AT1G27430

GYF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005720
Physical Location & Seq
Reverse (-)
9520825 .. 9527280
6456 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27430.1

Sequence Viewer

Length: 4479 bp
ATGGCCGAAGGGAAGTTCGATCTGCCTGACGATCTCATCTTTTCCAAATCTTCTGATCAGCTCAAAGAATTGGCCTCAGATAACAGCATTCCCTTGTCTCCGCAGTGGCTTTACACGAAATCAAGCGAGTACAAGATGGATGTTCGATCTCCTACTCCTGTTCCCATGGGAAATCCAAGTGACCCAAATCCGAAGGATGCATGGCGCTTGGATGCACCTGAAGACAAAAAGGACTGGAAGAAAATTGTGCATGAGAATGAAACTAGTCGCAGATGGCGTGAAGAGGAAAGAGAAACTGGTTTACTCGGTGCTAGGAAAGTTGATCGACGAAAAACAGAGCGTCGTATTGACAGTGTCTCAAGCAGAGAAACTGGTGACATTAAAAATGCTGCTGCTTCTGATAGGTGGAATGATGTTAATTCTCGTGCTGCTGTACATGAACCCCGGCGCGACAACAAATGGTCATCTCGGTGGGGTCCTGATGACAAAGAGAAAGAAGCTCGTTGTGAGAAGGTAGATATTAACAAGGACAAGGAAGAGCCTCAGAGTGAAAGTCAATCTGTGGTTAGCAATGTCCGTGCAACCTCTGAACGGGACTCTGACACTCGTGACAAATGGAGGCCACGTCATAGGATGGAATCCCAGTCTGGTGGGCCTTCTTCTTATCGCGCTGCACCTGGGTTTGGACTTGATAGAGGACGAGCCGAGGGCCCAAATTTAGGGTTCACTGTGGGCCGAGGAAGGGCATCTACTATTGGGAGGGGTTCTTCAACTTCCTTAATTGGTGCTGGTAGTGCTTTATCCCCTGTGTTTCGTTATCCAAGGGGTAAGTTGCTTGACATGTATAGAAAACAGAAGCCTGATTCTTCCCTAGGTAGGATTTTGACTGAAATGGATGAAGTTGCCTCCATAACGCAAGTGGCTCTGATTGAACCTCTTGCTTTTATTGCACCTGATGCCGAAGAAGAGGCAAACCTTAACGGCATATGGAAGGGAAGGATCATTAGTAGCGAAGTTTACACTTCATCTGGGGAAGAGTCCTTAGGTGGAAACAGTCTGTTGAAATGCCGCATTCCTGAGTCAGGTGAGACAAAAGTGGATGGTGCTTTGCTAGGGTTTATGAATGGTGACAATGGTTCCATGAAAAATAATGATTCCGGATTACTTGGTAGTCATAATGGAGGTCTGGGGGCTGCTTCTAGCGTCCCAAGATTGAATTCAGTGGCTTCTGAAAGTTATGGATCTGGTGGAGCTGGTTATCAGCTTTCTCATGGAAGTCCTGAAGCGGTTAGATCAGTTTTTACTAAGTCCTCTGTGTTGGATGGGAGCGAATCCGTTGTTGGGTCTTTCGAGCAAGCTTATACGGGCAAGCTGCAGCAACCAGATACTGAAGTGGATCACTCAGAAGGGGCTATGCCACCTGAGGAGTTCTTATTCTTGTATATTGATCCTCAAGGAGTAATTCAGGGACCGTTCATTGGTTCTGACATTATTTCATGGTTTGAACAAGGGTTTTTTGGGACAGACCTACAGGTTCGCTTGGCAAGTGCTCCAGAAGGGACTCCTTTTCAAGATCTAGGCAGGGTCATGTCATATATAAAGGCAGAAAGCGTACATGCCCATATCAGTGACCAAAAGAGTGAGCTAGAAGAGACTAGTTTAAAAGCAAATTCAGAGGCTGGTGGATCAGTTGCACATGTAGCAGAATCTAATGATTCATCCTCTTTGACTGGCATATCTCGTTCATTTTCTGTGTATAACAACCCTTCAGGTCAGGATAATTTTCAGAGAAAGTCTGAGTCCGAAGTTTATGGAAGACCACCACATGCCGAGGACCAAAGTTTCTTGGACTTCTCTGCTCAGGATGAAGAAATTGTATTCCCAGGAAGAGCTAGAGTTTCTGGTTATGCATCGTCTGTAAAATCCTCTACAAGTATGCATGATGCTTTAATGGAATTTTCTGGCCACTCTGATATCCCTGTTGAAGTAACTACGGCTGCTACTCGAAATCAGAATGAGAATAAGCTGCACCCATTTGGTGTGTTGTGGTCTGAGCTAGAGGGCGGTAGTACACCAGTTAACCCGTTACCAAACAGATCTTCTGGTGCAATGGGAGAGCCTAGTTGTTCAATAGAAAACCGTCCCATTAATTCCCGAAGAAATTCTCAAATTGACCCCAACATATCCCTCGATGCTTTGTCTGGTAACCGGATGTCACAATTTGAACATGAATCCAACTTCTTTAACCATGGGGATCAGCTTCCATCAAATCAACATCACCAACAGCATTTCCAAAATCGGGATATGCTGTCACATTTACATATAGGTGATCAAGATCTGGAGCATTTAATAACTCTCCAGTTGCAGCAACAACAGAAGATTCAGATGCAACAGCAGCAGAAGATTCAATTGCAACAGCAGCAGAAGATTCAGTTGCAACAGCATCAGTTGGAACAAGAGCATCAGTTACATCAGAAGCTCTTACAGGAGCAGCAACAGTCCCATGCTCGACAATTACATTTTCAACAGATTTTACAAGGACAGACTCCTGATACAAGGTTTGGGCAGTCACATGACTTCCCTCGATCCAACAGTGTTGATCAGATGTTGTTAGAGCAGCAGATGCTGAATGAGCTGCAGAAAAGTTCTGGCCATCCGTCACAAAATTTCGCACCATACATTGAGCAACACGCCGCAGGAAATTTTGGGCGGTTTACACATGAAGGTCACCAAAGGGAGCTACTTGAACAGCTATTTTCAACACAAATGCAATCTCAGTATGGACAAAAGCAATCTCAATATGGACAAATGCAATCTCAACATGGACAACTGCAATCTGAACCAATCCGGTCTTTGGAGTACCAGCTGCTGCAGCAAGAGCAGCTTATGCAATTGGCAAATGGAGTGAGGCACAATACACTATTGGAGGAACAGAGACATATTGACCCTCTGTGGCCATCTGACCATAGTGATCAGCTTTTAAGAACTCATCCTGGGATCCACCGTTCACACTCTTCTGCAGGGTTTAGACCATTAGACTTTCATCAACAGCAGCAGAGGCCACACTTCGAGGATCAGTTTAGTCAACTTGAGCGGAACCGTTCATATCAGCAACAACTTAGACTAGAATTATTGGAGCACGGTCTTCCATTTGAGAGATCAGCATCGGGGCTGAATCTGGATGCAGTAAATGGTCTTGGTCTCTCCCAGGGTCTGGAGTTGCGGGATGCTACTGCCCACATGCAATCTTCTGGCAGATTGGGAAATTCTACTCCGGGTTTCAGTCATCAGAATCCCCGTATACCATTAGGTGAATCACATTTTTCACATTTGGAACCAACGGAAGGACGCTGGTCTGGAGCCGATACACAGCTAGCTGGTGACTGGGCCGAATCTCAATTTCGTAGATCTAATATGGATACTGAGCATGATAAAATGAGGTCGGAAATTAGGAGACTGGGTGAAGATCCCAACTCATGGATGGTAGGTGGATCTACAGACGACAAGTCAAAGCAGCTTTTTATGGAGTTGCTCCACCAGAGACCTGGCCATCAATCTGCTGAGTCACCAAACATGAACCGTGGGTATCCTTATGACAGGATGGTTCCTTCAGGCCTTACCCCAGGGATTCAAACGCTTGGCGGCCTTTCAGACCATGGTGGTAATCAAAACGTTTCATCTGCTTTTGGGGATCGTTCATTTTCTGATGAACAGGTCAACAGAGTACCGGGATATGGGAACAATATGGGATCATTGCACCATAATAGTTCCTTACTTTCTGGAATTATCGATGCTGGGCGGTCAACTCAGAATGAAACTCAAGCCTTCAGTAATATGTTTGGTATGAACAAAGACGCGAATGACATCAATACTTGGAACAATGTGCCGCCTAAAAATGAGGGAATGGGAAGGATGATGTCATATGATGCCCAAGACAGAATGGGCAAGCAAGCAGTATTAGACTCCCTGATTCAAGAGGAGCTTCCTGTTGGTACGCCTGGTCAACAGTCCTCTTTCAATATCTCTGACCGATACAGTGACAACTTGGTTGGAGAAGATAGAAGGAAGGATAGGTTGGTAGTTCCATCACATGGGCAGAATTCAGTTTTGTTAAAAAGGCCTCCCTCATCTCATTCTTCTTCGTCGCATGAAGGATTACTCGAGCGTATGTCTGACACAGCTAGCAGGGCAGCAGCAAGTTCCTATAGTGGAATTGAAGGAGGTGTGAGACGGGAATCAGGAGCAGCAGGAAACAAAGGGTCAACGTCAGAGGCGGCATCGTTTAGTGAGATGTTGAAGAAGAGTAATAGCATGAAGAAGGTGGCGGCAGAGTCAACTGATGCAACCGAAGGAAGCAAAGGTGGCGGTGGTAAGAAGAAAGGGAAGAAAGGGAGGCAAATCGATCCAGCTCTTCTCGGTTTCAAAGTCACTAGCAATCGAATTCTTATGGGCGAGATCCACCGCGCTGATGATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1492

Amino Acids

165.43

Weight (kDa)

5.81

Isoelectric Point (pI)

54.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 480 - 524 4e-11 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 3214, 3477, 4091
AccBSI CCGCTC 1 cut(s) 3094
AccI GTMKAC 1 cut(s) 3301
AccII CGCG 4 cut(s) 450, 669, 3857, 4464
AccIII TCCGGA 1 cut(s) 1157
AclI AACGTT 1 cut(s) 3672
AcoI YGGCCR 5 cut(s) 3, 1963, 2650, 2954, 3547
AcuI CTGAAG 6 cut(s) 240, 1302, 1410, 1752, 3594, 3811
AfaI GTAC 7 cut(s) 131, 435, 1614, 2071, 2861, 3726, 3994
AflIII ACRYGT 2 cut(s) 840, 1696
AhdI GACNNNNNGTC 1 cut(s) 3505
AhlI ACTAGT 2 cut(s) 263, 1655
AjiI CACGTC 1 cut(s) 626
AjnI CCWGG 7 cut(s) 676, 1882, 2992, 3207, 3544, 3622, 3997
AjuI GAANNNNNNNTTGG 8 cut(s) 1201, 1233, 1323, 1355, 2083, 2115, 4058, 4090
AloI GAACNNNNNNTCC 2 cut(s) 2989, 3021
Alw21I GWGCWC 2 cut(s) 1552, 3141
Alw26I GTCTC 9 cut(s) 102, 361, 1082, 1646, 2929, 3206, 3448, 3535, 4222
AlwNI CAGNNNCTG 5 cut(s) 1388, 1679, 2626, 2869, 3214
Ama87I CYCGRG 1 cut(s) 4160
Aor13HI TCCGGA 1 cut(s) 1157
ApaI GGGCCC 1 cut(s) 713
AseI ATTAAT 1 cut(s) 2148
Asp700I GAANNNNTTC 1 cut(s) 2161
AspA2I CCTAGG 1 cut(s) 871
AspLEI GCGC 4 cut(s) 207, 450, 671, 4466
AspS9I GGNCC 8 cut(s) 476, 653, 709, 710, 733, 1469, 1834, 3387
AsuC2I CCSGG 3 cut(s) 445, 3276, 3729
AsuNHI GCTAGC 2 cut(s) 3373, 4181
AvaI CYCGRG 1 cut(s) 4160
AvaII GGWCC 3 cut(s) 476, 1469, 1834
AvrII CCTAGG 1 cut(s) 871
AxyI CCTNAGG 2 cut(s) 1042, 1422
BaeGI GKGCMC 1 cut(s) 713
BaeI ACNNNNGTAYC 2 cut(s) 1377, 1410
BalI TGGCCA 4 cut(s) 1965, 2652, 2956, 3549
BamHI GGATCC 1 cut(s) 2997
BanII GRGCYC 1 cut(s) 713
BarI GAAGNNNNNNTAC 2 cut(s) 733, 765
BauI CACGAG 2 cut(s) 423, 606
BbsI GAAGAC 3 cut(s) 228, 1822, 3137
Bbv12I GWGCWC 2 cut(s) 1552, 3141
BceAI ACGGC 2 cut(s) 997, 2010
BciT130I CCWGG 7 cut(s) 678, 1884, 2994, 3209, 3546, 3624, 3999
BciVI GTATCC 2 cut(s) 3412, 3597
BclI TGATCA 4 cut(s) 55, 2329, 2599, 2971
BcnI CCSGG 3 cut(s) 445, 3276, 3729
