MD16G1123100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009383
Physical Location & Seq
Reverse (-)
8888902 .. 8890748
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1123100.v1.1.491

Sequence Viewer

Length: 843 bp
ATGTCGGGTGTGTCTCTTGCTGTGGCTCCTAGATCAGAGCCCGACACAACCACTACAACTACAACCCCTGGATCCAAACCCCAAGAGAAACCGCTCCACCGCCAACAGCACCAGCAGCCTGCTGTTGGCGGTGTAATGGGCTCATTGCGAGTAATAGAGCTCCAACTCGTGGCCTTCATCATGGTTTTCTCAGTCAGCGGCCTTGTCCCGCTTTTTGATTTGATCTTCCCGGCCTTTGCCTCCGCATATCTCTTAGCTCTGGCGCGCTTGGCCTTCCCTTCTCACGGTCCCGTCAGCTCTAGCTCTCGGGAGATTTTCCAAGGCAGCAGATTCTTCAGGTTTTACGTGGTTGTGGGAACCACAATCGGGCTCTTCTTGCCGCTTGCATACGTATTGGGTGGTTTTGCGCGGGGCGATGAGCACGCTGTCCGCTCGGCAACACCTCACTTGTTCTTGCTCTCATTTCAGATTCTCACTGAGAATGTGATTAGCGGATTGTCGCTGTTTTCTCCGCCGGTGAGGGCATTGGTCCCGTTGCTTTACACTGTCAGGAGAATCTGTGTCATCCTTGATTGGATGAAAGATGTTTGGCTTAACAAAACTCTGCCTGCCAATGCACAGTTTAAGGATGTTGCATGGTTTTGGTTTGGGAGGAGCCTGGCTATAGCCAATTTCATATATTTCTCGATCAACCTATTCGGGTTTTTGATCCCTCGATTCCTTCCGAGGGCATTTGAGAGATACTTCAGAGAGAGGGATGAAGTTGAGTCCAAGATGGTAGAGGACAAGCGCTCCGCAGCAGCAAGTAAGTTGGAACCAGTTGCAGATAAGAAAAGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

31.27

Weight (kDa)

9.78

Isoelectric Point (pI)

