Rw4G025470

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009383
Physical Location & Seq
Forward (+)
51590182 .. 51591448
1267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025470.1

Sequence Viewer

Length: 843 bp
ATGTCTGGCGTATCGGTTGCTGTGGCTCCTAGGTCGGAGCCTGATAAAACTACAACTGCTGGATCACAACACCAGGAAAAGCCACATCGCCAACTGCACCAACAGCGGCAGCAGCCAGCTGTTGGAGGTGTCATGGGGTCATTGCGCGTAATCGAGCTCCAGCTCGTGGCATTCATCATGGTGTTCTCTGCCAGTGGCCTCGTCCCGCTCCTTGATTTGATCTTCCCTGCTTTTGCCTCCGCCTATCTCTTAGTACTGTCGCGCTTGGCCTTCCCATCACATGGTCATGTCTCTTCTGGCGCGCAGGAGATTTTTCAAGGAAGCAAATTCTTTAGACTGTATGTGATTGTGGGAACTACAATTGGGCTGTTCTTGCCTTTGGCCTATGTGTTGGGTGGTTTTGCAAGGGGTGATGAGCACGCTGTGCGCTCAGCAACACCTCACTTGTTCTTGCTCTCCTTTCAGATTCTGACTGAAAATGTCATAAGTGGGTTGTCACTATTTTCGCCACCAGTTAGAGCACTTGTTCCGTTGCTTTACACAGTTAGGAGAATCTTTGTCATCCTTGATTGGATGAAGGATGTCTGGCTTAACAAGACTCTCCCTGCAAATGCACAATTGAAGGATGTTGCATGGTTTTGGTTTGGAAGGACCTTGGCTGTGGCCAACTTCTTATATTTCTCGATCAATTTGTTTGGGTTTTTGATTCCTCGATTTCTTCCAAGGGCCTTTGAGAGGTACTTTAGGGAGAGAGATGAAGTTCATTCCAAGACTGCAGAGGACAAGCGCTCTGCAGCTGTAAATAAATCGCAATCAGCTCCCGCAGATAAGAAGGCCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

31.19

Weight (kDa)

