AT1G27990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009383
Physical Location & Seq
Reverse (-)
9752742 .. 9753952
1211 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27990.1

Sequence Viewer

Length: 816 bp
ATGTCAGGCGTGTCTCTTGCGGTTGGTCCAAGAACCGACGTGGACAAAACGGCGTCATCCAGTGAGAAGGGACGGTGGTCGGGGATGACGGCGATAGGAGGTGGCAGTGGAGGTTTGATGGGGTCGTTAAGGGTTATAGAGCTACAGCTAGTCGCGTTCATACTAGTTTTCTCAGCAAGCGGTCTCGTACCAATACTTGACATGCTTTTTCCAGCGTTTGCGTCTATCTATATAATCGCCCTCTCGCGTTTAGCTTTCCCTTCTCACGGCGTTTCCACCGCTTCCCCCGAAGTCTTCCGCGGCAGCAAACTCTTCAGGTTGTACGTGATTTCGGGGACAACGATTGGTCTGTTCTTGCCTCTAGCTTACGTATTAGGTGGTTTTGCAAGAGGAGATGATCATGCGGTAAGATCAGCAACACCACACTTGTTTCTACTGTCGTGTCAAATCCTGACAGAGAATGTAATAAGTGGCCTTTCTCTGTTCTCGCCACCGGTAAGAGCTCTCGTCCCATTGCTCTACACAGTCTGGAGAATCTTTGTCATCATTGGTTGGTCTAAAGATGTTTGGTTCAACAAGTCTTTACCAATCAATGCTACACCCAATGTGGTTACATGGTTCTGGTTTGGAAGGTATTTAGCACTAGCAAACTTAGGCTACTTCGGAGTGAATCTACTCTGTTTCTTGATCCCTAGGTTCCTGCCTCGTGCTTTCGAGCAATACTTCAGAGAGAGAGACGAGATCTTGGCGAAAAGCCAAGAAGACAAGCCAGTTCAAGTTCCTAGATCAAAACCTTCTAACCACAAATCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

271

Amino Acids

29.76

Weight (kDa)