49.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 50 - 249 1.2e-79 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 169
AccBSI CCGCTC 2 cut(s) 94, 432
AccII CGCG 2 cut(s) 265, 409
AclWI GGATC 3 cut(s) 66, 79, 703
AcuI CTGAAG 2 cut(s) 319, 730
AfeI AGCGCT 1 cut(s) 791
AfiI CCNNNNNNNGG 5 cut(s) 125, 169, 284, 366, 727
AjnI CCWGG 2 cut(s) 67, 657
AluBI AGCT 4 cut(s) 160, 257, 297, 303
AluI AGCT 4 cut(s) 160, 257, 297, 303
Alw21I GWGCWC 2 cut(s) 162, 423
Alw26I GTCTC 1 cut(s) 18
AlwI GGATC 3 cut(s) 66, 79, 703
Ama87I CYCGRG 1 cut(s) 306
Aor51HI AGCGCT 1 cut(s) 791
AoxI GGCC 4 cut(s) 171, 199, 231, 270
ApeKI GCWGC 4 cut(s) 115, 324, 797, 800
AspLEI GCGC 4 cut(s) 265, 267, 409, 792
AspS9I GGNCC 2 cut(s) 287, 529
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 1 cut(s) 529
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 2 cut(s) 287, 529
BamHI GGATCC 1 cut(s) 71
BanII GRGCYC 4 cut(s) 42, 143, 162, 372
BauI CACGAG 1 cut(s) 167
Bbv12I GWGCWC 2 cut(s) 162, 423
BbvI GCAGC 4 cut(s) 127, 336, 809, 812
BccI CCATC 1 cut(s) 769
BciT130I CCWGG 2 cut(s) 69, 659
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 1 cut(s) 18
BfaI CTAG 2 cut(s) 30, 300
BfmI CTRYAG 1 cut(s) 663
BfoI RGCGCY 1 cut(s) 793
BisI GCNGC 6 cut(s) 116, 199, 325, 380, 798, 801
BlsI GCNGC 6 cut(s) 117, 200, 326, 381, 799, 802
Bme1390I CCNGG 3 cut(s) 69, 230, 659
Bme18I GGWCC 2 cut(s) 287, 529
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 2 cut(s) 287, 529
BmiI GGNNCC 7 cut(s) 27, 73, 289, 358, 531, 656, 816
BmrFI CCNGG 3 cut(s) 69, 230, 659
BpuMI CCSGG 1 cut(s) 230
BsaAI YACGTR 2 cut(s) 346, 391
BsaJI CCNNGG 3 cut(s) 67, 319, 725
BsaXI ACNNNNNCTCC 4 cut(s) 544, 574, 776, 806
Bsc4I CCNNNNNNNGG 5 cut(s) 125, 169, 284, 366, 727
Bse118I RCCGGY 1 cut(s) 514
Bse1I ACTGG 1 cut(s) 818
Bse3DI GCAATG 1 cut(s) 143
BseBI CCWGG 2 cut(s) 69, 659
BseDI CCNNGG 3 cut(s) 67, 319, 725
BseGI GGATG 4 cut(s) 564, 582, 634, 763
BseLI CCNNNNNNNGG 5 cut(s) 125, 169, 284, 366, 727
BseMI GCAATG 1 cut(s) 143
BseMII CTCAG 2 cut(s) 204, 468
BseNI ACTGG 1 cut(s) 818
BsePI GCGCGC 1 cut(s) 263
BseRI GAGGAG 1 cut(s) 667
BseXI GCAGC 4 cut(s) 127, 336, 809, 812
Bsh1236I CGCG 2 cut(s) 265, 409
BshFI GGCC 4 cut(s) 173, 201, 233, 272
BsiHKAI GWGCWC 2 cut(s) 162, 423
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 2 cut(s) 230, 515
BslFI GGGAC 3 cut(s) 191, 273, 515
BslI CCNNNNNNNGG 5 cut(s) 125, 169, 284, 366, 727
BsmAI GTCTC 1 cut(s) 18
BsmFI GGGAC 3 cut(s) 191, 273, 515
BsnI GGCC 4 cut(s) 173, 201, 233, 272
BsoBI CYCGRG 1 cut(s) 306
Bsp1286I GDGCHC 5 cut(s) 42, 143, 162, 372, 423
Bsp143I GATC 5 cut(s) 32, 71, 222, 687, 708
BspANI GGCC 4 cut(s) 173, 201, 233, 272
BspCNI CTCAG 2 cut(s) 203, 469
BspFNI CGCG 2 cut(s) 265, 409
BspLI GGNNCC 7 cut(s) 27, 73, 289, 358, 531, 656, 816
BspPI GGATC 3 cut(s) 66, 79, 703
BspQI GCTCTTC 1 cut(s) 377
BsrBI CCGCTC 2 cut(s) 94, 432
BsrDI GCAATG 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 514
