AT1G24300

GYF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005720
Physical Location & Seq
Reverse (-)
8614363 .. 8620664
6302 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G24300.3

Sequence Viewer

Length: 4488 bp
ATGGCCGAAGGGAAGTTCGATCTGCCTGACGATCTCATCCTTTCCAAATCTTCTGATCAGTTGAAAGAATTGGCCTCAGATAACAGCATTCCGTTGTCTCCGCAGTGGCTTTACACGAAATCAAGCGAGAGCAAGATGGATGTTCGATCTCCTACTCCTATGCCCATGGGAAATCCAAGTGACCCAAATCTGAAGGATGCATGGCGCTTGGATGCACCCGAAGACAAAAAAGACTGGAAGAAAATTGTGTCTGAGAATGAAACTAATCGCAGATGGCGTGAAGAGGAAAGAGAAACTGGTTTACTCGGTGCTAGGAAAGTTGATCGACGAAAAACAGAGCGTCGCATTGACAATGTCTCAAGCAGAGAAACTGGTGAAGTTAAAACTACTGCTGCTTCTGATAGGTGGAATGATGTTAATTCTCGTGCTGCTGTACATGAACCCCGTCGCGACAACAAATGGTCATCTCGGTGGGGTCCTGATGACAAAGAGAAAGAAGCCCGTTGTGAGAAGGTAGAGATTAACAAGGACAAGGAAGAGCCTCAGAGTGAAAGTCAATCTGTTGTTAGCAATGTCCGTGCAACTTCTGAACGGGACTCTGACCCTCGTGACAAATGGAGGCCACGTCATAGGATGGAATCGCAGTCTGGTGTGCCTACTTCTTATCGTACTGCACCTGGGTTTGGACTTGATAGAGGACGAGCTGAGGGCCCAAATTTAGGGTTCACTGTTGGCCGAGGAAGGGCATCTACTATTGGGAGGGGTTCTTCAACTTCCTTAATTGGTGCTGGTAGTGCTTCAGCCCCTGTGTTTCGGTATCCAAGGGGTAAGTTGCTTGATATGTATAGAAAACAGAAGCCTGATCCTTCCCTAGGTAGGATACCGACTGAAATGGATGAAGTTGCCTCCATAACGCAAGTGGCTCTGATCGAACCTCTTGCTTTTATCGCACCTGATACCGAAGAAGAGGCAAGCATTAACGGCATATGGAAGGGAAGGATCATTAGTAGTGAAGTTTACACTTCATCTGGGGAAGAGTCCTTAGGTGAAAACAGTCTGTTGAAATGCCGCATACCTGAGTCAGGTGAGACAAAAGTGGATGGTGCTTTGCTAGGGTTTATGAATGGTGACAATGGCTCCATGAAAAATAATGATTCCGGATTACTTGGTAGTCATAATGGAGGTCTGGGGGCAGCTTCTAGCGTCCCAAGATTGAATTCAGTGGCTTCTGAAAGTTATGGATCTTTTGGAGCTGGTTATCAAGTTTCTCATGGAAGTCCTGAAGCAGTTAGATCAGTTTTTACTAAGTCCCCTGTGTTGGATGGGAGCGAATCTGTTGTTGGGTCGTTCGAGCAAGATTATATGGGCAAGCTGCAGCAACCAGATGTTGAAGTGGATCAGTCAGAAGCGGCTATGCCACCTGAGGATTTCTTATTCTTGTATATTGATCCTCAAGGAGTAATTCAGGGACCATTCATTGGTTCTGACATTATTTCATGGTTTGAACAAGGGTTTTTTGGGACAGACCTACAGGTGCGCTTGGCAAATGCTCCAGAAGGAACTCCTTTTCAAGATCTAGGCAGGGTCATGTCATATTTAAAGACAGAAAGCGCCCATGCCCATATCAGTAACCAAGAGAGTGAGCTAGAAGAGACTAGGTTAAAAGCAAATTCAGACACTGGTTTATCAATTGCACCAGTAGCAGAATCTAATGATTCATCCTCTATGAATGGCACGTCTCGTTCGTTTTCAGTGTATAACAACCCCTCAGCTCAGGATAATTTTCAAAGAAAGTCTGAGTCAGAATTTTATGCGACACCACCACATACTGAGGACCGAAGTTTCTTGGACTTCTCTACTCAGGATGAAGAAATTGTATTCCCAGGAAGAGCTGGAGTTTCTGGTTATGCGTCAGTAAAATCCTCTACAAGTATGCATGATGCTTTTATGGAAGTTTCTGGCCAGTCTGCTATTCCTGTTGAATCCACTAAGGCTGCTACTCAAAAGCAGCATGAGAATAAGCTGCATCCGTTTGGTGTGTTGTGGTCTGAGCTAGAGAGCAGTAACGTACCAGTTAACCTGTTACCAAACAGGTCCTATGATGCAATGGGGGAGCCTACTGGTGCAATAGACAATCGGCCCATTGATTCCCGAAGAAATACACAAGTTGATCCCAACATGTCCCTTGATGGTCTGGCTTCTAACCGGATGTCGCAGTTTGAACATCTTTCCAACCGCTTTAACCTTGGGGATCAACTTTCATCAAATCAACATAACCAACAGCAATTCCAAAACCGGGATATGCTGTCGCATTCACATATAGGTGATCAAGCTCAGGATCTGGATTATTTAATAACTCTCCAGTTGCAGCAGCAACAAAAGATTCAGTTGCAACAGCAGCAGAAGATTCAATTGCAGCAGCAGCAGAAGATTCAGTTGCAACAGCAGCAGAAGATGCAGCTGCAACAGCATCAACTAGAACAAGAGCATCAGTTACATCAGAAGCTCTTACAGGAGCAACAACAGTCCCATGCTCGGCAATTACATTATCAACAGATTCTACAAGGACAGACTCCTGATTCAAGGTTTGGGCAGTCACATGACTTCCCTCGATCCAACAATGTTGATCAGATGTTGTTAGAACACCAGCTAATGAATGAACTGCAGAAAAGTTCTGGCCATCCGTCACAAAATTTTGCACCATACATTGAGCAACTTGCCGCTGGAAATTTTGGTCAATTGCCACATGAAGGCCATCAGAGGGAACTACTTGAACAGCTACTTTCAACAAAAATGCAATCTCAATATGGACCAATGCAATCTCCATACGGACAACTGCAATCTGAACCAACTCGGTCTTTGGAGTACCAACTGCTGCAGCAAGAGCAGCTGATGCAATTGGCAAACGGAGTGAGGCACAATACACTATTGGAGGAACAGAGACATATTGACCCTCTGTGGCCATCTGACCATAATGATCAGCTTCTAAGAAGTCATCCTGGGATCCAACGTTCGCGATCCTCTACAGGATTTAGACAACTAGACTTTCATCAACAGCAGCAGAGGCCACCCTTCGAGGATCAGTTTGGTCAACTTGAGCGGAACCTTTTATATCAGCAACAGCTTCGACAAGAATTATTCGAGCAGGGTCTGCCATTTGAGCGATCAGCATCCTTACCTGTAAGTGTATCGGGGATGAATCTGGATCCAGTAAATGGTCTTGGACTCTCTCAAGGTCTGGAGTTGCGGGATGCTACTACCCACATGCAGATTGGGAATTCTACTCTGGGTTTTAATCATCAGAATCCCCGTATACCAATAGGTGAACCTCATTTTTCACAGTTGGAATCAATGGAAGGACGTTGGTCTGGAGCTGACACACAGGTTGTTGGTGACTGGGCCGAATCTCAACTTCATAGATCAAATATTGATGCTGAACATCATAAAATGAGGTCAGAAAGTAGGAGAATGGGTGAAGATTCCAACTCATGGATGTTAGGTGGGACTACAGAAGACAGGTCAAAACAGCTATTTATGGAGTTGCTCCACCAGAGACCCGGCCATCAATCTGCTGAATCACCAAGCATGAACCGTGGGCAATCCTTTGACAGGATGGCTCCTTCTGGCCTTACCCCAGGGATTCAAACACTTGGCGGCTATTCAGATCATGGTGGTAGTCATAACGCTCCTTCAACTTTTGGTGCTCGAGCATTTTCTGATGAACAGATCAACAGATCATCCGGAGATAGGAACAATATGGGATCATTGCACCGTAATAATTCCTTGCTTTCTGGAATTATTGATGGTGGGCGGTCAACTCAGAATGAAACTCAAGCCTTCAGTAATATGTATGCTATGAACAAAGACGCGAATGACATCAAAACTTGGAACAATGTGCCGCCTAAAAATGAGGGAATGGGAAGGATGATGTCATTTGAAGCCCAAGATAGAATGGGCAAGCAAGCAGTATTAGACTCACTGGTTCATGGGGAGCTTCCTGTTGTTACGCCTGGCCAACAGTCTTCTTTAAATATCTCTGACCAATACAGTGACAACTTGGTTGGAGAAGATAGAAGGAAGGATAGGTTGGTAGTTCCATCACATGGACAGAATTCAGTATTGTTAAAAAGGCCTCCCTCATCCCATTCTTCATCATCGCATGAAGGATTGATAGAGCGTATCTCTGACACAGCTAGCAGGACAGCAGCAAGTTCGTATAGTGGAATTGAAGGCGGTGTGAGACGGGAATCAGGAGCAGCGGGAAACAAAGGGTCAACGTCAGAGGCGACATCGTTCAGTGAGATGTTGAAGAAGAGTAATAGCATGAAGAAGGTGGCGGCAGAGTCAAATGATGTAACAGAAGGAAGCAAAGGTGGTGGCGGGAAGAAGAAAGGGAAGAAAGGGAGGCAGATCGATCCAGCTCTTCTTGGTTTCAAAGTCACTAGCAATCGCATTATGGGCGAGATCCACCGTGCTGATGATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1495

