Rh4BG299200

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005720
Physical Location & Seq
Forward (+)
47068437 .. 47075838
7402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG299200.1

Sequence Viewer

Length: 4557 bp
ATGGCGGAACGCAAGCTCGATCTCCCCGACGATCTCCTCTCCTCCAAACCCTCCGATCAGTCCTGGACCTCCAAAGTTGAAGTTTCTGGAGGAAATGATGAGGAAAAGGTGCTTATGGGGTCATCTGATGAGTTGAAAGATCCAGCCGCCTTGGATAGCAGCATACCTTTGTCACCCCAGTGGCTTTATGCCAAGCCGATTGAGTCTAAGTTGGAAATGCGTGGTCCAACTTCACTCGGAAACTCCTCTGACAGCAACCAGAAGGACGGTTGGCGGTTGGAAGGATCTGAGGACAAGAAAGATTGGAGGCGACCTGCCACTGAAAGTGAAAGTAGCCGTCGTTGGCGTGAAGAGGAAAGAGAAACTAGCTTGCTTGGTGGTAGAAGAGATCGTAGGAAAACAGAACGCCGTGCTGACAATATTCCATTGAGGGACACAACTGACAGTAGAGCATTGCCTACAACTGACAGGTGGCATGATGGCCGCAATGAAGTACGGCGTGATAGCAAATGGTCGTCTAGGTGGGGTCCTGAAGACAAAGAAAAGGAGCCCCGAACTGAGAAGAGAACAGATGTGGAGAAGGATGATGCTCACAGTAACAGTGAGAGTCAATCTCTTGGAGCTAACAACCGTTCAGCTGCTGAGCGTGAGTCAGATTCTCGGGATAAATGGAGGCCACGACATAGAATGGAGGTTCAAACTGGGGGGTCAGCTACTTACCGTGCTGCACCTGGATTTGGACTAGAAAGAGGACGGGTGGAGGGTTCGAATTTGGGATTTACCCTGGGGCGAGGAAGGTCAAGTGGTATTGGGAGATCCGCAGGCACTTTTGGCTCTGCTCTTTCAGGAAAGAGTGAAGGTGTCCCTGGGAAACCAAGGCACTCAGCTGATGGTTTTTGCTACCCGAGGGGAAAGCTTCTTGATATTTATCGGCAGCGAAAGCCTGAACTATCTTTTGATACCATGCCAGATGAGATGGAGGAGGCCCCCTCACTAACTCAAGTTGATTTTGTTGAACCATTGGCTTTTCATGCCCCTGATGCTGATGAGGAGGCCATCATTAGCGATATATGGAAGGGAAAAATTACAAGTAGTGGGGTAGTATACAATTCATTCAGAAAGGGGAGATCAACTGAAAATATTACAGGTGTGGGAGACGTAGAAGCTGCTGATGGAGTTCTGGGCAATTTGCCCTCGACTGTCACTCAAGAGACTAGCACCTTTCAGGAGGCTGCAAATGCTGATGACTATGGGACTTTGTGGAATTATGGTTTGCAGAGGAATGTGATGAATGGAAAAGATGTTGGTCATAAAGAAAGTGATAACAGAGCTACTGAAGGAAAGGACCTTGATGGAATTTCCCTATCAATTCAAAAAAGTAATGGTATCTATGGTGATGTTGAGACTGATGGTTCTTATGATAGTGCAAATCAGCTTCATGGTGGTGGCAGTAGGAAAATTGGAGATTCTGCCTTTTCAAATCATCCTGTATCTGATGACATTGAGTTTGCCAATTATTCCGAAATAAGATCCAACCTTCCCGACGTTTCAAATACTCTCTATGGTTTGGCCTCTTCTGAGCAGAATGAGAATAGCAACCCTAGAGTGAAAGAGTTAGAAATGGGTGTTCATCCTGAGGGTTTGTATTACTACTACCTTGATCCTCAAGGGGTGACCCAGGGGCCATATCAGGGGTTAGACATCATTTCATGGTTTGAACAAGGTTTCTTTGGGATAGACTTGCTTGTTCGCTTGGAGGATGCTCCTGAAGGAACACCTTTCAAAGAGTTGGGGGAGTTCATGCCATATCTTAATTCTTGGGGTGAGAATGGCAGTATCATAAATCCAAGTTCTAAGCTAGAAGAATCTGGTGGCTTAGGAGGGAGTCTGGAGTCCAGTTTACCCTTTTCTGCAGCAGTTTCAGACAGTAACTATGCATTTCTGGGAAATGACCATCAGCGGCCCATGTCTGAACTAAACAGCCTTTCAGCTCAGCATATTCAGCCAAGAATTTCCGAGCCTGAAGCTCCACTACAACTACATTCAAGGGGTCAAAGCTTTAATGATTTTGTTGAACAAGATGAAGATATTGTGTTTCCTGGAATACATGGAACTGCTGCCTATTCTACTGCACGATCTTCTGGGAGCATTCACGATTCTATGGCAAACTCTATTAATCACCTTCCTCCCCCAACTGAATTGACAGAATCTGGTGTGCCTATTCAGAATGATAATAAGTTGCATCCCTTTGGCTTGCTATGGTCTGAGCTAGAAAGTGGCCAATCAAAGCATTCAAAGTCGGCCAATATGCCATCTAGTAAGGGGAGGGCTGTTCCATTTGGTGCAAATCCTGATCCAGCAATTGCTGAGACGTGGTCTGATCTTCATAGAAAAAGTTCAGTCTCTGATCCGAACTTGTATCAGGAAATGTTGACTCCTCGCCAATTGTCGCACATGGAACAGGAACCCAGTCATTATGATTTAGCAGAGCAGCTTATGTCGCAGCAAATTCGACAGCAGCAGCAACTCCAACAGCGAAATATGTTGTCTGGTTTTGCACACTTGAATGACAATGTATTAGATCCTTTGCAAAATCAGAATCTTATTCACCATCAACAGTTAGCCAACCATTCTGCAGCAGATCTGGATCACCTTTTGGCACTTCAGAGGCAGGCTCAGCTCGAGCAACATCAACTGCAACAACAGCAGCAGTTTCATCAACAGCAGAAGCTTTTACAGGAGCAACAGCAATCTCAAGTACAACAGGTGCTTTTTGAACAATTGTTGCGTGGTCAAATGCATGATCCTACACTTAGGCAGCCACATGTTGATCCTGTAAGAGCAAACAATGTTATTGATCAGGTTCTATTAGAGCAGCAGCTTCTACGTGAACTTCAGCAACGGTCTCACCATCTTCCAAGGCATGTTGATCCAACTATGGAGCAGTTAATTCAAGCAAAGTTTGGTCATACACCTCAAGGACATCAAACAGATTTATTTGAGCTACTGTCTCGTGCACAGCATGAACAGATTCAGTCGCTGGAACATCAGATGCATGCCAGGCAGTTACCCATGGGAATAAGGCAGCGAATGGAAGAAGAGAGGCATATCGGTTCTGTCTGGCCAGCTGAGGAATCAAATCAGATTTTCAGGACCCATGCTGGTAATCATGGTCACCGAGGGCACTCTTCTGGCTTTAATCCTTTAGATTTTTATCAGAGGCAGCAGAGGCCATCTCATGAGGAGCAGCTGAATCACCTTGACCGGAATCTTTCATTACAGGATCGACTTCAGCAAGGTTTTTATGAGCCTGGCTCGCTGCCGTTTGAGCGGTCAATGTCATTACCTGCTGGTGCACCAGGGATGAACTTGGATGTAGTAAATGCTATGGCTCGTGCCCAGGGTTTAGATATGCAGGATTCCATTGGACGTATGCAGTCGGCTGGCCAGTCGGGTCAATTTTCGTCTGGAATCCCTTCCCACAATGGTCATCATCCTCAAGTTCCTAACCAATTTCATGTTTCACATTTGGATGCAATTGAGGGACACTGGCCTGAGAAGAATGATCAGCTTGAGAATGACTGGATGGACGCTCGGTTTCAGCAGCTGCATATTAATGCTGAGAGGCAGAAAAGGGAATCAGAGATCAAGAATACTTCTCAGGATCAAAATTTATGGATGTCAGATGGAATTAATGATGAGAACTCAAAGAGACTACTAATGGATTTACTCCATCAAAAATCTGGCCATCAACCTAGCGAGTCGTTGAATGCGATGAGCAATGGGATGTTCTCTGATAAAAGACTGCCATCTGGCCACTACTCTGGATCAAGCTCCACAAACCACCTGTTCAATCTCCATGCAGACCAAGAAGCAGGTCTAAATAACTCATTTCGTGTAGGGTCATTTGGTTCTAATCCAGGTGAACTCCTACAGGAAGAGCAGGCCAGCAGTGTGGAAAGCAATGAAAAGTTGATGTATAGATCTAATTCTGGAGCATTGGCTGATAGAGAGTCATTTCTGGCAGGAATGAATGCAACGAGCCAGTCGCTTTACACAAATTCTAACATGATAAGTAAGTCTTCTATAGGTAAAGAACTTTCTGAGTTGGAGGGCAGGAAGCGGGGGTCCAAAAGTGAGGGGATAATTATGGGTCGATCTTTTGAGACTCAAGAACGAATGGTTGAACAAGCAGGGTTATCTGCAACAAATTTTGAGGAGAGGTCAAAAAACGCCCATAGCATGAATTCTTCCTCCGGTGTTTCAGGTGGGAATGCTGGTTTTTATGGTGACAAGACTGGCCGAAGTAATTCATTTGTCGAACAAACTGCCAAGGATCGGGCTCCCATCCCATCCAAAGGGCAAGAAAATATTCTGCTGAGACGACCTTCTGTCCCAAGTGCTTCAGCATCGCAAGAAGGATTATCAGAACTGATATCAGATCCAGTTCTTAGAGGAAAAAATTCAAGTGCTGCTTCAGATGGTATTTTGTTTCTAACTCATACTAGTTTCTTATCTTGTATGGTTACATTTTGTTGGAGGGGGAGTGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1518

Amino Acids

169.02

Weight (kDa)

5.42

Isoelectric Point (pI)

57.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 550 - 595 6.4e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 322, 3355, 3866
AccBSI CCGCTC 1 cut(s) 3331
AccI GTMKAC 1 cut(s) 1104
AciI CCGC 8 cut(s) 5, 147, 274, 484, 819, 1960, 3331, 4123
AcoI YGGCCR 8 cut(s) 481, 2278, 2301, 3122, 3445, 3745, 3814, 4300
AcsI RAATTY 9 cut(s) 769, 1356, 2010, 2508, 3670, 4060, 4210, 4246, 4462
AcuI CTGAAG 9 cut(s) 552, 1356, 1788, 2043, 2648, 2879, 3275, 4389, 4461
AfaI GTAC 2 cut(s) 495, 2760
AfiI CCNNNNNNNGG 4 cut(s) 737, 1691, 4338, 4358
AflIII ACRYGT 1 cut(s) 2824
AhdI GACNNNNNGTC 1 cut(s) 4391
AhlI ACTAGT 1 cut(s) 4505
AjiI CACGTC 1 cut(s) 2373
AleI CACNNNNGTG 1 cut(s) 178
Alw21I GWGCWC 2 cut(s) 3019, 3358
Alw44I GTGCAC 2 cut(s) 3015, 3354
AlwNI CAGNNNCTG 5 cut(s) 641, 2210, 2405, 3040, 3607
Ama87I CYCGRG 3 cut(s) 660, 904, 2681
ApaLI GTGCAC 2 cut(s) 3015, 3354
ApoI RAATTY 9 cut(s) 769, 1356, 2010, 2508, 3670, 4060, 4210, 4246, 4462
ArsI GACNNNNNNTTYG 2 cut(s) 1490, 1522
AseI ATTAAT 3 cut(s) 2175, 3615, 3693
Asp700I GAANNNNTTC 6 cut(s) 2395, 3030, 3475, 4309, 4371, 4462
AspS9I GGNCC 9 cut(s) 66, 224, 527, 985, 1345, 1682, 1962, 3153, 4128
AsuII TTCGAA 1 cut(s) 767
AvaI CYCGRG 3 cut(s) 660, 904, 2681
AvaII GGWCC 6 cut(s) 66, 224, 527, 1345, 3153, 4128
AxyI CCTNAGG 1 cut(s) 1635
