Rw4G025390

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005720
Physical Location & Seq
Forward (+)
51467336 .. 51474076
6741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025390.1

Sequence Viewer

Length: 4527 bp
ATGGCGGAACGCAAGCTCGATCTCCCCGACGATCTCCTCTCCTCCAAACCCTCCGATCAGTCCTGGACCTCCAAAGTTGAAGTTTCTGGAGGAAATGATGAGGAAAAGGTGCTTATGGGGTCATCTGATGAGTTGAAAGATCCAGCAGCCTTGGATAGCAGCATACCTTTGTCACCGCAGTGGCTTTATGCCAAGCCGATTGAGTCTAAGTTGGAAATGCGTGGTCCAACTTCACTCGGAAACTCCACTGACAGCAACCAGAAGGACGGTTGGCGGTTGGAAGGATCTGAGGACAAGAAAGATTGGAGGCGACCTGCTACTGAAAGTGAAAGTAGCCGTCGTTGGCGAGAAGAGGAAAGAGAAACTAGCTTGCTTGGTGGTAGAAGAGATCGTAGGAAAACAGAACGCCTACGGCGTGATAGCAAATGGTCGTCTAGGTGGGGACCTGAAGACAAAGAAAAGGAGCCCCGAACTGAGAAGAGAACAGATGTGGAGAAGGATGATGCTCACAGTAACAGTGACAGTCAATCTCTTGGAGCTAACAACCGTTCAGCTGCTGAGTCAGATTCTCGGGATAAATGGAGGCCACGACATAGAATGGAGGTTCAAACTGGGGGGTCAGCTACTTATCCGTGCTGCACCTGGATTTGGACTAGAAAGAGGACGGGTGGAGGGTTCGAATTTGGGATTTACCCTGGGGCGAGGAAGGTCAAGTGGTGTCCTGGGAAACCAAGGCACTCAGCTGATGGTTTTTGCTACCCGAGGGGAAAGCTTCTTGATATTTATCGGCAGCGAAAGCCTGAACTCTCTTTTGATACCATGCCAGATGAGATGGAGGAGGCCCCCTCACTAACTCAAGTTGATTTTGTTGAACCATTGGCTTTTCATGCCCCTGATGCTGATGAGGAGGCCATCCTTAGCGATATATGGAAGGGAAAAATTACAAGTAGTGGGGTAGTATACAATTCATTCAGAAAGGGGAGATCAACTGAAAATATTACAGGTGTGGGAGACTTAGAAGCTGCTGATGGAGTGCTGGGCAATTTGCCCTCGACTGTCACTCAAGAGACTAGCACCTTTCAGGAGGCTGCAACTGCTGATGACTATGGGACTTTGTGGAATTATGGTTCACAGAGGAATGTGATAAATGGAAAAGATGTTGGTCATAAAGAAAGTGATAACAGAGCTACTGAAGGAAAGGACCTTGATGGAATTTCCCTATCAACTCAAAAAAGTAATGGTATCTATGGTGATGTTGAGACTGATGGTTCTTATGATAGTGCAAATCAGCTTCATGTTGGTGGCAATGGGAAAATTGGAGATTCTGCCTTTTCAAATCATCCTGTATCTGATGACATTGTGTTTGCCAATTATTCCGAAATAAGATCCAACCTTCCCGACGTTTCAAATACTCTCTATGGTTTGGCCTCTTCTGAGCAGAATGAGAATAGCAACCCTAGAGTGAAAGAGTTAGAAATGGGTGTTCATCCTGAGGGTTTGTCTTACTACTACCTTGATCCTCAAGGGGTGACCCAAGGGCCATATCAGGGGTTAGACATCATTTCATGGTTTGAACAAGGTTTCTTTGGGATAGACTTGCTTGTTCGCTTGGAGGATGCTCCTGAAGGAACACCTTTCAAAGAGTTGGGGGAGTTCATGCCATATCTTAAATCTTGGGATGAGAATGGCAGTATCATAAATCCAAGTTCTAAGCTAGAAGAATCTGGTGGCTTAGGAGGGAGTCTGGAGTCCAGTTTACCCTTTTCTGCAGCAGTTTCAGACAGTAACTATGCATTTCTGGGAAATGACCATCAGCGGCCCATGCCTGAACTAAACAGCCTTTCATCTCAGCATATTCAGCCAAGAATTTCCGAGCCTGAAGCTCCACTACAACTACATTCAAGGGGTCAAAGCTTTAATGATTTTGTTGAACAGGATGAAGATATTGTGTTTCCTGGAATACATGGAACCGCTGCCTATTCTACTGCACGATCTTCTGGGAGCATTCACGATTCTATGGCAAACTCTATTAATCACCTTCCTCCCCCAACTGAAATGACAGAATCTGGTGTGCCTATTCAGAATGATAATAAGTTGCATCCCTTTGGCTTGCTATGGTCTGAGCTAGAAAGCAGCCAATCAAAGCATTCCAAGTCGGCCAATATGCCATCTAGTAAGGGGAGGGCTGTTCCATTTGGTGCAAATCCTGATCCAGCAATTGCTGAGACGTGGTCTGATCTTCATAGAAAAAGTTCAGTCTCTGATCCGAACTTGTATCAGGAAATGTTGACTCCTCGCCAATTGTCGCACATGGAACAGGAACCCAGTCATTATGATTTAGCAGAGCAGCTTATGTCGCAGCAAATTCGACAGCAGCAGCAACTCCAACAGCGAAATATGTTGTCTGGTTTTGCACACTTGAATGACAATGTATTAGATCCTTTGCAAAATCAGAATCTTATTCACCATCAACAGTTAGCCAACCATTCTGCAGCAGATCTGGATCACCTTTTGGCACTTCAGAGGCAGGCTCAGCTCGAGCAACATCAACTGCAACAACAGCAGCAGTTTCATCAACAGCAGAAGCTTTTACAGGAGCAACAGCAATCACAAGTACAACAGGTGCTTTTTGAACAATTGTTGCGTGGTCAAATGCATGATCCTACACTTAGGCAGCCACATGTTGATCCTGTAAGAGCAAACAATGTTATTGATCAGGTTCTATTAGAGCAGCAGCTTCTACGTGAACTTCAGCAACGGTCTCACCATCTTCCAAGGCATGTTGATCCAACTATAGAGCAGTTAATTCAAGCAAAGTTTGGTCATACACCTCAAGGACATCAAACAGATTTGTTTGAGCTACTGTCTCGTGCACAGCATGAACAGATTCAGTCGCTGGAACATCAGATGCATGCCAGGCAGTTACCCATGGGAATAAGGCAGCGAATGGAAGAAGAGAGGCACATTGGTTCTGTCTGGCCAGCTGAGGAATCAAATCAGATTTTCAGGACCCATGCTGGTAATCATGGTCACCGAGGGCACTCTTCTGGCTTTAATCCTTTAGATTTTTATCAGAGGCAGCAGAGGCCATCTCATGAGGAGCAGCTGAATCACCTTGACCGGAATCTTTCATTACAGGATCGACTTCAGCAAGGTTTTTATGAGCCTGGCTCACTGCCGTTTGAGCGGTCAATGTCATTACCTGCTGGTGCACCAGGGATGAACTTGGATGTAGTAAATGCTATGGCTCGTGCCCAGGGTTTAGATATGCAGGATTCCATTGGACGTATGCAGTCGGCTGGCCAGTCGGGTCAATTTTCGTCTGGAATCCCTTCCCACAATGCTCATCATCCTCATGTTCCTAACCAATTTCATGTTTCACATTTGGATGCAATTGAGGGACACTGGCCTGAGAAGAATGATCAGCTTGAGAATGACTGGATGGATGCTCGGTTTCAGCAGCTGCATATTAATGCTGAGAGGCAGAAAAGGGAATCAGAGATCAAGAATACTTCTCAGGATCAAAATTTATGGATGTCAGATGGAATTAATGATGAGAACTCAAAGAGACTACTAATGGATTTACTCCATCAAAAATCTGGCCACCAACCTAGCGAGTCGTTGAATGCGATGAGCAATGGGATGTTCTCTGATAAAAGACTGCCATCTGGCCACTACTCTGGATCAAGCTCCTCAAACCACCTGTTCAATCTGCATGCAGACCAAGAAGCAGGTCTAAATAACTCATTTCGTGTAGGGTCGTTTGGTTCTAATCCAGGTGAACTCCTACAGGAAGAGCAGGCCAGCAGTGTGGAAAGCAATGAAAAGTTGATGTATGGATCTAATTCTGGAGCATTGGCTGATAGAGAGTCATTTCTGGCAGGAATGAATGCAACGAGCCAGTCACTTTACACAAATTCTAACATGATAAGTAAGTCTTCTATAGGTAAAGAACTTTCTGAGTTGGAGGGCAGGAAGCGGGGGTCCAAAAGTGAGGGGATAATTATGGGTCAATCTTTTGAGACTCAAGAACGAATGGTTGAACAAGCAGGGTTGTCTGCAACAAATTTTGAGGAGATGTCAAAACACGCCCATAGCATGAATTCTTCCTCCGGTGTTTCAGGTGGGAATGCTGTTTTTTATGGTGACAAGATTGGCGGAAGTAATTCATTTGTCGAACAAACTGCCAAGGATCGGCGAGACGCAGTGGTTAATCCAGTAAACCAAGGTTCTGATGTCATGGCATCTTCAAAAAAAGAAATGCATTTCCGCCGTTCATCATCCTCCAGTGATGCTGATGTATCAGAGGCATCATTTATCGATATGCTTAAGAGTAATACCAAGAAGATTGCTCCCATGGATGCCCACGCCACAGCTGGATTTCCAGAATCATCAGAAGCAATGCAAGGTGGCCGGAGTGGCAAAAAGAAAGGAAAGAAAGGGAGGCAAATTGATCCTGCCCTCCTTGGTTTTAAAGTCACCAGCAATCGTATAATGATGGGTGAAATTCAACGAATCGACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1508

Amino Acids

168.33

Weight (kDa)

5.48

Isoelectric Point (pI)

54.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 502 - 547 6.3e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 322, 3211, 3722
AccBSI CCGCTC 1 cut(s) 3187
AccI GTMKAC 1 cut(s) 960
AciI CCGC 9 cut(s) 5, 176, 274, 1816, 1971, 3187, 3979, 4158, 4270
AcoI YGGCCR 6 cut(s) 2157, 2978, 3301, 3601, 3670, 4411
AcsI RAATTY 9 cut(s) 680, 1212, 1866, 2364, 3526, 3916, 4066, 4102, 4506
AcuI CTGAAG 7 cut(s) 468, 1212, 1644, 1899, 2504, 2735, 3131
AfaI GTAC 1 cut(s) 2616
AfiI CCNNNNNNNGG 3 cut(s) 648, 1547, 4194
AflII CTTAAG 1 cut(s) 4328
AflIII ACRYGT 1 cut(s) 2680
AjiI CACGTC 1 cut(s) 2229
AleI CACNNNNGTG 1 cut(s) 178
AloI GAACNNNNNNTCC 2 cut(s) 1111, 1143
Alw21I GWGCWC 2 cut(s) 2875, 3214
Alw44I GTGCAC 2 cut(s) 2871, 3210
AlwNI CAGNNNCTG 5 cut(s) 557, 2066, 2261, 2896, 3463
Ama87I CYCGRG 3 cut(s) 570, 760, 2537
ApaLI GTGCAC 2 cut(s) 2871, 3210
ApoI RAATTY 9 cut(s) 680, 1212, 1866, 2364, 3526, 3916, 4066, 4102, 4506
ArsI GACNNNNNNTTYG 2 cut(s) 1346, 1378
AseI ATTAAT 3 cut(s) 2031, 3471, 3549
Asp700I GAANNNNTTC 4 cut(s) 2251, 2886, 3331, 4165
AspS9I GGNCC 9 cut(s) 66, 224, 443, 841, 1201, 1538, 1818, 3009, 3984
AsuII TTCGAA 1 cut(s) 678
AvaI CYCGRG 3 cut(s) 570, 760, 2537
AvaII GGWCC 6 cut(s) 66, 224, 443, 1201, 3009, 3984
AxyI CCTNAGG 1 cut(s) 1491
BaeGI GKGCMC 4 cut(s) 2875, 3042, 3214, 3256
BalI TGGCCA 4 cut(s) 2980, 3303, 3603, 3672
BanII GRGCYC 1 cut(s) 468
BarI GAAGNNNNNNTAC 2 cut(s) 1872, 1904
BauI CACGAG 2 cut(s) 2868, 3249
BbsI GAAGAC 2 cut(s) 456, 3930