BcoDI GTCTC 9 cut(s) 102, 361, 1082, 1646, 2929, 3206, 3448, 3535, 4222
BcuI ACTAGT 2 cut(s) 263, 1655
BfmI CTRYAG 7 cut(s) 1373, 1529, 2636, 2870, 3018, 3495, 4204
BfoI RGCGCY 1 cut(s) 208
BfuI GTATCC 2 cut(s) 3412, 3597
BglII AGATCT 4 cut(s) 1573, 2096, 2335, 3407
BlnI CCTAGG 1 cut(s) 871
Bme18I GGWCC 3 cut(s) 476, 1469, 1834
BmeRI GACNNNNNGTC 1 cut(s) 3505
BmeT110I CYCGRG 1 cut(s) 4160
BmgBI CACGTC 1 cut(s) 626
BmgT120I GGNCC 8 cut(s) 476, 653, 709, 710, 733, 1469, 1834, 3387
BmiI GGNNCC 9 cut(s) 477, 711, 1138, 1470, 2999, 3098, 3336, 3361, 3606
BmrI ACTGGG 3 cut(s) 637, 3394, 3467
BmtI GCTAGC 2 cut(s) 3377, 4185
BmuI ACTGGG 3 cut(s) 637, 3394, 3467
BoxI GACNNNNGTC 1 cut(s) 3352
BpiI GAAGAC 3 cut(s) 228, 1822, 3137
BpmI CTGGAG 5 cut(s) 1536, 2342, 2360, 3236, 3378
Bpu10I CCTNAGC 1 cut(s) 1860
BpuEI CTTGAG 4 cut(s) 343, 1437, 3110, 3804
BpuMI CCSGG 3 cut(s) 445, 3276, 3729
Bsa29I ATCGAT 2 cut(s) 3789, 4401
BsaBI GATNNNNATC 2 cut(s) 2334, 3163
BsaI GGTCTC 2 cut(s) 3206, 3535
BsaWI WCCGGW 3 cut(s) 1157, 2208, 2847
BsaXI ACNNNNNCTCC 4 cut(s) 610, 640, 3445, 3475
Bse1I ACTGG 9 cut(s) 239, 301, 376, 643, 1735, 2075, 2359, 3389, 3462
Bse21I CCTNAGG 2 cut(s) 1042, 1422
Bse3DI GCAATG 3 cut(s) 577, 2115, 3752
Bse8I GATNNNNATC 2 cut(s) 2334, 3163
BseAI TCCGGA 1 cut(s) 1157
BseBI CCWGG 7 cut(s) 678, 1884, 2994, 3209, 3546, 3624, 3999
BseCI ATCGAT 2 cut(s) 3789, 4401
BseJI GATNNNNATC 2 cut(s) 2334, 3163
BseMI GCAATG 3 cut(s) 577, 2115, 3752
BseNI ACTGG 9 cut(s) 239, 301, 376, 643, 1735, 2075, 2359, 3389, 3462
BseRI GAGGAG 2 cut(s) 1439, 3992
BseSI GKGCMC 1 cut(s) 713
BseYI CCCAGC 1 cut(s) 3794
BsgI GTGCAG 2 cut(s) 657, 2012
Bsh1236I CGCG 4 cut(s) 450, 669, 3857, 4464
BshVI ATCGAT 2 cut(s) 3789, 4401
BsiHKAI GWGCWC 2 cut(s) 1552, 3141
BsiHKCI CYCGRG 1 cut(s) 4160
BsiSI CCGG 6 cut(s) 445, 1158, 2209, 2848, 3275, 3728
BslFI GGGAC 7 cut(s) 608, 1190, 1482, 1534, 1573, 2127, 2485
BsmAI GTCTC 9 cut(s) 102, 361, 1082, 1646, 2929, 3206, 3448, 3535, 4222
BsmBI CGTCTC 1 cut(s) 4222
BsmFI GGGAC 7 cut(s) 608, 1190, 1482, 1534, 1573, 2127, 2485
BsmI GAATGC 2 cut(s) 87, 1071
Bso31I GGTCTC 2 cut(s) 3206, 3535
BsoBI CYCGRG 1 cut(s) 4160
Bsp120I GGGCCC 1 cut(s) 709
Bsp1286I GDGCHC 3 cut(s) 713, 1552, 3141
Bsp13I TCCGGA 1 cut(s) 1157
Bsp1407I TGTACA 1 cut(s) 433
