Rroxscaffold_5G00376270

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007348
Physical Location & Seq
Forward (+)
56985001 .. 56987646
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00376270.1

Sequence Viewer

Length: 948 bp
ATGTCTCTCTTGAACCAGCTCTTCAATAGAGGCGTTTTCGGCACAAAATGCAAAACATGTCTGACCTTGGCAATTTCGCGGATTAAGTTGCTGCAAAACAAGAGAGATATGCAGCTTAAACATATGCGCAAGGAGATTGCTCAGTTTTTGCAGGCTGGCCAAGAAGCAATAGCTCGAATTCGGGTGGAGCATGTTATACGAGAGCAAAACATATGGGCTGCTTATGAGATATTGGAGCTTTTCTGTGAATTTGTCCTCGCACGGGTTCCCATTATTGAGAGCCAAAAGGATTGCCCACCTGAACTACGAGAGGCTATATCAAGCATAATTTTTTCTGCCCCAAGATGTTCAGAAGTTCCGGATTTATTGCAGATCAAGAATTTGTTTACAGCAAAATATGGGAAGGAATTTACAGCATCTGCCTCTGAGCTTCGTCCTGATTCTGGTGTCAACCGAACTATAATTGAAAAGCTTTCAGTTGGTGCTCCCTCGGGAGAGGCAAGGCTCAAGGTATTGAAGGAAATCGCACAAGAGTACAATCTGAATTGGGATTCTTCTAGCACGGAAGCGGAATTCAGTAGAAAACATGAAGATCTGCTGGGTGGATCCAAGCACATAAGTGGTGGAGCTGCACTGCCTTCTCGAGCTCCTGCTCCTGCAAATCAAGTGTCTTTTAGTTCTCCGCCTTCTAATGGTGCACGTTATATTCAGCCCGCAGAGATTAAACCAGAACCTCAACAACACATTCCTGCTCCAAGATCTTTAATTCAGACGCATGTGTCAAGTACCAATGAGATTCAGCCATCAATTAATAATGATGCAGTACCAGTTAGGGAAACAAGACCACAATCATCTGAGATCCTGGAGAAAGCTCGAGCTGCCATCGCGTCTGCTGAACGCGCATCTGCTGCTGCCCGTTCAGCTGCTGAGCTGGTTAAGTCATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

315

Amino Acids

34.71

Weight (kDa)

8.93

Isoelectric Point (pI)