Bbv12I GWGCWC 2 cut(s) 2875, 3214
BbvCI CCTCAGC 1 cut(s) 2985
BceAI ACGGC 4 cut(s) 321, 428, 3163, 4257
BcgI CGANNNNNNTGC 4 cut(s) 2348, 2382, 4166, 4200
BclI TGATCA 2 cut(s) 2713, 3421
BfaI CTAG 9 cut(s) 366, 435, 654, 1071, 1458, 1715, 2126, 2172, 3612
BfmI CTRYAG 5 cut(s) 1767, 2490, 2793, 3788, 3942
BfrI CTTAAG 1 cut(s) 4328
BfuAI ACCTGC 3 cut(s) 322, 3211, 3722
BglI GCCNNNNNGGC 1 cut(s) 4419
BglII AGATCT 1 cut(s) 2497
BlpI GCTNAGC 1 cut(s) 2532
Bme18I GGWCC 6 cut(s) 66, 224, 443, 1201, 3009, 3984
BmeT110I CYCGRG 3 cut(s) 570, 760, 2537
BmgBI CACGTC 1 cut(s) 2229
BmgT120I GGNCC 9 cut(s) 66, 224, 443, 841, 1201, 1538, 1818, 3009, 3984
BmiI GGNNCC 7 cut(s) 444, 465, 843, 1969, 2322, 3011, 3985
BmrI ACTGGG 2 cut(s) 621, 2319
BmuI ACTGGG 2 cut(s) 621, 2319
BpiI GAAGAC 2 cut(s) 456, 3930
BplI GAGNNNNNCTC 8 cut(s) 830, 862, 2515, 2547, 3076, 3108, 3155, 3187
BpmI CTGGAG 4 cut(s) 108, 1766, 3870, 4270
Bpu10I CCTNAGC 3 cut(s) 917, 1732, 2985
Bpu1102I GCTNAGC 1 cut(s) 2532
Bpu14I TTCGAA 1 cut(s) 678
BpuEI CTTGAG 6 cut(s) 840, 1047, 1506, 2817, 3449, 4011
Bsa29I ATCGAT 1 cut(s) 4320
BsaAI YACGTR 1 cut(s) 2744
BsaBI GATNNNNATC 3 cut(s) 783, 2502, 3069
BsaI GGTCTC 1 cut(s) 2766
BsaWI WCCGGW 2 cut(s) 3120, 4112
Bsc4I CCNNNNNNNGG 3 cut(s) 648, 1547, 4194
Bse1I ACTGG 9 cut(s) 616, 1752, 2325, 3304, 3410, 3443, 3901, 4217, 4287
Bse21I CCTNAGG 1 cut(s) 1491
Bse3DI GCAATG 4 cut(s) 1312, 3643, 3826, 4407
Bse8I GATNNNNATC 3 cut(s) 783, 2502, 3069
BseCI ATCGAT 1 cut(s) 4320
BseJI GATNNNNATC 3 cut(s) 783, 2502, 3069
BseLI CCNNNNNNNGG 3 cut(s) 648, 1547, 4194
BseMI GCAATG 4 cut(s) 1312, 3643, 3826, 4407
BseNI ACTGG 9 cut(s) 616, 1752, 2325, 3304, 3410, 3443, 3901, 4217, 4287
BseRI GAGGAG 8 cut(s) 26, 31, 851, 920, 2283, 3113, 3682, 4088
BseSI GKGCMC 4 cut(s) 2875, 3042, 3214, 3256
BseYI CCCAGC 1 cut(s) 1036
BsgI GTGCAG 2 cut(s) 622, 1971
BshVI ATCGAT 1 cut(s) 4320
BsiHKAI GWGCWC 2 cut(s) 2875, 3214
BsiHKCI CYCGRG 3 cut(s) 570, 760, 2537
BsiSI CCGG 3 cut(s) 3121, 4113, 4414
BslFI GGGAC 3 cut(s) 456, 1123, 3414
BslI CCNNNNNNNGG 3 cut(s) 648, 1547, 4194
BsmBI CGTCTC 2 cut(s) 2219, 4194
BsmFI GGGAC 3 cut(s) 456, 1123, 3414
