MD16G1124000.v1.1

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005720
Physical Location & Seq
Reverse (-)
8952929 .. 8960238
7310 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1124000.v1.1.491

Sequence Viewer

Length: 4710 bp
ATGGCGGAAGGCAAGCTCGATCTGCCCGACGATCTCCTCTCCTCCAAACCCTCCGATCAGTCCTGGACCTCCAAAGTTGAAGCCTCTGGAGGAAATGATGAAGAAAAGGTGCTTATTGGGTCATCTGATGACTCGAAAGATCCAGCTGCCTCAGAGAGCATACCTTTGTCCCCCCAGTGGCTTTATGCCAAACCCAGTGAGTCTAAGTTGGAAATGCGCGGCCCAAGTTCCCTTGGAAACTCTACTGATAGCAACCAGAAGGAAGGTTGGCGTTTGGAAGGATCTGATGACAAAAAAGATTGGAGGCGGCCTGCCACTGAAAGTGAAAGTAGCCGTCGTTGGCGTGAAGAGGAAAGAGAAACTAGCTTGCTTGGTGGCAGAAGAGACCGAAGGAAACCAGAACGCCGTGTCGACAGTGTTCCAGTGAGAGACACAACTGACAATAGGGCACTGCCTGCATCTGAAAGGTGGAATGATGGCCGTACTTCTGCACATGAAGCACGGCGTGATAGCAAATGGTCATCAAGGTGGGGTCCTGAAGACAAAGAAAAGGAGACTCGAACTGAAAAGAAAACAGATGTGGAGAAGGAATATGCTCACAGTAACAGTGACAATCAATCCCTTGGAGGTAACAACCGTTCAGCTTCTGAGCGCGAGTCAGATTCTCGGGATAAATGGAGGCCACGCCATAGGGATCTTCACTCTGGTGGGTCAAATGCTTACCGTGCTGCACCAGGATTTGGACTTGAAAAAGGACGGGTGGAGGGTTCAAATTTGGGATTTACCCTTGGAAGAGGGAGGGCATCTGGTGTCGGGAGATCATCTGCAGGTGTTATTGGCTCTGCTCCTTCTGGAAAAAGTGAAAGTGTTCCTGGGAAACCTAGGCACTCAGCTGATAGTTTTTGCTACCCAAGGGGAAAGCTTCTTGACATTTATCGCCAGCGAAAGCTTGATCTATCTTTTGCTACCATGCCAGATGAGATGGAAGAATCGCCTCCTACAACTCAAGTGGGCTTTGTGGAGCCATTAGCTTTTCTTGCCCCCGATGGTGAAGAGGAGGCCATCCTCAGTGATATATGGAACGGAAAGATTACAAGTAGTGGGGTAGTATACAATTCATTTAGAAAGGGAAGATCAACTGAAAACAATACAGGTGGCGGAGACTTGGAAGCTGTTGATGGAGTTCTGGGTATTTTGCCCTCGAGCATCACAGAAGAGACAAACAATATCTTGCAGGAGTCTGCAAATGCTGATGATTATGGGACTTCATGGAATTCTGGTGCACAAAGGAATGTGGTAGATGAAAAAGATGTCAATCATAAAGAATGGGAAACCAAATCTAGTGCAGAAAAGGACCTTGATGGATTTTCTCAAACATTTCAGAAAAGTAATGGTATCTGCAGTGATGTTGAGACTGATGGTGGCAGTAGGAAAAATGAAGCTTCTTTTTTATCCAGCCATTCTTTGTCGAATGACATTGAGTTTGCAACTTCTGACTTAAGATCAAAACTTCACGATGGTTCAAATACTCTCTATGCTTTTGGCTCGTCTGAGCAGAATCTGAATAATAATTCTCGGGTGAAAGAGTTAGAAAGGGATGTCCCTCCTGAGGATTTATGTTTGTATTACCTTGATCCTCAAGGGGCGGTTCAGGGACCATATTTGGGGGTAGACATTATTTCATGGTTTGAACAAGGGTTTTTTGGGACAGACTTGCCTGTTCGATTGGCAGATACTCCTGAAGGAACACCTTTCAAAGAATTGGGGGAGGTCATTCCGCATCTGAAAGTTTGGGATGCGCATGGGAATATTGTAAATCCAACTTCTCATATAGAAGAATCAAGTGGCTCCTTAGGGAATATAGAGTCTAGCTTACCTTCTTCCGATCTAAATAGCCTTTCAACTCTGCATATTCAACCTAGAAATTCTGAGCCTGAAGCTCCACTACAACTGCCACATTCAACGGGTCAAAGCTTTAATGATTTTTGTGCAGAAGATGAAGATATTGTGTTTCCAGGAATACCTGGAACCACCGGCTATTCTACTGCAAGATCTTCTGGAGCCATTCATGATTCTATTGAAAATTCTATTGGCCACCTTCCTTCTCCAACTGAGCTGACAGAATCTGGTGTGCCTATTCAGAATGATAATAAGTTGCACCCCTTTGGCTTGCTTTGGTCTGAGTTAGAAGGCTGTCAAACAAAACAAGTCAAGCCAGCCAATAGGCCTTCTAACATGGGGAGGGCTGTTCCATTTAGTGGAATTTCTGATACAGCGATTCTTGCTGAGACATGGTCTGATGTTCATAGAAAAAATGCAGTCTCTGATACAAACCTGTATCAAGACATGGTAACCCCACGTCAATTGGCACACATGGAACAGGAACCCAGTCATTATGATTTAGCGGAGCAGCTTATGTCACAGCAAATTCGACAGCAGCAGCTCCAACAGCGGAATATGTTGTCTAGCTTTGGACACTTGAATGATGCAGCATTAGAGCATTTGCCAAGTCAGAATCTTATCCACCAACAGTTAGCCAACCATTCTTCAGCAGATCTGGATCATCTTTTGGCACTTAAGATGCAACAGCACCGGGAGGCTCAGCTCCAGCAACATCAGCAACTGCAACAGCAGCAGTTTCATCACCAGCAGAAGCTTTTACAGGAGCAACAGCAGTCCCAACTGCAACAGGTGCTTCTTGAACAACTGCTGCGTGGTCAAATGCATGATCCTGCGCTTAGGCAGCCACACATTGATCCTGTCAGAGCAAACAACGTTATTGAACAGGTTCTATTAGAGCAGCGCCTTATGCATGAACTCCAACAAAGGTCTCACCATTTTCCAAGGCATGTTGATCCATCTTTGGAGCAGCTTGTCCAAGCGAAGTTTGGTCAGTCACCCCCACAAGCAGATTTTCTTGATCTACTGTCTCGTGCGCAGCATGAACAAATTCAATCATTGGAACATCAGATGCAAGCCAGGCAGTTACCAACGGGGATGAGACAGAGGATGGAAGACGACAGACATGGTGGTTCTGTCTGGCCAGCAGATGAGTCAAATCAGTTTTTGAGGACCCATGCTGGTGCTCAGCGAGCTCACTCTTCCGGATTTAGTCCTTTAGAATTTTATCAGAAGCAACAGAGGCCATCCCATGAGGAGCAGCTTAGCCAACTTGACCGTAATCTTTCATTGCAGGATCGACTTCAGCAAGGGTTTTATGAGCCTGGCTCTCATCCGTTTGAGCGGTCTATGTCACTGCCTGCTGGGGCACAAGGGATGAACTTGGATGCTGTAAATGCCATGGCTCGTGCTCAGGGCTTGGATATGCAGGATTCCCTTGGACGGATGCCATCGGGTGGTCAATTGGGAACATTTTCATCTGGAATCCATTCTCATAATCCTCATCACCCTCTTCCTAACCAATTTCATGCTTCACATTTGGATGCAATTGAGGGTCACTGGCCTGAGAAGAATGAACAGCTAGAAAATGATTGGATGGACTCTCGGTTTCAGCAGTTGCATATTAATGCTGAGCGGCAGAAAAGGGAGTCAGAGATCAATATTCCTTCTCAGGATCGAGGTTTATGGATGTCAGATGGATTGAATGAAGAGCATTCAAAGAGACTATTGATGGAGTTACTCCATAAAAAATCAGCCCATCAACCTGCTGAGTCATTGAATGTCAACAATGGCATCTTCTCTGATAAAAGGCCTTCATCTGGCATGTACTCTGGTTCAAGCTCTTCAAGTCACCCATTCAGTCTTCATTCAGACCAAGAAGCAGGTCTAAATAATTCATTTCGAGTAGGATCATATGGTTCAAATCATATGGAGCTCCCACAGGAAGAGCGGGCCAGCAGCATGGAAAGCAATGAAAAGTTGGTGTATAGATCTGATTCTGGAGCATTGGTTGAGAGAGAGTCATTCTTGGCTGGCATCAATGCTACGAGTCAGCCGATTTACACAAATTCTAACATCATTAGTAAGTCTTCTATCAGTAAAGAGGTTTCGGAGTTGGAGGGAAGGAAGCGTGGGACGAAGAGTGAGGGGATCATTAGGGGACAAGCGTTTGACACTCGAGAACAAATGGTTGAACAAGCAGTGTTGGCTGCTCCAGATTATGAGGAGAGGTCAGCAAATTCCCTTAACATGCGTTCGTCATCTGGTGTTTCAGGCTTTCATAGCGACAAGATTGGCCGAAGTAATTCATTTGCGGAAGAAACAACCAGGGATCGGGTTCCAGCCCCATCCAAAACTCAAGATAATATTCTGCTGAGACGACCTCCTGTCTCAAGCGCTTCAGCATCCCACGAAGGATTGTCAGAACTGATCTCTAATCCAGTCTTCCGAGGACAAAGTGCAAGTGCTGCTCCAGATGGGGGGAGGCAAGACCCCGTTGTTGTTCATCCAGTAAACCAAGGTTCTGATGCCTTGCCGTCATCTTCAAAGAAAGAAATTCAATTTCGCCGTACTTCTTCCGCCAGCGATGCTGATGTGTCAGAGGCTTCATTTATGGATATGCTTAAGAGTAATACCAAGAAGCTGGCTCCGACGGATGCCCATGCGACGGCGGGAGAAGATGCAATGCAAGGGAACCGGAGTGGCAAAAAGAAGGGAAAGAAAGGGAGGCAAATCGATCCTGCCCTCCTCGGTTTTAAAGTTACCAGCAACCGGATAATGATGGGCGAAATCCATCGCATAGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1570

