino Acids

34.5

Weight (kDa)

9.46

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 241 - 303 2.1e-21 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23550 AT1G70440 AT1G70440
fragaria_vesca FvH4_4g34350
malus_domestica MD13G1092800.v1.1 MD16G1093700.v1.1
prunus_persica Prupe.1G252800_v2.0.a1
pyrus_communis pycom13g08070 pycom13g08080 pycom16g07970
rosa_chinensis RchiOBHm_Chr4g0443761
rosa_laevigata RLG00000005892
rosa_multiflora Rmu_co8242545.1_g000001 Rmu_sc0042927.1_g000001
rosa_roxburghii Rroxscaffold_5G00384330
rosa_rugosa Rorug04G0347100
rosa_samantha Rh4AG407300 Rh4BG418200 Rh4CG432500 Rh4DG413500
rosa_wichuraiana Rw4G034980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 411
AccII CGCG 1 cut(s) 308
AccIII TCCGGA 1 cut(s) 167
AciI CCGC 2 cut(s) 5, 464
AclWI GGATC 2 cut(s) 421, 503
AcsI RAATTY 4 cut(s) 43, 251, 429, 649
AcyI GRCGYC 1 cut(s) 408
AdeI CACNNNGTG 1 cut(s) 731
AfaI GTAC 3 cut(s) 210, 320, 329
AgeI ACCGGT 1 cut(s) 551
AgsI TTSAA 5 cut(s) 265, 579, 685, 765, 892
AhlI ACTAGT 1 cut(s) 803
AluBI AGCT 5 cut(s) 248, 268, 281, 738, 847
AluI AGCT 5 cut(s) 248, 268, 281, 738, 847
Alw26I GTCTC 1 cut(s) 870
AlwI GGATC 2 cut(s) 421, 503
Aor13HI TCCGGA 1 cut(s) 167
ApeKI GCWGC 3 cut(s) 461, 779, 844
ApoI RAATTY 4 cut(s) 43, 251, 429, 649
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
AseI ATTAAT 1 cut(s) 233
AsiGI ACCGGT 1 cut(s) 551
AsuHPI GGTGA 4 cut(s) 196, 488, 494, 717
AsuII TTCGAA 1 cut(s) 135
BaeI ACNNNNGTAYC 2 cut(s) 319, 352
BanII GRGCYC 1 cut(s) 179
BbvI GCAGC 3 cut(s) 448, 766, 856
BccI CCATC 1 cut(s) 80
BceAI ACGGC 2 cut(s) 70, 501
BcoDI GTCTC 1 cut(s) 870
BcuI ACTAGT 1 cut(s) 803
BfaI CTAG 2 cut(s) 804, 848
BisI GCNGC 5 cut(s) 6, 462, 465, 780, 845
BlsI GCNGC 5 cut(s) 7, 463, 466, 781, 846
BmsI GCATC 1 cut(s) 94
Bpu14I TTCGAA 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 629, 884
Bsa29I ATCGAT 1 cut(s) 351
BsaAI YACGTR 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 408
BsaWI WCCGGW 3 cut(s) 167, 535, 551
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bse118I RCCGGY 1 cut(s) 551
BseAI TCCGGA 1 cut(s) 167
BseCI ATCGAT 1 cut(s) 351
BseMII CTCAG 2 cut(s) 712, 824
BseRI GAGGAG 1 cut(s) 850
BseXI GCAGC 3 cut(s) 448, 766, 856
Bsh1236I CGCG 1 cut(s) 308
Bsh1285I CGRYCG 1 cut(s) 355
BshTI ACCGGT 1 cut(s) 551
BshVI ATCGAT 1 cut(s) 351
BsiEI CGRYCG 1 cut(s) 355
BsiSI CCGG 4 cut(s) 99, 168, 536, 552
BsiWI CGTACG 1 cut(s) 208
BsmAI GTCTC 1 cut(s) 870
Bsp119I TTCGAA 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 179
Bsp13I TCCGGA 1 cut(s) 167
Bsp143I GATC 7 cut(s) 18, 25, 348, 352, 387, 413, 495
BspACI CCGC 2 cut(s) 5, 464
BspCNI CTCAG 2 cut(s) 711, 825
BspDI ATCGAT 1 cut(s) 351
BspEI TCCGGA 1 cut(s) 167
BspFNI CGCG 1 cut(s) 308
