RchiOBHm_Chr4g0429641

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005720
Physical Location & Seq
Forward (+)
54410679 .. 54417947
7269 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39841

Sequence Viewer

Length: 4815 bp
ATGGCGGAACGCAAGCTCGATCTCCCCGACGATCTCCTCTCCTCCAAACCCTCCGATCAGTCCTGGACCTCCAAAGTTGAAGTTTCTGGAGGAAATGATGAGGAAAAGGTGCTTATGGGGTCATCTGATGAGTTGAAAGATCCAGCAGCCTTGGATAGCAGCATACCTTTGTCACCCCAGTGGCTTTATGCCAAGCCGATTGAGTCTAAGTTGGAAATGCGTGGTCCAACTTCAATTGGAAACTCCACTGACAGCAACCAGAAGGACGGTTGGCGATTGGAAGGATCTGAGGACAAGAAAGATTGGAGGCGACCTGCTACTGAAAGTGAAAGTAGCCGTCGTTGGCGTGAAGAGGAAAGAGAAACTAGCTTGCTTGGTGGTAGAAGAGATCGTAGGAAAACAGAACGCCGTGCTGACAATATTTCATTGAGGGACACAGCTGACAGTAGAGCATTGCCTACAACTGACAGGTGGCATGATGGCCGCAATGAAGTACGGCGTGATAGCAAATGGTCGTCTAGGTGGGGTCCTGAAGACAAAGAAAAGGAGCCCCGAAGTGAGAAGAGAACAGATGTGGAGAAGGATGATGCTCACAGTAACAGTGAGAGTCAATCTCTTGGAGCTAACAACCGTTCAGCTGCTGAGTCAGATTCTCGGGATAAATGGAGGCCACGACATAGAATGGAGGTTCAAACTGGGGGGTCAGCTACTTACCGTGCTGCACCTGGATTTGGACAAGAAAGAGGACGGGTGGAGGGTTCGAATTTGGGATTTACCCTGGGGCGAGGAAGGTCAAGTGGTATTGGGAGATCCGCAGGCACTATTGGCTCTGCTCTTTCAGGAAAGAGTGAAGGTGTCCCTGGGAAACCAAGGCACTCAGCTGATGGTTTTTGCTACCCGAGGGGAAAGCTTCTTGATATTTATCGGCAGCGAAAGCCTGAACTCTCTTTTGATACCATGCCAGATGAGATGGAGGAGGCCCCCTCACTAACTCAAGTTGATTTTGTTGAACCATTGGCTTTTCATGCCCCTGATGCTGATGAGGAGGCCATCCTTAGCGATATATGGAAGGGAAAAATTACAAGTAGTGGGGTAGTATACAATTCATTCAGAAAGGGGAGATCAACTGAAAATATTACAGGTGTGGGAGACTTAGAAGCTGCTGATGGAGTTCTGGGCAATTTGCCCTCGACTGTCACTCAAGAGACTAGCACCTTTCAGGAGGCTGCAAATGCTGATGACTATGGGACTTTGTGGAATTATGGTTCACAGAGGAATGTGATAAATGGAAAAGATGTTGGTCATAAAGAAAATGATAACAGAGCTACTGAAGGAAAGGACCTTGATGGAGTTTCCCTATCAACTCAAAAAAGTAATGGTATCTATGGTGATGTTGAGACTGATGGTTCTTACGATAGTGCAAATCAGCTTCATCTTGGTGGCAATAGGAAAATTGGAGATCCTGCCTTTTCAAATCATCCTGTATCTGATGACATTGAGTTTGCCAATTATTCCGAAATAAGATCCAACCTTCCCGACGTTCCAAATACTCTCTATGGTTTGGCCTCTTCTGAGCAGAATGAGAATAGCAACCCTAGAGTGAAAGAGTTAGAAATGGGTGTTCATCCTGAGGGTTTGTATTACTACTACCTTGATCCTCAAGGGGTGACCCAAGGGCCATATCAGGGGTTAGACATCATTTCATGGTTTGAACAAGGTTTCTTTGGGATAGACTTGCTTGTTCGCTTGGAGGATGCTCCTGAAGGAACACCTTTCAAAGAGTTGGGGGAGTTCATGCCATATCTTAAATCTTGGGATGAGAATGGCAGTATCATAAATCCAAGTTCTAAGCTAGAAGAATCTGGTGGCTTAGGAGGGAGTCTGGAGTCCAGTTTACCCTTTTCTGCAGCAGTTTCAGACAGTAACTATGCATTTCTGGGAAATGACCATCAGCGGCCCATGCCTGAACTAAACAGCCTTTCAGCTCAGCATATTCAGCCAAGAATTTCCGAGCCTGAAGCTCCACTACAACTACATTCAAGGGGTCAAAGCTTTAATGATTTTGTTGAACAAGATGAAGATATTGTGTTTCCTGGAATACATGGAACCGCTGCCTATTCTACTGCACGATCTTCTGGGAGCATACACGATTCTATGGCAAACTCTATTAATCACCTTCCTCCCCCAACTGAAATGACAGAATCTGGTGTGCCTATTCAGAATGATAATAAGTTGCATCCCTTTGGCTTGCTATGGTCTGAGCTAGAAAGCGGCCAATCAAAGCATTCCAAGTCGGCCAATATGCCATCTAGTAAGGGGAGGGCTGTTCCATTTGGTGCAAATCCTGATCCAGCAATTGCTGAGACGTGGTCTGATCTTCATAGAAAAAGTTCAGTCTCTGATCCGAACTTGTATCAGGAAATGTTGACTCCTCGCCAATTATCGCACATGGAACAGGAACCCAGTCATTATGATTTAGCAGAGCAGCTTATGTCGCAGCAAATTCGACAGCAGCAGCAACTCCAACAGCGAAATATGTTGTCTGGTTTTGCACACTTGAATGACAATGTATTAGATCCTTTGCAAAATCAGAATCTTATTCACCATCAACAGTTAGCCAACCATTCTGCAGCAGATCTGGATCACCTTTTGGCACTTCAGAGGCAGGCTCAGCTCGAGCAACATCAACTGCAACAACAGCAGCAGTTTCATCAACAGCAGAAGCTTTTACAGGAGCAACAGCAATCACAAGTACAACAGGTGCTTTTTGAACAATTGTTGCGTGGTCAAATGCATGATCCTACACTTAGGCAGCCACATGTTGATCCTGTAAGAGCAAACAATGTTATTGATCAGGTTCTATTAGAGCAGCAGCTTCTACGTGAACTTCAGCAACGGTCTCACCATCTTCCAAGGCATGTTGATCCAACTATAGAGCAGTTAATTCAAGCAAAGTTTGGTCATACACCTCAAGGACATCAAACAGATTTGTTTGAGCTACTGTCTCGTGCACAGCATGAACAGATTCAGTCGCTGGAACATCAGATGCATGCCAGGCAGTTACCCATGGGAATAAGGCAGCGAATGGAAGAAGAGAGGCATATCGGTTCTGTCTGGCCAGCTGAGGAATCAAATCAGATTTTCAGGACCCATGCTGGTAATCATGGTCACCGAGGGCACTCTTCTGGCTTTAATCCTTTAGATTTTTATCAGAGGCAGCAGAGGCCATCTCATGAGGAGCAGCTGAATCACCTTGACCGGAATCTTTCATTACAGGATCGACTTCAGCAAGGTTTTTATGAGCCTGGCTCACTGCCGTTTGAGCGGTCAATGTCATTACCTGCTGGTGCACCAGGGATGAACTTGGATGTAGTAAATGCTATGGCTCGTGCCCAGGGTTTAGATATGCAGGATTCCATTGGACGTATGCAGTCGGCTGGCCAGTCGGGTCAATTTTCGTCTGGAATCCCTTCCCACAGTGCTCATCATCCTCATGTTCCTAACCAATTTCATGTTTCACATTTGGATGCAATTGAGGGACACTGGCCTGAGAAGAATGATCAGCTTGAGAATGACTGGATGGATGCTCGGTTTCAGCAGCTGCATATTAATGCTGAGAGGCAGAAAAGGGAATCAGAGATCAAGAATACTTCTCAGGATCAAAATTTATGGATGTCAGATGGAATTAATGATGAGAACTCAAAGAGACTACTAATGGATTTACTCCATCAAAAATCTGGCCATCAACCTAGCGAGTCGTTGAATGCGATGAGCAATGGGATGTTCTCTGATAAAAGACTGCCATCTGGCCACTACTCTGGATCAAGCTCCTCAAACCACCTGTTCAATCTCCATGCAGACCAAGAAGCAGGTCTAAATAACTCATTTCGTGTAGGGTCATTTGGTTCTAATCCAGGTGAACTCCTACAGGAAGAGCAGGCCAGCAGTGTGGAAAGCAATGAAAAGTTGATGTATGGATCTAATTCTGGAGCATTGGCTGATAGAGAGTCATTTCTGGCAGGAATGAATGCAACGAGCCAGTCACTTTACACAAATTCTAACATGATAAGTAAGTCTTCTATAGGTAAAGAACTTTCTGAGTTGGAGGGCAGGAAGCGGGGGTCCAAAAGTGAGGGGATAATTATGGGTCGATCTTTTGAGACTCAAGAACGAATGGTTGAACAAGCAGGGTTGTCTGCAACAAATTTTGAGGAGATGTCAAAACACGCTCATAGCATGAATTCTTCCTCCGGTGTTTCAGGTGGGAATGCTGGTTTTTATGGTGACAAGATTGGCGGAAGTAATTCATTTGTCGAACAAACTGGCAAGGATCGGGCTCCCATCCCATCCAAAGGGCAAGAAAATATTCTGCTGAGACGACCTTCTGTCCCAAGTGCTTCAGCATCGCAAGAAGGATTATCAGAACTGATATCAGATCCAGTTCTTAGAGGAAAAAATTCGAGTGCTGCTTCAGATGGGGCAAGGCGAGACGCAGTGGTTAATCCAGTAAACCAAGGTTCTGATGTCATGGCATCTTCGAAAAAAGAAATGCATTTCCGCCGTACATCATCCGCCAGTGATACTGATGTATCAGAGGCATCATTTATCGATATGCTTAAGAGTAATACCAAGAAGATTGCTCCCATGGATGCCCACGCCACAGCTGGATTTGCAGAATCATCAGAAGCAATGCAAGGTGGCCGGAGTGGCAAAAAGAAAGGAAAGAAAGGGAGGCAAATTGATCCTGCCCTCCTTGGTTTTAAAGTCACCAGCAATCGTATAATGATGGGTGAAATTCAACGAATCGACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1604

Amino Acids

178.0

Weight (kDa)

5.51

Isoelectric Point (pI)

55.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 548 - 593 6.8e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 322, 3349, 3860
AccBSI CCGCTC 1 cut(s) 3325
AccI GTMKAC 1 cut(s) 1098
AcoI YGGCCR 8 cut(s) 481, 2272, 2295, 3116, 3439, 3739, 3808, 4699
AcsI RAATTY 9 cut(s) 763, 2004, 2502, 3664, 4054, 4204, 4240, 4456, 4794
AcuI CTGAAG 9 cut(s) 552, 1350, 1782, 2037, 2642, 2873, 3269, 4383, 4455
AfaI GTAC 3 cut(s) 495, 2754, 4564
AfiI CCNNNNNNNGG 3 cut(s) 731, 1685, 4352
AflII CTTAAG 1 cut(s) 4616
AflIII ACRYGT 1 cut(s) 2818
AhdI GACNNNNNGTC 1 cut(s) 4385
AjiI CACGTC 1 cut(s) 2367
AleI CACNNNNGTG 1 cut(s) 178
AloI GAACNNNNNNTCC 2 cut(s) 1249, 1281
Alw21I GWGCWC 3 cut(s) 3013, 3352, 3484
Alw44I GTGCAC 2 cut(s) 3009, 3348
AlwNI CAGNNNCTG 5 cut(s) 641, 2204, 2399, 3034, 3601
Ama87I CYCGRG 3 cut(s) 654, 898, 2675
ApaLI GTGCAC 2 cut(s) 3009, 3348
ApoI RAATTY 9 cut(s) 763, 2004, 2502, 3664, 4054, 4204, 4240, 4456, 4794
ArsI GACNNNNNNTTYG 2 cut(s) 1484, 1516
AseI ATTAAT 3 cut(s) 2169, 3609, 3687
Asp700I GAANNNNTTC 6 cut(s) 2389, 3024, 3469, 4303, 4365, 4456
AspS9I GGNCC 9 cut(s) 66, 224, 527, 979, 1339, 1676, 1956, 3147, 4122
AsuII TTCGAA 2 cut(s) 761, 4538
AvaI CYCGRG 3 cut(s) 654, 898, 2675
AvaII GGWCC 6 cut(s) 66, 224, 527, 1339, 3147, 4122
AxyI CCTNAGG 1 cut(s) 1629
BaeGI GKGCMC 4 cut(s) 3013, 3180, 3352, 3394
