Rh4DG296300

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005720
Physical Location & Seq
Forward (+)
51831278 .. 51838886
7609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG296300.1

Sequence Viewer

Length: 4518 bp
ATGGCAGTTGAAGTTTCTGGAGGAAATGATGAGGAAAAGGTGCTTATGGGGTCATCTGATGAGTTGAAAGATCCAGCAGCCTTGGATAGCAGCATACCTTTGTCACCGCAGTGGCTTTATGCCAAGCCGATTGAGTCTAAGTTGGAAATGCGTGGTCCAACTTCACTCGGAAACTCCACTGACAGCAACCAGAAGGACGGTTGGCGGTTGGAAGGATCTGAGGACAAGAAAGATTGGAGGCGACCTGCTACTGAAAGTGAAAGTAGCCGTCGTTGGCGAGAAGAGGAAAGAGAAACTAGCTTGCTTGGTGGTAGAAGAGATCGTAGGAAAACAGAACGCCGTGCTGAAAATATTCCATTGAGGGACACAACTGACAGTAGAGCATTGCCTACAACTGACAGGTGGCATGATGGCCGCAATGAAGTACGGCGTGATAGCAAATGGTCGTCTAGGTGGGGACCTGAAGACAAAGAAAAGGAGCCCCGAACTGAGAAGAGAACAGATGTGGAGAAGGATGATGCTCACAGTAACAGTGACAGTCAATCTCTTGGAGCTAACAACCGTTCAGCTGCTGAGTCAGATTCTCGGGATAAATGGAGGCCACGACATAGAATGGAGGTTCAAACTGGAGGGTCAGCTACTTACCGTGCTGCACCTGGATTTGGACTAGAAAGAGGACGGGTGGAGGGTTCGAATTTGGGATTTACCCTGGGGCGAGGAAGGTCAAGTGGTATTGGGAGATCTGCAGGCACTATTGGCTCTGCTCTTTCAGGAAAGAGTGAAGGTGTCCCTGGGAAACCAAGGCACTCAGCTGATGGTTTTTGCTACCCGAGGGGAAAGCTTCTTGATATTTATCGGCAGCGAAAGCCTGAACTCTCTTTTGATACCATGCCAGATGAGATGGAGGAGGCCCCCTCACTAACTCAAGTTGATTTTGTTGAACCTTTGGCTTTTCATGCCCCTGATGCTGATGAGGAGGCCATCCTTAGCGATATATGGAAGGGAAAAATTACAAGTAGTGGGGTAGTATACAATTCATTCAGAAAGGGGAGATCAACTGAAAATATTACAGGTGTGGGAGACTTAGAAGCTGCTGATGGAGTGCTGGGCAATTTGCCCTCGACTGTCACTCAAGAGACTAGCACCTTTCAGGAGGCTGCAACTGCTGATGACTATGGGACTTTGTGGAATTATGGTTCACAGAGGAATGTGATAAATGGAAAAGATGTTGGTCATAAAGAAAGTGATAACAGAGCTACTGAAGGAAAGGACCTTGATGGAATTTCCCTATCAACTCAAAAAAGTAATGGTATCTATGGTGATGTTGAGACTGATGGTTCTTATGATAGTGCAAATCAGCTTCATGTTGGTGGCAATGGGAAAATTGGAGATTCTGCCTTTTCAAATCATCCTGTATCTGATGACATTGTGTTTGCCAATTATTCCGAAATAAGATCCAACCTTCCCGACGTTTCAAATACTCTCTATGGTTTGGCCTCTTCTGAGCAGAATGAGAATAGCAACCCTAGAGTGAAAGAGTTAGAAATGGGTGTTCATCCTGAGGGTTTGTCTTACTACTACCTTGATCCTCAAGGGGTGACCCAAGGGCCATATCAGGGGTTAGACATCATTTCATGGTTTGAACAAGGTTTCTTTGGGATAGACTTGCTTGTTCGCTTGGAGGATGCTCCTGAAGGAACACCTTTCAAAGAGTTGGGGGAGTTCATGCCATATCTTAAATCTTGGGATGAGAATGGCAGTATCATAAATCCAAGTTCTAAGCTAGAAGAATCTGGTGGCTTAGGAGGGAGTCTGGAGTCCAGTTTACCCTTTTCTGCAGCAGTTTCAGACAGTAACTATGCATTTCTGGGAAATGACCATCAGCGGCCCATGCCTGAACTAAACAGCCTTTCATCTCAGCATATTCAGCCAAGAATTTCCGAGCCTGAAGCTCCACTACAACTACATTCAAGGGGTCAAAGCTTTAATGATTTTGTTGAACAGGATGAAGATATTGTGTTTCCTGGAATACATGGAACCGCTGCCTATTCTACTGCACGATCTTCTGGGAGCATTCACGATTCTATGGCAAACTCTATTAATCACCTTCCTCCCCCAACTGAAATGACAGAATCTGGTGTGCCTATTCAGAATGATAATAAGTTGCATCCCTTTGGCTTGCTATGGTCTGAGCTAGAAAGCAGCCAATCAAAGCATTCCAAGTCGGCCAATATGCCATCTAGTAAGGGGAGGGCTGTTCCATTTGGTGCAAATCCTGATCCAGCAATTGCTGAGACGTGGTCTGATCTTCATAGAAAAAGTTCAGTCTCTGATCCGAACTTGTATCAGGAAATGTTGACTCCTCGCCAATTGTCGCACATGGAACAGGAACCCAGTCATTATGATTTAGCAGAGCAGCTTATGTCGCAGCAAATTCGACAGCAGCAGCAACTCCAACAGCGAAATATGTTGTCTGGTTTTGCACACTTGAATGACAATGTATTAGATCCTTTGCAAAATCAGAATCTTATTCACCATCAACAGTTAGCCAACCATTCTGCAGCAGATCTGGATCACCTTTTGGCACTTCAGAGGCAGGCTCAGCTCGAGCAACATCAACTGCAACAACAGCAGCAGTTTCATCAACAGCAGAAGCTTTTACAGGAGCAACAGCAATCACAAGTACAACAGGTGCTTTTTGAACAATTGTTGCGTGGTCAAATGCATGATCCTACACTTAGGCAGCCACATGTTGATCCTGTAAGAGCAAACAATGTTATTGATCAGGTTCTATTAGAGCAGCAGCTTCTACGTGAACTTCAGCAACGGTCTCACCATCTTCCAAGGCATGTTGATCCAACTATAGAGCAGTTAATTCAAGCAAAGTTTGGTCATACACCTCAAGGACATCAAACAGATTTGTTTGAGCTACTGTCTCGTGCACAGCATGAACAGATTCAGTCGCTGGAACATCAGATGCATGCCAGGCAGTTACCCATGGGAATAAGGCAGCGAATGGAAGAAGAGAGGCACATTGGTTCTGTCTGGCCAGCTGAGGAATCAAATCAGATTTTCAGGACCCATGCTGGTAATCATGGTCACCGAGGGCACTCTTCTGGCTTTAATCCTTTAGATTTTTATCAGAGGCAGCAGAGGCCATCTCATGAGGAGCAGCTGAATCACCTTGACCGGAATCTTTCATTACAGGATCGACTTCAGCAAGGTTTTTATGAGCCTGGCTCACTGCCGTTTGAGCGGTCAATGTCATTACCTGCTGGTGCACCAGGGATGAACTTGGATGTAGTAAATGCCATGGCTCGTGCCCAGGGTTTAGATATGCAGGATTCCATTGGACGTATGCAGTCGGCTGGCCAGTCGGGTCAATTTTCGTCTGGAATCCCTTCCCATAATGCTCATCATCCTCATGTTCCTAACCAATTTCATGTTTCACATTTGGATGCAATTGAGGGACACTGGCCTGAGAAGAATGATCAGCTTGAGAATGACTGGATGGATGCTCGGTTTCAGCAGCTGCATATTAATGCTGAGAGGCAGAAAAGGGAATCAGAGATCAAGAATACTTCTCAGGATCAAAATTTATGGATGTCAGATGGAATTAATGATGAGAACTCAAAGAGACTACTAATGGATTTACTCCATCAAAAATCTGGCCATCAACCTAGCGAGTCGTTGAATGCGATGAGCAATGGGATGTTCTCTGATAAAAGACTGCCATCTGGCCACTACTCTGGATCAAGCTCCTCAAACCACCTGTTCAATCTCCATGCAGACCAAGAAGCAGGTCTAAATAACTCATTTCGTGTAGGGTCATTTGGTTCTAATCCAGGTGAACTCCTACAGGAAGAGCAGGCCAGCAGTGTGGAAAGCAATGAAAAGTTGATGTATGGATCTAATTCTGGAGCATTGGCTGATAGAGAGTCATTTCTGGCAGGAATGAATGCAACGAGCCAGTCACTTTACACAAATTCTAACATGATAAGTAAGTCTTCTATAGGTAAAGAACTTTCTGAGTTGGAGGGCAGGAAGCGGGGGTCCAAAAGTGAGGGGATAATTATGGGTCAATCTTTTGAGACTCAAGAACGAATGGTTGAACAAGCAGGGTTGTCTGCAACAAATTTTGAGGAGATGTCAAAACACGCCCATAGCATGAATTCTTCCTCCGGTGTTTCAGGTGGGAATGCTGTTTTTTATGGTGACAAGATTGGCGGAAGTAATTCATTTGTCGAACAAACTGCCAAGGATCGGGCTTCCATCCCATCCAAAGGGCAAGAAAATATTCTGCTGAGACGACCTTCTGTCCCAAGTGCTTCAGCATCACAAGAAGGATTATCAGAACTGATATCAGATCCAGTTCTTAGAGGAAAAAATTTGAGTGCTGCTTCAGATGGTATTTTATTTCCAACTCTACTAGTTTCTTATCTTGTATGGTTACATTTTGTTGGAGGGGGAGTGGTGACTGACGTTTCAATCAAATTATCAAATTATACTTGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1505

