Rorug04G0238200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009383
Physical Location & Seq
Reverse (-)
40469570 .. 40472264
2695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0238200.1

Sequence Viewer

Length: 735 bp
ATGAATAGCGAAACCTACGACAGAAGTGAATTTTGTTTCACCATTGTAGCTACAACTGCTTGTGATATGGGTACTCAGACTTTGCTCTTCACGGGAGATTATATAGTAATTACTACAAGTACGTCGGCAGGCTTAGATCAGATTTTACCATGGATGTTTTATCACAAGGTTATTGGAGTTTCCACCTTTTTTCTTTTTGTGGAAGGAAAGGCTACGTCTCCTGAAGTATCCAAAGTTCTGGAGTCTATACCTGGAGTAAAATTGATATACAGAACAAAGGAGCTCGAGGAGCAACAAGCTAGAAGTCAGATTTGGAATGAGACATGGCTGTCAAGTTTCTTTTACAAACCATGCAATTATGAGCTATTTGTGAAGCAATCTCTCAATATGGAGATGGCTATTGTTATGGCAAGGGATGCTGGAATGGACTGGATTATTCATCTTGATACTGATGAGTTACTACACCCAGCTGGTGCCAAGGAGTATTCTTTGAGGCAGTTGTTGCTTGATGTGCCTGGGAATGTGGATATGGTTATTTTTTCGAACTATGAGAGCAGTGTTGAACGAGATGATATTAAGGAACCTTTTACCGAGGTATCCATGTTCAAGAAGAATTACGATCATGTACCGAAAGATACATACTTTGGAATGTACAAAGAATCAACTCGTGGCAACCCAAACTACTTTCTGACTTATGGAAATGGGAAATCAGCTATTTCATTTTTGTATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

27.98

Weight (kDa)

