RLG00000006623

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0007348
Physical Location & Seq
Reverse (-)
9124368 .. 9127585
3218 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006623

Sequence Viewer

Length: 1194 bp
ATGACGAAATTGACCTGGTTCTTGCTGTCCGACGACAAGTCGTCGGTGTTGGAAATGGAATTGGGCTCCGAAACGACGTCGTCGCGGTTCTGCTGGGCGTGGATTGTGGAATGGTACTGGGAAAAGATGTCGTCTTCGCCGTCAAAGTCTTCGTCGTCTTCTTCGTGGACGGTGACGAACTGGGCCGGCGGGAAATCAGGATTGGAGGAGATATTACCTGCGAGAGGCTTCATCGTCTTCTTAGAGGCATCCATGTCTCTCTTGAACCAGCTCTTCAATAGAGGCGTTTTCGGCACAAAATGCAAAACATGTCTGAACTTGGCAATTTCGCGGATTAAGTTGCTGCAAAACAAGAGAGATATGCAGCTTAAACATATGCGCAAGGAGATTGCTCAGTTTTTGCAGGCTGGCCAAGAAGCAATAGCTCGAATTCGGGTGGAGCATGTTATACGAGAGCAAAACATATGGGCTGCTTATGAGATACTGGAGCTTTTCTGTGAATTTGTCCTTGCACGGGTTCCCATTATTGAGAGCCAAAAGGATTGCCCACCAGAACTACGAGAGGCTATATCAAGCATAATTTTTTCTGCCCCAAGATGTTCAGAAGTTCCGGATTTATTGCAGATCAAGAATTTGTTTACAGCAAAATATGGGAAGGAATTTATAGCATCTGCCTCTGAGCTTCGTCCTGATTCTGGTGTCAACCGAACTATAATTGAAAAGCTTTTAGTTAGTGCTCCCTCTGGAGAGGCAAGGCTCAAGGTATTGAAGGAAATCGCACAAGAGTACAATCTGAATTGGGATTCTTCTAGCACGGAAGCGGAATTCAGTAGAAAACATGAAGATCTGCTGGGTGGATCCAAGCACATAAGTGGTGGAGCTGCACTGCCTTCTCGAGCTCCGGCAAATCAAGTGTCTTTTAGTTCTCCGCCTTCTAATGGTGCACGTTATATTCAGCCCGCAGAGATTAAACCAGAACCTCAACAACACATTCCTGCTCCAAGATCTTTAATTCAGACGCATGTGTCAAGTACTGATGAGATTCAGCCATCAATTAATAATGATGCAGTACCAGTTAGGGAAACAAGACCACAATCATCTGAGATCCTGGAGAAAGCTCGAGCTGCCATTGCATCTGCTGAACGCGCATCTGCTGCTGCCCGTTCAGCTGCTGAGCTGGTTAAGTCATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

398

Amino Acids

44.09

Weight (kDa)

7.62

Isoelectric Point (pI)

55.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 102 - 265 1.5e-64 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1024
AatII GACGTC 1 cut(s) 80
Acc16I TGCGCA 1 cut(s) 380
Acc36I ACCTGC 1 cut(s) 226
AccII CGCG 3 cut(s) 85, 331, 1146
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 6 cut(s) 85, 189, 331, 821, 929, 960
AclWI GGATC 3 cut(s) 852, 865, 1099
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 5 cut(s) 429, 500, 631, 659, 824
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 4 cut(s) 116, 788, 1033, 1071
AfiI CCNNNNNNNGG 4 cut(s) 224, 514, 695, 938
AflIII ACRYGT 1 cut(s) 308
AgsI TTSAA 4 cut(s) 265, 277, 719, 769
AhdI GACNNNNNGTC 2 cut(s) 37, 40
AjnI CCWGG 2 cut(s) 14, 1107
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AleI CACNNNNGTG 1 cut(s) 870
Alw21I GWGCWC 3 cut(s) 739, 901, 946
Alw26I GTCTC 1 cut(s) 261
Alw44I GTGCAC 1 cut(s) 942
AlwI GGATC 3 cut(s) 852, 865, 1099
AlwNI CAGNNNCTG 1 cut(s) 1172
Ama87I CYCGRG 2 cut(s) 894, 1119
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 2 cut(s) 183, 409
ApaLI GTGCAC 1 cut(s) 942
ApeKI GCWGC 8 cut(s) 343, 364, 470, 881, 1124, 1154, 1157, 1169
ApoI RAATTY 5 cut(s) 429, 500, 631, 659, 824
ArsI GACNNNNNNTTYG 4 cut(s) 137, 169, 899, 931
AseI ATTAAT 1 cut(s) 1056
AspLEI GCGC 2 cut(s) 381, 1148
AspS9I GGNCC 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 184
AvaI CYCGRG 2 cut(s) 894, 1119
BaeGI GKGCMC 1 cut(s) 946
BalI TGGCCA 1 cut(s) 411
BamHI GGATCC 1 cut(s) 857
BanII GRGCYC 2 cut(s) 68, 901
BbsI GAAGAC 4 cut(s) 126, 141, 150, 229
Bbv12I GWGCWC 3 cut(s) 739, 901, 946
BbvI GCAGC 8 cut(s) 330, 376, 457, 868, 1111, 1141, 1144, 1156
BccI CCATC 1 cut(s) 1057
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 2 cut(s) 16, 1109
BcoDI GTCTC 1 cut(s) 261
BfaI CTAG 1 cut(s) 810
BfuAI ACCTGC 1 cut(s) 226
BglII AGATCT 2 cut(s) 844, 1004
BisI GCNGC 8 cut(s) 344, 365, 471, 882, 1125, 1155, 1158, 1170
BlpI GCTNAGC 1 cut(s) 1173
BlsI GCNGC 8 cut(s) 345, 366, 472, 883, 1126, 1156, 1159, 1171
BmcAI AGTACT 1 cut(s) 1033
Bme1390I CCNGG 2 cut(s) 16, 1109
BmeRI GACNNNNNGTC 2 cut(s) 37, 40
BmeT110I CYCGRG 2 cut(s) 894, 1119
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 3 cut(s) 67, 519, 859
BmrFI CCNGG 2 cut(s) 16, 1109
BmrI ACTGGG 2 cut(s) 127, 190
BmsI GCATC 5 cut(s) 257, 677, 1054, 1142, 1157
BmuI ACTGGG 2 cut(s) 127, 190
BpiI GAAGAC 4 cut(s) 126, 141, 150, 229
BpmI CTGGAG 3 cut(s) 506, 765, 1130
Bpu1102I GCTNAGC 1 cut(s) 1173
BpuEI CTTGAG 1 cut(s) 743
BsaHI GRCGYC 1 cut(s) 77
BsaWI WCCGGW 1 cut(s) 610
Bsc4I CCNNNNNNNGG 4 cut(s) 224, 514, 695, 938
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 4 cut(s) 122, 185, 489, 1073
Bse3DI GCAATG 1 cut(s) 1128
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 2 cut(s) 16, 1109
BseGI GGATG 1 cut(s) 248
BseLI CCNNNNNNNGG 4 cut(s) 224, 514, 695, 938
BseMI GCAATG 1 cut(s) 1128
BseMII CTCAG 4 cut(s) 407, 669, 1092, 1164
BseNI ACTGG 4 cut(s) 122, 185, 489, 1073
