MD13G1123200.v1.1

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005720
Physical Location & Seq
Reverse (-)
9132172 .. 9139657
7486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1123200.v1.1.491

Sequence Viewer

Length: 4749 bp
ATGGCGGAAGGCAAGCTCGATCTTCCCGACGATCTCCTCTCCTGTAAGCCGTCCGATCAGTCCTGGACCTCCAAAGTTGAAGCCTCTGGAGGAAATGATGAAAAAAAGGTGCTTATCGGGTCATCTGATGACTCAAAAGATCCAGCTGCCTCAGAGAGCAGCATACCTTTGTCCCCCCAGTGGCTTTATGCCAAACCCAGTGAGTCTAAGTTGGAAATGCGTGGCCCAAGTTCCCTCGGAAACTCTGCTGATAGCAACCAGAAGGAAGGTTGGCGTTTGGAAGGATCTGATGACAAAAAAGATTGGAGGCGGCCTGCCGCTGAAAGTGAAAATAGCCGTCGTTGGCGTGAAGAGGAAAGAGAAACTAGCTTGCTTGGTGGTAGACGAGACCGAAGGAAACCAGAACGCCGTGTTGACAGTGTTCCAGTGAGAGACACAACTGACAATAGGCCACTGCCTGCATCTGAAAGGTGGCATGATGGCCGTACCTCTGCACATGAAGCACGACGTGATAGCAAATGGTCATTGAGGTGGGGTCCTGAAGACAAAGAAAAGGAGTCTCGAACTGAAAAGAAAACAGATATGGAGAAGGAAGATAATCACATTAACAATGACAACCAATCCCTTGGTGGTAATAACCGTTCAGCTTCTGAGCGTGAGTCAGATTCTCGGGATAAATGGAGGCCACGCCATAGGGATGTTCACTCCGGTGGATCAAATTCCTACCGTGCTGCACCTGGATTTGGAACTGAAAAAGGAAGGGTAGAGGGTTCAAATTTGGGATTTACCCTTGGAAGAGGGAGGGCATCTGGTGTTGGGAGATCATCTGCAGGCGTTATTGGCTCTACTTCTTCTGGAAAAATTGGAAGTGTCCCTGGTAAACCTAGGCACTCAGCTGATAGTTTTTGTTACCCAAGGGGAAAGCTTCTTGACATTTATCGTCAGCGAAAGCTTGATCTGTCATTTGCTACCATGCCAGATGACATGGAAGAATCGCCCCCTACAACTGAAGTAGGCTTTATGGAACCATTAGCTTTTCTTGCCCCTGATGGCGAAGAGGAGGCCATCCTTAGTGACATATGGAAGGGAAAGATTACAAGTAGTGGGGTAGTATACAATTCATTTAGAAAGGGAAGATCAACTGAAAACAGTACAGGTGGGGGAGACTTGGAAGCTGTTGATGGAGTTCTGGGTATTTTACCCAATATCTTGCAGGAGGCTGCAAATGCTGATGATTATGGGACTTCACAGAACTCTGGTGCACAAATGAATGTGCTAGATGACAAAGATGCCAATCATAAAGAATGGGAAACAAAATCTAGTCCAGGAAAGGACCTTGATGGATTGTCTTTAACATTTCAGAAAACTAATGGTATCTGCAGTGATGTTGAGACTGAAGATGGCAGTAGGCAAATTGCAGATTCTTCTTTTTCCGGCCATTCTTTGTCTAATGACATTGAGTTTGGCACTTCTGACATAAAATCAAAACTTCCTGATGATTCAAATACTCTATATGCTCTTGGCTCATCTGAGCAGAATCAGAATAACAACTGTCGGGTGAAAGAGTTAGAAGGGGACGTCCCTCCTGAGGATTTATGTTTGTATTACCTTGATCCTCAAGGGGTGGTTCAGGGACCATATTTGGGGGTAGACATTATTTCATGGTTTGAACAAGGGTTTTTTGGGACAAACTTGCTTGTTCGCTTGGCAGATACTCCTGAAGCAACACCTTTCAAAGAATTGGGAGAGGTCATGCCACATCTGAAAGTTTGGGATGGGCATAGGAGTATTGTAAATCCGACTTCTAATATAGAAGAATCAAGTAGCTCCTTAGGGAACATAGAGTCTAACTTACCTTCTTCGGCTCCAGTTTCAGAAATGACTACTTCATTTGGAGGGAATGACTTAAGGCGGCCGTTGCCTGAACTAAACAGCCTTTTAGCTCAGCATATTCAACTTAGAAATTCTGAGCATGAAGCTCCAATACAACTGCCACATTCCAGGGGTCAAAGCTTTAATGATTTTGATGCCGAAGATGAAGATATCGTGTTTCCAGGAATACCTGGAACCACCAGCTATTCTACTGCAAGATCTTCTGGAACCATTCATGATTCTATTGCAAATTCTATTGGCCACCTTCCTCCAACTGAGTTGACGGAATCCGGTGTGCCCATTCAGAATGATAACAAGTTGCACCCCTTTGGCTTGCTTTGGTCTGAGCTAGAAGGCAGTCAAACGAAACATGCCAAGCCAGCCAATACGCCTTCTAGCATGGGGAGGGCTGTTCAATTTGGTGCAATTTCTGATCCAGCAGTTCTTGCTGATGCATGGTCCGATGTTCATAGAAAAAATTCAGTCTCTGATACAAACCTCTATCAGGACATGGTAGCTCCTCGCCAATTGGCACACATGAAACAGGAACCCAATCATTTTGATTTAGCTGAGCTGCTTGCACCACAGCAAATTCGACAGCAGCAGCACCAACAGCGGAATATTTTGTCTAGTTTTGGACACTTGAATGATGCAGCATTAGAGCATTTGCCAAGTCAGAATCTTATCCACCAACAGTTAGCCAACCATTCTTCAGCAGATCTGGATCACCTTTTGGCACTTCAGATGCAACAGCACCGGGAGGCTCAGCTCCAGCAACATCATCAACTGCAACAGCAGCAGTTTCATCAACAGCAGAAGCTTTTACAGGAGCAACAGTCCCAAGTACAACAGGTGCTTCTGGAACAACTACTGCGTGGTCAAATGCATGATCCTGCGCTTAGGCAGCCACACGTTGATCCTGTCAGAGCAAACAACGTTCTTGAACAGGTTCTATTAGAGCAGCGCCGTCTGCATGAACTCCAACAACGGTCTCACCATTTTCCAAGGCATGTTGATCCATCTTTGGAGCAGCTTATCCAAGCGAAGTTTGGTCAATCATCACCACAAGGACATCAAGCAGATTTTCTTGATCTACTGTCTCGTGCGCAGCATGAACAAATTCAATCATTGGAAGATCAGATGCAAGCCAGGCAGTTACCAGTGGGAATGAGGCAGAGGGTGGAAGAAGAGAGACACGGTGGTGCCGTCTGGCCAGCGGATGAATCAAATCAGTTTTTGAGGACCCATGGTGGTGCTCAGCGAGCTCACTCTTCCGGATTTAGCCCTTTAGATTTTTATCAGAGGCAACAGAGGCCATCCCATGAGGAGCAGCTTAGTCAACTTGACCGTAATCTTTCATTGCAGGATCGACTTCAGCAAGGGTTTTATGAGCCTGGCTCTCATCCGTTTGAGCAGTCTATGTCACTGCCTGCTGGGGCACAAGGGATGAACTTGGATGCTGTAAATGCTATGGCTCGTGCTCAGGGTTTGGATATGCATGATTCCCTTGGACGGATGCCATCGGGTGGTCAATTGGGAACATTTTCATCCGGCATCCATTCTCATAATCCTCATCACCCTCTTCCTAACCAATTTCATGCTTCACATTTGGATGCAATTGAGGGTCACTGGACCGAGAAGAATGAACACCTAGACAATGATTGGATGGACTCTCGGTTTCAGCAGTTGCATATTAATGCTGAGCGGCAGAAAAGGGAGTCAGAAATCAAAATTCCTTCTCAAGATCGAGCTTTATTTATGTCAGATGGATTGAATGAAGAGCATTCAAAGAGACTATTGATGGAGTTACTCCATAAAAAATCAGGCCATCAACCTGCCGAGTCATTGAATGTCAATAATGGCATGTTCTCTGATAAAAGGCCGTCATCTGGCATGTACTCTGGTTCAAGCTCTTCTAATCACCCATTTAGCCTTCATGCAGACCATGAAGCAGGTCTAAATAATTCATTTCGAGTAGGATCCTATGGTTCAGATCATATGGAACTCCCACAGGAAGAGCGGGCCAGCAGCGTGGAAAGCAATGAAAAGCTGATGCACAGATCTGATTCTGGAGCAATGGTTGAGAGAGAGTCATTCTTGGATGGCATTAATGCTACAACTCAGCCAATTTACACAAATTCTAACATGATTATCAAGTCTTCTATCAATAAAGAGGTTTTGGAGTTGGACGGGTGGAAGCGTGGGACGAAGAGTGAGGGGATCATTAGGGGCCAAGCGTTTGATATTCGAGAACGAATGGTCGAACAAGCAGGGTTGGGTGCTCCAGATTATGAGGAGAGGTCAGCAATTGCCCTTAACATGCGATCGTCATCTGGTGTTTCAGGTGGTAATATAGGCTTTCATAGCGACAAGATTGGCCGAAGTAATTCATTTGCGGAAGAAACGACCAGGGAACGGTTTCCAGCCCCATCCAAAAGTCAAGATAATATTCTGCTGAGACGACCTCCTGTCTCAAGTGTTTCAGCATCCCAAGAAGGATTGTCAGAACTGCTCCCTAATCCAGTCTTCCGAGGAAAAAGTGCAAGTGCTGCTCCAGATGGGGGGAGGCAAGATCCAGTAAACCACGGTTCTGATGCCTTGCCGCCATCTTCAAAGAAAGAAATGCATTTTCGCCATACTTCATCCACCAGCGATGCTGATGTGTCAGAGGCATCGTTTATGGATATGCTTAAGAGCAATACCAAGAAGACAGCTCCGATGGATGCCCATGCGGCAGCAGGAGAAGATGCAATGCAAGGGAACCGAAGCGGCAAAAAGAAGGGGAAGAAAGGGAGGCAAATTGATCCCGCTCTCCTCGGTTTTAAAGTTACCAGCAATCGGATAATGAGGGGCGAAATCCAACGCATGGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1583

Amino Acids

175.34

Weight (kDa)

5.66

Isoelectric Point (pI)

58.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 535 - 579 9.2e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1581
Acc16I TGCGCA 1 cut(s) 2979
Acc36I ACCTGC 2 cut(s) 3715, 3815
AccB1I GGYRCC 1 cut(s) 3074
AccB7I CCANNNNNTGG 2 cut(s) 3398, 4738
AccBSI CCGCTC 3 cut(s) 3577, 3892, 4682
AccI GTMKAC 3 cut(s) 382, 1113, 1650
AccIII TCCGGA 1 cut(s) 3146
AclI AACGTT 1 cut(s) 2808
AcoI YGGCCR 6 cut(s) 481, 1435, 1913, 2131, 3083, 4249
AcsI RAATTY 9 cut(s) 718, 775, 1963, 2122, 2350, 2463, 2991, 3603, 4009
AcuI CTGAAG 7 cut(s) 561, 1029, 1416, 1740, 2568, 2597, 3230
AcyI GRCGYC 1 cut(s) 1578
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 4 cut(s) 487, 1153, 2718, 3770
AflII CTTAAG 2 cut(s) 1906, 4562
AflIII ACRYGT 1 cut(s) 2782
AhdI GACNNNNNGTC 1 cut(s) 4340
AjiI CACGTC 1 cut(s) 509
AleI CACNNNNGTG 2 cut(s) 708, 3072
Alw21I GWGCWC 5 cut(s) 1264, 3130, 3139, 3355, 4156
Alw44I GTGCAC 1 cut(s) 1260
AlwNI CAGNNNCTG 2 cut(s) 650, 2360
Ama87I CYCGRG 1 cut(s) 669
Aor13HI TCCGGA 1 cut(s) 3146
ApaLI GTGCAC 1 cut(s) 1260
ApoI RAATTY 9 cut(s) 718, 775, 1963, 2122, 2350, 2463, 2991, 3603, 4009
ArsI GACNNNNNNTTYG 2 cut(s) 1445, 1477
AseI ATTAAT 2 cut(s) 3567, 3981
Asp700I GAANNNNTTC 6 cut(s) 2350, 2820, 2991, 3415, 4258, 4290
AspA2I CCTAGG 1 cut(s) 884
AspLEI GCGC 3 cut(s) 2770, 2838, 2980
AsuC2I CCSGG 1 cut(s) 2630
AsuHPI GGTGA 6 cut(s) 1570, 2591, 2858, 2925, 3440, 3785
AvaI CYCGRG 1 cut(s) 669
AvaII GGWCC 7 cut(s) 66, 536, 1333, 1634, 2331, 3114, 3504
AvrII CCTAGG 1 cut(s) 884
AxyI CCTNAGG 2 cut(s) 1587, 1831
