Rroxscaffold_5G00371320

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005720
Physical Location & Seq
Forward (+)
52917486 .. 52924489
7004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371320.1

Sequence Viewer

Length: 4749 bp
ATGGCGGAACGCAAGCTCGATCTCCCCGACGATCTCCTCTCCTGCAAACCCTCCGATCAGTCCTGGACCTCCAAAGTTGAAGTTTCTGGAGGAAATGATGAGGAAAAGGTGCTTTTGGGGTCATCCGATGAGTTGAAAGATCCAGCAGCCTTGGATAGCAGCATACCTTTGTCACCCCAGTGGCTTTATGCTAAACCGATTGAGTCTAAGTTGGAAATGCGTGGTCCAACTTCACTCGGAAACTCCTCTGACAGCAACCAGAAGGACGGTTGGCGGTTGGAAGGATCTGAGGACAAGAAAGATTGGAGGCGACCTGCTACTGAAAGTGAAAGCAGCCGTCGTTGGCGTGAAGAGGAAAGAGAAACTAGCTTGCTTGGTGGTAGAAGAGATCGTAGGAAAGCAGAACGCCGTGCTGACAATATTCCATTGAGGGACACAACTGACAGTAGAGCATTGCCTACAACTGACAGGTGGCATGATGGCCGCAATGAAGTACGGCGTGATAGCAAATGGTCGTCTAGGTGGGGTCCTGAAGACAAAGAAAAGGAGCCCCGAACTGAGAAGAGAACAGATGTGGAGAAGGATGATGCTCACAGTAACAGTGAGAGTCAATCTCTTGGAGCTAACAACCGTTCAGCTGCTGAGCGTGAGTCAGATTCTCGGGATAAATGGAGGCCACGACATAGAATGGAGGTTCAAACTGGGGGGTCAGCTACTTACCGTGCTGCACCTGGATTTGGACTAGAAAGAGGACGGGTGGAGGGTTCGAATTTGGGATTTACCCAGGGGCGAGGAAGGTCAAGTGGTATTGGGAGATCCGCAGGCACTATTGGCTCTGCTCTTTCAGGAAAAAGTGGAGGTGTCCCTGGGAAACCAAGGCACTCAGCTGATGGTTTTTGCTACCCGAGGGGAAAGCTTCTTGATATTTATCGGCAGCGAAAGCCTGAACTATCTTTTGATACCATGCCAGATGAGATGGAGGAGGCCCCCTCACTAACTCAAGTTGATTTTGTTGAACCATTGGCTTTTCATGCCCCTGATGCTGATGAGGAGGCCATCCTTAGCGATATATGGAAGGGAAAAATTACAAGTAGTGGGGTAGTATACAATTCATTCAGAAAAGGGAGATCAACTGAAAATATTACAGGTGTGGGAGACTTAGAAGCTGCTGATGGAGTTCTGGGCAATTTGCCCTCGACTGTCACTCAAGAGACTAGCACCTTTCTGGAGGCTGCAAATGCTGATGACTATGGGACTTTGTGGAATTATGGTTCACAGAGGAATGTGATAAATGGAAAAGATGTTGGTCATAAAGAAAGTGATAACAGAGCTACTGAAGGAAAGGACCTTGATGGAATTTCCCTATCAATTCAAAAAAGTAATGGTATCTATGGTGATGTTGAGACTGATGGTTCTTATGATAGTGCAAATCAGCTTCATGGCGGTGGCAGTAGGAAAATTGGAGATTCTGCCTTTTCAAATCATCCTGTATCTGATGACATTGAGTTTGCCAATTATTCCGAAATAAGATCCAACCTTCCCGACGTTTCAAATACTCTCTATGGTTTGGCCTCTTCTGAGCAGAATGAGAATAGCAACCCTGTAGTGAAAGAGTTAGAAATGGGTGTTCATCCTGAGGGTTTGTATTACTACTACCTTGATCCTCAAGGGGTGACCCAGGGGCCATATCAGGGGTTAGACATCATTTCATGGTTTGAACAAGGTTTCTTTGGGATAGACTTGCTTGTTCGCTTGGAGGATGCTCCTGAAGGAACACCGTTCAAAGAGTTGGGGGAGTTCATGCCATATCTTAAATCTTGGGGTGAGAATGGCAGTATCATAAATCCAAGTTCCAAGCTAGAAGAATCTGGTGGCTTAGGAGGGAGTCTGGAGTCCAGTTTACCCTTTTCTGCAGCAGTTTCAGACAGTAACTATGCATTTCTGGGAAATGACCATCAGCGGCCCATGTCTGAACTAAACAGCCTTTCAGCTCAGCATATTCAGCCAAGAATTTCCGAGCCTGAAGCTCCACTACAACTACATACAAGGGGTCAAAGCTTTAATGATTTTGTTGAACAAGATGAAGATATTGTGTTTCCTGGAATACATGGAACCGCTGCCTATTCTACTGCACGATCTTCTGGGAGCATTCACGATTCTATGGCAAACTCGATTAATCACCTTCCTCCCCCAACTGAATTGACAGAATCTGGTGTGCCTATTCAGAATGATAATAAGTTGCATCCCTTTGGCTTGCTATGGTCTGAGCTAGAAAGTGGCCAATCAAAGCATTCCAAGTCGGCCAATATGCCATCTAGTAAGGGGAGGGCTGTTCCATTTGGTGCAAATCCTGATCCAGCAATTGCTGAGACGTGGTCTGATCTTCATAGAAAAAGTTCAGCCTCTGATCCAAACTTGTATCAGGAAATGTTGACTCCTCGCCAATTGTCGCACATGGAACAGGAATCCAGTCATTATGATTTAGCAGAGCAGCTTATGTCGCAGCAAATTCGACAGCAGCAGCAACTCCAACAGCGAAATATGTTGTCTGGTTTTGCACACTTGAATGACAATGTTTTAGATCCTTTGCAAAATCAGAATCTTATTCACCATCAACAGTTAGCCAACCATTCTGCAGCAGATCTGGATCACCTTTTGGCACTTCAGAGGCAGGCTCAGCTCGAGCAACATCAACTGCAACAACAGCAGCAGTTTCATCAACAGCAGAAGCTTTTGCAGGAGCAACAGCAATCTCAAGTACAACAGGTGCTTTTTGAACAATTGTTGCGTGGTCAAATGCATGATCCTACACTTAGGCAGCCACATGTTGATCCTGTAAGAGCAAACAATGTTATTGATCAGGTTCTGTTAGAGCAGCAGCTTCTACGTGAACTTCAGCAACGGTCTCACCATCTTCCAAGGCATGTTGATCCAACTATGGAGCAGTTAATTCAAGCAAAGTTTGGTCATACACCAGGACATCAAACAGATTTATTTGAGCTACTGTCTCGTGCACAGCATGAACAGATTCAGTCGCTGGAACATCAGATGCATGCCAGGCAGTTACCCATGGGAATAAGGCAGCGAATAGAAGAAGAGAGGCATATCGGTTCTGTCTGGCCAGCTGAGGAATCAAATCAGATTTTCAGGACCCATGCTGGTAATCATGGTCACCGAGGGCACTCTTCTGGCTTTAATCCTTTAGATTTTTATCAGAGGCAGCAGAGGCCATCTCATGAGGAGCAGCTGAATCACCTTGACCGGAATCTTTCGGTACAGGATCGACTTCAGCAAGGTTTTTATGAGCCTGGCTCGCTGTCGTTTGAGAGGTCAATGTCATTACCTGCTGGTGCACCAGGGATGAACTTGGATGTAGTAAATGCTATGGCTCGTGCCCAGGGTTTAGATATGCAGGATTCCATTGGACGTATGCAGTCGGCTGGCCAGTCGGGTCAATTTTCGTCTGGAATCCCTTCCCACAATGCTCATCATCCTCATGTTCCTAACCAATTTCATGTTTCACATTTGGATGCAATTGAGGGACACTGGCCTGAGAAGAATGATCAGCTTGAGAATGACTGGATGGACACTCGGTTTCAGCAGCTGCATATTAATGCTGAGAGGCAGAAAAGGGAATCAGAGATCAAGAATACTTCTCAGGATCAAAATTTATGGATGTCAGATGGAATTAATGATGAGAACTCAAAGAGACTACTAATGGATTTACTCCATCAAAAATCTGGCCATCAACCTAGCGAGTCGTTGAATGCGATGAGCAATGGGATGTTCTCTGATAAAAGACTGCCATCTGGCCACTACTCTGGATCAAGCTCCACAAACCACCTGTTCAATCTCCATGCAGACCAAGAAGCAGGTCTAAATAACTCATTTCGTGTAGGGTCATTTGGTTCTAATCCAGGTGAACTCCTACAGGAAGAGCAGGCCAGCAGTGTGGAAAGCAATGAAAAGTTGATGTATAGATCTAATTCTGGAGCATTGGCTGATAGAGAGTCATTTCTGGCAGGAATGAATGCAACGAGTCAGTCGCTTTACACAAATTCTAACATGATAAGCAAGTCTTCTATAGGTAAAGAACTTTCTGAGTTGGAGGGCAGGAAGCGGGGGTCCAAAAGTGAGGGGATAATTATGGGTCGATCTTTTGAGACTCAAGAACGAATGGTTGAACAAGCAGGGTTGTCTGCAACAAATTTTGAGGAGAGGTCAAACAACGCCCATAGCATGAATTCTTCCTCCGGTGTTTCAGGTGGGAATGCTGGTTTCTATGGTGACAAGATTGGCCGAAGTAATTCATTTGTCGAACAAACTGCCAAGGATCGGGCTCCCATCCCATCCAAAGGGCAAGAAAATATTCTGCTGAGACGACCTTCTGTCCCAAGTGCTTCAGCATCGCAAGAAGGATTATCAGAACTGATATCAGATCCAGTTCTTAGAGGAAAAAATTCAAGTGCTGCTTTAGATGAAATGCATTTCCACCGTACATCATCCGCCAGTGATGCTGATGTATCAGAGGCATCATTTATCGATATGCTTAAGAGTAATACCAAGAAGATTGCTCCCATGGATGCCCACGCCACAGCTGGATTTCCAGAATCATCAGAAGCAATGCAAGGTGGCCGGAGTGGCAAAAAGAAAGGAAAGAAAGGGAGGCAAATTGATCCTGCCCTCCTTGGTTTTAAAGTCACCAGCAATCGTATAATGATGGGTGAAATTCAACGAATCGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1582

Amino Acids

175.69

Weight (kDa)

5.51

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 550 - 595 6.7e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 322, 3352, 3863
AccI GTMKAC 1 cut(s) 1104
AciI CCGC 9 cut(s) 5, 274, 484, 819, 1443, 1960, 2115, 4120, 4506
AcoI YGGCCR 9 cut(s) 481, 2278, 2301, 3119, 3442, 3742, 3811, 4297, 4633
AcuI CTGAAG 8 cut(s) 552, 1356, 1788, 2043, 2648, 2879, 3272, 4386
AfaI GTAC 4 cut(s) 495, 2760, 3276, 4498
AfiI CCNNNNNNNGG 4 cut(s) 737, 1691, 4335, 4355
AflII CTTAAG 1 cut(s) 4550
AflIII ACRYGT 1 cut(s) 2824
AhdI GACNNNNNGTC 1 cut(s) 4388
AjiI CACGTC 1 cut(s) 2373
AleI CACNNNNGTG 1 cut(s) 178
AloI GAACNNNNNNTCC 2 cut(s) 1255, 1287
Alw21I GWGCWC 2 cut(s) 3016, 3355
Alw26I GTCTC 9 cut(s) 1149, 1205, 1397, 2363, 2910, 3012, 3703, 4157, 4372
Alw44I GTGCAC 2 cut(s) 3012, 3351
AlwNI CAGNNNCTG 6 cut(s) 641, 2210, 2405, 2866, 3037, 3604
Ama87I CYCGRG 3 cut(s) 660, 904, 2681
ApaLI GTGCAC 2 cut(s) 3012, 3351
ArsI GACNNNNNNTTYG 2 cut(s) 1490, 1522
AseI ATTAAT 3 cut(s) 2175, 3612, 3690
Asp700I GAANNNNTTC 6 cut(s) 2395, 3027, 3472, 4306, 4368, 4459
AspS9I GGNCC 9 cut(s) 66, 224, 527, 985, 1345, 1682, 1962, 3150, 4125
AsuII TTCGAA 1 cut(s) 767
AvaI CYCGRG 3 cut(s) 660, 904, 2681
AvaII GGWCC 6 cut(s) 66, 224, 527, 1345, 3150, 4125
AxyI CCTNAGG 1 cut(s) 1635
BaeGI GKGCMC 4 cut(s) 3016, 3183, 3355, 3397
BalI TGGCCA 5 cut(s) 2280, 3121, 3444, 3744, 3813
BanII GRGCYC 2 cut(s) 552, 4342
BarI GAAGNNNNNNTAC 2 cut(s) 2016, 2048
BauI CACGAG 2 cut(s) 3009, 3390