9.93

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 49 - 248 2.2e-81 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 122, 166, 281
AccBSI CCGCTC 1 cut(s) 208
AccII CGCG 3 cut(s) 147, 262, 302
AciI CCGC 4 cut(s) 106, 206, 240, 822
AclWI GGATC 1 cut(s) 70
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 1 cut(s) 326
AdeI CACNNNGTG 1 cut(s) 424
AfaI GTAC 2 cut(s) 255, 740
AfeI AGCGCT 1 cut(s) 788
AfiI CCNNNNNNNGG 4 cut(s) 122, 166, 281, 735
AgsI TTSAA 2 cut(s) 317, 622
AjnI CCWGG 1 cut(s) 72
AluBI AGCT 5 cut(s) 119, 157, 163, 797, 818
AluI AGCT 5 cut(s) 119, 157, 163, 797, 818
Alw21I GWGCWC 3 cut(s) 159, 420, 523
Alw26I GTCTC 1 cut(s) 295
AlwI GGATC 1 cut(s) 70
AlwNI CAGNNNCTG 1 cut(s) 469
Aor51HI AGCGCT 1 cut(s) 788
AoxI GGCC 6 cut(s) 196, 267, 381, 663, 726, 834
ApeKI GCWGC 3 cut(s) 109, 112, 794
ApoI RAATTY 1 cut(s) 326
AspA2I CCTAGG 1 cut(s) 29
AspLEI GCGC 6 cut(s) 147, 264, 302, 304, 429, 789
AspS9I GGNCC 2 cut(s) 651, 726
AsuHPI GGTGA 1 cut(s) 422
AvaII GGWCC 1 cut(s) 651
AvrII CCTAGG 1 cut(s) 29
BalI TGGCCA 1 cut(s) 665
BanII GRGCYC 1 cut(s) 159
BauI CACGAG 1 cut(s) 164
Bbv12I GWGCWC 3 cut(s) 159, 420, 523
BbvI GCAGC 3 cut(s) 121, 124, 806
BccI CCATC 1 cut(s) 283
BciT130I CCWGG 1 cut(s) 74
BcoDI GTCTC 1 cut(s) 295
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 2 cut(s) 774, 792
BfoI RGCGCY 1 cut(s) 790
BisI GCNGC 4 cut(s) 107, 110, 113, 795
BlnI CCTAGG 1 cut(s) 29
BlpI GCTNAGC 1 cut(s) 430
BlsI GCNGC 4 cut(s) 108, 111, 114, 796
BmcAI AGTACT 1 cut(s) 255
Bme1390I CCNGG 1 cut(s) 74
Bme18I GGWCC 1 cut(s) 651
BmgT120I GGNCC 2 cut(s) 651, 726
BmiI GGNNCC 2 cut(s) 27, 39
BmrFI CCNGG 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 143
Bpu1102I GCTNAGC 1 cut(s) 430
BsaJI CCNNGG 3 cut(s) 29, 654, 722
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 166, 281, 735
Bse1I ACTGG 2 cut(s) 192, 512
Bse3DI GCAATG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 74
BseDI CCNNGG 3 cut(s) 29, 654, 722
BseGI GGATG 4 cut(s) 561, 579, 586, 631
BseLI CCNNNNNNNGG 4 cut(s) 122, 166, 281, 735
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 1 cut(s) 444
BseNI ACTGG 2 cut(s) 192, 512
BsePI GCGCGC 1 cut(s) 300
BseXI GCAGC 3 cut(s) 121, 124, 806
BsgI GTGCAG 1 cut(s) 80
Bsh1236I CGCG 3 cut(s) 147, 262, 302
BshFI GGCC 6 cut(s) 198, 269, 383, 665, 728, 836
BsiHKAI GWGCWC 3 cut(s) 159, 420, 523
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 4 cut(s) 122, 166, 281, 735
BsmAI GTCTC 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 188
BsmI GAATGC 1 cut(s) 170
BsnI GGCC 6 cut(s) 198, 269, 383, 665, 728, 836
Bsp1286I GDGCHC 3 cut(s) 159, 420, 523
Bsp143I GATC 3 cut(s) 62, 219, 684
Bsp1720I GCTNAGC 1 cut(s) 430
BspACI CCGC 4 cut(s) 106, 206, 240, 822
BspANI GGCC 6 cut(s) 198, 269, 383, 665, 728, 836
BspCNI CTCAG 1 cut(s) 443
BspFNI CGCG 3 cut(s) 147, 262, 302
BspLI GGNNCC 2 cut(s) 27, 39
BspMAI CTGCAG 2 cut(s) 778, 796
BspPI GGATC 1 cut(s) 70
BsrBI CCGCTC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 140
BsrI ACTGG 2 cut(s) 192, 512
BssECI CCNNGG 3 cut(s) 29, 654, 722
