MD16G1042100.v1.1

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007348
Physical Location & Seq
Forward (+)
2962258 .. 2965140
2883 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1042100.v1.1.491

Sequence Viewer

Length: 948 bp
ATGTCTCTGTTAAACCAGCTCTTCAACAGAGGCGTTTTCGGCACAAAATGCAAAACATGTTTGAATTTGGGTATTTCACGCATTAAATTGCTGCAAAACAAGAGAGATGTGCAGCTCAAACATATGCGCAAGGAGATTGCGCAGTTTTTGCAGGCTGGCCAAGAAGCAATAGCTCGAATTCGGGTGGAGCATGTTATACGAGAGCAAAACATTTGGGCTGCTTATGAGATATTGGAGCTGTTCTGTGAATTTGTACTTGCACGGGTTCCCATTATTGAAAATCAGAGGGAGTGTCCAACGGAATTACGAGAAGCTATATCGAGCATAATTTTTGCCGCCCCAAGATGCTCAGATGTTCCAGATTTAATGCAGATCACAAATTTGTTTACAGCAAAATATGGGAAGGAATTTGTATCAGCTGCGTCTGAGCTTCGTCCTGATTCTGGTGTCAACAGAACAATAATTGAAAAGCTTTCAGTTAGTGCTCCGTCTGGAGAGGCAAGGCTTAAGGTATTGAAGGAAATTGCACAAGAGTACAAGCTGAATTGGGATTCTTCTGACACTGAAGCGGAATTCAGTAAAAAGCATGACGATCTGCTGGGTGGATTGAAGCATGTAAGTGGTGCTGCTGCACCTTCTCAAGCTCCCGCAAAACGAGTAACTTTTAGTTCCCCACCTTCTAATGGGGCACATACTATTGTGCCTGCAGATGTTAAACAAGAACCTCAATACCTCCGTGCTCCAAGATCTTTAATCAACACACCATCGTTCAGTAGTGAGATTCAACCATCAGGGAATGATGATAAAGCAAGGCCAGTTAGTGAAACCAAAGGGGAGACAAGACCACAATCATCTGATATCTTGGACAGAGCTCGAGCTGCCATTGCCTCTGCTGAGCGTGCATCTGCTGCTGCCCGTCGGGCTGCTGAGCTGGTTAAGTCCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

316

Amino Acids

34.78

Weight (kDa)