BbsI GAAGAC 2 cut(s) 540, 4071
Bbv12I GWGCWC 2 cut(s) 3016, 3355
BbvCI CCTCAGC 1 cut(s) 3126
BceAI ACGGC 3 cut(s) 321, 393, 512
BcgI CGANNNNNNTGC 4 cut(s) 2492, 2526, 4307, 4341
BclI TGATCA 2 cut(s) 2857, 3562
BcoDI GTCTC 9 cut(s) 1149, 1205, 1397, 2363, 2910, 3012, 3703, 4157, 4372
BfaI CTAG 8 cut(s) 366, 519, 743, 1215, 1859, 2270, 2316, 3753
BfmI CTRYAG 5 cut(s) 1602, 1911, 2634, 3929, 4083
BfrI CTTAAG 1 cut(s) 4550
BfuAI ACCTGC 3 cut(s) 322, 3352, 3863
BglI GCCNNNNNGGC 1 cut(s) 4641
BglII AGATCT 2 cut(s) 2641, 3980
BlpI GCTNAGC 3 cut(s) 642, 1992, 2676
Bme18I GGWCC 6 cut(s) 66, 224, 527, 1345, 3150, 4125
BmeRI GACNNNNNGTC 1 cut(s) 4388
BmeT110I CYCGRG 3 cut(s) 660, 904, 2681
BmgBI CACGTC 1 cut(s) 2373
BmgT120I GGNCC 9 cut(s) 66, 224, 527, 985, 1345, 1682, 1962, 3150, 4125
BmiI GGNNCC 8 cut(s) 528, 549, 987, 1683, 2113, 3152, 4126, 4341
BmrI ACTGGG 2 cut(s) 172, 711
BmuI ACTGGG 2 cut(s) 172, 711
BpiI GAAGAC 2 cut(s) 540, 4071
BpmI CTGGAG 4 cut(s) 108, 1247, 1910, 4011
Bpu10I CCTNAGC 3 cut(s) 1061, 1876, 3126
Bpu1102I GCTNAGC 3 cut(s) 642, 1992, 2676
Bpu14I TTCGAA 1 cut(s) 767
BpuEI CTTGAG 6 cut(s) 984, 1191, 1650, 2739, 3590, 4152
Bsa29I ATCGAT 2 cut(s) 4542, 4740
BsaAI YACGTR 1 cut(s) 2888
BsaBI GATNNNNATC 3 cut(s) 927, 2646, 3210
BsaI GGTCTC 1 cut(s) 2910
BsaWI WCCGGW 2 cut(s) 3261, 4253
Bsc4I CCNNNNNNNGG 4 cut(s) 737, 1691, 4335, 4355
Bse1I ACTGG 9 cut(s) 178, 706, 1896, 2469, 3445, 3551, 3584, 4442, 4509
Bse21I CCTNAGG 1 cut(s) 1635
Bse3DI GCAATG 5 cut(s) 452, 493, 3784, 3967, 4629
Bse8I GATNNNNATC 3 cut(s) 927, 2646, 3210
BseCI ATCGAT 2 cut(s) 4542, 4740
BseJI GATNNNNATC 3 cut(s) 927, 2646, 3210
BseLI CCNNNNNNNGG 4 cut(s) 737, 1691, 4335, 4355
BseMI GCAATG 5 cut(s) 452, 493, 3784, 3967, 4629
BseNI ACTGG 9 cut(s) 178, 706, 1896, 2469, 3445, 3551, 3584, 4442, 4509
BseRI GAGGAG 7 cut(s) 26, 235, 995, 1064, 2427, 3254, 4229
BseSI GKGCMC 4 cut(s) 3016, 3183, 3355, 3397
BsgI GTGCAG 2 cut(s) 711, 2115
BshVI ATCGAT 2 cut(s) 4542, 4740
BsiHKAI GWGCWC 2 cut(s) 3016, 3355
BsiHKCI CYCGRG 3 cut(s) 660, 904, 2681
BsiSI CCGG 3 cut(s) 3262, 4254, 4636
BslFI GGGAC 5 cut(s) 446, 848, 1267, 3555, 4376
BslI CCNNNNNNNGG 4 cut(s) 737, 1691, 4335, 4355
