Rh4CG315000

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005720
Physical Location & Seq
Forward (+)
57751917 .. 57759286
7370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG315000.1

Sequence Viewer

Length: 4587 bp
ATGGCGGAACGCAAGCTCGATCTCCCCGACGATCTCCTCTCCTCCAAACCCTCCGATCAGTCCTGGACCTCCAAAGTTGAAGTTTCTGGAGGAAATGATGAGGAAAAGGTGCTTATGGGGTCATCTGATGAGTTGAAAGATCCAGCAGCCTTGGATAGCAGCATACCTTTGTCACCCCAGTGGCTTTATGCCAAGCCGATTGAGTCTAAGTTGGAAATGCGTGGTCCAACTTCACTCGGAAACTCCACTGACAGCAACCAGAAGGACGGTTGGCGGTTGGAAGGATCTGAGGACAAGAAAGATTGGAGGCGACCTGCTACTGAAAGTGAAAGTAGCCGTCGTTGGCGTGAAGAGGAAAGAGAAACTAGCTTGCTTGGTGGTAGAAGAGATCGTAGGAAAACAGAACGCCGTGCTGACAATATTCCATTGAGGGACACAACTGACAGTAGAGCATTGCCTACAACTGACAGGTGGCATGATGGCCGCAATGAAGTACGGCGTGATAGCAAATGGTCGTCTAGGTGGGGTCCTGAAGACAAAGAAAAGGAGCCCCGAACTGAGAAGAGAACAGATGTGGAGAAGGATGATGCTCACAGTAACAGTGACAGTCAATCTCTTGGAGCTAACAGCCGTTCAGCTGCTGAGTCAGATTCTCGGGATAAATGGAGGCCACGACATAGAATGGAGGTTCAAACTGGGGGGTCAGCTACTTACCGTGCTGCACCTGGATTTGGACTAGAAAGAGGACGGGTGGAGGGTTCGAATTTGGGATTTACCCTGGGGCGAGGAAGGTCAAGTGGTATTGGGAGATCGGCAGGCACTATTGGCTCTGCTCTTTCAGGAAAGAGTGAAGGTGTCCCTGGGAAGCCAAGGCACTCAGCTGATGGTTTTTGCTACCCGAGGGGAAAGCTTCTTGATATTTATCGGCAGCGAAAGCCTGAACTCTCTTTTGATACCATGCCAGATGAGATGGAGGAGGCCCCCTCACTAACTCAAGTTGATTTTGTTAAACCATTGGCTTTTCATGCCCCTGATGCTGATGAGGAGGCCATCCTTAGCGATATATGGAAGGGAAAAATTACAAGTAGTGGGGTAGTATACAATTCATTCAGAAAGGGGAGATCAACTGAAAATATTACAGGTGTGGGAGACTTAGAAGCTGCTGATGGAGTTCTGGGCAATTTGCCCTCGACTGTCACTCAAGAGACTAGCACCTTTCAGGAGGCTGCAAATGCTGATGACTATGGGACTTTGTGGAATTATGGTTCACAGAGGAATGTGATAAATGGAAAAGATGTTGGTCATAAAGAAAGTGATAACAGAGCTACTGACGGAAAGGACCTTGGTGGAATTTCCCTATCAACTCAAAAAAGTAATGGTATCTATGGTGATGTTGAGACTGATGGTTCTTATGATAGTGCAAATCAGCTTCATGTTGGTGGCAATGGGAAAATTGGAGATTCTGCCTTTTCAAATCATCCTGTATCTGATGACATTGAGTTTGCCAATTATTCCGAAATAAGATCCAACCTTCCCGACGTTTCAAATACTCTCTATGGTTTGGCCTCTTCTGAGCAGAATGAGAATAGCAACCCTAGAGTGAAAGAGTTAGAAATGGGTGTTCATCCTGAGGGTTTGTATTACTACTACCTTGATCCTCAAGGGGTGACCCAAGGGCCATATCAGGGGTCAGACATCATTTCATGGTTTGAACAAGGTTTCTTTGGGATAGACTTGCTTGTTCGCTTGGAGGATGCTCCTGAAGGAACACCTTTCAAAGAGTTGGGGGAGTTCATGCCATATCTTAAATCTTGGGATGAGAATGGCAGTATCATAAATCCAAGTTCTAAGCTAGAAGAATCTGGTGGCTTAGGAGGGAGTCTGGAGTCCAGTTTACCCTTTTCTGCAGCAGTTTCAGACAGTAACTATGCATTTCTGGGAAATGACCATCAGCGGCCCATGCCTGAACTAAACAGCCTTTCATCTCAGCATATTCAGCCAAGAATTTCCGAGCCTGAAGCTCCACTACAACTACATTCAAGGGGTCAAAGCTTTAATGATTTTGTTGAACAAGATGAAGATATTGTGTTTCCTGGAATACATGGAACCGCTGCCTATTCTACTGCACGATCTTCTGGGAGCATTCACGATTCTATGGCAAACTCTATTAATCACCTTCCTCCCCCAACTGAAATGACAGAATCTGGTGTGCCTATTCAGAATGATAATAAGTTGCATCCCTTTGGCTTGCTATGGTCTGAGCTAGAAAGCGGCCAATCAAAGCATTCCAAGTCGGCCAATATGCCATCTAGTAAGGGGAGGGCTGTTCCATTTGGTGCAAATCCTGATCCAGCAATTGCTGAGACGTGGTCTGATCTTCATAGAAAAAGTTCAGTCTCTGATCCGAACTTGTATCAGGAAATGTTGACTCCTCGCCAATTGTCGCACATGGAACAGGAACCCAGTCATTATGATTTAGCAGAGCAGCTTATGTCGCAGCAAATTCGACAGCAGCAGCAACTCCAACAGCGAAATATGTTGTCTGGTTTTGCACACTTGAATGACAATGTATTAGATCCTTTGCAAAATCAGAATCTTATTCACCATCAACAGTTAGCCAACCATTCTGCAGCAGATCTGGATCACCTTTTGGCACTTCAGAGGCAGGCTCAGCTCGAGCAACATCAACTGCAACAACAGCAGCAGTTTCATCAACAGCAGAAGCTTTTACAGGAGCAACAGCAATCACAAGTACAACAGGTGCTTTTTGAACAATTGTTGCGTGGTCAAATGCATGATCCTACACTTAGGCAGCCACATGTTGATCCTATAAGAGCAAACAATGTTATTGATCAGGTTCTATTAGAGCAGCAGCTTCTACGTGAACTTCAGCAACGGTCTCACCATCTTCCAAGGCATGTTGATCCAACTATAGAGCGGTTAATTCAAGCAAAGTTTGGTCATACACCTCAAGGACATCAAACAGATTTGTTTGAGCTACTGTCTCGTGCACAGCATGAACAGATTCAGTCGCTGGAACATCAGATGCATGCCAGGCAGTTACCCATGGGAATAAGGCAGCGAATGGAAGAAGAGAGGCATATCGGTTCTGTCTGGCCAGCTGAGGAATCAAATCAGATTTTCAGGACCCATACTGGTAATCATGGTCACCGAGGGCACTCTTCTGGCTTTAATCCTTTAGATTTTTATCAGAGGCAGCAGAGGCCATCTCATGAGGAGCAGCTGAATCACCTTGACCGGAATCTTTCATTACAGGATCGACTTCAGCAAGGTTTTTATGAGCCTGGCTCACTGCCGTTTGAGCGGTCAATGTCATTACCTGCTGGTGCACCAGGGATGAACTTGGATGTAGTAAATGCTATGGCTCGTGCCCAGGGTTTAGATATGCAGGATTCCATTGGACGTATGCAGTCGGCTGGCCAGTCGGGTCAATTTTCGTCTGGAATCCCTTCCCACAATGCTCATCATCCTCATGTTCCTAACCAATTTCATGTTTCACATTTGGATGCAATTGAGGGACACTGGCCTGAGAAGAATGATGAGCTTGAGAATGACTGGATGGATGCTCGGTTTCAGCAGCTGCATATTAATGCTGAGAGGCAGAAAAGGGAATCAGAGATCAAGAATACTTCTCAGGATCAAAATTTATGGATGTCAGATGGAATTAATGATGAGAACTCAAAGAGACTACTAATGGATTTACTCCATCAAAAATCTGGCCATCAACCTAGCGAGTCGTTGAATGCGATGAGCAATGGGATGTTCTCGGATAAAAGACTGCCATCTGGCCACTACTCTGGATCAAGCTCCTCAAACCACCTGTTCAATCTCCATGCAGACCAAGAAGCAGGTCTAAATAACTCATTTCGTGTAGGGTCATTTGGTTCTAATCCAGGTGAACTCCTACAGGAAGAGCAGGCCAGCAGTGTGGAAAGCAATGAAAAGTTGATGTATGGATCTAATTCTGGAGCATTGGCTGATAGAGAGTCATTTCTGGCAGGAATGAATGCAACAAGCCAGTCACTTTACACAAATTCGAACATGTTAAGTAAGTCTTCTATAGGTAAAGAACTTTCTGAGTTGGAGGGCAGGAAGCGGGGGTCCAAAAGTGAGGGGATAATTATGGGTCGATCTTTTGAGACTCAAGAACGAATGGTTGAACAAGCAGGGTTGTCTGCAACAAATTTTGAGGAGATGTCAAAACACGCCCATAGCATGAATTCTTCCTCCGGTGTTTCAGGTGGGAATGCTGGTTTTTATGGTGACAAGATTGGCGGAAGTAATTCATTTGTCGAACAAACTGCCAAGGATCGGGCTCCCATCCCATCCAAAGGGCAAGAAAATATTCTGCTGAGACGACCTTCTGTCCCAAGTGCTTCTGCATCGCAAGAAGGATTATCAGAACTGATATCAGATCCAGTTCTTAGAGGAAAAAATTCGAGTGCTGCTTCAGATGGTATTTTATTTCTAACTCTACTAGTTTCTTATCTTGTATGGTTACATTTTGTTGGAGGGGGAGTGGTGACCGACGTTTCAATCAAATTATCAAATTATACTTGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1528

Amino Acids

169.7

Weight (kDa)

5.42

Isoelectric Point (pI)

55.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 548 - 593 2.8e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 322, 3349, 3860
AccBSI CCGCTC 2 cut(s) 2938, 3325
AccI GTMKAC 1 cut(s) 1098
AcoI YGGCCR 7 cut(s) 481, 2272, 2295, 3116, 3439, 3739, 3808
AcsI RAATTY 9 cut(s) 763, 1350, 2004, 2502, 3664, 4054, 4204, 4240, 4456
AcuI CTGAAG 7 cut(s) 552, 1782, 2037, 2642, 2873, 3269, 4455
AfaI GTAC 2 cut(s) 495, 2754
AfiI CCNNNNNNNGG 4 cut(s) 731, 1685, 4332, 4352
AflIII ACRYGT 2 cut(s) 2818, 4062
AhdI GACNNNNNGTC 1 cut(s) 4385
AhlI ACTAGT 1 cut(s) 4498
AjiI CACGTC 1 cut(s) 2367
AleI CACNNNNGTG 1 cut(s) 178
AloI GAACNNNNNNTCC 2 cut(s) 1249, 1281
Alw21I GWGCWC 2 cut(s) 3013, 3352
Alw44I GTGCAC 2 cut(s) 3009, 3348
AlwNI CAGNNNCTG 5 cut(s) 641, 2204, 2399, 3034, 3601
Ama87I CYCGRG 3 cut(s) 654, 898, 2675
ApaLI GTGCAC 2 cut(s) 3009, 3348
ApoI RAATTY 9 cut(s) 763, 1350, 2004, 2502, 3664, 4054, 4204, 4240, 4456
ArsI GACNNNNNNTTYG 2 cut(s) 1484, 1516
AseI ATTAAT 3 cut(s) 2169, 3609, 3687
Asp700I GAANNNNTTC 6 cut(s) 2389, 3024, 3469, 4303, 4365, 4456
AspS9I GGNCC 9 cut(s) 66, 224, 527, 979, 1339, 1676, 1956, 3147, 4122
AsuII TTCGAA 2 cut(s) 761, 4058
AvaI CYCGRG 3 cut(s) 654, 898, 2675
AvaII GGWCC 6 cut(s) 66, 224, 527, 1339, 3147, 4122
AxyI CCTNAGG 1 cut(s) 1629
BaeGI GKGCMC 4 cut(s) 3013, 3180, 3352, 3394