BaeI ACNNNNGTAYC 4 cut(s) 4572, 4572, 4605, 4605
BalI TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
BanII GRGCYC 2 cut(s) 552, 4339
BarI GAAGNNNNNNTAC 2 cut(s) 2010, 2042
BauI CACGAG 2 cut(s) 3006, 3387
BbsI GAAGAC 2 cut(s) 540, 4068
Bbv12I GWGCWC 3 cut(s) 3013, 3352, 3484
BbvCI CCTCAGC 1 cut(s) 3123
BceAI ACGGC 5 cut(s) 321, 393, 512, 3301, 4545
BcgI CGANNNNNNTGC 2 cut(s) 2486, 2520
BclI TGATCA 2 cut(s) 2851, 3559
BfaI CTAG 8 cut(s) 366, 519, 1209, 1596, 1853, 2264, 2310, 3750
BfmI CTRYAG 5 cut(s) 1905, 2628, 2931, 3926, 4080
BfrI CTTAAG 1 cut(s) 4616
BfuAI ACCTGC 3 cut(s) 322, 3349, 3860
BglI GCCNNNNNGGC 1 cut(s) 4707
BglII AGATCT 1 cut(s) 2635
BlpI GCTNAGC 2 cut(s) 1986, 2670
Bme18I GGWCC 6 cut(s) 66, 224, 527, 1339, 3147, 4122
BmeRI GACNNNNNGTC 1 cut(s) 4385
BmeT110I CYCGRG 3 cut(s) 654, 898, 2675
BmgBI CACGTC 1 cut(s) 2367
BmgT120I GGNCC 9 cut(s) 66, 224, 527, 979, 1339, 1676, 1956, 3147, 4122
BmiI GGNNCC 8 cut(s) 528, 549, 981, 2107, 2460, 3149, 4123, 4338
BmrI ACTGGG 3 cut(s) 172, 705, 2457
BmuI ACTGGG 3 cut(s) 172, 705, 2457
BpiI GAAGAC 2 cut(s) 540, 4068
BpmI CTGGAG 3 cut(s) 108, 1904, 4008
Bpu10I CCTNAGC 3 cut(s) 1055, 1870, 3123
Bpu1102I GCTNAGC 2 cut(s) 1986, 2670
Bpu14I TTCGAA 2 cut(s) 761, 4538
BpuEI CTTGAG 6 cut(s) 978, 1185, 1644, 2955, 3587, 4149
Bsa29I ATCGAT 1 cut(s) 4608
BsaAI YACGTR 1 cut(s) 2882
BsaBI GATNNNNATC 3 cut(s) 921, 2640, 3207
BsaI GGTCTC 1 cut(s) 2904
BsaWI WCCGGW 2 cut(s) 3258, 4250
Bsc4I CCNNNNNNNGG 3 cut(s) 731, 1685, 4352
Bse21I CCTNAGG 1 cut(s) 1629
Bse3DI GCAATG 5 cut(s) 452, 493, 3781, 3964, 4695
Bse8I GATNNNNATC 3 cut(s) 921, 2640, 3207
BseCI ATCGAT 1 cut(s) 4608
BseJI GATNNNNATC 3 cut(s) 921, 2640, 3207
BseLI CCNNNNNNNGG 3 cut(s) 731, 1685, 4352
BseMI GCAATG 5 cut(s) 452, 493, 3781, 3964, 4695
BseRI GAGGAG 8 cut(s) 26, 31, 989, 1058, 2421, 3251, 3820, 4226
BseSI GKGCMC 4 cut(s) 3013, 3180, 3352, 3394
BsgI GTGCAG 2 cut(s) 705, 2109
BshVI ATCGAT 1 cut(s) 4608
BsiHKAI GWGCWC 3 cut(s) 3013, 3352, 3484
BsiHKCI CYCGRG 3 cut(s) 654, 898, 2675
BsiSI CCGG 3 cut(s) 3259, 4251, 4702
BslFI GGGAC 5 cut(s) 446, 842, 1261, 3552, 4373
BslI CCNNNNNNNGG 3 cut(s) 731, 1685, 4352
