MD10G1264900.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009324
Physical Location & Seq
Reverse (-)
35859220 .. 35860200
981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1264900.v1.1.491

Sequence Viewer

Length: 732 bp
ATGCAGAGTGACCAGCAATTTGACCCCAACAAGGCAGCTCCGCCGTTTTCAAATCAAGTGGGTAATAATTACATGCATATTCCAGTTGCTTCTGGGTGTGGGGCAGTTTTACCTAATGACGCAAATCACTTTAGACCTTTCCATGGTGTTGAATTTCAAACCTCTAGCATCTGCCCGAAGAATTTCATTATCTTCGACCAGACTGATCATCGAAGTCAGATTATGTTCAATCCTGAAATTTCCCACAAAATTACTGGTCCTGCCTTTAACCTCTGTGCAGCTTACATCCAAGACAATCTGGGATTGAACAAGGGAAATATTGACAATAGAGAAGCATCGTCTACTCTGAAGGAGGACTCCGATGATATTGATGCATTGTTGAGCCTGGAAGAGGAAGAGCTGGAAGATTATGATGAGGAAGAGGTCAGTACAGCACGAACTCGTGGAAATTATGGGAGCTCATTCTCTGATTCTTGCTCCAGTTATGGTTTGAAAACCAAGAAGGAAAGGCTGTGTTCTTCACTTGAAAAATCTACTGCCATTGGTAGCAGTAGCAGCTGCAATAGTGAAAGGAAACGCCAAAAAATGAAGAAGATGGTGAGGGCGCTGAGAGGGATTGTTCCTGGTGCGAATGAAATGAACACTGTCGCCGTTCTAGACGAAGCTGTTCAGTACTTGAAATCTCTCAAGGTTGAGCTGCAGAAGCTTGGAGTTGAGAATTTGAACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

244

Amino Acids

26.91

Weight (kDa)