BsrI ACTGG 1 cut(s) 818
BssAI RCCGGY 1 cut(s) 514
BssECI CCNNGG 3 cut(s) 67, 319, 725
BssHII GCGCGC 1 cut(s) 263
BssMI GATC 5 cut(s) 32, 71, 222, 687, 708
BssSI CACGAG 1 cut(s) 167
BssT1I CCWWGG 1 cut(s) 319
Bst2BI CACGAG 1 cut(s) 167
Bst2UI CCWGG 2 cut(s) 69, 659
Bst4CI ACNGT 3 cut(s) 287, 547, 621
Bst6I CTCTTC 1 cut(s) 377
BstBAI YACGTR 2 cut(s) 346, 391
BstC8I GCNNGC 5 cut(s) 120, 265, 384, 423, 609
BstDEI CTNAG 3 cut(s) 190, 253, 477
BstF5I GGATG 4 cut(s) 564, 582, 634, 763
BstFNI CGCG 2 cut(s) 265, 409
BstH2I RGCGCY 1 cut(s) 793
BstHHI GCGC 4 cut(s) 265, 267, 409, 792
BstKTI GATC 5 cut(s) 35, 74, 225, 690, 711
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 5 cut(s) 32, 71, 222, 687, 708
BstMWI GCNNNNNNNGC 3 cut(s) 115, 269, 376
BstNI CCWGG 2 cut(s) 69, 659
BstSCI CCNGG 3 cut(s) 67, 228, 657
BstSFI CTRYAG 1 cut(s) 663
BstSNI TACGTA 1 cut(s) 391
BstUI CGCG 2 cut(s) 265, 409
BstV1I GCAGC 4 cut(s) 127, 336, 809, 812
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
BsuRI GGCC 4 cut(s) 173, 201, 233, 272
BtgZI GCGATG 1 cut(s) 429
BtsCI GGATG 4 cut(s) 564, 582, 634, 763
BtsIMutI CAGTG 2 cut(s) 474, 543
Cac8I GCNNGC 5 cut(s) 120, 265, 384, 423, 609
CfoI GCGC 4 cut(s) 265, 267, 409, 792
Cfr10I RCCGGY 1 cut(s) 514
Cfr13I GGNCC 2 cut(s) 287, 529
CviAII CATG 2 cut(s) 181, 636
DdeI CTNAG 3 cut(s) 190, 253, 477
DpnI GATC 5 cut(s) 34, 73, 224, 689, 710
DpnII GATC 5 cut(s) 32, 71, 222, 687, 708
Eam1104I CTCTTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 377
EciI GGCGGA 1 cut(s) 501
Ecl136II GAGCTC 1 cut(s) 160
Eco105I TACGTA 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 319
Eco24I GRGCYC 4 cut(s) 42, 143, 162, 372
Eco47I GGWCC 2 cut(s) 287, 529
Eco47III AGCGCT 1 cut(s) 791
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 319, 730
Eco88I CYCGRG 1 cut(s) 306
EcoICRI GAGCTC 1 cut(s) 160
EcoRII CCWGG 2 cut(s) 67, 657
EcoT14I CCWWGG 1 cut(s) 319
EcoT38I GRGCYC 4 cut(s) 42, 143, 162, 372
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 2 cut(s) 184, 639
FaiI YATR 7 cut(s) 182, 247, 388, 637, 665, 677, 679
FaqI GGGAC 3 cut(s) 191, 273, 515
FatI CATG 2 cut(s) 180, 635
FauI CCCGC 2 cut(s) 216, 402
Fnu4HI GCNGC 6 cut(s) 116, 199, 325, 380, 798, 801
FokI GGATG 4 cut(s) 551, 589, 641, 770
FriOI GRGCYC 4 cut(s) 42, 143, 162, 372
Fsp4HI GCNGC 6 cut(s) 116, 199, 325, 380, 798, 801
FspBI CTAG 2 cut(s) 30, 300
GlaI GCGC 4 cut(s) 264, 266, 408, 791
GluI GCNGC 6 cut(s) 116, 199, 325, 380, 798, 801
HaeII RGCGCY 1 cut(s) 793
HaeIII GGCC 4 cut(s) 173, 201, 233, 272
HapII CCGG 2 cut(s) 230, 515
HhaI GCGC 4 cut(s) 265, 267, 409, 792
Hin1II CATG 2 cut(s) 184, 639
Hin6I GCGC 4 cut(s) 263, 265, 407, 790
HinP1I GCGC 4 cut(s) 263, 265, 407, 790
HinfI GANTC 5 cut(s) 330, 469, 555, 717, 767
HpaII CCGG 2 cut(s) 230, 515
HphI GGTGA 1 cut(s) 529
Hpy188I TCNGA 4 cut(s) 37, 468, 726, 749
Hpy188III TCNNGA 3 cut(s) 308, 550, 685
HpyAV CCTTC 4 cut(s) 184, 283, 288, 731
