RchiOBHm_Chr4g0435061

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007348
Physical Location & Seq
Forward (+)
58672731 .. 58675452
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40350

Sequence Viewer

Length: 942 bp
ATGTCTCTCTTGAACCAGCTCTTCAATAGAGGCGTTTTCGGCACAAAATGCAAAACATGTCTGAACTTGGCAATTTCGCGGATTAAGTTGCTGCAAAACAAGAGAGATATGCAGCTTAAACATATGCGCAAGGAGATTGCTCAGTTTTTGCAGGCTGGCCAAGAAGCAATAGCTCGAATTCGGGTGGAGCATGTTATACGAGAGCAAAACATATGGGCTGCTTATGAGATATTGGAGCTTTTCTGTGAATTTGTCCTCGCAAGGGTTCCCATTATTGAGAGCCAAAAGGATTGCCCACCAGAACTACGAGAGGCTATATCAAGCATAATTTTTTCTGCCCCAAGATGTTCAGAAGTTCCGGATTTATTGCAGATCAAGAATTTGTTTACAGCAAAATATGGGAAGGAATTTATAGCATCTGCCTCTGAGCTTCGTCCTGATTCTGGTGTCAACCGAACTATAATTGAAAAGCTTTCAGTTAGTGCTCCCTCTGGAGAGGCAAGGCTTAAGGTATTGAAGGAAATCGCACAAGAGTACAATCTGAATTGGGATTCTTCTAGCACGGAAGCGGAATTCAGTAGAAAACATGAAGATCTGCTGGGTGGATCCAAGCACATAAGTGGTGGAGCTGCACTGCCTTCTCGAGCTCCTGCAAATCAAGTGTCTTTTAGTTCTCCGCCTTCTAATGGTGCACATTATATTCAGCCCCCAGAGATTAAACCAGAACCTCAACAACACATTCCTGCTCCAAGATCTTTAATTCAGACGCATGTGTCAAGTACGGATGAGATTCAGCCATCAATTAATAATGATGCAGTACCAGTTAGGGAAACAAGACCACAATCATCTGAGATCCTGGAGAAAGCTCGAGCTGCCATTGCGTCTGCTGAACGCGCATCTGCTGCTGCCCGTTCAGCTGCTGAGCTGGTTAAGTCATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

313

Amino Acids

34.61

Weight (kDa)

8.54

Isoelectric Point (pI)