BaeGI GKGCMC 3 cut(s) 1264, 2172, 3313
BalI TGGCCA 2 cut(s) 2133, 3085
BamHI GGATCC 1 cut(s) 3851
BanI GGYRCC 1 cut(s) 3074
BanII GRGCYC 1 cut(s) 3139
BarI GAAGNNNNNNTAC 2 cut(s) 1968, 2000
BauI CACGAG 2 cut(s) 2973, 3348
BbsI GAAGAC 4 cut(s) 549, 4023, 4390, 4586
Bbv12I GWGCWC 5 cut(s) 1264, 3130, 3139, 3355, 4156
BceAI ACGGC 8 cut(s) 34, 321, 393, 468, 1900, 2823, 3062, 3739
BcnI CCSGG 1 cut(s) 2630
BfaI CTAG 8 cut(s) 366, 885, 1277, 1320, 2222, 2268, 2502, 3524
BfmI CTRYAG 2 cut(s) 828, 1378
BfoI RGCGCY 1 cut(s) 2839
BfrI CTTAAG 2 cut(s) 1906, 4562
BfuAI ACCTGC 2 cut(s) 3715, 3815
BglI GCCNNNNNGGC 1 cut(s) 4604
BglII AGATCT 3 cut(s) 2090, 2590, 3932
BlnI CCTAGG 1 cut(s) 884
BlpI GCTNAGC 5 cut(s) 1944, 2442, 2637, 3129, 3573
Bme18I GGWCC 7 cut(s) 66, 536, 1333, 1634, 2331, 3114, 3504
BmeRI GACNNNNNGTC 1 cut(s) 4340
BmeT110I CYCGRG 1 cut(s) 669
BmgBI CACGTC 1 cut(s) 509
BmrI ACTGGG 2 cut(s) 172, 192
BmuI ACTGGG 2 cut(s) 172, 192
BpiI GAAGAC 4 cut(s) 549, 4023, 4390, 4586
BplI GAGNNNNNCTC 4 cut(s) 3254, 3286, 4321, 4353
BpmI CTGGAG 6 cut(s) 108, 1851, 2627, 3963, 4140, 4410
Bpu10I CCTNAGC 2 cut(s) 2771, 3354
Bpu1102I GCTNAGC 5 cut(s) 1944, 2442, 2637, 3129, 3573
BpuEI CTTGAG 3 cut(s) 1602, 3597, 4330
BpuMI CCSGG 1 cut(s) 2630
BsaBI GATNNNNATC 4 cut(s) 1293, 2595, 3168, 4201
BsaHI GRCGYC 1 cut(s) 1578
BsaI GGTCTC 2 cut(s) 381, 2868
BsaWI WCCGGW 3 cut(s) 707, 2162, 3146
BsaXI ACNNNNNCTCC 4 cut(s) 2693, 2723, 3936, 3966
Bse1I ACTGG 8 cut(s) 178, 198, 425, 1868, 3032, 3506, 4394, 4448
Bse21I CCTNAGG 2 cut(s) 1587, 1831
Bse3DI GCAATG 4 cut(s) 3230, 3919, 3954, 4629
Bse8I GATNNNNATC 4 cut(s) 1293, 2595, 3168, 4201
BseAI TCCGGA 1 cut(s) 3146
BseJI GATNNNNATC 4 cut(s) 1293, 2595, 3168, 4201
BseMI GCAATG 4 cut(s) 3230, 3919, 3954, 4629
BseNI ACTGG 8 cut(s) 178, 198, 425, 1868, 3032, 3506, 4394, 4448
BseRI GAGGAG 6 cut(s) 26, 1073, 2382, 3212, 4181, 4676
BseSI GKGCMC 3 cut(s) 1264, 2172, 3313
BseX3I CGGCCG 1 cut(s) 1913
BseYI CCCAGC 1 cut(s) 3305
BsgI GTGCAG 2 cut(s) 477, 717
Bsh1285I CGRYCG 2 cut(s) 1916, 4199
BshNI GGYRCC 1 cut(s) 3074
BsiEI CGRYCG 2 cut(s) 1916, 4199
BsiHKAI GWGCWC 5 cut(s) 1264, 3130, 3139, 3355, 4156
BsiHKCI CYCGRG 1 cut(s) 669
BsiSI CCGG 6 cut(s) 708, 1434, 2163, 2629, 3147, 3423