BsmAI GTCTC 9 cut(s) 1149, 1205, 1397, 2363, 2910, 3012, 3703, 4157, 4372
BsmBI CGTCTC 2 cut(s) 2363, 4372
BsmFI GGGAC 5 cut(s) 446, 848, 1267, 3555, 4376
BsmI GAATGC 5 cut(s) 2148, 2290, 3772, 4036, 4276
Bso31I GGTCTC 1 cut(s) 2910
BsoBI CYCGRG 3 cut(s) 660, 904, 2681
Bsp119I TTCGAA 1 cut(s) 767
Bsp1286I GDGCHC 6 cut(s) 552, 3016, 3183, 3355, 3397, 4342
Bsp1720I GCTNAGC 3 cut(s) 642, 1992, 2676
Bsp19I CCATGG 2 cut(s) 3069, 4578
BspACI CCGC 9 cut(s) 5, 274, 484, 819, 1443, 1960, 2115, 4120, 4506
BspDI ATCGAT 2 cut(s) 4542, 4740
BspHI TCATGA 1 cut(s) 3235
BspLI GGNNCC 8 cut(s) 528, 549, 987, 1683, 2113, 3152, 4126, 4341
BspMAI CTGCAG 2 cut(s) 1915, 2638
BspMI ACCTGC 3 cut(s) 322, 3352, 3863
BspQI GCTCTTC 1 cut(s) 3930
BspT104I TTCGAA 1 cut(s) 767
BspTI CTTAAG 1 cut(s) 4550
BspTNI GGTCTC 1 cut(s) 2910
BsrDI GCAATG 5 cut(s) 452, 493, 3784, 3967, 4629
BsrI ACTGG 9 cut(s) 178, 706, 1896, 2469, 3445, 3551, 3584, 4442, 4509
BssNAI GTATAC 1 cut(s) 1105
BssSI CACGAG 2 cut(s) 3009, 3390
BssT1I CCWWGG 7 cut(s) 150, 875, 2918, 3069, 4329, 4578, 4687
Bst1107I GTATAC 1 cut(s) 1105
Bst2BI CACGAG 2 cut(s) 3009, 3390
Bst6I CTCTTC 7 cut(s) 345, 379, 557, 1579, 3090, 3190, 3930
BstAFI CTTAAG 1 cut(s) 4550
BstBAI YACGTR 1 cut(s) 2888
BstBI TTCGAA 1 cut(s) 767
BstDSI CCRYGG 2 cut(s) 3069, 4578
BstEII GGTNACC 2 cut(s) 1672, 3170
BstMAI GTCTC 9 cut(s) 1149, 1205, 1397, 2363, 2910, 3012, 3703, 4157, 4372
BstNSI RCATGY 3 cut(s) 2828, 2927, 3056
BstPI GGTNACC 2 cut(s) 1672, 3170
BstSFI CTRYAG 5 cut(s) 1602, 1911, 2634, 3929, 4083
BstSLI GKGCMC 4 cut(s) 3016, 3183, 3355, 3397
BstV2I GAAGAC 2 cut(s) 540, 4071
BstX2I RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3980, 4438
BstXI CCANNNNNNTGG 2 cut(s) 3821, 3952
BstYI RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3980, 4438
BstZ17I GTATAC 1 cut(s) 1105
Bsu15I ATCGAT 2 cut(s) 4542, 4740
Bsu36I CCTNAGG 1 cut(s) 1635
BsuTUI ATCGAT 2 cut(s) 4542, 4740
BtgI CCRYGG 2 cut(s) 3069, 4578
BtgZI GCGATG 2 cut(s) 3785, 4392
BtrI CACGTC 1 cut(s) 2373
BtsI GCAGTG 1 cut(s) 3955
BtsIMutI CAGTG 5 cut(s) 185, 607, 3544, 3955, 4516
BveI ACCTGC 3 cut(s) 322, 3352, 3863
CaiI CAGNNNCTG 6 cut(s) 641, 2210, 2405, 2866, 3037, 3604
CciI TCATGA 1 cut(s) 3235
