Rorug04G0238300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009383
Physical Location & Seq
Reverse (-)
40518881 .. 40520308
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0238300.1

Sequence Viewer

Length: 1428 bp
ATGGCCAGAGCTTCATCTTCAATCTTAATATTCCTCCGCCAAAACCCTCGTGCTCTCAAAAAACCCAATACCCAAATCCGATACCTTACCGTAGAAACCAAAGATTGTGACTTTACAGAAGTTGCCCAACAGATTTGTAGGATCATTAGAACAAAACCCAGATGGGAGAACACTCTGTCCTCTGAGTACCCTTCTTTAGATTTCTCTGATCCTCGGTTTATCCGTGAGGTTTTGAAGCAGCAGAGCAGTGTGCTCCTTTCGGTTCGGTTCTTCTTGTGGTTGAGCTCTCGAAAAGGGTTTTCGCCAGACCCCATTTCGTGTAATGCGGTTTTCAATGCTCTTGTGGAGGCTAAGGCCTGCAGTGCAGCACAATCTTTTCTTAAGTCCACGGGTTTTAGCCCCGAGCCGGTTTCATTGGAAAGTTATGCTCGGTGTCTTTGGGAGGCTGGGAGGATTAAGGAAGCCTGTGATGTGTTTGAGAGGTTGAAAGGGGCTGGAGTGTGCCCGTCGATTGGGACTTGGAATGCGGCTTTGTCGGGGTGCCTGAAGGCTAAGAGGACAGATATGGTTTGGAAGTTGTATCAAGAAATGATGGAATATGGTGTTGTAGCTGATGTTGAGACTGTTGGGTGTCTCATTCGAGGATTTTGTGATGATAATGAGGTATTCAAAGGTTATGGACTTCTTACTCAGGTTTTGGAAGATGGAGTAGTCCCTGGAAAAGCTGCTTTCAATCGGTTGATATCTGAGTTTTGTAAGGAGAAGGAATATACTAAAGTGTCTGACCTCCTCCATACAATGATAGTGATGAACTGTGCTCCCGATATTTTTACTTATCAGGAGGTAATCAATTGGTTGTGCAAGAAAGGAAGGCCATATGAGGGTTTTCGGATTTTCAATGATCTTAAGTATAGAGGCTATGCTCCTGATATTGTCATGTACACAACCATGATTCATGGTCTTTGTACGATGGGTTGGCTTGGAGAAGCTAGGAAGCTATGGTTTGAGATGATTGATAAGGGATTTCATCCAAGCAAGTACACTTGCAACACCCTAATTCATGGGTTTTGTAAGAGTGGAATTTTAGAACAGGCCCAGATGTGCAGGGAGATGTGTGATAAGGGATTTCGTCCAAGCAAGTACACTAACAATACCATGATTCATGGGTTTTGTAAGAGTGGAAATTTAGAACAGGCCTGGATCTTGTGCAAGGAGATGGGTGATAAGGGATTTCGTCCAAGCAAGTACATTTACGATATCATGATTCATGGGTTTTGTAAGAGTGGAAATTTCAAACAGGCCAGGATCATTTGCAAGGAGATGTGTGATAAAGGTTATATGGAAACCACAATCAATCGCAACGCAATGATGACAGGGCTTTGTTTACATGGAAGGACAGGTCAGGCTTATAAGCTGTTTAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

475

Amino Acids

54.22

Weight (kDa)

8.91

Isoelectric Point (pI)