BseRI GAGGAG 1 cut(s) 221
BseSI GKGCMC 1 cut(s) 946
BseXI GCAGC 8 cut(s) 330, 376, 457, 868, 1111, 1141, 1144, 1156
BseYI CCCAGC 2 cut(s) 93, 850
BsgI GTGCAG 1 cut(s) 867
Bsh1236I CGCG 3 cut(s) 85, 331, 1146
BshFI GGCC 2 cut(s) 185, 411
BsiHKAI GWGCWC 3 cut(s) 739, 901, 946
BsiHKCI CYCGRG 2 cut(s) 894, 1119
BsiSI CCGG 3 cut(s) 186, 611, 902
BslI CCNNNNNNNGG 4 cut(s) 224, 514, 695, 938
BsmAI GTCTC 1 cut(s) 261
BsnI GGCC 2 cut(s) 185, 411
BsoBI CYCGRG 2 cut(s) 894, 1119
Bsp1286I GDGCHC 4 cut(s) 68, 739, 901, 946
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 5 cut(s) 624, 844, 857, 1004, 1104
Bsp1720I GCTNAGC 1 cut(s) 1173
BspACI CCGC 6 cut(s) 85, 189, 331, 821, 929, 960
BspANI GGCC 2 cut(s) 185, 411
BspCNI CTCAG 4 cut(s) 406, 670, 1093, 1165
BspEI TCCGGA 1 cut(s) 610
BspFNI CGCG 3 cut(s) 85, 331, 1146
BspLI GGNNCC 3 cut(s) 67, 519, 859
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 3 cut(s) 852, 865, 1099
BspQI GCTCTTC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 1128
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 4 cut(s) 122, 185, 489, 1073
BssAI RCCGGY 1 cut(s) 185
BssMI GATC 5 cut(s) 624, 844, 857, 1004, 1104
BssNI GRCGYC 1 cut(s) 77
Bst2UI CCWGG 2 cut(s) 16, 1109
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 278
BstACI GRCGYC 1 cut(s) 77
BstAPI GCANNNNNTGC 2 cut(s) 300, 1154
BstC8I GCNNGC 4 cut(s) 187, 405, 409, 960
BstDEI CTNAG 5 cut(s) 241, 393, 678, 1101, 1173
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 248
BstFNI CGCG 3 cut(s) 85, 331, 1146
BstHHI GCGC 2 cut(s) 381, 1148
BstKTI GATC 5 cut(s) 627, 847, 860, 1007, 1107
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 5 cut(s) 624, 844, 857, 1004, 1104
BstMWI GCNNNNNNNGC 7 cut(s) 291, 300, 1124, 1130, 1145, 1154, 1166
BstNI CCWGG 2 cut(s) 16, 1109
BstNSI RCATGY 3 cut(s) 312, 446, 1025
BstSCI CCNGG 2 cut(s) 14, 1107
BstSLI GKGCMC 1 cut(s) 946
BstUI CGCG 3 cut(s) 85, 331, 1146
BstV1I GCAGC 8 cut(s) 330, 376, 457, 868, 1111, 1141, 1144, 1156
BstV2I GAAGAC 4 cut(s) 126, 141, 150, 229
BstX2I RGATCY 4 cut(s) 844, 857, 1004, 1104
BstYI RGATCY 4 cut(s) 844, 857, 1004, 1104
BsuRI GGCC 2 cut(s) 185, 411
BtsCI GGATG 1 cut(s) 248
BtsI GCAGTG 1 cut(s) 884
BtsIMutI CAGTG 1 cut(s) 884
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 4 cut(s) 187, 405, 409, 960
CaiI CAGNNNCTG 1 cut(s) 1172
CfoI GCGC 2 cut(s) 381, 1148
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 183
CseI GACGC 1 cut(s) 1027
CsiI ACCWGGT 1 cut(s) 14
Csp6I GTAC 4 cut(s) 115, 787, 1032, 1070
CviAII CATG 5 cut(s) 253, 309, 443, 839, 1022
CviQI GTAC 4 cut(s) 115, 787, 1032, 1070
DdeI CTNAG 5 cut(s) 241, 393, 678, 1101, 1173
DpnI GATC 5 cut(s) 626, 846, 859, 1006, 1106
DpnII GATC 5 cut(s) 624, 844, 857, 1004, 1104
DrdI GACNNNNNNGTC 1 cut(s) 1024
DriI GACNNNNNGTC 2 cut(s) 37, 40
DseDI GACNNNNNNGTC 1 cut(s) 1024
EaeI YGGCCR 1 cut(s) 409
Eam1104I CTCTTC 1 cut(s) 278
Eam1105I GACNNNNNGTC 2 cut(s) 37, 40
EarI CTCTTC 1 cut(s) 278