BalI TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
BanII GRGCYC 2 cut(s) 552, 4339
BarI GAAGNNNNNNTAC 2 cut(s) 2010, 2042
BauI CACGAG 2 cut(s) 3006, 3387
BbsI GAAGAC 2 cut(s) 540, 4068
Bbv12I GWGCWC 2 cut(s) 3013, 3352
BbvCI CCTCAGC 1 cut(s) 3123
BceAI ACGGC 5 cut(s) 321, 393, 512, 615, 3301
BcgI CGANNNNNNTGC 4 cut(s) 2486, 2520, 4304, 4338
BclI TGATCA 1 cut(s) 2851
BcuI ACTAGT 1 cut(s) 4498
BfmI CTRYAG 5 cut(s) 1905, 2628, 2931, 3926, 4080
BfuAI ACCTGC 3 cut(s) 322, 3349, 3860
BglII AGATCT 1 cut(s) 2635
BlpI GCTNAGC 1 cut(s) 2670
Bme18I GGWCC 6 cut(s) 66, 224, 527, 1339, 3147, 4122
BmeRI GACNNNNNGTC 1 cut(s) 4385
BmeT110I CYCGRG 3 cut(s) 654, 898, 2675
BmgBI CACGTC 1 cut(s) 2367
BmgT120I GGNCC 9 cut(s) 66, 224, 527, 979, 1339, 1676, 1956, 3147, 4122
BmiI GGNNCC 8 cut(s) 528, 549, 981, 2107, 2460, 3149, 4123, 4338
BmrI ACTGGG 3 cut(s) 172, 705, 2457
BmsI GCATC 8 cut(s) 577, 1024, 1744, 2245, 3036, 3517, 3574, 4412
BmuI ACTGGG 3 cut(s) 172, 705, 2457
BpiI GAAGAC 2 cut(s) 540, 4068
BplI GAGNNNNNCTC 8 cut(s) 968, 1000, 2653, 2685, 3214, 3246, 3293, 3325
BpmI CTGGAG 3 cut(s) 108, 1904, 4008
Bpu10I CCTNAGC 3 cut(s) 1055, 1870, 3123
Bpu1102I GCTNAGC 1 cut(s) 2670
Bpu14I TTCGAA 2 cut(s) 761, 4058
BpuEI CTTGAG 6 cut(s) 978, 1185, 1644, 2955, 3587, 4149
BsaAI YACGTR 1 cut(s) 2882
BsaBI GATNNNNATC 3 cut(s) 921, 2640, 3207
BsaI GGTCTC 1 cut(s) 2904
BsaWI WCCGGW 2 cut(s) 3258, 4250
BsaXI ACNNNNNCTCC 2 cut(s) 4524, 4554
Bsc4I CCNNNNNNNGG 4 cut(s) 731, 1685, 4332, 4352
Bse21I CCTNAGG 1 cut(s) 1629
Bse3DI GCAATG 5 cut(s) 452, 493, 1450, 3781, 3964
Bse8I GATNNNNATC 3 cut(s) 921, 2640, 3207
BseJI GATNNNNATC 3 cut(s) 921, 2640, 3207
BseLI CCNNNNNNNGG 4 cut(s) 731, 1685, 4332, 4352
BseMI GCAATG 5 cut(s) 452, 493, 1450, 3781, 3964
BseRI GAGGAG 8 cut(s) 26, 31, 989, 1058, 2421, 3251, 3820, 4226
BseSI GKGCMC 4 cut(s) 3013, 3180, 3352, 3394
BsgI GTGCAG 2 cut(s) 705, 2109
BsiHKAI GWGCWC 2 cut(s) 3013, 3352
BsiHKCI CYCGRG 3 cut(s) 654, 898, 2675
BsiSI CCGG 2 cut(s) 3259, 4251
BslFI GGGAC 5 cut(s) 446, 842, 1261, 3552, 4373
BslI CCNNNNNNNGG 4 cut(s) 731, 1685, 4332, 4352