BaeGI GKGCMC 4 cut(s) 3019, 3186, 3358, 3400
BalI TGGCCA 5 cut(s) 2280, 3124, 3447, 3747, 3816
BanII GRGCYC 2 cut(s) 552, 4345
BarI GAAGNNNNNNTAC 2 cut(s) 2016, 2048
BauI CACGAG 2 cut(s) 3012, 3393
BbsI GAAGAC 2 cut(s) 540, 4074
Bbv12I GWGCWC 2 cut(s) 3019, 3358
BbvCI CCTCAGC 1 cut(s) 3129
BceAI ACGGC 4 cut(s) 321, 393, 512, 3307
BcgI CGANNNNNNTGC 4 cut(s) 2492, 2526, 4310, 4344
BclI TGATCA 2 cut(s) 2857, 3565
BcuI ACTAGT 1 cut(s) 4505
BfmI CTRYAG 4 cut(s) 1911, 2634, 3932, 4086
BfuAI ACCTGC 3 cut(s) 322, 3355, 3866
BglII AGATCT 2 cut(s) 2641, 3983
BlpI GCTNAGC 3 cut(s) 642, 1992, 2676
Bme18I GGWCC 6 cut(s) 66, 224, 527, 1345, 3153, 4128
BmeRI GACNNNNNGTC 1 cut(s) 4391
BmeT110I CYCGRG 3 cut(s) 660, 904, 2681
BmgBI CACGTC 1 cut(s) 2373
BmgT120I GGNCC 9 cut(s) 66, 224, 527, 985, 1345, 1682, 1962, 3153, 4128
BmiI GGNNCC 8 cut(s) 528, 549, 987, 1683, 2466, 3155, 4129, 4344
BmrI ACTGGG 3 cut(s) 172, 711, 2463
BmsI GCATC 7 cut(s) 577, 1030, 1750, 2251, 3042, 3523, 4418
BmuI ACTGGG 3 cut(s) 172, 711, 2463
BpiI GAAGAC 2 cut(s) 540, 4074
BpmI CTGGAG 3 cut(s) 108, 1910, 4014
Bpu10I CCTNAGC 2 cut(s) 1876, 3129
Bpu1102I GCTNAGC 3 cut(s) 642, 1992, 2676
Bpu14I TTCGAA 1 cut(s) 767
BpuEI CTTGAG 8 cut(s) 984, 1191, 1650, 2739, 2961, 3483, 3593, 4155
BsaAI YACGTR 1 cut(s) 2888
BsaBI GATNNNNATC 3 cut(s) 927, 2646, 3213
BsaI GGTCTC 1 cut(s) 2910
BsaWI WCCGGW 2 cut(s) 3264, 4256
BsaXI ACNNNNNCTCC 1 cut(s) 4531
Bsc4I CCNNNNNNNGG 4 cut(s) 737, 1691, 4338, 4358
Bse21I CCTNAGG 1 cut(s) 1635
Bse3DI GCAATG 4 cut(s) 452, 493, 3787, 3970
Bse8I GATNNNNATC 3 cut(s) 927, 2646, 3213
BseJI GATNNNNATC 3 cut(s) 927, 2646, 3213
BseLI CCNNNNNNNGG 4 cut(s) 737, 1691, 4338, 4358
BseMI GCAATG 4 cut(s) 452, 493, 3787, 3970
BseRI GAGGAG 8 cut(s) 26, 31, 235, 995, 1064, 2427, 3257, 4232
BseSI GKGCMC 4 cut(s) 3019, 3186, 3358, 3400
BsgI GTGCAG 2 cut(s) 711, 2115
BsiHKAI GWGCWC 2 cut(s) 3019, 3358
BsiHKCI CYCGRG 3 cut(s) 660, 904, 2681
BsiSI CCGG 2 cut(s) 3265, 4257
BslFI GGGAC 5 cut(s) 446, 848, 1267, 3558, 4379
BslI CCNNNNNNNGG 4 cut(s) 737, 1691, 4338, 4358
BsmBI CGTCTC 3 cut(s) 1149, 2363, 4375
