AT1G25420

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007348
Physical Location & Seq
Forward (+)
8915915 .. 8918267
2353 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G25420.4

Sequence Viewer

Length: 972 bp
ATGTCGCTGCTAAACCAACTCTTCAACCGTGGGATCTTTGGCGCCAAATGCAAGACAAGCTTGAACTTGGCGATAGCTCGAATGAAATTGCTGCAAAACAAGAGAGATATGCAGCTTAAACATATGAAGAAGGAGATAGCTCACTTCTTGCAAGCTGGACAAGAACCCATTGCTCGAATTCGGGTGGAGCATGTGATCAGAGAAATGAATTTATGGGCAGCTTATGAGATATTGGAGCTATTCTGCGAGTTTATACTTGCTCGTGTTCCAATTCTTGAAAGTGAAAAGGAATGCCCGAGAGAGTTGAGAGAGGCTATTGCTAGCATTATCTTTGCTGCTCCAAGGTGCTCTGAAGTACCTGATCTTCTTCAAATAAAGAATCTGTTTGGTACAAAATATGGAAAAGAATTTATCATGGTTGCTTCTGAGCTTCGTCCGGATTCTGGTGTCAATCGTACTATCATTGAGAAGCTTTCTCCTACCAGTCCATCTGGAGCGGCAAGGCTCAAGATGTTAAAGGAAATTGCGCAGGAGTACAGTTTGAATTGGGATTCTTCTGCCACGGAAGCAGAGTTCATGAAGAGCCATGAAGACCTACTGGGTGGAGCTAAGCAAATACATCGTCAAGATGGTATCTCTGAATCTCGACCGTCCCAACAAGGCTACGGTCAGTCTTCGGTTTCTAGGGAAGTTGAAAGTCTGCCTGCAGAGGCCACACAGAGATTCCAAAAGCTTCAAGCTCAAAACCCAGTGAGCAAAAGCATGCCATCATCTAAGCTGACTTCAGCCTTTCAAGCTCCTCCTGATACTAGACGGAATCAGACTGATGTAATGGAGATAGCTCGAGCTGCCCTAGCTAGCGCTGATCGTGCAACAGCAGCTGCTCGTGCTGCTGCGCAACTAGTGAATGTCTCTTATGGGGCTACTACACCCACAGTAGCAGCAGAAGGGAAGCCCTTAAACTTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

323

Amino Acids

35.81

Weight (kDa)