BsmBI CGTCTC 3 cut(s) 2357, 4369, 4482
BsmFI GGGAC 5 cut(s) 446, 842, 1261, 3552, 4373
BsmI GAATGC 4 cut(s) 2284, 3769, 4033, 4273
Bso31I GGTCTC 1 cut(s) 2904
BsoBI CYCGRG 3 cut(s) 654, 898, 2675
Bsp119I TTCGAA 2 cut(s) 761, 4538
Bsp1286I GDGCHC 7 cut(s) 552, 3013, 3180, 3352, 3394, 3484, 4339
Bsp1720I GCTNAGC 2 cut(s) 1986, 2670
Bsp19I CCATGG 2 cut(s) 3066, 4644
BspDI ATCGAT 1 cut(s) 4608
BspHI TCATGA 1 cut(s) 3232
BspLI GGNNCC 8 cut(s) 528, 549, 981, 2107, 2460, 3149, 4123, 4338
BspMAI CTGCAG 2 cut(s) 1909, 2632
BspMI ACCTGC 3 cut(s) 322, 3349, 3860
BspQI GCTCTTC 1 cut(s) 3927
BspT104I TTCGAA 2 cut(s) 761, 4538
BspTI CTTAAG 1 cut(s) 4616
BspTNI GGTCTC 1 cut(s) 2904
BsrBI CCGCTC 1 cut(s) 3325
BsrDI GCAATG 5 cut(s) 452, 493, 3781, 3964, 4695
BssNAI GTATAC 1 cut(s) 1099
BssSI CACGAG 2 cut(s) 3006, 3387
BssT1I CCWWGG 8 cut(s) 150, 869, 1672, 2912, 3066, 4513, 4644, 4753
Bst1107I GTATAC 1 cut(s) 1099
Bst2BI CACGAG 2 cut(s) 3006, 3387
Bst6I CTCTTC 7 cut(s) 345, 379, 557, 1573, 3087, 3187, 3927
BstAFI CTTAAG 1 cut(s) 4616
BstBAI YACGTR 1 cut(s) 2882
BstBI TTCGAA 2 cut(s) 761, 4538
BstDSI CCRYGG 2 cut(s) 3066, 4644
BstEII GGTNACC 2 cut(s) 1666, 3167
BstNSI RCATGY 3 cut(s) 2822, 2921, 3053
BstPI GGTNACC 2 cut(s) 1666, 3167
BstSFI CTRYAG 5 cut(s) 1905, 2628, 2931, 3926, 4080
BstSLI GKGCMC 4 cut(s) 3013, 3180, 3352, 3394
BstV2I GAAGAC 2 cut(s) 540, 4068
BstX2I RGATCY 9 cut(s) 139, 284, 809, 1459, 1523, 2575, 2635, 3977, 4435
BstXI CCANNNNNNTGG 2 cut(s) 3818, 3949
BstYI RGATCY 9 cut(s) 139, 284, 809, 1459, 1523, 2575, 2635, 3977, 4435
BstZ17I GTATAC 1 cut(s) 1099
Bsu15I ATCGAT 1 cut(s) 4608
Bsu36I CCTNAGG 1 cut(s) 1629
BsuTUI ATCGAT 1 cut(s) 4608
BtgI CCRYGG 2 cut(s) 3066, 4644
BtgZI GCGATG 2 cut(s) 3782, 4389
BtrI CACGTC 1 cut(s) 2367
BtsI GCAGTG 3 cut(s) 3311, 3952, 4500
BtsIMutI CAGTG 9 cut(s) 185, 246, 607, 3311, 3484, 3541, 3952, 4500, 4582
BveI ACCTGC 3 cut(s) 322, 3349, 3860
CaiI CAGNNNCTG 5 cut(s) 641, 2204, 2399, 3034, 3601
CciI TCATGA 1 cut(s) 3232
Cfr13I GGNCC 9 cut(s) 66, 224, 527, 979, 1339, 1676, 1956, 3147, 4122
ClaI ATCGAT 1 cut(s) 4608
CseI GACGC 1 cut(s) 4499
Csp6I GTAC 3 cut(s) 494, 2753, 4563
CviQI GTAC 3 cut(s) 494, 2753, 4563