57.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 2.6e-65 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 778
Acc16I TGCGCA 1 cut(s) 128
AccII CGCG 3 cut(s) 79, 887, 900
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 4 cut(s) 79, 569, 683, 714
AclWI GGATC 3 cut(s) 600, 613, 853
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 5 cut(s) 177, 248, 379, 407, 572
AfaI GTAC 3 cut(s) 536, 787, 825
AfiI CCNNNNNNNGG 3 cut(s) 262, 443, 692
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 4 cut(s) 13, 25, 467, 517
AjnI CCWGG 1 cut(s) 861
AleI CACNNNNGTG 1 cut(s) 618
Alw21I GWGCWC 3 cut(s) 487, 649, 700
Alw26I GTCTC 1 cut(s) 9
Alw44I GTGCAC 1 cut(s) 696
AlwI GGATC 3 cut(s) 600, 613, 853
AlwNI CAGNNNCTG 2 cut(s) 419, 926
Ama87I CYCGRG 3 cut(s) 490, 642, 873
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 1 cut(s) 157
ApaLI GTGCAC 1 cut(s) 696
ApeKI GCWGC 8 cut(s) 91, 112, 218, 629, 878, 908, 911, 923
ApoI RAATTY 5 cut(s) 177, 248, 379, 407, 572
ArsI GACNNNNNNTTYG 2 cut(s) 653, 685
AseI ATTAAT 1 cut(s) 810
AspLEI GCGC 2 cut(s) 129, 902
AvaI CYCGRG 3 cut(s) 490, 642, 873
BaeGI GKGCMC 1 cut(s) 700
BalI TGGCCA 1 cut(s) 159
BamHI GGATCC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 649
Bbv12I GWGCWC 3 cut(s) 487, 649, 700
BbvI GCAGC 8 cut(s) 78, 124, 205, 616, 865, 895, 898, 910
BccI CCATC 2 cut(s) 811, 890
BciT130I CCWGG 1 cut(s) 863
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 558
BglII AGATCT 2 cut(s) 592, 758
BisI GCNGC 8 cut(s) 92, 113, 219, 630, 879, 909, 912, 924
BlpI GCTNAGC 1 cut(s) 927
BlsI GCNGC 8 cut(s) 93, 114, 220, 631, 880, 910, 913, 925
Bme1390I CCNGG 1 cut(s) 863
BmeT110I CYCGRG 3 cut(s) 490, 642, 873
BmiI GGNNCC 2 cut(s) 267, 607
BmrFI CCNGG 1 cut(s) 863
BmsI GCATC 3 cut(s) 425, 808, 911
BpmI CTGGAG 1 cut(s) 884
Bpu1102I GCTNAGC 1 cut(s) 927
BpuEI CTTGAG 1 cut(s) 491
BsaJI CCNNGG 2 cut(s) 66, 489
BsaWI WCCGGW 1 cut(s) 358
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 443, 692
Bse1I ACTGG 1 cut(s) 827
BseAI TCCGGA 1 cut(s) 358
BseBI CCWGG 1 cut(s) 863
BseDI CCNNGG 2 cut(s) 66, 489
BseLI CCNNNNNNNGG 3 cut(s) 262, 443, 692
BseMII CTCAG 4 cut(s) 155, 417, 846, 918
BseNI ACTGG 1 cut(s) 827
BseSI GKGCMC 1 cut(s) 700
BseXI GCAGC 8 cut(s) 78, 124, 205, 616, 865, 895, 898, 910
BseYI CCCAGC 1 cut(s) 598
BsgI GTGCAG 1 cut(s) 615
Bsh1236I CGCG 3 cut(s) 79, 887, 900
BshFI GGCC 1 cut(s) 159
BsiHKAI GWGCWC 3 cut(s) 487, 649, 700
BsiHKCI CYCGRG 3 cut(s) 490, 642, 873
BsiSI CCGG 1 cut(s) 359
BslI CCNNNNNNNGG 3 cut(s) 262, 443, 692
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 159
BsoBI CYCGRG 3 cut(s) 490, 642, 873
Bsp1286I GDGCHC 3 cut(s) 487, 649, 700
Bsp13I TCCGGA 1 cut(s) 358
Bsp143I GATC 5 cut(s) 372, 592, 605, 758, 858
Bsp1720I GCTNAGC 1 cut(s) 927
BspACI CCGC 4 cut(s) 79, 569, 683, 714
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 4 cut(s) 154, 418, 847, 919
BspEI TCCGGA 1 cut(s) 358
BspFNI CGCG 3 cut(s) 79, 887, 900
BspLI GGNNCC 2 cut(s) 267, 607
BspPI GGATC 3 cut(s) 600, 613, 853
BspQI GCTCTTC 1 cut(s) 26
BsrI ACTGG 1 cut(s) 827
BssECI CCNNGG 2 cut(s) 66, 489