BsmI GAATGC 5 cut(s) 2004, 2146, 3631, 3895, 4135
Bso31I GGTCTC 1 cut(s) 2766
BsoBI CYCGRG 3 cut(s) 570, 760, 2537
Bsp119I TTCGAA 1 cut(s) 678
Bsp1286I GDGCHC 5 cut(s) 468, 2875, 3042, 3214, 3256
Bsp1720I GCTNAGC 1 cut(s) 2532
Bsp19I CCATGG 2 cut(s) 2928, 4356
BspACI CCGC 9 cut(s) 5, 176, 274, 1816, 1971, 3187, 3979, 4158, 4270
BspDI ATCGAT 1 cut(s) 4320
BspHI TCATGA 1 cut(s) 3094
BspLI GGNNCC 7 cut(s) 444, 465, 843, 1969, 2322, 3011, 3985
BspMAI CTGCAG 2 cut(s) 1771, 2494
BspMI ACCTGC 3 cut(s) 322, 3211, 3722
BspQI GCTCTTC 1 cut(s) 3789
BspT104I TTCGAA 1 cut(s) 678
BspTI CTTAAG 1 cut(s) 4328
BspTNI GGTCTC 1 cut(s) 2766
BsrBI CCGCTC 1 cut(s) 3187
BsrDI GCAATG 4 cut(s) 1312, 3643, 3826, 4407
BsrI ACTGG 9 cut(s) 616, 1752, 2325, 3304, 3410, 3443, 3901, 4217, 4287
BssNAI GTATAC 1 cut(s) 961
BssSI CACGAG 2 cut(s) 2868, 3249
BssT1I CCWWGG 9 cut(s) 150, 731, 1534, 2774, 2928, 4188, 4225, 4356, 4465
Bst1107I GTATAC 1 cut(s) 961
Bst2BI CACGAG 2 cut(s) 2868, 3249
Bst6I CTCTTC 7 cut(s) 345, 379, 473, 1435, 2949, 3049, 3789
BstAFI CTTAAG 1 cut(s) 4328
BstBAI YACGTR 1 cut(s) 2744
BstBI TTCGAA 1 cut(s) 678
BstDSI CCRYGG 2 cut(s) 2928, 4356
BstEII GGTNACC 2 cut(s) 1528, 3029
BstNSI RCATGY 4 cut(s) 2684, 2783, 2915, 3719
BstPI GGTNACC 2 cut(s) 1528, 3029
BstSFI CTRYAG 5 cut(s) 1767, 2490, 2793, 3788, 3942
BstSLI GKGCMC 4 cut(s) 2875, 3042, 3214, 3256
BstV2I GAAGAC 2 cut(s) 456, 3930
BstX2I RGATCY 6 cut(s) 139, 284, 1385, 2437, 2497, 3839
BstXI CCANNNNNNTGG 2 cut(s) 3680, 3811
BstYI RGATCY 6 cut(s) 139, 284, 1385, 2437, 2497, 3839
BstZ17I GTATAC 1 cut(s) 961
Bsu15I ATCGAT 1 cut(s) 4320
Bsu36I CCTNAGG 1 cut(s) 1491
BsuTUI ATCGAT 1 cut(s) 4320
BtgI CCRYGG 2 cut(s) 2928, 4356
BtgZI GCGATG 1 cut(s) 3644
BtrI CACGTC 1 cut(s) 2229
BtsI GCAGTG 4 cut(s) 185, 3173, 3814, 4212
BtsIMutI CAGTG 8 cut(s) 185, 246, 523, 3173, 3403, 3814, 4212, 4294
BveI ACCTGC 3 cut(s) 322, 3211, 3722
CaiI CAGNNNCTG 5 cut(s) 557, 2066, 2261, 2896, 3463
CciI TCATGA 1 cut(s) 3094
Cfr13I GGNCC 9 cut(s) 66, 224, 443, 841, 1201, 1538, 1818, 3009, 3984
ClaI ATCGAT 1 cut(s) 4320
CseI GACGC 1 cut(s) 4211
Csp6I GTAC 1 cut(s) 2615
CviQI GTAC 1 cut(s) 2615