30.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 218 3.2e-11 PPR repeat family
PPR_2 PF13041 242 - 279 9.1e-07 PPR repeat family
PPR_2 PF13041 282 - 322 3.6e-10 PPR repeat family
PPR_1 PF12854 305 - 337 8.4e-09 PPR repeat
PPR_2 PF13041 309 - 358 6.1e-15 PPR repeat family
PPR PF01535 312 - 342 2.6e-07 PPR repeat
PPR_2 PF13041 348 - 392 7.8e-11 PPR repeat family
PPR_1 PF12854 374 - 406 2e-07 PPR repeat
PPR_2 PF13041 384 - 427 2.3e-13 PPR repeat family
PPR PF01535 384 - 411 9.5e-06 PPR repeat
PPR_1 PF12854 409 - 441 6.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1410
AccB1I GGYRCC 1 cut(s) 540
AciI CCGC 3 cut(s) 37, 326, 527
AclWI GGATC 4 cut(s) 149, 203, 1208, 1313
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 1080, 1183, 1288
AcuI CTGAAG 1 cut(s) 566
AfaI GTAC 6 cut(s) 188, 941, 967, 1040, 1142, 1247
AfiI CCNNNNNNNGG 3 cut(s) 406, 512, 881
AflII CTTAAG 2 cut(s) 380, 905
AgsI TTSAA 8 cut(s) 21, 235, 334, 487, 670, 733, 898, 1294
AjnI CCWGG 3 cut(s) 715, 1196, 1301
AloI GAACNNNNNNTCC 2 cut(s) 161, 193
AluBI AGCT 7 cut(s) 11, 285, 611, 725, 989, 997, 1414
AluI AGCT 7 cut(s) 11, 285, 611, 725, 989, 997, 1414
Alw21I GWGCWC 4 cut(s) 55, 255, 287, 820
Alw26I GTCTC 2 cut(s) 614, 638
AlwI GGATC 4 cut(s) 149, 203, 1208, 1313
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 6 cut(s) 3, 354, 872, 1092, 1194, 1299
ApeKI GCWGC 3 cut(s) 238, 365, 725
ApoI RAATTY 3 cut(s) 1080, 1183, 1288
AspS9I GGNCC 1 cut(s) 1093
AsuHPI GGTGA 1 cut(s) 1232
AvaI CYCGRG 1 cut(s) 401
BaeGI GKGCMC 1 cut(s) 506
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 540
BanII GRGCYC 1 cut(s) 287
BauI CACGAG 1 cut(s) 48
Bbv12I GWGCWC 4 cut(s) 55, 255, 287, 820
BbvI GCAGC 3 cut(s) 250, 377, 712
BccI CCATC 5 cut(s) 156, 586, 698, 964, 1210
BciT130I CCWGG 3 cut(s) 717, 1198, 1303
BcoDI GTCTC 2 cut(s) 614, 638
BfaI CTAG 1 cut(s) 990
BfmI CTRYAG 1 cut(s) 358
BfrI CTTAAG 2 cut(s) 380, 905
BisI GCNGC 4 cut(s) 239, 366, 528, 726
BlsI GCNGC 4 cut(s) 240, 367, 529, 727
Bme1390I CCNGG 3 cut(s) 717, 1198, 1303
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 1 cut(s) 1093
BmiI GGNNCC 1 cut(s) 542
BmrFI CCNGG 3 cut(s) 717, 1198, 1303
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 351
BsaJI CCNNGG 3 cut(s) 212, 387, 715
Bsc4I CCNNNNNNNGG 3 cut(s) 406, 512, 881
Bse118I RCCGGY 1 cut(s) 406
Bse3DI GCAATG 1 cut(s) 1371
BseBI CCWGG 3 cut(s) 717, 1198, 1303
BseDI CCNNGG 3 cut(s) 212, 387, 715