Bsp19I CCATGG 3 cut(s) 165, 2248, 3655
BspDI ATCGAT 2 cut(s) 3789, 4401
BspEI TCCGGA 1 cut(s) 1157
BspFNI CGCG 4 cut(s) 450, 669, 3857, 4464
BspLI GGNNCC 9 cut(s) 477, 711, 1138, 1470, 2999, 3098, 3336, 3361, 3606
BspMAI CTGCAG 4 cut(s) 1377, 2640, 2874, 3022
BspOI GCTAGC 2 cut(s) 3377, 4185
BspQI GCTCTTC 3 cut(s) 531, 1882, 4416
BspTNI GGTCTC 2 cut(s) 3206, 3535
BsrBI CCGCTC 1 cut(s) 3094
BsrDI GCAATG 3 cut(s) 577, 2115, 3752
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 9 cut(s) 239, 301, 376, 643, 1735, 2075, 2359, 3389, 3462
BssNAI GTATAC 1 cut(s) 3302
BssSI CACGAG 2 cut(s) 423, 606
BssT1I CCWWGG 5 cut(s) 165, 821, 871, 2248, 3655
Bst1107I GTATAC 1 cut(s) 3302
Bst2BI CACGAG 2 cut(s) 423, 606
Bst2UI CCWGG 7 cut(s) 678, 1884, 2994, 3209, 3546, 3624, 3999
Bst6I CTCTTC 9 cut(s) 276, 531, 960, 1029, 1644, 1882, 3019, 4295, 4416
BstAPI GCANNNNNTGC 3 cut(s) 956, 2623, 2887
BstAUI TGTACA 1 cut(s) 433
BstC8I GCNNGC 6 cut(s) 1356, 1370, 3375, 3947, 3951, 4183
BstDSI CCRYGG 4 cut(s) 165, 2248, 3580, 3655
BstEII GGTNACC 2 cut(s) 2204, 2726
BstENI CCTNNNNNAGG 1 cut(s) 1080
BstFNI CGCG 4 cut(s) 450, 669, 3857, 4464
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 4 cut(s) 207, 450, 671, 4466
BstMAI GTCTC 9 cut(s) 102, 361, 1082, 1646, 2929, 3206, 3448, 3535, 4222
BstNI CCWGG 7 cut(s) 678, 1884, 2994, 3209, 3546, 3624, 3999
BstNSI RCATGY 5 cut(s) 844, 1619, 1700, 1829, 3244
BstPAI GACNNNNGTC 1 cut(s) 3352
BstPI GGTNACC 2 cut(s) 2204, 2726
BstSFI CTRYAG 7 cut(s) 1373, 1529, 2636, 2870, 3018, 3495, 4204
BstSLI GKGCMC 1 cut(s) 713
BstUI CGCG 4 cut(s) 450, 669, 3857, 4464
BstV2I GAAGAC 3 cut(s) 228, 1822, 3137
BstX2I RGATCY 9 cut(s) 1241, 1573, 2096, 2335, 2997, 3407, 3466, 3491, 4455
BstXI CCANNNNNNTGG 2 cut(s) 650, 3545
BstYI RGATCY 9 cut(s) 1241, 1573, 2096, 2335, 2997, 3407, 3466, 3491, 4455
BstZ17I GTATAC 1 cut(s) 3302
Bsu15I ATCGAT 2 cut(s) 3789, 4401
Bsu36I CCTNAGG 2 cut(s) 1042, 1422
BsuI GTATCC 2 cut(s) 3412, 3597
BsuTUI ATCGAT 2 cut(s) 3789, 4401
BtgI CCRYGG 4 cut(s) 165, 2248, 3580, 3655
BtrI CACGTC 1 cut(s) 626
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 7 cut(s) 110, 358, 726, 1227, 1633, 2599, 4042
Cac8I GCNNGC 6 cut(s) 1356, 1370, 3375, 3947, 3951, 4183