DraI TTTAAA 1 cut(s) 4474
EaeI YGGCCR 6 cut(s) 2157, 2978, 3301, 3601, 3670, 4411
Eam1104I CTCTTC 7 cut(s) 345, 379, 473, 1435, 2949, 3049, 3789
EarI CTCTTC 7 cut(s) 345, 379, 473, 1435, 2949, 3049, 3789
EciI GGCGGA 3 cut(s) 20, 4173, 4259
Eco130I CCWWGG 9 cut(s) 150, 731, 1534, 2774, 2928, 4188, 4225, 4356, 4465
Eco24I GRGCYC 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 2766
Eco47I GGWCC 6 cut(s) 66, 224, 443, 1201, 3009, 3984
Eco57I CTGAAG 7 cut(s) 468, 1212, 1644, 1899, 2504, 2735, 3131
Eco81I CCTNAGG 1 cut(s) 1491
Eco88I CYCGRG 3 cut(s) 570, 760, 2537
Eco91I GGTNACC 2 cut(s) 1528, 3029
EcoO109I RGGNCCY 4 cut(s) 443, 841, 1201, 3009
EcoO65I GGTNACC 2 cut(s) 1528, 3029
EcoRI GAATTC 1 cut(s) 4102
EcoT14I CCWWGG 9 cut(s) 150, 731, 1534, 2774, 2928, 4188, 4225, 4356, 4465
EcoT22I ATGCAT 4 cut(s) 1795, 2658, 2913, 4266
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 9 cut(s) 150, 731, 1534, 2774, 2928, 4188, 4225, 4356, 4465
Esp3I CGTCTC 2 cut(s) 2219, 4194
FalI AAGNNNNNCTT 2 cut(s) 3922, 3954
FaqI GGGAC 3 cut(s) 456, 1123, 3414
FauI CCCGC 1 cut(s) 3972
FbaI TGATCA 2 cut(s) 2713, 3421
FblI GTMKAC 1 cut(s) 960
FriOI GRGCYC 1 cut(s) 468
FspBI CTAG 9 cut(s) 366, 435, 654, 1071, 1458, 1715, 2126, 2172, 3612
GsaI CCCAGC 1 cut(s) 1040
GsuI CTGGAG 4 cut(s) 108, 1766, 3870, 4270
HapII CCGG 3 cut(s) 3121, 4113, 4414
HgaI GACGC 1 cut(s) 4211
HincII GTYRAC 1 cut(s) 2289
HindII GTYRAC 1 cut(s) 2289
HindIII AAGCTT 3 cut(s) 770, 1912, 2585
HpaII CCGG 3 cut(s) 3121, 4113, 4414
Hpy166II GTNNAC 9 cut(s) 961, 1130, 1757, 2289, 2747, 2873, 3212, 3782, 4222
Hpy8I GTNNAC 9 cut(s) 961, 1130, 1757, 2289, 2747, 2873, 3212, 3782, 4222
Hpy99I CGWCG 4 cut(s) 32, 342, 1403, 4523
HpyCH4IV ACGT 4 cut(s) 1401, 2228, 2743, 3286
HpySE526I ACGT 4 cut(s) 1401, 2228, 2743, 3286
Ksp22I TGATCA 2 cut(s) 2713, 3421
LguI GCTCTTC 1 cut(s) 3789
MaeI CTAG 9 cut(s) 366, 435, 654, 1071, 1458, 1715, 2126, 2172, 3612
MaeII ACGT 4 cut(s) 1401, 2228, 2743, 3286
MbiI CCGCTC 1 cut(s) 3187
MfeI CAATTG 4 cut(s) 2217, 2300, 2636, 3393
MflI RGATCY 6 cut(s) 139, 284, 1385, 2437, 2497, 3839
MhlI GDGCHC 5 cut(s) 468, 2875, 3042, 3214, 3256
