AT1G29950

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009324
Physical Location & Seq
Forward (+)
10491698 .. 10494598
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G29950.2

Sequence Viewer

Length: 756 bp
ATGCAGAACAATCAGTTTCCTCACTTCTCAGATGAAGTGGGTGACAGAAATATGCACAACCCGTATGCATCAGGGTCATCCTATGATGCTTTGTTCCCACCATGTGCAAAGTTGCCATACCATGGTGTTGAACTTCAACCGTCTGCGGTCTGTCCAAAGAACTTTGTCATCTTCGATCAAACATATGACCGCAGCCAAGTGATGTACCATCCTGAGCTGACTCATAAGCTCATGAATACCCCTTCGTTGAACAATTTAGCTTCGACGTTTCAGAACGAGTATGTTGGGGGAAGTTATGGTAACTATGGTAACTATGAGCAAGAAGTATCCTCTTCTTATCAAGAAGATCCAAATGAGATCGATGCTCTCTTGAGCGCAGATGAAGATTATGAAGAGAATGATGATAATGAAGGTGAAGAGGATGGTGGTGATTCAGAAGAAGTCAGCACTGCTCGTACTTCTTCCAGGGATTATGGAAACACCACAGCAGAATCTTGTTGTTCCAGTTATGGTTACAACAACAACAACAACAACAACTCAAGGAAGCAGAGTTTATCGGGCAGTGCTAGTAGTAGTAACAATGATGGGAAAGGACGTAAAAAGATGAAGAAGATGATGGGAGTATTGAGGAGAATTGTCCCTGGAGGAGAACAGATGAATACAGCTTGCGTTCTTGATGAAGCTGTTCAGTATCTCAAGTCACTTAAAATCGAAGCTCAGAAACTTGGCGTTGGACATTTCTCAAACCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

27.82

Weight (kDa)