CaiI CAGNNNCTG 5 cut(s) 1388, 1679, 2626, 2869, 3214
CfoI GCGC 4 cut(s) 207, 450, 671, 4466
Cfr13I GGNCC 8 cut(s) 476, 653, 709, 710, 733, 1469, 1834, 3387
ClaI ATCGAT 2 cut(s) 3789, 4401
CseI GACGC 4 cut(s) 329, 1192, 3357, 3863
Csp6I GTAC 7 cut(s) 130, 434, 1613, 2070, 2860, 3725, 3993
CviQI GTAC 7 cut(s) 130, 434, 1613, 2070, 2860, 3725, 3993
DraI TTTAAA 1 cut(s) 1662
DriI GACNNNNNGTC 1 cut(s) 3505
EaeI YGGCCR 5 cut(s) 3, 1963, 2650, 2954, 3547
Eam1104I CTCTTC 9 cut(s) 276, 531, 960, 1029, 1644, 1882, 3019, 4295, 4416
Eam1105I GACNNNNNGTC 1 cut(s) 3505
EarI CTCTTC 9 cut(s) 276, 531, 960, 1029, 1644, 1882, 3019, 4295, 4416
Eco130I CCWWGG 5 cut(s) 165, 821, 871, 2248, 3655
Eco147I AGGCCT 2 cut(s) 3615, 4120
Eco24I GRGCYC 1 cut(s) 713
Eco31I GGTCTC 2 cut(s) 3206, 3535
Eco32I GATATC 1 cut(s) 1975
Eco47I GGWCC 3 cut(s) 476, 1469, 1834
Eco57I CTGAAG 6 cut(s) 240, 1302, 1410, 1752, 3594, 3811
Eco81I CCTNAGG 2 cut(s) 1042, 1422
Eco88I CYCGRG 1 cut(s) 4160
Eco91I GGTNACC 2 cut(s) 2204, 2726
EcoNI CCTNNNNNAGG 1 cut(s) 1080
EcoO109I RGGNCCY 2 cut(s) 476, 709
EcoO65I GGTNACC 2 cut(s) 2204, 2726
EcoRI GAATTC 3 cut(s) 1216, 4099, 4440
EcoRII CCWGG 7 cut(s) 676, 1882, 2992, 3207, 3544, 3622, 3997
EcoRV GATATC 1 cut(s) 1975
EcoT14I CCWWGG 5 cut(s) 165, 821, 871, 2248, 3655
EcoT22I ATGCAT 3 cut(s) 202, 1912, 1941
EcoT38I GRGCYC 1 cut(s) 713
ErhI CCWWGG 5 cut(s) 165, 821, 871, 2248, 3655
Esp3I CGTCTC 1 cut(s) 4222
FalI AAGNNNNNCTT 4 cut(s) 2868, 2900, 3966, 3998
FaqI GGGAC 7 cut(s) 608, 1190, 1482, 1534, 1573, 2127, 2485
FauI CCCGC 1 cut(s) 3216
FauNDI CATATG 2 cut(s) 986, 3922
FbaI TGATCA 4 cut(s) 55, 2329, 2599, 2971
FblI GTMKAC 1 cut(s) 3301
FriOI GRGCYC 1 cut(s) 713
GlaI GCGC 4 cut(s) 206, 449, 670, 4465
GsaI CCCAGC 1 cut(s) 3798
GsuI CTGGAG 5 cut(s) 1536, 2342, 2360, 3236, 3378
HaeII RGCGCY 1 cut(s) 208
HapII CCGG 6 cut(s) 445, 1158, 2209, 2848, 3275, 3728
HgaI GACGC 4 cut(s) 329, 1192, 3357, 3863
HhaI GCGC 4 cut(s) 207, 450, 671, 4466
Hin6I GCGC 4 cut(s) 205, 448, 669, 4464
HinP1I GCGC 4 cut(s) 205, 448, 669, 4464
HincII GTYRAC 7 cut(s) 2080, 3086, 3718, 3804, 4004, 4263, 4335
HindII GTYRAC 7 cut(s) 2080, 3086, 3718, 3804, 4004, 4263, 4335
HindIII AAGCTT 1 cut(s) 1356
HpaI GTTAAC 1 cut(s) 2080
HpaII CCGG 6 cut(s) 445, 1158, 2209, 2848, 3275, 3728