Amino Acids

167.06

Weight (kDa)

5.38

Isoelectric Point (pI)

55.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 525 - 570 6.3e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 253, 3280, 3791
AccBSI CCGCTC 1 cut(s) 3256
AccI GTMKAC 1 cut(s) 1029
AciI CCGC 8 cut(s) 107, 205, 415, 1885, 2040, 3256, 4048, 4227
AcoI YGGCCR 6 cut(s) 412, 2226, 3047, 3370, 3670, 3739
AcsI RAATTY 9 cut(s) 694, 1281, 1935, 2433, 3595, 3985, 4135, 4171, 4387
AcuI CTGAAG 9 cut(s) 483, 1281, 1713, 1968, 2573, 2804, 3200, 4314, 4386
AfaI GTAC 2 cut(s) 426, 2685
AfiI CCNNNNNNNGG 4 cut(s) 662, 1616, 4263, 4283
AflIII ACRYGT 1 cut(s) 2749
AhdI GACNNNNNGTC 1 cut(s) 4316
AhlI ACTAGT 1 cut(s) 4429
AjiI CACGTC 1 cut(s) 2298
AjnI CCWGG 9 cut(s) 655, 708, 790, 2023, 2984, 3235, 3283, 3324, 3844
AleI CACNNNNGTG 1 cut(s) 109
AloI GAACNNNNNNTCC 2 cut(s) 1180, 1212
Alw21I GWGCWC 2 cut(s) 2944, 3283
Alw44I GTGCAC 2 cut(s) 2940, 3279
AlwNI CAGNNNCTG 5 cut(s) 572, 2135, 2330, 2965, 3532
Ama87I CYCGRG 3 cut(s) 585, 829, 2606
ApaLI GTGCAC 2 cut(s) 2940, 3279
ApoI RAATTY 9 cut(s) 694, 1281, 1935, 2433, 3595, 3985, 4135, 4171, 4387
ArsI GACNNNNNNTTYG 2 cut(s) 1415, 1447
AseI ATTAAT 3 cut(s) 2100, 3540, 3618
Asp700I GAANNNNTTC 6 cut(s) 351, 2320, 2955, 3400, 4234, 4296
AspS9I GGNCC 8 cut(s) 155, 458, 910, 1270, 1607, 1887, 3078, 4053
AsuII TTCGAA 1 cut(s) 692
AvaI CYCGRG 3 cut(s) 585, 829, 2606
AvaII GGWCC 5 cut(s) 155, 458, 1270, 3078, 4053
AxyI CCTNAGG 1 cut(s) 1560
BaeGI GKGCMC 4 cut(s) 2944, 3111, 3283, 3325
BalI TGGCCA 4 cut(s) 3049, 3372, 3672, 3741
BanII GRGCYC 1 cut(s) 483
BarI GAAGNNNNNNTAC 2 cut(s) 1941, 1973
BauI CACGAG 2 cut(s) 2937, 3318
BbsI GAAGAC 2 cut(s) 471, 3999
Bbv12I GWGCWC 2 cut(s) 2944, 3283
BbvCI CCTCAGC 1 cut(s) 3054
BceAI ACGGC 4 cut(s) 252, 324, 443, 3232
BcgI CGANNNNNNTGC 4 cut(s) 2417, 2451, 4235, 4269
BciT130I CCWGG 9 cut(s) 657, 710, 792, 2025, 2986, 3237, 3285, 3326, 3846
BclI TGATCA 2 cut(s) 2782, 3490
BcuI ACTAGT 1 cut(s) 4429