Amino Acids

166.27

Weight (kDa)

5.68

Isoelectric Point (pI)

59.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 480 - 524 3.4e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 4 cut(s) 1478, 3215, 3489, 4103
AccBSI CCGCTC 1 cut(s) 3100
AccI GTMKAC 1 cut(s) 3313
AccII CGCG 3 cut(s) 450, 3016, 3869
AccIII TCCGGA 2 cut(s) 1157, 3740
AclI AACGTT 1 cut(s) 3010
AcoI YGGCCR 7 cut(s) 3, 733, 1960, 2677, 2960, 3559, 4012
AcsI RAATTY 8 cut(s) 715, 1216, 1669, 1805, 2692, 2728, 3277, 4111
AcuI CTGAAG 4 cut(s) 212, 783, 1302, 3823
AfaI GTAC 4 cut(s) 435, 670, 2070, 2867
AflIII ACRYGT 1 cut(s) 2178
AjiI CACGTC 2 cut(s) 626, 1737
AjnI CCWGG 5 cut(s) 676, 1882, 2998, 3634, 4009
AjuI GAANNNNNNNTTGG 6 cut(s) 1201, 1233, 1323, 1355, 4070, 4102
AloI GAACNNNNNNTCC 2 cut(s) 2995, 3027
Alw21I GWGCWC 1 cut(s) 3706
Alw26I GTCTC 8 cut(s) 102, 361, 1082, 1646, 1743, 2935, 3547, 4234
AlwNI CAGNNNCTG 4 cut(s) 806, 1679, 2341, 3151
Ama87I CYCGRG 1 cut(s) 3705
Aor13HI TCCGGA 2 cut(s) 1157, 3740
ApaI GGGCCC 1 cut(s) 713
ApoI RAATTY 8 cut(s) 715, 1216, 1669, 1805, 2692, 2728, 3277, 4111
AspA2I CCTAGG 1 cut(s) 871
AspLEI GCGC 3 cut(s) 207, 1539, 1613
AspS9I GGNCC 9 cut(s) 476, 709, 710, 1469, 1834, 2094, 2139, 2810, 3399
AsuC2I CCSGG 2 cut(s) 2297, 3558
AsuHPI GGTGA 9 cut(s) 386, 1058, 1097, 1139, 2336, 3335, 3404, 3485, 3570
AsuNHI GCTAGC 1 cut(s) 4193
AvaI CYCGRG 1 cut(s) 3705
AvaII GGWCC 5 cut(s) 476, 1469, 1834, 2094, 2810
AvrII CCTAGG 1 cut(s) 871
AxyI CCTNAGG 2 cut(s) 1042, 1422
BaeGI GKGCMC 1 cut(s) 713
BalI TGGCCA 4 cut(s) 1962, 2679, 2962, 4014
BamHI GGATCC 2 cut(s) 3003, 3205
BanII GRGCYC 1 cut(s) 713
BarI GAAGNNNNNNTAC 2 cut(s) 733, 765
BauI CACGAG 2 cut(s) 423, 606
BbsI GAAGAC 3 cut(s) 228, 3519, 4014
Bbv12I GWGCWC 1 cut(s) 3706
BbvCI CCTCAGC 2 cut(s) 705, 1768
BceAI ACGGC 1 cut(s) 997
BcgI CGANNNNNNTGC 6 cut(s) 920, 954, 3107, 3141, 4194, 4228
BciT130I CCWGG 5 cut(s) 678, 1884, 3000, 3636, 4011
BciVI GTATCC 2 cut(s) 828, 873