EciI GGCGGA 1 cut(s) 918
Ecl136II GAGCTC 1 cut(s) 899
Eco24I GRGCYC 2 cut(s) 68, 901
Eco53kI GAGCTC 1 cut(s) 899
Eco88I CYCGRG 2 cut(s) 894, 1119
EcoICRI GAGCTC 1 cut(s) 899
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoRI GAATTC 2 cut(s) 429, 824
EcoRII CCWGG 2 cut(s) 14, 1107
EcoT38I GRGCYC 2 cut(s) 68, 901
FaeI CATG 5 cut(s) 256, 312, 446, 842, 1025
FatI CATG 5 cut(s) 252, 308, 442, 838, 1021
FauI CCCGC 2 cut(s) 182, 967
FauNDI CATATG 2 cut(s) 375, 464
Fnu4HI GCNGC 8 cut(s) 344, 365, 471, 882, 1125, 1155, 1158, 1170
FokI GGATG 1 cut(s) 235
FriOI GRGCYC 2 cut(s) 68, 901
Fsp4HI GCNGC 8 cut(s) 344, 365, 471, 882, 1125, 1155, 1158, 1170
FspBI CTAG 1 cut(s) 810
FspI TGCGCA 1 cut(s) 380
GlaI GCGC 2 cut(s) 380, 1147
GluI GCNGC 8 cut(s) 344, 365, 471, 882, 1125, 1155, 1158, 1170
GsaI CCCAGC 2 cut(s) 97, 854
GsuI CTGGAG 3 cut(s) 506, 765, 1130
HaeIII GGCC 2 cut(s) 185, 411
HapII CCGG 3 cut(s) 186, 611, 902
HgaI GACGC 1 cut(s) 1027
HhaI GCGC 2 cut(s) 381, 1148
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 5 cut(s) 256, 312, 446, 842, 1025
Hin6I GCGC 2 cut(s) 379, 1146
HinP1I GCGC 2 cut(s) 379, 1146
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HindIII AAGCTT 1 cut(s) 722
HinfI GANTC 3 cut(s) 692, 803, 1042
HpaII CCGG 3 cut(s) 186, 611, 902
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 4 cut(s) 168, 639, 703, 944
Hpy188I TCNGA 8 cut(s) 31, 70, 315, 604, 679, 795, 1017, 1102
Hpy188III TCNNGA 7 cut(s) 198, 262, 611, 628, 689, 744, 894
Hpy8I GTNNAC 4 cut(s) 168, 639, 703, 944
Hpy99I CGWCG 6 cut(s) 35, 46, 79, 82, 85, 157
HpyAV CCTTC 4 cut(s) 649, 763, 900, 942
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 2 cut(s) 77, 946
HpyF10VI GCNNNNNNNGC 7 cut(s) 291, 300, 1124, 1130, 1145, 1154, 1166
HpyF3I CTNAG 5 cut(s) 241, 393, 678, 1101, 1173
HpySE526I ACGT 2 cut(s) 77, 946
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 5 cut(s) 256, 312, 446, 842, 1025
HspAI GCGC 2 cut(s) 379, 1146
Kpn2I TCCGGA 1 cut(s) 610
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Kzo9I GATC 5 cut(s) 624, 844, 857, 1004, 1104
LguI GCTCTTC 1 cut(s) 278
LmnI GCTCC 7 cut(s) 71, 439, 487, 742, 878, 904, 1003
Lsp1109I GCAGC 8 cut(s) 330, 376, 457, 868, 1111, 1141, 1144, 1156
LweI GCATC 5 cut(s) 257, 677, 1054, 1142, 1157
MabI ACCWGGT 1 cut(s) 14
MaeI CTAG 1 cut(s) 810
MaeII ACGT 2 cut(s) 77, 946
MaeIII GTNAC 1 cut(s) 172
MalI GATC 5 cut(s) 626, 846, 859, 1006, 1106
MboI GATC 5 cut(s) 624, 844, 857, 1004, 1104
MboII GAAGA 8 cut(s) 126, 141, 150, 153, 229, 265, 798, 854
MflI RGATCY 4 cut(s) 844, 857, 1004, 1104
MhlI GDGCHC 4 cut(s) 68, 739, 901, 946
MlsI TGGCCA 1 cut(s) 411
MluNI TGGCCA 1 cut(s) 411
MmeI TCCRAC 2 cut(s) 30, 54
MnlI CCTC 9 cut(s) 199, 218, 238, 275, 556, 685, 742, 751, 990
Mox20I TGGCCA 1 cut(s) 411
MroI TCCGGA 1 cut(s) 610
MroNI GCCGGC 1 cut(s) 185
MscI TGGCCA 1 cut(s) 411
MseI TTAA 6 cut(s) 336, 369, 969, 1010, 1056, 1182
MslI CAYNNNNRTG 1 cut(s) 870
