FvH4_4g23410

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009383
Physical Location & Seq
Forward (+)
25898165 .. 25900391
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23410.t3

Sequence Viewer

Length: 843 bp
ATGTCTGGCGTATCGGTCGCTGTGGCTCCTAGGTCGGAGCCTGATAAAACCACAACTGCTGGACCAAAACACCAAGAAAAGCCGCAACGCCTACTGCACCAACAGCAGCAGCAGCCGGCTGTAGGAGGTGTCATGGGGTCATTGCGCGTAATCGAGCTCCAGCTTGTGGCATTCATCATGGTGTTCTCAGCCAGTGGCCTTGTCCCGCTCCTTGATTTGATATTCCCAGCTTTCGCCTCTGCCTATCTCTTAGTTTTGTCACGCTTGGCCTTCCCATCACATGGTCACGTCTCTTCTGGCTCGCAGGAGATTTTCCAAGGAAGCAAATTCTTCAGGCTGTATGTGATTGCGGGAACTACGGTTGGACTGTTCTTGCCTCTGGCTTATGTGTTGGGTGGTTTTGCAAGGGGTGATGAGCACGCTGTGCGCTCAGCAACACCTCACTTGTTCTTGCTCTCCTTTCAGATTCTGACTGAAAATGTCATAAGCGGTTTGTCACTATTTTCGCCACCAGTTAGAGCGCTTGTTCCGTTGCTATACACAGTTAGGAGAATCTTCGTCATCCTTGATTGGGTGAATGATGTCTGGCTTAACAAGACTCTTCCTGCAAATGCACATGTCAAGGATGTTGCATGGTTTTGGTTTGGGAGGACGTTGGCTGTGGCCAACTTCTCATATTTCGCTCTCAATCTGTTCGGGTTTTTGATTCCTCGGTTTCTTCCAAGGGCCTTTGAGAAGTACATCCGGGAGGGGGATGAAGTTCATTCCAAGACTGCAGAGGACAAGCGCTCAGCAGCACTAAATAAATCGGAATCAGCTCCGGCAGATAAGAAGGCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

30.88

Weight (kDa)

9.58

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 49 - 248 5.9e-80 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 166, 281
AccBSI CCGCTC 1 cut(s) 208
AccII CGCG 1 cut(s) 147
AciI CCGC 4 cut(s) 83, 206, 350, 489
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 316
AdeI CACNNNGTG 1 cut(s) 424
AfaI GTAC 1 cut(s) 740
AfeI AGCGCT 2 cut(s) 522, 788
AfiI CCNNNNNNNGG 5 cut(s) 122, 166, 281, 571, 751
AflIII ACRYGT 1 cut(s) 616
AjiI CACGTC 1 cut(s) 289
AluBI AGCT 4 cut(s) 157, 163, 230, 818
AluI AGCT 4 cut(s) 157, 163, 230, 818
Alw21I GWGCWC 2 cut(s) 159, 420
Alw26I GTCTC 1 cut(s) 295
AlwNI CAGNNNCTG 1 cut(s) 469
Aor51HI AGCGCT 2 cut(s) 522, 788
AoxI GGCC 4 cut(s) 196, 267, 663, 726
ApeKI GCWGC 4 cut(s) 106, 109, 112, 794
ApoI RAATTY 1 cut(s) 326
AspA2I CCTAGG 1 cut(s) 29
AspLEI GCGC 4 cut(s) 147, 429, 523, 789
AspS9I GGNCC 2 cut(s) 62, 726
AsuC2I CCSGG 1 cut(s) 746
AsuHPI GGTGA 2 cut(s) 422, 586
AvaII GGWCC 1 cut(s) 62
AvrII CCTAGG 1 cut(s) 29
BalI TGGCCA 1 cut(s) 665
BanII GRGCYC 1 cut(s) 159
Bbv12I GWGCWC 2 cut(s) 159, 420
BbvI GCAGC 4 cut(s) 118, 121, 124, 806
BccI CCATC 1 cut(s) 283
BcnI CCSGG 1 cut(s) 746
BcoDI GTCTC 1 cut(s) 295
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 2 cut(s) 120, 774
BfoI RGCGCY 2 cut(s) 524, 790
BisI GCNGC 5 cut(s) 83, 107, 110, 113, 795
BlnI CCTAGG 1 cut(s) 29
BlpI GCTNAGC 2 cut(s) 430, 790
BlsI GCNGC 5 cut(s) 84, 108, 111, 114, 796
Bme1390I CCNGG 1 cut(s) 746
Bme18I GGWCC 1 cut(s) 62
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 2 cut(s) 62, 726
BmiI GGNNCC 2 cut(s) 27, 39
BmrFI CCNGG 1 cut(s) 746
BpmI CTGGAG 1 cut(s) 143
Bpu1102I GCTNAGC 2 cut(s) 430, 790
BpuMI CCSGG 1 cut(s) 746
BsaJI CCNNGG 4 cut(s) 29, 316, 710, 722
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 166, 281, 571, 751
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 2 cut(s) 192, 512
Bse3DI GCAATG 1 cut(s) 140
BseDI CCNNGG 4 cut(s) 29, 316, 710, 722
BseGI GGATG 4 cut(s) 561, 631, 741, 760
BseLI CCNNNNNNNGG 5 cut(s) 122, 166, 281, 571, 751
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 3 cut(s) 201, 444, 804
BseNI ACTGG 2 cut(s) 192, 512
BseXI GCAGC 4 cut(s) 118, 121, 124, 806
BseYI CCCAGC 1 cut(s) 226
BsgI GTGCAG 1 cut(s) 80
Bsh1236I CGCG 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 4 cut(s) 198, 269, 665, 728
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 2 cut(s) 159, 420
BsiSI CCGG 3 cut(s) 116, 745, 821
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 5 cut(s) 122, 166, 281, 571, 751
BsmAI GTCTC 1 cut(s) 295
BsmBI CGTCTC 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 188
BsmI GAATGC 1 cut(s) 170
BsnI GGCC 4 cut(s) 198, 269, 665, 728
Bsp1286I GDGCHC 2 cut(s) 159, 420
Bsp1720I GCTNAGC 2 cut(s) 430, 790
BspACI CCGC 4 cut(s) 83, 206, 350, 489
BspANI GGCC 4 cut(s) 198, 269, 665, 728
BspCNI CTCAG 3 cut(s) 200, 443, 803
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 2 cut(s) 27, 39
BspMAI CTGCAG 1 cut(s) 778
BsrBI CCGCTC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 2 cut(s) 192, 512
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 4 cut(s) 29, 316, 710, 722
BssT1I CCWWGG 3 cut(s) 29, 316, 722
Bst4CI ACNGT 3 cut(s) 361, 369, 544
Bst6I CTCTTC 2 cut(s) 298, 606
BstAPI GCANNNNNTGC 1 cut(s) 424
BstC8I GCNNGC 3 cut(s) 117, 302, 420
BstDEI CTNAG 4 cut(s) 187, 250, 430, 790
BstF5I GGATG 4 cut(s) 561, 631, 741, 760
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 2 cut(s) 524, 790
BstHHI GCGC 4 cut(s) 147, 429, 523, 789
BstMAI GTCTC 1 cut(s) 295
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 3 cut(s) 103, 112, 424
BstNSI RCATGY 1 cut(s) 620
BstSCI CCNGG 1 cut(s) 744
BstSFI CTRYAG 2 cut(s) 120, 774
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 4 cut(s) 118, 121, 124, 806
BsuRI GGCC 4 cut(s) 198, 269, 665, 728
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 4 cut(s) 561, 631, 741, 760
BtsIMutI CAGTG 1 cut(s) 199
Cac8I GCNNGC 3 cut(s) 117, 302, 420
CaiI CAGNNNCTG 1 cut(s) 469
CfoI GCGC 4 cut(s) 147, 429, 523, 789
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 2 cut(s) 62, 726
Csp6I GTAC 1 cut(s) 739
CviAII CATG 5 cut(s) 133, 178, 281, 617, 633
CviQI GTAC 1 cut(s) 739
DdeI CTNAG 4 cut(s) 187, 250, 430, 790
DraIII CACNNNGTG 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 663
Eam1104I CTCTTC 2 cut(s) 298, 606
EarI CTCTTC 2 cut(s) 298, 606
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 3 cut(s) 29, 316, 722
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 62
Eco47III AGCGCT 2 cut(s) 522, 788
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 316
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 726
EcoT14I CCWWGG 3 cut(s) 29, 316, 722
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 3 cut(s) 29, 316, 722
Esp3I CGTCTC 1 cut(s) 295
FaeI CATG 5 cut(s) 136, 181, 284, 620, 636
FaqI GGGAC 1 cut(s) 188
FatI CATG 5 cut(s) 132, 177, 280, 616, 632
FauI CCCGC 2 cut(s) 213, 343
Fnu4HI GCNGC 5 cut(s) 83, 107, 110, 113, 795
FokI GGATG 4 cut(s) 548, 638, 728, 767
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 5 cut(s) 83, 107, 110, 113, 795
FspBI CTAG 1 cut(s) 30
GlaI GCGC 4 cut(s) 146, 428, 522, 788
GluI GCNGC 5 cut(s) 83, 107, 110, 113, 795
GsaI CCCAGC 1 cut(s) 230
GsuI CTGGAG 1 cut(s) 143
HaeII RGCGCY 2 cut(s) 524, 790
HaeIII GGCC 4 cut(s) 198, 269, 665, 728
HapII CCGG 3 cut(s) 116, 745, 821
HhaI GCGC 4 cut(s) 147, 429, 523, 789
Hin1II CATG 5 cut(s) 136, 181, 284, 620, 636
Hin6I GCGC 4 cut(s) 145, 427, 521, 787
HinP1I GCGC 4 cut(s) 145, 427, 521, 787
HinfI GANTC 5 cut(s) 466, 552, 598, 706, 812
HpaII CCGG 3 cut(s) 116, 745, 821
HphI GGTGA 2 cut(s) 422, 586
Hpy188I TCNGA 4 cut(s) 37, 465, 471, 811
HpyAV CCTTC 2 cut(s) 280, 826
HpyCH4III ACNGT 3 cut(s) 361, 369, 544
HpyCH4IV ACGT 2 cut(s) 288, 653
HpyCH4V TGCA 6 cut(s) 97, 404, 608, 614, 632, 776
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 112, 424
HpyF3I CTNAG 4 cut(s) 187, 250, 430, 790
HpySE526I ACGT 2 cut(s) 288, 653
Hsp92II CATG 5 cut(s) 136, 181, 284, 620, 636
HspAI GCGC 4 cut(s) 145, 427, 521, 787
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
LmnI GCTCC 5 cut(s) 31, 37, 162, 213, 823
Lsp1109I GCAGC 4 cut(s) 118, 121, 124, 806
MaeI CTAG 1 cut(s) 30
MaeII ACGT 2 cut(s) 288, 653
MaeIII GTNAC 3 cut(s) 258, 284, 495
MbiI CCGCTC 1 cut(s) 208
MboII GAAGA 5 cut(s) 285, 322, 547, 593, 710
MhlI GDGCHC 2 cut(s) 159, 420
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 1 cut(s) 326
MluNI TGGCCA 1 cut(s) 665
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 2 cut(s) 15, 343
MnlI CCTC 8 cut(s) 119, 247, 387, 450, 642, 720, 742, 772
Mox20I TGGCCA 1 cut(s) 665
MroNI GCCGGC 1 cut(s) 115
MscI TGGCCA 1 cut(s) 665
MseI TTAA 1 cut(s) 591
MslI CAYNNNNRTG 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 665
MspI CCGG 3 cut(s) 116, 745, 821
MspR9I CCNGG 1 cut(s) 746
Mva1269I GAATGC 1 cut(s) 170
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 3 cut(s) 103, 112, 424
NaeI GCCGGC 1 cut(s) 117
NciI CCSGG 1 cut(s) 746
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 5 cut(s) 136, 181, 284, 620, 636
NlaIV GGNNCC 2 cut(s) 27, 39
NmuCI GTSAC 3 cut(s) 258, 284, 495
NspI RCATGY 1 cut(s) 620
PciI ACATGT 1 cut(s) 616
PctI GAATGC 1 cut(s) 170
PdiI GCCGGC 1 cut(s) 117
PfeI GAWTC 4 cut(s) 466, 552, 706, 812
PflMI CCANNNNNTGG 2 cut(s) 166, 281
PfoI TCCNGGA 1 cut(s) 744
PkrI GCNGC 5 cut(s) 84, 108, 111, 114, 796
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PscI ACATGT 1 cut(s) 616
Psp124BI GAGCTC 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 226
PspN4I GGNNCC 2 cut(s) 27, 39
PspPI GGNCC 2 cut(s) 62, 726
PstI CTGCAG 1 cut(s) 778
PstNI CAGNNNCTG 1 cut(s) 469
RsaI GTAC 1 cut(s) 740
RsaNI GTAC 1 cut(s) 739
RseI CAYNNNNRTG 1 cut(s) 179
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 5 cut(s) 83, 107, 110, 113, 795
Sau96I GGNCC 2 cut(s) 62, 726
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 1 cut(s) 746
SduI GDGCHC 2 cut(s) 159, 420
SetI ASST 9 cut(s) 35, 130, 159, 165, 232, 291, 442, 656, 820
SfcI CTRYAG 2 cut(s) 120, 774
SinI GGWCC 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 1 cut(s) 326
SsiI CCGC 4 cut(s) 83, 206, 350, 489
SspMI CTAG 1 cut(s) 30
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 744
StyI CCWWGG 3 cut(s) 29, 316, 722
TaaI ACNGT 3 cut(s) 361, 369, 544
TaiI ACGT 2 cut(s) 291, 656
TaqI TCGA 1 cut(s) 153
TaqII GACCGA 1 cut(s) 4
TasI AATT 1 cut(s) 326
TatI WGTACW 1 cut(s) 738
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 4 cut(s) 466, 552, 706, 812
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 3 cut(s) 258, 284, 495
TseI GCWGC 4 cut(s) 106, 109, 112, 794
Tsp45I GTSAC 3 cut(s) 258, 284, 495
TspDTI ATGAA 3 cut(s) 163, 752, 771
TspGWI ACGGA 1 cut(s) 519
TspRI CASTG 1 cut(s) 199
Van91I CCANNNNNTGG 2 cut(s) 166, 281
VpaK11BI GGWCC 1 cut(s) 62
XapI RAATTY 1 cut(s) 326
XceI RCATGY 1 cut(s) 620
XmaJI CCTAGG 1 cut(s) 29
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.