FvH4_4g27290

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007348
Physical Location & Seq
Forward (+)
28343132 .. 28345909
2778 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27290.t3

Sequence Viewer

Length: 942 bp
ATGTCTCTGTTGAACCAGCTCTTCAATAGAGGCGTTTTCGGCACAAAATGCAAAACATGTTTGCACATGTCGATTTCGCGTATTAAGTTGCTGCAAAACAAGAGAGATATGCAGCTCAAACATATGCGCAAGGAGATTGCTCAATTTTTGCAGGCTGGCCAAGAACCAATAGCTCGAATTCGGGTGGAGCATGTTATACGAGAGCAAAACATATGGGCTGCTTATGAGATATTGGAGCTTTTCTGTGAATTTATCCTTGCACGAGTTCCCATTATTGAAAGCCAAAAGGATTGTCCGCCAGAATTACGAGAGGCTATTTCAAGCATAATATTTTCTGCCCCAAGATGTTCAGAAGTTCCAGATTTATTGCAGATCAAGAATTTATTCACAGCAAAATATGGGAAGGAATTTATAGCAGCTGCATCTGAGCTGCGTCCTGATTCTGGTGTCAACCGAACTATAATTGAAAAGCTGTCGGTGAGTGCTCCCTCTGGAGAGGAAAGGCTCAAGGTATTGAAGGAAATTGCACAAGAGTACAATCTGAATTGGGATTCTTCTAGCTCTGAAGCAGAGTTCAGTAGGAAACATGAAGATCTACTGGGTGGATCCAAGCACATAAGTGGTGGAGCTGCACTGCCTTCTCGAGCTCCTGCAAAACAAGTGTCTTTTAGTCCTCCACCTTCTAATGGTGCACATTATACTCAGCCCACAAAGATTAAACCAGAACCTCAACACATCATTCCAGCTCCAAGATCTTTAATTCAGACACATGTTTCAAGTACCAATGAGATTCAGCCGTCAATTAATAATGATGCAGGACCAGTTAGAGAAACAAGACCACAATCATCTGAGATCCTGGAGAAAGCTCGTGCTGCCATTGCCTCTGCTGAACGTGCATCCGCTGCTGCCCGTTCAGCTGCTGAGCTGGTTAAGTCGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

314

Amino Acids

34.69

Weight (kDa)

9.04

Isoelectric Point (pI)

