RLG00000007056

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005720
Physical Location & Seq
Reverse (-)
13125522 .. 13132288
6767 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007056

Sequence Viewer

Length: 4827 bp
ATGGCGGAACGCAAGCTCGATCTCCCCGACGATCTCCTCTCCTCCAAACCCTCCGATCAGTCCTCGACCTCCAAAGTTGAAGTGTCTGGAGGAAATGATGAGGAAAAGCTGCTTATGGGGTCATCTGATGAGTTGAAAGATCCAGCAGCCTTGGATAGCAGCATACCTTTGTCACCCCAGTGGCTTTATGCCAAGCCGATTGAGTCTAAGTTGGAAATGCGTGGTCCAACTTCACTCGGAAACTCCACTGACAGCAACCAGAAGGACAGTTGGCGGTTGGAAGGATCTGAGGACAAGAAAGATTGGAGGCGACCTGCTACTGAAAGTGAAAGTAGCCGTCGTTGGCGTGAAGAGGAAAGAGAAACTAGCTTGCTTGGTGGTAGAAGAGATCGTAGGAAAACAGAACGCCGTGCTGACAATATTCCATTGAGGGACACAACTGACAGTAGAGCATTGCCTACAACTGACAGGTGGCATGATGGCCGCAATGAAGTACGGCGTGATAGCAAATGGTCGTCTAGGTGGGGTCCTGAAGACAAAGAAAAGGAGCCCCGAACTGAGAAGAGAACAGATGTGGAGAAGGATGATTCTCACAGTAACAGTGAGAGTCAATCTCTTGGAGCTAACAACCGTTCAGCTGCTGAGCGTGAGTCAGATTCTCGGGATAAATGGAGGCCACGACATAGAATGGAGGTTCAAATTGGGGGGTCAGCTACTTACCGTGCTGCACCTGGATTTGGACTAGAAAGAGGACGGGTGGAGGGTTCGAATTTGGGATTTACCCTGGGGCGAGGAAGGTCAAGTGGTATTGGGAGATCCGCAGGCACTATTGGCTCTGCTCTTTCAGGAAGGAGTGAAGGTGTCCCTGGGAAACCAAGGCACTCAGCTGATGGGTTTTGCTACCCGAGGGGAAAGCTTCTTGATATTTATCGGCAGCGAAAGCCTGAACTATCTTTTGATACCATGCCAGATGAGATGGAGGAGGCCCCTTCACTAACTCAAGTTGATTTTGTTGAACCATTGGCTTTTCATGCCCCTGATGCGGATGAGGAGGCCATCCTTAGCGATATATGGAAGGGAAAAATTACAAGTAGTGGGGTAGTATACAATTCATTCAGAAAGGGGAGATCAACTGAAAATATTACAGGTGTGGGAGACTTAGAAGCTGCTGATGGAGTTCTGGGCAATTTGCCCTCGACTGTCACTCCAGAGACTAGCACCTTTCAGGAGGCTGCAAATGCTGACGACTATGGGACTTTGTGGAATTATGGTTCACAGAGGAATGTGATAAATGGAGAAGATGTTGGTCATAAAGAAAGTGATAACAGAGCTACTGAAGGAAAGGACCTTGATGGAATTTCCCTATCAATTCAAAAAAGTAATGGTATCTATGGTGATGTTGAGACTGATGGTTCTTATGATGGTGCAAATCAGCTTCATGGTGGTGGCAGTAGGAAAATTGGAGATTCTGCCTTTTCAAATCATCCTGTATCTGAGGACATTGAGTTTGCCAATTATTCTGAAATAAGATCCAACCTTACCGACGTTTCAAATACTCTCTATGGTTTGGCCTCTTCTGAGCAGAATGAGAATAGCAACCCTAGAGTGAAAGAGTTAGAAATGGGTGTTCATCCTGAGGGTTTGTATTACTACTACCTTGATCCTCAAGGGGTGACCCAGGGGCCATATCAGGGTTTAGACATAATTTCATGGTTTGAACAAGGTTTCTTTGGGATAGACTTGCTTGTTCGCTTGGAGGATGCTCCTGAAGGAACGCCTTTCAAAGAGTTGGGGGAGTTCATGCCATATCTTAAATCTTGGGGTGAGAATGGCAGTATCATAAATCCAAGTTCGAAGCTAGAAGAATCTGGTGGCTTAGGAGGGAGTCTGGAGTCCAGTTTACCCTTTTCTGCAGCAGTTTCAGACAGTAACTATGCATTCCTGGGAAATGAGCATCAGAGGCCCATGTCTGAACTAAACAGCCTTTCAGCTCAGCATATTCAGCCAAGAATTTCCGAACCTGAAGCTCCACTACAACTACATTCAAGGGGTCAAAGCTTTAATGATTTTGTTGAACAAGATGAAGATATTGTGTTTCCTGGAATACATGGAACCGCTGCCTATTCTACTGCACGATCTTCTGGGAGCATTCACGATTCTATGGCAAACTCTATTAATCACCTTCCTCCCCCAACTGAATTGACAGAATCTGGTGTGCCTATTCAGAATGATAATAAGTTGCATCCCTTTGGCTTGCTATGGTCTGAGCTAGAAAGTGGCCAATCAAAGCATTCCAAGTTGGCCAATATGCCATCTAGTAAGGGGAGGGCTGTTCCATTTGGTGCAAATCCTGATCCAGCAATTGCTGAGACGTGGTCTGATCTTCATAGAAAAAGTTCAGTCTCTGATCCGAACTTGTATCAGGAAATGTTGACTCCTCGCCAATTGTCGCACATGGAACAGGAACCCAGTCATTATGATTTAGCAGAGCAGCTTATGTCGCAGCAAATTCGACAGCAGCAGCAACTCCAACAGCGAAATATGTTGTCTGGTTTTGCACACTTGAATGACAATGTATTAGATCCTTTGCAAAATCAGAATCTTATTCACCATCAACAGTTAGCGAACCATTCTGCAGCAGATCTGGATCACCTTTTGGCACTTCAGAGGCAGGCTCAGCTCGAACAACATCAACTGCAACAACAGCAGCAGTTTCATCAACAGCAGAAGCTTTTGCAGGAGCAACAGCAATCTCAAGTACAACAGGTGCTTTTTGAACAATTGTTGCGTGGTCAAATGCATGATCCTACACTTAGGCAGCCACATGTTGATCCTGTAAGAGCAAACAATGTTATTGATCAGGTTCTATTAGAGCAGCAGCTTCTACGTGAACTTCAGCAACGGTCTCACCATCTTCCAAGGCATGTTGATCCAGCTATGGAGCAGTTAATTCAAGTAGCAAAGTTTGGTCATACACCTCAAGGACATCAAACAGATTTATTTGAGCTACTGTCTCGTGCACAGCATGAACAGATTCAGTCGCTGGAACATCAGATGCATGCCAGGCAGTTACCCATGGGAATGAGGCAGCGAATGGAAGAAGAGAGGCATATCGGTTCTGTCTGGCCAGCTGAGGAATCAAATCAGATTTTCAGGACCCATGCTGGTAATCATGGTCACCGAGGGCACTCTTCTGGCTTTAATCCTTTAGATTTTTATCAGAGGCAGCAGAGGCCATCTCATGAGGATCAGCTGAATCACCTTGACCGGAATCTTTCATTACAGGATCGACTTCAGCAAGGTTTTTATGAGCCTGGCTCGCTGCCGTTTGAGCGGTCAATGTCATTACCTGCTGGTGCACCAGGGATGAACTTGGATGTAGTAAATGCTATGGCTCGTGCCCAGGGTTTAGATATGCAGGATTCCATTGGACGTATGCAGTCGGCTGGCCAGTCGGGTCAATTTTCGTCTGGAATCCCTTCCCACAATGCTCATCATCCTCATGTTCCTAACCAATTTCATGTTTCACATTTGGATGCAATTGAGGGGCACTGGCCTGAGAAGAATGATCAGCTTGAGAATGACTGGATGGACGCTCAGTTTCAGCAGCTGCATATTAATGCTGAGAGGCAGAAAAGGGAATCAGAGATCAAGAATACTTCTCAGGATCAAAATTTGTGGATGTCAGATGGAATCAATGATGAGAACTCAAAGAAACTACTAATGGATTTACTCCATCAAAAATCTGGCCATCAACCTAGCGAGTCGTTGAATGCGATGAGCAATGGGATGTTCTCTGATAAAAGACTGCCATCTGGCCACTACTCTGGATCAAGCTCCACAAACCACCTGTTCAATCTCCATGCAGACCAAGAAGCAGGTCTAAATAACTCATTTCGTGTAGGGTCATTTGGTTCAAATCCAGGTGAACTCCTACAGGAAGAGCAGGCCAGCAGTGTGGAAAGCAATGAAAAGTTGATGTATAGATCTAATTCTGGAGCATTGGCTGATAGAGAGTCATTTCTGGCAGGAATGAATGCAACGAGCCAGTCGCTTTACACAAATTCTAACATGATAAGTAAGTCTTCTATAGGTAAAGAACTTTCTGAGTTGGAGGGCAGGAAGCGGGGGTCCAAAAGTGAGGGGATAATTATGGGTCGATCTTTTGAGACTCAAGAACGAATGGTTGAACAAGCAGGGTTGTCTGCAACAAATTTTGAGGAGAGGTCAAAAAACGCCCATAGCATGAATTCTTCCTCCGGTGTTTCAGGTGGGAGTGCTGGTTTCTATGGTGACAAGATTGGCCGAAGTAATTCATTTGTCGAACAAACTGCCAAGGATCGGGCTCCCATCCCATCTAAAGGGCAAGAAAATATTCTGCTGAGACGGCCTTCTGGCCCAAGTGGTGCTTCAGCATCGCAAGAAGGATTATCAGAACTGATATCAGATCCAGTTCTTAGAGGAAAAAATTTAAGTGCTGCTTCAGATGGGGCAAGGCGAGACGCAGTGGTTAATCCAGTAAACCAAGGTTCTGATGTCATGGCATCTTCGAAAAAAGAAATGCATTTCCGCCGTACATCATCCGCCAGTGATGCTGATGTATCAGAGGCATCATTTATCGATATGCTTAAGAGTAATACCAAGAAGATTGCTCCCATGGATGCCCATGCCACAGCTGGATTTCCAGAATCATCAGAAGCAATGCAAGGTGGCCGGAGTGGCAAAAAGAAAGGAAAGAAAGGGAGGCAAATTGATCCTGCCCTCCTTGGTTTTAAAGTCACCAGCAATCGTATAATGATGGGTGAAATTCAACGAATCGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1609

Amino Acids

178.44

Weight (kDa)

5.52

Isoelectric Point (pI)

55.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 550 - 595 6.8e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 322, 3358, 3869
AccBSI CCGCTC 1 cut(s) 3334
AccI GTMKAC 1 cut(s) 1104
AcoI YGGCCR 9 cut(s) 481, 2278, 2301, 3125, 3448, 3748, 3817, 4303, 4711
AcuI CTGAAG 9 cut(s) 552, 1356, 1788, 2043, 2648, 2879, 3278, 4395, 4467
AfaI GTAC 3 cut(s) 495, 2760, 4576
AfiI CCNNNNNNNGG 5 cut(s) 737, 1042, 1691, 4341, 4361
AflII CTTAAG 1 cut(s) 4628
AflIII ACRYGT 1 cut(s) 2824
AjiI CACGTC 1 cut(s) 2373
AjuI GAANNNNNNNTTGG 2 cut(s) 4393, 4425
AleI CACNNNNGTG 1 cut(s) 178
AloI GAACNNNNNNTCC 2 cut(s) 1255, 1287
Alw21I GWGCWC 2 cut(s) 3022, 3361
Alw44I GTGCAC 2 cut(s) 3018, 3357
AlwNI CAGNNNCTG 5 cut(s) 641, 2210, 2405, 3043, 3610
Ama87I CYCGRG 2 cut(s) 660, 904
ApaLI GTGCAC 2 cut(s) 3018, 3357
ArsI GACNNNNNNTTYG 2 cut(s) 1490, 1522
AseI ATTAAT 2 cut(s) 2175, 3618
Asp700I GAANNNNTTC 5 cut(s) 2395, 3033, 3478, 4312, 4374
AspS9I GGNCC 9 cut(s) 224, 527, 985, 1345, 1682, 1962, 3156, 4131, 4397
AsuII TTCGAA 3 cut(s) 767, 1853, 4550
AvaI CYCGRG 2 cut(s) 660, 904
AvaII GGWCC 5 cut(s) 224, 527, 1345, 3156, 4131
AxyI CCTNAGG 1 cut(s) 1635
BaeGI GKGCMC 5 cut(s) 3022, 3189, 3361, 3403, 3552
BalI TGGCCA 6 cut(s) 2280, 2303, 3127, 3450, 3750, 3819