BsmBI CGTCTC 2 cut(s) 2357, 4369
BsmFI GGGAC 5 cut(s) 446, 842, 1261, 3552, 4373
BsmI GAATGC 5 cut(s) 2142, 2284, 3769, 4033, 4273
Bso31I GGTCTC 1 cut(s) 2904
BsoBI CYCGRG 3 cut(s) 654, 898, 2675
Bsp119I TTCGAA 2 cut(s) 761, 4058
Bsp1286I GDGCHC 6 cut(s) 552, 3013, 3180, 3352, 3394, 4339
Bsp1720I GCTNAGC 1 cut(s) 2670
Bsp19I CCATGG 1 cut(s) 3066
BspHI TCATGA 1 cut(s) 3232
BspLI GGNNCC 8 cut(s) 528, 549, 981, 2107, 2460, 3149, 4123, 4338
BspMAI CTGCAG 2 cut(s) 1909, 2632
BspMI ACCTGC 3 cut(s) 322, 3349, 3860
BspQI GCTCTTC 1 cut(s) 3927
BspT104I TTCGAA 2 cut(s) 761, 4058
BspTNI GGTCTC 1 cut(s) 2904
BsrBI CCGCTC 2 cut(s) 2938, 3325
BsrDI GCAATG 5 cut(s) 452, 493, 1450, 3781, 3964
BssNAI GTATAC 1 cut(s) 1099
BssSI CACGAG 2 cut(s) 3006, 3387
BssT1I CCWWGG 7 cut(s) 150, 869, 1342, 1672, 2912, 3066, 4326
Bst1107I GTATAC 1 cut(s) 1099
Bst2BI CACGAG 2 cut(s) 3006, 3387
Bst6I CTCTTC 7 cut(s) 345, 379, 557, 1573, 3087, 3187, 3927
BstBAI YACGTR 1 cut(s) 2882
BstBI TTCGAA 2 cut(s) 761, 4058
BstDSI CCRYGG 1 cut(s) 3066
BstEII GGTNACC 3 cut(s) 1666, 3167, 4543
BstNSI RCATGY 4 cut(s) 2822, 2921, 3053, 4066
BstPI GGTNACC 3 cut(s) 1666, 3167, 4543
BstSFI CTRYAG 5 cut(s) 1905, 2628, 2931, 3926, 4080
BstSLI GKGCMC 4 cut(s) 3013, 3180, 3352, 3394
BstV2I GAAGAC 2 cut(s) 540, 4068
BstX2I RGATCY 7 cut(s) 139, 284, 1523, 2575, 2635, 3977, 4435
BstXI CCANNNNNNTGG 2 cut(s) 3818, 3949
BstYI RGATCY 7 cut(s) 139, 284, 1523, 2575, 2635, 3977, 4435
BstZ17I GTATAC 1 cut(s) 1099
Bsu36I CCTNAGG 1 cut(s) 1629
BtgI CCRYGG 1 cut(s) 3066
BtgZI GCGATG 2 cut(s) 3782, 4389
BtrI CACGTC 1 cut(s) 2367
BtsI GCAGTG 2 cut(s) 3311, 3952
BtsIMutI CAGTG 6 cut(s) 185, 246, 607, 3311, 3541, 3952
BveI ACCTGC 3 cut(s) 322, 3349, 3860
CaiI CAGNNNCTG 5 cut(s) 641, 2204, 2399, 3034, 3601
CciI TCATGA 1 cut(s) 3232
Cfr13I GGNCC 9 cut(s) 66, 224, 527, 979, 1339, 1676, 1956, 3147, 4122
Csp6I GTAC 2 cut(s) 494, 2753
CviQI GTAC 2 cut(s) 494, 2753
DriI GACNNNNNGTC 1 cut(s) 4385
EaeI YGGCCR 7 cut(s) 481, 2272, 2295, 3116, 3439, 3739, 3808
Eam1104I CTCTTC 7 cut(s) 345, 379, 557, 1573, 3087, 3187, 3927
Eam1105I GACNNNNNGTC 1 cut(s) 4385