BsmFI GGGAC 5 cut(s) 446, 848, 1267, 3558, 4379
BsmI GAATGC 5 cut(s) 2148, 2290, 3775, 4039, 4279
Bso31I GGTCTC 1 cut(s) 2910
BsoBI CYCGRG 3 cut(s) 660, 904, 2681
Bsp119I TTCGAA 1 cut(s) 767
Bsp1286I GDGCHC 6 cut(s) 552, 3019, 3186, 3358, 3400, 4345
Bsp1720I GCTNAGC 3 cut(s) 642, 1992, 2676
Bsp19I CCATGG 1 cut(s) 3072
BspACI CCGC 8 cut(s) 5, 147, 274, 484, 819, 1960, 3331, 4123
BspHI TCATGA 1 cut(s) 3238
BspLI GGNNCC 8 cut(s) 528, 549, 987, 1683, 2466, 3155, 4129, 4344
BspMAI CTGCAG 2 cut(s) 1915, 2638
BspMI ACCTGC 3 cut(s) 322, 3355, 3866
BspQI GCTCTTC 1 cut(s) 3933
BspT104I TTCGAA 1 cut(s) 767
BspTNI GGTCTC 1 cut(s) 2910
BsrBI CCGCTC 1 cut(s) 3331
BsrDI GCAATG 4 cut(s) 452, 493, 3787, 3970
BssNAI GTATAC 1 cut(s) 1105
BssSI CACGAG 2 cut(s) 3012, 3393
BssT1I CCWWGG 5 cut(s) 150, 875, 2918, 3072, 4332
Bst1107I GTATAC 1 cut(s) 1105
Bst2BI CACGAG 2 cut(s) 3012, 3393
Bst6I CTCTTC 7 cut(s) 345, 379, 557, 1579, 3093, 3193, 3933
BstBAI YACGTR 1 cut(s) 2888
BstBI TTCGAA 1 cut(s) 767
BstDSI CCRYGG 1 cut(s) 3072
BstEII GGTNACC 2 cut(s) 1672, 3173
BstNSI RCATGY 3 cut(s) 2828, 2927, 3059
BstPI GGTNACC 2 cut(s) 1672, 3173
BstSFI CTRYAG 4 cut(s) 1911, 2634, 3932, 4086
BstSLI GKGCMC 4 cut(s) 3019, 3186, 3358, 3400
BstV2I GAAGAC 2 cut(s) 540, 4074
BstX2I RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3983, 4441
BstXI CCANNNNNNTGG 2 cut(s) 3824, 3955
BstYI RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3983, 4441
BstZ17I GTATAC 1 cut(s) 1105
Bsu36I CCTNAGG 1 cut(s) 1635
BtgI CCRYGG 1 cut(s) 3072
BtgZI GCGATG 2 cut(s) 3788, 4395
BtrI CACGTC 1 cut(s) 2373
BtsI GCAGTG 1 cut(s) 3958
BtsIMutI CAGTG 5 cut(s) 185, 318, 607, 3547, 3958
BveI ACCTGC 3 cut(s) 322, 3355, 3866
CaiI CAGNNNCTG 5 cut(s) 641, 2210, 2405, 3040, 3607
CciI TCATGA 1 cut(s) 3238
Cfr13I GGNCC 9 cut(s) 66, 224, 527, 985, 1345, 1682, 1962, 3153, 4128
CseI GACGC 1 cut(s) 3599
Csp6I GTAC 2 cut(s) 494, 2759
CviQI GTAC 2 cut(s) 494, 2759
DriI GACNNNNNGTC 1 cut(s) 4391
EaeI YGGCCR 8 cut(s) 481, 2278, 2301, 3122, 3445, 3745, 3814, 4300
Eam1104I CTCTTC 7 cut(s) 345, 379, 557, 1579, 3093, 3193, 3933