Msp20I TGGCCA 1 cut(s) 411
MspA1I CMGCKG 1 cut(s) 1169
MspI CCGG 3 cut(s) 186, 611, 902
MspR9I CCNGG 2 cut(s) 16, 1109
MvaI CCWGG 2 cut(s) 16, 1109
MvnI CGCG 3 cut(s) 85, 331, 1146
MwoI GCNNNNNNNGC 7 cut(s) 291, 300, 1124, 1130, 1145, 1154, 1166
NaeI GCCGGC 1 cut(s) 187
NdeI CATATG 2 cut(s) 375, 464
NdeII GATC 5 cut(s) 624, 844, 857, 1004, 1104
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 5 cut(s) 256, 312, 446, 842, 1025
NlaIV GGNNCC 3 cut(s) 67, 519, 859
NmuCI GTSAC 1 cut(s) 172
NsbI TGCGCA 1 cut(s) 380
NspI RCATGY 3 cut(s) 312, 446, 1025
OliI CACNNNNGTG 1 cut(s) 870
PaeR7I CTCGAG 2 cut(s) 894, 1119
PciI ACATGT 1 cut(s) 308
PciSI GCTCTTC 1 cut(s) 278
PcsI WCGNNNNNNNCGW 2 cut(s) 137, 161
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 3 cut(s) 692, 803, 1042
PflFI GACNNNGTC 1 cut(s) 79
PfoI TCCNGGA 1 cut(s) 1107
PkrI GCNGC 8 cut(s) 345, 366, 472, 883, 1126, 1156, 1159, 1171
PscI ACATGT 1 cut(s) 308
PshBI ATTAAT 1 cut(s) 1056
Psp124BI GAGCTC 1 cut(s) 901
Psp6I CCWGG 2 cut(s) 14, 1107
PspFI CCCAGC 2 cut(s) 93, 850
PspGI CCWGG 2 cut(s) 14, 1107
PspN4I GGNNCC 3 cut(s) 67, 519, 859
PspPI GGNCC 1 cut(s) 183
PspXI VCTCGAGB 1 cut(s) 1119
PstNI CAGNNNCTG 1 cut(s) 1172
PsuI RGATCY 4 cut(s) 844, 857, 1004, 1104
PsyI GACNNNGTC 1 cut(s) 79
PvuII CAGCTG 1 cut(s) 1169
RsaI GTAC 4 cut(s) 116, 788, 1033, 1071
RsaNI GTAC 4 cut(s) 115, 787, 1032, 1070
RseI CAYNNNNRTG 1 cut(s) 870
SacI GAGCTC 1 cut(s) 901
SapI GCTCTTC 1 cut(s) 278
SaqAI TTAA 6 cut(s) 336, 369, 969, 1010, 1056, 1182
SatI GCNGC 8 cut(s) 344, 365, 471, 882, 1125, 1155, 1158, 1170
Sau3AI GATC 5 cut(s) 624, 844, 857, 1004, 1104
Sau96I GGNCC 1 cut(s) 183
ScaI AGTACT 1 cut(s) 1033
ScrFI CCNGG 2 cut(s) 16, 1109
SduI GDGCHC 4 cut(s) 68, 739, 901, 946
SexAI ACCWGGT 1 cut(s) 14
SfaNI GCATC 5 cut(s) 257, 677, 1054, 1142, 1157
Sfr274I CTCGAG 2 cut(s) 894, 1119
SlaI CTCGAG 2 cut(s) 894, 1119
SmiMI CAYNNNNRTG 1 cut(s) 870
SmlI CTYRAG 3 cut(s) 758, 894, 1119
SmoI CTYRAG 3 cut(s) 758, 894, 1119
SsiI CCGC 6 cut(s) 85, 189, 331, 821, 929, 960
SspMI CTAG 1 cut(s) 810
SstI GAGCTC 1 cut(s) 901
StyD4I CCNGG 2 cut(s) 14, 1107
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 2 cut(s) 80, 949
TaqI TCGA 3 cut(s) 427, 895, 1120
TatI WGTACW 2 cut(s) 786, 1031
TfiI GAWTC 3 cut(s) 692, 803, 1042
Tru1I TTAA 6 cut(s) 336, 369, 969, 1010, 1056, 1182
Tru9I TTAA 6 cut(s) 336, 369, 969, 1010, 1056, 1182
TscAI CASTG 1 cut(s) 891
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 8 cut(s) 343, 364, 470, 881, 1124, 1154, 1157, 1169
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 2 cut(s) 220, 855
TspGWI ACGGA 1 cut(s) 830
TspRI CASTG 1 cut(s) 891
Tth111I GACNNNGTC 1 cut(s) 79
VneI GTGCAC 1 cut(s) 942
VspI ATTAAT 1 cut(s) 1056
XagI CCTNNNNNAGG 1 cut(s) 222
XapI RAATTY 5 cut(s) 429, 500, 631, 659, 824
XceI RCATGY 3 cut(s) 312, 446, 1025
XhoI CTCGAG 2 cut(s) 894, 1119
XspI CTAG 1 cut(s) 810
ZraI GACGTC 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.