4.69

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 193 - 251 4.2e-27 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 122
AciI CCGC 2 cut(s) 146, 190
AclWI GGATC 1 cut(s) 341
AdeI CACNNNGTG 1 cut(s) 104
AfaI GTAC 2 cut(s) 206, 457
AfiI CCNNNNNNNGG 1 cut(s) 122
AgsI TTSAA 3 cut(s) 131, 137, 250
AjnI CCWGG 2 cut(s) 464, 640
AluBI AGCT 6 cut(s) 217, 229, 260, 665, 683, 716
AluI AGCT 6 cut(s) 217, 229, 260, 665, 683, 716
AlwI GGATC 1 cut(s) 341
ApeKI GCWGC 1 cut(s) 192
Asp700I GAANNNNTTC 1 cut(s) 684
AspLEI GCGC 1 cut(s) 377
AsuHPI GGTGA 3 cut(s) 53, 425, 440
BbvI GCAGC 1 cut(s) 204
BccI CCATC 4 cut(s) 216, 416, 578, 610
BciT130I CCWGG 2 cut(s) 466, 642
BciVI GTATCC 1 cut(s) 337
BfaI CTAG 1 cut(s) 567
BfuI GTATCC 1 cut(s) 337
BisI GCNGC 1 cut(s) 193
BlsI GCNGC 1 cut(s) 194
Bme1390I CCNGG 2 cut(s) 466, 642
BmrFI CCNGG 2 cut(s) 466, 642
BmsI GCATC 3 cut(s) 76, 77, 352
BpmI CTGGAG 1 cut(s) 663
Bpu10I CCTNAGC 1 cut(s) 213
BpuEI CTTGAG 3 cut(s) 391, 523, 680
Bsa29I ATCGAT 1 cut(s) 360
BsaJI CCNNGG 3 cut(s) 121, 465, 640
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 504
BseBI CCWGG 2 cut(s) 466, 642
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 3 cut(s) 121, 465, 640
BseGI GGATG 3 cut(s) 77, 208, 427
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMII CTCAG 3 cut(s) 42, 204, 731
BseNI ACTGG 1 cut(s) 504
BseRI GAGGAG 2 cut(s) 643, 660
BseXI GCAGC 1 cut(s) 204
BshVI ATCGAT 1 cut(s) 360
BslFI GGGAC 1 cut(s) 623
BslI CCNNNNNNNGG 1 cut(s) 122
BsmFI GGGAC 1 cut(s) 623
Bsp143I GATC 3 cut(s) 175, 346, 357
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 2 cut(s) 146, 190
BspCNI CTCAG 3 cut(s) 41, 205, 730
BspDI ATCGAT 1 cut(s) 360
BspHI TCATGA 1 cut(s) 231
BspPI GGATC 1 cut(s) 341
BsrI ACTGG 1 cut(s) 504
BssECI CCNNGG 3 cut(s) 121, 465, 640
BssMI GATC 3 cut(s) 175, 346, 357
BssT1I CCWWGG 1 cut(s) 121
Bst2UI CCWGG 2 cut(s) 466, 642
Bst4CI ACNGT 1 cut(s) 141
Bst6I CTCTTC 3 cut(s) 337, 387, 411
BstC8I GCNNGC 1 cut(s) 667
BstDEI CTNAG 3 cut(s) 28, 213, 717
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 3 cut(s) 77, 208, 427
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 3 cut(s) 178, 349, 360
BstMBI GATC 3 cut(s) 175, 346, 357
BstNI CCWGG 2 cut(s) 466, 642
BstSCI CCNGG 2 cut(s) 464, 640
BstV1I GCAGC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 360
BsuI GTATCC 1 cut(s) 337
BsuTUI ATCGAT 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 121
BtsCI GGATG 3 cut(s) 77, 208, 427
BtsI GCAGTG 2 cut(s) 447, 568
BtsIMutI CAGTG 2 cut(s) 447, 568
Cac8I GCNNGC 1 cut(s) 667
CciI TCATGA 1 cut(s) 231
CfoI GCGC 1 cut(s) 377
ClaI ATCGAT 1 cut(s) 360
Csp6I GTAC 2 cut(s) 205, 456
CviAII CATG 3 cut(s) 102, 122, 232
CviJI RGCY 7 cut(s) 195, 217, 229, 260, 665, 683, 716
CviKI_1 RGCY 7 cut(s) 195, 217, 229, 260, 665, 683, 716
CviQI GTAC 2 cut(s) 205, 456
DdeI CTNAG 3 cut(s) 28, 213, 717
DpnI GATC 3 cut(s) 177, 348, 359
DpnII GATC 3 cut(s) 175, 346, 357
DraIII CACNNNGTG 1 cut(s) 104
Eam1104I CTCTTC 3 cut(s) 337, 387, 411
EarI CTCTTC 3 cut(s) 337, 387, 411
Eco130I CCWWGG 1 cut(s) 121
EcoRII CCWGG 2 cut(s) 464, 640