9.1

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 1 - 154 4.4e-57 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 528, 897
AccB1I GGYRCC 1 cut(s) 41
AccBSI CCGCTC 1 cut(s) 497
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 1 cut(s) 497
AclWI GGATC 1 cut(s) 41
AcsI RAATTY 3 cut(s) 177, 208, 407
AcuI CTGAAG 2 cut(s) 372, 768
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 4 cut(s) 357, 391, 457, 536
AfeI AGCGCT 1 cut(s) 862
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 8 cut(s) 25, 64, 278, 371, 544, 695, 737, 794
AhlI ACTAGT 1 cut(s) 901
AloI GAACNNNNNNTCC 2 cut(s) 557, 589
Alw21I GWGCWC 1 cut(s) 350
Alw26I GTCTC 1 cut(s) 916
AlwI GGATC 1 cut(s) 41
AlwNI CAGNNNCTG 1 cut(s) 881
Ama87I CYCGRG 2 cut(s) 295, 843
Aor13HI TCCGGA 1 cut(s) 436
Aor51HI AGCGCT 1 cut(s) 862
AoxI GGCC 1 cut(s) 711
ApoI RAATTY 3 cut(s) 177, 208, 407
ArsI GACNNNNNNTTYG 2 cut(s) 607, 639
AspLEI GCGC 4 cut(s) 44, 529, 863, 898
AsuNHI GCTAGC 2 cut(s) 320, 857
AvaI CYCGRG 2 cut(s) 295, 843
BanI GGYRCC 1 cut(s) 41
BarI GAAGNNNNNNTAC 2 cut(s) 348, 380
BauI CACGAG 2 cut(s) 261, 885
BbsI GAAGAC 2 cut(s) 597, 666
Bbv12I GWGCWC 1 cut(s) 350
BccI CCATC 3 cut(s) 496, 623, 775
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BclI TGATCA 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 916
BcuI ACTAGT 1 cut(s) 901
BfaI CTAG 6 cut(s) 321, 684, 810, 854, 858, 902
BfmI CTRYAG 1 cut(s) 705
BfoI RGCGCY 2 cut(s) 45, 864
BlpI GCTNAGC 1 cut(s) 609
BmeT110I CYCGRG 2 cut(s) 295, 843
BmiI GGNNCC 1 cut(s) 43
BmrI ACTGGG 2 cut(s) 608, 743
BmtI GCTAGC 2 cut(s) 324, 861
BmuI ACTGGG 2 cut(s) 608, 743
BpiI GAAGAC 2 cut(s) 597, 666
BpmI CTGGAG 1 cut(s) 513
Bpu1102I GCTNAGC 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 3 cut(s) 28, 341, 561
BsaWI WCCGGW 1 cut(s) 436
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse1I ACTGG 3 cut(s) 483, 603, 749
Bse3DI GCAATG 1 cut(s) 168
BseAI TCCGGA 1 cut(s) 436
BseDI CCNNGG 3 cut(s) 28, 341, 561
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 3 cut(s) 483, 603, 749
BseRI GAGGAG 1 cut(s) 789
Bsh1285I CGRYCG 1 cut(s) 650
BshFI GGCC 1 cut(s) 713
BshNI GGYRCC 1 cut(s) 41
BsiEI CGRYCG 1 cut(s) 650
BsiHKAI GWGCWC 1 cut(s) 350
BsiHKCI CYCGRG 2 cut(s) 295, 843
BsiSI CCGG 1 cut(s) 437
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 1 cut(s) 916
BsmFI GGGAC 1 cut(s) 637
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 1 cut(s) 713
BsoBI CYCGRG 2 cut(s) 295, 843
Bsp1286I GDGCHC 1 cut(s) 350
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 4 cut(s) 33, 195, 361, 865
Bsp1720I GCTNAGC 1 cut(s) 609
BspACI CCGC 1 cut(s) 497
BspANI GGCC 1 cut(s) 713
BspCNI CTCAG 1 cut(s) 418
BspEI TCCGGA 1 cut(s) 436
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 1 cut(s) 43
BspMAI CTGCAG 1 cut(s) 709
BspOI GCTAGC 2 cut(s) 324, 861
BspPI GGATC 1 cut(s) 41
BspQI GCTCTTC 1 cut(s) 575
BspT107I GGYRCC 1 cut(s) 41
BsrBI CCGCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 3 cut(s) 483, 603, 749
BssECI CCNNGG 3 cut(s) 28, 341, 561
BssMI GATC 4 cut(s) 33, 195, 361, 865
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 2 cut(s) 261, 885
BssT1I CCWWGG 1 cut(s) 341
Bst2BI CACGAG 2 cut(s) 261, 885
Bst4CI ACNGT 5 cut(s) 29, 539, 651, 668, 937
Bst6I CTCTTC 2 cut(s) 26, 575
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 5 cut(s) 153, 322, 705, 764, 859
BstDEI CTNAG 3 cut(s) 426, 609, 774
BstDSI CCRYGG 2 cut(s) 28, 561
BstH2I RGCGCY 2 cut(s) 45, 864
BstHHI GCGC 4 cut(s) 44, 529, 863, 898
BstKTI GATC 4 cut(s) 36, 198, 364, 868
BstMAI GTCTC 1 cut(s) 916
BstMBI GATC 4 cut(s) 33, 195, 361, 865
BstMCI CGRYCG 1 cut(s) 650
BstNSI RCATGY 2 cut(s) 194, 766
BstSFI CTRYAG 1 cut(s) 705
BstV2I GAAGAC 2 cut(s) 597, 666
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BsuRI GGCC 1 cut(s) 713
BtgI CCRYGG 2 cut(s) 28, 561
BtsIMutI CAGTG 1 cut(s) 756
Cac8I GCNNGC 5 cut(s) 153, 322, 705, 764, 859
CaiI CAGNNNCTG 1 cut(s) 881
CciI TCATGA 1 cut(s) 576
CfoI GCGC 4 cut(s) 44, 529, 863, 898
Csp6I GTAC 4 cut(s) 356, 390, 456, 535
CviAII CATG 5 cut(s) 191, 415, 577, 587, 763
CviQI GTAC 4 cut(s) 356, 390, 456, 535
DdeI CTNAG 3 cut(s) 426, 609, 774
DinI GGCGCC 1 cut(s) 43