EarI CTCTTC 7 cut(s) 345, 379, 557, 1573, 3087, 3187, 3927
EciI GGCGGA 2 cut(s) 20, 4311
Eco130I CCWWGG 7 cut(s) 150, 869, 1342, 1672, 2912, 3066, 4326
Eco24I GRGCYC 2 cut(s) 552, 4339
Eco31I GGTCTC 1 cut(s) 2904
Eco32I GATATC 1 cut(s) 4431
Eco47I GGWCC 6 cut(s) 66, 224, 527, 1339, 3147, 4122
Eco57I CTGAAG 7 cut(s) 552, 1782, 2037, 2642, 2873, 3269, 4455
Eco81I CCTNAGG 1 cut(s) 1629
Eco88I CYCGRG 3 cut(s) 654, 898, 2675
Eco91I GGTNACC 3 cut(s) 1666, 3167, 4543
EcoO109I RGGNCCY 4 cut(s) 527, 979, 1339, 3147
EcoO65I GGTNACC 3 cut(s) 1666, 3167, 4543
EcoRI GAATTC 1 cut(s) 4240
EcoRV GATATC 1 cut(s) 4431
EcoT14I CCWWGG 7 cut(s) 150, 869, 1342, 1672, 2912, 3066, 4326
EcoT22I ATGCAT 3 cut(s) 1933, 2796, 3051
EcoT38I GRGCYC 2 cut(s) 552, 4339
ErhI CCWWGG 7 cut(s) 150, 869, 1342, 1672, 2912, 3066, 4326
Esp3I CGTCTC 2 cut(s) 2357, 4369
FalI AAGNNNNNCTT 2 cut(s) 4060, 4092
FaqI GGGAC 5 cut(s) 446, 842, 1261, 3552, 4373
FauI CCCGC 1 cut(s) 4110
FbaI TGATCA 1 cut(s) 2851
FblI GTMKAC 1 cut(s) 1098
FriOI GRGCYC 2 cut(s) 552, 4339
GsuI CTGGAG 3 cut(s) 108, 1904, 4008
HapII CCGG 2 cut(s) 3259, 4251
HincII GTYRAC 1 cut(s) 2427
HindII GTYRAC 1 cut(s) 2427
HindIII AAGCTT 3 cut(s) 908, 2050, 2723
HpaII CCGG 2 cut(s) 3259, 4251
Hpy166II GTNNAC 8 cut(s) 1099, 1268, 1895, 2427, 2885, 3011, 3350, 3920
Hpy8I GTNNAC 8 cut(s) 1099, 1268, 1895, 2427, 2885, 3011, 3350, 3920
Hpy99I CGWCG 4 cut(s) 32, 342, 1541, 4553
HpyCH4IV ACGT 5 cut(s) 1539, 2366, 2881, 3424, 4551
HpySE526I ACGT 5 cut(s) 1539, 2366, 2881, 3424, 4551
Ksp22I TGATCA 1 cut(s) 2851
LguI GCTCTTC 1 cut(s) 3927
LweI GCATC 8 cut(s) 577, 1024, 1744, 2245, 3036, 3517, 3574, 4412
MaeII ACGT 5 cut(s) 1539, 2366, 2881, 3424, 4551
MbiI CCGCTC 2 cut(s) 2938, 3325
MfeI CAATTG 4 cut(s) 2355, 2438, 2774, 3531
MflI RGATCY 7 cut(s) 139, 284, 1523, 2575, 2635, 3977, 4435
MhlI GDGCHC 6 cut(s) 552, 3013, 3180, 3352, 3394, 4339
MlsI TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
MluNI TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
MlyI GAGTC 8 cut(s) 212, 653, 1888, 1895, 2422, 3764, 4016, 4156