Cfr13I GGNCC 9 cut(s) 66, 224, 527, 985, 1345, 1682, 1962, 3150, 4125
ClaI ATCGAT 2 cut(s) 4542, 4740
Csp6I GTAC 4 cut(s) 494, 2759, 3275, 4497
CviQI GTAC 4 cut(s) 494, 2759, 3275, 4497
DraI TTTAAA 1 cut(s) 4696
DriI GACNNNNNGTC 1 cut(s) 4388
EaeI YGGCCR 9 cut(s) 481, 2278, 2301, 3119, 3442, 3742, 3811, 4297, 4633
Eam1104I CTCTTC 7 cut(s) 345, 379, 557, 1579, 3090, 3190, 3930
Eam1105I GACNNNNNGTC 1 cut(s) 4388
EarI CTCTTC 7 cut(s) 345, 379, 557, 1579, 3090, 3190, 3930
EciI GGCGGA 2 cut(s) 20, 4495
Eco130I CCWWGG 7 cut(s) 150, 875, 2918, 3069, 4329, 4578, 4687
Eco24I GRGCYC 2 cut(s) 552, 4342
Eco31I GGTCTC 1 cut(s) 2910
Eco32I GATATC 1 cut(s) 4434
Eco47I GGWCC 6 cut(s) 66, 224, 527, 1345, 3150, 4125
Eco57I CTGAAG 8 cut(s) 552, 1356, 1788, 2043, 2648, 2879, 3272, 4386
Eco81I CCTNAGG 1 cut(s) 1635
Eco88I CYCGRG 3 cut(s) 660, 904, 2681
Eco91I GGTNACC 2 cut(s) 1672, 3170
EcoO109I RGGNCCY 4 cut(s) 527, 985, 1345, 3150
EcoO65I GGTNACC 2 cut(s) 1672, 3170
EcoRI GAATTC 1 cut(s) 4243
EcoRV GATATC 1 cut(s) 4434
EcoT14I CCWWGG 7 cut(s) 150, 875, 2918, 3069, 4329, 4578, 4687
EcoT22I ATGCAT 4 cut(s) 1939, 2802, 3054, 4488
EcoT38I GRGCYC 2 cut(s) 552, 4342
ErhI CCWWGG 7 cut(s) 150, 875, 2918, 3069, 4329, 4578, 4687
Esp3I CGTCTC 2 cut(s) 2363, 4372
FalI AAGNNNNNCTT 4 cut(s) 4063, 4095, 4456, 4488
FaqI GGGAC 5 cut(s) 446, 848, 1267, 3555, 4376
FauI CCCGC 1 cut(s) 4113
FbaI TGATCA 2 cut(s) 2857, 3562
FblI GTMKAC 1 cut(s) 1104
FriOI GRGCYC 2 cut(s) 552, 4342
FspBI CTAG 8 cut(s) 366, 519, 743, 1215, 1859, 2270, 2316, 3753
GsuI CTGGAG 4 cut(s) 108, 1247, 1910, 4011
HapII CCGG 3 cut(s) 3262, 4254, 4636
HincII GTYRAC 1 cut(s) 2433
HindII GTYRAC 1 cut(s) 2433
HindIII AAGCTT 3 cut(s) 914, 2056, 2729
HpaII CCGG 3 cut(s) 3262, 4254, 4636
Hpy166II GTNNAC 8 cut(s) 1105, 1274, 1901, 2433, 2891, 3014, 3353, 3923
Hpy8I GTNNAC 8 cut(s) 1105, 1274, 1901, 2433, 2891, 3014, 3353, 3923
Hpy99I CGWCG 3 cut(s) 32, 342, 1547
HpyCH4IV ACGT 4 cut(s) 1545, 2372, 2887, 3427
HpySE526I ACGT 4 cut(s) 1545, 2372, 2887, 3427
Ksp22I TGATCA 2 cut(s) 2857, 3562
LguI GCTCTTC 1 cut(s) 3930
MaeI CTAG 8 cut(s) 366, 519, 743, 1215, 1859, 2270, 2316, 3753
MaeII ACGT 4 cut(s) 1545, 2372, 2887, 3427