BseGI GGATG 1 cut(s) 1027
BseLI CCNNNNNNNGG 3 cut(s) 406, 512, 881
BseMI GCAATG 1 cut(s) 1371
BseMII CTCAG 3 cut(s) 174, 704, 738
BseRI GAGGAG 1 cut(s) 779
BseSI GKGCMC 1 cut(s) 506
BseXI GCAGC 3 cut(s) 250, 377, 712
BseYI CCCAGC 1 cut(s) 446
BsgI GTGCAG 2 cut(s) 384, 1123
BshFI GGCC 6 cut(s) 5, 356, 874, 1094, 1196, 1301
BshNI GGYRCC 1 cut(s) 540
BsiHKAI GWGCWC 4 cut(s) 55, 255, 287, 820
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 1 cut(s) 407
BslFI GGGAC 2 cut(s) 529, 698
BslI CCNNNNNNNGG 3 cut(s) 406, 512, 881
BsmAI GTCTC 2 cut(s) 614, 638
BsmFI GGGAC 2 cut(s) 529, 698
BsmI GAATGC 1 cut(s) 529
BsnI GGCC 6 cut(s) 5, 356, 874, 1094, 1196, 1301
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 5 cut(s) 55, 255, 287, 506, 820
Bsp1407I TGTACA 1 cut(s) 939
Bsp143I GATC 5 cut(s) 141, 208, 901, 1200, 1305
BspACI CCGC 3 cut(s) 37, 326, 527
BspANI GGCC 6 cut(s) 5, 356, 874, 1094, 1196, 1301
BspCNI CTCAG 3 cut(s) 175, 703, 739
BspHI TCATGA 1 cut(s) 1260
BspLI GGNNCC 1 cut(s) 542
BspMAI CTGCAG 1 cut(s) 362
BspPI GGATC 4 cut(s) 149, 203, 1208, 1313
BspT107I GGYRCC 1 cut(s) 540
BspTI CTTAAG 2 cut(s) 380, 905
BsrDI GCAATG 1 cut(s) 1371
BsrFI RCCGGY 1 cut(s) 406
BsrGI TGTACA 1 cut(s) 939
BssAI RCCGGY 1 cut(s) 406
BssECI CCNNGG 3 cut(s) 212, 387, 715
BssMI GATC 5 cut(s) 141, 208, 901, 1200, 1305
BssSI CACGAG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 48
Bst2UI CCWGG 3 cut(s) 717, 1198, 1303
Bst4CI ACNGT 3 cut(s) 91, 625, 815
BstAFI CTTAAG 2 cut(s) 380, 905
BstAUI TGTACA 1 cut(s) 939
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 5 cut(s) 183, 351, 552, 690, 747
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 1 cut(s) 1027
BstKTI GATC 5 cut(s) 144, 211, 904, 1203, 1308
BstMAI GTCTC 2 cut(s) 614, 638
BstMBI GATC 5 cut(s) 141, 208, 901, 1200, 1305
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 3 cut(s) 717, 1198, 1303
BstSCI CCNGG 3 cut(s) 715, 1196, 1301
BstSFI CTRYAG 1 cut(s) 358
BstSLI GKGCMC 1 cut(s) 506
BstV1I GCAGC 3 cut(s) 250, 377, 712
BstX2I RGATCY 1 cut(s) 1200
BstYI RGATCY 1 cut(s) 1200
BsuRI GGCC 6 cut(s) 5, 356, 874, 1094, 1196, 1301
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 1 cut(s) 1027
BtsI GCAGTG 2 cut(s) 253, 367
BtsIMutI CAGTG 2 cut(s) 253, 367
Cac8I GCNNGC 1 cut(s) 358
CciI TCATGA 1 cut(s) 1260
Cfr10I RCCGGY 1 cut(s) 406
Cfr13I GGNCC 1 cut(s) 1093
Csp6I GTAC 6 cut(s) 187, 940, 966, 1039, 1141, 1246
