Rh4BG345600

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007348
Physical Location & Seq
Forward (+)
50834568 .. 50837539
2972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG345600.1

Sequence Viewer

Length: 702 bp
ATGCAATTGCTACTCAATTATCTGAACCACTTTTCTCCTTATTACAGGGATTGCCCACCAGAACTACGAGAGGCTATATCAAGCATAATTTTTTCTGCCCCAAGATGTTCAGAAGTTCCGGATTTATTGCAGATCAAGAATTTGTTTACAGCAAAATATGGGAAGGAATTTATAGCATCTGCCTCTGAGCTTCGTCCTGATTCTGGTGTCAACCGAACTATAATTGAAAAGCTTTCAGTTAGTGCTCCCTCTGGAGAGGCAAGGCTCAAGGTATTGAAGGAAATCGCACAAGAGTACAATCTGAATTGGGATTCTTCTAGCACGGAAGCGGAATTCAGTAGAAAACATGAAGATCTGCTGGGTGGATCCAAGCACATAAGTGGTGGAGCTGCACTGCCTTCTCGAGCTCCTGCAAATCAAGTGTCTTTTAGTTCTCCGCCTTCTAATGGTGCACATTATATTCAGACCGCAGAGATTAAACCAGAACCTCAACAACACATTCCTGCTCCAAGATCTTTAATTCAGACGCATGTGTCAAGTACCGATGAGATTCAGCCATCAATTAATAATGATGCAGTACCAGTTAGGGAAACAAGACCACAATCATCTGTGATCCTGGAGAAAGCTCGAGCTGCCATTGCGTCTGCTGAACGCGCATGTGCTGCTGCCCGTTCAGCTGCTGAGCTGGTTAAGTCATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

233

Amino Acids

25.39

Weight (kDa)

6.6

Isoelectric Point (pI)