MaeIII GTNAC 9 cut(s) 171, 596, 1201, 1672, 1928, 3063, 3170, 4286, 4699
MfeI CAATTG 4 cut(s) 2361, 2444, 2780, 3534
MflI RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3980, 4438
MhlI GDGCHC 6 cut(s) 552, 3016, 3183, 3355, 3397, 4342
MlsI TGGCCA 5 cut(s) 2280, 3121, 3444, 3744, 3813
MluNI TGGCCA 5 cut(s) 2280, 3121, 3444, 3744, 3813
MmeI TCCRAC 7 cut(s) 192, 251, 258, 1557, 2554, 2957, 4086
Mox20I TGGCCA 5 cut(s) 2280, 3121, 3444, 3744, 3813
Mph1103I ATGCAT 4 cut(s) 1939, 2802, 3054, 4488
MroXI GAANNNNTTC 6 cut(s) 2395, 3027, 3472, 4306, 4368, 4459
MscI TGGCCA 5 cut(s) 2280, 3121, 3444, 3744, 3813
MseI TTAA 9 cut(s) 1812, 2061, 2175, 2948, 3195, 3612, 3690, 4551, 4695
MslI CAYNNNNRTG 6 cut(s) 178, 1443, 2565, 3495, 3528, 3612
Msp20I TGGCCA 5 cut(s) 2280, 3121, 3444, 3744, 3813
MspA1I CMGCKG 8 cut(s) 638, 887, 1960, 2117, 3125, 3247, 3604, 4598
MspCI CTTAAG 1 cut(s) 4550
MspI CCGG 3 cut(s) 3262, 4254, 4636
MunI CAATTG 4 cut(s) 2361, 2444, 2780, 3534
Mva1269I GAATGC 5 cut(s) 2148, 2290, 3772, 4036, 4276
NcoI CCATGG 2 cut(s) 3069, 4578
NlaIV GGNNCC 8 cut(s) 528, 549, 987, 1683, 2113, 3152, 4126, 4341
NmuCI GTSAC 6 cut(s) 171, 1201, 1672, 3170, 4286, 4699
NsiI ATGCAT 4 cut(s) 1939, 2802, 3054, 4488
NspI RCATGY 3 cut(s) 2828, 2927, 3056
NspV TTCGAA 1 cut(s) 767
OliI CACNNNNGTG 1 cut(s) 178
PaeI GCATGC 1 cut(s) 3056
PaeR7I CTCGAG 1 cut(s) 2681
PagI TCATGA 1 cut(s) 3235
PasI CCCWGGG 4 cut(s) 784, 866, 1678, 3397
PciI ACATGT 1 cut(s) 2824
PciSI GCTCTTC 1 cut(s) 3930
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 3767
PctI GAATGC 5 cut(s) 2148, 2290, 3772, 4036, 4276
PdmI GAANNNNTTC 6 cut(s) 2395, 3027, 3472, 4306, 4368, 4459
PflFI GACNNNGTC 1 cut(s) 2374
PfoI TCCNGGA 2 cut(s) 62, 2098
Ppu21I YACGTR 1 cut(s) 2888
PpuMI RGGWCCY 3 cut(s) 527, 1345, 3150
PscI ACATGT 1 cut(s) 2824
PshBI ATTAAT 3 cut(s) 2175, 3612, 3690
Psp5II RGGWCCY 3 cut(s) 527, 1345, 3150
PspEI GGTNACC 2 cut(s) 1672, 3170
PspN4I GGNNCC 8 cut(s) 528, 549, 987, 1683, 2113, 3152, 4126, 4341
PspPI GGNCC 9 cut(s) 66, 224, 527, 985, 1345, 1682, 1962, 3150, 4125
PspPPI RGGWCCY 3 cut(s) 527, 1345, 3150
PspXI VCTCGAGB 1 cut(s) 2681
PstI CTGCAG 2 cut(s) 1915, 2638