BssHII GCGCGC 1 cut(s) 300
BssMI GATC 3 cut(s) 62, 219, 684
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 3 cut(s) 29, 654, 722
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 1 cut(s) 74
Bst4CI ACNGT 3 cut(s) 258, 339, 544
Bst6I CTCTTC 1 cut(s) 298
BstAPI GCANNNNNTGC 1 cut(s) 424
BstC8I GCNNGC 3 cut(s) 117, 302, 420
BstDEI CTNAG 2 cut(s) 250, 430
BstENI CCTNNNNNAGG 1 cut(s) 733
BstF5I GGATG 4 cut(s) 561, 579, 586, 631
BstFNI CGCG 3 cut(s) 147, 262, 302
BstH2I RGCGCY 1 cut(s) 790
BstHHI GCGC 6 cut(s) 147, 264, 302, 304, 429, 789
BstKTI GATC 3 cut(s) 65, 222, 687
BstMAI GTCTC 1 cut(s) 295
BstMBI GATC 3 cut(s) 62, 219, 684
BstMWI GCNNNNNNNGC 4 cut(s) 103, 112, 373, 424
BstNI CCWGG 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 72
BstSFI CTRYAG 2 cut(s) 774, 792
BstUI CGCG 3 cut(s) 147, 262, 302
BstV1I GCAGC 3 cut(s) 121, 124, 806
BsuRI GGCC 6 cut(s) 198, 269, 383, 665, 728, 836
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 4 cut(s) 561, 579, 586, 631
BtsIMutI CAGTG 1 cut(s) 199
Cac8I GCNNGC 3 cut(s) 117, 302, 420
CaiI CAGNNNCTG 1 cut(s) 469
CfoI GCGC 6 cut(s) 147, 264, 302, 304, 429, 789
Cfr13I GGNCC 2 cut(s) 651, 726
Csp6I GTAC 2 cut(s) 254, 739
CviAII CATG 5 cut(s) 133, 178, 281, 287, 633
CviQI GTAC 2 cut(s) 254, 739
DdeI CTNAG 2 cut(s) 250, 430
DpnI GATC 3 cut(s) 64, 221, 686
DpnII GATC 3 cut(s) 62, 219, 684
DraIII CACNNNGTG 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 663
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
EciI GGCGGA 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 3 cut(s) 29, 654, 722
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 651
Eco47III AGCGCT 1 cut(s) 788
Eco53kI GAGCTC 1 cut(s) 157
EcoICRI GAGCTC 1 cut(s) 157
EcoNI CCTNNNNNAGG 1 cut(s) 733
EcoO109I RGGNCCY 2 cut(s) 651, 726
EcoRII CCWGG 1 cut(s) 72
EcoT14I CCWWGG 3 cut(s) 29, 654, 722
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 3 cut(s) 29, 654, 722
FaeI CATG 5 cut(s) 136, 181, 284, 290, 636
FaiI YATR 9 cut(s) 134, 179, 282, 288, 342, 387, 485, 634, 676
FaqI GGGAC 1 cut(s) 188
FatI CATG 5 cut(s) 132, 177, 280, 286, 632
FauI CCCGC 2 cut(s) 213, 829
Fnu4HI GCNGC 4 cut(s) 107, 110, 113, 795
FokI GGATG 4 cut(s) 548, 586, 593, 638
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 4 cut(s) 107, 110, 113, 795
FspBI CTAG 1 cut(s) 30
GlaI GCGC 6 cut(s) 146, 263, 301, 303, 428, 788
GluI GCNGC 4 cut(s) 107, 110, 113, 795
GsuI CTGGAG 1 cut(s) 143
HaeII RGCGCY 1 cut(s) 790
HaeIII GGCC 6 cut(s) 198, 269, 383, 665, 728, 836
HhaI GCGC 6 cut(s) 147, 264, 302, 304, 429, 789
Hin1II CATG 5 cut(s) 136, 181, 284, 290, 636
Hin6I GCGC 6 cut(s) 145, 262, 300, 302, 427, 787
HinP1I GCGC 6 cut(s) 145, 262, 300, 302, 427, 787
HinfI GANTC 4 cut(s) 466, 552, 598, 706
HphI GGTGA 1 cut(s) 422
Hpy188I TCNGA 3 cut(s) 37, 465, 471
Hpy188III TCNNGA 1 cut(s) 682
HpyAV CCTTC 5 cut(s) 280, 571, 616, 642, 826
HpyCH4III ACNGT 3 cut(s) 258, 339, 544
HpyCH4V TGCA 7 cut(s) 97, 404, 608, 614, 632, 776, 794
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 112, 373, 424
HpyF3I CTNAG 2 cut(s) 250, 430