9.04

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 18 - 181 2.9e-65 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 128, 141
AciI CCGC 3 cut(s) 336, 569, 648
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 6 cut(s) 64, 177, 248, 379, 407, 572
AcuI CTGAAG 1 cut(s) 585
AfaI GTAC 2 cut(s) 255, 536
AfiI CCNNNNNNNGG 2 cut(s) 443, 683
AflII CTTAAG 1 cut(s) 506
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 7 cut(s) 25, 64, 278, 467, 517, 610, 785
Alw21I GWGCWC 3 cut(s) 487, 742, 874
Alw26I GTCTC 2 cut(s) 9, 830
Ama87I CYCGRG 1 cut(s) 873
AoxI GGCC 2 cut(s) 157, 812
ApoI RAATTY 6 cut(s) 64, 177, 248, 379, 407, 572
AspLEI GCGC 2 cut(s) 129, 142
AvaI CYCGRG 1 cut(s) 873
BaeGI GKGCMC 1 cut(s) 691
BalI TGGCCA 1 cut(s) 159
BanII GRGCYC 1 cut(s) 874
Bbv12I GWGCWC 3 cut(s) 487, 742, 874
BccI CCATC 2 cut(s) 772, 796
BcoDI GTCTC 2 cut(s) 9, 830
BfmI CTRYAG 1 cut(s) 705
BfrI CTTAAG 1 cut(s) 506
BglI GCCNNNNNGGC 1 cut(s) 920
BglII AGATCT 1 cut(s) 746
BlpI GCTNAGC 2 cut(s) 894, 927
BmeT110I CYCGRG 1 cut(s) 873
BmiI GGNNCC 1 cut(s) 267
BmsI GCATC 2 cut(s) 335, 911
BpmI CTGGAG 1 cut(s) 513
Bpu1102I GCTNAGC 2 cut(s) 894, 927
BpuEI CTTGAG 1 cut(s) 624
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 683
Bse1I ACTGG 1 cut(s) 815
Bse3DI GCAATG 1 cut(s) 882
BseLI CCNNNNNNNGG 2 cut(s) 443, 683
BseMI GCAATG 1 cut(s) 882
BseMII CTCAG 4 cut(s) 363, 417, 885, 918
BseNI ACTGG 1 cut(s) 815
BseSI GKGCMC 1 cut(s) 691
BseYI CCCAGC 1 cut(s) 598
BsgI GTGCAG 2 cut(s) 131, 615
BshFI GGCC 2 cut(s) 159, 814
BsiHKAI GWGCWC 3 cut(s) 487, 742, 874
BsiHKCI CYCGRG 1 cut(s) 873
BslI CCNNNNNNNGG 2 cut(s) 443, 683
BsmAI GTCTC 2 cut(s) 9, 830
BsnI GGCC 2 cut(s) 159, 814
BsoBI CYCGRG 1 cut(s) 873
Bsp1286I GDGCHC 4 cut(s) 487, 691, 742, 874
Bsp143I GATC 3 cut(s) 372, 592, 746
Bsp1720I GCTNAGC 2 cut(s) 894, 927
BspACI CCGC 3 cut(s) 336, 569, 648
BspANI GGCC 2 cut(s) 159, 814
BspCNI CTCAG 4 cut(s) 362, 418, 886, 919
BspLI GGNNCC 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 709
BspQI GCTCTTC 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 882
BsrI ACTGG 1 cut(s) 815
BssMI GATC 3 cut(s) 372, 592, 746
Bst6I CTCTTC 1 cut(s) 26
BstAFI CTTAAG 1 cut(s) 506
BstAPI GCANNNNNTGC 3 cut(s) 48, 148, 908
BstC8I GCNNGC 4 cut(s) 153, 157, 705, 900
BstDEI CTNAG 4 cut(s) 349, 426, 894, 927
BstHHI GCGC 2 cut(s) 129, 142
BstKTI GATC 3 cut(s) 375, 595, 749
BstMAI GTCTC 2 cut(s) 9, 830
BstMBI GATC 3 cut(s) 372, 592, 746
BstMWI GCNNNNNNNGC 8 cut(s) 39, 48, 148, 878, 884, 899, 908, 920
BstNSI RCATGY 3 cut(s) 60, 194, 617
BstSFI CTRYAG 1 cut(s) 705
BstSLI GKGCMC 1 cut(s) 691
BstX2I RGATCY 1 cut(s) 746
BstYI RGATCY 1 cut(s) 746
BsuRI GGCC 2 cut(s) 159, 814
BtsIMutI CAGTG 1 cut(s) 561
Cac8I GCNNGC 4 cut(s) 153, 157, 705, 900
CfoI GCGC 2 cut(s) 129, 142
CseI GACGC 1 cut(s) 411
Csp6I GTAC 2 cut(s) 254, 535
CviAII CATG 4 cut(s) 57, 191, 587, 614
CviQI GTAC 2 cut(s) 254, 535
DdeI CTNAG 4 cut(s) 349, 426, 894, 927
DpnI GATC 3 cut(s) 374, 594, 748
DpnII GATC 3 cut(s) 372, 592, 746
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 872
Eco24I GRGCYC 1 cut(s) 874
Eco32I GATATC 1 cut(s) 859
Eco53kI GAGCTC 1 cut(s) 872
Eco57I CTGAAG 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 873
EcoICRI GAGCTC 1 cut(s) 872
EcoRI GAATTC 2 cut(s) 177, 572
EcoRV GATATC 1 cut(s) 859