Eam1105I GACNNNNNGTC 1 cut(s) 4391
EarI CTCTTC 7 cut(s) 345, 379, 557, 1579, 3093, 3193, 3933
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 5 cut(s) 150, 875, 2918, 3072, 4332
Eco24I GRGCYC 2 cut(s) 552, 4345
Eco31I GGTCTC 1 cut(s) 2910
Eco32I GATATC 1 cut(s) 4437
Eco47I GGWCC 6 cut(s) 66, 224, 527, 1345, 3153, 4128
Eco57I CTGAAG 9 cut(s) 552, 1356, 1788, 2043, 2648, 2879, 3275, 4389, 4461
Eco81I CCTNAGG 1 cut(s) 1635
Eco88I CYCGRG 3 cut(s) 660, 904, 2681
Eco91I GGTNACC 2 cut(s) 1672, 3173
EcoO109I RGGNCCY 4 cut(s) 527, 985, 1345, 3153
EcoO65I GGTNACC 2 cut(s) 1672, 3173
EcoRI GAATTC 1 cut(s) 4246
EcoRV GATATC 1 cut(s) 4437
EcoT14I CCWWGG 5 cut(s) 150, 875, 2918, 3072, 4332
EcoT22I ATGCAT 3 cut(s) 1939, 2802, 3057
EcoT38I GRGCYC 2 cut(s) 552, 4345
ErhI CCWWGG 5 cut(s) 150, 875, 2918, 3072, 4332
Esp3I CGTCTC 3 cut(s) 1149, 2363, 4375
FalI AAGNNNNNCTT 4 cut(s) 4066, 4098, 4459, 4491
FaqI GGGAC 5 cut(s) 446, 848, 1267, 3558, 4379
FauI CCCGC 1 cut(s) 4116
FbaI TGATCA 2 cut(s) 2857, 3565
FblI GTMKAC 1 cut(s) 1104
FriOI GRGCYC 2 cut(s) 552, 4345
GsuI CTGGAG 3 cut(s) 108, 1910, 4014
HapII CCGG 2 cut(s) 3265, 4257
HgaI GACGC 1 cut(s) 3599
HincII GTYRAC 1 cut(s) 2433
HindII GTYRAC 1 cut(s) 2433
HindIII AAGCTT 3 cut(s) 914, 2056, 2729
HpaII CCGG 2 cut(s) 3265, 4257
Hpy166II GTNNAC 7 cut(s) 1105, 1901, 2433, 2891, 3017, 3356, 3926
Hpy8I GTNNAC 7 cut(s) 1105, 1901, 2433, 2891, 3017, 3356, 3926
Hpy99I CGWCG 3 cut(s) 32, 342, 1547
HpyCH4IV ACGT 5 cut(s) 1158, 1545, 2372, 2887, 3430
HpySE526I ACGT 5 cut(s) 1158, 1545, 2372, 2887, 3430
Ksp22I TGATCA 2 cut(s) 2857, 3565
LguI GCTCTTC 1 cut(s) 3933
LweI GCATC 7 cut(s) 577, 1030, 1750, 2251, 3042, 3523, 4418
MaeII ACGT 5 cut(s) 1158, 1545, 2372, 2887, 3430
MbiI CCGCTC 1 cut(s) 3331
MfeI CAATTG 4 cut(s) 2361, 2444, 2780, 3537
MflI RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3983, 4441
MhlI GDGCHC 6 cut(s) 552, 3019, 3186, 3358, 3400, 4345
MlsI TGGCCA 5 cut(s) 2280, 3124, 3447, 3747, 3816
MluNI TGGCCA 5 cut(s) 2280, 3124, 3447, 3747, 3816
MlyI GAGTC 9 cut(s) 212, 616, 659, 1894, 1901, 2428, 3770, 4022, 4162