BspPI GGATC 2 cut(s) 421, 503
BspQI GCTCTTC 1 cut(s) 709
BspT104I TTCGAA 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 551
BssAI RCCGGY 1 cut(s) 551
BssMI GATC 7 cut(s) 18, 25, 348, 352, 387, 413, 495
BssNI GRCGYC 1 cut(s) 408
Bst4CI ACNGT 5 cut(s) 158, 208, 302, 323, 422
Bst6I CTCTTC 3 cut(s) 281, 339, 709
BstACI GRCGYC 1 cut(s) 408
BstBAI YACGTR 1 cut(s) 366
BstBI TTCGAA 1 cut(s) 135
BstDEI CTNAG 2 cut(s) 698, 833
BstEII GGTNACC 1 cut(s) 723
BstFNI CGCG 1 cut(s) 308
BstKTI GATC 7 cut(s) 21, 28, 351, 355, 390, 416, 498
BstMAI GTCTC 1 cut(s) 870
BstMBI GATC 7 cut(s) 18, 25, 348, 352, 387, 413, 495
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstPI GGTNACC 1 cut(s) 723
BstSNI TACGTA 1 cut(s) 366
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 3 cut(s) 448, 766, 856
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
Bsu15I ATCGAT 1 cut(s) 351
BsuTUI ATCGAT 1 cut(s) 351
BtgZI GCGATG 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 551
ClaI ATCGAT 1 cut(s) 351
CseI GACGC 1 cut(s) 785
Csp6I GTAC 3 cut(s) 209, 319, 328
CspAI ACCGGT 1 cut(s) 551
CviAII CATG 2 cut(s) 646, 729
CviJI RGCY 8 cut(s) 128, 177, 248, 268, 281, 467, 738, 847
CviKI_1 RGCY 8 cut(s) 128, 177, 248, 268, 281, 467, 738, 847
CviQI GTAC 3 cut(s) 209, 319, 328
DdeI CTNAG 2 cut(s) 698, 833
DpnI GATC 7 cut(s) 20, 27, 350, 354, 389, 415, 497
DpnII GATC 7 cut(s) 18, 25, 348, 352, 387, 413, 495
DraIII CACNNNGTG 1 cut(s) 731
Eam1104I CTCTTC 3 cut(s) 281, 339, 709
EarI CTCTTC 3 cut(s) 281, 339, 709
Eco105I TACGTA 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 179
Eco91I GGTNACC 1 cut(s) 723
EcoO65I GGTNACC 1 cut(s) 723
EcoRI GAATTC 2 cut(s) 429, 649
EcoT38I GRGCYC 1 cut(s) 179
FaeI CATG 2 cut(s) 649, 732
FaiI YATR 9 cut(s) 284, 369, 514, 627, 647, 672, 730, 752, 810
FatI CATG 2 cut(s) 645, 728
Fnu4HI GCNGC 5 cut(s) 6, 462, 465, 780, 845
FriOI GRGCYC 1 cut(s) 179
Fsp4HI GCNGC 5 cut(s) 6, 462, 465, 780, 845
FspBI CTAG 2 cut(s) 804, 848
GluI GCNGC 5 cut(s) 6, 462, 465, 780, 845
HapII CCGG 4 cut(s) 99, 168, 536, 552
HgaI GACGC 1 cut(s) 785
Hin1I GRCGYC 1 cut(s) 408
Hin1II CATG 2 cut(s) 649, 732
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 6 cut(s) 32, 112, 523, 590, 613, 904
HpaII CCGG 4 cut(s) 99, 168, 536, 552
HphI GGTGA 4 cut(s) 196, 488, 494, 717
Hpy166II GTNNAC 1 cut(s) 533
Hpy188I TCNGA 5 cut(s) 79, 413, 618, 701, 909
Hpy188III TCNNGA 9 cut(s) 16, 29, 40, 168, 391, 499, 608, 892, 901
Hpy8I GTNNAC 1 cut(s) 533
Hpy99I CGWCG 2 cut(s) 410, 413
HpyAV CCTTC 3 cut(s) 142, 473, 485
HpyCH4III ACNGT 5 cut(s) 158, 208, 302, 323, 422
HpyCH4IV ACGT 2 cut(s) 365, 408
HpyCH4V TGCA 2 cut(s) 107, 782
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 2 cut(s) 698, 833
HpySE526I ACGT 2 cut(s) 365, 408