4.84

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 328
AccB1I GGYRCC 1 cut(s) 473
AcsI RAATTY 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 4 cut(s) 73, 121, 627, 653
AgsI TTSAA 2 cut(s) 563, 607
AjnI CCWGG 2 cut(s) 250, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 295, 327
AluBI AGCT 6 cut(s) 50, 283, 299, 364, 470, 713
AluI AGCT 6 cut(s) 50, 283, 299, 364, 470, 713
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 2 cut(s) 222, 314
Ama87I CYCGRG 1 cut(s) 284
ApoI RAATTY 1 cut(s) 29
AsuHPI GGTGA 1 cut(s) 31
AsuII TTCGAA 1 cut(s) 542
AvaI CYCGRG 1 cut(s) 284
BaeI ACNNNNGTAYC 2 cut(s) 579, 612
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 1 cut(s) 285
BauI CACGAG 1 cut(s) 666
Bbv12I GWGCWC 1 cut(s) 285
BccI CCATC 1 cut(s) 388
BciT130I CCWGG 2 cut(s) 252, 516
BciVI GTATCC 2 cut(s) 238, 607
BcoDI GTCTC 2 cut(s) 222, 314
BfaI CTAG 1 cut(s) 300
BfuI GTATCC 2 cut(s) 238, 607
Bme1390I CCNGG 2 cut(s) 252, 516
BmeT110I CYCGRG 1 cut(s) 284
BmiI GGNNCC 2 cut(s) 475, 582
BmrFI CCNGG 2 cut(s) 252, 516
BmsI GCATC 1 cut(s) 406
BpmI CTGGAG 2 cut(s) 260, 273
Bpu14I TTCGAA 1 cut(s) 542
BsaJI CCNNGG 4 cut(s) 149, 477, 515, 591
Bse1I ACTGG 1 cut(s) 434
BseBI CCWGG 2 cut(s) 252, 516
BseDI CCNNGG 4 cut(s) 149, 477, 515, 591
BseGI GGATG 2 cut(s) 159, 421
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 1 cut(s) 434
BseRI GAGGAG 1 cut(s) 302
BseYI CCCAGC 1 cut(s) 466
BshNI GGYRCC 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 284
BsmAI GTCTC 2 cut(s) 222, 314
BsmBI CGTCTC 1 cut(s) 222
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 285
Bsp1407I TGTACA 1 cut(s) 651
Bsp143I GATC 2 cut(s) 136, 619
Bsp19I CCATGG 1 cut(s) 149
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 2 cut(s) 475, 582
BspQI GCTCTTC 1 cut(s) 92
BspT104I TTCGAA 1 cut(s) 542
BspT107I GGYRCC 1 cut(s) 473
BsrGI TGTACA 1 cut(s) 651
BsrI ACTGG 1 cut(s) 434
BssECI CCNNGG 4 cut(s) 149, 477, 515, 591
BssMI GATC 2 cut(s) 136, 619
BssSI CACGAG 1 cut(s) 666
BssT1I CCWWGG 2 cut(s) 149, 477
Bst2BI CACGAG 1 cut(s) 666
Bst2UI CCWGG 2 cut(s) 252, 516
Bst6I CTCTTC 1 cut(s) 92
BstAPI GCANNNNNTGC 2 cut(s) 416, 502
BstAUI TGTACA 1 cut(s) 651
BstBI TTCGAA 1 cut(s) 542
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 2 cut(s) 75, 133
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 2 cut(s) 159, 421
BstKTI GATC 2 cut(s) 139, 622
BstMAI GTCTC 2 cut(s) 222, 314
BstMBI GATC 2 cut(s) 136, 619
BstMWI GCNNNNNNNGC 5 cut(s) 56, 289, 416, 502, 511
BstNI CCWGG 2 cut(s) 252, 516
BstSCI CCNGG 2 cut(s) 250, 514
BstXI CCANNNNNNTGG 1 cut(s) 238
BsuI GTATCC 2 cut(s) 238, 607
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 2 cut(s) 159, 421
BtsI GCAGTG 1 cut(s) 562
BtsIMutI CAGTG 1 cut(s) 562
Cac8I GCNNGC 1 cut(s) 130
Csp6I GTAC 4 cut(s) 72, 120, 626, 652
CviAII CATG 5 cut(s) 150, 324, 351, 601, 623
CviQI GTAC 4 cut(s) 72, 120, 626, 652
DdeI CTNAG 2 cut(s) 75, 133
DpnI GATC 2 cut(s) 138, 621
DpnII GATC 2 cut(s) 136, 619
DrdI GACNNNNNNGTC 1 cut(s) 328
DseDI GACNNNNNNGTC 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 149, 477
Eco24I GRGCYC 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 284
EcoICRI GAGCTC 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 250, 514
EcoT14I CCWWGG 2 cut(s) 149, 477
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 2 cut(s) 149, 477
Esp3I CGTCTC 1 cut(s) 222
FaeI CATG 5 cut(s) 153, 327, 354, 604, 626
FatI CATG 5 cut(s) 149, 323, 350, 600, 622
FokI GGATG 2 cut(s) 166, 428
FriOI GRGCYC 1 cut(s) 285
FspBI CTAG 1 cut(s) 300
GsaI CCCAGC 1 cut(s) 470
GsuI CTGGAG 2 cut(s) 260, 273
Hin1II CATG 5 cut(s) 153, 327, 354, 604, 626
HinfI GANTC 2 cut(s) 242, 659
HphI GGTGA 1 cut(s) 31
Hpy188I TCNGA 4 cut(s) 78, 141, 309, 690
Hpy188III TCNNGA 4 cut(s) 221, 239, 443, 607
Hpy99I CGWCG 1 cut(s) 127
HpyAV CCTTC 1 cut(s) 197
HpyCH4IV ACGT 2 cut(s) 122, 215
HpyCH4V TGCA 1 cut(s) 354
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 289, 416, 502, 511
HpyF3I CTNAG 2 cut(s) 75, 133
HpySE526I ACGT 2 cut(s) 122, 215
Hsp92II CATG 5 cut(s) 153, 327, 354, 604, 626
Kzo9I GATC 2 cut(s) 136, 619
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 2 cut(s) 280, 289
LweI GCATC 1 cut(s) 406
MaeI CTAG 1 cut(s) 300
MaeII ACGT 2 cut(s) 122, 215
MaeIII GTNAC 1 cut(s) 456
MalI GATC 2 cut(s) 138, 621
MboI GATC 2 cut(s) 136, 619
MboII GAAGA 2 cut(s) 79, 622
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 5 cut(s) 29, 108, 260, 355, 613
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 3 cut(s) 280, 486, 586
MseI TTAA 1 cut(s) 576
MspA1I CMGCKG 1 cut(s) 470
MspR9I CCNGG 2 cut(s) 252, 516
MvaI CCWGG 2 cut(s) 252, 516
MwoI GCNNNNNNNGC 5 cut(s) 56, 289, 416, 502, 511
NcoI CCATGG 1 cut(s) 149
NdeII GATC 2 cut(s) 136, 619
NlaIII CATG 5 cut(s) 153, 327, 354, 604, 626
NlaIV GGNNCC 2 cut(s) 475, 582
NspV TTCGAA 1 cut(s) 542
PaeR7I CTCGAG 1 cut(s) 284
PciSI GCTCTTC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 659
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 2 cut(s) 250, 514
PspFI CCCAGC 1 cut(s) 466
PspGI CCWGG 2 cut(s) 250, 514
PspN4I GGNNCC 2 cut(s) 475, 582
PspXI VCTCGAGB 1 cut(s) 284
PsrI GAACNNNNNNTAC 2 cut(s) 219, 251
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 4 cut(s) 73, 121, 627, 653
RsaNI GTAC 4 cut(s) 72, 120, 626, 652
SacI GAGCTC 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 1 cut(s) 576
Sau3AI GATC 2 cut(s) 136, 619
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 2 cut(s) 252, 516
SduI GDGCHC 1 cut(s) 285
SfaNI GCATC 1 cut(s) 406
Sfr274I CTCGAG 1 cut(s) 284
SfuI TTCGAA 1 cut(s) 542
SlaI CTCGAG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 5 cut(s) 29, 108, 260, 355, 613
SspMI CTAG 1 cut(s) 300
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 250, 514
StyI CCWWGG 2 cut(s) 149, 477
TaiI ACGT 2 cut(s) 125, 218
TaqI TCGA 2 cut(s) 285, 542
TasI AATT 5 cut(s) 29, 108, 260, 355, 613
TatI WGTACW 1 cut(s) 651
TfiI GAWTC 1 cut(s) 659
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TscAI CASTG 1 cut(s) 562
TspDTI ATGAA 3 cut(s) 17, 428, 708
TspRI CASTG 1 cut(s) 562
XapI RAATTY 1 cut(s) 29
XhoI CTCGAG 1 cut(s) 284
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.