59.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 8.5e-66 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 772
Acc16I TGCGCA 1 cut(s) 128
AccII CGCG 2 cut(s) 79, 894
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 3 cut(s) 79, 569, 677
AclWI GGATC 3 cut(s) 600, 613, 847
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 5 cut(s) 177, 248, 379, 407, 572
AfaI GTAC 3 cut(s) 536, 781, 819
AfiI CCNNNNNNNGG 3 cut(s) 262, 443, 686
AflII CTTAAG 1 cut(s) 506
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 4 cut(s) 13, 25, 467, 517
AjnI CCWGG 1 cut(s) 855
AleI CACNNNNGTG 1 cut(s) 618
Alw21I GWGCWC 3 cut(s) 487, 649, 694
Alw26I GTCTC 1 cut(s) 9
Alw44I GTGCAC 1 cut(s) 690
AlwI GGATC 3 cut(s) 600, 613, 847
AlwNI CAGNNNCTG 1 cut(s) 920
Ama87I CYCGRG 2 cut(s) 642, 867
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 1 cut(s) 157
ApaLI GTGCAC 1 cut(s) 690
ApeKI GCWGC 8 cut(s) 91, 112, 218, 629, 872, 902, 905, 917
ApoI RAATTY 5 cut(s) 177, 248, 379, 407, 572
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
AseI ATTAAT 1 cut(s) 804
AspLEI GCGC 2 cut(s) 129, 896
AvaI CYCGRG 2 cut(s) 642, 867
BaeGI GKGCMC 1 cut(s) 694
BalI TGGCCA 1 cut(s) 159
BamHI GGATCC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 649
Bbv12I GWGCWC 3 cut(s) 487, 649, 694
BbvI GCAGC 8 cut(s) 78, 124, 205, 616, 859, 889, 892, 904
BccI CCATC 1 cut(s) 805
BciT130I CCWGG 1 cut(s) 857
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 558
BfrI CTTAAG 1 cut(s) 506
BglII AGATCT 2 cut(s) 592, 752
BisI GCNGC 8 cut(s) 92, 113, 219, 630, 873, 903, 906, 918
BlpI GCTNAGC 1 cut(s) 921
BlsI GCNGC 8 cut(s) 93, 114, 220, 631, 874, 904, 907, 919
Bme1390I CCNGG 1 cut(s) 857
BmeT110I CYCGRG 2 cut(s) 642, 867
BmiI GGNNCC 2 cut(s) 267, 607
BmrFI CCNGG 1 cut(s) 857
BmsI GCATC 3 cut(s) 425, 802, 905
BpmI CTGGAG 2 cut(s) 513, 878
Bpu1102I GCTNAGC 1 cut(s) 921
BsaWI WCCGGW 1 cut(s) 358
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 443, 686
Bse1I ACTGG 1 cut(s) 821
Bse3DI GCAATG 1 cut(s) 876
BseAI TCCGGA 1 cut(s) 358
BseBI CCWGG 1 cut(s) 857
BseGI GGATG 1 cut(s) 790
BseLI CCNNNNNNNGG 3 cut(s) 262, 443, 686
BseMI GCAATG 1 cut(s) 876
BseMII CTCAG 4 cut(s) 155, 417, 840, 912
BseNI ACTGG 1 cut(s) 821
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 8 cut(s) 78, 124, 205, 616, 859, 889, 892, 904
BseYI CCCAGC 1 cut(s) 598
BsgI GTGCAG 1 cut(s) 615
Bsh1236I CGCG 2 cut(s) 79, 894
BshFI GGCC 1 cut(s) 159
BsiHKAI GWGCWC 3 cut(s) 487, 649, 694
BsiHKCI CYCGRG 2 cut(s) 642, 867
BsiSI CCGG 1 cut(s) 359
BslI CCNNNNNNNGG 3 cut(s) 262, 443, 686
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 159
BsoBI CYCGRG 2 cut(s) 642, 867
Bsp1286I GDGCHC 3 cut(s) 487, 649, 694
Bsp13I TCCGGA 1 cut(s) 358
Bsp143I GATC 5 cut(s) 372, 592, 605, 752, 852
Bsp1720I GCTNAGC 1 cut(s) 921
BspACI CCGC 3 cut(s) 79, 569, 677
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 4 cut(s) 154, 418, 841, 913
BspEI TCCGGA 1 cut(s) 358
BspFNI CGCG 2 cut(s) 79, 894
BspLI GGNNCC 2 cut(s) 267, 607