BfmI CTRYAG 6 cut(s) 744, 1836, 2559, 2862, 3857, 4011
BfuAI ACCTGC 3 cut(s) 253, 3280, 3791
BglII AGATCT 2 cut(s) 740, 2566
BlpI GCTNAGC 1 cut(s) 2601
Bme1390I CCNGG 9 cut(s) 657, 710, 792, 2025, 2986, 3237, 3285, 3326, 3846
Bme18I GGWCC 5 cut(s) 155, 458, 1270, 3078, 4053
BmeRI GACNNNNNGTC 1 cut(s) 4316
BmeT110I CYCGRG 3 cut(s) 585, 829, 2606
BmgBI CACGTC 1 cut(s) 2298
BmgT120I GGNCC 8 cut(s) 155, 458, 910, 1270, 1607, 1887, 3078, 4053
BmiI GGNNCC 7 cut(s) 459, 480, 912, 2038, 2391, 3080, 4054
BmrFI CCNGG 9 cut(s) 657, 710, 792, 2025, 2986, 3237, 3285, 3326, 3846
BmrI ACTGGG 1 cut(s) 2388
BmsI GCATC 8 cut(s) 508, 955, 1675, 2176, 2967, 3448, 3505, 4343
BmuI ACTGGG 1 cut(s) 2388
BpiI GAAGAC 2 cut(s) 471, 3999
BplI GAGNNNNNCTC 8 cut(s) 899, 931, 2584, 2616, 3145, 3177, 3224, 3256
BpmI CTGGAG 4 cut(s) 39, 648, 1835, 3939
Bpu10I CCTNAGC 3 cut(s) 986, 1801, 3054
Bpu1102I GCTNAGC 1 cut(s) 2601
Bpu14I TTCGAA 1 cut(s) 692
BpuEI CTTGAG 6 cut(s) 909, 1116, 1575, 2886, 3518, 4080
BsaAI YACGTR 1 cut(s) 2813
BsaBI GATNNNNATC 3 cut(s) 852, 2571, 3138
BsaI GGTCTC 1 cut(s) 2835
BsaWI WCCGGW 2 cut(s) 3189, 4181
BsaXI ACNNNNNCTCC 2 cut(s) 4455, 4485
Bsc4I CCNNNNNNNGG 4 cut(s) 662, 1616, 4263, 4283
Bse1I ACTGG 8 cut(s) 631, 1821, 2394, 3373, 3479, 3512, 3970, 4370
Bse21I CCTNAGG 1 cut(s) 1560
Bse3DI GCAATG 5 cut(s) 383, 424, 1381, 3712, 3895
Bse8I GATNNNNATC 3 cut(s) 852, 2571, 3138
BseBI CCWGG 9 cut(s) 657, 710, 792, 2025, 2986, 3237, 3285, 3326, 3846
BseJI GATNNNNATC 3 cut(s) 852, 2571, 3138
BseLI CCNNNNNNNGG 4 cut(s) 662, 1616, 4263, 4283
BseMI GCAATG 5 cut(s) 383, 424, 1381, 3712, 3895
BseNI ACTGG 8 cut(s) 631, 1821, 2394, 3373, 3479, 3512, 3970, 4370
BseRI GAGGAG 6 cut(s) 920, 989, 2352, 3182, 3751, 4157
BseSI GKGCMC 4 cut(s) 2944, 3111, 3283, 3325
BseYI CCCAGC 1 cut(s) 1105
BsgI GTGCAG 2 cut(s) 636, 2040
BsiHKAI GWGCWC 2 cut(s) 2944, 3283
BsiHKCI CYCGRG 3 cut(s) 585, 829, 2606
BsiSI CCGG 2 cut(s) 3190, 4182
BslFI GGGAC 6 cut(s) 377, 471, 773, 1192, 3483, 4304
BslI CCNNNNNNNGG 4 cut(s) 662, 1616, 4263, 4283
BsmBI CGTCTC 2 cut(s) 2288, 4300
BsmFI GGGAC 6 cut(s) 377, 471, 773, 1192, 3483, 4304
BsmI GAATGC 5 cut(s) 2073, 2215, 3700, 3964, 4204
Bso31I GGTCTC 1 cut(s) 2835
BsoBI CYCGRG 3 cut(s) 585, 829, 2606
Bsp119I TTCGAA 1 cut(s) 692
Bsp1286I GDGCHC 5 cut(s) 483, 2944, 3111, 3283, 3325
