MD13G1040800.v1.1

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007348
Physical Location & Seq
Forward (+)
2796594 .. 2799512
2919 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1040800.v1.1.491

Sequence Viewer

Length: 948 bp
ATGTCTCTGTTAAACCAGCTCTTCAACCGAGGCGTTTTCGGCACAAAATGCAAAACATGTTTGAATTTGGGTATTTCACGCATCAAATTACTGCAAAACAAGAGAGATGTGCAACTCAAAAATATGCGCAAGGAGATTGCACAGTTTTTGCAGGCTGGCCAAGAAGCAATAGCCCGAATTCGGGTGGAGCATGTTATACGAGAGCAAAACATTTGGGCTGCTTATGAGATATTGGAGCTGTTCTGTGAATTCGTCCTTGCACGGGTTCCCATTATTGAAAGTCAGAGGGAGTGCCCAACGGAATTACGAGAAGCTATATCGAGCATAATTTTTGCTGCTCCAAGATGCTCAGATGTTCCAGATTTAATGCAGATCACAAATTTGTTTACAGCAAAATATGGGAAGGAATTTGTATCAGCTGTGTCTGAGCTTCGTCCTGATTCCGGTGTCAACAGAACAATAATTGAAAAGCTTTCAGTTAGTGCTCCCTCCGGAGAGGCAAGGCTTAAGGTATTGAAGGAAATTGCAAAAGAGTACAAGCTGAATTGGGATTCTTCTGACACTGAAGCAGAGTTTAGTAAAAAGCATGAAGATCTGCTGGGTGGACCGAAGCACATAAGTGGTGCTGCTGCACCTTCTCAAGCTCCCGCAAAACATATCACATTTAGTTCCCCGCCTGCTAATGGGGCACATTCTATTGTGCCTGCAGATATTAAACAAGAACCTCAATACCTTCATGCTCCAAGATCTTTAAGCAAGACACCATCGTTCGCTAATGAGATTCAACCATCAGCGAATGATGATAAAGCAAGACCAGTTAATGAAACCAAAGGGGAGACAAGACCACAATCGTCTGATATCCTCGAGAGAGCTCGAGCTGCCATTGCCTCTGCTGAGCGTGCATCTGCTGCTGCCCGTCAGGCTGCAGAGCTGGTTAAGTCCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

316

Amino Acids

34.75

Weight (kDa)