Hpy99I CGWCG 3 cut(s) 330, 345, 4147
HpyCH4IV ACGT 3 cut(s) 625, 3672, 4265
HpySE526I ACGT 3 cut(s) 625, 3672, 4265
HspAI GCGC 4 cut(s) 205, 448, 669, 4464
Kpn2I TCCGGA 1 cut(s) 1157
Ksp22I TGATCA 4 cut(s) 55, 2329, 2599, 2971
KspAI GTTAAC 1 cut(s) 2080
LguI GCTCTTC 3 cut(s) 531, 1882, 4416
MaeII ACGT 3 cut(s) 625, 3672, 4265
MbiI CCGCTC 1 cut(s) 3094
MfeI CAATTG 2 cut(s) 2408, 2891
MflI RGATCY 9 cut(s) 1241, 1573, 2096, 2335, 2997, 3407, 3466, 3491, 4455
MhlI GDGCHC 3 cut(s) 713, 1552, 3141
MlsI TGGCCA 4 cut(s) 1965, 2652, 2956, 3549
MluNI TGGCCA 4 cut(s) 1965, 2652, 2956, 3549
MlyI GAGTC 9 cut(s) 590, 1046, 1088, 1555, 1808, 2539, 3572, 3956, 4340
MmeI TCCRAC 6 cut(s) 1299, 2259, 2430, 2613, 3423, 4030
Mox20I TGGCCA 4 cut(s) 1965, 2652, 2956, 3549
Mph1103I ATGCAT 3 cut(s) 202, 1912, 1941
MroI TCCGGA 1 cut(s) 1157
MroXI GAANNNNTTC 1 cut(s) 2161
MscI TGGCCA 4 cut(s) 1965, 2652, 2956, 3549
MslI CAYNNNNRTG 4 cut(s) 255, 469, 1626, 2325
Msp20I TGGCCA 4 cut(s) 1965, 2652, 2956, 3549
MspA1I CMGCKG 1 cut(s) 2866
MspI CCGG 6 cut(s) 445, 1158, 2209, 2848, 3275, 3728
MunI CAATTG 2 cut(s) 2408, 2891
Mva1269I GAATGC 2 cut(s) 87, 1071
MvaI CCWGG 7 cut(s) 678, 1884, 2994, 3209, 3546, 3624, 3999
MvnI CGCG 4 cut(s) 450, 669, 3857, 4464
NciI CCSGG 3 cut(s) 445, 3276, 3729
NcoI CCATGG 3 cut(s) 165, 2248, 3655
NdeI CATATG 2 cut(s) 986, 3922
NheI GCTAGC 2 cut(s) 3373, 4181
NlaIV GGNNCC 9 cut(s) 477, 711, 1138, 1470, 2999, 3098, 3336, 3361, 3606
NmeAIII GCCGAG 3 cut(s) 730, 761, 1855
NsiI ATGCAT 3 cut(s) 202, 1912, 1941
NspI RCATGY 5 cut(s) 844, 1619, 1700, 1829, 3244
PaeR7I CTCGAG 1 cut(s) 4160
PasI CCCWGGG 2 cut(s) 3208, 3623
PceI AGGCCT 2 cut(s) 3615, 4120
PciI ACATGT 2 cut(s) 840, 1696
PciSI GCTCTTC 3 cut(s) 531, 1882, 4416
PcsI WCGNNNNNNNCGW 1 cut(s) 274
PctI GAATGC 2 cut(s) 87, 1071
PdmI GAANNNNTTC 1 cut(s) 2161
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 3 cut(s) 3214, 3477, 4091
PleI GAGTC 9 cut(s) 590, 1045, 1087, 1555, 1807, 2539, 3571, 3956, 4339
PpsI GAGTC 9 cut(s) 590, 1045, 1087, 1555, 1807, 2539, 3571, 3956, 4339
PpuMI RGGWCCY 1 cut(s) 476
PscI ACATGT 2 cut(s) 840, 1696
PshAI GACNNNNGTC 1 cut(s) 3352
PshBI ATTAAT 1 cut(s) 2148
Psp1406I AACGTT 1 cut(s) 3672
Psp5II RGGWCCY 1 cut(s) 476