BclI TGATCA 4 cut(s) 55, 2326, 2626, 2977
BcnI CCSGG 2 cut(s) 2297, 3558
BcoDI GTCTC 8 cut(s) 102, 361, 1082, 1646, 1743, 2935, 3547, 4234
BfmI CTRYAG 6 cut(s) 1373, 1529, 2663, 2876, 3024, 3507
BfoI RGCGCY 2 cut(s) 208, 1614
BfuI GTATCC 2 cut(s) 828, 873
BglII AGATCT 1 cut(s) 1573
BlnI CCTAGG 1 cut(s) 871
Bme1390I CCNGG 7 cut(s) 678, 1884, 2297, 3000, 3558, 3636, 4011
Bme18I GGWCC 5 cut(s) 476, 1469, 1834, 2094, 2810
BmeT110I CYCGRG 1 cut(s) 3705
BmgBI CACGTC 2 cut(s) 626, 1737
BmgT120I GGNCC 9 cut(s) 476, 709, 710, 1469, 1834, 2094, 2139, 2810, 3399
BmiI GGNNCC 9 cut(s) 477, 711, 1138, 1470, 2115, 3005, 3104, 3207, 3618
BmrFI CCNGG 7 cut(s) 678, 1884, 2297, 3000, 3558, 3636, 4011
BmrI ACTGGG 1 cut(s) 3406
BmtI GCTAGC 1 cut(s) 4197
BmuI ACTGGG 1 cut(s) 3406
BoxI GACNNNNGTC 1 cut(s) 3364
BpiI GAAGAC 3 cut(s) 228, 3519, 4014
BplI GAGNNNNNCTC 2 cut(s) 4166, 4198
BpmI CTGGAG 5 cut(s) 1536, 1914, 2345, 3260, 3390
Bpu10I CCTNAGC 4 cut(s) 705, 1768, 1773, 2334
BpuEI CTTGAG 5 cut(s) 343, 1437, 3116, 3216, 3816
BpuMI CCSGG 2 cut(s) 2297, 3558
Bsa29I ATCGAT 1 cut(s) 4413
BsaBI GATNNNNATC 1 cut(s) 3169
BsaI GGTCTC 1 cut(s) 3547
BsaWI WCCGGW 3 cut(s) 1157, 2205, 3740
BsaXI ACNNNNNCTCC 6 cut(s) 610, 640, 1121, 1151, 3457, 3487
Bse21I CCTNAGG 2 cut(s) 1042, 1422
Bse3DI GCAATG 3 cut(s) 577, 2112, 3764
Bse8I GATNNNNATC 1 cut(s) 3169
BseAI TCCGGA 2 cut(s) 1157, 3740
BseBI CCWGG 5 cut(s) 678, 1884, 3000, 3636, 4011
BseCI ATCGAT 1 cut(s) 4413
BseJI GATNNNNATC 1 cut(s) 3169
BseMI GCAATG 3 cut(s) 577, 2112, 3764
BseSI GKGCMC 1 cut(s) 713
BsgI GTGCAG 1 cut(s) 657
Bsh1236I CGCG 3 cut(s) 450, 3016, 3869
BshVI ATCGAT 1 cut(s) 4413
BsiHKAI GWGCWC 1 cut(s) 3706
BsiHKCI CYCGRG 1 cut(s) 3705
BsiSI CCGG 5 cut(s) 1158, 2206, 2296, 3558, 3741
BslFI GGGAC 8 cut(s) 608, 1190, 1294, 1482, 1534, 2167, 2512, 3517
BsmAI GTCTC 8 cut(s) 102, 361, 1082, 1646, 1743, 2935, 3547, 4234
BsmBI CGTCTC 2 cut(s) 1743, 4234
BsmFI GGGAC 8 cut(s) 608, 1190, 1294, 1482, 1534, 2167, 2512, 3517
BsmI GAATGC 2 cut(s) 87, 2311
Bso31I GGTCTC 1 cut(s) 3547
BsoBI CYCGRG 1 cut(s) 3705
Bsp120I GGGCCC 1 cut(s) 709
Bsp1286I GDGCHC 2 cut(s) 713, 3706
Bsp13I TCCGGA 2 cut(s) 1157, 3740
Bsp1407I TGTACA 1 cut(s) 433
Bsp19I CCATGG 1 cut(s) 165
Bsp68I TCGCGA 2 cut(s) 450, 3016