MlsI TGGCCA 4 cut(s) 2980, 3303, 3603, 3672
MluNI TGGCCA 4 cut(s) 2980, 3303, 3603, 3672
MlyI GAGTC 8 cut(s) 212, 569, 1750, 1757, 2284, 3626, 3878, 4018
MmeI TCCRAC 7 cut(s) 192, 251, 258, 1413, 2410, 2813, 3945
Mox20I TGGCCA 4 cut(s) 2980, 3303, 3603, 3672
Mph1103I ATGCAT 4 cut(s) 1795, 2658, 2913, 4266
MroXI GAANNNNTTC 4 cut(s) 2251, 2886, 3331, 4165
MscI TGGCCA 4 cut(s) 2980, 3303, 3603, 3672
MslI CAYNNNNRTG 6 cut(s) 178, 1299, 2421, 3354, 3387, 3471
Msp20I TGGCCA 4 cut(s) 2980, 3303, 3603, 3672
MspA1I CMGCKG 8 cut(s) 554, 743, 1816, 1973, 2984, 3106, 3463, 4376
MspCI CTTAAG 1 cut(s) 4328
MspI CCGG 3 cut(s) 3121, 4113, 4414
MunI CAATTG 4 cut(s) 2217, 2300, 2636, 3393
Mva1269I GAATGC 5 cut(s) 2004, 2146, 3631, 3895, 4135
NcoI CCATGG 2 cut(s) 2928, 4356
NlaIV GGNNCC 7 cut(s) 444, 465, 843, 1969, 2322, 3011, 3985
NmuCI GTSAC 8 cut(s) 171, 518, 1057, 1528, 3029, 3903, 4145, 4477
NsiI ATGCAT 4 cut(s) 1795, 2658, 2913, 4266
NspI RCATGY 4 cut(s) 2684, 2783, 2915, 3719
NspV TTCGAA 1 cut(s) 678
OliI CACNNNNGTG 1 cut(s) 178
PaeI GCATGC 2 cut(s) 2915, 3719
PaeR7I CTCGAG 1 cut(s) 2537
PagI TCATGA 1 cut(s) 3094
PasI CCCWGGG 2 cut(s) 695, 3256
PciI ACATGT 1 cut(s) 2680
PciSI GCTCTTC 1 cut(s) 3789
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 412, 3626
PctI GAATGC 5 cut(s) 2004, 2146, 3631, 3895, 4135
PdmI GAANNNNTTC 4 cut(s) 2251, 2886, 3331, 4165
PflFI GACNNNGTC 1 cut(s) 2230
PfoI TCCNGGA 2 cut(s) 62, 1954
PleI GAGTC 8 cut(s) 211, 568, 1749, 1756, 2284, 3625, 3877, 4018
PpsI GAGTC 8 cut(s) 211, 568, 1749, 1756, 2284, 3625, 3877, 4018
Ppu21I YACGTR 1 cut(s) 2744
PpuMI RGGWCCY 3 cut(s) 443, 1201, 3009
PscI ACATGT 1 cut(s) 2680
PshBI ATTAAT 3 cut(s) 2031, 3471, 3549
Psp5II RGGWCCY 3 cut(s) 443, 1201, 3009
PspEI GGTNACC 2 cut(s) 1528, 3029
PspFI CCCAGC 1 cut(s) 1036
PspN4I GGNNCC 7 cut(s) 444, 465, 843, 1969, 2322, 3011, 3985
PspPI GGNCC 9 cut(s) 66, 224, 443, 841, 1201, 1538, 1818, 3009, 3984
PspPPI RGGWCCY 3 cut(s) 443, 1201, 3009
PspXI VCTCGAGB 1 cut(s) 2537
PstI CTGCAG 2 cut(s) 1771, 2494
PstNI CAGNNNCTG 5 cut(s) 557, 2066, 2261, 2896, 3463
PsuI RGATCY 6 cut(s) 139, 284, 1385, 2437, 2497, 3839