DraI TTTAAA 1 cut(s) 4762
DriI GACNNNNNGTC 1 cut(s) 4385
EaeI YGGCCR 8 cut(s) 481, 2272, 2295, 3116, 3439, 3739, 3808, 4699
Eam1104I CTCTTC 7 cut(s) 345, 379, 557, 1573, 3087, 3187, 3927
Eam1105I GACNNNNNGTC 1 cut(s) 4385
EarI CTCTTC 7 cut(s) 345, 379, 557, 1573, 3087, 3187, 3927
EciI GGCGGA 4 cut(s) 20, 4311, 4547, 4561
Eco130I CCWWGG 8 cut(s) 150, 869, 1672, 2912, 3066, 4513, 4644, 4753
Eco24I GRGCYC 2 cut(s) 552, 4339
Eco31I GGTCTC 1 cut(s) 2904
Eco32I GATATC 1 cut(s) 4431
Eco47I GGWCC 6 cut(s) 66, 224, 527, 1339, 3147, 4122
Eco57I CTGAAG 9 cut(s) 552, 1350, 1782, 2037, 2642, 2873, 3269, 4383, 4455
Eco81I CCTNAGG 1 cut(s) 1629
Eco88I CYCGRG 3 cut(s) 654, 898, 2675
Eco91I GGTNACC 2 cut(s) 1666, 3167
EcoO109I RGGNCCY 4 cut(s) 527, 979, 1339, 3147
EcoO65I GGTNACC 2 cut(s) 1666, 3167
EcoRI GAATTC 1 cut(s) 4240
EcoRV GATATC 1 cut(s) 4431
EcoT14I CCWWGG 8 cut(s) 150, 869, 1672, 2912, 3066, 4513, 4644, 4753
EcoT22I ATGCAT 4 cut(s) 1933, 2796, 3051, 4554
EcoT38I GRGCYC 2 cut(s) 552, 4339
ErhI CCWWGG 8 cut(s) 150, 869, 1672, 2912, 3066, 4513, 4644, 4753
Esp3I CGTCTC 3 cut(s) 2357, 4369, 4482
FalI AAGNNNNNCTT 2 cut(s) 4060, 4092
FaqI GGGAC 5 cut(s) 446, 842, 1261, 3552, 4373
FauI CCCGC 1 cut(s) 4110
FbaI TGATCA 2 cut(s) 2851, 3559
FblI GTMKAC 1 cut(s) 1098
FriOI GRGCYC 2 cut(s) 552, 4339
FspBI CTAG 8 cut(s) 366, 519, 1209, 1596, 1853, 2264, 2310, 3750
GsuI CTGGAG 3 cut(s) 108, 1904, 4008
HapII CCGG 3 cut(s) 3259, 4251, 4702
HgaI GACGC 1 cut(s) 4499
HincII GTYRAC 1 cut(s) 2427
HindII GTYRAC 1 cut(s) 2427
HindIII AAGCTT 3 cut(s) 908, 2050, 2723
HpaII CCGG 3 cut(s) 3259, 4251, 4702
Hpy166II GTNNAC 9 cut(s) 1099, 1268, 1895, 2427, 2885, 3011, 3350, 3920, 4510
Hpy8I GTNNAC 9 cut(s) 1099, 1268, 1895, 2427, 2885, 3011, 3350, 3920, 4510
Hpy99I CGWCG 4 cut(s) 32, 342, 1541, 4811
HpyCH4IV ACGT 4 cut(s) 1539, 2366, 2881, 3424
HpySE526I ACGT 4 cut(s) 1539, 2366, 2881, 3424
Ksp22I TGATCA 2 cut(s) 2851, 3559
LguI GCTCTTC 1 cut(s) 3927
MaeI CTAG 8 cut(s) 366, 519, 1209, 1596, 1853, 2264, 2310, 3750
MaeII ACGT 4 cut(s) 1539, 2366, 2881, 3424
MbiI CCGCTC 1 cut(s) 3325
MfeI CAATTG 4 cut(s) 234, 2355, 2774, 3531