BanII GRGCYC 2 cut(s) 552, 4348
BarI GAAGNNNNNNTAC 2 cut(s) 2016, 2048
BauI CACGAG 2 cut(s) 3015, 3396
BbsI GAAGAC 2 cut(s) 540, 4077
Bbv12I GWGCWC 2 cut(s) 3022, 3361
BbvCI CCTCAGC 1 cut(s) 3132
BceAI ACGGC 6 cut(s) 321, 393, 512, 3310, 4403, 4557
BcgI CGANNNNNNTGC 4 cut(s) 2492, 2526, 4313, 4347
BclI TGATCA 2 cut(s) 2857, 3568
BfaI CTAG 9 cut(s) 366, 519, 743, 1215, 1602, 1859, 2270, 2316, 3759
BfmI CTRYAG 4 cut(s) 1911, 2634, 3935, 4089
BfrI CTTAAG 1 cut(s) 4628
BfuAI ACCTGC 3 cut(s) 322, 3358, 3869
BglI GCCNNNNNGGC 1 cut(s) 4719
BglII AGATCT 2 cut(s) 2641, 3986
BlpI GCTNAGC 3 cut(s) 642, 1992, 2676
Bme18I GGWCC 5 cut(s) 224, 527, 1345, 3156, 4131
BmeT110I CYCGRG 2 cut(s) 660, 904
BmgBI CACGTC 1 cut(s) 2373
BmgT120I GGNCC 9 cut(s) 224, 527, 985, 1345, 1682, 1962, 3156, 4131, 4397
BmiI GGNNCC 9 cut(s) 528, 549, 987, 1683, 2113, 2466, 3158, 4132, 4347
BmrI ACTGGG 2 cut(s) 172, 2463
BmuI ACTGGG 2 cut(s) 172, 2463
BpiI GAAGAC 2 cut(s) 540, 4077
BplI GAGNNNNNCTC 8 cut(s) 598, 630, 2659, 2691, 3223, 3255, 3302, 3334
BpmI CTGGAG 4 cut(s) 108, 1191, 1910, 4017
Bpu10I CCTNAGC 3 cut(s) 1061, 1876, 3132
Bpu1102I GCTNAGC 3 cut(s) 642, 1992, 2676
Bpu14I TTCGAA 3 cut(s) 767, 1853, 4550
BpuEI CTTGAG 6 cut(s) 984, 1650, 2739, 2964, 3596, 4158
Bsa29I ATCGAT 2 cut(s) 4620, 4818
BsaAI YACGTR 1 cut(s) 2888
BsaBI GATNNNNATC 3 cut(s) 927, 2646, 3216
BsaI GGTCTC 1 cut(s) 2910
BsaWI WCCGGW 2 cut(s) 3267, 4259
BsaXI ACNNNNNCTCC 8 cut(s) 844, 874, 1189, 1219, 1287, 1317, 4267, 4297
Bsc4I CCNNNNNNNGG 5 cut(s) 737, 1042, 1691, 4341, 4361
Bse21I CCTNAGG 1 cut(s) 1635
Bse3DI GCAATG 5 cut(s) 452, 493, 3790, 3973, 4707
Bse8I GATNNNNATC 3 cut(s) 927, 2646, 3216
BseCI ATCGAT 2 cut(s) 4620, 4818
BseJI GATNNNNATC 3 cut(s) 927, 2646, 3216
BseLI CCNNNNNNNGG 5 cut(s) 737, 1042, 1691, 4341, 4361
BseMI GCAATG 5 cut(s) 452, 493, 3790, 3973, 4707
BseRI GAGGAG 6 cut(s) 26, 31, 995, 1064, 2427, 4235
BseSI GKGCMC 5 cut(s) 3022, 3189, 3361, 3403, 3552
BsgI GTGCAG 2 cut(s) 711, 2115
BshVI ATCGAT 2 cut(s) 4620, 4818
BsiHKAI GWGCWC 2 cut(s) 3022, 3361
BsiHKCI CYCGRG 2 cut(s) 660, 904
BsiSI CCGG 3 cut(s) 3268, 4260, 4714
BslFI GGGAC 3 cut(s) 446, 848, 1267
BslI CCNNNNNNNGG 5 cut(s) 737, 1042, 1691, 4341, 4361
BsmBI CGTCTC 3 cut(s) 2363, 4378, 4494
BsmFI GGGAC 3 cut(s) 446, 848, 1267
BsmI GAATGC 5 cut(s) 1937, 2148, 2290, 3778, 4042
Bso31I GGTCTC 1 cut(s) 2910
BsoBI CYCGRG 2 cut(s) 660, 904
Bsp119I TTCGAA 3 cut(s) 767, 1853, 4550
Bsp1286I GDGCHC 7 cut(s) 552, 3022, 3189, 3361, 3403, 3552, 4348
Bsp1720I GCTNAGC 3 cut(s) 642, 1992, 2676
Bsp19I CCATGG 2 cut(s) 3075, 4656
BspDI ATCGAT 2 cut(s) 4620, 4818
BspHI TCATGA 1 cut(s) 3241
BspLI GGNNCC 9 cut(s) 528, 549, 987, 1683, 2113, 2466, 3158, 4132, 4347
BspMAI CTGCAG 2 cut(s) 1915, 2638
BspMI ACCTGC 3 cut(s) 322, 3358, 3869
BspQI GCTCTTC 1 cut(s) 3936
BspT104I TTCGAA 3 cut(s) 767, 1853, 4550
BspTI CTTAAG 1 cut(s) 4628
BspTNI GGTCTC 1 cut(s) 2910
BsrBI CCGCTC 1 cut(s) 3334
BsrDI GCAATG 5 cut(s) 452, 493, 3790, 3973, 4707
BssNAI GTATAC 1 cut(s) 1105
BssSI CACGAG 2 cut(s) 3015, 3396
BssT1I CCWWGG 8 cut(s) 150, 875, 2918, 3075, 4335, 4525, 4656, 4765
Bst1107I GTATAC 1 cut(s) 1105
Bst2BI CACGAG 2 cut(s) 3015, 3396
Bst6I CTCTTC 7 cut(s) 345, 379, 557, 1579, 3096, 3196, 3936
BstAFI CTTAAG 1 cut(s) 4628
BstBAI YACGTR 1 cut(s) 2888
BstBI TTCGAA 3 cut(s) 767, 1853, 4550
BstDSI CCRYGG 2 cut(s) 3075, 4656
BstEII GGTNACC 2 cut(s) 1672, 3176
BstNSI RCATGY 3 cut(s) 2828, 2927, 3062
BstPI GGTNACC 2 cut(s) 1672, 3176
BstSFI CTRYAG 4 cut(s) 1911, 2634, 3935, 4089
BstSLI GKGCMC 5 cut(s) 3022, 3189, 3361, 3403, 3552
BstV2I GAAGAC 2 cut(s) 540, 4077
BstX2I RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3986, 4447
BstXI CCANNNNNNTGG 2 cut(s) 3827, 3958
BstYI RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3986, 4447
BstZ17I GTATAC 1 cut(s) 1105
Bsu15I ATCGAT 2 cut(s) 4620, 4818
Bsu36I CCTNAGG 1 cut(s) 1635
BsuTUI ATCGAT 2 cut(s) 4620, 4818
BtgI CCRYGG 2 cut(s) 3075, 4656
BtgZI GCGATG 2 cut(s) 3791, 4401
BtrI CACGTC 1 cut(s) 2373
BtsI GCAGTG 2 cut(s) 3961, 4512
BtsIMutI CAGTG 7 cut(s) 185, 246, 607, 3550, 3961, 4512, 4594
BveI ACCTGC 3 cut(s) 322, 3358, 3869
CaiI CAGNNNCTG 5 cut(s) 641, 2210, 2405, 3043, 3610
CciI TCATGA 1 cut(s) 3241
Cfr13I GGNCC 9 cut(s) 224, 527, 985, 1345, 1682, 1962, 3156, 4131, 4397