Hsp92II CATG 5 cut(s) 136, 181, 284, 290, 636
HspAI GCGC 6 cut(s) 145, 262, 300, 302, 427, 787
Kzo9I GATC 3 cut(s) 62, 219, 684
LmnI GCTCC 5 cut(s) 31, 37, 162, 213, 823
Lsp1109I GCAGC 3 cut(s) 121, 124, 806
MaeI CTAG 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 495
MalI GATC 3 cut(s) 64, 221, 686
MbiI CCGCTC 1 cut(s) 208
MboI GATC 3 cut(s) 62, 219, 684
MboII GAAGA 3 cut(s) 214, 285, 710
MfeI CAATTG 2 cut(s) 360, 617
MhlI GDGCHC 3 cut(s) 159, 420, 523
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 4 cut(s) 326, 360, 617, 688
MluNI TGGCCA 1 cut(s) 665
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 2 cut(s) 15, 103
MnlI CCTC 7 cut(s) 119, 209, 247, 450, 720, 729, 772
Mox20I TGGCCA 1 cut(s) 665
MscI TGGCCA 1 cut(s) 665
MseI TTAA 1 cut(s) 591
MslI CAYNNNNRTG 2 cut(s) 179, 285
Msp20I TGGCCA 1 cut(s) 665
MspA1I CMGCKG 3 cut(s) 106, 119, 797
MspR9I CCNGG 1 cut(s) 74
MunI CAATTG 2 cut(s) 360, 617
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 1 cut(s) 74
MvnI CGCG 3 cut(s) 147, 262, 302
MwoI GCNNNNNNNGC 4 cut(s) 103, 112, 373, 424
NdeII GATC 3 cut(s) 62, 219, 684
NlaIII CATG 5 cut(s) 136, 181, 284, 290, 636
NlaIV GGNNCC 2 cut(s) 27, 39
NmuCI GTSAC 1 cut(s) 495
PauI GCGCGC 1 cut(s) 300
PctI GAATGC 1 cut(s) 170
PfeI GAWTC 3 cut(s) 466, 552, 706
PflMI CCANNNNNTGG 3 cut(s) 122, 166, 281
PkrI GCNGC 4 cut(s) 108, 111, 114, 796
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PpuMI RGGWCCY 1 cut(s) 651
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 72
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 2 cut(s) 27, 39
PspPI GGNCC 2 cut(s) 651, 726
PspPPI RGGWCCY 1 cut(s) 651
PstI CTGCAG 2 cut(s) 778, 796
PstNI CAGNNNCTG 1 cut(s) 469
PteI GCGCGC 1 cut(s) 300
PvuII CAGCTG 2 cut(s) 119, 797
RsaI GTAC 2 cut(s) 255, 740
RsaNI GTAC 2 cut(s) 254, 739
RseI CAYNNNNRTG 2 cut(s) 179, 285
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 4 cut(s) 107, 110, 113, 795
Sau3AI GATC 3 cut(s) 62, 219, 684
Sau96I GGNCC 2 cut(s) 651, 726
ScaI AGTACT 1 cut(s) 255
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 1 cut(s) 74
SduI GDGCHC 3 cut(s) 159, 420, 523
SfcI CTRYAG 2 cut(s) 774, 792
SinI GGWCC 1 cut(s) 651
SmiMI CAYNNNNRTG 2 cut(s) 179, 285
Sse9I AATT 4 cut(s) 326, 360, 617, 688
SsiI CCGC 4 cut(s) 106, 206, 240, 822
SspMI CTAG 1 cut(s) 30
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 72
StyI CCWWGG 3 cut(s) 29, 654, 722
TaaI ACNGT 3 cut(s) 258, 339, 544
TaqI TCGA 3 cut(s) 153, 683, 712
TasI AATT 4 cut(s) 326, 360, 617, 688
TatI WGTACW 1 cut(s) 253
TauI GCSGC 1 cut(s) 109
TfiI GAWTC 3 cut(s) 466, 552, 706
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 1 cut(s) 495
TseI GCWGC 3 cut(s) 109, 112, 794
Tsp45I GTSAC 1 cut(s) 495
TspDTI ATGAA 4 cut(s) 163, 590, 752, 771
TspGWI ACGGA 1 cut(s) 519
TspRI CASTG 1 cut(s) 199
Van91I CCANNNNNTGG 3 cut(s) 122, 166, 281
VpaK11BI GGWCC 1 cut(s) 651
XagI CCTNNNNNAGG 1 cut(s) 733
XapI RAATTY 1 cut(s) 326
XmaJI CCTAGG 1 cut(s) 29
XspI CTAG 1 cut(s) 30
ZrmI AGTACT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.