8.88

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 4.6e-65 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 128
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 2 cut(s) 648, 674
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 5 cut(s) 64, 177, 248, 379, 407
AcuI CTGAAG 1 cut(s) 585
AfaI GTAC 1 cut(s) 536
AfiI CCNNNNNNNGG 5 cut(s) 180, 181, 262, 443, 683
AflII CTTAAG 1 cut(s) 506
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 6 cut(s) 25, 64, 278, 467, 517, 785
AleI CACNNNNGTG 1 cut(s) 618
Alw21I GWGCWC 2 cut(s) 487, 874
Alw26I GTCTC 2 cut(s) 9, 830
Ama87I CYCGRG 2 cut(s) 863, 873
Aor13HI TCCGGA 1 cut(s) 491
AoxI GGCC 1 cut(s) 157
ApeKI GCWGC 8 cut(s) 218, 335, 626, 629, 878, 908, 911, 923
ApoI RAATTY 5 cut(s) 64, 177, 248, 379, 407
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 605
AvaI CYCGRG 2 cut(s) 863, 873
AvaII GGWCC 1 cut(s) 605
BaeGI GKGCMC 2 cut(s) 296, 691
BalI TGGCCA 1 cut(s) 159
BanII GRGCYC 1 cut(s) 874
Bbv12I GWGCWC 2 cut(s) 487, 874
BbvI GCAGC 8 cut(s) 205, 322, 613, 616, 865, 895, 898, 910
BccI CCATC 2 cut(s) 772, 796
BcoDI GTCTC 2 cut(s) 9, 830
BfmI CTRYAG 2 cut(s) 705, 924
BfrI CTTAAG 1 cut(s) 506
BglI GCCNNNNNGGC 1 cut(s) 920
BglII AGATCT 2 cut(s) 592, 746
BisI GCNGC 8 cut(s) 219, 336, 627, 630, 879, 909, 912, 924
BlpI GCTNAGC 1 cut(s) 894
BlsI GCNGC 8 cut(s) 220, 337, 628, 631, 880, 910, 913, 925
Bme18I GGWCC 1 cut(s) 605
BmeT110I CYCGRG 2 cut(s) 863, 873
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 1 cut(s) 267
BmsI GCATC 3 cut(s) 90, 335, 911
Bpu1102I GCTNAGC 1 cut(s) 894
BpuEI CTTGAG 1 cut(s) 624
BsaJI CCNNGG 1 cut(s) 28
BsaWI WCCGGW 2 cut(s) 443, 491
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 181, 262, 443, 683
Bse1I ACTGG 1 cut(s) 815
Bse3DI GCAATG 1 cut(s) 882
BseAI TCCGGA 1 cut(s) 491
BseDI CCNNGG 1 cut(s) 28
BseLI CCNNNNNNNGG 5 cut(s) 180, 181, 262, 443, 683
BseMI GCAATG 1 cut(s) 882
BseMII CTCAG 3 cut(s) 363, 417, 885
BseNI ACTGG 1 cut(s) 815
BseSI GKGCMC 2 cut(s) 296, 691
BseXI GCAGC 8 cut(s) 205, 322, 613, 616, 865, 895, 898, 910
BseYI CCCAGC 1 cut(s) 598
BsgI GTGCAG 1 cut(s) 615
BshFI GGCC 1 cut(s) 159
BsiHKAI GWGCWC 2 cut(s) 487, 874
BsiHKCI CYCGRG 2 cut(s) 863, 873
BsiSI CCGG 2 cut(s) 444, 492
BslI CCNNNNNNNGG 5 cut(s) 180, 181, 262, 443, 683
BsmAI GTCTC 2 cut(s) 9, 830
BsnI GGCC 1 cut(s) 159
BsoBI CYCGRG 2 cut(s) 863, 873
Bsp1286I GDGCHC 4 cut(s) 296, 487, 691, 874
Bsp13I TCCGGA 1 cut(s) 491
Bsp143I GATC 3 cut(s) 372, 592, 746
Bsp1720I GCTNAGC 1 cut(s) 894
BspACI CCGC 2 cut(s) 648, 674
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 3 cut(s) 362, 418, 886
BspEI TCCGGA 1 cut(s) 491
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 2 cut(s) 709, 928
BspQI GCTCTTC 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 882
BsrI ACTGG 1 cut(s) 815
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 3 cut(s) 372, 592, 746
Bst4CI ACNGT 1 cut(s) 144
Bst6I CTCTTC 1 cut(s) 26
BstAFI CTTAAG 1 cut(s) 506
BstAPI GCANNNNNTGC 2 cut(s) 48, 908
BstC8I GCNNGC 5 cut(s) 153, 157, 678, 705, 900
BstDEI CTNAG 3 cut(s) 349, 426, 894
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 3 cut(s) 375, 595, 749
BstMAI GTCTC 2 cut(s) 9, 830
BstMBI GATC 3 cut(s) 372, 592, 746
BstMWI GCNNNNNNNGC 8 cut(s) 39, 48, 686, 878, 884, 899, 908, 920
BstNSI RCATGY 2 cut(s) 60, 194
BstSFI CTRYAG 2 cut(s) 705, 924
BstSLI GKGCMC 2 cut(s) 296, 691
BstV1I GCAGC 8 cut(s) 205, 322, 613, 616, 865, 895, 898, 910
BstX2I RGATCY 2 cut(s) 592, 746
BstYI RGATCY 2 cut(s) 592, 746
BsuRI GGCC 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 561
Cac8I GCNNGC 5 cut(s) 153, 157, 678, 705, 900
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 605
Csp6I GTAC 1 cut(s) 535
CviAII CATG 4 cut(s) 57, 191, 587, 737
CviQI GTAC 1 cut(s) 535
DdeI CTNAG 3 cut(s) 349, 426, 894
DpnI GATC 3 cut(s) 374, 594, 748
DpnII GATC 3 cut(s) 372, 592, 746
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 872
Eco24I GRGCYC 1 cut(s) 874
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 1 cut(s) 605