HpyCH4III ACNGT 3 cut(s) 287, 547, 621
HpyCH4IV ACGT 2 cut(s) 345, 390
HpyCH4V TGCA 4 cut(s) 386, 617, 635, 824
HpyF10VI GCNNNNNNNGC 3 cut(s) 115, 269, 376
HpyF3I CTNAG 3 cut(s) 190, 253, 477
HpySE526I ACGT 2 cut(s) 345, 390
Hsp92II CATG 2 cut(s) 184, 639
HspAI GCGC 4 cut(s) 263, 265, 407, 790
Kzo9I GATC 5 cut(s) 32, 71, 222, 687, 708
LguI GCTCTTC 1 cut(s) 377
LmnI GCTCC 5 cut(s) 31, 99, 165, 654, 797
Lsp1109I GCAGC 4 cut(s) 127, 336, 809, 812
MaeI CTAG 2 cut(s) 30, 300
MaeII ACGT 2 cut(s) 345, 390
MalI GATC 5 cut(s) 34, 73, 224, 689, 710
MbiI CCGCTC 2 cut(s) 94, 432
MboI GATC 5 cut(s) 32, 71, 222, 687, 708
MboII GAAGA 3 cut(s) 217, 325, 364
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 5 cut(s) 42, 143, 162, 372, 423
MluCI AATT 1 cut(s) 670
MlyI GAGTC 1 cut(s) 776
MmeI TCCRAC 2 cut(s) 187, 792
MnlI CCTC 8 cut(s) 250, 453, 513, 645, 720, 723, 747, 775
MseI TTAA 2 cut(s) 594, 624
MspA1I CMGCKG 1 cut(s) 198
MspI CCGG 2 cut(s) 230, 515
MspR9I CCNGG 3 cut(s) 69, 230, 659
MvaI CCWGG 2 cut(s) 69, 659
MvnI CGCG 2 cut(s) 265, 409
MwoI GCNNNNNNNGC 3 cut(s) 115, 269, 376
NciI CCSGG 1 cut(s) 230
NdeII GATC 5 cut(s) 32, 71, 222, 687, 708
NlaIII CATG 2 cut(s) 184, 639
NlaIV GGNNCC 7 cut(s) 27, 73, 289, 358, 531, 656, 816
NmeAIII GCCGAG 1 cut(s) 413
PauI GCGCGC 1 cut(s) 263
PciSI GCTCTTC 1 cut(s) 377
PfeI GAWTC 4 cut(s) 330, 469, 555, 717
PflMI CCANNNNNTGG 1 cut(s) 169
PkrI GCNGC 6 cut(s) 117, 200, 326, 381, 799, 802
PleI GAGTC 1 cut(s) 775
PpsI GAGTC 1 cut(s) 775
Ppu21I YACGTR 2 cut(s) 346, 391
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 67, 657
PspGI CCWGG 2 cut(s) 67, 657
PspN4I GGNNCC 7 cut(s) 27, 73, 289, 358, 531, 656, 816
PspPI GGNCC 2 cut(s) 287, 529
PsuI RGATCY 1 cut(s) 71
PteI GCGCGC 1 cut(s) 263
SacI GAGCTC 1 cut(s) 162
SapI GCTCTTC 1 cut(s) 377
SaqAI TTAA 2 cut(s) 594, 624
SatI GCNGC 6 cut(s) 116, 199, 325, 380, 798, 801
Sau3AI GATC 5 cut(s) 32, 71, 222, 687, 708
Sau96I GGNCC 2 cut(s) 287, 529
SchI GAGTC 1 cut(s) 776
ScrFI CCNGG 3 cut(s) 69, 230, 659
SduI GDGCHC 5 cut(s) 42, 143, 162, 372, 423
SetI ASST 9 cut(s) 162, 259, 299, 305, 341, 348, 393, 445, 696
SfcI CTRYAG 1 cut(s) 663
SgrAI CRCCGGYG 1 cut(s) 514
SinI GGWCC 2 cut(s) 287, 529
SnaBI TACGTA 1 cut(s) 391
Sse9I AATT 1 cut(s) 670
SspMI CTAG 2 cut(s) 30, 300
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 67, 228, 657
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 3 cut(s) 287, 547, 621
TaiI ACGT 2 cut(s) 348, 393
TaqI TCGA 2 cut(s) 686, 715
TasI AATT 1 cut(s) 670
TauI GCSGC 2 cut(s) 201, 382
TfiI GAWTC 4 cut(s) 330, 469, 555, 717
Tru1I TTAA 2 cut(s) 594, 624
Tru9I TTAA 2 cut(s) 594, 624
TscAI CASTG 2 cut(s) 481, 550
TseI GCWGC 4 cut(s) 115, 324, 797, 800
TspDTI ATGAA 4 cut(s) 166, 593, 664, 774
TspRI CASTG 2 cut(s) 481, 550
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 2 cut(s) 287, 529
XspI CTAG 2 cut(s) 30, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.