MD13G1122600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009383
Physical Location & Seq
Reverse (-)
9046892 .. 9048728
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1122600.v1.1.491

Sequence Viewer

Length: 837 bp
ATGTCAGGTGTATCTCTTGCCGTGCCTCCTAGATCGGAGCCCGACACAACCACCACAACCCCAGGATCCAAACCCCAACAGAAACCGCTCCACCGCCAACAGCACCAGCAGTCTGCTGTAGGCGGTGTAATGGGTTCACTGCGAGTAATAGAGCTCCAACTCGTGGCCTTCATCATGGTTTTCTCGGCCAGTGGCCTTGTCCCGCTTTTTGATTTGATCTTCCCGGCTTTTGCCACCGCATACCTTTTAGGCCTGTCACGCTTGGCGTTCCCTTCCCACGGCGCTGTCAGCACTGGCTCTCAGGAGATTTTCCATGGCAGCAGATTCTTCAGGTTCTATGTGATTGTGGGAACCACAGTTGGGCTCTTCTTGCCGCTTGCATACGTGTTGGGTGGCTTTGCGAGGGGTGATGAGCACGCTGTCCGCTCTGCAACGCCTCACTTGTTCTTGCTTTCGTGTCAGATTCTGACTGAGAATGTGATTAGCGGGTTGTCGCTGTTTTCACCGCCAGTGAGGGCATTGGTCCCATTGCTTTACACTGTCAGGAGGATCTTTGTCGTCCTTGATTGGATGAAAGATGTTTGGCTTAACAAAACTCTGCCTGCCAATGCACAGTTTAAGGATGTTGCATGGTTTTGGTTTGGGAGAAGCCTGGCTGTAGCCAATTTCTTGTATTTCTCGATCAACCTGTTCGGGTTTTTGATCCCTCGATTCCTTCCGAGAGCGTTTGAGAGGTACTTCAGGGAGAGGGATGAAGTTGGGGCCAAGATGGTAGAGGACAAGCGCTCCGCCGCAGCAAATAAGCCGGAACCATTTGCAAATAAGAAAAGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

279

Amino Acids

30.93

Weight (kDa)