DpnI GATC 4 cut(s) 35, 197, 363, 867
DpnII GATC 4 cut(s) 33, 195, 361, 865
Eam1104I CTCTTC 2 cut(s) 26, 575
EarI CTCTTC 2 cut(s) 26, 575
Eco130I CCWWGG 1 cut(s) 341
Eco47III AGCGCT 1 cut(s) 862
Eco57I CTGAAG 2 cut(s) 372, 768
Eco88I CYCGRG 2 cut(s) 295, 843
EcoRI GAATTC 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 341
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 341
FaeI CATG 5 cut(s) 194, 418, 580, 590, 766
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FaqI GGGAC 1 cut(s) 637
FatI CATG 5 cut(s) 190, 414, 576, 586, 762
FauNDI CATATG 1 cut(s) 123
FbaI TGATCA 1 cut(s) 195
FspBI CTAG 6 cut(s) 321, 684, 810, 854, 858, 902
FspI TGCGCA 2 cut(s) 528, 897
GlaI GCGC 4 cut(s) 43, 528, 862, 897
GsuI CTGGAG 1 cut(s) 513
HaeII RGCGCY 2 cut(s) 45, 864
HaeIII GGCC 1 cut(s) 713
HapII CCGG 1 cut(s) 437
HhaI GCGC 4 cut(s) 44, 529, 863, 898
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 5 cut(s) 194, 418, 580, 590, 766
Hin6I GCGC 4 cut(s) 42, 527, 861, 896
HinP1I GCGC 4 cut(s) 42, 527, 861, 896
HindIII AAGCTT 3 cut(s) 58, 470, 731
HinfI GANTC 6 cut(s) 379, 440, 551, 641, 723, 817
HpaII CCGG 1 cut(s) 437
Hpy188I TCNGA 5 cut(s) 200, 352, 427, 640, 822
Hpy188III TCNNGA 8 cut(s) 275, 437, 492, 508, 577, 626, 645, 803
HpyAV CCTTC 2 cut(s) 124, 941
HpyCH4III ACNGT 5 cut(s) 29, 539, 651, 668, 937
HpyCH4V TGCA 6 cut(s) 51, 94, 112, 151, 707, 872
HpyF3I CTNAG 3 cut(s) 426, 609, 774
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 5 cut(s) 194, 418, 580, 590, 766
HspAI GCGC 4 cut(s) 42, 527, 861, 896
KasI GGCGCC 1 cut(s) 41
Kpn2I TCCGGA 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 4 cut(s) 33, 195, 361, 865
LguI GCTCTTC 1 cut(s) 575
LmnI GCTCC 6 cut(s) 187, 235, 343, 494, 605, 802
MaeI CTAG 6 cut(s) 321, 684, 810, 854, 858, 902
MalI GATC 4 cut(s) 35, 197, 363, 867
MbiI CCGCTC 1 cut(s) 497
MboI GATC 4 cut(s) 33, 195, 361, 865
MboII GAAGA 8 cut(s) 13, 139, 356, 359, 546, 592, 602, 666
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 1 cut(s) 350
MluCI AATT 7 cut(s) 86, 177, 208, 270, 407, 522, 544
Mly113I GGCGCC 1 cut(s) 42
MnlI CCTC 3 cut(s) 304, 703, 810
MroI TCCGGA 1 cut(s) 436
MseI TTAA 4 cut(s) 117, 515, 959, 965
MspA1I CMGCKG 1 cut(s) 881
MspI CCGG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 296
NarI GGCGCC 1 cut(s) 42
NdeI CATATG 1 cut(s) 123
NdeII GATC 4 cut(s) 33, 195, 361, 865
NheI GCTAGC 2 cut(s) 320, 857
NlaIII CATG 5 cut(s) 194, 418, 580, 590, 766
NlaIV GGNNCC 1 cut(s) 43
NsbI TGCGCA 2 cut(s) 528, 897
NspI RCATGY 2 cut(s) 194, 766
PaeI GCATGC 1 cut(s) 766
PaeR7I CTCGAG 1 cut(s) 843
PagI TCATGA 1 cut(s) 576
PciSI GCTCTTC 1 cut(s) 575
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 6 cut(s) 379, 440, 551, 641, 723, 817
PluTI GGCGCC 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 43
PspXI VCTCGAGB 1 cut(s) 843
PstI CTGCAG 1 cut(s) 709
PstNI CAGNNNCTG 1 cut(s) 881
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 881
RsaI GTAC 4 cut(s) 357, 391, 457, 536
RsaNI GTAC 4 cut(s) 356, 390, 456, 535
SapI GCTCTTC 1 cut(s) 575
SaqAI TTAA 4 cut(s) 117, 515, 959, 965
Sau3AI GATC 4 cut(s) 33, 195, 361, 865
SduI GDGCHC 1 cut(s) 350
SfcI CTRYAG 1 cut(s) 705
SfoI GGCGCC 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 843
SlaI CTCGAG 1 cut(s) 843
SmlI CTYRAG 2 cut(s) 506, 843
SmoI CTYRAG 2 cut(s) 506, 843
SpeI ACTAGT 1 cut(s) 901
SphI GCATGC 1 cut(s) 766
Sse9I AATT 7 cut(s) 86, 177, 208, 270, 407, 522, 544
SsiI CCGC 1 cut(s) 497
SspDI GGCGCC 1 cut(s) 41
SspMI CTAG 6 cut(s) 321, 684, 810, 854, 858, 902
StyI CCWWGG 1 cut(s) 341
TaaI ACNGT 5 cut(s) 29, 539, 651, 668, 937
TaqI TCGA 4 cut(s) 79, 175, 646, 844
TasI AATT 7 cut(s) 86, 177, 208, 270, 407, 522, 544
TatI WGTACW 1 cut(s) 534
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 6 cut(s) 379, 440, 551, 641, 723, 817
Tru1I TTAA 4 cut(s) 117, 515, 959, 965
Tru9I TTAA 4 cut(s) 117, 515, 959, 965
TscAI CASTG 1 cut(s) 756
TspDTI ATGAA 6 cut(s) 98, 140, 221, 565, 593, 603
TspGWI ACGGA 2 cut(s) 578, 829
TspRI CASTG 1 cut(s) 756
XapI RAATTY 3 cut(s) 177, 208, 407
XceI RCATGY 2 cut(s) 194, 766
XhoI CTCGAG 1 cut(s) 843
XspI CTAG 6 cut(s) 321, 684, 810, 854, 858, 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.