ClaI ATCGAT 2 cut(s) 4620, 4818
CseI GACGC 2 cut(s) 3602, 4511
Csp6I GTAC 3 cut(s) 494, 2759, 4575
CspCI CAANNNNNGTGG 2 cut(s) 3659, 3694
CviQI GTAC 3 cut(s) 494, 2759, 4575
DraI TTTAAA 1 cut(s) 4774
EaeI YGGCCR 9 cut(s) 481, 2278, 2301, 3125, 3448, 3748, 3817, 4303, 4711
Eam1104I CTCTTC 7 cut(s) 345, 379, 557, 1579, 3096, 3196, 3936
EarI CTCTTC 7 cut(s) 345, 379, 557, 1579, 3096, 3196, 3936
EciI GGCGGA 3 cut(s) 20, 4559, 4573
Eco130I CCWWGG 8 cut(s) 150, 875, 2918, 3075, 4335, 4525, 4656, 4765
Eco24I GRGCYC 2 cut(s) 552, 4348
Eco31I GGTCTC 1 cut(s) 2910
Eco32I GATATC 1 cut(s) 4443
Eco47I GGWCC 5 cut(s) 224, 527, 1345, 3156, 4131
Eco57I CTGAAG 9 cut(s) 552, 1356, 1788, 2043, 2648, 2879, 3278, 4395, 4467
Eco81I CCTNAGG 1 cut(s) 1635
Eco88I CYCGRG 2 cut(s) 660, 904
Eco91I GGTNACC 2 cut(s) 1672, 3176
EcoO109I RGGNCCY 4 cut(s) 527, 985, 1345, 3156
EcoO65I GGTNACC 2 cut(s) 1672, 3176
EcoRI GAATTC 1 cut(s) 4249
EcoRV GATATC 1 cut(s) 4443
EcoT14I CCWWGG 8 cut(s) 150, 875, 2918, 3075, 4335, 4525, 4656, 4765
EcoT22I ATGCAT 4 cut(s) 1939, 2802, 3060, 4566
EcoT38I GRGCYC 2 cut(s) 552, 4348
ErhI CCWWGG 8 cut(s) 150, 875, 2918, 3075, 4335, 4525, 4656, 4765
Esp3I CGTCTC 3 cut(s) 2363, 4378, 4494
FalI AAGNNNNNCTT 6 cut(s) 4069, 4101, 4393, 4425, 4465, 4497
FaqI GGGAC 3 cut(s) 446, 848, 1267
FauI CCCGC 1 cut(s) 4119
FbaI TGATCA 2 cut(s) 2857, 3568
FblI GTMKAC 1 cut(s) 1104
FriOI GRGCYC 2 cut(s) 552, 4348
FspBI CTAG 9 cut(s) 366, 519, 743, 1215, 1602, 1859, 2270, 2316, 3759
GsuI CTGGAG 4 cut(s) 108, 1191, 1910, 4017
HapII CCGG 3 cut(s) 3268, 4260, 4714
HgaI GACGC 2 cut(s) 3602, 4511
HincII GTYRAC 1 cut(s) 2433
HindII GTYRAC 1 cut(s) 2433
HindIII AAGCTT 3 cut(s) 914, 2056, 2729
HpaII CCGG 3 cut(s) 3268, 4260, 4714
Hpy166II GTNNAC 9 cut(s) 1105, 1274, 1901, 2433, 2891, 3020, 3359, 3929, 4522
Hpy8I GTNNAC 9 cut(s) 1105, 1274, 1901, 2433, 2891, 3020, 3359, 3929, 4522
Hpy99I CGWCG 3 cut(s) 32, 342, 1547
HpyCH4IV ACGT 4 cut(s) 1545, 2372, 2887, 3433
HpySE526I ACGT 4 cut(s) 1545, 2372, 2887, 3433
Ksp22I TGATCA 2 cut(s) 2857, 3568
LguI GCTCTTC 1 cut(s) 3936
MaeI CTAG 9 cut(s) 366, 519, 743, 1215, 1602, 1859, 2270, 2316, 3759