BslFI GGGAC 9 cut(s) 157, 857, 1255, 1565, 1589, 1647, 1699, 2695, 4090
BsmBI CGTCTC 1 cut(s) 4324
BsmFI GGGAC 9 cut(s) 157, 857, 1255, 1565, 1589, 1647, 1699, 2695, 4090
BsmI GAATGC 1 cut(s) 3655
Bso31I GGTCTC 2 cut(s) 381, 2868
BsoBI CYCGRG 1 cut(s) 669
Bsp1286I GDGCHC 7 cut(s) 1264, 2172, 3130, 3139, 3313, 3355, 4156
Bsp13I TCCGGA 1 cut(s) 3146
Bsp1720I GCTNAGC 5 cut(s) 1944, 2442, 2637, 3129, 3573
Bsp19I CCATGG 1 cut(s) 3118
BspEI TCCGGA 1 cut(s) 3146
BspHI TCATGA 1 cut(s) 2107
BspMAI CTGCAG 2 cut(s) 832, 1382
BspMI ACCTGC 2 cut(s) 3715, 3815
BspQI GCTCTTC 3 cut(s) 3645, 3790, 3882
BspT107I GGYRCC 1 cut(s) 3074
BspTI CTTAAG 2 cut(s) 1906, 4562
BspTNI GGTCTC 2 cut(s) 381, 2868
BsrBI CCGCTC 3 cut(s) 3577, 3892, 4682
BsrDI GCAATG 4 cut(s) 3230, 3919, 3954, 4629
BsrI ACTGG 8 cut(s) 178, 198, 425, 1868, 3032, 3506, 4394, 4448
BssNAI GTATAC 1 cut(s) 1114
BssNI GRCGYC 1 cut(s) 1578
BssSI CACGAG 2 cut(s) 2973, 3348
BssT1I CCWWGG 7 cut(s) 625, 790, 884, 914, 2876, 3118, 3379
Bst1107I GTATAC 1 cut(s) 1114
Bst2BI CACGAG 2 cut(s) 2973, 3348
BstACI GRCGYC 1 cut(s) 1578
BstAFI CTTAAG 2 cut(s) 1906, 4562
BstAPI GCANNNNNTGC 2 cut(s) 2318, 4421
BstDSI CCRYGG 2 cut(s) 3118, 4456
BstENI CCTNNNNNAGG 1 cut(s) 2375
BstH2I RGCGCY 1 cut(s) 2839
BstHHI GCGC 3 cut(s) 2770, 2838, 2980
BstMCI CGRYCG 2 cut(s) 1916, 4199
BstNSI RCATGY 5 cut(s) 2246, 2885, 3739, 3769, 4195
BstSFI CTRYAG 2 cut(s) 828, 1378
BstSLI GKGCMC 3 cut(s) 1264, 2172, 3313
BstV2I GAAGAC 4 cut(s) 549, 4023, 4390, 4586
BstX2I RGATCY 7 cut(s) 139, 284, 2090, 2590, 3851, 3932, 4444
BstXI CCANNNNNNTGG 2 cut(s) 626, 3904
BstYI RGATCY 7 cut(s) 139, 284, 2090, 2590, 3851, 3932, 4444
BstZ17I GTATAC 1 cut(s) 1114
BstZI CGGCCG 1 cut(s) 1913
Bsu36I CCTNAGG 2 cut(s) 1587, 1831
BtgI CCRYGG 2 cut(s) 3118, 4456
BtgZI GCGATG 1 cut(s) 4539
BtrI CACGTC 1 cut(s) 509
BtsI GCAGTG 3 cut(s) 452, 1387, 3296
BtsIMutI CAGTG 9 cut(s) 185, 205, 424, 432, 452, 1387, 3039, 3296, 3499
BveI ACCTGC 2 cut(s) 3715, 3815
CaiI CAGNNNCTG 2 cut(s) 650, 2360
CciI TCATGA 1 cut(s) 2107
CfoI GCGC 3 cut(s) 2770, 2838, 2980
Csp6I GTAC 4 cut(s) 486, 1152, 2717, 3769
CviQI GTAC 4 cut(s) 486, 1152, 2717, 3769
DraI TTTAAA 1 cut(s) 4696
DraIII CACNNNGTG 1 cut(s) 509