Bsp1720I GCTNAGC 1 cut(s) 2601
Bsp19I CCATGG 2 cut(s) 2997, 3312
BspACI CCGC 8 cut(s) 107, 205, 415, 1885, 2040, 3256, 4048, 4227
BspHI TCATGA 1 cut(s) 3163
BspLI GGNNCC 7 cut(s) 459, 480, 912, 2038, 2391, 3080, 4054
BspMAI CTGCAG 3 cut(s) 748, 1840, 2563
BspMI ACCTGC 3 cut(s) 253, 3280, 3791
BspQI GCTCTTC 1 cut(s) 3858
BspT104I TTCGAA 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 2835
BsrBI CCGCTC 1 cut(s) 3256
BsrDI GCAATG 5 cut(s) 383, 424, 1381, 3712, 3895
BsrI ACTGG 8 cut(s) 631, 1821, 2394, 3373, 3479, 3512, 3970, 4370
BssNAI GTATAC 1 cut(s) 1030
BssSI CACGAG 2 cut(s) 2937, 3318
BssT1I CCWWGG 7 cut(s) 81, 800, 1603, 2843, 2997, 3312, 4257
Bst1107I GTATAC 1 cut(s) 1030
Bst2BI CACGAG 2 cut(s) 2937, 3318
Bst2UI CCWGG 9 cut(s) 657, 710, 792, 2025, 2986, 3237, 3285, 3326, 3846
Bst6I CTCTTC 7 cut(s) 276, 310, 488, 1504, 3018, 3118, 3858
BstBAI YACGTR 1 cut(s) 2813
BstBI TTCGAA 1 cut(s) 692
BstC8I GCNNGC 9 cut(s) 302, 748, 2180, 2598, 2982, 3051, 3370, 3870, 3874
BstDSI CCRYGG 2 cut(s) 2997, 3312
BstEII GGTNACC 2 cut(s) 1597, 3098
BstNI CCWGG 9 cut(s) 657, 710, 792, 2025, 2986, 3237, 3285, 3326, 3846
BstNSI RCATGY 3 cut(s) 2753, 2852, 2984
BstPI GGTNACC 2 cut(s) 1597, 3098
BstSCI CCNGG 9 cut(s) 655, 708, 790, 2023, 2984, 3235, 3283, 3324, 3844
BstSFI CTRYAG 6 cut(s) 744, 1836, 2559, 2862, 3857, 4011
BstSLI GKGCMC 4 cut(s) 2944, 3111, 3283, 3325
BstV2I GAAGAC 2 cut(s) 471, 3999
BstX2I RGATCY 8 cut(s) 70, 215, 740, 1454, 2506, 2566, 3908, 4366
BstXI CCANNNNNNTGG 2 cut(s) 3749, 3880
BstYI RGATCY 8 cut(s) 70, 215, 740, 1454, 2506, 2566, 3908, 4366
BstZ17I GTATAC 1 cut(s) 1030
Bsu36I CCTNAGG 1 cut(s) 1560
BtgI CCRYGG 2 cut(s) 2997, 3312
BtgZI GCGATG 1 cut(s) 3713
BtrI CACGTC 1 cut(s) 2298
BtsI GCAGTG 3 cut(s) 116, 3242, 3883
BtsIMutI CAGTG 6 cut(s) 116, 177, 538, 3242, 3472, 3883
BveI ACCTGC 3 cut(s) 253, 3280, 3791
Cac8I GCNNGC 9 cut(s) 302, 748, 2180, 2598, 2982, 3051, 3370, 3870, 3874
CaiI CAGNNNCTG 5 cut(s) 572, 2135, 2330, 2965, 3532
CciI TCATGA 1 cut(s) 3163
Cfr13I GGNCC 8 cut(s) 155, 458, 910, 1270, 1607, 1887, 3078, 4053
Csp6I GTAC 2 cut(s) 425, 2684
CviQI GTAC 2 cut(s) 425, 2684
DriI GACNNNNNGTC 1 cut(s) 4316
EaeI YGGCCR 6 cut(s) 412, 2226, 3047, 3370, 3670, 3739