MaeII ACGT 4 cut(s) 1545, 2372, 2887, 3433
MaeIII GTNAC 9 cut(s) 171, 596, 1201, 1672, 1928, 3069, 3176, 4292, 4777
MbiI CCGCTC 1 cut(s) 3334
MfeI CAATTG 4 cut(s) 2361, 2444, 2780, 3540
MflI RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3986, 4447
MhlI GDGCHC 7 cut(s) 552, 3022, 3189, 3361, 3403, 3552, 4348
MlsI TGGCCA 6 cut(s) 2280, 2303, 3127, 3450, 3750, 3819
MluNI TGGCCA 6 cut(s) 2280, 2303, 3127, 3450, 3750, 3819
MlyI GAGTC 9 cut(s) 212, 616, 659, 1894, 1901, 2428, 3773, 4025, 4165
MmeI TCCRAC 6 cut(s) 192, 251, 258, 1557, 2554, 4092
Mox20I TGGCCA 6 cut(s) 2280, 2303, 3127, 3450, 3750, 3819
Mph1103I ATGCAT 4 cut(s) 1939, 2802, 3060, 4566
MroXI GAANNNNTTC 5 cut(s) 2395, 3033, 3478, 4312, 4374
MscI TGGCCA 6 cut(s) 2280, 2303, 3127, 3450, 3750, 3819
MslI CAYNNNNRTG 7 cut(s) 178, 1443, 2565, 3080, 3501, 3534, 3618
Msp20I TGGCCA 6 cut(s) 2280, 2303, 3127, 3450, 3750, 3819
MspA1I CMGCKG 7 cut(s) 638, 887, 2117, 3131, 3253, 3610, 4676
MspCI CTTAAG 1 cut(s) 4628
MspI CCGG 3 cut(s) 3268, 4260, 4714
MunI CAATTG 4 cut(s) 2361, 2444, 2780, 3540
Mva1269I GAATGC 5 cut(s) 1937, 2148, 2290, 3778, 4042
NcoI CCATGG 2 cut(s) 3075, 4656
NlaIV GGNNCC 9 cut(s) 528, 549, 987, 1683, 2113, 2466, 3158, 4132, 4347
NmuCI GTSAC 6 cut(s) 171, 1201, 1672, 3176, 4292, 4777
NsiI ATGCAT 4 cut(s) 1939, 2802, 3060, 4566
NspI RCATGY 3 cut(s) 2828, 2927, 3062
NspV TTCGAA 3 cut(s) 767, 1853, 4550
OliI CACNNNNGTG 1 cut(s) 178
PaeI GCATGC 1 cut(s) 3062
PagI TCATGA 1 cut(s) 3241
PasI CCCWGGG 4 cut(s) 784, 866, 1678, 3403
PciI ACATGT 1 cut(s) 2824
PciSI GCTCTTC 1 cut(s) 3936
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 3773
PctI GAATGC 5 cut(s) 1937, 2148, 2290, 3778, 4042
PdmI GAANNNNTTC 5 cut(s) 2395, 3033, 3478, 4312, 4374
PflFI GACNNNGTC 1 cut(s) 2374
PfoI TCCNGGA 1 cut(s) 2098
PleI GAGTC 9 cut(s) 211, 615, 658, 1893, 1900, 2428, 3772, 4024, 4165
PpsI GAGTC 9 cut(s) 211, 615, 658, 1893, 1900, 2428, 3772, 4024, 4165
Ppu21I YACGTR 1 cut(s) 2888
PpuMI RGGWCCY 3 cut(s) 527, 1345, 3156
PscI ACATGT 1 cut(s) 2824
PshBI ATTAAT 2 cut(s) 2175, 3618
Psp5II RGGWCCY 3 cut(s) 527, 1345, 3156
PspEI GGTNACC 2 cut(s) 1672, 3176