CviAII CATG 9 cut(s) 937, 949, 956, 1061, 1156, 1163, 1261, 1268, 1388
CviQI GTAC 6 cut(s) 187, 940, 966, 1039, 1141, 1246
DdeI CTNAG 5 cut(s) 183, 351, 552, 690, 747
DpnI GATC 5 cut(s) 143, 210, 903, 1202, 1307
DpnII GATC 5 cut(s) 141, 208, 901, 1200, 1305
DraI TTTAAA 1 cut(s) 1420
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 285
Eco147I AGGCCT 2 cut(s) 356, 1196
Eco24I GRGCYC 1 cut(s) 287
Eco32I GATATC 2 cut(s) 744, 1258
Eco53kI GAGCTC 1 cut(s) 285
Eco57I CTGAAG 1 cut(s) 566
Eco88I CYCGRG 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 285
EcoRII CCWGG 3 cut(s) 715, 1196, 1301
EcoRV GATATC 2 cut(s) 744, 1258
EcoT38I GRGCYC 1 cut(s) 287
FaeI CATG 9 cut(s) 940, 952, 959, 1064, 1159, 1166, 1264, 1271, 1391
FaqI GGGAC 2 cut(s) 529, 698
FatI CATG 9 cut(s) 936, 948, 955, 1060, 1155, 1162, 1260, 1267, 1387
FauNDI CATATG 1 cut(s) 877
Fnu4HI GCNGC 4 cut(s) 239, 366, 528, 726
FokI GGATG 1 cut(s) 1014
FriOI GRGCYC 1 cut(s) 287
Fsp4HI GCNGC 4 cut(s) 239, 366, 528, 726
FspBI CTAG 1 cut(s) 990
GluI GCNGC 4 cut(s) 239, 366, 528, 726
GsaI CCCAGC 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 6 cut(s) 5, 356, 874, 1094, 1196, 1301
HapII CCGG 1 cut(s) 407
Hin1II CATG 9 cut(s) 940, 952, 959, 1064, 1159, 1166, 1264, 1271, 1391
HinfI GANTC 3 cut(s) 952, 1159, 1264
HpaII CCGG 1 cut(s) 407
HphI GGTGA 1 cut(s) 1232
Hpy166II GTNNAC 5 cut(s) 387, 942, 1041, 1143, 1385
Hpy188I TCNGA 6 cut(s) 80, 184, 208, 748, 784, 891
Hpy188III TCNNGA 6 cut(s) 288, 584, 821, 839, 926, 1261
Hpy8I GTNNAC 5 cut(s) 387, 942, 1041, 1143, 1385
Hpy99I CGWCG 1 cut(s) 511
HpyAV CCTTC 5 cut(s) 201, 541, 757, 864, 1386
HpyCH4III ACNGT 3 cut(s) 91, 625, 815
HpyCH4V TGCA 7 cut(s) 360, 365, 861, 1047, 1104, 1209, 1314
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 5 cut(s) 183, 351, 552, 690, 747
Hsp92II CATG 9 cut(s) 940, 952, 959, 1064, 1159, 1166, 1264, 1271, 1391
Kzo9I GATC 5 cut(s) 141, 208, 901, 1200, 1305
LmnI GCTCC 3 cut(s) 258, 823, 928
Lsp1109I GCAGC 3 cut(s) 250, 377, 712
MaeI CTAG 1 cut(s) 990
MaeIII GTNAC 1 cut(s) 107
MalI GATC 5 cut(s) 143, 210, 903, 1202, 1307
MboI GATC 5 cut(s) 141, 208, 901, 1200, 1305
MboII GAAGA 3 cut(s) 9, 262, 713
MfeI CAATTG 1 cut(s) 850
MflI RGATCY 1 cut(s) 1200
MhlI GDGCHC 5 cut(s) 55, 255, 287, 506, 820
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 850, 1056, 1080, 1183, 1288
MluNI TGGCCA 1 cut(s) 5
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 26, 381, 456, 906, 1419