60.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 1 - 159 4.6e-63 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 128
AccII CGCG 1 cut(s) 79
AciI CCGC 2 cut(s) 296, 900
AclWI GGATC 3 cut(s) 600, 613, 847
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 4 cut(s) 177, 248, 379, 407
AcuI CTGAAG 1 cut(s) 585
AfaI GTAC 2 cut(s) 536, 781
AfiI CCNNNNNNNGG 2 cut(s) 443, 686
AflIII ACRYGT 3 cut(s) 56, 66, 769
AgsI TTSAA 7 cut(s) 13, 25, 278, 321, 467, 517, 777
AjnI CCWGG 1 cut(s) 855
AleI CACNNNNGTG 1 cut(s) 618
Alw21I GWGCWC 3 cut(s) 487, 649, 694
Alw26I GTCTC 1 cut(s) 9
Alw44I GTGCAC 1 cut(s) 690
AlwI GGATC 3 cut(s) 600, 613, 847
AlwNI CAGNNNCTG 1 cut(s) 920
Ama87I CYCGRG 1 cut(s) 642
AoxI GGCC 1 cut(s) 157
ApaLI GTGCAC 1 cut(s) 690
ApoI RAATTY 4 cut(s) 177, 248, 379, 407
ArsI GACNNNNNNTTYG 4 cut(s) 277, 309, 647, 679
AseI ATTAAT 1 cut(s) 804
Asp700I GAANNNNTTC 1 cut(s) 383
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 818
AsuHPI GGTGA 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 642
AvaII GGWCC 1 cut(s) 818
BaeGI GKGCMC 1 cut(s) 694
BalI TGGCCA 1 cut(s) 159
BamHI GGATCC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 649
BauI CACGAG 2 cut(s) 261, 867
Bbv12I GWGCWC 3 cut(s) 487, 649, 694
BceAI ACGGC 1 cut(s) 781
BciT130I CCWGG 1 cut(s) 857
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 558
BglII AGATCT 2 cut(s) 592, 752
BlpI GCTNAGC 1 cut(s) 921
Bme1390I CCNGG 1 cut(s) 857
Bme18I GGWCC 1 cut(s) 818
BmeT110I CYCGRG 1 cut(s) 642
BmgT120I GGNCC 1 cut(s) 818
BmiI GGNNCC 1 cut(s) 607
BmrFI CCNGG 1 cut(s) 857
BmrI ACTGGG 1 cut(s) 608
BmsI GCATC 3 cut(s) 431, 802, 905
BmuI ACTGGG 1 cut(s) 608
BpmI CTGGAG 2 cut(s) 513, 878
Bpu1102I GCTNAGC 1 cut(s) 921
BpuEI CTTGAG 1 cut(s) 491
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 686
Bse1I ACTGG 2 cut(s) 603, 821
Bse3DI GCAATG 1 cut(s) 876
BseBI CCWGG 1 cut(s) 857
BseGI GGATG 1 cut(s) 896
BseLI CCNNNNNNNGG 2 cut(s) 443, 686
BseMI GCAATG 1 cut(s) 876
BseMII CTCAG 4 cut(s) 417, 716, 840, 912
BseNI ACTGG 2 cut(s) 603, 821
BseSI GKGCMC 1 cut(s) 694
BsgI GTGCAG 1 cut(s) 615
Bsh1236I CGCG 1 cut(s) 79
BshFI GGCC 1 cut(s) 159
BsiHKAI GWGCWC 3 cut(s) 487, 649, 694
BsiHKCI CYCGRG 1 cut(s) 642
BslI CCNNNNNNNGG 2 cut(s) 443, 686
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 159
BsoBI CYCGRG 1 cut(s) 642
Bsp1286I GDGCHC 3 cut(s) 487, 649, 694
Bsp143I GATC 5 cut(s) 372, 592, 605, 752, 852
Bsp1720I GCTNAGC 1 cut(s) 921
BspACI CCGC 2 cut(s) 296, 900
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 4 cut(s) 418, 715, 841, 913
BspFNI CGCG 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 607
BspPI GGATC 3 cut(s) 600, 613, 847
BspQI GCTCTTC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 876
BsrI ACTGG 2 cut(s) 603, 821
BssMI GATC 5 cut(s) 372, 592, 605, 752, 852
BssSI CACGAG 2 cut(s) 261, 867
Bst2BI CACGAG 2 cut(s) 261, 867
Bst2UI CCWGG 1 cut(s) 857
Bst6I CTCTTC 1 cut(s) 26
BstAPI GCANNNNNTGC 2 cut(s) 48, 902
BstC8I GCNNGC 2 cut(s) 153, 157
BstDEI CTNAG 4 cut(s) 426, 702, 849, 921
BstF5I GGATG 1 cut(s) 896
BstFNI CGCG 1 cut(s) 79
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 5 cut(s) 375, 595, 608, 755, 855
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 5 cut(s) 372, 592, 605, 752, 852
BstMWI GCNNNNNNNGC 7 cut(s) 39, 48, 872, 878, 893, 902, 914
BstNI CCWGG 1 cut(s) 857
BstNSI RCATGY 4 cut(s) 60, 70, 194, 773
BstSCI CCNGG 1 cut(s) 855
BstSLI GKGCMC 1 cut(s) 694
BstUI CGCG 1 cut(s) 79
BstX2I RGATCY 4 cut(s) 592, 605, 752, 852
BstYI RGATCY 4 cut(s) 592, 605, 752, 852
BsuRI GGCC 1 cut(s) 159
BtsCI GGATG 1 cut(s) 896
BtsI GCAGTG 1 cut(s) 632
BtsIMutI CAGTG 1 cut(s) 632
Cac8I GCNNGC 2 cut(s) 153, 157
CaiI CAGNNNCTG 1 cut(s) 920
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 818
CseI GACGC 1 cut(s) 422
Csp6I GTAC 2 cut(s) 535, 780
CviAII CATG 5 cut(s) 57, 67, 191, 587, 770
CviQI GTAC 2 cut(s) 535, 780
DdeI CTNAG 4 cut(s) 426, 702, 849, 921
DpnI GATC 5 cut(s) 374, 594, 607, 754, 854
DpnII GATC 5 cut(s) 372, 592, 605, 752, 852
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 285