PspN4I GGNNCC 9 cut(s) 528, 549, 987, 1683, 2113, 2466, 3158, 4132, 4347
PspPI GGNCC 9 cut(s) 224, 527, 985, 1345, 1682, 1962, 3156, 4131, 4397
PspPPI RGGWCCY 3 cut(s) 527, 1345, 3156
PstI CTGCAG 2 cut(s) 1915, 2638
PstNI CAGNNNCTG 5 cut(s) 641, 2210, 2405, 3043, 3610
PsuI RGATCY 8 cut(s) 139, 284, 815, 1529, 2581, 2641, 3986, 4447
PsyI GACNNNGTC 1 cut(s) 2374
PvuII CAGCTG 6 cut(s) 638, 887, 3131, 3253, 3610, 4676
RsaI GTAC 3 cut(s) 495, 2760, 4576
RsaNI GTAC 3 cut(s) 494, 2759, 4575
RseI CAYNNNNRTG 7 cut(s) 178, 1443, 2565, 3080, 3501, 3534, 3618
SapI GCTCTTC 1 cut(s) 3936
Sau96I GGNCC 9 cut(s) 224, 527, 985, 1345, 1682, 1962, 3156, 4131, 4397
SchI GAGTC 9 cut(s) 212, 616, 659, 1894, 1901, 2428, 3773, 4025, 4165
SduI GDGCHC 7 cut(s) 552, 3022, 3189, 3361, 3403, 3552, 4348
SfcI CTRYAG 4 cut(s) 1911, 2634, 3935, 4089
SfuI TTCGAA 3 cut(s) 767, 1853, 4550
SinI GGWCC 5 cut(s) 224, 527, 1345, 3156, 4131
SmiMI CAYNNNNRTG 7 cut(s) 178, 1443, 2565, 3080, 3501, 3534, 3618
SmlI CTYRAG 7 cut(s) 999, 1665, 2754, 2979, 3575, 4173, 4628
SmoI CTYRAG 7 cut(s) 999, 1665, 2754, 2979, 3575, 4173, 4628
SphI GCATGC 1 cut(s) 3062
SspI AATATT 3 cut(s) 421, 1141, 4375
SspMI CTAG 9 cut(s) 366, 519, 743, 1215, 1602, 1859, 2270, 2316, 3759
StyI CCWWGG 8 cut(s) 150, 875, 2918, 3075, 4335, 4525, 4656, 4765
TaiI ACGT 4 cut(s) 1548, 2375, 2890, 3436
TatI WGTACW 1 cut(s) 2758
TauI GCSGC 1 cut(s) 486
TscAI CASTG 7 cut(s) 185, 253, 607, 3557, 3961, 4512, 4594
TseFI GTSAC 6 cut(s) 171, 1201, 1672, 3176, 4292, 4777
Tsp45I GTSAC 6 cut(s) 171, 1201, 1672, 3176, 4292, 4777
TspRI CASTG 7 cut(s) 185, 253, 607, 3557, 3961, 4512, 4594
Tth111I GACNNNGTC 1 cut(s) 2374
Vha464I CTTAAG 1 cut(s) 4628
VneI GTGCAC 2 cut(s) 3018, 3357
VpaK11BI GGWCC 5 cut(s) 224, 527, 1345, 3156, 4131
VspI ATTAAT 2 cut(s) 2175, 3618
XceI RCATGY 3 cut(s) 2828, 2927, 3062
XcmI CCANNNNNNNNNTGG 2 cut(s) 3743, 4673
XmiI GTMKAC 1 cut(s) 1104
XmnI GAANNNNTTC 5 cut(s) 2395, 3033, 3478, 4312, 4374
XspI CTAG 9 cut(s) 366, 519, 743, 1215, 1602, 1859, 2270, 2316, 3759
Zsp2I ATGCAT 4 cut(s) 1939, 2802, 3060, 4566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.