9.89

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 48 - 247 8.4e-81 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 163
AccBSI CCGCTC 2 cut(s) 88, 426
AclWI GGATC 4 cut(s) 60, 73, 557, 697
AcoI YGGCCR 1 cut(s) 186
AcuI CTGAAG 2 cut(s) 313, 724
AfaI GTAC 1 cut(s) 737
AfeI AGCGCT 1 cut(s) 785
AfiI CCNNNNNNNGG 3 cut(s) 163, 278, 360
AflIII ACRYGT 1 cut(s) 384
AjnI CCWGG 2 cut(s) 61, 651
AluBI AGCT 1 cut(s) 154
AluI AGCT 1 cut(s) 154
Alw21I GWGCWC 2 cut(s) 156, 417
AlwI GGATC 4 cut(s) 60, 73, 557, 697
AlwNI CAGNNNCTG 1 cut(s) 466
Aor51HI AGCGCT 1 cut(s) 785
AoxI GGCC 5 cut(s) 165, 186, 193, 250, 762
ApeKI GCWGC 2 cut(s) 318, 794
AspLEI GCGC 2 cut(s) 284, 786
AspS9I GGNCC 2 cut(s) 523, 762
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 2 cut(s) 419, 495
AvaII GGWCC 1 cut(s) 523
BamHI GGATCC 1 cut(s) 65
BanII GRGCYC 3 cut(s) 42, 156, 366
BauI CACGAG 1 cut(s) 161
Bbv12I GWGCWC 2 cut(s) 156, 417
BbvI GCAGC 2 cut(s) 330, 806
BccI CCATC 1 cut(s) 763
BceAI ACGGC 2 cut(s) 5, 295
BciT130I CCWGG 2 cut(s) 63, 653
BcnI CCSGG 1 cut(s) 224
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 2 cut(s) 117, 657
BfoI RGCGCY 2 cut(s) 285, 787
BisI GCNGC 4 cut(s) 319, 374, 792, 795
BlsI GCNGC 4 cut(s) 320, 375, 793, 796
Bme1390I CCNGG 3 cut(s) 63, 224, 653
Bme18I GGWCC 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 523, 762
BmiI GGNNCC 6 cut(s) 39, 67, 352, 525, 763, 810
BmrFI CCNGG 3 cut(s) 63, 224, 653
BpuMI CCSGG 1 cut(s) 224
BsaAI YACGTR 1 cut(s) 385
BsaJI CCNNGG 3 cut(s) 61, 277, 313
BsaXI ACNNNNNCTCC 2 cut(s) 770, 800
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 278, 360
Bse1I ACTGG 3 cut(s) 189, 298, 509
Bse3DI GCAATG 1 cut(s) 527
BseBI CCWGG 2 cut(s) 63, 653
BseDI CCNNGG 3 cut(s) 61, 277, 313
BseGI GGATG 3 cut(s) 576, 628, 757
BseLI CCNNNNNNNGG 3 cut(s) 163, 278, 360
BseMI GCAATG 1 cut(s) 527
BseMII CTCAG 2 cut(s) 314, 462
BseNI ACTGG 3 cut(s) 189, 298, 509
BseXI GCAGC 2 cut(s) 330, 806
BshFI GGCC 5 cut(s) 167, 188, 195, 252, 764
BsiHKAI GWGCWC 2 cut(s) 156, 417
BsiSI CCGG 2 cut(s) 224, 806
BslFI GGGAC 2 cut(s) 185, 509
BslI CCNNNNNNNGG 3 cut(s) 163, 278, 360
BsmFI GGGAC 2 cut(s) 185, 509
BsnI GGCC 5 cut(s) 167, 188, 195, 252, 764
Bsp1286I GDGCHC 4 cut(s) 42, 156, 366, 417
Bsp143I GATC 6 cut(s) 32, 65, 216, 549, 681, 702
Bsp19I CCATGG 1 cut(s) 313
BspANI GGCC 5 cut(s) 167, 188, 195, 252, 764
BspCNI CTCAG 2 cut(s) 313, 463
BspLI GGNNCC 6 cut(s) 39, 67, 352, 525, 763, 810
BspPI GGATC 4 cut(s) 60, 73, 557, 697
BspQI GCTCTTC 1 cut(s) 371
BsrBI CCGCTC 2 cut(s) 88, 426
BsrDI GCAATG 1 cut(s) 527
BsrI ACTGG 3 cut(s) 189, 298, 509
BssECI CCNNGG 3 cut(s) 61, 277, 313
BssMI GATC 6 cut(s) 32, 65, 216, 549, 681, 702
BssSI CACGAG 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 313
Bst2BI CACGAG 1 cut(s) 161