MflI RGATCY 9 cut(s) 139, 284, 809, 1459, 1523, 2575, 2635, 3977, 4435
MhlI GDGCHC 7 cut(s) 552, 3013, 3180, 3352, 3394, 3484, 4339
MlsI TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
MluNI TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
MlyI GAGTC 9 cut(s) 212, 616, 653, 1888, 1895, 2422, 3764, 4016, 4156
MmeI TCCRAC 6 cut(s) 192, 251, 1551, 2548, 2951, 4083
Mox20I TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
Mph1103I ATGCAT 4 cut(s) 1933, 2796, 3051, 4554
MroXI GAANNNNTTC 6 cut(s) 2389, 3024, 3469, 4303, 4365, 4456
MscI TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
MslI CAYNNNNRTG 6 cut(s) 178, 1437, 2559, 3492, 3525, 3609
Msp20I TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
MspA1I CMGCKG 9 cut(s) 440, 638, 881, 1954, 2111, 3122, 3244, 3601, 4664
MspCI CTTAAG 1 cut(s) 4616
MspI CCGG 3 cut(s) 3259, 4251, 4702
MunI CAATTG 4 cut(s) 234, 2355, 2774, 3531
Mva1269I GAATGC 4 cut(s) 2284, 3769, 4033, 4273
NcoI CCATGG 2 cut(s) 3066, 4644
NlaIV GGNNCC 8 cut(s) 528, 549, 981, 2107, 2460, 3149, 4123, 4338
NmuCI GTSAC 7 cut(s) 171, 1195, 1666, 3167, 4041, 4283, 4765
NsiI ATGCAT 4 cut(s) 1933, 2796, 3051, 4554
NspI RCATGY 3 cut(s) 2822, 2921, 3053
NspV TTCGAA 2 cut(s) 761, 4538
OliI CACNNNNGTG 1 cut(s) 178
PaeI GCATGC 1 cut(s) 3053
PaeR7I CTCGAG 1 cut(s) 2675
PagI TCATGA 1 cut(s) 3232
PasI CCCWGGG 3 cut(s) 778, 860, 3394
PciI ACATGT 1 cut(s) 2818
PciSI GCTCTTC 1 cut(s) 3927
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 3764
PctI GAATGC 4 cut(s) 2284, 3769, 4033, 4273
PdmI GAANNNNTTC 6 cut(s) 2389, 3024, 3469, 4303, 4365, 4456
PflFI GACNNNGTC 1 cut(s) 2368
PfoI TCCNGGA 2 cut(s) 62, 2092
PleI GAGTC 9 cut(s) 211, 615, 652, 1887, 1894, 2422, 3763, 4015, 4156
PpsI GAGTC 9 cut(s) 211, 615, 652, 1887, 1894, 2422, 3763, 4015, 4156
Ppu21I YACGTR 1 cut(s) 2882
PpuMI RGGWCCY 3 cut(s) 527, 1339, 3147
PscI ACATGT 1 cut(s) 2818
PshBI ATTAAT 3 cut(s) 2169, 3609, 3687
Psp5II RGGWCCY 3 cut(s) 527, 1339, 3147
PspEI GGTNACC 2 cut(s) 1666, 3167
PspN4I GGNNCC 8 cut(s) 528, 549, 981, 2107, 2460, 3149, 4123, 4338
PspPI GGNCC 9 cut(s) 66, 224, 527, 979, 1339, 1676, 1956, 3147, 4122
PspPPI RGGWCCY 3 cut(s) 527, 1339, 3147
PspXI VCTCGAGB 1 cut(s) 2675