5.14

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH_SAC51 PF23173 188 - 240 2.9e-18 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 341
AciI CCGC 1 cut(s) 41
AcsI RAATTY 4 cut(s) 152, 181, 237, 718
AcuI CTGAAG 1 cut(s) 368
AfaI GTAC 2 cut(s) 430, 674
AfiI CCNNNNNNNGG 3 cut(s) 31, 143, 391
AgsI TTSAA 9 cut(s) 51, 152, 158, 229, 307, 493, 527, 679, 724
AjnI CCWGG 2 cut(s) 384, 622
AluBI AGCT 8 cut(s) 38, 281, 400, 459, 558, 665, 697, 706
AluI AGCT 8 cut(s) 38, 281, 400, 459, 558, 665, 697, 706
Alw21I GWGCWC 1 cut(s) 461
ApeKI GCWGC 5 cut(s) 35, 278, 555, 558, 697
ApoI RAATTY 4 cut(s) 152, 181, 237, 718
ArsI GACNNNNNNTTYG 1 cut(s) 34
Asp700I GAANNNNTTC 2 cut(s) 182, 666
AspLEI GCGC 1 cut(s) 607
AspS9I GGNCC 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 610
AvaII GGWCC 1 cut(s) 257
BanII GRGCYC 1 cut(s) 461
BauI CACGAG 1 cut(s) 441
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 5 cut(s) 47, 290, 545, 567, 684
BccI CCATC 1 cut(s) 589
BceAI ACGGC 2 cut(s) 28, 635
BciT130I CCWGG 2 cut(s) 386, 624
BclI TGATCA 1 cut(s) 205
BfaI CTAG 2 cut(s) 165, 656
BfmI CTRYAG 1 cut(s) 698
BfoI RGCGCY 1 cut(s) 608
BisI GCNGC 5 cut(s) 36, 279, 556, 559, 698
BlsI GCNGC 5 cut(s) 37, 280, 557, 560, 699
BmcAI AGTACT 1 cut(s) 674
Bme1390I CCNGG 2 cut(s) 386, 624
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 257
BmrFI CCNGG 2 cut(s) 386, 624
BmsI GCATC 3 cut(s) 177, 344, 361
BpmI CTGGAG 1 cut(s) 463
BpuEI CTTGAG 1 cut(s) 671
BsaJI CCNNGG 1 cut(s) 142
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 143, 391
Bse1I ACTGG 3 cut(s) 83, 259, 480
BseBI CCWGG 2 cut(s) 386, 624
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 1 cut(s) 285
BseLI CCNNNNNNNGG 3 cut(s) 31, 143, 391
BseMII CTCAG 1 cut(s) 599
BseNI ACTGG 3 cut(s) 83, 259, 480
BseXI GCAGC 5 cut(s) 47, 290, 545, 567, 684
BsgI GTGCAG 1 cut(s) 297
BsiHKAI GWGCWC 1 cut(s) 461
BslI CCNNNNNNNGG 3 cut(s) 31, 143, 391
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 1 cut(s) 205
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 1 cut(s) 41
BspCNI CTCAG 1 cut(s) 600
BspMAI CTGCAG 1 cut(s) 702
BspQI GCTCTTC 1 cut(s) 390
BsrI ACTGG 3 cut(s) 83, 259, 480
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 1 cut(s) 205
BssSI CACGAG 1 cut(s) 441
BssT1I CCWWGG 1 cut(s) 142
Bst2BI CACGAG 1 cut(s) 441
Bst2UI CCWGG 2 cut(s) 386, 624
Bst4CI ACNGT 1 cut(s) 646
Bst6I CTCTTC 3 cut(s) 384, 390, 414
BstDEI CTNAG 1 cut(s) 608
BstDSI CCRYGG 1 cut(s) 142
BstENI CCTNNNNNAGG 1 cut(s) 389
BstF5I GGATG 1 cut(s) 285
BstH2I RGCGCY 1 cut(s) 608
BstHHI GCGC 1 cut(s) 607
BstKTI GATC 1 cut(s) 208
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 2 cut(s) 555, 703
BstNI CCWGG 2 cut(s) 386, 624
BstNSI RCATGY 1 cut(s) 76
BstSCI CCNGG 2 cut(s) 384, 622
BstSFI CTRYAG 1 cut(s) 698
BstV1I GCAGC 5 cut(s) 47, 290, 545, 567, 684
BtgI CCRYGG 1 cut(s) 142
BtsCI GGATG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 642
CfoI GCGC 1 cut(s) 607
Cfr13I GGNCC 1 cut(s) 257
CseI GACGC 1 cut(s) 128
Csp6I GTAC 2 cut(s) 429, 673
CspCI CAANNNNNGTGG 2 cut(s) 39, 74
CviAII CATG 2 cut(s) 73, 143
CviQI GTAC 2 cut(s) 429, 673
DdeI CTNAG 1 cut(s) 608
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
Eam1104I CTCTTC 3 cut(s) 384, 390, 414
EarI CTCTTC 3 cut(s) 384, 390, 414
EciI GGCGGA 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 459
Eco130I CCWWGG 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 461
Eco47I GGWCC 1 cut(s) 257
Eco53kI GAGCTC 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 459
EcoNI CCTNNNNNAGG 1 cut(s) 389
EcoRII CCWGG 2 cut(s) 384, 622
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 2 cut(s) 78, 376
EcoT38I GRGCYC 1 cut(s) 461
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 2 cut(s) 76, 146