Bst2UI CCWGG 2 cut(s) 63, 653
Bst4CI ACNGT 3 cut(s) 358, 541, 615
Bst6I CTCTTC 1 cut(s) 371
BstBAI YACGTR 1 cut(s) 385
BstC8I GCNNGC 3 cut(s) 378, 417, 603
BstDEI CTNAG 2 cut(s) 300, 471
BstDSI CCRYGG 2 cut(s) 277, 313
BstF5I GGATG 3 cut(s) 576, 628, 757
BstH2I RGCGCY 2 cut(s) 285, 787
BstHHI GCGC 2 cut(s) 284, 786
BstKTI GATC 6 cut(s) 35, 68, 219, 552, 684, 705
BstMBI GATC 6 cut(s) 32, 65, 216, 549, 681, 702
BstMWI GCNNNNNNNGC 3 cut(s) 258, 288, 370
BstNI CCWGG 2 cut(s) 63, 653
BstSCI CCNGG 3 cut(s) 61, 222, 651
BstSFI CTRYAG 2 cut(s) 117, 657
BstV1I GCAGC 2 cut(s) 330, 806
BstX2I RGATCY 2 cut(s) 65, 549
BstYI RGATCY 2 cut(s) 65, 549
BsuRI GGCC 5 cut(s) 167, 188, 195, 252, 764
BtgI CCRYGG 2 cut(s) 277, 313
BtsCI GGATG 3 cut(s) 576, 628, 757
BtsI GCAGTG 1 cut(s) 137
BtsIMutI CAGTG 5 cut(s) 137, 196, 291, 516, 537
Cac8I GCNNGC 3 cut(s) 378, 417, 603
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 2 cut(s) 284, 786
Cfr13I GGNCC 2 cut(s) 523, 762
Csp6I GTAC 1 cut(s) 736
CviAII CATG 3 cut(s) 175, 314, 630
CviQI GTAC 1 cut(s) 736
DdeI CTNAG 2 cut(s) 300, 471
DpnI GATC 6 cut(s) 34, 67, 218, 551, 683, 704
DpnII GATC 6 cut(s) 32, 65, 216, 549, 681, 702
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 371
EarI CTCTTC 1 cut(s) 371
EciI GGCGGA 1 cut(s) 778
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 313
Eco147I AGGCCT 1 cut(s) 252
Eco24I GRGCYC 3 cut(s) 42, 156, 366
Eco47I GGWCC 1 cut(s) 523
Eco47III AGCGCT 1 cut(s) 785
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 2 cut(s) 313, 724
EcoICRI GAGCTC 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 61, 651
EcoT14I CCWWGG 1 cut(s) 313
EcoT38I GRGCYC 3 cut(s) 42, 156, 366
ErhI CCWWGG 1 cut(s) 313
FaeI CATG 3 cut(s) 178, 317, 633
FaiI YATR 6 cut(s) 176, 241, 315, 339, 382, 631
FaqI GGGAC 2 cut(s) 185, 509
FatI CATG 3 cut(s) 174, 313, 629
FauI CCCGC 2 cut(s) 210, 479
Fnu4HI GCNGC 4 cut(s) 319, 374, 792, 795
FokI GGATG 3 cut(s) 583, 635, 764
FriOI GRGCYC 3 cut(s) 42, 156, 366
Fsp4HI GCNGC 4 cut(s) 319, 374, 792, 795
FspBI CTAG 1 cut(s) 30
GlaI GCGC 2 cut(s) 283, 785
GluI GCNGC 4 cut(s) 319, 374, 792, 795
HaeII RGCGCY 2 cut(s) 285, 787
HaeIII GGCC 5 cut(s) 167, 188, 195, 252, 764
HapII CCGG 2 cut(s) 224, 806
HhaI GCGC 2 cut(s) 284, 786
Hin1II CATG 3 cut(s) 178, 317, 633
Hin6I GCGC 2 cut(s) 282, 784
HinP1I GCGC 2 cut(s) 282, 784
HinfI GANTC 3 cut(s) 324, 463, 711
HpaII CCGG 2 cut(s) 224, 806
HphI GGTGA 2 cut(s) 419, 495
Hpy166II GTNNAC 1 cut(s) 137
Hpy188I TCNGA 4 cut(s) 37, 462, 468, 720
Hpy188III TCNNGA 3 cut(s) 302, 544, 679
Hpy8I GTNNAC 1 cut(s) 137
HpyAV CCTTC 3 cut(s) 178, 282, 725
HpyCH4III ACNGT 3 cut(s) 358, 541, 615
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 5 cut(s) 380, 431, 611, 629, 818
HpyF10VI GCNNNNNNNGC 3 cut(s) 258, 288, 370