MmeI TCCRAC 8 cut(s) 192, 251, 258, 1551, 2548, 2951, 4083, 4510
Mox20I TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
Mph1103I ATGCAT 3 cut(s) 1933, 2796, 3051
MroXI GAANNNNTTC 6 cut(s) 2389, 3024, 3469, 4303, 4365, 4456
MscI TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
MseI TTAA 9 cut(s) 1008, 1806, 2055, 2169, 2942, 3192, 3609, 3687, 4067
MslI CAYNNNNRTG 6 cut(s) 178, 1437, 2559, 3492, 3525, 3609
Msp20I TGGCCA 4 cut(s) 3118, 3441, 3741, 3810
MspA1I CMGCKG 7 cut(s) 638, 881, 1954, 2111, 3122, 3244, 3601
MspI CCGG 2 cut(s) 3259, 4251
MunI CAATTG 4 cut(s) 2355, 2438, 2774, 3531
Mva1269I GAATGC 5 cut(s) 2142, 2284, 3769, 4033, 4273
NcoI CCATGG 1 cut(s) 3066
NlaIV GGNNCC 8 cut(s) 528, 549, 981, 2107, 2460, 3149, 4123, 4338
NmuCI GTSAC 8 cut(s) 171, 602, 1195, 1666, 3167, 4041, 4283, 4543
NsiI ATGCAT 3 cut(s) 1933, 2796, 3051
NspI RCATGY 4 cut(s) 2822, 2921, 3053, 4066
NspV TTCGAA 2 cut(s) 761, 4058
OliI CACNNNNGTG 1 cut(s) 178
PaeI GCATGC 1 cut(s) 3053
PaeR7I CTCGAG 1 cut(s) 2675
PagI TCATGA 1 cut(s) 3232
PasI CCCWGGG 3 cut(s) 778, 860, 3394
PciI ACATGT 2 cut(s) 2818, 4062
PciSI GCTCTTC 1 cut(s) 3927
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 3764
PctI GAATGC 5 cut(s) 2142, 2284, 3769, 4033, 4273
PdmI GAANNNNTTC 6 cut(s) 2389, 3024, 3469, 4303, 4365, 4456
PflFI GACNNNGTC 1 cut(s) 2368
PfoI TCCNGGA 2 cut(s) 62, 2092
PleI GAGTC 8 cut(s) 211, 652, 1887, 1894, 2422, 3763, 4015, 4156
PpsI GAGTC 8 cut(s) 211, 652, 1887, 1894, 2422, 3763, 4015, 4156
Ppu21I YACGTR 1 cut(s) 2882
PpuMI RGGWCCY 3 cut(s) 527, 1339, 3147
PscI ACATGT 2 cut(s) 2818, 4062
PshBI ATTAAT 3 cut(s) 2169, 3609, 3687
Psp5II RGGWCCY 3 cut(s) 527, 1339, 3147
PspEI GGTNACC 3 cut(s) 1666, 3167, 4543
PspN4I GGNNCC 8 cut(s) 528, 549, 981, 2107, 2460, 3149, 4123, 4338
PspPI GGNCC 9 cut(s) 66, 224, 527, 979, 1339, 1676, 1956, 3147, 4122
PspPPI RGGWCCY 3 cut(s) 527, 1339, 3147
PspXI VCTCGAGB 1 cut(s) 2675
PstI CTGCAG 2 cut(s) 1909, 2632
PstNI CAGNNNCTG 5 cut(s) 641, 2204, 2399, 3034, 3601
PsuI RGATCY 7 cut(s) 139, 284, 1523, 2575, 2635, 3977, 4435
PsyI GACNNNGTC 1 cut(s) 2368
