Rorug04G0282200

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007348
Physical Location & Seq
Forward (+)
45561539 .. 45562976
1438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0282200.1

Sequence Viewer

Length: 501 bp
ATGAGGATAGATGATTATGAAGATGAGGATGATGATGATGATGCTCTTGTGGCCTTGAAAGATTGCAAGTATGTATGGACTCAATCACTCAAAGATCTACTTGTCCAAGCAATGGAAAAGCCAATGAGTCTGTGGATTTGGTGTCGTCCATGTCGTTCGGAGGAGACCACTCGCAGATTAGTCGTGACCATACTGGACGTGGATATTGAATCATTTGAGGAGAAGTTGTGGAGGAACAACTTCGGCAGCTCAAGTCCATGCAGTATGGCATGCCTGGCTCTTCAAAATTTTATCAGGCTAAAGAAGCTGGATCCGAGTATGGCAGATTCACCCAGGAAAGAAATGAATTATGCTGGATCATGGGCATATGCTCCTCCGTCATCAACAGTTTCTGATTGCAGACCAAAAGGGAAAGCAATTCAGATAGAAGATAGTGTTGTTGAACACCAACCATCTTTTGATAGTTGGGATCCCATGTACATTAGGGGCGGTGATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

166

Amino Acids

19.1

Weight (kDa)

4.58

Isoelectric Point (pI)