BspDI ATCGAT 1 cut(s) 4413
BspEI TCCGGA 2 cut(s) 1157, 3740
BspFNI CGCG 3 cut(s) 450, 3016, 3869
BspLI GGNNCC 9 cut(s) 477, 711, 1138, 1470, 2115, 3005, 3104, 3207, 3618
BspMAI CTGCAG 3 cut(s) 1377, 2667, 2880
BspOI GCTAGC 1 cut(s) 4197
BspQI GCTCTTC 3 cut(s) 531, 1882, 4428
BspTNI GGTCTC 1 cut(s) 3547
BsrBI CCGCTC 1 cut(s) 3100
BsrDI GCAATG 3 cut(s) 577, 2112, 3764
BsrGI TGTACA 1 cut(s) 433
BssNAI GTATAC 1 cut(s) 3314
BssSI CACGAG 2 cut(s) 423, 606
BssT1I CCWWGG 4 cut(s) 165, 821, 871, 2245
Bst1107I GTATAC 1 cut(s) 3314
Bst2BI CACGAG 2 cut(s) 423, 606
Bst2UI CCWGG 5 cut(s) 678, 1884, 3000, 3636, 4011
Bst4CI ACNGT 9 cut(s) 730, 1055, 2526, 3342, 3593, 3773, 4020, 4049, 4472
Bst6I CTCTTC 8 cut(s) 276, 531, 960, 1029, 1644, 1882, 4307, 4428
BstAPI GCANNNNNTGC 3 cut(s) 2455, 2893, 3151
BstAUI TGTACA 1 cut(s) 433
BstC8I GCNNGC 5 cut(s) 973, 1370, 3959, 3963, 4195
BstDSI CCRYGG 2 cut(s) 165, 3592
BstENI CCTNNNNNAGG 3 cut(s) 870, 1080, 3607
BstFNI CGCG 3 cut(s) 450, 3016, 3869
BstH2I RGCGCY 2 cut(s) 208, 1614
BstHHI GCGC 3 cut(s) 207, 1539, 1613
BstMAI GTCTC 8 cut(s) 102, 361, 1082, 1646, 1743, 2935, 3547, 4234
BstNI CCWGG 5 cut(s) 678, 1884, 3000, 3636, 4011
BstNSI RCATGY 2 cut(s) 2182, 3268
BstPAI GACNNNNGTC 1 cut(s) 3364
BstSCI CCNGG 7 cut(s) 676, 1882, 2295, 2998, 3556, 3634, 4009
BstSFI CTRYAG 6 cut(s) 1373, 1529, 2663, 2876, 3024, 3507
BstSLI GKGCMC 1 cut(s) 713
BstUI CGCG 3 cut(s) 450, 3016, 3869
BstV2I GAAGAC 3 cut(s) 228, 3519, 4014
BstX2I RGATCY 6 cut(s) 1241, 1573, 2338, 3003, 3205, 4464
BstYI RGATCY 6 cut(s) 1241, 1573, 2338, 3003, 3205, 4464
BstZ17I GTATAC 1 cut(s) 3314
Bsu15I ATCGAT 1 cut(s) 4413
Bsu36I CCTNAGG 2 cut(s) 1042, 1422
BsuI GTATCC 2 cut(s) 828, 873
BsuTUI ATCGAT 1 cut(s) 4413
BtgI CCRYGG 2 cut(s) 165, 3592
BtgZI GCGATG 1 cut(s) 4140
BtrI CACGTC 2 cut(s) 626, 1737
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 8 cut(s) 110, 726, 1227, 1677, 1758, 3977, 4054, 4303
BtuMI TCGCGA 2 cut(s) 450, 3016
Cac8I GCNNGC 5 cut(s) 973, 1370, 3959, 3963, 4195
CaiI CAGNNNCTG 4 cut(s) 806, 1679, 2341, 3151
CfoI GCGC 3 cut(s) 207, 1539, 1613
Cfr13I GGNCC 9 cut(s) 476, 709, 710, 1469, 1834, 2094, 2139, 2810, 3399
ClaI ATCGAT 1 cut(s) 4413
CseI GACGC 4 cut(s) 329, 1192, 1899, 3875
Csp6I GTAC 4 cut(s) 434, 669, 2069, 2866