Amino Acids

173.98

Weight (kDa)

5.55

Isoelectric Point (pI)

59.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 542 - 586 6.1e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 818
Acc16I TGCGCA 2 cut(s) 1800, 2937
Acc36I ACCTGC 3 cut(s) 818, 3673, 3773
AccB7I CCANNNNNTGG 3 cut(s) 740, 2258, 3356
AccBSI CCGCTC 3 cut(s) 3244, 3535, 3850
AccI GTMKAC 3 cut(s) 411, 1110, 1671
AccII CGCG 2 cut(s) 219, 654
AccIII TCCGGA 1 cut(s) 3104
AclI AACGTT 1 cut(s) 2775
AcoI YGGCCR 4 cut(s) 478, 2092, 3041, 4195
AcuI CTGAAG 6 cut(s) 558, 1761, 1956, 2532, 3188, 4284
AdeI CACNNNGTG 1 cut(s) 506
AfaI GTAC 3 cut(s) 484, 3728, 4471
AfeI AGCGCT 1 cut(s) 4297
AflII CTTAAG 3 cut(s) 1498, 2576, 4523
AhdI GACNNNNNGTC 1 cut(s) 4286
AjiI CACGTC 1 cut(s) 2360
AjnI CCWGG 8 cut(s) 62, 733, 871, 2014, 2023, 2978, 3223, 4226
AjuI GAANNNNNNNTTGG 2 cut(s) 2073, 2105
AleI CACNNNNGTG 1 cut(s) 705
Alw21I GWGCWC 5 cut(s) 1285, 3088, 3097, 3313, 3837
Alw44I GTGCAC 1 cut(s) 1281
AlwNI CAGNNNCTG 5 cut(s) 647, 1561, 2126, 2324, 2623
Ama87I CYCGRG 4 cut(s) 666, 1201, 1575, 4077
Aor13HI TCCGGA 1 cut(s) 3104
Aor51HI AGCGCT 1 cut(s) 4297
ApaLI GTGCAC 1 cut(s) 1281
ArsI GACNNNNNNTTYG 2 cut(s) 1466, 1498
AseI ATTAAT 1 cut(s) 3525
Asp700I GAANNNNTTC 6 cut(s) 867, 2787, 2949, 3373, 3388, 4204
AspA2I CCTAGG 1 cut(s) 881
AspLEI GCGC 7 cut(s) 219, 654, 1801, 2737, 2805, 2938, 4298
AspS9I GGNCC 7 cut(s) 66, 221, 533, 1354, 1655, 3072, 3852
AsuC2I CCSGG 1 cut(s) 2594
AsuHPI GGTGA 7 cut(s) 1061, 1591, 2636, 2825, 2889, 3398, 3743
AvaI CYCGRG 4 cut(s) 666, 1201, 1575, 4077
AvaII GGWCC 5 cut(s) 66, 533, 1354, 1655, 3072
AvrII CCTAGG 1 cut(s) 881
AxyI CCTNAGG 2 cut(s) 1608, 1852
BaeGI GKGCMC 3 cut(s) 451, 1285, 3271
BalI TGGCCA 2 cut(s) 2094, 3043
BanII GRGCYC 2 cut(s) 3097, 3837
BarI GAAGNNNNNNTAC 2 cut(s) 1929, 1961
BauI CACGAG 2 cut(s) 2931, 3306
BbsI GAAGAC 5 cut(s) 546, 3021, 3755, 3981, 4336
Bbv12I GWGCWC 5 cut(s) 1285, 3088, 3097, 3313, 3837
BceAI ACGGC 7 cut(s) 318, 390, 465, 518, 4420, 4452, 4584
BciT130I CCWGG 8 cut(s) 64, 735, 873, 2016, 2025, 2980, 3225, 4228
BcnI CCSGG 1 cut(s) 2594
BfaI CTAG 7 cut(s) 363, 882, 1341, 1869, 1920, 2466, 3482