Hsp92I GRCGYC 1 cut(s) 408
Hsp92II CATG 2 cut(s) 649, 732
Kpn2I TCCGGA 1 cut(s) 167
Kzo9I GATC 7 cut(s) 18, 25, 348, 352, 387, 413, 495
LguI GCTCTTC 1 cut(s) 709
LpnPI CCDG 6 cut(s) 112, 181, 318, 549, 565, 579
Lsp1109I GCAGC 3 cut(s) 448, 766, 856
LweI GCATC 1 cut(s) 94
MaeI CTAG 2 cut(s) 804, 848
MaeII ACGT 2 cut(s) 365, 408
MaeIII GTNAC 8 cut(s) 37, 202, 302, 449, 547, 641, 723, 768
MalI GATC 7 cut(s) 20, 27, 350, 354, 389, 415, 497
MboI GATC 7 cut(s) 18, 25, 348, 352, 387, 413, 495
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 8 cut(s) 43, 251, 429, 543, 584, 649, 708, 756
MmeI TCCRAC 5 cut(s) 102, 391, 406, 689, 853
MnlI CCTC 6 cut(s) 155, 173, 282, 695, 828, 903
MroI TCCGGA 1 cut(s) 167
MseI TTAA 3 cut(s) 221, 233, 916
MspI CCGG 4 cut(s) 99, 168, 536, 552
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeII GATC 7 cut(s) 18, 25, 348, 352, 387, 413, 495
NlaIII CATG 2 cut(s) 649, 732
NmuCI GTSAC 5 cut(s) 37, 202, 547, 723, 768
NspV TTCGAA 1 cut(s) 135
PciSI GCTCTTC 1 cut(s) 709
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PfeI GAWTC 6 cut(s) 32, 112, 523, 590, 613, 904
Pfl23II CGTACG 1 cut(s) 208
PinAI ACCGGT 1 cut(s) 551
PkrI GCNGC 5 cut(s) 7, 463, 466, 781, 846
Ple19I CGATCG 1 cut(s) 355
Ppu21I YACGTR 1 cut(s) 366
PshBI ATTAAT 1 cut(s) 233
PspEI GGTNACC 1 cut(s) 723
PspLI CGTACG 1 cut(s) 208
PsrI GAACNNNNNNTAC 2 cut(s) 287, 319
PsuI RGATCY 1 cut(s) 413
PvuI CGATCG 1 cut(s) 355
RsaI GTAC 3 cut(s) 210, 320, 329
RsaNI GTAC 3 cut(s) 209, 319, 328
SapI GCTCTTC 1 cut(s) 709
SaqAI TTAA 3 cut(s) 221, 233, 916
SatI GCNGC 5 cut(s) 6, 462, 465, 780, 845
Sau3AI GATC 7 cut(s) 18, 25, 348, 352, 387, 413, 495
SduI GDGCHC 1 cut(s) 179
SfaNI GCATC 1 cut(s) 94
SfuI TTCGAA 1 cut(s) 135
SmlI CTYRAG 2 cut(s) 608, 899
SmoI CTYRAG 2 cut(s) 608, 899
SnaBI TACGTA 1 cut(s) 366
SpeI ACTAGT 1 cut(s) 803
Sse9I AATT 8 cut(s) 43, 251, 429, 543, 584, 649, 708, 756
SsiI CCGC 2 cut(s) 5, 464
SspI AATATT 2 cut(s) 313, 505
SspMI CTAG 2 cut(s) 804, 848
TaaI ACNGT 5 cut(s) 158, 208, 302, 323, 422
TaiI ACGT 2 cut(s) 368, 411
TaqI TCGA 5 cut(s) 15, 21, 135, 351, 399
TasI AATT 8 cut(s) 43, 251, 429, 543, 584, 649, 708, 756
TauI GCSGC 2 cut(s) 8, 467
TfiI GAWTC 6 cut(s) 32, 112, 523, 590, 613, 904
Tru1I TTAA 3 cut(s) 221, 233, 916
Tru9I TTAA 3 cut(s) 221, 233, 916
TseFI GTSAC 5 cut(s) 37, 202, 547, 723, 768
TseI GCWGC 3 cut(s) 461, 779, 844
Tsp45I GTSAC 5 cut(s) 37, 202, 547, 723, 768
TspDTI ATGAA 6 cut(s) 299, 422, 431, 501, 662, 803
TspGWI ACGGA 3 cut(s) 25, 110, 841
VspI ATTAAT 1 cut(s) 233
XapI RAATTY 4 cut(s) 43, 251, 429, 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 590
XspI CTAG 2 cut(s) 804, 848
ZraI GACGTC 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.