EcoT14I CCWWGG 1 cut(s) 121
EcoT22I ATGCAT 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 121
FaeI CATG 3 cut(s) 105, 125, 235
FaqI GGGAC 1 cut(s) 623
FatI CATG 3 cut(s) 101, 121, 231
FauNDI CATATG 1 cut(s) 184
Fnu4HI GCNGC 1 cut(s) 193
FokI GGATG 3 cut(s) 64, 195, 434
Fsp4HI GCNGC 1 cut(s) 193
FspBI CTAG 1 cut(s) 567
GlaI GCGC 1 cut(s) 376
GluI GCNGC 1 cut(s) 193
GsuI CTGGAG 1 cut(s) 663
HhaI GCGC 1 cut(s) 377
Hin1II CATG 3 cut(s) 105, 125, 235
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HinfI GANTC 3 cut(s) 220, 431, 491
HphI GGTGA 3 cut(s) 53, 425, 440
Hpy188I TCNGA 4 cut(s) 31, 273, 436, 720
Hpy188III TCNNGA 6 cut(s) 212, 232, 341, 370, 674, 753
Hpy99I CGWCG 1 cut(s) 268
HpyAV CCTTC 2 cut(s) 252, 404
HpyCH4III ACNGT 1 cut(s) 141
HpyCH4IV ACGT 2 cut(s) 266, 595
HpyCH4V TGCA 4 cut(s) 4, 55, 68, 107
HpyF3I CTNAG 3 cut(s) 28, 213, 717
HpySE526I ACGT 2 cut(s) 266, 595
Hsp92II CATG 3 cut(s) 105, 125, 235
HspAI GCGC 1 cut(s) 375
Kzo9I GATC 3 cut(s) 175, 346, 357
LpnPI CCDG 7 cut(s) 57, 225, 451, 478, 517, 627, 654
Lsp1109I GCAGC 1 cut(s) 204
LweI GCATC 3 cut(s) 76, 77, 352
MaeI CTAG 1 cut(s) 567
MaeII ACGT 2 cut(s) 266, 595
MaeIII GTNAC 6 cut(s) 41, 299, 308, 512, 575, 699
MalI GATC 3 cut(s) 177, 348, 359
MboI GATC 3 cut(s) 175, 346, 357
MflI RGATCY 1 cut(s) 346
MluCI AATT 2 cut(s) 253, 633
MlyI GAGTC 1 cut(s) 214
MmeI TCCRAC 1 cut(s) 712
MnlI CCTC 5 cut(s) 30, 340, 412, 621, 638
Mph1103I ATGCAT 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 684
MseI TTAA 1 cut(s) 705
MspR9I CCNGG 2 cut(s) 466, 642
MvaI CCWGG 2 cut(s) 466, 642
NcoI CCATGG 1 cut(s) 121
NdeI CATATG 1 cut(s) 184
NdeII GATC 3 cut(s) 175, 346, 357
NlaIII CATG 3 cut(s) 105, 125, 235
NmuCI GTSAC 2 cut(s) 41, 699
NsiI ATGCAT 1 cut(s) 70
PagI TCATGA 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 2 cut(s) 431, 491
PflMI CCANNNNNTGG 1 cut(s) 122
PkrI GCNGC 1 cut(s) 194
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
Psp6I CCWGG 2 cut(s) 464, 640
PspGI CCWGG 2 cut(s) 464, 640
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 2 cut(s) 206, 457
RsaNI GTAC 2 cut(s) 205, 456
SaqAI TTAA 1 cut(s) 705
SatI GCNGC 1 cut(s) 193
Sau3AI GATC 3 cut(s) 175, 346, 357
SchI GAGTC 1 cut(s) 214
ScrFI CCNGG 2 cut(s) 466, 642
SetI ASST 9 cut(s) 219, 231, 262, 269, 415, 598, 667, 685, 718
SfaNI GCATC 3 cut(s) 76, 77, 352
SmlI CTYRAG 3 cut(s) 370, 538, 695
SmoI CTYRAG 3 cut(s) 370, 538, 695
Sse9I AATT 2 cut(s) 253, 633
SsiI CCGC 2 cut(s) 146, 190
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 2 cut(s) 464, 640
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 1 cut(s) 141
TaiI ACGT 2 cut(s) 269, 598
TaqI TCGA 4 cut(s) 174, 263, 360, 711
TasI AATT 2 cut(s) 253, 633
TfiI GAWTC 2 cut(s) 431, 491
Tru1I TTAA 1 cut(s) 705
Tru9I TTAA 1 cut(s) 705
TscAI CASTG 2 cut(s) 454, 568
TseFI GTSAC 2 cut(s) 41, 699
TseI GCWGC 1 cut(s) 192
Tsp45I GTSAC 2 cut(s) 41, 699
TspDTI ATGAA 8 cut(s) 48, 248, 396, 405, 423, 620, 671, 693
TspRI CASTG 2 cut(s) 454, 568
Van91I CCANNNNNTGG 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 1 cut(s) 567
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.