BfmI CTRYAG 2 cut(s) 825, 1399
BfoI RGCGCY 2 cut(s) 2806, 4299
BfrI CTTAAG 3 cut(s) 1498, 2576, 4523
BfuAI ACCTGC 3 cut(s) 818, 3673, 3773
BglII AGATCT 3 cut(s) 2051, 2554, 3890
BlnI CCTAGG 1 cut(s) 881
BlpI GCTNAGC 4 cut(s) 2601, 3087, 3164, 3531
Bme1390I CCNGG 9 cut(s) 64, 735, 873, 2016, 2025, 2594, 2980, 3225, 4228
Bme18I GGWCC 5 cut(s) 66, 533, 1354, 1655, 3072
BmeRI GACNNNNNGTC 1 cut(s) 4286
BmeT110I CYCGRG 4 cut(s) 666, 1201, 1575, 4077
BmgBI CACGTC 1 cut(s) 2360
BmgT120I GGNCC 7 cut(s) 66, 221, 533, 1354, 1655, 3072, 3852
BmrFI CCNGG 9 cut(s) 64, 735, 873, 2016, 2025, 2594, 2980, 3225, 4228
BmrI ACTGGG 3 cut(s) 169, 189, 2382
BmuI ACTGGG 3 cut(s) 169, 189, 2382
BpiI GAAGAC 5 cut(s) 546, 3021, 3755, 3981, 4336
BplI GAGNNNNNCTC 4 cut(s) 3212, 3244, 4267, 4299
BpmI CTGGAG 6 cut(s) 108, 2079, 2591, 3921, 4098, 4356
Bpu10I CCTNAGC 2 cut(s) 2738, 3312
Bpu1102I GCTNAGC 4 cut(s) 2601, 3087, 3164, 3531
BpuEI CTTGAG 4 cut(s) 990, 1623, 4242, 4276
BpuMI CCSGG 1 cut(s) 2594
Bsa29I ATCGAT 1 cut(s) 4635
BsaBI GATNNNNATC 2 cut(s) 1314, 2559
BsaI GGTCTC 2 cut(s) 378, 2835
BsaWI WCCGGW 3 cut(s) 3104, 4596, 4671
BsaXI ACNNNNNCTCC 2 cut(s) 3894, 3924
Bse118I RCCGGY 1 cut(s) 2033
Bse1I ACTGG 7 cut(s) 175, 195, 422, 2388, 3464, 4340, 4409
Bse21I CCTNAGG 2 cut(s) 1608, 1852
Bse3DI GCAATG 3 cut(s) 3188, 3877, 4590
Bse8I GATNNNNATC 2 cut(s) 1314, 2559
BseAI TCCGGA 1 cut(s) 3104
BseBI CCWGG 8 cut(s) 64, 735, 873, 2016, 2025, 2980, 3225, 4228
BseCI ATCGAT 1 cut(s) 4635
BseJI GATNNNNATC 2 cut(s) 1314, 2559
BseMI GCAATG 3 cut(s) 3188, 3877, 4590
BseNI ACTGG 7 cut(s) 175, 195, 422, 2388, 3464, 4340, 4409
BseRI GAGGAG 6 cut(s) 26, 31, 1070, 3170, 4139, 4637
BseSI GKGCMC 3 cut(s) 451, 1285, 3271
BseYI CCCAGC 1 cut(s) 3263
BsgI GTGCAG 4 cut(s) 474, 714, 1365, 2010
Bsh1236I CGCG 2 cut(s) 219, 654
BshVI ATCGAT 1 cut(s) 4635
BsiHKAI GWGCWC 5 cut(s) 1285, 3088, 3097, 3313, 3837
BsiHKCI CYCGRG 4 cut(s) 666, 1201, 1575, 4077
BsiSI CCGG 5 cut(s) 2034, 2593, 3105, 4597, 4672
BslFI GGGAC 8 cut(s) 154, 1276, 1586, 1668, 1720, 2662, 4048, 4074
BsmBI CGTCTC 1 cut(s) 4270
BsmFI GGGAC 8 cut(s) 154, 1276, 1586, 1668, 1720, 2662, 4048, 4074
BsmI GAATGC 1 cut(s) 3613
Bso31I GGTCTC 2 cut(s) 378, 2835
BsoBI CYCGRG 4 cut(s) 666, 1201, 1575, 4077
Bsp1286I GDGCHC 7 cut(s) 451, 1285, 3088, 3097, 3271, 3313, 3837
Bsp13I TCCGGA 1 cut(s) 3104