PstI CTGCAG 2 cut(s) 1909, 2632
PstNI CAGNNNCTG 5 cut(s) 641, 2204, 2399, 3034, 3601
PsuI RGATCY 9 cut(s) 139, 284, 809, 1459, 1523, 2575, 2635, 3977, 4435
PsyI GACNNNGTC 1 cut(s) 2368
PvuII CAGCTG 7 cut(s) 440, 638, 881, 3122, 3244, 3601, 4664
RsaI GTAC 3 cut(s) 495, 2754, 4564
RsaNI GTAC 3 cut(s) 494, 2753, 4563
RseI CAYNNNNRTG 6 cut(s) 178, 1437, 2559, 3492, 3525, 3609
SapI GCTCTTC 1 cut(s) 3927
Sau96I GGNCC 9 cut(s) 66, 224, 527, 979, 1339, 1676, 1956, 3147, 4122
SchI GAGTC 9 cut(s) 212, 616, 653, 1888, 1895, 2422, 3764, 4016, 4156
SduI GDGCHC 7 cut(s) 552, 3013, 3180, 3352, 3394, 3484, 4339
SfcI CTRYAG 5 cut(s) 1905, 2628, 2931, 3926, 4080
Sfr274I CTCGAG 1 cut(s) 2675
SfuI TTCGAA 2 cut(s) 761, 4538
SinI GGWCC 6 cut(s) 66, 224, 527, 1339, 3147, 4122
SlaI CTCGAG 1 cut(s) 2675
SmiMI CAYNNNNRTG 6 cut(s) 178, 1437, 2559, 3492, 3525, 3609
SmlI CTYRAG 8 cut(s) 993, 1200, 1659, 2675, 2970, 3566, 4164, 4616
SmoI CTYRAG 8 cut(s) 993, 1200, 1659, 2675, 2970, 3566, 4164, 4616
SphI GCATGC 1 cut(s) 3053
SspI AATATT 3 cut(s) 421, 1135, 4366
SspMI CTAG 8 cut(s) 366, 519, 1209, 1596, 1853, 2264, 2310, 3750
StyI CCWWGG 8 cut(s) 150, 869, 1672, 2912, 3066, 4513, 4644, 4753
TaiI ACGT 4 cut(s) 1542, 2369, 2884, 3427
TatI WGTACW 1 cut(s) 2752
TauI GCSGC 3 cut(s) 486, 1957, 2274
TscAI CASTG 9 cut(s) 185, 253, 607, 3318, 3484, 3548, 3952, 4500, 4582
TseFI GTSAC 7 cut(s) 171, 1195, 1666, 3167, 4041, 4283, 4765
Tsp45I GTSAC 7 cut(s) 171, 1195, 1666, 3167, 4041, 4283, 4765
TspRI CASTG 9 cut(s) 185, 253, 607, 3318, 3484, 3548, 3952, 4500, 4582
Tth111I GACNNNGTC 1 cut(s) 2368
Vha464I CTTAAG 1 cut(s) 4616
VneI GTGCAC 2 cut(s) 3009, 3348
VpaK11BI GGWCC 6 cut(s) 66, 224, 527, 1339, 3147, 4122
VspI ATTAAT 3 cut(s) 2169, 3609, 3687
XapI RAATTY 9 cut(s) 763, 2004, 2502, 3664, 4054, 4204, 4240, 4456, 4794
XceI RCATGY 3 cut(s) 2822, 2921, 3053
XcmI CCANNNNNNNNNTGG 2 cut(s) 3734, 4661
XhoI CTCGAG 1 cut(s) 2675
XmiI GTMKAC 1 cut(s) 1098
XmnI GAANNNNTTC 6 cut(s) 2389, 3024, 3469, 4303, 4365, 4456
XspI CTAG 8 cut(s) 366, 519, 1209, 1596, 1853, 2264, 2310, 3750
Zsp2I ATGCAT 4 cut(s) 1933, 2796, 3051, 4554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.