BspPI GGATC 3 cut(s) 600, 613, 847
BspQI GCTCTTC 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 876
BsrI ACTGG 1 cut(s) 821
BssMI GATC 5 cut(s) 372, 592, 605, 752, 852
Bst2UI CCWGG 1 cut(s) 857
Bst6I CTCTTC 1 cut(s) 26
BstAFI CTTAAG 1 cut(s) 506
BstAPI GCANNNNNTGC 2 cut(s) 48, 902
BstC8I GCNNGC 2 cut(s) 153, 157
BstDEI CTNAG 4 cut(s) 141, 426, 849, 921
BstF5I GGATG 1 cut(s) 790
BstFNI CGCG 2 cut(s) 79, 894
BstHHI GCGC 2 cut(s) 129, 896
BstKTI GATC 5 cut(s) 375, 595, 608, 755, 855
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 5 cut(s) 372, 592, 605, 752, 852
BstMWI GCNNNNNNNGC 7 cut(s) 39, 48, 872, 878, 893, 902, 914
BstNI CCWGG 1 cut(s) 857
BstNSI RCATGY 3 cut(s) 60, 194, 773
BstSCI CCNGG 1 cut(s) 855
BstSLI GKGCMC 1 cut(s) 694
BstUI CGCG 2 cut(s) 79, 894
BstV1I GCAGC 8 cut(s) 78, 124, 205, 616, 859, 889, 892, 904
BstX2I RGATCY 4 cut(s) 592, 605, 752, 852
BstYI RGATCY 4 cut(s) 592, 605, 752, 852
BsuRI GGCC 1 cut(s) 159
BtsCI GGATG 1 cut(s) 790
BtsI GCAGTG 1 cut(s) 632
BtsIMutI CAGTG 1 cut(s) 632
Cac8I GCNNGC 2 cut(s) 153, 157
CaiI CAGNNNCTG 1 cut(s) 920
CfoI GCGC 2 cut(s) 129, 896
CseI GACGC 2 cut(s) 775, 870
Csp6I GTAC 3 cut(s) 535, 780, 818
CviAII CATG 4 cut(s) 57, 191, 587, 770
CviQI GTAC 3 cut(s) 535, 780, 818
DdeI CTNAG 4 cut(s) 141, 426, 849, 921
DpnI GATC 5 cut(s) 374, 594, 607, 754, 854
DpnII GATC 5 cut(s) 372, 592, 605, 752, 852
DrdI GACNNNNNNGTC 1 cut(s) 772
DseDI GACNNNNNNGTC 1 cut(s) 772
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 666
Ecl136II GAGCTC 1 cut(s) 647
Eco24I GRGCYC 1 cut(s) 649
Eco53kI GAGCTC 1 cut(s) 647
Eco88I CYCGRG 2 cut(s) 642, 867
EcoICRI GAGCTC 1 cut(s) 647
EcoRI GAATTC 2 cut(s) 177, 572
EcoRII CCWGG 1 cut(s) 855
EcoT38I GRGCYC 1 cut(s) 649
FaeI CATG 4 cut(s) 60, 194, 590, 773
FatI CATG 4 cut(s) 56, 190, 586, 769
FauNDI CATATG 2 cut(s) 123, 212
Fnu4HI GCNGC 8 cut(s) 92, 113, 219, 630, 873, 903, 906, 918
FokI GGATG 1 cut(s) 797
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 8 cut(s) 92, 113, 219, 630, 873, 903, 906, 918
FspBI CTAG 1 cut(s) 558
FspI TGCGCA 1 cut(s) 128
GlaI GCGC 2 cut(s) 128, 895
GluI GCNGC 8 cut(s) 92, 113, 219, 630, 873, 903, 906, 918
GsaI CCCAGC 1 cut(s) 602
GsuI CTGGAG 2 cut(s) 513, 878
HaeIII GGCC 1 cut(s) 159
HapII CCGG 1 cut(s) 359
HgaI GACGC 2 cut(s) 775, 870
HhaI GCGC 2 cut(s) 129, 896
Hin1II CATG 4 cut(s) 60, 194, 590, 773
Hin6I GCGC 2 cut(s) 127, 894
HinP1I GCGC 2 cut(s) 127, 894
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 3 cut(s) 440, 551, 790
HpaII CCGG 1 cut(s) 359
Hpy166II GTNNAC 3 cut(s) 387, 451, 692
Hpy188I TCNGA 6 cut(s) 63, 352, 427, 543, 765, 850
Hpy188III TCNNGA 6 cut(s) 10, 359, 376, 437, 492, 642
Hpy8I GTNNAC 3 cut(s) 387, 451, 692
HpyAV CCTTC 4 cut(s) 397, 511, 648, 690
HpyCH4V TGCA 9 cut(s) 51, 94, 112, 151, 370, 632, 653, 692, 815
HpyF10VI GCNNNNNNNGC 7 cut(s) 39, 48, 872, 878, 893, 902, 914
HpyF3I CTNAG 4 cut(s) 141, 426, 849, 921
Hsp92II CATG 4 cut(s) 60, 194, 590, 773
HspAI GCGC 2 cut(s) 127, 894
Kpn2I TCCGGA 1 cut(s) 358