BssMI GATC 5 cut(s) 372, 592, 605, 758, 858
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 863
Bst6I CTCTTC 1 cut(s) 26
BstAPI GCANNNNNTGC 2 cut(s) 48, 908
BstC8I GCNNGC 3 cut(s) 153, 157, 714
BstDEI CTNAG 4 cut(s) 141, 426, 855, 927
BstFNI CGCG 3 cut(s) 79, 887, 900
BstHHI GCGC 2 cut(s) 129, 902
BstKTI GATC 5 cut(s) 375, 595, 608, 761, 861
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 5 cut(s) 372, 592, 605, 758, 858
BstMWI GCNNNNNNNGC 7 cut(s) 39, 48, 878, 884, 899, 908, 920
BstNI CCWGG 1 cut(s) 863
BstNSI RCATGY 3 cut(s) 60, 194, 779
BstSCI CCNGG 1 cut(s) 861
BstSLI GKGCMC 1 cut(s) 700
BstUI CGCG 3 cut(s) 79, 887, 900
BstV1I GCAGC 8 cut(s) 78, 124, 205, 616, 865, 895, 898, 910
BstX2I RGATCY 4 cut(s) 592, 605, 758, 858
BstYI RGATCY 4 cut(s) 592, 605, 758, 858
BsuRI GGCC 1 cut(s) 159
BtgZI GCGATG 1 cut(s) 868
BtsI GCAGTG 1 cut(s) 632
BtsIMutI CAGTG 1 cut(s) 632
Cac8I GCNNGC 3 cut(s) 153, 157, 714
CaiI CAGNNNCTG 2 cut(s) 419, 926
CfoI GCGC 2 cut(s) 129, 902
CseI GACGC 2 cut(s) 781, 876
Csp6I GTAC 3 cut(s) 535, 786, 824
CviAII CATG 4 cut(s) 57, 191, 587, 776
CviQI GTAC 3 cut(s) 535, 786, 824
DdeI CTNAG 4 cut(s) 141, 426, 855, 927
DpnI GATC 5 cut(s) 374, 594, 607, 760, 860
DpnII GATC 5 cut(s) 372, 592, 605, 758, 858
DrdI GACNNNNNNGTC 1 cut(s) 778
DseDI GACNNNNNNGTC 1 cut(s) 778
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 672
Ecl136II GAGCTC 1 cut(s) 647
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 649
Eco53kI GAGCTC 1 cut(s) 647
Eco88I CYCGRG 3 cut(s) 490, 642, 873
EcoICRI GAGCTC 1 cut(s) 647
EcoRI GAATTC 2 cut(s) 177, 572
EcoRII CCWGG 1 cut(s) 861
EcoT14I CCWWGG 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 649
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 4 cut(s) 60, 194, 590, 779
FatI CATG 4 cut(s) 56, 190, 586, 775
FauI CCCGC 1 cut(s) 721
FauNDI CATATG 2 cut(s) 123, 212
Fnu4HI GCNGC 8 cut(s) 92, 113, 219, 630, 879, 909, 912, 924
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 8 cut(s) 92, 113, 219, 630, 879, 909, 912, 924
FspBI CTAG 1 cut(s) 558
FspI TGCGCA 1 cut(s) 128
GlaI GCGC 2 cut(s) 128, 901
GluI GCNGC 8 cut(s) 92, 113, 219, 630, 879, 909, 912, 924
GsaI CCCAGC 1 cut(s) 602
GsuI CTGGAG 1 cut(s) 884
HaeIII GGCC 1 cut(s) 159
HapII CCGG 1 cut(s) 359
HgaI GACGC 2 cut(s) 781, 876
HhaI GCGC 2 cut(s) 129, 902
Hin1II CATG 4 cut(s) 60, 194, 590, 779
Hin6I GCGC 2 cut(s) 127, 900
HinP1I GCGC 2 cut(s) 127, 900
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 3 cut(s) 440, 551, 796
HpaII CCGG 1 cut(s) 359
Hpy166II GTNNAC 3 cut(s) 387, 451, 698
Hpy188I TCNGA 6 cut(s) 63, 352, 427, 543, 771, 856
Hpy188III TCNNGA 6 cut(s) 10, 359, 376, 437, 492, 642
Hpy8I GTNNAC 3 cut(s) 387, 451, 698
HpyAV CCTTC 4 cut(s) 397, 511, 648, 696
HpyCH4IV ACGT 1 cut(s) 700
HpyCH4V TGCA 9 cut(s) 51, 94, 112, 151, 370, 632, 659, 698, 821
HpyF10VI GCNNNNNNNGC 7 cut(s) 39, 48, 878, 884, 899, 908, 920
HpyF3I CTNAG 4 cut(s) 141, 426, 855, 927
HpySE526I ACGT 1 cut(s) 700
Hsp92II CATG 4 cut(s) 60, 194, 590, 779
HspAI GCGC 2 cut(s) 127, 900
Kpn2I TCCGGA 1 cut(s) 358