PstNI CAGNNNCTG 6 cut(s) 641, 2210, 2405, 2866, 3037, 3604
PsuI RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3980, 4438
PsyI GACNNNGTC 1 cut(s) 2374
PvuII CAGCTG 6 cut(s) 638, 887, 3125, 3247, 3604, 4598
RsaI GTAC 4 cut(s) 495, 2760, 3276, 4498
RsaNI GTAC 4 cut(s) 494, 2759, 3275, 4497
RseI CAYNNNNRTG 6 cut(s) 178, 1443, 2565, 3495, 3528, 3612
SapI GCTCTTC 1 cut(s) 3930
SaqAI TTAA 9 cut(s) 1812, 2061, 2175, 2948, 3195, 3612, 3690, 4551, 4695
Sau96I GGNCC 9 cut(s) 66, 224, 527, 985, 1345, 1682, 1962, 3150, 4125
SduI GDGCHC 6 cut(s) 552, 3016, 3183, 3355, 3397, 4342
SfcI CTRYAG 5 cut(s) 1602, 1911, 2634, 3929, 4083
Sfr274I CTCGAG 1 cut(s) 2681
SfuI TTCGAA 1 cut(s) 767
SinI GGWCC 6 cut(s) 66, 224, 527, 1345, 3150, 4125
SlaI CTCGAG 1 cut(s) 2681
SmiMI CAYNNNNRTG 6 cut(s) 178, 1443, 2565, 3495, 3528, 3612
SmlI CTYRAG 8 cut(s) 999, 1206, 1665, 2681, 2754, 3569, 4167, 4550
SmoI CTYRAG 8 cut(s) 999, 1206, 1665, 2681, 2754, 3569, 4167, 4550
SphI GCATGC 1 cut(s) 3056
SsiI CCGC 9 cut(s) 5, 274, 484, 819, 1443, 1960, 2115, 4120, 4506
SspI AATATT 3 cut(s) 421, 1141, 4369
SspMI CTAG 8 cut(s) 366, 519, 743, 1215, 1859, 2270, 2316, 3753
StyI CCWWGG 7 cut(s) 150, 875, 2918, 3069, 4329, 4578, 4687
TaiI ACGT 4 cut(s) 1548, 2375, 2890, 3430
TatI WGTACW 1 cut(s) 2758
TauI GCSGC 2 cut(s) 486, 1963
Tru1I TTAA 9 cut(s) 1812, 2061, 2175, 2948, 3195, 3612, 3690, 4551, 4695
Tru9I TTAA 9 cut(s) 1812, 2061, 2175, 2948, 3195, 3612, 3690, 4551, 4695
TscAI CASTG 5 cut(s) 185, 607, 3551, 3955, 4516
TseFI GTSAC 6 cut(s) 171, 1201, 1672, 3170, 4286, 4699
Tsp45I GTSAC 6 cut(s) 171, 1201, 1672, 3170, 4286, 4699
TspRI CASTG 5 cut(s) 185, 607, 3551, 3955, 4516
Tth111I GACNNNGTC 1 cut(s) 2374
Vha464I CTTAAG 1 cut(s) 4550
VneI GTGCAC 2 cut(s) 3012, 3351
VpaK11BI GGWCC 6 cut(s) 66, 224, 527, 1345, 3150, 4125
VspI ATTAAT 3 cut(s) 2175, 3612, 3690
XceI RCATGY 3 cut(s) 2828, 2927, 3056
XcmI CCANNNNNNNNNTGG 2 cut(s) 3737, 4595
XhoI CTCGAG 1 cut(s) 2681
XmiI GTMKAC 1 cut(s) 1104
XmnI GAANNNNTTC 6 cut(s) 2395, 3027, 3472, 4306, 4368, 4459
XspI CTAG 8 cut(s) 366, 519, 743, 1215, 1859, 2270, 2316, 3753
Zsp2I ATGCAT 4 cut(s) 1939, 2802, 3054, 4488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.