Bsp1720I GCTNAGC 4 cut(s) 2601, 3087, 3164, 3531
Bsp19I CCATGG 1 cut(s) 3300
BspDI ATCGAT 1 cut(s) 4635
BspEI TCCGGA 1 cut(s) 3104
BspFNI CGCG 2 cut(s) 219, 654
BspHI TCATGA 1 cut(s) 2068
BspMAI CTGCAG 2 cut(s) 829, 1403
BspMI ACCTGC 3 cut(s) 818, 3673, 3773
BspQI GCTCTTC 3 cut(s) 3603, 3748, 3840
BspTI CTTAAG 3 cut(s) 1498, 2576, 4523
BspTNI GGTCTC 2 cut(s) 378, 2835
BsrBI CCGCTC 3 cut(s) 3244, 3535, 3850
BsrDI GCAATG 3 cut(s) 3188, 3877, 4590
BsrFI RCCGGY 1 cut(s) 2033
BsrI ACTGG 7 cut(s) 175, 195, 422, 2388, 3464, 4340, 4409
BssAI RCCGGY 1 cut(s) 2033
BssNAI GTATAC 1 cut(s) 1111
BssSI CACGAG 2 cut(s) 2931, 3306
BssT1I CCWWGG 9 cut(s) 232, 622, 787, 881, 911, 2843, 3300, 3337, 4417
Bst1107I GTATAC 1 cut(s) 1111
Bst2BI CACGAG 2 cut(s) 2931, 3306
Bst2UI CCWGG 8 cut(s) 64, 735, 873, 2016, 2025, 2980, 3225, 4228
Bst4CI ACNGT 8 cut(s) 416, 602, 608, 638, 725, 2532, 2928, 3179
BstAFI CTTAAG 3 cut(s) 1498, 2576, 4523
BstAPI GCANNNNNTGC 3 cut(s) 455, 2692, 4367
BstDSI CCRYGG 1 cut(s) 3300
BstEII GGTNACC 1 cut(s) 2350
BstFNI CGCG 2 cut(s) 219, 654
BstH2I RGCGCY 2 cut(s) 2806, 4299
BstHHI GCGC 7 cut(s) 219, 654, 1801, 2737, 2805, 2938, 4298
BstNI CCWGG 8 cut(s) 64, 735, 873, 2016, 2025, 2980, 3225, 4228
BstNSI RCATGY 3 cut(s) 2852, 3727, 4153
BstPI GGTNACC 1 cut(s) 2350
BstSCI CCNGG 9 cut(s) 62, 733, 871, 2014, 2023, 2592, 2978, 3223, 4226
BstSFI CTRYAG 2 cut(s) 825, 1399
BstSLI GKGCMC 3 cut(s) 451, 1285, 3271
BstUI CGCG 2 cut(s) 219, 654
BstV2I GAAGAC 5 cut(s) 546, 3021, 3755, 3981, 4336
BstX2I RGATCY 6 cut(s) 139, 281, 694, 2051, 2554, 3890
BstXI CCANNNNNNTGG 2 cut(s) 3862, 4543
BstYI RGATCY 6 cut(s) 139, 281, 694, 2051, 2554, 3890
BstZ17I GTATAC 1 cut(s) 1111
Bsu15I ATCGAT 1 cut(s) 4635
Bsu36I CCTNAGG 2 cut(s) 1608, 1852
BsuTUI ATCGAT 1 cut(s) 4635
BtgI CCRYGG 1 cut(s) 3300
BtgZI GCGATG 2 cut(s) 4500, 4679
BtrI CACGTC 1 cut(s) 2360
BtsI GCAGTG 4 cut(s) 449, 1408, 3254, 4107
BveI ACCTGC 3 cut(s) 818, 3673, 3773
CaiI CAGNNNCTG 5 cut(s) 647, 1561, 2126, 2324, 2623
CciI TCATGA 1 cut(s) 2068
CfoI GCGC 7 cut(s) 219, 654, 1801, 2737, 2805, 2938, 4298
Cfr10I RCCGGY 1 cut(s) 2033
Cfr13I GGNCC 7 cut(s) 66, 221, 533, 1354, 1655, 3072, 3852
ClaI ATCGAT 1 cut(s) 4635
Csp6I GTAC 3 cut(s) 483, 3727, 4470
CspCI CAANNNNNGTGG 6 cut(s) 990, 1025, 1135, 1170, 2346, 2381
CviQI GTAC 3 cut(s) 483, 3727, 4470
DraI TTTAAA 1 cut(s) 4657
DraIII CACNNNGTG 1 cut(s) 506