CspCI CAANNNNNGTGG 2 cut(s) 4357, 4392
CviQI GTAC 4 cut(s) 434, 669, 2069, 2866
DraI TTTAAA 2 cut(s) 1599, 4029
EaeI YGGCCR 7 cut(s) 3, 733, 1960, 2677, 2960, 3559, 4012
Eam1104I CTCTTC 8 cut(s) 276, 531, 960, 1029, 1644, 1882, 4307, 4428
EarI CTCTTC 8 cut(s) 276, 531, 960, 1029, 1644, 1882, 4307, 4428
Eco130I CCWWGG 4 cut(s) 165, 821, 871, 2245
Eco147I AGGCCT 1 cut(s) 4132
Eco24I GRGCYC 1 cut(s) 713
Eco31I GGTCTC 1 cut(s) 3547
Eco47I GGWCC 5 cut(s) 476, 1469, 1834, 2094, 2810
Eco57I CTGAAG 4 cut(s) 212, 783, 1302, 3823
Eco81I CCTNAGG 2 cut(s) 1042, 1422
Eco88I CYCGRG 1 cut(s) 3705
EcoNI CCTNNNNNAGG 3 cut(s) 870, 1080, 3607
EcoO109I RGGNCCY 3 cut(s) 476, 709, 2094
EcoRI GAATTC 3 cut(s) 1216, 3277, 4111
EcoRII CCWGG 5 cut(s) 676, 1882, 2998, 3634, 4009
EcoT14I CCWWGG 4 cut(s) 165, 821, 871, 2245
EcoT22I ATGCAT 2 cut(s) 202, 1938
EcoT38I GRGCYC 1 cut(s) 713
ErhI CCWWGG 4 cut(s) 165, 821, 871, 2245
Esp3I CGTCTC 2 cut(s) 1743, 4234
FaqI GGGAC 8 cut(s) 608, 1190, 1294, 1482, 1534, 2167, 2512, 3517
FauI CCCGC 3 cut(s) 3240, 4252, 4373
FauNDI CATATG 1 cut(s) 986
FbaI TGATCA 4 cut(s) 55, 2326, 2626, 2977
FblI GTMKAC 1 cut(s) 3313
FriOI GRGCYC 1 cut(s) 713
GlaI GCGC 3 cut(s) 206, 1538, 1612
GsuI CTGGAG 5 cut(s) 1536, 1914, 2345, 3260, 3390
HaeII RGCGCY 2 cut(s) 208, 1614
HapII CCGG 5 cut(s) 1158, 2206, 2296, 3558, 3741
HgaI GACGC 4 cut(s) 329, 1192, 1899, 3875
HhaI GCGC 3 cut(s) 207, 1539, 1613
Hin6I GCGC 3 cut(s) 205, 1537, 1611
HinP1I GCGC 3 cut(s) 205, 1537, 1611
HincII GTYRAC 4 cut(s) 2077, 3092, 3816, 4275
HindII GTYRAC 4 cut(s) 2077, 3092, 3816, 4275
HpaI GTTAAC 1 cut(s) 2077
HpaII CCGG 5 cut(s) 1158, 2206, 2296, 3558, 3741
HphI GGTGA 9 cut(s) 386, 1058, 1097, 1139, 2336, 3335, 3404, 3485, 3570
Hpy166II GTNNAC 9 cut(s) 302, 726, 1018, 2077, 3092, 3314, 3326, 3816, 4275
Hpy8I GTNNAC 9 cut(s) 302, 726, 1018, 2077, 3092, 3314, 3326, 3816, 4275
Hpy99I CGWCG 3 cut(s) 330, 345, 450
HpyCH4III ACNGT 9 cut(s) 730, 1055, 2526, 3342, 3593, 3773, 4020, 4049, 4472
HpyCH4IV ACGT 6 cut(s) 625, 1736, 2067, 3010, 3361, 4277
HpySE526I ACGT 6 cut(s) 625, 1736, 2067, 3010, 3361, 4277
HspAI GCGC 3 cut(s) 205, 1537, 1611
Kpn2I TCCGGA 2 cut(s) 1157, 3740
Ksp22I TGATCA 4 cut(s) 55, 2326, 2626, 2977
KspAI GTTAAC 1 cut(s) 2077