EcoT38I GRGCYC 1 cut(s) 874
FaeI CATG 4 cut(s) 60, 194, 590, 617
FatI CATG 4 cut(s) 56, 190, 586, 613
FauI CCCGC 1 cut(s) 655
FauNDI CATATG 1 cut(s) 123
FriOI GRGCYC 1 cut(s) 874
FspI TGCGCA 2 cut(s) 128, 141
GlaI GCGC 2 cut(s) 128, 141
GsaI CCCAGC 1 cut(s) 602
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 2 cut(s) 159, 814
HgaI GACGC 1 cut(s) 411
HhaI GCGC 2 cut(s) 129, 142
Hin1II CATG 4 cut(s) 60, 194, 590, 617
Hin6I GCGC 2 cut(s) 127, 140
HinP1I GCGC 2 cut(s) 127, 140
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 3 cut(s) 440, 551, 781
Hpy166II GTNNAC 2 cut(s) 387, 451
Hpy188I TCNGA 5 cut(s) 285, 352, 427, 559, 856
Hpy188III TCNNGA 3 cut(s) 359, 437, 492
Hpy8I GTNNAC 2 cut(s) 387, 451
Hpy99I CGWCG 1 cut(s) 921
HpyAV CCTTC 4 cut(s) 397, 511, 645, 687
HpyF10VI GCNNNNNNNGC 8 cut(s) 39, 48, 148, 878, 884, 899, 908, 920
HpyF3I CTNAG 4 cut(s) 349, 426, 894, 927
Hsp92II CATG 4 cut(s) 60, 194, 590, 617
HspAI GCGC 2 cut(s) 127, 140
Kzo9I GATC 3 cut(s) 372, 592, 746
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 5 cut(s) 187, 235, 490, 649, 745
LweI GCATC 2 cut(s) 335, 911
MaeIII GTNAC 1 cut(s) 658
MalI GATC 3 cut(s) 374, 594, 748
MboI GATC 3 cut(s) 372, 592, 746
MboII GAAGA 2 cut(s) 13, 546
MflI RGATCY 1 cut(s) 746
MhlI GDGCHC 4 cut(s) 487, 691, 742, 874
MlsI TGGCCA 1 cut(s) 159
MluNI TGGCCA 1 cut(s) 159
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 6 cut(s) 23, 279, 490, 735, 743, 898
Mox20I TGGCCA 1 cut(s) 159
MscI TGGCCA 1 cut(s) 159
MseI TTAA 7 cut(s) 11, 84, 365, 507, 714, 752, 936
MslI CAYNNNNRTG 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 419
MspCI CTTAAG 1 cut(s) 506
MwoI GCNNNNNNNGC 8 cut(s) 39, 48, 148, 878, 884, 899, 908, 920
NdeI CATATG 1 cut(s) 123
NdeII GATC 3 cut(s) 372, 592, 746
NlaIII CATG 4 cut(s) 60, 194, 590, 617
NlaIV GGNNCC 1 cut(s) 267
NsbI TGCGCA 2 cut(s) 128, 141
NspI RCATGY 3 cut(s) 60, 194, 617
PaeR7I CTCGAG 1 cut(s) 873
PciI ACATGT 1 cut(s) 56
PciSI GCTCTTC 1 cut(s) 26
PfeI GAWTC 3 cut(s) 440, 551, 781
PscI ACATGT 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 874
PspFI CCCAGC 1 cut(s) 598
PspN4I GGNNCC 1 cut(s) 267
PspXI VCTCGAGB 1 cut(s) 873
PstI CTGCAG 1 cut(s) 709
PsuI RGATCY 1 cut(s) 746
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 2 cut(s) 255, 536
RsaNI GTAC 2 cut(s) 254, 535
RseI CAYNNNNRTG 1 cut(s) 618
SacI GAGCTC 1 cut(s) 874
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 7 cut(s) 11, 84, 365, 507, 714, 752, 936
Sau3AI GATC 3 cut(s) 372, 592, 746
SduI GDGCHC 4 cut(s) 487, 691, 742, 874
SfaNI GCATC 2 cut(s) 335, 911
SfcI CTRYAG 1 cut(s) 705
Sfr274I CTCGAG 1 cut(s) 873
SlaI CTCGAG 1 cut(s) 873
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 3 cut(s) 506, 639, 873
SmoI CTYRAG 3 cut(s) 506, 639, 873
SsiI CCGC 3 cut(s) 336, 569, 648
SstI GAGCTC 1 cut(s) 874
TaqI TCGA 3 cut(s) 175, 320, 874
TatI WGTACW 2 cut(s) 253, 534
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 3 cut(s) 440, 551, 781
Tru1I TTAA 7 cut(s) 11, 84, 365, 507, 714, 752, 936
Tru9I TTAA 7 cut(s) 11, 84, 365, 507, 714, 752, 936
TscAI CASTG 1 cut(s) 568
TspGWI ACGGA 3 cut(s) 314, 477, 725
TspRI CASTG 1 cut(s) 568
Vha464I CTTAAG 1 cut(s) 506
XapI RAATTY 6 cut(s) 64, 177, 248, 379, 407, 572
XceI RCATGY 3 cut(s) 60, 194, 617
XhoI CTCGAG 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.