MmeI TCCRAC 8 cut(s) 192, 251, 258, 1557, 2554, 2957, 4089, 4517
Mox20I TGGCCA 5 cut(s) 2280, 3124, 3447, 3747, 3816
Mph1103I ATGCAT 3 cut(s) 1939, 2802, 3057
MroXI GAANNNNTTC 6 cut(s) 2395, 3030, 3475, 4309, 4371, 4462
MscI TGGCCA 5 cut(s) 2280, 3124, 3447, 3747, 3816
MseI TTAA 7 cut(s) 1812, 2061, 2175, 2948, 3198, 3615, 3693
MslI CAYNNNNRTG 5 cut(s) 178, 1443, 2565, 3531, 3615
Msp20I TGGCCA 5 cut(s) 2280, 3124, 3447, 3747, 3816
MspA1I CMGCKG 6 cut(s) 638, 887, 1960, 3128, 3250, 3607
MspI CCGG 2 cut(s) 3265, 4257
MunI CAATTG 4 cut(s) 2361, 2444, 2780, 3537
Mva1269I GAATGC 5 cut(s) 2148, 2290, 3775, 4039, 4279
NcoI CCATGG 1 cut(s) 3072
NlaIV GGNNCC 8 cut(s) 528, 549, 987, 1683, 2466, 3155, 4129, 4344
NmuCI GTSAC 6 cut(s) 171, 1201, 1672, 3173, 4289, 4550
NsiI ATGCAT 3 cut(s) 1939, 2802, 3057
NspI RCATGY 3 cut(s) 2828, 2927, 3059
NspV TTCGAA 1 cut(s) 767
OliI CACNNNNGTG 1 cut(s) 178
PaeI GCATGC 1 cut(s) 3059
PaeR7I CTCGAG 1 cut(s) 2681
PagI TCATGA 1 cut(s) 3238
PasI CCCWGGG 4 cut(s) 784, 866, 1678, 3400
PciI ACATGT 1 cut(s) 2824
PciSI GCTCTTC 1 cut(s) 3933
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 3770
PctI GAATGC 5 cut(s) 2148, 2290, 3775, 4039, 4279
PdmI GAANNNNTTC 6 cut(s) 2395, 3030, 3475, 4309, 4371, 4462
PflFI GACNNNGTC 1 cut(s) 2374
PfoI TCCNGGA 2 cut(s) 62, 2098
PleI GAGTC 9 cut(s) 211, 615, 658, 1893, 1900, 2428, 3769, 4021, 4162
PpsI GAGTC 9 cut(s) 211, 615, 658, 1893, 1900, 2428, 3769, 4021, 4162
Ppu21I YACGTR 1 cut(s) 2888
PpuMI RGGWCCY 3 cut(s) 527, 1345, 3153
PscI ACATGT 1 cut(s) 2824
PshBI ATTAAT 3 cut(s) 2175, 3615, 3693
Psp5II RGGWCCY 3 cut(s) 527, 1345, 3153
PspEI GGTNACC 2 cut(s) 1672, 3173
PspN4I GGNNCC 8 cut(s) 528, 549, 987, 1683, 2466, 3155, 4129, 4344
PspPI GGNCC 9 cut(s) 66, 224, 527, 985, 1345, 1682, 1962, 3153, 4128
PspPPI RGGWCCY 3 cut(s) 527, 1345, 3153
PspXI VCTCGAGB 1 cut(s) 2681
PstI CTGCAG 2 cut(s) 1915, 2638
PstNI CAGNNNCTG 5 cut(s) 641, 2210, 2405, 3040, 3607
PsuI RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3983, 4441
PsyI GACNNNGTC 1 cut(s) 2374