Eco53kI GAGCTC 1 cut(s) 872
Eco57I CTGAAG 1 cut(s) 585
Eco88I CYCGRG 2 cut(s) 863, 873
EcoICRI GAGCTC 1 cut(s) 872
EcoRI GAATTC 2 cut(s) 177, 248
EcoRV GATATC 1 cut(s) 859
EcoT38I GRGCYC 1 cut(s) 874
FaeI CATG 4 cut(s) 60, 194, 590, 740
FatI CATG 4 cut(s) 56, 190, 586, 736
FauI CCCGC 2 cut(s) 655, 681
Fnu4HI GCNGC 8 cut(s) 219, 336, 627, 630, 879, 909, 912, 924
FriOI GRGCYC 1 cut(s) 874
Fsp4HI GCNGC 8 cut(s) 219, 336, 627, 630, 879, 909, 912, 924
FspI TGCGCA 1 cut(s) 128
GlaI GCGC 1 cut(s) 128
GluI GCNGC 8 cut(s) 219, 336, 627, 630, 879, 909, 912, 924
GsaI CCCAGC 1 cut(s) 602
HaeIII GGCC 1 cut(s) 159
HapII CCGG 2 cut(s) 444, 492
HhaI GCGC 1 cut(s) 129
Hin1II CATG 4 cut(s) 60, 194, 590, 740
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 3 cut(s) 440, 551, 781
HpaII CCGG 2 cut(s) 444, 492
Hpy166II GTNNAC 3 cut(s) 387, 451, 605
Hpy188I TCNGA 5 cut(s) 285, 352, 427, 559, 856
Hpy188III TCNNGA 4 cut(s) 359, 437, 492, 865
Hpy8I GTNNAC 3 cut(s) 387, 451, 605
HpyAV CCTTC 4 cut(s) 397, 511, 645, 743
HpyCH4III ACNGT 1 cut(s) 144
HpyF10VI GCNNNNNNNGC 8 cut(s) 39, 48, 686, 878, 884, 899, 908, 920
HpyF3I CTNAG 3 cut(s) 349, 426, 894
Hsp92II CATG 4 cut(s) 60, 194, 590, 740
HspAI GCGC 1 cut(s) 127
Kpn2I TCCGGA 1 cut(s) 491
Kzo9I GATC 3 cut(s) 372, 592, 746
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 6 cut(s) 187, 235, 343, 490, 649, 745
Lsp1109I GCAGC 8 cut(s) 205, 322, 613, 616, 865, 895, 898, 910
LweI GCATC 3 cut(s) 90, 335, 911
MalI GATC 3 cut(s) 374, 594, 748
MboI GATC 3 cut(s) 372, 592, 746
MboII GAAGA 3 cut(s) 13, 546, 602
MflI RGATCY 2 cut(s) 592, 746
MhlI GDGCHC 4 cut(s) 296, 487, 691, 874
MlsI TGGCCA 1 cut(s) 159
MluNI TGGCCA 1 cut(s) 159
MnlI CCTC 7 cut(s) 23, 279, 490, 499, 735, 872, 898
Mox20I TGGCCA 1 cut(s) 159
MroI TCCGGA 1 cut(s) 491
MscI TGGCCA 1 cut(s) 159
MseI TTAA 7 cut(s) 11, 365, 507, 714, 752, 819, 936
MslI CAYNNNNRTG 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 419
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 2 cut(s) 444, 492
MwoI GCNNNNNNNGC 8 cut(s) 39, 48, 686, 878, 884, 899, 908, 920
NdeII GATC 3 cut(s) 372, 592, 746
NlaIII CATG 4 cut(s) 60, 194, 590, 740
NlaIV GGNNCC 1 cut(s) 267
NsbI TGCGCA 1 cut(s) 128
NspI RCATGY 2 cut(s) 60, 194
OliI CACNNNNGTG 1 cut(s) 618
PaeR7I CTCGAG 2 cut(s) 863, 873
PciI ACATGT 1 cut(s) 56
PciSI GCTCTTC 1 cut(s) 26
PfeI GAWTC 3 cut(s) 440, 551, 781
PkrI GCNGC 8 cut(s) 220, 337, 628, 631, 880, 910, 913, 925
PscI ACATGT 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 874
PspFI CCCAGC 1 cut(s) 598
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 1 cut(s) 605
PspXI VCTCGAGB 1 cut(s) 873
PstI CTGCAG 2 cut(s) 709, 928
PsuI RGATCY 2 cut(s) 592, 746
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
RseI CAYNNNNRTG 1 cut(s) 618
SacI GAGCTC 1 cut(s) 874
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 7 cut(s) 11, 365, 507, 714, 752, 819, 936
SatI GCNGC 8 cut(s) 219, 336, 627, 630, 879, 909, 912, 924
Sau3AI GATC 3 cut(s) 372, 592, 746
Sau96I GGNCC 1 cut(s) 605
SduI GDGCHC 4 cut(s) 296, 487, 691, 874
SfaNI GCATC 3 cut(s) 90, 335, 911
SfcI CTRYAG 2 cut(s) 705, 924
Sfr274I CTCGAG 2 cut(s) 863, 873
SinI GGWCC 1 cut(s) 605
SlaI CTCGAG 2 cut(s) 863, 873
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 4 cut(s) 506, 639, 863, 873
SmoI CTYRAG 4 cut(s) 506, 639, 863, 873
SsiI CCGC 2 cut(s) 648, 674
SstI GAGCTC 1 cut(s) 874
TaaI ACNGT 1 cut(s) 144
TaqI TCGA 3 cut(s) 320, 864, 874
TaqII GACCGA 1 cut(s) 622
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 3 cut(s) 440, 551, 781
Tru1I TTAA 7 cut(s) 11, 365, 507, 714, 752, 819, 936
Tru9I TTAA 7 cut(s) 11, 365, 507, 714, 752, 819, 936
TscAI CASTG 1 cut(s) 568
TseI GCWGC 8 cut(s) 218, 335, 626, 629, 878, 908, 911, 923
TspDTI ATGAA 3 cut(s) 603, 725, 837
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 1 cut(s) 568
Vha464I CTTAAG 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 5 cut(s) 64, 177, 248, 379, 407
XceI RCATGY 2 cut(s) 60, 194
XhoI CTCGAG 2 cut(s) 863, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.