DriI GACNNNNNGTC 1 cut(s) 4286
EaeI YGGCCR 4 cut(s) 478, 2092, 3041, 4195
Eam1105I GACNNNNNGTC 1 cut(s) 4286
EciI GGCGGA 3 cut(s) 20, 1173, 4468
Ecl136II GAGCTC 2 cut(s) 3095, 3835
Eco130I CCWWGG 9 cut(s) 232, 622, 787, 881, 911, 2843, 3300, 3337, 4417
Eco147I AGGCCT 2 cut(s) 2227, 3712
Eco24I GRGCYC 2 cut(s) 3097, 3837
Eco31I GGTCTC 2 cut(s) 378, 2835
Eco47I GGWCC 5 cut(s) 66, 533, 1354, 1655, 3072
Eco47III AGCGCT 1 cut(s) 4297
Eco53kI GAGCTC 2 cut(s) 3095, 3835
Eco57I CTGAAG 6 cut(s) 558, 1761, 1956, 2532, 3188, 4284
Eco81I CCTNAGG 2 cut(s) 1608, 1852
Eco88I CYCGRG 4 cut(s) 666, 1201, 1575, 4077
Eco91I GGTNACC 1 cut(s) 2350
EcoICRI GAGCTC 2 cut(s) 3095, 3835
EcoO109I RGGNCCY 3 cut(s) 533, 1354, 3072
EcoO65I GGTNACC 1 cut(s) 2350
EcoRI GAATTC 1 cut(s) 1273
EcoRII CCWGG 8 cut(s) 62, 733, 871, 2014, 2023, 2978, 3223, 4226
EcoT14I CCWWGG 9 cut(s) 232, 622, 787, 881, 911, 2843, 3300, 3337, 4417
EcoT22I ATGCAT 2 cut(s) 2727, 2814
EcoT38I GRGCYC 2 cut(s) 3097, 3837
ErhI CCWWGG 9 cut(s) 232, 622, 787, 881, 911, 2843, 3300, 3337, 4417
Esp3I CGTCTC 1 cut(s) 4270
FaqI GGGAC 8 cut(s) 154, 1276, 1586, 1668, 1720, 2662, 4048, 4074
FauI CCCGC 2 cut(s) 3843, 4564
FauNDI CATATG 2 cut(s) 3814, 3828
FblI GTMKAC 3 cut(s) 411, 1110, 1671
FriOI GRGCYC 2 cut(s) 3097, 3837
FspAI RTGCGCAY 1 cut(s) 1800
FspBI CTAG 7 cut(s) 363, 882, 1341, 1869, 1920, 2466, 3482
FspI TGCGCA 2 cut(s) 1800, 2937
GlaI GCGC 7 cut(s) 218, 653, 1800, 2736, 2804, 2937, 4297
GsaI CCCAGC 1 cut(s) 3267
GsuI CTGGAG 6 cut(s) 108, 2079, 2591, 3921, 4098, 4356
HaeII RGCGCY 2 cut(s) 2806, 4299
HapII CCGG 5 cut(s) 2034, 2593, 3105, 4597, 4672
HhaI GCGC 7 cut(s) 219, 654, 1801, 2737, 2805, 2938, 4298
Hin6I GCGC 7 cut(s) 217, 652, 1799, 2735, 2803, 2936, 4296
HinP1I GCGC 7 cut(s) 217, 652, 1799, 2735, 2803, 2936, 4296
HincII GTYRAC 2 cut(s) 412, 3685
HindII GTYRAC 2 cut(s) 412, 3685
HindIII AAGCTT 5 cut(s) 920, 947, 1440, 1972, 2654
HpaII CCGG 5 cut(s) 2034, 2593, 3105, 4597, 4672
HphI GGTGA 7 cut(s) 1061, 1591, 2636, 2825, 2889, 3398, 3743
Hpy166II GTNNAC 6 cut(s) 412, 1111, 1283, 1672, 3685, 4414
Hpy8I GTNNAC 6 cut(s) 412, 1111, 1283, 1672, 3685, 4414
Hpy99I CGWCG 4 cut(s) 32, 339, 4555, 4570
HpyCH4III ACNGT 8 cut(s) 416, 602, 608, 638, 725, 2532, 2928, 3179
HpyCH4IV ACGT 2 cut(s) 2359, 2775
HpySE526I ACGT 2 cut(s) 2359, 2775
HspAI GCGC 7 cut(s) 217, 652, 1799, 2735, 2803, 2936, 4296
Kpn2I TCCGGA 1 cut(s) 3104