DriI GACNNNNNGTC 1 cut(s) 4340
EaeI YGGCCR 6 cut(s) 481, 1435, 1913, 2131, 3083, 4249
EagI CGGCCG 1 cut(s) 1913
Eam1105I GACNNNNNGTC 1 cut(s) 4340
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 3137
EclXI CGGCCG 1 cut(s) 1913
Eco130I CCWWGG 7 cut(s) 625, 790, 884, 914, 2876, 3118, 3379
Eco24I GRGCYC 1 cut(s) 3139
Eco31I GGTCTC 2 cut(s) 381, 2868
Eco32I GATATC 1 cut(s) 2044
Eco47I GGWCC 7 cut(s) 66, 536, 1333, 1634, 2331, 3114, 3504
Eco52I CGGCCG 1 cut(s) 1913
Eco53kI GAGCTC 1 cut(s) 3137
Eco57I CTGAAG 7 cut(s) 561, 1029, 1416, 1740, 2568, 2597, 3230
Eco81I CCTNAGG 2 cut(s) 1587, 1831
Eco88I CYCGRG 1 cut(s) 669
EcoICRI GAGCTC 1 cut(s) 3137
EcoNI CCTNNNNNAGG 1 cut(s) 2375
EcoO109I RGGNCCY 3 cut(s) 536, 1333, 3114
EcoRV GATATC 1 cut(s) 2044
EcoT14I CCWWGG 7 cut(s) 625, 790, 884, 914, 2876, 3118, 3379
EcoT22I ATGCAT 4 cut(s) 2329, 2760, 3372, 4500
EcoT38I GRGCYC 1 cut(s) 3139
ErhI CCWWGG 7 cut(s) 625, 790, 884, 914, 2876, 3118, 3379
Esp3I CGTCTC 1 cut(s) 4324
FaqI GGGAC 9 cut(s) 157, 857, 1255, 1565, 1589, 1647, 1699, 2695, 4090
FauI CCCGC 2 cut(s) 3885, 4687
FauNDI CATATG 2 cut(s) 1079, 3870
FblI GTMKAC 3 cut(s) 382, 1113, 1650
FriOI GRGCYC 1 cut(s) 3139
FspBI CTAG 8 cut(s) 366, 885, 1277, 1320, 2222, 2268, 2502, 3524
FspI TGCGCA 1 cut(s) 2979
GlaI GCGC 3 cut(s) 2769, 2837, 2979
GsaI CCCAGC 1 cut(s) 3309
GsuI CTGGAG 6 cut(s) 108, 1851, 2627, 3963, 4140, 4410
HaeII RGCGCY 1 cut(s) 2839
HapII CCGG 6 cut(s) 708, 1434, 2163, 2629, 3147, 3423
HhaI GCGC 3 cut(s) 2770, 2838, 2980
Hin1I GRCGYC 1 cut(s) 1578
Hin6I GCGC 3 cut(s) 2768, 2836, 2978
HinP1I GCGC 3 cut(s) 2768, 2836, 2978
HincII GTYRAC 3 cut(s) 415, 2154, 3212
HindII GTYRAC 3 cut(s) 415, 2154, 3212
HindIII AAGCTT 4 cut(s) 923, 950, 2011, 2690
HpaII CCGG 6 cut(s) 708, 1434, 2163, 2629, 3147, 3423
HphI GGTGA 6 cut(s) 1570, 2591, 2858, 2925, 3440, 3785
Hpy99I CGWCG 3 cut(s) 32, 342, 510
HpyCH4IV ACGT 4 cut(s) 508, 1578, 2784, 2808
HpySE526I ACGT 4 cut(s) 508, 1578, 2784, 2808
Hsp92I GRCGYC 1 cut(s) 1578
HspAI GCGC 3 cut(s) 2768, 2836, 2978
Kpn2I TCCGGA 1 cut(s) 3146
LguI GCTCTTC 3 cut(s) 3645, 3790, 3882
MaeI CTAG 8 cut(s) 366, 885, 1277, 1320, 2222, 2268, 2502, 3524
MaeII ACGT 4 cut(s) 508, 1578, 2784, 2808