PvuII CAGCTG 5 cut(s) 638, 881, 3122, 3244, 3601
RsaI GTAC 2 cut(s) 495, 2754
RsaNI GTAC 2 cut(s) 494, 2753
RseI CAYNNNNRTG 6 cut(s) 178, 1437, 2559, 3492, 3525, 3609
SapI GCTCTTC 1 cut(s) 3927
SaqAI TTAA 9 cut(s) 1008, 1806, 2055, 2169, 2942, 3192, 3609, 3687, 4067
Sau96I GGNCC 9 cut(s) 66, 224, 527, 979, 1339, 1676, 1956, 3147, 4122
SchI GAGTC 8 cut(s) 212, 653, 1888, 1895, 2422, 3764, 4016, 4156
SduI GDGCHC 6 cut(s) 552, 3013, 3180, 3352, 3394, 4339
SfaNI GCATC 8 cut(s) 577, 1024, 1744, 2245, 3036, 3517, 3574, 4412
SfcI CTRYAG 5 cut(s) 1905, 2628, 2931, 3926, 4080
Sfr274I CTCGAG 1 cut(s) 2675
SfuI TTCGAA 2 cut(s) 761, 4058
SinI GGWCC 6 cut(s) 66, 224, 527, 1339, 3147, 4122
SlaI CTCGAG 1 cut(s) 2675
SmiMI CAYNNNNRTG 6 cut(s) 178, 1437, 2559, 3492, 3525, 3609
SmlI CTYRAG 7 cut(s) 993, 1200, 1659, 2675, 2970, 3566, 4164
SmoI CTYRAG 7 cut(s) 993, 1200, 1659, 2675, 2970, 3566, 4164
SpeI ACTAGT 1 cut(s) 4498
SphI GCATGC 1 cut(s) 3053
SspI AATATT 3 cut(s) 421, 1135, 4366
StyI CCWWGG 7 cut(s) 150, 869, 1342, 1672, 2912, 3066, 4326
TaiI ACGT 5 cut(s) 1542, 2369, 2884, 3427, 4554
TaqII GACCGA 1 cut(s) 4562
TatI WGTACW 1 cut(s) 2752
TauI GCSGC 3 cut(s) 486, 1957, 2274
Tru1I TTAA 9 cut(s) 1008, 1806, 2055, 2169, 2942, 3192, 3609, 3687, 4067
Tru9I TTAA 9 cut(s) 1008, 1806, 2055, 2169, 2942, 3192, 3609, 3687, 4067
TscAI CASTG 6 cut(s) 185, 253, 607, 3318, 3548, 3952
TseFI GTSAC 8 cut(s) 171, 602, 1195, 1666, 3167, 4041, 4283, 4543
Tsp45I GTSAC 8 cut(s) 171, 602, 1195, 1666, 3167, 4041, 4283, 4543
TspGWI ACGGA 1 cut(s) 1347
TspRI CASTG 6 cut(s) 185, 253, 607, 3318, 3548, 3952
Tth111I GACNNNGTC 1 cut(s) 2368
VneI GTGCAC 2 cut(s) 3009, 3348
VpaK11BI GGWCC 6 cut(s) 66, 224, 527, 1339, 3147, 4122
VspI ATTAAT 3 cut(s) 2169, 3609, 3687
XapI RAATTY 9 cut(s) 763, 1350, 2004, 2502, 3664, 4054, 4204, 4240, 4456
XceI RCATGY 4 cut(s) 2822, 2921, 3053, 4066
XcmI CCANNNNNNNNNTGG 1 cut(s) 3734
XhoI CTCGAG 1 cut(s) 2675
XmiI GTMKAC 1 cut(s) 1098
XmnI GAANNNNTTC 6 cut(s) 2389, 3024, 3469, 4303, 4365, 4456
Zsp2I ATGCAT 3 cut(s) 1933, 2796, 3051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.