PsyI GACNNNGTC 1 cut(s) 2230
PvuII CAGCTG 6 cut(s) 554, 743, 2984, 3106, 3463, 4376
RsaI GTAC 1 cut(s) 2616
RsaNI GTAC 1 cut(s) 2615
RseI CAYNNNNRTG 6 cut(s) 178, 1299, 2421, 3354, 3387, 3471
SapI GCTCTTC 1 cut(s) 3789
Sau96I GGNCC 9 cut(s) 66, 224, 443, 841, 1201, 1538, 1818, 3009, 3984
SchI GAGTC 8 cut(s) 212, 569, 1750, 1757, 2284, 3626, 3878, 4018
SduI GDGCHC 5 cut(s) 468, 2875, 3042, 3214, 3256
SfcI CTRYAG 5 cut(s) 1767, 2490, 2793, 3788, 3942
Sfr274I CTCGAG 1 cut(s) 2537
SfuI TTCGAA 1 cut(s) 678
SinI GGWCC 6 cut(s) 66, 224, 443, 1201, 3009, 3984
SlaI CTCGAG 1 cut(s) 2537
SmiMI CAYNNNNRTG 6 cut(s) 178, 1299, 2421, 3354, 3387, 3471
SmlI CTYRAG 8 cut(s) 855, 1062, 1521, 2537, 2832, 3428, 4026, 4328
SmoI CTYRAG 8 cut(s) 855, 1062, 1521, 2537, 2832, 3428, 4026, 4328
SphI GCATGC 2 cut(s) 2915, 3719
SsiI CCGC 9 cut(s) 5, 176, 274, 1816, 1971, 3187, 3979, 4158, 4270
SspI AATATT 1 cut(s) 997
SspMI CTAG 9 cut(s) 366, 435, 654, 1071, 1458, 1715, 2126, 2172, 3612
StyI CCWWGG 9 cut(s) 150, 731, 1534, 2774, 2928, 4188, 4225, 4356, 4465
TaiI ACGT 4 cut(s) 1404, 2231, 2746, 3289
TaqI TCGA 9 cut(s) 18, 678, 1052, 2368, 2538, 3142, 4176, 4320, 4518
TatI WGTACW 1 cut(s) 2614
TauI GCSGC 1 cut(s) 1819
TscAI CASTG 8 cut(s) 185, 253, 523, 3180, 3410, 3814, 4212, 4294
TseFI GTSAC 8 cut(s) 171, 518, 1057, 1528, 3029, 3903, 4145, 4477
Tsp45I GTSAC 8 cut(s) 171, 518, 1057, 1528, 3029, 3903, 4145, 4477
TspGWI ACGGA 1 cut(s) 621
TspRI CASTG 8 cut(s) 185, 253, 523, 3180, 3410, 3814, 4212, 4294
Tth111I GACNNNGTC 1 cut(s) 2230
Vha464I CTTAAG 1 cut(s) 4328
VneI GTGCAC 2 cut(s) 2871, 3210
VpaK11BI GGWCC 6 cut(s) 66, 224, 443, 1201, 3009, 3984
VspI ATTAAT 3 cut(s) 2031, 3471, 3549
XapI RAATTY 9 cut(s) 680, 1212, 1866, 2364, 3526, 3916, 4066, 4102, 4506
XceI RCATGY 4 cut(s) 2684, 2783, 2915, 3719
XcmI CCANNNNNNNNNTGG 2 cut(s) 3596, 4373
XhoI CTCGAG 1 cut(s) 2537
XmiI GTMKAC 1 cut(s) 960
XmnI GAANNNNTTC 4 cut(s) 2251, 2886, 3331, 4165
XspI CTAG 9 cut(s) 366, 435, 654, 1071, 1458, 1715, 2126, 2172, 3612
Zsp2I ATGCAT 4 cut(s) 1795, 2658, 2913, 4266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.