Eam1104I CTCTTC 7 cut(s) 276, 310, 488, 1504, 3018, 3118, 3858
Eam1105I GACNNNNNGTC 1 cut(s) 4316
EarI CTCTTC 7 cut(s) 276, 310, 488, 1504, 3018, 3118, 3858
EciI GGCGGA 1 cut(s) 4242
Eco130I CCWWGG 7 cut(s) 81, 800, 1603, 2843, 2997, 3312, 4257
Eco24I GRGCYC 1 cut(s) 483
Eco31I GGTCTC 1 cut(s) 2835
Eco32I GATATC 1 cut(s) 4362
Eco47I GGWCC 5 cut(s) 155, 458, 1270, 3078, 4053
Eco57I CTGAAG 9 cut(s) 483, 1281, 1713, 1968, 2573, 2804, 3200, 4314, 4386
Eco81I CCTNAGG 1 cut(s) 1560
Eco88I CYCGRG 3 cut(s) 585, 829, 2606
Eco91I GGTNACC 2 cut(s) 1597, 3098
EcoO109I RGGNCCY 4 cut(s) 458, 910, 1270, 3078
EcoO65I GGTNACC 2 cut(s) 1597, 3098
EcoRI GAATTC 1 cut(s) 4171
EcoRII CCWGG 9 cut(s) 655, 708, 790, 2023, 2984, 3235, 3283, 3324, 3844
EcoRV GATATC 1 cut(s) 4362
EcoT14I CCWWGG 7 cut(s) 81, 800, 1603, 2843, 2997, 3312, 4257
EcoT22I ATGCAT 3 cut(s) 1864, 2727, 2982
EcoT38I GRGCYC 1 cut(s) 483
ErhI CCWWGG 7 cut(s) 81, 800, 1603, 2843, 2997, 3312, 4257
Esp3I CGTCTC 2 cut(s) 2288, 4300
FalI AAGNNNNNCTT 2 cut(s) 3991, 4023
FaqI GGGAC 6 cut(s) 377, 471, 773, 1192, 3483, 4304
FauI CCCGC 1 cut(s) 4041
FbaI TGATCA 2 cut(s) 2782, 3490
FblI GTMKAC 1 cut(s) 1029
FriOI GRGCYC 1 cut(s) 483
GsaI CCCAGC 1 cut(s) 1109
GsuI CTGGAG 4 cut(s) 39, 648, 1835, 3939
HapII CCGG 2 cut(s) 3190, 4182
HincII GTYRAC 1 cut(s) 2358
HindII GTYRAC 1 cut(s) 2358
HindIII AAGCTT 3 cut(s) 839, 1981, 2654
HpaII CCGG 2 cut(s) 3190, 4182
Hpy166II GTNNAC 8 cut(s) 1030, 1199, 1826, 2358, 2816, 2942, 3281, 3851
Hpy8I GTNNAC 8 cut(s) 1030, 1199, 1826, 2358, 2816, 2942, 3281, 3851
Hpy99I CGWCG 2 cut(s) 273, 1472
HpyCH4IV ACGT 5 cut(s) 1470, 2297, 2812, 3355, 4482
HpySE526I ACGT 5 cut(s) 1470, 2297, 2812, 3355, 4482
Ksp22I TGATCA 2 cut(s) 2782, 3490
LguI GCTCTTC 1 cut(s) 3858
LmnI GCTCC 9 cut(s) 478, 551, 1693, 1957, 2070, 2665, 3169, 3764, 3920
LweI GCATC 8 cut(s) 508, 955, 1675, 2176, 2967, 3448, 3505, 4343
MaeII ACGT 5 cut(s) 1470, 2297, 2812, 3355, 4482
MbiI CCGCTC 1 cut(s) 3256
MfeI CAATTG 4 cut(s) 2286, 2369, 2705, 3462
MflI RGATCY 8 cut(s) 70, 215, 740, 1454, 2506, 2566, 3908, 4366
MhlI GDGCHC 5 cut(s) 483, 2944, 3111, 3283, 3325
MlsI TGGCCA 4 cut(s) 3049, 3372, 3672, 3741
MluNI TGGCCA 4 cut(s) 3049, 3372, 3672, 3741
MlyI GAGTC 8 cut(s) 143, 584, 1819, 1826, 2353, 3695, 3947, 4087