Ecl136II GAGCTC 1 cut(s) 647
Eco24I GRGCYC 1 cut(s) 649
Eco47I GGWCC 1 cut(s) 818
Eco53kI GAGCTC 1 cut(s) 647
Eco57I CTGAAG 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 642
EcoICRI GAGCTC 1 cut(s) 647
EcoRI GAATTC 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 855
EcoT38I GRGCYC 1 cut(s) 649
FaeI CATG 5 cut(s) 60, 70, 194, 590, 773
FatI CATG 5 cut(s) 56, 66, 190, 586, 769
FauNDI CATATG 2 cut(s) 123, 212
FokI GGATG 1 cut(s) 883
FriOI GRGCYC 1 cut(s) 649
FspBI CTAG 1 cut(s) 558
FspI TGCGCA 1 cut(s) 128
GlaI GCGC 1 cut(s) 128
GsuI CTGGAG 2 cut(s) 513, 878
HaeIII GGCC 1 cut(s) 159
HgaI GACGC 1 cut(s) 422
HhaI GCGC 1 cut(s) 129
Hin1II CATG 5 cut(s) 60, 70, 194, 590, 773
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HinfI GANTC 3 cut(s) 440, 551, 790
HphI GGTGA 1 cut(s) 490
Hpy166II GTNNAC 2 cut(s) 451, 692
Hpy188I TCNGA 6 cut(s) 352, 427, 543, 565, 765, 850
Hpy188III TCNNGA 5 cut(s) 359, 376, 437, 492, 642
Hpy8I GTNNAC 2 cut(s) 451, 692
HpyAV CCTTC 4 cut(s) 397, 511, 648, 690
HpyCH4IV ACGT 1 cut(s) 892
HpyF10VI GCNNNNNNNGC 7 cut(s) 39, 48, 872, 878, 893, 902, 914
HpyF3I CTNAG 4 cut(s) 426, 702, 849, 921
HpySE526I ACGT 1 cut(s) 892
Hsp92II CATG 5 cut(s) 60, 70, 194, 590, 773
HspAI GCGC 1 cut(s) 127
Kzo9I GATC 5 cut(s) 372, 592, 605, 752, 852
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 6 cut(s) 187, 235, 490, 626, 652, 751
LweI GCATC 3 cut(s) 431, 802, 905
MaeI CTAG 1 cut(s) 558
MaeII ACGT 1 cut(s) 892
MalI GATC 5 cut(s) 374, 594, 607, 754, 854
MboI GATC 5 cut(s) 372, 592, 605, 752, 852
MboII GAAGA 3 cut(s) 13, 546, 602
MflI RGATCY 4 cut(s) 592, 605, 752, 852
MhlI GDGCHC 3 cut(s) 487, 649, 694
MlsI TGGCCA 1 cut(s) 159
MluNI TGGCCA 1 cut(s) 159
MnlI CCTC 7 cut(s) 23, 304, 490, 499, 684, 738, 892
Mox20I TGGCCA 1 cut(s) 159
MroXI GAANNNNTTC 1 cut(s) 383
MscI TGGCCA 1 cut(s) 159
MseI TTAA 5 cut(s) 84, 717, 758, 804, 930
MslI CAYNNNNRTG 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 3 cut(s) 419, 902, 917
MspR9I CCNGG 1 cut(s) 857
MvaI CCWGG 1 cut(s) 857
MvnI CGCG 1 cut(s) 79
MwoI GCNNNNNNNGC 7 cut(s) 39, 48, 872, 878, 893, 902, 914
NdeI CATATG 2 cut(s) 123, 212
NdeII GATC 5 cut(s) 372, 592, 605, 752, 852
NlaIII CATG 5 cut(s) 60, 70, 194, 590, 773
NlaIV GGNNCC 1 cut(s) 607
NsbI TGCGCA 1 cut(s) 128
NspI RCATGY 4 cut(s) 60, 70, 194, 773
OliI CACNNNNGTG 1 cut(s) 618
PaeR7I CTCGAG 1 cut(s) 642
PciI ACATGT 3 cut(s) 56, 66, 769
PciSI GCTCTTC 1 cut(s) 26
PdmI GAANNNNTTC 1 cut(s) 383
PfeI GAWTC 3 cut(s) 440, 551, 790
PfoI TCCNGGA 1 cut(s) 855
PscI ACATGT 3 cut(s) 56, 66, 769
PshBI ATTAAT 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 649
Psp6I CCWGG 1 cut(s) 855
PspGI CCWGG 1 cut(s) 855
PspN4I GGNNCC 1 cut(s) 607
PspPI GGNCC 1 cut(s) 818
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 4 cut(s) 592, 605, 752, 852
PvuII CAGCTG 2 cut(s) 419, 917
RsaI GTAC 2 cut(s) 536, 781
RsaNI GTAC 2 cut(s) 535, 780
RseI CAYNNNNRTG 1 cut(s) 618
SacI GAGCTC 1 cut(s) 649
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 5 cut(s) 84, 717, 758, 804, 930
Sau3AI GATC 5 cut(s) 372, 592, 605, 752, 852
Sau96I GGNCC 1 cut(s) 818
ScrFI CCNGG 1 cut(s) 857
SduI GDGCHC 3 cut(s) 487, 649, 694
SfaNI GCATC 3 cut(s) 431, 802, 905
Sfr274I CTCGAG 1 cut(s) 642
SinI GGWCC 1 cut(s) 818
SlaI CTCGAG 1 cut(s) 642
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 2 cut(s) 506, 642
SmoI CTYRAG 2 cut(s) 506, 642
SsiI CCGC 2 cut(s) 296, 900
SspI AATATT 1 cut(s) 330
SspMI CTAG 1 cut(s) 558
SstI GAGCTC 1 cut(s) 649
StyD4I CCNGG 1 cut(s) 855
TaiI ACGT 1 cut(s) 895
TaqI TCGA 3 cut(s) 71, 175, 643
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 3 cut(s) 440, 551, 790
Tru1I TTAA 5 cut(s) 84, 717, 758, 804, 930
Tru9I TTAA 5 cut(s) 84, 717, 758, 804, 930
TscAI CASTG 1 cut(s) 639
TspDTI ATGAA 1 cut(s) 603
TspRI CASTG 1 cut(s) 639
VneI GTGCAC 1 cut(s) 690
VpaK11BI GGWCC 1 cut(s) 818
VspI ATTAAT 1 cut(s) 804
XapI RAATTY 4 cut(s) 177, 248, 379, 407
XceI RCATGY 4 cut(s) 60, 70, 194, 773
XhoI CTCGAG 1 cut(s) 642
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.