Psp6I CCWGG 7 cut(s) 676, 1882, 2992, 3207, 3544, 3622, 3997
PspEI GGTNACC 2 cut(s) 2204, 2726
PspFI CCCAGC 1 cut(s) 3794
PspGI CCWGG 7 cut(s) 676, 1882, 2992, 3207, 3544, 3622, 3997
PspN4I GGNNCC 9 cut(s) 477, 711, 1138, 1470, 2999, 3098, 3336, 3361, 3606
PspOMI GGGCCC 1 cut(s) 709
PspPI GGNCC 8 cut(s) 476, 653, 709, 710, 733, 1469, 1834, 3387
PspPPI RGGWCCY 1 cut(s) 476
PspXI VCTCGAGB 1 cut(s) 4160
PstI CTGCAG 4 cut(s) 1377, 2640, 2874, 3022
PstNI CAGNNNCTG 5 cut(s) 1388, 1679, 2626, 2869, 3214
PsuI RGATCY 9 cut(s) 1241, 1573, 2096, 2335, 2997, 3407, 3466, 3491, 4455
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 2866
RsaI GTAC 7 cut(s) 131, 435, 1614, 2071, 2861, 3726, 3994
RsaNI GTAC 7 cut(s) 130, 434, 1613, 2070, 2860, 3725, 3993
RseI CAYNNNNRTG 4 cut(s) 255, 469, 1626, 2325
SapI GCTCTTC 3 cut(s) 531, 1882, 4416
Sau96I GGNCC 8 cut(s) 476, 653, 709, 710, 733, 1469, 1834, 3387
SchI GAGTC 9 cut(s) 590, 1046, 1088, 1555, 1808, 2539, 3572, 3956, 4340
SduI GDGCHC 3 cut(s) 713, 1552, 3141
SfcI CTRYAG 7 cut(s) 1373, 1529, 2636, 2870, 3018, 3495, 4204
Sfr274I CTCGAG 1 cut(s) 4160
SinI GGWCC 3 cut(s) 476, 1469, 1834
SlaI CTCGAG 1 cut(s) 4160
SmiMI CAYNNNNRTG 4 cut(s) 255, 469, 1626, 2325
SmlI CTYRAG 5 cut(s) 358, 1452, 3089, 3819, 4160
SmoI CTYRAG 5 cut(s) 358, 1452, 3089, 3819, 4160
SpeI ACTAGT 2 cut(s) 263, 1655
SseBI AGGCCT 2 cut(s) 3615, 4120
StuI AGGCCT 2 cut(s) 3615, 4120
StyI CCWWGG 5 cut(s) 165, 821, 871, 2248, 3655
TaiI ACGT 3 cut(s) 628, 3675, 4268
TaqII GACCGA 1 cut(s) 4044
TatI WGTACW 3 cut(s) 129, 433, 2069
TauI GCSGC 6 cut(s) 1071, 2696, 3645, 3889, 4277, 4328
TscAI CASTG 7 cut(s) 110, 358, 733, 1227, 1633, 2599, 4042
TspGWI ACGGA 4 cut(s) 566, 1324, 2646, 3356
TspRI CASTG 7 cut(s) 110, 358, 733, 1227, 1633, 2599, 4042
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 3 cut(s) 3214, 3477, 4091
VpaK11BI GGWCC 3 cut(s) 476, 1469, 1834
VspI ATTAAT 1 cut(s) 2148
XagI CCTNNNNNAGG 1 cut(s) 1080
XceI RCATGY 5 cut(s) 844, 1619, 1700, 1829, 3244
XcmI CCANNNNNNNNNTGG 3 cut(s) 916, 3478, 3542
XhoI CTCGAG 1 cut(s) 4160
XmaJI CCTAGG 1 cut(s) 871
XmiI GTMKAC 1 cut(s) 3301
XmnI GAANNNNTTC 1 cut(s) 2161
Zsp2I ATGCAT 3 cut(s) 202, 1912, 1941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.