HpyF3I CTNAG 2 cut(s) 300, 471
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 3 cut(s) 178, 317, 633
HspAI GCGC 2 cut(s) 282, 784
Kzo9I GATC 6 cut(s) 32, 65, 216, 549, 681, 702
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 4 cut(s) 37, 93, 159, 791
Lsp1109I GCAGC 2 cut(s) 330, 806
MaeI CTAG 1 cut(s) 30
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 1 cut(s) 255
MalI GATC 6 cut(s) 34, 67, 218, 551, 683, 704
MbiI CCGCTC 2 cut(s) 88, 426
MboI GATC 6 cut(s) 32, 65, 216, 549, 681, 702
MboII GAAGA 3 cut(s) 211, 319, 358
MflI RGATCY 2 cut(s) 65, 549
MhlI GDGCHC 4 cut(s) 42, 156, 366, 417
MluCI AATT 1 cut(s) 664
MmeI TCCRAC 1 cut(s) 181
MnlI CCTC 9 cut(s) 36, 396, 447, 507, 540, 717, 726, 741, 769
MseI TTAA 2 cut(s) 588, 618
MspI CCGG 2 cut(s) 224, 806
MspR9I CCNGG 3 cut(s) 63, 224, 653
MvaI CCWGG 2 cut(s) 63, 653
MwoI GCNNNNNNNGC 3 cut(s) 258, 288, 370
NciI CCSGG 1 cut(s) 224
NcoI CCATGG 1 cut(s) 313
NdeII GATC 6 cut(s) 32, 65, 216, 549, 681, 702
NlaIII CATG 3 cut(s) 178, 317, 633
NlaIV GGNNCC 6 cut(s) 39, 67, 352, 525, 763, 810
NmeAIII GCCGAG 1 cut(s) 164
NmuCI GTSAC 1 cut(s) 255
PceI AGGCCT 1 cut(s) 252
PciSI GCTCTTC 1 cut(s) 371
PfeI GAWTC 3 cut(s) 324, 463, 711
PflMI CCANNNNNTGG 1 cut(s) 163
PkrI GCNGC 4 cut(s) 320, 375, 793, 796
Ppu21I YACGTR 1 cut(s) 385
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 61, 651
PspGI CCWGG 2 cut(s) 61, 651
PspN4I GGNNCC 6 cut(s) 39, 67, 352, 525, 763, 810
PspPI GGNCC 2 cut(s) 523, 762
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 2 cut(s) 65, 549
RsaI GTAC 1 cut(s) 737
RsaNI GTAC 1 cut(s) 736
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 371
SaqAI TTAA 2 cut(s) 588, 618
SatI GCNGC 4 cut(s) 319, 374, 792, 795
Sau3AI GATC 6 cut(s) 32, 65, 216, 549, 681, 702
Sau96I GGNCC 2 cut(s) 523, 762
ScrFI CCNGG 3 cut(s) 63, 224, 653
SduI GDGCHC 4 cut(s) 42, 156, 366, 417
SetI ASST 7 cut(s) 10, 156, 246, 335, 387, 690, 737
SfcI CTRYAG 2 cut(s) 117, 657
SinI GGWCC 1 cut(s) 523
Sse9I AATT 1 cut(s) 664
SseBI AGGCCT 1 cut(s) 252
SspMI CTAG 1 cut(s) 30
SstI GAGCTC 1 cut(s) 156
StuI AGGCCT 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 61, 222, 651
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 3 cut(s) 358, 541, 615
TaiI ACGT 1 cut(s) 387
TaqI TCGA 2 cut(s) 680, 709
TasI AATT 1 cut(s) 664
TauI GCSGC 2 cut(s) 376, 794
TfiI GAWTC 3 cut(s) 324, 463, 711
Tru1I TTAA 2 cut(s) 588, 618
Tru9I TTAA 2 cut(s) 588, 618
TscAI CASTG 5 cut(s) 144, 196, 298, 516, 544
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 2 cut(s) 318, 794
Tsp45I GTSAC 1 cut(s) 255
TspDTI ATGAA 3 cut(s) 160, 587, 768
TspRI CASTG 5 cut(s) 144, 196, 298, 516, 544
Van91I CCANNNNNTGG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 523
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.