61.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 112
AciI CCGC 1 cut(s) 489
AclWI GGATC 5 cut(s) 305, 318, 364, 464, 477
AcsI RAATTY 1 cut(s) 286
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 1 cut(s) 112
AgsI TTSAA 4 cut(s) 58, 209, 284, 443
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 2 cut(s) 273, 332
AluBI AGCT 2 cut(s) 249, 307
AluI AGCT 2 cut(s) 249, 307
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 5 cut(s) 305, 318, 364, 464, 477
AlwNI CAGNNNCTG 1 cut(s) 392
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 246
ApoI RAATTY 1 cut(s) 286
Asp700I GAANNNNTTC 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 321
BamHI GGATCC 2 cut(s) 310, 469
BbvI GCAGC 1 cut(s) 258
BccI CCATC 1 cut(s) 460
BcgI CGANNNNNNTGC 2 cut(s) 163, 197
BciT130I CCWGG 2 cut(s) 275, 334
BcoDI GTCTC 1 cut(s) 158
BglII AGATCT 1 cut(s) 94
BisI GCNGC 1 cut(s) 247
BlsI GCNGC 1 cut(s) 248
Bme1390I CCNGG 2 cut(s) 275, 334
BmgBI CACGTC 1 cut(s) 199
BmiI GGNNCC 2 cut(s) 312, 471
BmrFI CCNGG 2 cut(s) 275, 334
BmsI GCATC 1 cut(s) 31
BpuEI CTTGAG 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 332
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 198
Bse3DI GCAATG 1 cut(s) 117
BseBI CCWGG 2 cut(s) 275, 334
BseDI CCNNGG 1 cut(s) 332
BseGI GGATG 1 cut(s) 34
BseLI CCNNNNNNNGG 1 cut(s) 112
BseMI GCAATG 1 cut(s) 117
BseNI ACTGG 1 cut(s) 198
BseRI GAGGAG 3 cut(s) 176, 233, 363
BseXI GCAGC 1 cut(s) 258
BshFI GGCC 1 cut(s) 53
BslI CCNNNNNNNGG 1 cut(s) 112
BsmAI GTCTC 1 cut(s) 158
BsnI GGCC 1 cut(s) 53
Bso31I GGTCTC 1 cut(s) 158
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 5 cut(s) 94, 310, 356, 469, 493
BspACI CCGC 1 cut(s) 489
BspANI GGCC 1 cut(s) 53
BspLI GGNNCC 2 cut(s) 312, 471
BspPI GGATC 5 cut(s) 305, 318, 364, 464, 477
BspQI GCTCTTC 1 cut(s) 285
BspTNI GGTCTC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 117
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 1 cut(s) 198
BssECI CCNNGG 1 cut(s) 332
BssMI GATC 5 cut(s) 94, 310, 356, 469, 493
Bst2UI CCWGG 2 cut(s) 275, 334
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 1 cut(s) 285
BstAUI TGTACA 1 cut(s) 477
BstC8I GCNNGC 1 cut(s) 271
BstF5I GGATG 1 cut(s) 34
BstKTI GATC 5 cut(s) 97, 313, 359, 472, 496
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 5 cut(s) 94, 310, 356, 469, 493
BstMWI GCNNNNNNNGC 3 cut(s) 50, 275, 304
BstNI CCWGG 2 cut(s) 275, 334
BstNSI RCATGY 1 cut(s) 273
BstSCI CCNGG 2 cut(s) 273, 332
BstV1I GCAGC 1 cut(s) 258
BstX2I RGATCY 3 cut(s) 94, 310, 469
BstYI RGATCY 3 cut(s) 94, 310, 469
BsuRI GGCC 1 cut(s) 53
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 1 cut(s) 34
Cac8I GCNNGC 1 cut(s) 271
CaiI CAGNNNCTG 1 cut(s) 392
Csp6I GTAC 1 cut(s) 478
CviAII CATG 5 cut(s) 150, 258, 270, 360, 475
CviJI RGCY 6 cut(s) 53, 121, 249, 278, 298, 307
CviKI_1 RGCY 6 cut(s) 53, 121, 249, 278, 298, 307
CviQI GTAC 1 cut(s) 478
DpnI GATC 5 cut(s) 96, 312, 358, 471, 495
DpnII GATC 5 cut(s) 94, 310, 356, 469, 493
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco31I GGTCTC 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 273, 332
FaeI CATG 5 cut(s) 153, 261, 273, 363, 478
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FatI CATG 5 cut(s) 149, 257, 269, 359, 474
FauNDI CATATG 1 cut(s) 367
Fnu4HI GCNGC 1 cut(s) 247
FokI GGATG 1 cut(s) 41
Fsp4HI GCNGC 1 cut(s) 247
GluI GCNGC 1 cut(s) 247
HaeIII GGCC 1 cut(s) 53
Hin1II CATG 5 cut(s) 153, 261, 273, 363, 478
HinfI GANTC 4 cut(s) 79, 127, 209, 326
HphI GGTGA 1 cut(s) 321
Hpy188I TCNGA 4 cut(s) 160, 315, 394, 423
Hpy188III TCNNGA 1 cut(s) 184
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 3 cut(s) 66, 261, 399
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 275, 304
HpySE526I ACGT 1 cut(s) 198
Hsp92II CATG 5 cut(s) 153, 261, 273, 363, 478
Kzo9I GATC 5 cut(s) 94, 310, 356, 469, 493
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 376
LpnPI CCDG 8 cut(s) 179, 260, 280, 287, 293, 319, 339, 346
Lsp1109I GCAGC 1 cut(s) 258
LweI GCATC 1 cut(s) 31
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 184
MalI GATC 5 cut(s) 96, 312, 358, 471, 495
MboI GATC 5 cut(s) 94, 310, 356, 469, 493
MboII GAAGA 3 cut(s) 32, 272, 440
MflI RGATCY 3 cut(s) 94, 310, 469
MluCI AATT 3 cut(s) 286, 346, 417
MlyI GAGTC 2 cut(s) 73, 136
MnlI CCTC 5 cut(s) 19, 154, 211, 225, 384
MroXI GAANNNNTTC 1 cut(s) 239
MspR9I CCNGG 2 cut(s) 275, 334
MvaI CCWGG 2 cut(s) 275, 334
MwoI GCNNNNNNNGC 3 cut(s) 50, 275, 304
NdeI CATATG 1 cut(s) 367
NdeII GATC 5 cut(s) 94, 310, 356, 469, 493
NlaIII CATG 5 cut(s) 153, 261, 273, 363, 478
NlaIV GGNNCC 2 cut(s) 312, 471
NmuCI GTSAC 1 cut(s) 184
NspI RCATGY 1 cut(s) 273
PaeI GCATGC 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 285
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 239
PfeI GAWTC 2 cut(s) 209, 326
PflMI CCANNNNNTGG 1 cut(s) 112
PkrI GCNGC 1 cut(s) 248
PleI GAGTC 2 cut(s) 73, 135
PpsI GAGTC 2 cut(s) 73, 135
Psp6I CCWGG 2 cut(s) 273, 332
PspGI CCWGG 2 cut(s) 273, 332
PspN4I GGNNCC 2 cut(s) 312, 471
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 3 cut(s) 94, 310, 469
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
SapI GCTCTTC 1 cut(s) 285
SatI GCNGC 1 cut(s) 247
Sau3AI GATC 5 cut(s) 94, 310, 356, 469, 493
SchI GAGTC 2 cut(s) 73, 136
ScrFI CCNGG 2 cut(s) 275, 334
SetI ASST 3 cut(s) 201, 251, 309
SfaNI GCATC 1 cut(s) 31
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
SphI GCATGC 1 cut(s) 273
Sse9I AATT 3 cut(s) 286, 346, 417
SsiI CCGC 1 cut(s) 489
StyD4I CCNGG 2 cut(s) 273, 332
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 1 cut(s) 201
TasI AATT 3 cut(s) 286, 346, 417
TatI WGTACW 1 cut(s) 477
TfiI GAWTC 2 cut(s) 209, 326
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 1 cut(s) 246
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 2 cut(s) 33, 359
TspGWI ACGGA 1 cut(s) 366
Van91I CCANNNNNTGG 1 cut(s) 112
XapI RAATTY 1 cut(s) 286
XceI RCATGY 1 cut(s) 273
XcmI CCANNNNNNNNNTGG 2 cut(s) 129, 196
XmnI GAANNNNTTC 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.