MslI CAYNNNNRTG 1 cut(s) 947
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 2 cut(s) 380, 905
MspI CCGG 1 cut(s) 407
MspR9I CCNGG 3 cut(s) 717, 1198, 1303
MunI CAATTG 1 cut(s) 850
Mva1269I GAATGC 1 cut(s) 529
MvaI CCWGG 3 cut(s) 717, 1198, 1303
MwoI GCNNNNNNNGC 1 cut(s) 362
NdeI CATATG 1 cut(s) 877
NdeII GATC 5 cut(s) 141, 208, 901, 1200, 1305
NlaIII CATG 9 cut(s) 940, 952, 959, 1064, 1159, 1166, 1264, 1271, 1391
NlaIV GGNNCC 1 cut(s) 542
NmuCI GTSAC 1 cut(s) 107
PagI TCATGA 1 cut(s) 1260
PceI AGGCCT 2 cut(s) 356, 1196
PcsI WCGNNNNNNNCGW 1 cut(s) 220
PctI GAATGC 1 cut(s) 529
PfeI GAWTC 3 cut(s) 952, 1159, 1264
PkrI GCNGC 4 cut(s) 240, 367, 529, 727
PsiI TTATAA 1 cut(s) 1410
Psp124BI GAGCTC 1 cut(s) 287
Psp6I CCWGG 3 cut(s) 715, 1196, 1301
PspFI CCCAGC 1 cut(s) 446
PspGI CCWGG 3 cut(s) 715, 1196, 1301
PspN4I GGNNCC 1 cut(s) 542
PspPI GGNCC 1 cut(s) 1093
PstI CTGCAG 1 cut(s) 362
PsuI RGATCY 1 cut(s) 1200
RsaI GTAC 6 cut(s) 188, 941, 967, 1040, 1142, 1247
RsaNI GTAC 6 cut(s) 187, 940, 966, 1039, 1141, 1246
RseI CAYNNNNRTG 1 cut(s) 947
SacI GAGCTC 1 cut(s) 287
SaqAI TTAA 5 cut(s) 26, 381, 456, 906, 1419
SatI GCNGC 4 cut(s) 239, 366, 528, 726
Sau3AI GATC 5 cut(s) 141, 208, 901, 1200, 1305
Sau96I GGNCC 1 cut(s) 1093
ScrFI CCNGG 3 cut(s) 717, 1198, 1303
SduI GDGCHC 5 cut(s) 55, 255, 287, 506, 820
SfcI CTRYAG 1 cut(s) 358
SmiMI CAYNNNNRTG 1 cut(s) 947
SmlI CTYRAG 2 cut(s) 380, 905
SmoI CTYRAG 2 cut(s) 380, 905
Sse9I AATT 5 cut(s) 850, 1056, 1080, 1183, 1288
SseBI AGGCCT 2 cut(s) 356, 1196
SsiI CCGC 3 cut(s) 37, 326, 527
SspI AATATT 1 cut(s) 30
SspMI CTAG 1 cut(s) 990
SstI GAGCTC 1 cut(s) 287
StuI AGGCCT 2 cut(s) 356, 1196
StyD4I CCNGG 3 cut(s) 715, 1196, 1301
TaaI ACNGT 3 cut(s) 91, 625, 815
TaqI TCGA 3 cut(s) 289, 509, 640
TasI AATT 5 cut(s) 850, 1056, 1080, 1183, 1288
TatI WGTACW 4 cut(s) 939, 1038, 1140, 1245
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 3 cut(s) 952, 1159, 1264
Tru1I TTAA 5 cut(s) 26, 381, 456, 906, 1419
Tru9I TTAA 5 cut(s) 26, 381, 456, 906, 1419
TscAI CASTG 2 cut(s) 253, 367
TseFI GTSAC 1 cut(s) 107
TseI GCWGC 3 cut(s) 238, 365, 725
Tsp45I GTSAC 1 cut(s) 107
TspDTI ATGAA 7 cut(s) 402, 824, 944, 1016, 1049, 1151, 1256
TspGWI ACGGA 1 cut(s) 212
TspRI CASTG 2 cut(s) 253, 367
Vha464I CTTAAG 2 cut(s) 380, 905
XapI RAATTY 3 cut(s) 1080, 1183, 1288
XspI CTAG 1 cut(s) 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.