LguI GCTCTTC 3 cut(s) 531, 1882, 4428
MaeII ACGT 6 cut(s) 625, 1736, 2067, 3010, 3361, 4277
MbiI CCGCTC 1 cut(s) 3100
MfeI CAATTG 4 cut(s) 1689, 2411, 2738, 2897
MflI RGATCY 6 cut(s) 1241, 1573, 2338, 3003, 3205, 4464
MhlI GDGCHC 2 cut(s) 713, 3706
MlsI TGGCCA 4 cut(s) 1962, 2679, 2962, 4014
MluNI TGGCCA 4 cut(s) 1962, 2679, 2962, 4014
MlyI GAGTC 8 cut(s) 590, 1046, 1088, 1808, 2566, 3219, 3968, 4352
MmeI TCCRAC 7 cut(s) 1299, 2256, 2640, 3031, 3324, 3507, 4042
Mox20I TGGCCA 4 cut(s) 1962, 2679, 2962, 4014
Mph1103I ATGCAT 2 cut(s) 202, 1938
MroI TCCGGA 2 cut(s) 1157, 3740
MscI TGGCCA 4 cut(s) 1962, 2679, 2962, 4014
MslI CAYNNNNRTG 2 cut(s) 469, 2322
Msp20I TGGCCA 4 cut(s) 1962, 2679, 2962, 4014
MspA1I CMGCKG 4 cut(s) 2461, 2723, 2890, 4259
MspI CCGG 5 cut(s) 1158, 2206, 2296, 3558, 3741
MspR9I CCNGG 7 cut(s) 678, 1884, 2297, 3000, 3558, 3636, 4011
MunI CAATTG 4 cut(s) 1689, 2411, 2738, 2897
Mva1269I GAATGC 2 cut(s) 87, 2311
MvaI CCWGG 5 cut(s) 678, 1884, 3000, 3636, 4011
MvnI CGCG 3 cut(s) 450, 3016, 3869
NciI CCSGG 2 cut(s) 2297, 3558
NcoI CCATGG 1 cut(s) 165
NdeI CATATG 1 cut(s) 986
NheI GCTAGC 1 cut(s) 4193
NlaIV GGNNCC 9 cut(s) 477, 711, 1138, 1470, 2115, 3005, 3104, 3207, 3618
NmeAIII GCCGAG 2 cut(s) 761, 2515
NmuCI GTSAC 8 cut(s) 179, 608, 1127, 2595, 2685, 3392, 4049, 4438
NruI TCGCGA 2 cut(s) 450, 3016
NsiI ATGCAT 2 cut(s) 202, 1938
NspI RCATGY 2 cut(s) 2182, 3268
PaeR7I CTCGAG 1 cut(s) 3705
PasI CCCWGGG 1 cut(s) 3635
PceI AGGCCT 1 cut(s) 4132
PciI ACATGT 1 cut(s) 2178
PciSI GCTCTTC 3 cut(s) 531, 1882, 4428
PcsI WCGNNNNNNNCGW 3 cut(s) 274, 1742, 4283
PctI GAATGC 2 cut(s) 87, 2311
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 4 cut(s) 1478, 3215, 3489, 4103
PleI GAGTC 8 cut(s) 590, 1045, 1087, 1807, 2566, 3219, 3968, 4351
PpsI GAGTC 8 cut(s) 590, 1045, 1087, 1807, 2566, 3219, 3968, 4351
PpuMI RGGWCCY 2 cut(s) 476, 2094
PscI ACATGT 1 cut(s) 2178
PshAI GACNNNNGTC 1 cut(s) 3364
Psp1406I AACGTT 1 cut(s) 3010
Psp5II RGGWCCY 2 cut(s) 476, 2094
Psp6I CCWGG 5 cut(s) 676, 1882, 2998, 3634, 4009
PspGI CCWGG 5 cut(s) 676, 1882, 2998, 3634, 4009
PspN4I GGNNCC 9 cut(s) 477, 711, 1138, 1470, 2115, 3005, 3104, 3207, 3618
PspOMI GGGCCC 1 cut(s) 709
PspPI GGNCC 9 cut(s) 476, 709, 710, 1469, 1834, 2094, 2139, 2810, 3399