PvuII CAGCTG 5 cut(s) 638, 887, 3128, 3250, 3607
RsaI GTAC 2 cut(s) 495, 2760
RsaNI GTAC 2 cut(s) 494, 2759
RseI CAYNNNNRTG 5 cut(s) 178, 1443, 2565, 3531, 3615
SapI GCTCTTC 1 cut(s) 3933
SaqAI TTAA 7 cut(s) 1812, 2061, 2175, 2948, 3198, 3615, 3693
Sau96I GGNCC 9 cut(s) 66, 224, 527, 985, 1345, 1682, 1962, 3153, 4128
SchI GAGTC 9 cut(s) 212, 616, 659, 1894, 1901, 2428, 3770, 4022, 4162
SduI GDGCHC 6 cut(s) 552, 3019, 3186, 3358, 3400, 4345
SfaNI GCATC 7 cut(s) 577, 1030, 1750, 2251, 3042, 3523, 4418
SfcI CTRYAG 4 cut(s) 1911, 2634, 3932, 4086
Sfr274I CTCGAG 1 cut(s) 2681
SfuI TTCGAA 1 cut(s) 767
SinI GGWCC 6 cut(s) 66, 224, 527, 1345, 3153, 4128
SlaI CTCGAG 1 cut(s) 2681
SmiMI CAYNNNNRTG 5 cut(s) 178, 1443, 2565, 3531, 3615
SmlI CTYRAG 9 cut(s) 999, 1206, 1665, 2681, 2754, 2976, 3498, 3572, 4170
SmoI CTYRAG 9 cut(s) 999, 1206, 1665, 2681, 2754, 2976, 3498, 3572, 4170
SpeI ACTAGT 1 cut(s) 4505
SphI GCATGC 1 cut(s) 3059
SsiI CCGC 8 cut(s) 5, 147, 274, 484, 819, 1960, 3331, 4123
SspI AATATT 3 cut(s) 421, 1141, 4372
StyI CCWWGG 5 cut(s) 150, 875, 2918, 3072, 4332
TaiI ACGT 5 cut(s) 1161, 1548, 2375, 2890, 3433
TaqI TCGA 8 cut(s) 18, 767, 1196, 2512, 2682, 3286, 4156, 4320
TatI WGTACW 1 cut(s) 2758
TauI GCSGC 3 cut(s) 149, 486, 1963
Tru1I TTAA 7 cut(s) 1812, 2061, 2175, 2948, 3198, 3615, 3693
Tru9I TTAA 7 cut(s) 1812, 2061, 2175, 2948, 3198, 3615, 3693
TscAI CASTG 5 cut(s) 185, 325, 607, 3554, 3958
TseFI GTSAC 6 cut(s) 171, 1201, 1672, 3173, 4289, 4550
Tsp45I GTSAC 6 cut(s) 171, 1201, 1672, 3173, 4289, 4550
TspRI CASTG 5 cut(s) 185, 325, 607, 3554, 3958
Tth111I GACNNNGTC 1 cut(s) 2374
VneI GTGCAC 2 cut(s) 3015, 3354
VpaK11BI GGWCC 6 cut(s) 66, 224, 527, 1345, 3153, 4128
VspI ATTAAT 3 cut(s) 2175, 3615, 3693
XapI RAATTY 9 cut(s) 769, 1356, 2010, 2508, 3670, 4060, 4210, 4246, 4462
XceI RCATGY 3 cut(s) 2828, 2927, 3059
XcmI CCANNNNNNNNNTGG 1 cut(s) 3740
XhoI CTCGAG 1 cut(s) 2681
XmiI GTMKAC 1 cut(s) 1104
XmnI GAANNNNTTC 6 cut(s) 2395, 3030, 3475, 4309, 4371, 4462
Zsp2I ATGCAT 3 cut(s) 1939, 2802, 3057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.