MmeI TCCRAC 9 cut(s) 123, 182, 189, 1482, 2479, 2882, 4014, 4441, 4445
Mox20I TGGCCA 4 cut(s) 3049, 3372, 3672, 3741
Mph1103I ATGCAT 3 cut(s) 1864, 2727, 2982
MroXI GAANNNNTTC 6 cut(s) 351, 2320, 2955, 3400, 4234, 4296
MscI TGGCCA 4 cut(s) 3049, 3372, 3672, 3741
MseI TTAA 7 cut(s) 1737, 1986, 2100, 2873, 3123, 3540, 3618
MslI CAYNNNNRTG 6 cut(s) 109, 1368, 2490, 3423, 3456, 3540
Msp20I TGGCCA 4 cut(s) 3049, 3372, 3672, 3741
MspA1I CMGCKG 7 cut(s) 569, 812, 1885, 2042, 3053, 3175, 3532
MspI CCGG 2 cut(s) 3190, 4182
MspR9I CCNGG 9 cut(s) 657, 710, 792, 2025, 2986, 3237, 3285, 3326, 3846
MunI CAATTG 4 cut(s) 2286, 2369, 2705, 3462
Mva1269I GAATGC 5 cut(s) 2073, 2215, 3700, 3964, 4204
MvaI CCWGG 9 cut(s) 657, 710, 792, 2025, 2986, 3237, 3285, 3326, 3846
NcoI CCATGG 2 cut(s) 2997, 3312
NlaIV GGNNCC 7 cut(s) 459, 480, 912, 2038, 2391, 3080, 4054
NmuCI GTSAC 8 cut(s) 102, 533, 1126, 1597, 3098, 3972, 4214, 4474
NsiI ATGCAT 3 cut(s) 1864, 2727, 2982
NspI RCATGY 3 cut(s) 2753, 2852, 2984
NspV TTCGAA 1 cut(s) 692
OliI CACNNNNGTG 1 cut(s) 109
PaeI GCATGC 1 cut(s) 2984
PaeR7I CTCGAG 1 cut(s) 2606
PagI TCATGA 1 cut(s) 3163
PasI CCCWGGG 3 cut(s) 709, 791, 3325
PciI ACATGT 1 cut(s) 2749
PciSI GCTCTTC 1 cut(s) 3858
PcsI WCGNNNNNNNCGW 1 cut(s) 3695
PctI GAATGC 5 cut(s) 2073, 2215, 3700, 3964, 4204
PdmI GAANNNNTTC 6 cut(s) 351, 2320, 2955, 3400, 4234, 4296
PflFI GACNNNGTC 1 cut(s) 2299
PfoI TCCNGGA 1 cut(s) 2023
PleI GAGTC 8 cut(s) 142, 583, 1818, 1825, 2353, 3694, 3946, 4087
PpsI GAGTC 8 cut(s) 142, 583, 1818, 1825, 2353, 3694, 3946, 4087
Ppu21I YACGTR 1 cut(s) 2813
PpuMI RGGWCCY 3 cut(s) 458, 1270, 3078
PscI ACATGT 1 cut(s) 2749
PshBI ATTAAT 3 cut(s) 2100, 3540, 3618
Psp5II RGGWCCY 3 cut(s) 458, 1270, 3078
Psp6I CCWGG 9 cut(s) 655, 708, 790, 2023, 2984, 3235, 3283, 3324, 3844
PspEI GGTNACC 2 cut(s) 1597, 3098
PspFI CCCAGC 1 cut(s) 1105
PspGI CCWGG 9 cut(s) 655, 708, 790, 2023, 2984, 3235, 3283, 3324, 3844
PspN4I GGNNCC 7 cut(s) 459, 480, 912, 2038, 2391, 3080, 4054
PspPI GGNCC 8 cut(s) 155, 458, 910, 1270, 1607, 1887, 3078, 4053
PspPPI RGGWCCY 3 cut(s) 458, 1270, 3078
PspXI VCTCGAGB 1 cut(s) 2606
PstI CTGCAG 3 cut(s) 748, 1840, 2563
PstNI CAGNNNCTG 5 cut(s) 572, 2135, 2330, 2965, 3532
PsuI RGATCY 8 cut(s) 70, 215, 740, 1454, 2506, 2566, 3908, 4366