FaiI YATR 7 cut(s) 74, 78, 144, 224, 411, 453, 486
FatI CATG 2 cut(s) 72, 142
FbaI TGATCA 1 cut(s) 205
FblI GTMKAC 1 cut(s) 341
Fnu4HI GCNGC 5 cut(s) 36, 279, 556, 559, 698
FokI GGATG 1 cut(s) 272
FriOI GRGCYC 1 cut(s) 461
Fsp4HI GCNGC 5 cut(s) 36, 279, 556, 559, 698
FspBI CTAG 2 cut(s) 165, 656
GlaI GCGC 1 cut(s) 606
GluI GCNGC 5 cut(s) 36, 279, 556, 559, 698
GsuI CTGGAG 1 cut(s) 463
HaeII RGCGCY 1 cut(s) 608
HgaI GACGC 1 cut(s) 128
HhaI GCGC 1 cut(s) 607
Hin1II CATG 2 cut(s) 76, 146
Hin6I GCGC 1 cut(s) 605
HinP1I GCGC 1 cut(s) 605
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 2 cut(s) 356, 470
HphI GGTGA 1 cut(s) 610
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 4 cut(s) 219, 348, 361, 469
Hpy188III TCNNGA 2 cut(s) 233, 656
Hpy8I GTNNAC 1 cut(s) 342
HpyAV CCTTC 2 cut(s) 343, 496
HpyCH4III ACNGT 1 cut(s) 646
HpyCH4V TGCA 6 cut(s) 4, 76, 278, 374, 561, 700
HpyF10VI GCNNNNNNNGC 2 cut(s) 555, 703
HpyF3I CTNAG 1 cut(s) 608
Hsp92II CATG 2 cut(s) 76, 146
HspAI GCGC 1 cut(s) 605
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 1 cut(s) 205
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 3 cut(s) 43, 456, 482
Lsp1109I GCAGC 5 cut(s) 47, 290, 545, 567, 684
LweI GCATC 3 cut(s) 177, 344, 361
MaeI CTAG 2 cut(s) 165, 656
MaeIII GTNAC 1 cut(s) 8
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 9 cut(s) 184, 190, 401, 407, 416, 431, 510, 601, 604
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 8 cut(s) 17, 67, 152, 181, 237, 249, 448, 718
MlyI GAGTC 1 cut(s) 350
MnlI CCTC 8 cut(s) 172, 281, 346, 385, 409, 415, 594, 605
Mph1103I ATGCAT 2 cut(s) 78, 376
MroXI GAANNNNTTC 2 cut(s) 182, 666
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 558
MspR9I CCNGG 2 cut(s) 386, 624
MvaI CCWGG 2 cut(s) 386, 624
MwoI GCNNNNNNNGC 2 cut(s) 555, 703
NcoI CCATGG 1 cut(s) 142
NdeII GATC 1 cut(s) 205
NlaIII CATG 2 cut(s) 76, 146
NmuCI GTSAC 1 cut(s) 8
NsiI ATGCAT 2 cut(s) 78, 376
NspI RCATGY 1 cut(s) 76
PciSI GCTCTTC 1 cut(s) 390
PdmI GAANNNNTTC 2 cut(s) 182, 666
PfeI GAWTC 1 cut(s) 470
PkrI GCNGC 5 cut(s) 37, 280, 557, 560, 699
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
Psp124BI GAGCTC 1 cut(s) 461
Psp6I CCWGG 2 cut(s) 384, 622
PspGI CCWGG 2 cut(s) 384, 622
PspPI GGNCC 1 cut(s) 257
PstI CTGCAG 1 cut(s) 702
PvuII CAGCTG 1 cut(s) 558
RsaI GTAC 2 cut(s) 430, 674
RsaNI GTAC 2 cut(s) 429, 673
SacI GAGCTC 1 cut(s) 461
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 5 cut(s) 36, 279, 556, 559, 698
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 1 cut(s) 257
ScaI AGTACT 1 cut(s) 674
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 2 cut(s) 386, 624
SduI GDGCHC 1 cut(s) 461
SfaNI GCATC 3 cut(s) 177, 344, 361
SfcI CTRYAG 1 cut(s) 698
SinI GGWCC 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
Sse9I AATT 8 cut(s) 17, 67, 152, 181, 237, 249, 448, 718
SsiI CCGC 1 cut(s) 41
SspI AATATT 1 cut(s) 319
SspMI CTAG 2 cut(s) 165, 656
SstI GAGCTC 1 cut(s) 461
StyD4I CCNGG 2 cut(s) 384, 622
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 1 cut(s) 646
TaqI TCGA 2 cut(s) 195, 211
TasI AATT 8 cut(s) 17, 67, 152, 181, 237, 249, 448, 718
TatI WGTACW 2 cut(s) 428, 672
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 649
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 5 cut(s) 35, 278, 555, 558, 697
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 4 cut(s) 175, 602, 648, 653
TspRI CASTG 1 cut(s) 649
VpaK11BI GGWCC 1 cut(s) 257
XagI CCTNNNNNAGG 1 cut(s) 389
XapI RAATTY 4 cut(s) 152, 181, 237, 718
XbaI TCTAGA 1 cut(s) 655
XceI RCATGY 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 341
XmnI GAANNNNTTC 2 cut(s) 182, 666
XspI CTAG 2 cut(s) 165, 656
ZrmI AGTACT 1 cut(s) 674
Zsp2I ATGCAT 2 cut(s) 78, 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.