61.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 8 - 101 6.6e-30 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 532
AccII CGCG 1 cut(s) 654
AccIII TCCGGA 1 cut(s) 118
AciI CCGC 3 cut(s) 329, 437, 468
AclWI GGATC 3 cut(s) 360, 373, 607
AcsI RAATTY 3 cut(s) 139, 167, 332
AfaI GTAC 3 cut(s) 296, 541, 579
AfiI CCNNNNNNNGG 2 cut(s) 203, 446
AgsI TTSAA 2 cut(s) 227, 277
AjnI CCWGG 1 cut(s) 615
AleI CACNNNNGTG 1 cut(s) 378
AluBI AGCT 8 cut(s) 190, 232, 389, 407, 626, 632, 677, 685
AluI AGCT 8 cut(s) 190, 232, 389, 407, 626, 632, 677, 685
Alw21I GWGCWC 3 cut(s) 247, 409, 454
Alw44I GTGCAC 1 cut(s) 450
AlwI GGATC 3 cut(s) 360, 373, 607
AlwNI CAGNNNCTG 1 cut(s) 680
Ama87I CYCGRG 2 cut(s) 402, 627
Aor13HI TCCGGA 1 cut(s) 118
ApaLI GTGCAC 1 cut(s) 450
ApeKI GCWGC 5 cut(s) 389, 632, 662, 665, 677
ApoI RAATTY 3 cut(s) 139, 167, 332
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AseI ATTAAT 1 cut(s) 564
AspLEI GCGC 1 cut(s) 656
AvaI CYCGRG 2 cut(s) 402, 627
BaeGI GKGCMC 1 cut(s) 454
BamHI GGATCC 1 cut(s) 365
BanII GRGCYC 1 cut(s) 409
Bbv12I GWGCWC 3 cut(s) 247, 409, 454
BbvI GCAGC 5 cut(s) 376, 619, 649, 652, 664
BccI CCATC 1 cut(s) 565
BciT130I CCWGG 1 cut(s) 617
BfaI CTAG 1 cut(s) 318
BglII AGATCT 2 cut(s) 352, 512
BisI GCNGC 5 cut(s) 390, 633, 663, 666, 678
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 5 cut(s) 391, 634, 664, 667, 679
Bme1390I CCNGG 1 cut(s) 617
BmeT110I CYCGRG 2 cut(s) 402, 627
BmiI GGNNCC 1 cut(s) 367
BmrFI CCNGG 1 cut(s) 617
BmsI GCATC 2 cut(s) 185, 562
BpmI CTGGAG 2 cut(s) 273, 638
Bpu1102I GCTNAGC 1 cut(s) 681
BpuEI CTTGAG 1 cut(s) 251
BsaWI WCCGGW 1 cut(s) 118
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 446
Bse1I ACTGG 1 cut(s) 581
Bse3DI GCAATG 1 cut(s) 636
BseAI TCCGGA 1 cut(s) 118
BseBI CCWGG 1 cut(s) 617
BseLI CCNNNNNNNGG 2 cut(s) 203, 446
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 2 cut(s) 177, 672
BseNI ACTGG 1 cut(s) 581
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 5 cut(s) 376, 619, 649, 652, 664
BseYI CCCAGC 1 cut(s) 358
BsgI GTGCAG 1 cut(s) 375
Bsh1236I CGCG 1 cut(s) 654
BsiHKAI GWGCWC 3 cut(s) 247, 409, 454
BsiHKCI CYCGRG 2 cut(s) 402, 627
BsiSI CCGG 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 203, 446
BsoBI CYCGRG 2 cut(s) 402, 627
Bsp1286I GDGCHC 3 cut(s) 247, 409, 454
Bsp13I TCCGGA 1 cut(s) 118
Bsp143I GATC 5 cut(s) 132, 352, 365, 512, 612
Bsp1720I GCTNAGC 1 cut(s) 681
BspACI CCGC 3 cut(s) 329, 437, 468
BspCNI CTCAG 2 cut(s) 178, 673
BspEI TCCGGA 1 cut(s) 118
BspFNI CGCG 1 cut(s) 654
BspLI GGNNCC 1 cut(s) 367
BspPI GGATC 3 cut(s) 360, 373, 607
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 1 cut(s) 581
BssMI GATC 5 cut(s) 132, 352, 365, 512, 612
Bst2UI CCWGG 1 cut(s) 617
BstAPI GCANNNNNTGC 1 cut(s) 662
BstDEI CTNAG 2 cut(s) 186, 681
BstFNI CGCG 1 cut(s) 654
BstHHI GCGC 1 cut(s) 656
BstKTI GATC 5 cut(s) 135, 355, 368, 515, 615
BstMBI GATC 5 cut(s) 132, 352, 365, 512, 612
BstMWI GCNNNNNNNGC 5 cut(s) 632, 638, 653, 662, 674
BstNI CCWGG 1 cut(s) 617
BstNSI RCATGY 2 cut(s) 533, 660
BstSCI CCNGG 1 cut(s) 615
BstSLI GKGCMC 1 cut(s) 454
BstUI CGCG 1 cut(s) 654
BstV1I GCAGC 5 cut(s) 376, 619, 649, 652, 664
BstX2I RGATCY 3 cut(s) 352, 365, 512
BstYI RGATCY 3 cut(s) 352, 365, 512
BtsI GCAGTG 1 cut(s) 392
BtsIMutI CAGTG 1 cut(s) 392
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 1 cut(s) 656
CseI GACGC 2 cut(s) 535, 630
Csp6I GTAC 3 cut(s) 295, 540, 578
CviAII CATG 3 cut(s) 347, 530, 657
CviQI GTAC 3 cut(s) 295, 540, 578
DdeI CTNAG 2 cut(s) 186, 681
DpnI GATC 5 cut(s) 134, 354, 367, 514, 614
DpnII GATC 5 cut(s) 132, 352, 365, 512, 612
DrdI GACNNNNNNGTC 1 cut(s) 532
DseDI GACNNNNNNGTC 1 cut(s) 532
EciI GGCGGA 1 cut(s) 426
Ecl136II GAGCTC 1 cut(s) 407