LguI GCTCTTC 3 cut(s) 3603, 3748, 3840
MaeI CTAG 7 cut(s) 363, 882, 1341, 1869, 1920, 2466, 3482
MaeII ACGT 2 cut(s) 2359, 2775
MbiI CCGCTC 3 cut(s) 3244, 3535, 3850
MfeI CAATTG 3 cut(s) 2363, 3362, 3447
MflI RGATCY 6 cut(s) 139, 281, 694, 2051, 2554, 3890
MhlI GDGCHC 7 cut(s) 451, 1285, 3088, 3097, 3271, 3313, 3837
MlsI TGGCCA 2 cut(s) 2094, 3043
MluNI TGGCCA 2 cut(s) 2094, 3043
MmeI TCCRAC 7 cut(s) 189, 1844, 2132, 2470, 2845, 3996, 4574
Mox20I TGGCCA 2 cut(s) 2094, 3043
Mph1103I ATGCAT 2 cut(s) 2727, 2814
MroI TCCGGA 1 cut(s) 3104
MroXI GAANNNNTTC 6 cut(s) 867, 2787, 2949, 3373, 3388, 4204
MscI TGGCCA 2 cut(s) 2094, 3043
MseI TTAA 7 cut(s) 1499, 1977, 2577, 3525, 4146, 4524, 4656
MslI CAYNNNNRTG 6 cut(s) 526, 705, 2481, 3081, 3441, 3525
Msp20I TGGCCA 2 cut(s) 2094, 3043
MspA1I CMGCKG 3 cut(s) 146, 893, 2452
MspCI CTTAAG 3 cut(s) 1498, 2576, 4523
MspI CCGG 5 cut(s) 2034, 2593, 3105, 4597, 4672
MspR9I CCNGG 9 cut(s) 64, 735, 873, 2016, 2025, 2594, 2980, 3225, 4228
MunI CAATTG 3 cut(s) 2363, 3362, 3447
Mva1269I GAATGC 1 cut(s) 3613
MvaI CCWGG 8 cut(s) 64, 735, 873, 2016, 2025, 2980, 3225, 4228
MvnI CGCG 2 cut(s) 219, 654
NciI CCSGG 1 cut(s) 2594
NcoI CCATGG 1 cut(s) 3300
NdeI CATATG 2 cut(s) 3814, 3828
NmuCI GTSAC 6 cut(s) 608, 2418, 2895, 3252, 3455, 3749
NsbI TGCGCA 2 cut(s) 1800, 2937
NsiI ATGCAT 2 cut(s) 2727, 2814
NspI RCATGY 3 cut(s) 2852, 3727, 4153
OliI CACNNNNGTG 1 cut(s) 705
PaeR7I CTCGAG 2 cut(s) 1201, 4077
PagI TCATGA 1 cut(s) 2068
PaqCI CACCTGC 1 cut(s) 818
PceI AGGCCT 2 cut(s) 2227, 3712
PciSI GCTCTTC 3 cut(s) 3603, 3748, 3840
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 3953
PctI GAATGC 1 cut(s) 3613
PdmI GAANNNNTTC 6 cut(s) 867, 2787, 2949, 3373, 3388, 4204
PflFI GACNNNGTC 1 cut(s) 2293
PflMI CCANNNNNTGG 3 cut(s) 740, 2258, 3356
PfoI TCCNGGA 2 cut(s) 62, 2014
PpuMI RGGWCCY 3 cut(s) 533, 1354, 3072
PshBI ATTAAT 1 cut(s) 3525
Psp124BI GAGCTC 2 cut(s) 3097, 3837
Psp1406I AACGTT 1 cut(s) 2775
Psp5II RGGWCCY 3 cut(s) 533, 1354, 3072
Psp6I CCWGG 8 cut(s) 62, 733, 871, 2014, 2023, 2978, 3223, 4226
PspEI GGTNACC 1 cut(s) 2350
PspFI CCCAGC 1 cut(s) 3263
PspGI CCWGG 8 cut(s) 62, 733, 871, 2014, 2023, 2978, 3223, 4226
PspPI GGNCC 7 cut(s) 66, 221, 533, 1354, 1655, 3072, 3852
PspPPI RGGWCCY 3 cut(s) 533, 1354, 3072
PspXI VCTCGAGB 1 cut(s) 1201
PsrI GAACNNNNNNTAC 2 cut(s) 622, 654
PstI CTGCAG 2 cut(s) 829, 1403