PspPPI RGGWCCY 2 cut(s) 476, 2094
PspXI VCTCGAGB 1 cut(s) 3705
PstI CTGCAG 3 cut(s) 1377, 2667, 2880
PstNI CAGNNNCTG 4 cut(s) 806, 1679, 2341, 3151
PsuI RGATCY 6 cut(s) 1241, 1573, 2338, 3003, 3205, 4464
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 2 cut(s) 2461, 2890
RruI TCGCGA 2 cut(s) 450, 3016
RsaI GTAC 4 cut(s) 435, 670, 2070, 2867
RsaNI GTAC 4 cut(s) 434, 669, 2069, 2866
RseI CAYNNNNRTG 2 cut(s) 469, 2322
SapI GCTCTTC 3 cut(s) 531, 1882, 4428
Sau96I GGNCC 9 cut(s) 476, 709, 710, 1469, 1834, 2094, 2139, 2810, 3399
SchI GAGTC 8 cut(s) 590, 1046, 1088, 1808, 2566, 3219, 3968, 4352
ScrFI CCNGG 7 cut(s) 678, 1884, 2297, 3000, 3558, 3636, 4011
SduI GDGCHC 2 cut(s) 713, 3706
SfcI CTRYAG 6 cut(s) 1373, 1529, 2663, 2876, 3024, 3507
Sfr274I CTCGAG 1 cut(s) 3705
SinI GGWCC 5 cut(s) 476, 1469, 1834, 2094, 2810
SlaI CTCGAG 1 cut(s) 3705
SmiMI CAYNNNNRTG 2 cut(s) 469, 2322
SmlI CTYRAG 6 cut(s) 358, 1452, 3095, 3231, 3705, 3831
SmoI CTYRAG 6 cut(s) 358, 1452, 3095, 3231, 3705, 3831
SseBI AGGCCT 1 cut(s) 4132
SspI AATATT 1 cut(s) 3427
StuI AGGCCT 1 cut(s) 4132
StyD4I CCNGG 7 cut(s) 676, 1882, 2295, 2998, 3556, 3634, 4009
StyI CCWWGG 4 cut(s) 165, 821, 871, 2245
TaaI ACNGT 9 cut(s) 730, 1055, 2526, 3342, 3593, 3773, 4020, 4049, 4472
TaiI ACGT 6 cut(s) 628, 1739, 2070, 3013, 3364, 4280
TaqII GACCGA 2 cut(s) 1851, 2844
TatI WGTACW 1 cut(s) 433
TauI GCSGC 6 cut(s) 1071, 1412, 2723, 3657, 3901, 4340
TscAI CASTG 8 cut(s) 110, 733, 1227, 1684, 1758, 3984, 4054, 4303
TseFI GTSAC 8 cut(s) 179, 608, 1127, 2595, 2685, 3392, 4049, 4438
Tsp45I GTSAC 8 cut(s) 179, 608, 1127, 2595, 2685, 3392, 4049, 4438
TspGWI ACGGA 6 cut(s) 81, 566, 2019, 2673, 2844, 2922
TspRI CASTG 8 cut(s) 110, 733, 1227, 1684, 1758, 3984, 4054, 4303
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 4 cut(s) 1478, 3215, 3489, 4103
VpaK11BI GGWCC 5 cut(s) 476, 1469, 1834, 2094, 2810
XagI CCTNNNNNAGG 3 cut(s) 870, 1080, 3607
XapI RAATTY 8 cut(s) 715, 1216, 1669, 1805, 2692, 2728, 3277, 4111
XceI RCATGY 2 cut(s) 2182, 3268
XcmI CCANNNNNNNNNTGG 2 cut(s) 916, 3269
XhoI CTCGAG 1 cut(s) 3705
XmaJI CCTAGG 1 cut(s) 871
XmiI GTMKAC 1 cut(s) 3313
Zsp2I ATGCAT 2 cut(s) 202, 1938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.