PsyI GACNNNGTC 1 cut(s) 2299
PvuII CAGCTG 5 cut(s) 569, 812, 3053, 3175, 3532
RsaI GTAC 2 cut(s) 426, 2685
RsaNI GTAC 2 cut(s) 425, 2684
RseI CAYNNNNRTG 6 cut(s) 109, 1368, 2490, 3423, 3456, 3540
SapI GCTCTTC 1 cut(s) 3858
SaqAI TTAA 7 cut(s) 1737, 1986, 2100, 2873, 3123, 3540, 3618
Sau96I GGNCC 8 cut(s) 155, 458, 910, 1270, 1607, 1887, 3078, 4053
SchI GAGTC 8 cut(s) 143, 584, 1819, 1826, 2353, 3695, 3947, 4087
ScrFI CCNGG 9 cut(s) 657, 710, 792, 2025, 2986, 3237, 3285, 3326, 3846
SduI GDGCHC 5 cut(s) 483, 2944, 3111, 3283, 3325
SfaNI GCATC 8 cut(s) 508, 955, 1675, 2176, 2967, 3448, 3505, 4343
SfcI CTRYAG 6 cut(s) 744, 1836, 2559, 2862, 3857, 4011
Sfr274I CTCGAG 1 cut(s) 2606
SfuI TTCGAA 1 cut(s) 692
SinI GGWCC 5 cut(s) 155, 458, 1270, 3078, 4053
SlaI CTCGAG 1 cut(s) 2606
SmiMI CAYNNNNRTG 6 cut(s) 109, 1368, 2490, 3423, 3456, 3540
SmlI CTYRAG 7 cut(s) 924, 1131, 1590, 2606, 2901, 3497, 4095
SmoI CTYRAG 7 cut(s) 924, 1131, 1590, 2606, 2901, 3497, 4095
SpeI ACTAGT 1 cut(s) 4429
SphI GCATGC 1 cut(s) 2984
SsiI CCGC 8 cut(s) 107, 205, 415, 1885, 2040, 3256, 4048, 4227
SspI AATATT 3 cut(s) 352, 1066, 4297
StyD4I CCNGG 9 cut(s) 655, 708, 790, 2023, 2984, 3235, 3283, 3324, 3844
StyI CCWWGG 7 cut(s) 81, 800, 1603, 2843, 2997, 3312, 4257
TaiI ACGT 5 cut(s) 1473, 2300, 2815, 3358, 4485
TaqI TCGA 6 cut(s) 692, 1121, 2437, 2607, 3211, 4245
TatI WGTACW 1 cut(s) 2683
TauI GCSGC 2 cut(s) 417, 1888
Tru1I TTAA 7 cut(s) 1737, 1986, 2100, 2873, 3123, 3540, 3618
Tru9I TTAA 7 cut(s) 1737, 1986, 2100, 2873, 3123, 3540, 3618
TscAI CASTG 6 cut(s) 116, 184, 538, 3249, 3479, 3883
TseFI GTSAC 8 cut(s) 102, 533, 1126, 1597, 3098, 3972, 4214, 4474
Tsp45I GTSAC 8 cut(s) 102, 533, 1126, 1597, 3098, 3972, 4214, 4474
TspRI CASTG 6 cut(s) 116, 184, 538, 3249, 3479, 3883
Tth111I GACNNNGTC 1 cut(s) 2299
VneI GTGCAC 2 cut(s) 2940, 3279
VpaK11BI GGWCC 5 cut(s) 155, 458, 1270, 3078, 4053
VspI ATTAAT 3 cut(s) 2100, 3540, 3618
XapI RAATTY 9 cut(s) 694, 1281, 1935, 2433, 3595, 3985, 4135, 4171, 4387
XceI RCATGY 3 cut(s) 2753, 2852, 2984
XcmI CCANNNNNNNNNTGG 1 cut(s) 3665
XhoI CTCGAG 1 cut(s) 2606
XmiI GTMKAC 1 cut(s) 1029
XmnI GAANNNNTTC 6 cut(s) 351, 2320, 2955, 3400, 4234, 4296
Zsp2I ATGCAT 3 cut(s) 1864, 2727, 2982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.