9.69

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 44 - 242 7.2e-78 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 155, 247, 300
AciI CCGC 6 cut(s) 20, 180, 279, 298, 300, 404
AclWI GGATC 1 cut(s) 682
AcuI CTGAAG 2 cut(s) 298, 709
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 2 cut(s) 189, 323
AfiI CCNNNNNNNGG 1 cut(s) 266
AgeI ACCGGT 1 cut(s) 493
AgsI TTSAA 2 cut(s) 574, 776
AhlI ACTAGT 1 cut(s) 163
AjiI CACGTC 1 cut(s) 40
AluBI AGCT 5 cut(s) 142, 148, 254, 365, 503
AluI AGCT 5 cut(s) 142, 148, 254, 365, 503
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 3 cut(s) 18, 188, 729
AlwI GGATC 1 cut(s) 682
AoxI GGCC 1 cut(s) 472
ApeKI GCWGC 1 cut(s) 303
AsiGI ACCGGT 1 cut(s) 493
AspA2I CCTAGG 1 cut(s) 692
AspS9I GGNCC 1 cut(s) 26
AvaII GGWCC 1 cut(s) 26
AvrII CCTAGG 1 cut(s) 692
BanII GRGCYC 1 cut(s) 505
BauI CACGAG 1 cut(s) 705
BbsI GAAGAC 2 cut(s) 286, 768
Bbv12I GWGCWC 1 cut(s) 505
BbvI GCAGC 1 cut(s) 315
BccI CCATC 1 cut(s) 112
BceAI ACGGC 3 cut(s) 66, 105, 283
BclI TGATCA 1 cut(s) 397
BcoDI GTCTC 3 cut(s) 18, 188, 729
BcuI ACTAGT 1 cut(s) 163
BfaI CTAG 6 cut(s) 149, 164, 362, 644, 693, 783
BfmI CTRYAG 1 cut(s) 143
BglII AGATCT 1 cut(s) 741
BisI GCNGC 2 cut(s) 301, 304
BlnI CCTAGG 1 cut(s) 692
BlsI GCNGC 2 cut(s) 302, 305
Bme18I GGWCC 1 cut(s) 26
BmgBI CACGTC 1 cut(s) 40
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 698
BoxI GACNNNNGTC 1 cut(s) 76
BpiI GAAGAC 2 cut(s) 286, 768
BpmI CTGGAG 1 cut(s) 550
BsaAI YACGTR 2 cut(s) 325, 370
BsaHI GRCGYC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 298, 692
BsaWI WCCGGW 1 cut(s) 493
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse118I RCCGGY 1 cut(s) 493
Bse1I ACTGG 2 cut(s) 60, 770
Bse3DI GCAATG 1 cut(s) 512
BseDI CCNNGG 2 cut(s) 298, 692
BseGI GGATG 2 cut(s) 56, 90
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMI GCAATG 1 cut(s) 512
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 2 cut(s) 60, 770
BseRI GAGGAG 1 cut(s) 405
BseXI GCAGC 1 cut(s) 315
Bsh1236I CGCG 3 cut(s) 155, 247, 300
BshFI GGCC 1 cut(s) 474
BshTI ACCGGT 1 cut(s) 493
BsiHKAI GWGCWC 1 cut(s) 505
BsiSI CCGG 1 cut(s) 494
BslFI GGGAC 3 cut(s) 84, 349, 494
BslI CCNNNNNNNGG 1 cut(s) 266
BsmAI GTCTC 3 cut(s) 18, 188, 729
BsmBI CGTCTC 1 cut(s) 729
BsmFI GGGAC 3 cut(s) 84, 349, 494
BsnI GGCC 1 cut(s) 474
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 505
Bsp143I GATC 5 cut(s) 397, 410, 687, 741, 785
BspACI CCGC 6 cut(s) 20, 180, 279, 298, 300, 404
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 1 cut(s) 185
BspFNI CGCG 3 cut(s) 155, 247, 300
BspLI GGNNCC 1 cut(s) 698
BspPI GGATC 1 cut(s) 682
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 512
BsrFI RCCGGY 1 cut(s) 493
BsrI ACTGG 2 cut(s) 60, 770
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 2 cut(s) 298, 692
BssMI GATC 5 cut(s) 397, 410, 687, 741, 785
BssNI GRCGYC 1 cut(s) 53
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 1 cut(s) 692
Bst2BI CACGAG 1 cut(s) 705
Bst4CI ACNGT 3 cut(s) 75, 438, 526
Bst6I CTCTTC 1 cut(s) 317
BstACI GRCGYC 1 cut(s) 53
BstBAI YACGTR 2 cut(s) 325, 370
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 2 cut(s) 172, 652
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 2 cut(s) 56, 90
BstFNI CGCG 3 cut(s) 155, 247, 300
BstKTI GATC 5 cut(s) 400, 413, 690, 744, 788
BstMAI GTCTC 3 cut(s) 18, 188, 729
BstMBI GATC 5 cut(s) 397, 410, 687, 741, 785
BstNSI RCATGY 1 cut(s) 205
BstPAI GACNNNNGTC 1 cut(s) 76
BstSFI CTRYAG 1 cut(s) 143
BstSNI TACGTA 1 cut(s) 370
BstUI CGCG 3 cut(s) 155, 247, 300
BstV1I GCAGC 1 cut(s) 315
BstV2I GAAGAC 2 cut(s) 286, 768
BstX2I RGATCY 1 cut(s) 741
BstYI RGATCY 1 cut(s) 741
BsuRI GGCC 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 298
BtrI CACGTC 1 cut(s) 40
BtsCI GGATG 2 cut(s) 56, 90
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 2 cut(s) 67, 112
Cac8I GCNNGC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 493
Cfr13I GGNCC 1 cut(s) 26
Cfr42I CCGCGG 1 cut(s) 301
CseI GACGC 2 cut(s) 42, 210
Csp6I GTAC 2 cut(s) 188, 322
CspAI ACCGGT 1 cut(s) 493
CviAII CATG 3 cut(s) 202, 401, 615
CviJI RGCY 9 cut(s) 142, 148, 254, 365, 474, 503, 657, 756, 769
CviKI_1 RGCY 9 cut(s) 142, 148, 254, 365, 474, 503, 657, 756, 769
CviQI GTAC 2 cut(s) 188, 322
DdeI CTNAG 2 cut(s) 172, 652
DpnI GATC 5 cut(s) 399, 412, 689, 743, 787
DpnII GATC 5 cut(s) 397, 410, 687, 741, 785
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Ecl136II GAGCTC 1 cut(s) 503
Eco105I TACGTA 1 cut(s) 370
Eco130I CCWWGG 1 cut(s) 692
Eco24I GRGCYC 1 cut(s) 505
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 503
Eco57I CTGAAG 2 cut(s) 298, 709
EcoICRI GAGCTC 1 cut(s) 503
EcoT14I CCWWGG 1 cut(s) 692
EcoT38I GRGCYC 1 cut(s) 505
ErhI CCWWGG 1 cut(s) 692
Esp3I CGTCTC 1 cut(s) 729
FaeI CATG 3 cut(s) 205, 404, 618
FaiI YATR 7 cut(s) 137, 161, 203, 231, 233, 402, 616
FaqI GGGAC 3 cut(s) 84, 349, 494
FatI CATG 3 cut(s) 201, 400, 614
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 301, 304
FokI GGATG 2 cut(s) 43, 97
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 2 cut(s) 301, 304
FspBI CTAG 6 cut(s) 149, 164, 362, 644, 693, 783
GluI GCNGC 2 cut(s) 301, 304
GsuI CTGGAG 1 cut(s) 550
HaeIII GGCC 1 cut(s) 474
HapII CCGG 1 cut(s) 494
HgaI GACGC 2 cut(s) 42, 210
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 3 cut(s) 205, 404, 618
HinfI GANTC 2 cut(s) 534, 670
HpaII CCGG 1 cut(s) 494
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 3 cut(s) 665, 728, 811
Hpy188III TCNNGA 3 cut(s) 451, 529, 685
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 4 cut(s) 61, 270, 624, 804
HpyCH4III ACNGT 3 cut(s) 75, 438, 526
HpyCH4IV ACGT 3 cut(s) 39, 324, 369
HpyCH4V TGCA 1 cut(s) 386
HpyF3I CTNAG 2 cut(s) 172, 652
HpySE526I ACGT 3 cut(s) 39, 324, 369
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 3 cut(s) 205, 404, 618
Ksp22I TGATCA 1 cut(s) 397
KspI CCGCGG 1 cut(s) 301
Kzo9I GATC 5 cut(s) 397, 410, 687, 741, 785
LpnPI CCDG 9 cut(s) 73, 225, 301, 464, 507, 514, 607, 713, 783
Lsp1109I GCAGC 1 cut(s) 315
MaeI CTAG 6 cut(s) 149, 164, 362, 644, 693, 783
MaeII ACGT 3 cut(s) 39, 324, 369
MaeIII GTNAC 1 cut(s) 610
MalI GATC 5 cut(s) 399, 412, 689, 743, 787
MboI GATC 5 cut(s) 397, 410, 687, 741, 785
MboII GAAGA 3 cut(s) 286, 304, 773
MflI RGATCY 1 cut(s) 741
MhlI GDGCHC 1 cut(s) 505
MnlI CCTC 6 cut(s) 92, 104, 251, 369, 383, 714
MseI TTAA 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 300
MspI CCGG 1 cut(s) 494
MvnI CGCG 3 cut(s) 155, 247, 300
NdeII GATC 5 cut(s) 397, 410, 687, 741, 785
NlaIII CATG 3 cut(s) 205, 404, 618
NlaIV GGNNCC 1 cut(s) 698
NspI RCATGY 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 338
PfeI GAWTC 2 cut(s) 534, 670
PinAI ACCGGT 1 cut(s) 493
PkrI GCNGC 2 cut(s) 302, 305
Ppu21I YACGTR 2 cut(s) 325, 370
PshAI GACNNNNGTC 1 cut(s) 76
Psp124BI GAGCTC 1 cut(s) 505
PspN4I GGNNCC 1 cut(s) 698
PspPI GGNCC 1 cut(s) 26
PsuI RGATCY 1 cut(s) 741
RsaI GTAC 2 cut(s) 189, 323
RsaNI GTAC 2 cut(s) 188, 322
SacI GAGCTC 1 cut(s) 505
SacII CCGCGG 1 cut(s) 301
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 2 cut(s) 301, 304
Sau3AI GATC 5 cut(s) 397, 410, 687, 741, 785
Sau96I GGNCC 1 cut(s) 26
SduI GDGCHC 1 cut(s) 505
SfcI CTRYAG 1 cut(s) 143
Sfr303I CCGCGG 1 cut(s) 301
SgrBI CCGCGG 1 cut(s) 301
SinI GGWCC 1 cut(s) 26
SnaBI TACGTA 1 cut(s) 370
SpeI ACTAGT 1 cut(s) 163
SsiI CCGC 6 cut(s) 20, 180, 279, 298, 300, 404
SspMI CTAG 6 cut(s) 149, 164, 362, 644, 693, 783
SstI GAGCTC 1 cut(s) 505
StyI CCWWGG 1 cut(s) 692
TaaI ACNGT 3 cut(s) 75, 438, 526
TaiI ACGT 3 cut(s) 42, 327, 372
TaqI TCGA 1 cut(s) 714
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 2 cut(s) 534, 670
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TscAI CASTG 2 cut(s) 67, 112
TseI GCWGC 1 cut(s) 303
TspDTI ATGAA 1 cut(s) 148
TspRI CASTG 2 cut(s) 67, 112
VpaK11BI GGWCC 1 cut(s) 26
XceI RCATGY 1 cut(s) 205
XmaJI CCTAGG 1 cut(s) 692
XspI CTAG 6 cut(s) 149, 164, 362, 644, 693, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.