MaeIII GTNAC 7 cut(s) 908, 1073, 3027, 3294, 3497, 3678, 4699
MbiI CCGCTC 3 cut(s) 3577, 3892, 4682
MfeI CAATTG 4 cut(s) 2399, 3404, 3489, 4179
MflI RGATCY 7 cut(s) 139, 284, 2090, 2590, 3851, 3932, 4444
MhlI GDGCHC 7 cut(s) 1264, 2172, 3130, 3139, 3313, 3355, 4156
MlsI TGGCCA 2 cut(s) 2133, 3085
MluNI TGGCCA 2 cut(s) 2133, 3085
MlyI GAGTC 9 cut(s) 125, 212, 566, 668, 1853, 3536, 3599, 3722, 3971
MmeI TCCRAC 5 cut(s) 192, 1823, 2168, 2878, 4038
Mox20I TGGCCA 2 cut(s) 2133, 3085
Mph1103I ATGCAT 4 cut(s) 2329, 2760, 3372, 4500
MroI TCCGGA 1 cut(s) 3146
MroXI GAANNNNTTC 6 cut(s) 2350, 2820, 2991, 3415, 4258, 4290
MscI TGGCCA 2 cut(s) 2133, 3085
MseI TTAA 9 cut(s) 606, 1352, 1907, 2016, 3567, 3981, 4188, 4563, 4695
MslI CAYNNNNRTG 8 cut(s) 529, 696, 708, 2517, 3072, 3123, 3483, 3567
Msp20I TGGCCA 2 cut(s) 2133, 3085
MspA1I CMGCKG 5 cut(s) 146, 320, 896, 2488, 3089
MspCI CTTAAG 2 cut(s) 1906, 4562
MspI CCGG 6 cut(s) 708, 1434, 2163, 2629, 3147, 3423
MunI CAATTG 4 cut(s) 2399, 3404, 3489, 4179
Mva1269I GAATGC 1 cut(s) 3655
NciI CCSGG 1 cut(s) 2630
NcoI CCATGG 1 cut(s) 3118
NdeI CATATG 2 cut(s) 1079, 3870
NmeAIII GCCGAG 1 cut(s) 3736
NmuCI GTSAC 3 cut(s) 1073, 3294, 3497
NsbI TGCGCA 1 cut(s) 2979
NsiI ATGCAT 4 cut(s) 2329, 2760, 3372, 4500
NspI RCATGY 5 cut(s) 2246, 2885, 3739, 3769, 4195
OliI CACNNNNGTG 2 cut(s) 708, 3072
PagI TCATGA 1 cut(s) 2107
PciSI GCTCTTC 3 cut(s) 3645, 3790, 3882
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 3075
PctI GAATGC 1 cut(s) 3655
PdmI GAANNNNTTC 6 cut(s) 2350, 2820, 2991, 3415, 4258, 4290
PflMI CCANNNNNTGG 2 cut(s) 3398, 4738
PfoI TCCNGGA 3 cut(s) 62, 1324, 2053
Ple19I CGATCG 1 cut(s) 4199
PleI GAGTC 9 cut(s) 125, 211, 565, 667, 1852, 3536, 3598, 3721, 3970
PpsI GAGTC 9 cut(s) 125, 211, 565, 667, 1852, 3536, 3598, 3721, 3970
PpuMI RGGWCCY 3 cut(s) 536, 1333, 3114
PshBI ATTAAT 2 cut(s) 3567, 3981
Psp124BI GAGCTC 1 cut(s) 3139
Psp1406I AACGTT 1 cut(s) 2808
Psp5II RGGWCCY 3 cut(s) 536, 1333, 3114
PspFI CCCAGC 1 cut(s) 3305
PspPPI RGGWCCY 3 cut(s) 536, 1333, 3114
PsrI GAACNNNNNNTAC 2 cut(s) 625, 657
PstI CTGCAG 2 cut(s) 832, 1382
PstNI CAGNNNCTG 2 cut(s) 650, 2360
PsuI RGATCY 7 cut(s) 139, 284, 2090, 2590, 3851, 3932, 4444
PvuI CGATCG 1 cut(s) 4199