PstNI CAGNNNCTG 5 cut(s) 647, 1561, 2126, 2324, 2623
PsuI RGATCY 6 cut(s) 139, 281, 694, 2051, 2554, 3890
PsyI GACNNNGTC 1 cut(s) 2293
PvuII CAGCTG 2 cut(s) 146, 893
RsaI GTAC 3 cut(s) 484, 3728, 4471
RsaNI GTAC 3 cut(s) 483, 3727, 4470
RseI CAYNNNNRTG 6 cut(s) 526, 705, 2481, 3081, 3441, 3525
SacI GAGCTC 2 cut(s) 3097, 3837
SalI GTCGAC 1 cut(s) 410
SapI GCTCTTC 3 cut(s) 3603, 3748, 3840
SaqAI TTAA 7 cut(s) 1499, 1977, 2577, 3525, 4146, 4524, 4656
Sau96I GGNCC 7 cut(s) 66, 221, 533, 1354, 1655, 3072, 3852
ScrFI CCNGG 9 cut(s) 64, 735, 873, 2016, 2025, 2594, 2980, 3225, 4228
SduI GDGCHC 7 cut(s) 451, 1285, 3088, 3097, 3271, 3313, 3837
SfcI CTRYAG 2 cut(s) 825, 1399
Sfr274I CTCGAG 2 cut(s) 1201, 4077
SinI GGWCC 5 cut(s) 66, 533, 1354, 1655, 3072
SlaI CTCGAG 2 cut(s) 1201, 4077
SmiMI CAYNNNNRTG 6 cut(s) 526, 705, 2481, 3081, 3441, 3525
SmlI CTYRAG 9 cut(s) 1005, 1201, 1498, 1638, 2576, 4077, 4257, 4291, 4523
SmoI CTYRAG 9 cut(s) 1005, 1201, 1498, 1638, 2576, 4077, 4257, 4291, 4523
SseBI AGGCCT 2 cut(s) 2227, 3712
SspI AATATT 3 cut(s) 1810, 3562, 4267
SspMI CTAG 7 cut(s) 363, 882, 1341, 1869, 1920, 2466, 3482
SstI GAGCTC 2 cut(s) 3097, 3837
StuI AGGCCT 2 cut(s) 2227, 3712
StyD4I CCNGG 9 cut(s) 62, 733, 871, 2014, 2023, 2592, 2978, 3223, 4226
StyI CCWWGG 9 cut(s) 232, 622, 787, 881, 911, 2843, 3300, 3337, 4417
TaaI ACNGT 8 cut(s) 416, 602, 608, 638, 725, 2532, 2928, 3179
TaiI ACGT 2 cut(s) 2362, 2778
TaqII GACCGA 1 cut(s) 402
TatI WGTACW 1 cut(s) 3726
TauI GCSGC 3 cut(s) 222, 310, 3538
Tru1I TTAA 7 cut(s) 1499, 1977, 2577, 3525, 4146, 4524, 4656
Tru9I TTAA 7 cut(s) 1499, 1977, 2577, 3525, 4146, 4524, 4656
TseFI GTSAC 6 cut(s) 608, 2418, 2895, 3252, 3455, 3749
Tsp45I GTSAC 6 cut(s) 608, 2418, 2895, 3252, 3455, 3749
TspGWI ACGGA 4 cut(s) 1098, 3225, 3358, 4568
Tth111I GACNNNGTC 1 cut(s) 2293
Van91I CCANNNNNTGG 3 cut(s) 740, 2258, 3356
Vha464I CTTAAG 3 cut(s) 1498, 2576, 4523
VneI GTGCAC 1 cut(s) 1281
VpaK11BI GGWCC 5 cut(s) 66, 533, 1354, 1655, 3072
VspI ATTAAT 1 cut(s) 3525
XceI RCATGY 3 cut(s) 2852, 3727, 4153
XcmI CCANNNNNNNNNTGG 1 cut(s) 2885
XhoI CTCGAG 2 cut(s) 1201, 4077
XmaJI CCTAGG 1 cut(s) 881
XmiI GTMKAC 3 cut(s) 411, 1110, 1671
XmnI GAANNNNTTC 6 cut(s) 867, 2787, 2949, 3373, 3388, 4204
XspI CTAG 7 cut(s) 363, 882, 1341, 1869, 1920, 2466, 3482
Zsp2I ATGCAT 2 cut(s) 2727, 2814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.