Kzo9I GATC 5 cut(s) 372, 592, 605, 758, 858
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 7 cut(s) 187, 235, 490, 626, 652, 658, 757
Lsp1109I GCAGC 8 cut(s) 78, 124, 205, 616, 865, 895, 898, 910
LweI GCATC 3 cut(s) 425, 808, 911
MaeI CTAG 1 cut(s) 558
MaeII ACGT 1 cut(s) 700
MalI GATC 5 cut(s) 374, 594, 607, 760, 860
MboI GATC 5 cut(s) 372, 592, 605, 758, 858
MboII GAAGA 3 cut(s) 13, 546, 602
MflI RGATCY 4 cut(s) 592, 605, 758, 858
MhlI GDGCHC 3 cut(s) 487, 649, 700
MlsI TGGCCA 1 cut(s) 159
MluNI TGGCCA 1 cut(s) 159
MnlI CCTC 7 cut(s) 23, 266, 304, 433, 490, 499, 744
Mox20I TGGCCA 1 cut(s) 159
MroI TCCGGA 1 cut(s) 358
MscI TGGCCA 1 cut(s) 159
MseI TTAA 6 cut(s) 84, 117, 723, 764, 810, 936
MslI CAYNNNNRTG 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 923
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 1 cut(s) 863
MvaI CCWGG 1 cut(s) 863
MvnI CGCG 3 cut(s) 79, 887, 900
MwoI GCNNNNNNNGC 7 cut(s) 39, 48, 878, 884, 899, 908, 920
NdeI CATATG 2 cut(s) 123, 212
NdeII GATC 5 cut(s) 372, 592, 605, 758, 858
NlaIII CATG 4 cut(s) 60, 194, 590, 779
NlaIV GGNNCC 2 cut(s) 267, 607
NsbI TGCGCA 1 cut(s) 128
NspI RCATGY 3 cut(s) 60, 194, 779
OliI CACNNNNGTG 1 cut(s) 618
PaeR7I CTCGAG 2 cut(s) 642, 873
PciI ACATGT 1 cut(s) 56
PciSI GCTCTTC 1 cut(s) 26
PfeI GAWTC 3 cut(s) 440, 551, 796
PfoI TCCNGGA 1 cut(s) 861
PkrI GCNGC 8 cut(s) 93, 114, 220, 631, 880, 910, 913, 925
PscI ACATGT 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 810
Psp124BI GAGCTC 1 cut(s) 649
Psp6I CCWGG 1 cut(s) 861
PspFI CCCAGC 1 cut(s) 598
PspGI CCWGG 1 cut(s) 861
PspN4I GGNNCC 2 cut(s) 267, 607
PspXI VCTCGAGB 1 cut(s) 873
PstNI CAGNNNCTG 2 cut(s) 419, 926
PsuI RGATCY 4 cut(s) 592, 605, 758, 858
PvuII CAGCTG 1 cut(s) 923
RsaI GTAC 3 cut(s) 536, 787, 825
RsaNI GTAC 3 cut(s) 535, 786, 824
RseI CAYNNNNRTG 1 cut(s) 618
SacI GAGCTC 1 cut(s) 649
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 6 cut(s) 84, 117, 723, 764, 810, 936
SatI GCNGC 8 cut(s) 92, 113, 219, 630, 879, 909, 912, 924
Sau3AI GATC 5 cut(s) 372, 592, 605, 758, 858
ScrFI CCNGG 1 cut(s) 863
SduI GDGCHC 3 cut(s) 487, 649, 700
SfaNI GCATC 3 cut(s) 425, 808, 911
Sfr274I CTCGAG 2 cut(s) 642, 873
SlaI CTCGAG 2 cut(s) 642, 873
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 3 cut(s) 506, 642, 873
SmoI CTYRAG 3 cut(s) 506, 642, 873
SsiI CCGC 4 cut(s) 79, 569, 683, 714
SspMI CTAG 1 cut(s) 558
SstI GAGCTC 1 cut(s) 649
StyD4I CCNGG 1 cut(s) 861
StyI CCWWGG 1 cut(s) 66
TaiI ACGT 1 cut(s) 703
TaqI TCGA 3 cut(s) 175, 643, 874
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 3 cut(s) 440, 551, 796
Tru1I TTAA 6 cut(s) 84, 117, 723, 764, 810, 936
Tru9I TTAA 6 cut(s) 84, 117, 723, 764, 810, 936
TscAI CASTG 1 cut(s) 639
TseI GCWGC 8 cut(s) 91, 112, 218, 629, 878, 908, 911, 923
TspDTI ATGAA 1 cut(s) 603
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 1 cut(s) 639
VneI GTGCAC 1 cut(s) 696
VspI ATTAAT 1 cut(s) 810
XapI RAATTY 5 cut(s) 177, 248, 379, 407, 572
XceI RCATGY 3 cut(s) 60, 194, 779
XhoI CTCGAG 2 cut(s) 642, 873
XspI CTAG 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.