Kzo9I GATC 5 cut(s) 372, 592, 605, 752, 852
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 6 cut(s) 187, 235, 490, 626, 652, 751
Lsp1109I GCAGC 8 cut(s) 78, 124, 205, 616, 859, 889, 892, 904
LweI GCATC 3 cut(s) 425, 802, 905
MaeI CTAG 1 cut(s) 558
MalI GATC 5 cut(s) 374, 594, 607, 754, 854
MboI GATC 5 cut(s) 372, 592, 605, 752, 852
MboII GAAGA 3 cut(s) 13, 546, 602
MflI RGATCY 4 cut(s) 592, 605, 752, 852
MhlI GDGCHC 3 cut(s) 487, 649, 694
MlsI TGGCCA 1 cut(s) 159
MluNI TGGCCA 1 cut(s) 159
MnlI CCTC 7 cut(s) 23, 266, 304, 433, 490, 499, 738
Mox20I TGGCCA 1 cut(s) 159
MroI TCCGGA 1 cut(s) 358
MscI TGGCCA 1 cut(s) 159
MseI TTAA 7 cut(s) 84, 117, 507, 717, 758, 804, 930
MslI CAYNNNNRTG 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 917
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 1 cut(s) 857
MvaI CCWGG 1 cut(s) 857
MvnI CGCG 2 cut(s) 79, 894
MwoI GCNNNNNNNGC 7 cut(s) 39, 48, 872, 878, 893, 902, 914
NdeI CATATG 2 cut(s) 123, 212
NdeII GATC 5 cut(s) 372, 592, 605, 752, 852
NlaIII CATG 4 cut(s) 60, 194, 590, 773
NlaIV GGNNCC 2 cut(s) 267, 607
NsbI TGCGCA 1 cut(s) 128
NspI RCATGY 3 cut(s) 60, 194, 773
OliI CACNNNNGTG 1 cut(s) 618
PaeR7I CTCGAG 2 cut(s) 642, 867
PciI ACATGT 1 cut(s) 56
PciSI GCTCTTC 1 cut(s) 26
PfeI GAWTC 3 cut(s) 440, 551, 790
PfoI TCCNGGA 1 cut(s) 855
PkrI GCNGC 8 cut(s) 93, 114, 220, 631, 874, 904, 907, 919
PscI ACATGT 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 649
Psp6I CCWGG 1 cut(s) 855
PspFI CCCAGC 1 cut(s) 598
PspGI CCWGG 1 cut(s) 855
PspN4I GGNNCC 2 cut(s) 267, 607
PspXI VCTCGAGB 1 cut(s) 867
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 4 cut(s) 592, 605, 752, 852
PvuII CAGCTG 1 cut(s) 917
RsaI GTAC 3 cut(s) 536, 781, 819
RsaNI GTAC 3 cut(s) 535, 780, 818
RseI CAYNNNNRTG 1 cut(s) 618
SacI GAGCTC 1 cut(s) 649
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 7 cut(s) 84, 117, 507, 717, 758, 804, 930
SatI GCNGC 8 cut(s) 92, 113, 219, 630, 873, 903, 906, 918
Sau3AI GATC 5 cut(s) 372, 592, 605, 752, 852
ScrFI CCNGG 1 cut(s) 857
SduI GDGCHC 3 cut(s) 487, 649, 694
SfaNI GCATC 3 cut(s) 425, 802, 905
Sfr274I CTCGAG 2 cut(s) 642, 867
SlaI CTCGAG 2 cut(s) 642, 867
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 3 cut(s) 506, 642, 867
SmoI CTYRAG 3 cut(s) 506, 642, 867
SsiI CCGC 3 cut(s) 79, 569, 677
SspMI CTAG 1 cut(s) 558
SstI GAGCTC 1 cut(s) 649
StyD4I CCNGG 1 cut(s) 855
TaqI TCGA 3 cut(s) 175, 643, 868
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 3 cut(s) 440, 551, 790
Tru1I TTAA 7 cut(s) 84, 117, 507, 717, 758, 804, 930
Tru9I TTAA 7 cut(s) 84, 117, 507, 717, 758, 804, 930
TscAI CASTG 1 cut(s) 639
TseI GCWGC 8 cut(s) 91, 112, 218, 629, 872, 902, 905, 917
TspDTI ATGAA 1 cut(s) 603
TspGWI ACGGA 2 cut(s) 578, 797
TspRI CASTG 1 cut(s) 639
Vha464I CTTAAG 1 cut(s) 506
VneI GTGCAC 1 cut(s) 690
VspI ATTAAT 1 cut(s) 804
XapI RAATTY 5 cut(s) 177, 248, 379, 407, 572
XceI RCATGY 3 cut(s) 60, 194, 773
XhoI CTCGAG 2 cut(s) 642, 867
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.