PvuII CAGCTG 2 cut(s) 146, 896
RsaI GTAC 4 cut(s) 487, 1153, 2718, 3770
RsaNI GTAC 4 cut(s) 486, 1152, 2717, 3769
RseI CAYNNNNRTG 8 cut(s) 529, 696, 708, 2517, 3072, 3123, 3483, 3567
SacI GAGCTC 1 cut(s) 3139
SapI GCTCTTC 3 cut(s) 3645, 3790, 3882
SaqAI TTAA 9 cut(s) 606, 1352, 1907, 2016, 3567, 3981, 4188, 4563, 4695
SchI GAGTC 9 cut(s) 125, 212, 566, 668, 1853, 3536, 3599, 3722, 3971
SduI GDGCHC 7 cut(s) 1264, 2172, 3130, 3139, 3313, 3355, 4156
SfcI CTRYAG 2 cut(s) 828, 1378
SinI GGWCC 7 cut(s) 66, 536, 1333, 1634, 2331, 3114, 3504
SmiMI CAYNNNNRTG 8 cut(s) 529, 696, 708, 2517, 3072, 3123, 3483, 3567
SmlI CTYRAG 5 cut(s) 1617, 1906, 3612, 4345, 4562
SmoI CTYRAG 5 cut(s) 1617, 1906, 3612, 4345, 4562
SspI AATATT 2 cut(s) 2494, 4321
SspMI CTAG 8 cut(s) 366, 885, 1277, 1320, 2222, 2268, 2502, 3524
SstI GAGCTC 1 cut(s) 3139
StyI CCWWGG 7 cut(s) 625, 790, 884, 914, 2876, 3118, 3379
TaiI ACGT 4 cut(s) 511, 1581, 2787, 2811
TaqI TCGA 8 cut(s) 18, 562, 2467, 3241, 3619, 3844, 4120, 4134
TaqII GACCGA 2 cut(s) 405, 3521
TatI WGTACW 3 cut(s) 1151, 2716, 3768
TauI GCSGC 7 cut(s) 313, 320, 1915, 3580, 4477, 4607, 4644
Tru1I TTAA 9 cut(s) 606, 1352, 1907, 2016, 3567, 3981, 4188, 4563, 4695
Tru9I TTAA 9 cut(s) 606, 1352, 1907, 2016, 3567, 3981, 4188, 4563, 4695
TscAI CASTG 9 cut(s) 185, 205, 424, 432, 459, 1387, 3039, 3303, 3506
TseFI GTSAC 3 cut(s) 1073, 3294, 3497
Tsp45I GTSAC 3 cut(s) 1073, 3294, 3497
TspGWI ACGGA 3 cut(s) 2171, 3267, 3400
TspRI CASTG 9 cut(s) 185, 205, 424, 432, 459, 1387, 3039, 3303, 3506
Van91I CCANNNNNTGG 2 cut(s) 3398, 4738
Vha464I CTTAAG 2 cut(s) 1906, 4562
VneI GTGCAC 1 cut(s) 1260
VpaK11BI GGWCC 7 cut(s) 66, 536, 1333, 1634, 2331, 3114, 3504
VspI ATTAAT 2 cut(s) 3567, 3981
XagI CCTNNNNNAGG 1 cut(s) 2375
XapI RAATTY 9 cut(s) 718, 775, 1963, 2122, 2350, 2463, 2991, 3603, 4009
XceI RCATGY 5 cut(s) 2246, 2885, 3739, 3769, 4195
XcmI CCANNNNNNNNNTGG 2 cut(s) 626, 2918
XmaJI CCTAGG 1 cut(s) 884
XmiI GTMKAC 3 cut(s) 382, 1113, 1650
XmnI GAANNNNTTC 6 cut(s) 2350, 2820, 2991, 3415, 4258, 4290
XspI CTAG 8 cut(s) 366, 885, 1277, 1320, 2222, 2268, 2502, 3524
ZraI GACGTC 1 cut(s) 1579
Zsp2I ATGCAT 4 cut(s) 2329, 2760, 3372, 4500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.