Eco24I GRGCYC 1 cut(s) 409
Eco53kI GAGCTC 1 cut(s) 407
Eco88I CYCGRG 2 cut(s) 402, 627
EcoICRI GAGCTC 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 615
EcoT38I GRGCYC 1 cut(s) 409
FaeI CATG 3 cut(s) 350, 533, 660
FatI CATG 3 cut(s) 346, 529, 656
Fnu4HI GCNGC 5 cut(s) 390, 633, 663, 666, 678
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 5 cut(s) 390, 633, 663, 666, 678
FspBI CTAG 1 cut(s) 318
GlaI GCGC 1 cut(s) 655
GluI GCNGC 5 cut(s) 390, 633, 663, 666, 678
GsaI CCCAGC 1 cut(s) 362
GsuI CTGGAG 2 cut(s) 273, 638
HapII CCGG 1 cut(s) 119
HgaI GACGC 2 cut(s) 535, 630
HhaI GCGC 1 cut(s) 656
Hin1II CATG 3 cut(s) 350, 533, 660
Hin6I GCGC 1 cut(s) 654
HinP1I GCGC 1 cut(s) 654
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 3 cut(s) 200, 311, 550
HpaII CCGG 1 cut(s) 119
Hpy166II GTNNAC 3 cut(s) 147, 211, 452
Hpy188I TCNGA 6 cut(s) 24, 112, 187, 303, 465, 525
Hpy188III TCNNGA 5 cut(s) 119, 136, 197, 252, 402
Hpy8I GTNNAC 3 cut(s) 147, 211, 452
HpyAV CCTTC 4 cut(s) 157, 271, 408, 450
HpyCH4V TGCA 6 cut(s) 4, 130, 392, 413, 452, 575
HpyF10VI GCNNNNNNNGC 5 cut(s) 632, 638, 653, 662, 674
HpyF3I CTNAG 2 cut(s) 186, 681
Hsp92II CATG 3 cut(s) 350, 533, 660
HspAI GCGC 1 cut(s) 654
Kpn2I TCCGGA 1 cut(s) 118
Kzo9I GATC 5 cut(s) 132, 352, 365, 512, 612
LmnI GCTCC 4 cut(s) 250, 386, 412, 511
Lsp1109I GCAGC 5 cut(s) 376, 619, 649, 652, 664
LweI GCATC 2 cut(s) 185, 562
MaeI CTAG 1 cut(s) 318
MalI GATC 5 cut(s) 134, 354, 367, 514, 614
MboI GATC 5 cut(s) 132, 352, 365, 512, 612
MboII GAAGA 2 cut(s) 306, 362
MfeI CAATTG 1 cut(s) 5
MflI RGATCY 3 cut(s) 352, 365, 512
MhlI GDGCHC 3 cut(s) 247, 409, 454
MnlI CCTC 5 cut(s) 64, 193, 250, 259, 498
MroI TCCGGA 1 cut(s) 118
MseI TTAA 4 cut(s) 477, 518, 564, 690
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 617
MunI CAATTG 1 cut(s) 5
MvaI CCWGG 1 cut(s) 617
MvnI CGCG 1 cut(s) 654
MwoI GCNNNNNNNGC 5 cut(s) 632, 638, 653, 662, 674
NdeII GATC 5 cut(s) 132, 352, 365, 512, 612
NlaIII CATG 3 cut(s) 350, 533, 660
NlaIV GGNNCC 1 cut(s) 367
NspI RCATGY 2 cut(s) 533, 660
OliI CACNNNNGTG 1 cut(s) 378
PaeR7I CTCGAG 2 cut(s) 402, 627
PfeI GAWTC 3 cut(s) 200, 311, 550
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 5 cut(s) 391, 634, 664, 667, 679
PshBI ATTAAT 1 cut(s) 564
Psp124BI GAGCTC 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 615
PspFI CCCAGC 1 cut(s) 358
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 1 cut(s) 367
PspXI VCTCGAGB 1 cut(s) 627
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 3 cut(s) 352, 365, 512
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 3 cut(s) 296, 541, 579
RsaNI GTAC 3 cut(s) 295, 540, 578
RseI CAYNNNNRTG 1 cut(s) 378
SacI GAGCTC 1 cut(s) 409
SaqAI TTAA 4 cut(s) 477, 518, 564, 690
SatI GCNGC 5 cut(s) 390, 633, 663, 666, 678
Sau3AI GATC 5 cut(s) 132, 352, 365, 512, 612
ScrFI CCNGG 1 cut(s) 617
SduI GDGCHC 3 cut(s) 247, 409, 454
SfaNI GCATC 2 cut(s) 185, 562
Sfr274I CTCGAG 2 cut(s) 402, 627
SlaI CTCGAG 2 cut(s) 402, 627
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 3 cut(s) 266, 402, 627
SmoI CTYRAG 3 cut(s) 266, 402, 627
SsiI CCGC 3 cut(s) 329, 437, 468
SspMI CTAG 1 cut(s) 318
SstI GAGCTC 1 cut(s) 409
StyD4I CCNGG 1 cut(s) 615
TaqI TCGA 2 cut(s) 403, 628
TatI WGTACW 1 cut(s) 294
TfiI GAWTC 3 cut(s) 200, 311, 550
Tru1I TTAA 4 cut(s) 477, 518, 564, 690
Tru9I TTAA 4 cut(s) 477, 518, 564, 690
TscAI CASTG 1 cut(s) 399
TseI GCWGC 5 cut(s) 389, 632, 662, 665, 677
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 1 cut(s) 399
VneI GTGCAC 1 cut(s) 450
VspI ATTAAT 1 cut(s) 564
XapI RAATTY 3 cut(s) 139, 167, 332
XceI RCATGY 2 cut(s) 533, 660
XhoI CTCGAG 2 cut(s) 402, 627
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.